TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Špirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Lazić, Vesna
AU  - Žižak, Željko
AU  - Vunduk, Jovana
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7297
AB  - Growing mushrooms means meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Customized strains adapted to local conditions are urgently needed. Before introducing new species to the strain development pipeline, the chemical characterization and biological activity of wild ones need to be assessed. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated including: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and anti-angiotensin converting enzyme (ACE). The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to normal cells. The evaluated species provided a solid foundation for commercial development while keeping local ecology in mind.
PB  - MDPI
T2  - Molecules
T1  - Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans
VL  - 29
IS  - 2
SP  - 314
DO  - 10.3390/molecules29020314
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Špirović Trifunović, Bojana and Miletić, Srđan and Lazić, Vesna and Žižak, Željko and Vunduk, Jovana",
year = "2024",
abstract = "Growing mushrooms means meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Customized strains adapted to local conditions are urgently needed. Before introducing new species to the strain development pipeline, the chemical characterization and biological activity of wild ones need to be assessed. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated including: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and anti-angiotensin converting enzyme (ACE). The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to normal cells. The evaluated species provided a solid foundation for commercial development while keeping local ecology in mind.",
publisher = "MDPI",
journal = "Molecules",
title = "Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans",
volume = "29",
number = "2",
pages = "314",
doi = "10.3390/molecules29020314"
}

Kozarski, M., Klaus, A., Špirović Trifunović, B., Miletić, S., Lazić, V., Žižak, Ž.,& Vunduk, J.. (2024). Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans. in Molecules
MDPI., 29(2), 314.
https://doi.org/10.3390/molecules29020314

Kozarski M, Klaus A, Špirović Trifunović B, Miletić S, Lazić V, Žižak Ž, Vunduk J. Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans. in Molecules. 2024;29(2):314.
doi:10.3390/molecules29020314 .

Kozarski, Maja, Klaus, Anita, Špirović Trifunović, Bojana, Miletić, Srđan, Lazić, Vesna, Žižak, Željko, Vunduk, Jovana, "Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans" in Molecules, 29, no. 2 (2024):314,
https://doi.org/10.3390/molecules29020314 . .

TY  - JOUR
AU  - Ognjanović, Miloš
AU  - Jaćimović, Željko
AU  - Kosović-Perutović, Milica
AU  - Besu Žižak, Irina
AU  - Stanojković, Tatjana
AU  - Žižak, Željko
AU  - Dojčinović, Biljana
AU  - Stanković, Dalibor M.
AU  - Antić, Bratislav
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6420
AB  - Partial cation substitution can significantly change the physical properties of parent compounds. By controlling the chemical composition and knowing the mutual relationship between composition and physical properties, it is possible to tailor the properties of materials to those that are superior for desired technological application. Using the polyol synthesis procedure, a series of yttrium-substituted iron oxide nanoconstructs, γ-Fe2−xYxO3 (YIONs), was prepared. It was found that Y3+ could substitute Fe3+ in the crystal structures of maghemite (γ-Fe2O3) up to a limited concentration of ~1.5% (γ-Fe1.969Y0.031O3). Analysis of TEM micrographs showed that crystallites or particles were aggregated in flower-like structures with diameters from 53.7 ± 6.2 nm to 97.3 ± 37.0 nm, depending on yttrium concentration. To be investigated for potential applications as magnetic hyperthermia agents, YIONs were tested twice: their heating efficiency was tested and their toxicity was investigated. The Specific Absorption Rate (SAR) values were in the range of 32.6 W/g to 513 W/g and significantly decreased with increased yttrium concentration in the samples. Intrinsic loss power (ILP) for γ-Fe2O3 and γ-Fe1.995Y0.005O3 were ~8–9 nH·m2/Kg, which pointed to their excellent heating efficiency. IC50 values of investigated samples against cancer (HeLa) and normal (MRC-5) cells decreased with increased yttrium concentration and were higher than ~300 μg/mL. The samples of γ-Fe2−xYxO3 did not show a genotoxic effect. The results of toxicity studies show that YIONs are suitable for further in vitro/in vivo studies toward to their potential medical applications, while results of heat generation point to their potential use in magnetic hyperthermia cancer treatment or use as self-heating systems for other technological applications such as catalysis.
T2  - Nanomaterials
T1  - Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity
VL  - 13
IS  - 5
SP  - 870
DO  - 10.3390/nano13050870
ER  - 

@article{
author = "Ognjanović, Miloš and Jaćimović, Željko and Kosović-Perutović, Milica and Besu Žižak, Irina and Stanojković, Tatjana and Žižak, Željko and Dojčinović, Biljana and Stanković, Dalibor M. and Antić, Bratislav",
year = "2023",
abstract = "Partial cation substitution can significantly change the physical properties of parent compounds. By controlling the chemical composition and knowing the mutual relationship between composition and physical properties, it is possible to tailor the properties of materials to those that are superior for desired technological application. Using the polyol synthesis procedure, a series of yttrium-substituted iron oxide nanoconstructs, γ-Fe2−xYxO3 (YIONs), was prepared. It was found that Y3+ could substitute Fe3+ in the crystal structures of maghemite (γ-Fe2O3) up to a limited concentration of ~1.5% (γ-Fe1.969Y0.031O3). Analysis of TEM micrographs showed that crystallites or particles were aggregated in flower-like structures with diameters from 53.7 ± 6.2 nm to 97.3 ± 37.0 nm, depending on yttrium concentration. To be investigated for potential applications as magnetic hyperthermia agents, YIONs were tested twice: their heating efficiency was tested and their toxicity was investigated. The Specific Absorption Rate (SAR) values were in the range of 32.6 W/g to 513 W/g and significantly decreased with increased yttrium concentration in the samples. Intrinsic loss power (ILP) for γ-Fe2O3 and γ-Fe1.995Y0.005O3 were ~8–9 nH·m2/Kg, which pointed to their excellent heating efficiency. IC50 values of investigated samples against cancer (HeLa) and normal (MRC-5) cells decreased with increased yttrium concentration and were higher than ~300 μg/mL. The samples of γ-Fe2−xYxO3 did not show a genotoxic effect. The results of toxicity studies show that YIONs are suitable for further in vitro/in vivo studies toward to their potential medical applications, while results of heat generation point to their potential use in magnetic hyperthermia cancer treatment or use as self-heating systems for other technological applications such as catalysis.",
journal = "Nanomaterials",
title = "Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity",
volume = "13",
number = "5",
pages = "870",
doi = "10.3390/nano13050870"
}

Ognjanović, M., Jaćimović, Ž., Kosović-Perutović, M., Besu Žižak, I., Stanojković, T., Žižak, Ž., Dojčinović, B., Stanković, D. M.,& Antić, B.. (2023). Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity. in Nanomaterials, 13(5), 870.
https://doi.org/10.3390/nano13050870

Ognjanović M, Jaćimović Ž, Kosović-Perutović M, Besu Žižak I, Stanojković T, Žižak Ž, Dojčinović B, Stanković DM, Antić B. Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity. in Nanomaterials. 2023;13(5):870.
doi:10.3390/nano13050870 .

Ognjanović, Miloš, Jaćimović, Željko, Kosović-Perutović, Milica, Besu Žižak, Irina, Stanojković, Tatjana, Žižak, Željko, Dojčinović, Biljana, Stanković, Dalibor M., Antić, Bratislav, "Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity" in Nanomaterials, 13, no. 5 (2023):870,
https://doi.org/10.3390/nano13050870 . .

TY  - GEN
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Špirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Lazić, Vesna
AU  - Žižak, Željko
AU  - Vunduk, Jovana
PY  - 2023
UR  - https://www.preprints.org/manuscript/202311.1765/v1
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7057
AB  - Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.
T2  - Preprints
T1  - Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation
SP  - 2023111765
DO  - 10.20944/preprints202311.1765.v1
ER  - 

@misc{
author = "Kozarski, Maja and Klaus, Anita and Špirović Trifunović, Bojana and Miletić, Srđan and Lazić, Vesna and Žižak, Željko and Vunduk, Jovana",
year = "2023",
abstract = "Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.",
journal = "Preprints",
title = "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation",
pages = "2023111765",
doi = "10.20944/preprints202311.1765.v1"
}

Kozarski, M., Klaus, A., Špirović Trifunović, B., Miletić, S., Lazić, V., Žižak, Ž.,& Vunduk, J.. (2023). Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation. in Preprints, 2023111765.
https://doi.org/10.20944/preprints202311.1765.v1

Kozarski M, Klaus A, Špirović Trifunović B, Miletić S, Lazić V, Žižak Ž, Vunduk J. Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation. in Preprints. 2023;:2023111765.
doi:10.20944/preprints202311.1765.v1 .

Kozarski, Maja, Klaus, Anita, Špirović Trifunović, Bojana, Miletić, Srđan, Lazić, Vesna, Žižak, Željko, Vunduk, Jovana, "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation" in Preprints (2023):2023111765,
https://doi.org/10.20944/preprints202311.1765.v1 . .

TY  - DATA
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Špirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Lazić, Vesna
AU  - Žižak, Željko
AU  - Vunduk, Jovana
PY  - 2023
UR  - https://www.preprints.org/manuscript/202311.1765/v1
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7057
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7058
AB  - Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.
T2  - Preprints
T1  - Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation"
DO  - 10.20944/preprints202311.1765.v1
ER  - 

@misc{
author = "Kozarski, Maja and Klaus, Anita and Špirović Trifunović, Bojana and Miletić, Srđan and Lazić, Vesna and Žižak, Željko and Vunduk, Jovana",
year = "2023",
abstract = "Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.",
journal = "Preprints",
title = "Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation"",
doi = "10.20944/preprints202311.1765.v1"
}

Kozarski, M., Klaus, A., Špirović Trifunović, B., Miletić, S., Lazić, V., Žižak, Ž.,& Vunduk, J.. (2023). Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation". in Preprints.
https://doi.org/10.20944/preprints202311.1765.v1

Kozarski M, Klaus A, Špirović Trifunović B, Miletić S, Lazić V, Žižak Ž, Vunduk J. Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation". in Preprints. 2023;.
doi:10.20944/preprints202311.1765.v1 .

Kozarski, Maja, Klaus, Anita, Špirović Trifunović, Bojana, Miletić, Srđan, Lazić, Vesna, Žižak, Željko, Vunduk, Jovana, "Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation"" in Preprints (2023),
https://doi.org/10.20944/preprints202311.1765.v1 . .

TY  - JOUR
AU  - Kop, Tatjana
AU  - Terzić-Jovanović, Nataša
AU  - Žižak, Željko
AU  - Šolaja, Bogdan
AU  - Milić, Dragana R.
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5356
AB  - Iron salt-promoted reaction of estrone and its derivatives with              meta              -chloroperoxybenzoic acid was developed and epoxyquinols were further transformed. Most compounds showed              in vitro              antiproliferative activity.                      ,                           A new oxidant, containing              m              -chloroperoxybenzoic acid (MCPBA) and an iron salt, was developed and used for oxidation of steroidal phenols to a quinol/epoxyquinol mixture. Reaction was optimized for estrone, by varying initiators (Fe-salts), reaction temperature, time and mode of MCPBA application. A series of five more substrates (17β-estradiol and its hydrophobized derivatives) was subjected to the optimized oxidation, providing corresponding              p              -quinols and 4β,5β-epoxyquinols in good to moderate yields. The obtained epoxyquinols were additionally transformed by oxidation, as well as the acid-catalyzed oxirane opening. In a preliminary study of the antiproliferative activity against human cancer cell lines, all newly synthesized compounds expressed moderate to high activity.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Iron salt-promoted oxidation of steroidal phenols by m -chloroperbenzoic acid: a route to possible antitumor agents
VL  - 12
IS  - 32
SP  - 20649
EP  - 20655
DO  - 10.1039/D2RA03717C
ER  - 

@article{
author = "Kop, Tatjana and Terzić-Jovanović, Nataša and Žižak, Željko and Šolaja, Bogdan and Milić, Dragana R.",
year = "2022",
abstract = "Iron salt-promoted reaction of estrone and its derivatives with              meta              -chloroperoxybenzoic acid was developed and epoxyquinols were further transformed. Most compounds showed              in vitro              antiproliferative activity.                      ,                           A new oxidant, containing              m              -chloroperoxybenzoic acid (MCPBA) and an iron salt, was developed and used for oxidation of steroidal phenols to a quinol/epoxyquinol mixture. Reaction was optimized for estrone, by varying initiators (Fe-salts), reaction temperature, time and mode of MCPBA application. A series of five more substrates (17β-estradiol and its hydrophobized derivatives) was subjected to the optimized oxidation, providing corresponding              p              -quinols and 4β,5β-epoxyquinols in good to moderate yields. The obtained epoxyquinols were additionally transformed by oxidation, as well as the acid-catalyzed oxirane opening. In a preliminary study of the antiproliferative activity against human cancer cell lines, all newly synthesized compounds expressed moderate to high activity.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Iron salt-promoted oxidation of steroidal phenols by m -chloroperbenzoic acid: a route to possible antitumor agents",
volume = "12",
number = "32",
pages = "20649-20655",
doi = "10.1039/D2RA03717C"
}

Kop, T., Terzić-Jovanović, N., Žižak, Ž., Šolaja, B.,& Milić, D. R.. (2022). Iron salt-promoted oxidation of steroidal phenols by m -chloroperbenzoic acid: a route to possible antitumor agents. in RSC Advances
Royal Society of Chemistry., 12(32), 20649-20655.
https://doi.org/10.1039/D2RA03717C

Kop T, Terzić-Jovanović N, Žižak Ž, Šolaja B, Milić DR. Iron salt-promoted oxidation of steroidal phenols by m -chloroperbenzoic acid: a route to possible antitumor agents. in RSC Advances. 2022;12(32):20649-20655.
doi:10.1039/D2RA03717C .

Kop, Tatjana, Terzić-Jovanović, Nataša, Žižak, Željko, Šolaja, Bogdan, Milić, Dragana R., "Iron salt-promoted oxidation of steroidal phenols by m -chloroperbenzoic acid: a route to possible antitumor agents" in RSC Advances, 12, no. 32 (2022):20649-20655,
https://doi.org/10.1039/D2RA03717C . .

TY  - JOUR
AU  - Pantelić, Nebojša Đ.
AU  - Zmejkovski, Bojana
AU  - Žižak, Željko
AU  - Banjac, Nebojša R.
AU  - Božić, Bojan
AU  - Stanojković, Tatjana
AU  - Kaluđerović, Goran N.
PY  - 2019
UR  - https://www.hindawi.com/journals/jchem/2019/2905840/
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2639
AB  - A novel triphenyltin(IV) compound with 1-(4-carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione was synthesized and characterized by IR, NMR spectroscopy, mass spectrometry, and elemental analysis. In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determined against tumor cell lines: human adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human breast cancer (MDA-MB-453), using microculture tetrazolium test (MTT) assay. The results indicate that complex exhibited very high antiproliferative activity against all tested cell lines with IC 50 values in the range of 0.22 to 0.53 µ M. The highest activity organotin(IV) compound expressed against the HeLa cells (IC 50 = 0.22 ± 0.04 µ M). The ligand precursor did not show anticancer activity (IC 50 > 200 µ M). Furthermore, fluorescence microscopy analysis of HeLa cells reveal that organotin(IV) complex induced apoptosis as a mode of cell death, which is consistent with the increase of cells in the sub-G1 phase.
PB  - Hindawi
T2  - Journal of Chemistry
T2  - Journal of Chemistry
T1  - Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione
SP  - 2905840
DO  - 10.1155/2019/2905840
ER  - 

@article{
author = "Pantelić, Nebojša Đ. and Zmejkovski, Bojana and Žižak, Željko and Banjac, Nebojša R. and Božić, Bojan and Stanojković, Tatjana and Kaluđerović, Goran N.",
year = "2019",
abstract = "A novel triphenyltin(IV) compound with 1-(4-carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione was synthesized and characterized by IR, NMR spectroscopy, mass spectrometry, and elemental analysis. In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determined against tumor cell lines: human adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human breast cancer (MDA-MB-453), using microculture tetrazolium test (MTT) assay. The results indicate that complex exhibited very high antiproliferative activity against all tested cell lines with IC 50 values in the range of 0.22 to 0.53 µ M. The highest activity organotin(IV) compound expressed against the HeLa cells (IC 50 = 0.22 ± 0.04 µ M). The ligand precursor did not show anticancer activity (IC 50 > 200 µ M). Furthermore, fluorescence microscopy analysis of HeLa cells reveal that organotin(IV) complex induced apoptosis as a mode of cell death, which is consistent with the increase of cells in the sub-G1 phase.",
publisher = "Hindawi",
journal = "Journal of Chemistry, Journal of Chemistry",
title = "Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione",
pages = "2905840",
doi = "10.1155/2019/2905840"
}

Pantelić, N. Đ., Zmejkovski, B., Žižak, Ž., Banjac, N. R., Božić, B., Stanojković, T.,& Kaluđerović, G. N.. (2019). Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione. in Journal of Chemistry
Hindawi., 2905840.
https://doi.org/10.1155/2019/2905840

Pantelić NĐ, Zmejkovski B, Žižak Ž, Banjac NR, Božić B, Stanojković T, Kaluđerović GN. Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione. in Journal of Chemistry. 2019;:2905840.
doi:10.1155/2019/2905840 .

Pantelić, Nebojša Đ., Zmejkovski, Bojana, Žižak, Željko, Banjac, Nebojša R., Božić, Bojan, Stanojković, Tatjana, Kaluđerović, Goran N., "Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione" in Journal of Chemistry (2019):2905840,
https://doi.org/10.1155/2019/2905840 . .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Žižak, Željko
AU  - Vukovic, Nebojsa
AU  - Šekularac, Gavrilo
AU  - Drmanić, Saša Ž.
AU  - Bugarski, Branko
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1966
AB  - Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.
PB  - Elsevier
T2  - Journal of Functional Foods
T1  - Biological potential of puffballs: A comparative analysis
VL  - 21
SP  - 36
EP  - 49
DO  - 10.1016/j.jff.2015.11.039
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, Jovana and Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Žižak, Željko and Vukovic, Nebojsa and Šekularac, Gavrilo and Drmanić, Saša Ž. and Bugarski, Branko",
year = "2016",
abstract = "Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.",
publisher = "Elsevier",
journal = "Journal of Functional Foods",
title = "Biological potential of puffballs: A comparative analysis",
volume = "21",
pages = "36-49",
doi = "10.1016/j.jff.2015.11.039"
}

Petrović, P., Vunduk, J., Klaus, A., Kozarski, M. S., Nikšić, M., Žižak, Ž., Vukovic, N., Šekularac, G., Drmanić, S. Ž.,& Bugarski, B.. (2016). Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods
Elsevier., 21, 36-49.
https://doi.org/10.1016/j.jff.2015.11.039

Petrović P, Vunduk J, Klaus A, Kozarski MS, Nikšić M, Žižak Ž, Vukovic N, Šekularac G, Drmanić SŽ, Bugarski B. Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods. 2016;21:36-49.
doi:10.1016/j.jff.2015.11.039 .

Petrović, Predrag, Vunduk, Jovana, Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Žižak, Željko, Vukovic, Nebojsa, Šekularac, Gavrilo, Drmanić, Saša Ž., Bugarski, Branko, "Biological potential of puffballs: A comparative analysis" in Journal of Functional Foods, 21 (2016):36-49,
https://doi.org/10.1016/j.jff.2015.11.039 . .

TY  - JOUR
AU  - Popović-Đorđević, Jelena B.
AU  - Klaus, Anita
AU  - Žižak, Željko
AU  - Matić, Ivana Z.
AU  - Drakulić, Branko
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2667
AB  - Antiproliferative and antibacterial activities of nine glutarimide derivatives (1–9) were reported. Cytotoxicity of compounds was tested toward three human cancer cell lines, HeLa, K562 and MDA-MB-453 by MTT assay. Compound 7 (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (IC50 = 9–27 μM). Preliminary screening of antibacterial activity by a disk diffusion method showed that Gram-positive bacteria were more susceptible to the tested compounds than Gram-negative bacteria. Minimum inhibitory concentration (MIC) determined by a broth microdilution method confirmed that compounds 1, 2, 4, 6–8 and 9 inhibited the growth of all tested Gram-positive and some of the Gram-negative bacteria. The best antibacterial potential was achieved with compound 9 (ethyl 4-(1-benzyl-2,6-dioxopiperidin-3-yl)butanoate) against Bacillus cereus (MIC 0.625 mg/mL; 1.97 × 10−3 mol/L). Distinction between more and less active/inactive compounds was assessed from the pharmacophoric patterns obtained by molecular interaction fields.
PB  - Taylor and Francis Ltd
T2  - Journal of Enzyme Inhibition and Medicinal Chemistry
T1  - Antiproliferative and antibacterial activity of some glutarimide derivatives
VL  - 31
SP  - 915
EP  - 923
DO  - 10.3109/14756366.2015.1070844
ER  - 

@article{
author = "Popović-Đorđević, Jelena B. and Klaus, Anita and Žižak, Željko and Matić, Ivana Z. and Drakulić, Branko",
year = "2016",
abstract = "Antiproliferative and antibacterial activities of nine glutarimide derivatives (1–9) were reported. Cytotoxicity of compounds was tested toward three human cancer cell lines, HeLa, K562 and MDA-MB-453 by MTT assay. Compound 7 (2-benzyl-2-azaspiro[5.11]heptadecane-1,3,7-trione), containing 12-membered ketone ring, was found to be the most potent toward all tested cell lines (IC50 = 9–27 μM). Preliminary screening of antibacterial activity by a disk diffusion method showed that Gram-positive bacteria were more susceptible to the tested compounds than Gram-negative bacteria. Minimum inhibitory concentration (MIC) determined by a broth microdilution method confirmed that compounds 1, 2, 4, 6–8 and 9 inhibited the growth of all tested Gram-positive and some of the Gram-negative bacteria. The best antibacterial potential was achieved with compound 9 (ethyl 4-(1-benzyl-2,6-dioxopiperidin-3-yl)butanoate) against Bacillus cereus (MIC 0.625 mg/mL; 1.97 × 10−3 mol/L). Distinction between more and less active/inactive compounds was assessed from the pharmacophoric patterns obtained by molecular interaction fields.",
publisher = "Taylor and Francis Ltd",
journal = "Journal of Enzyme Inhibition and Medicinal Chemistry",
title = "Antiproliferative and antibacterial activity of some glutarimide derivatives",
volume = "31",
pages = "915-923",
doi = "10.3109/14756366.2015.1070844"
}

Popović-Đorđević, J. B., Klaus, A., Žižak, Ž., Matić, I. Z.,& Drakulić, B.. (2016). Antiproliferative and antibacterial activity of some glutarimide derivatives. in Journal of Enzyme Inhibition and Medicinal Chemistry
Taylor and Francis Ltd., 31, 915-923.
https://doi.org/10.3109/14756366.2015.1070844

Popović-Đorđević JB, Klaus A, Žižak Ž, Matić IZ, Drakulić B. Antiproliferative and antibacterial activity of some glutarimide derivatives. in Journal of Enzyme Inhibition and Medicinal Chemistry. 2016;31:915-923.
doi:10.3109/14756366.2015.1070844 .

Popović-Đorđević, Jelena B., Klaus, Anita, Žižak, Željko, Matić, Ivana Z., Drakulić, Branko, "Antiproliferative and antibacterial activity of some glutarimide derivatives" in Journal of Enzyme Inhibition and Medicinal Chemistry, 31 (2016):915-923,
https://doi.org/10.3109/14756366.2015.1070844 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Žižak, Željko
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1666
AB  - The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
VL  - 6
IS  - 6
SP  - 1875
EP  - 1886
DO  - 10.1039/c5fo00312a
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Vunduk, Jovana and Žižak, Željko and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms",
volume = "6",
number = "6",
pages = "1875-1886",
doi = "10.1039/c5fo00312a"
}

Kozarski, M. S., Klaus, A., Vunduk, J., Žižak, Ž., Nikšić, M., Jakovljević, D., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function
Royal Soc Chemistry, Cambridge., 6(6), 1875-1886.
https://doi.org/10.1039/c5fo00312a

Kozarski MS, Klaus A, Vunduk J, Žižak Ž, Nikšić M, Jakovljević D, Vrvić M, Van Griensven LJLD. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function. 2015;6(6):1875-1886.
doi:10.1039/c5fo00312a .

Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Žižak, Željko, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms" in Food & Function, 6, no. 6 (2015):1875-1886,
https://doi.org/10.1039/c5fo00312a . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Vunduk, Jovana
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - Žižak, Željko
AU  - Pavlović, Vladimir B.
AU  - Levic, Steva
AU  - Nikšić, Miomir
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1761
AB  - Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.
PB  - Elsevier
T2  - Food Research International
T1  - Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa
VL  - 67
SP  - 272
EP  - 283
DO  - 10.1016/j.foodres.2014.11.035
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Vunduk, Jovana and Todorović, Nina and Jakovljević, Dragica and Žižak, Željko and Pavlović, Vladimir B. and Levic, Steva and Nikšić, Miomir and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa",
volume = "67",
pages = "272-283",
doi = "10.1016/j.foodres.2014.11.035"
}

Klaus, A., Kozarski, M. S., Vunduk, J., Todorović, N., Jakovljević, D., Žižak, Ž., Pavlović, V. B., Levic, S., Nikšić, M.,& Van Griensven, L. J. L. D.. (2015). Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International
Elsevier., 67, 272-283.
https://doi.org/10.1016/j.foodres.2014.11.035

Klaus A, Kozarski MS, Vunduk J, Todorović N, Jakovljević D, Žižak Ž, Pavlović VB, Levic S, Nikšić M, Van Griensven LJLD. Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International. 2015;67:272-283.
doi:10.1016/j.foodres.2014.11.035 .

Klaus, Anita, Kozarski, Maja S., Vunduk, Jovana, Todorović, Nina, Jakovljević, Dragica, Žižak, Željko, Pavlović, Vladimir B., Levic, Steva, Nikšić, Miomir, Van Griensven, Leo J. L. D., "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa" in Food Research International, 67 (2015):272-283,
https://doi.org/10.1016/j.foodres.2014.11.035 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Aljančić, Ivana
AU  - Žižak, Željko
AU  - Vajs, Vlatka
AU  - Jadranin, Milka
AU  - Milosavljević, Slobodan
AU  - Juranić, Zorica
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1289
AB  - Background: The aim of this research was to determine the intensity and mechanisms of the cytotoxic actions of five extracts isolated from the endemic plant species Helichrysum zivojinii Cernjavski & Soska (family Asteraceae) against specific cancer cell lines. In order to evaluate the sensitivity of normal immunocompetent cells implicated in the antitumor immune response, the cytotoxicity of extracts was also tested against healthy peripheral blood mononuclear cells (PBMC). Methods: The aerial parts of the plants were air-dried, powdered, and successively extracted with solvents of increasing polarity to obtain hexane, dichloromethane, ethyl-acetate, n-butanol and methanol extracts. The cytotoxic activities of the extracts against human cervix adenocarcinoma HeLa, human melanoma Fem-x, human myelogenous leukemia K562, human breast adenocarcinoma MDA-MB-361 cells and PBMC were evaluated by the MTT test. The mode of HeLa cell death was investigated by morphological analysis. Changes in the cell cycle of HeLa cells treated with the extracts were analyzed by flow cytometry. The apoptotic mechanisms induced by the tested extracts were determined using specific caspase inhibitors. Results: The investigated Helichrysum zivojinii extracts exerted selective dose-dependent cytotoxic actions against selected cancer cell lines and healthy immunocompetent PBMC stimulated to proliferate, while the cytotoxic actions exerted on unstimulated PBMC were less pronounced. The tested extracts exhibited considerably stronger cytotoxic activities towards HeLa, Fem-x and K562 cells in comparison to resting and stimulated PBMC. It is worth noting that the cytotoxicity of the extracts was weaker against unstimulated PBMC in comparison to stimulated PBMC. Furthermore, each of the five extracts induced apoptosis in HeLa cells, through the activation of both intrinsic and extrinsic signaling pathways. Conclusion: Extracts obtained from the endemic plant Helichrysum zivojinii may represent an important source of novel potential antitumor agents due to their pronounced and selective cytotoxic actions towards malignant cells.
PB  - Biomed Central Ltd, London
T2  - Bmc Complementary and Alternative Medicine
T1  - In vitro antitumor actions of extracts from endemic plant Helichrysum zivojinii
VL  - 13
DO  - 10.1186/1472-6882-13-36
ER  - 

@article{
author = "Matić, Ivana Z. and Aljančić, Ivana and Žižak, Željko and Vajs, Vlatka and Jadranin, Milka and Milosavljević, Slobodan and Juranić, Zorica",
year = "2013",
abstract = "Background: The aim of this research was to determine the intensity and mechanisms of the cytotoxic actions of five extracts isolated from the endemic plant species Helichrysum zivojinii Cernjavski & Soska (family Asteraceae) against specific cancer cell lines. In order to evaluate the sensitivity of normal immunocompetent cells implicated in the antitumor immune response, the cytotoxicity of extracts was also tested against healthy peripheral blood mononuclear cells (PBMC). Methods: The aerial parts of the plants were air-dried, powdered, and successively extracted with solvents of increasing polarity to obtain hexane, dichloromethane, ethyl-acetate, n-butanol and methanol extracts. The cytotoxic activities of the extracts against human cervix adenocarcinoma HeLa, human melanoma Fem-x, human myelogenous leukemia K562, human breast adenocarcinoma MDA-MB-361 cells and PBMC were evaluated by the MTT test. The mode of HeLa cell death was investigated by morphological analysis. Changes in the cell cycle of HeLa cells treated with the extracts were analyzed by flow cytometry. The apoptotic mechanisms induced by the tested extracts were determined using specific caspase inhibitors. Results: The investigated Helichrysum zivojinii extracts exerted selective dose-dependent cytotoxic actions against selected cancer cell lines and healthy immunocompetent PBMC stimulated to proliferate, while the cytotoxic actions exerted on unstimulated PBMC were less pronounced. The tested extracts exhibited considerably stronger cytotoxic activities towards HeLa, Fem-x and K562 cells in comparison to resting and stimulated PBMC. It is worth noting that the cytotoxicity of the extracts was weaker against unstimulated PBMC in comparison to stimulated PBMC. Furthermore, each of the five extracts induced apoptosis in HeLa cells, through the activation of both intrinsic and extrinsic signaling pathways. Conclusion: Extracts obtained from the endemic plant Helichrysum zivojinii may represent an important source of novel potential antitumor agents due to their pronounced and selective cytotoxic actions towards malignant cells.",
publisher = "Biomed Central Ltd, London",
journal = "Bmc Complementary and Alternative Medicine",
title = "In vitro antitumor actions of extracts from endemic plant Helichrysum zivojinii",
volume = "13",
doi = "10.1186/1472-6882-13-36"
}

Matić, I. Z., Aljančić, I., Žižak, Ž., Vajs, V., Jadranin, M., Milosavljević, S.,& Juranić, Z.. (2013). In vitro antitumor actions of extracts from endemic plant Helichrysum zivojinii. in Bmc Complementary and Alternative Medicine
Biomed Central Ltd, London., 13.
https://doi.org/10.1186/1472-6882-13-36

Matić IZ, Aljančić I, Žižak Ž, Vajs V, Jadranin M, Milosavljević S, Juranić Z. In vitro antitumor actions of extracts from endemic plant Helichrysum zivojinii. in Bmc Complementary and Alternative Medicine. 2013;13.
doi:10.1186/1472-6882-13-36 .

Matić, Ivana Z., Aljančić, Ivana, Žižak, Željko, Vajs, Vlatka, Jadranin, Milka, Milosavljević, Slobodan, Juranić, Zorica, "In vitro antitumor actions of extracts from endemic plant Helichrysum zivojinii" in Bmc Complementary and Alternative Medicine, 13 (2013),
https://doi.org/10.1186/1472-6882-13-36 . .

TY  - CONF
AU  - Žižak, Željko
AU  - Opsenica, Dejan
AU  - Šolaja, Bogdan
AU  - Juranić, Zorica
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1049
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - Investigation of Some Small Heterocyclic Compounds as Potential Antitumor Agents
VL  - 48
DO  - 10.1016/S0959-8049(12)71674-X
ER  - 

@conference{
author = "Žižak, Željko and Opsenica, Dejan and Šolaja, Bogdan and Juranić, Zorica",
year = "2012",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "Investigation of Some Small Heterocyclic Compounds as Potential Antitumor Agents",
volume = "48",
doi = "10.1016/S0959-8049(12)71674-X"
}

Žižak, Ž., Opsenica, D., Šolaja, B.,& Juranić, Z.. (2012). Investigation of Some Small Heterocyclic Compounds as Potential Antitumor Agents. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 48.
https://doi.org/10.1016/S0959-8049(12)71674-X

Žižak Ž, Opsenica D, Šolaja B, Juranić Z. Investigation of Some Small Heterocyclic Compounds as Potential Antitumor Agents. in European Journal of Cancer. 2012;48.
doi:10.1016/S0959-8049(12)71674-X .

Žižak, Željko, Opsenica, Dejan, Šolaja, Bogdan, Juranić, Zorica, "Investigation of Some Small Heterocyclic Compounds as Potential Antitumor Agents" in European Journal of Cancer, 48 (2012),
https://doi.org/10.1016/S0959-8049(12)71674-X . .

TY  - JOUR
AU  - Drakulić, Branko
AU  - Stanojković, Tatjana
AU  - Žižak, Željko
AU  - Dabović, Milan
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2695
AB  - Antiproliferative activity of 27 phenyl-substituted 4-aryl-4-oxo-2-butenoic acids (aroylacrylic acids) toward Human cervix carcinoma (HeLa), Human chronic myelogenous leukemia (K562) and Human colon tumor (LS174) cell lines in vitro are reported. Compounds are active toward all examined cell lines. The most active compounds bear two or three branched alkyl or cycloalkyl substituents on phenyl moiety having potencies in low micromolar ranges. One of most potent derivatives arrests the cell cycle at S phase in HeLa cells. The 3D QSAR study, using molecular interaction fields (MIF) and derived alignment independent descriptors (GRIND-2), rationalize the structural characteristics correlated with potency of compounds. Covalent chemistry, most possibly involved in the mode of action of reported compounds, was quantitatively accounted using frontier molecular orbitals. Pharmacophoric pattern of most potent compounds are used as a template for virtual screening, to find similar ones in database of compounds screened against DTP-NCI 60 tumor cell lines. Potency of obtained hits is well predicted. © 2011 Elsevier Masson SAS. All rights reserved.
PB  - Elsevier
T2  - European Journal of Medicinal Chemistry
T1  - Antiproliferative activity of aroylacrylic acids. Structure-activity study based on molecular interaction fields
VL  - 46
IS  - 8
SP  - 3265
EP  - 3273
DO  - 10.1016/j.ejmech.2011.04.043
ER  - 

@article{
author = "Drakulić, Branko and Stanojković, Tatjana and Žižak, Željko and Dabović, Milan",
year = "2011",
abstract = "Antiproliferative activity of 27 phenyl-substituted 4-aryl-4-oxo-2-butenoic acids (aroylacrylic acids) toward Human cervix carcinoma (HeLa), Human chronic myelogenous leukemia (K562) and Human colon tumor (LS174) cell lines in vitro are reported. Compounds are active toward all examined cell lines. The most active compounds bear two or three branched alkyl or cycloalkyl substituents on phenyl moiety having potencies in low micromolar ranges. One of most potent derivatives arrests the cell cycle at S phase in HeLa cells. The 3D QSAR study, using molecular interaction fields (MIF) and derived alignment independent descriptors (GRIND-2), rationalize the structural characteristics correlated with potency of compounds. Covalent chemistry, most possibly involved in the mode of action of reported compounds, was quantitatively accounted using frontier molecular orbitals. Pharmacophoric pattern of most potent compounds are used as a template for virtual screening, to find similar ones in database of compounds screened against DTP-NCI 60 tumor cell lines. Potency of obtained hits is well predicted. © 2011 Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier",
journal = "European Journal of Medicinal Chemistry",
title = "Antiproliferative activity of aroylacrylic acids. Structure-activity study based on molecular interaction fields",
volume = "46",
number = "8",
pages = "3265-3273",
doi = "10.1016/j.ejmech.2011.04.043"
}

Drakulić, B., Stanojković, T., Žižak, Ž.,& Dabović, M.. (2011). Antiproliferative activity of aroylacrylic acids. Structure-activity study based on molecular interaction fields. in European Journal of Medicinal Chemistry
Elsevier., 46(8), 3265-3273.
https://doi.org/10.1016/j.ejmech.2011.04.043

Drakulić B, Stanojković T, Žižak Ž, Dabović M. Antiproliferative activity of aroylacrylic acids. Structure-activity study based on molecular interaction fields. in European Journal of Medicinal Chemistry. 2011;46(8):3265-3273.
doi:10.1016/j.ejmech.2011.04.043 .

Drakulić, Branko, Stanojković, Tatjana, Žižak, Željko, Dabović, Milan, "Antiproliferative activity of aroylacrylic acids. Structure-activity study based on molecular interaction fields" in European Journal of Medicinal Chemistry, 46, no. 8 (2011):3265-3273,
https://doi.org/10.1016/j.ejmech.2011.04.043 . .

TY  - CONF
AU  - Žižak, Željko
AU  - Juranić, Zorica
AU  - Opsenica, Dejan
AU  - Šolaja, Bogdan
AU  - Besu, I.
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/692
AB  - It was demonstrated that mixed steroidal tetraoxanes inhibit cancer cell proliferation at micromolar level through an apoptotic mechanism. It will be interesting to see if these compounds may possibly induce oxidative stress, which could lead to induction of apoptosis in tumour cells. As tumour cells contain more iron than other normal tissues it is reasonable to suggest that tetraoxanes could react with iron, generating alkoxy radicals or even highly reactive hydroxyl radicals in a Fenton-like reaction. To gain further insight into the mechanism of cell death induced by tetraoxane endoperoxides, we tested production of reactive oxygen species (ROS) and level of activation of caspase 3 in tumour cells treated with several newly synthesized tetraoxanes.
PB  - Oxford : Pergamon-Elsevier Science Ltd
C3  - European Journal of Cancer Supplements
T1  - Tetraoxanes induced ROS production and activation of caspase 3 in HeLa cells
VL  - 8
IS  - 5
SP  - 131
EP  - 131
DO  - 10.1016/S1359-6349(10)71313-6
ER  - 

@conference{
author = "Žižak, Željko and Juranić, Zorica and Opsenica, Dejan and Šolaja, Bogdan and Besu, I.",
year = "2010",
abstract = "It was demonstrated that mixed steroidal tetraoxanes inhibit cancer cell proliferation at micromolar level through an apoptotic mechanism. It will be interesting to see if these compounds may possibly induce oxidative stress, which could lead to induction of apoptosis in tumour cells. As tumour cells contain more iron than other normal tissues it is reasonable to suggest that tetraoxanes could react with iron, generating alkoxy radicals or even highly reactive hydroxyl radicals in a Fenton-like reaction. To gain further insight into the mechanism of cell death induced by tetraoxane endoperoxides, we tested production of reactive oxygen species (ROS) and level of activation of caspase 3 in tumour cells treated with several newly synthesized tetraoxanes.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "European Journal of Cancer Supplements",
title = "Tetraoxanes induced ROS production and activation of caspase 3 in HeLa cells",
volume = "8",
number = "5",
pages = "131-131",
doi = "10.1016/S1359-6349(10)71313-6"
}

Žižak, Ž., Juranić, Z., Opsenica, D., Šolaja, B.,& Besu, I.. (2010). Tetraoxanes induced ROS production and activation of caspase 3 in HeLa cells. in European Journal of Cancer Supplements
Oxford : Pergamon-Elsevier Science Ltd., 8(5), 131-131.
https://doi.org/10.1016/S1359-6349(10)71313-6

Žižak Ž, Juranić Z, Opsenica D, Šolaja B, Besu I. Tetraoxanes induced ROS production and activation of caspase 3 in HeLa cells. in European Journal of Cancer Supplements. 2010;8(5):131-131.
doi:10.1016/S1359-6349(10)71313-6 .

Žižak, Željko, Juranić, Zorica, Opsenica, Dejan, Šolaja, Bogdan, Besu, I., "Tetraoxanes induced ROS production and activation of caspase 3 in HeLa cells" in European Journal of Cancer Supplements, 8, no. 5 (2010):131-131,
https://doi.org/10.1016/S1359-6349(10)71313-6 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Žižak, Željko
AU  - Simonović, Mladen
AU  - Simonović, Branislav
AU  - Gođevac, Dejan
AU  - Savikin, Katarina
AU  - Juranić, Zorica
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/697
AB  - Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.
PB  - Mary Ann Liebert Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells
VL  - 13
IS  - 4
SP  - 851
EP  - 862
DO  - 10.1089/jmf.2009.0193
ER  - 

@article{
author = "Matić, Ivana Z. and Žižak, Željko and Simonović, Mladen and Simonović, Branislav and Gođevac, Dejan and Savikin, Katarina and Juranić, Zorica",
year = "2010",
abstract = "Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.",
publisher = "Mary Ann Liebert Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells",
volume = "13",
number = "4",
pages = "851-862",
doi = "10.1089/jmf.2009.0193"
}

Matić, I. Z., Žižak, Ž., Simonović, M., Simonović, B., Gođevac, D., Savikin, K.,& Juranić, Z.. (2010). Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells. in Journal of Medicinal Food
Mary Ann Liebert Inc, New Rochelle., 13(4), 851-862.
https://doi.org/10.1089/jmf.2009.0193

Matić IZ, Žižak Ž, Simonović M, Simonović B, Gođevac D, Savikin K, Juranić Z. Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells. in Journal of Medicinal Food. 2010;13(4):851-862.
doi:10.1089/jmf.2009.0193 .

Matić, Ivana Z., Žižak, Željko, Simonović, Mladen, Simonović, Branislav, Gođevac, Dejan, Savikin, Katarina, Juranić, Zorica, "Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells" in Journal of Medicinal Food, 13, no. 4 (2010):851-862,
https://doi.org/10.1089/jmf.2009.0193 . .

TY  - JOUR
AU  - Cvijetić, Ilija
AU  - Žižak, Željko
AU  - Stanojković, Tatjana
AU  - Juranić, Zorica
AU  - Terzić-Jovanović, Nataša
AU  - Opsenica, Igor
AU  - Opsenica, Dejan
AU  - Juranić, Ivan
AU  - Drakulić, Branko
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/716
AB  - An alignment-free 3D QSAR study on antiproliferative activity of the thirty-three 1,2,4,5-tetraoxane derivatives toward two human dedifferentiated cell lines was reported. GRIND methodology, where descriptors are derived from GRID molecular interaction fields (MIF), were used It was found that pharmacophoric pattern attributed to the most potent derivatives Include amido NH of the primary or secondary amide, and the acetoxy fragments at positions 7 and 12 of steroid core which are, along with the tetraoxane ring, common for all studied compounds. Independently, simple multiple regression model obtained by using the whole-molecular properties, confirmed that the hydrophobicity and the H-bond donor properties are the main parameters influencing potency of compounds toward human cervix carcinoma (HeLa) and human malignant melanoma (FemX) cell lines Corollary, similar structural motifs are found to be Important for the potency toward both examined cell lines.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - An alignment independent 3D QSAR study of the antiproliferative activity of 1,2,4,5-tetraoxanes
VL  - 45
IS  - 10
SP  - 4570
EP  - 4577
DO  - 10.1016/j.ejmech.2010.07.019
ER  - 

@article{
author = "Cvijetić, Ilija and Žižak, Željko and Stanojković, Tatjana and Juranić, Zorica and Terzić-Jovanović, Nataša and Opsenica, Igor and Opsenica, Dejan and Juranić, Ivan and Drakulić, Branko",
year = "2010",
abstract = "An alignment-free 3D QSAR study on antiproliferative activity of the thirty-three 1,2,4,5-tetraoxane derivatives toward two human dedifferentiated cell lines was reported. GRIND methodology, where descriptors are derived from GRID molecular interaction fields (MIF), were used It was found that pharmacophoric pattern attributed to the most potent derivatives Include amido NH of the primary or secondary amide, and the acetoxy fragments at positions 7 and 12 of steroid core which are, along with the tetraoxane ring, common for all studied compounds. Independently, simple multiple regression model obtained by using the whole-molecular properties, confirmed that the hydrophobicity and the H-bond donor properties are the main parameters influencing potency of compounds toward human cervix carcinoma (HeLa) and human malignant melanoma (FemX) cell lines Corollary, similar structural motifs are found to be Important for the potency toward both examined cell lines.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "An alignment independent 3D QSAR study of the antiproliferative activity of 1,2,4,5-tetraoxanes",
volume = "45",
number = "10",
pages = "4570-4577",
doi = "10.1016/j.ejmech.2010.07.019"
}

Cvijetić, I., Žižak, Ž., Stanojković, T., Juranić, Z., Terzić-Jovanović, N., Opsenica, I., Opsenica, D., Juranić, I.,& Drakulić, B.. (2010). An alignment independent 3D QSAR study of the antiproliferative activity of 1,2,4,5-tetraoxanes. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 45(10), 4570-4577.
https://doi.org/10.1016/j.ejmech.2010.07.019

Cvijetić I, Žižak Ž, Stanojković T, Juranić Z, Terzić-Jovanović N, Opsenica I, Opsenica D, Juranić I, Drakulić B. An alignment independent 3D QSAR study of the antiproliferative activity of 1,2,4,5-tetraoxanes. in European Journal of Medicinal Chemistry. 2010;45(10):4570-4577.
doi:10.1016/j.ejmech.2010.07.019 .

Cvijetić, Ilija, Žižak, Željko, Stanojković, Tatjana, Juranić, Zorica, Terzić-Jovanović, Nataša, Opsenica, Igor, Opsenica, Dejan, Juranić, Ivan, Drakulić, Branko, "An alignment independent 3D QSAR study of the antiproliferative activity of 1,2,4,5-tetraoxanes" in European Journal of Medicinal Chemistry, 45, no. 10 (2010):4570-4577,
https://doi.org/10.1016/j.ejmech.2010.07.019 . .

TY  - JOUR
AU  - Vitnik, Vesna
AU  - Ivanović, Milovan D.
AU  - Vitnik, Željko
AU  - Đorđević, Jelena B.
AU  - Žižak, Željko
AU  - Juranić, Zorica
AU  - Juranić, Ivan
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/576
AB  - Phase-transfer-catalyzed (PTC) reactions of ketones with bromoform and aqueous lithium hydroxide in alcoholic solvent result in the formation of ,-unsaturated carboxylic acids. The reaction was performed at room temperature for 24h. The corresponding conjugated acids were obtained from cyclic or aromatic ketones, whereas bromo acids were obtained from 4-oxo-piperidine-1- carboxylic acid ethyl ester (13) and 4-oxo-piperidine-1-carboxylic acid tert-butyl ester (14).
T2  - Synthetic Communications
T1  - One-step conversion of ketones to conjugated acids using bromoform
VL  - 39
IS  - 8
SP  - 1457
EP  - 1471
DO  - 10.1080/00397910802531955
ER  - 

@article{
author = "Vitnik, Vesna and Ivanović, Milovan D. and Vitnik, Željko and Đorđević, Jelena B. and Žižak, Željko and Juranić, Zorica and Juranić, Ivan",
year = "2009",
abstract = "Phase-transfer-catalyzed (PTC) reactions of ketones with bromoform and aqueous lithium hydroxide in alcoholic solvent result in the formation of ,-unsaturated carboxylic acids. The reaction was performed at room temperature for 24h. The corresponding conjugated acids were obtained from cyclic or aromatic ketones, whereas bromo acids were obtained from 4-oxo-piperidine-1- carboxylic acid ethyl ester (13) and 4-oxo-piperidine-1-carboxylic acid tert-butyl ester (14).",
journal = "Synthetic Communications",
title = "One-step conversion of ketones to conjugated acids using bromoform",
volume = "39",
number = "8",
pages = "1457-1471",
doi = "10.1080/00397910802531955"
}

Vitnik, V., Ivanović, M. D., Vitnik, Ž., Đorđević, J. B., Žižak, Ž., Juranić, Z.,& Juranić, I.. (2009). One-step conversion of ketones to conjugated acids using bromoform. in Synthetic Communications, 39(8), 1457-1471.
https://doi.org/10.1080/00397910802531955

Vitnik V, Ivanović MD, Vitnik Ž, Đorđević JB, Žižak Ž, Juranić Z, Juranić I. One-step conversion of ketones to conjugated acids using bromoform. in Synthetic Communications. 2009;39(8):1457-1471.
doi:10.1080/00397910802531955 .

Vitnik, Vesna, Ivanović, Milovan D., Vitnik, Željko, Đorđević, Jelena B., Žižak, Željko, Juranić, Zorica, Juranić, Ivan, "One-step conversion of ketones to conjugated acids using bromoform" in Synthetic Communications, 39, no. 8 (2009):1457-1471,
https://doi.org/10.1080/00397910802531955 . .

TY  - JOUR
AU  - Zmejkovski, Bojana
AU  - Kaluđerović, Goran N.
AU  - Gómez-Ruiz, S.
AU  - Žižak, Željko
AU  - Steinborn, D.
AU  - Schmidt, H.
AU  - Paschke, Reinhard
AU  - Juranić, Zorica
AU  - Sabo, Tibor
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/578
AB  - New R2eddip-type esters (R = cyclopentyl, L3·2HCl 1.5H2O; cyclohexyl, L4·2HCl·H2O) and corresponding palladium(II) complexes, [PdCl2L3] (3) and [PdCl2L4]·H2O (4), as well as [PdCl2L2] (2; L2 diisobutyl ester of eddip) were synthesized and characterized by IR, 1H and 13C NMR spectroscopies and elemental analysis. The crystal structure of L3·2HCl·2CHCl3 was resolved and is given herein. The NMR spectroscopy confirmed the presence of two isomers (from three possible) for each palladium(II) complex. DFT calculations support the formation of two diastereoisomers. In addition, antitumoral investigations were performed and these investigations also included the diisopropyl ester of eddip (L1) and corresponding palladium(II) complex, [PdCl2L1] (1). In vitro antiproliferative activity was determined against several tumor cell lines HeLa, Fem-x, K562 and rested and stimulated normal immunocompetent cells (human peripheral blood mononuclear cells -PBMCs) using MTT test.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Palladium(II) complexes with R2edda-derived ligands. Part II. Synthesis, characterization and in vitro antitumoral studies of R2eddip esters and palladium(II) complexes
VL  - 44
IS  - 9
SP  - 3452
EP  - 3458
DO  - 10.1016/j.ejmech.2009.02.002
ER  - 

@article{
author = "Zmejkovski, Bojana and Kaluđerović, Goran N. and Gómez-Ruiz, S. and Žižak, Željko and Steinborn, D. and Schmidt, H. and Paschke, Reinhard and Juranić, Zorica and Sabo, Tibor",
year = "2009",
abstract = "New R2eddip-type esters (R = cyclopentyl, L3·2HCl 1.5H2O; cyclohexyl, L4·2HCl·H2O) and corresponding palladium(II) complexes, [PdCl2L3] (3) and [PdCl2L4]·H2O (4), as well as [PdCl2L2] (2; L2 diisobutyl ester of eddip) were synthesized and characterized by IR, 1H and 13C NMR spectroscopies and elemental analysis. The crystal structure of L3·2HCl·2CHCl3 was resolved and is given herein. The NMR spectroscopy confirmed the presence of two isomers (from three possible) for each palladium(II) complex. DFT calculations support the formation of two diastereoisomers. In addition, antitumoral investigations were performed and these investigations also included the diisopropyl ester of eddip (L1) and corresponding palladium(II) complex, [PdCl2L1] (1). In vitro antiproliferative activity was determined against several tumor cell lines HeLa, Fem-x, K562 and rested and stimulated normal immunocompetent cells (human peripheral blood mononuclear cells -PBMCs) using MTT test.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Palladium(II) complexes with R2edda-derived ligands. Part II. Synthesis, characterization and in vitro antitumoral studies of R2eddip esters and palladium(II) complexes",
volume = "44",
number = "9",
pages = "3452-3458",
doi = "10.1016/j.ejmech.2009.02.002"
}

Zmejkovski, B., Kaluđerović, G. N., Gómez-Ruiz, S., Žižak, Ž., Steinborn, D., Schmidt, H., Paschke, R., Juranić, Z.,& Sabo, T.. (2009). Palladium(II) complexes with R2edda-derived ligands. Part II. Synthesis, characterization and in vitro antitumoral studies of R2eddip esters and palladium(II) complexes. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 44(9), 3452-3458.
https://doi.org/10.1016/j.ejmech.2009.02.002

Zmejkovski B, Kaluđerović GN, Gómez-Ruiz S, Žižak Ž, Steinborn D, Schmidt H, Paschke R, Juranić Z, Sabo T. Palladium(II) complexes with R2edda-derived ligands. Part II. Synthesis, characterization and in vitro antitumoral studies of R2eddip esters and palladium(II) complexes. in European Journal of Medicinal Chemistry. 2009;44(9):3452-3458.
doi:10.1016/j.ejmech.2009.02.002 .

Zmejkovski, Bojana, Kaluđerović, Goran N., Gómez-Ruiz, S., Žižak, Željko, Steinborn, D., Schmidt, H., Paschke, Reinhard, Juranić, Zorica, Sabo, Tibor, "Palladium(II) complexes with R2edda-derived ligands. Part II. Synthesis, characterization and in vitro antitumoral studies of R2eddip esters and palladium(II) complexes" in European Journal of Medicinal Chemistry, 44, no. 9 (2009):3452-3458,
https://doi.org/10.1016/j.ejmech.2009.02.002 . .

TY  - JOUR
AU  - Milić, Dragana
AU  - Kop, Tatjana
AU  - Csanadi, J.
AU  - Juranić, Zorica
AU  - Žižak, Željko
AU  - Gašić, Miroslav J.
AU  - Šolaja, Bogdan
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/618
AB  - A simple approach to a stable steroidal estrone derived A,B-spiro system is reported. Treatment of estrone derived A-ring diepoxyalcohol with the Ac2O-TMSOTf system at the ambient temperature led to acetylation, while at the reflux temperature the acid-catalysed rearrangement took place affording the spiro-compound. Results of extensive in vitro and in vivo anticancer tests on the diepoxide, as well as preliminary data on the antiproliferative activity of the spiro-product against three cancer cell lines, are also presented.
PB  - Elsevier Science Inc, New York
T2  - Steroids
T1  - Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system
VL  - 74
IS  - 12
SP  - 890
EP  - 895
DO  - 10.1016/j.steroids.2009.06.002
ER  - 

@article{
author = "Milić, Dragana and Kop, Tatjana and Csanadi, J. and Juranić, Zorica and Žižak, Željko and Gašić, Miroslav J. and Šolaja, Bogdan",
year = "2009",
abstract = "A simple approach to a stable steroidal estrone derived A,B-spiro system is reported. Treatment of estrone derived A-ring diepoxyalcohol with the Ac2O-TMSOTf system at the ambient temperature led to acetylation, while at the reflux temperature the acid-catalysed rearrangement took place affording the spiro-compound. Results of extensive in vitro and in vivo anticancer tests on the diepoxide, as well as preliminary data on the antiproliferative activity of the spiro-product against three cancer cell lines, are also presented.",
publisher = "Elsevier Science Inc, New York",
journal = "Steroids",
title = "Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system",
volume = "74",
number = "12",
pages = "890-895",
doi = "10.1016/j.steroids.2009.06.002"
}

Milić, D., Kop, T., Csanadi, J., Juranić, Z., Žižak, Ž., Gašić, M. J.,& Šolaja, B.. (2009). Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system. in Steroids
Elsevier Science Inc, New York., 74(12), 890-895.
https://doi.org/10.1016/j.steroids.2009.06.002

Milić D, Kop T, Csanadi J, Juranić Z, Žižak Ž, Gašić MJ, Šolaja B. Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system. in Steroids. 2009;74(12):890-895.
doi:10.1016/j.steroids.2009.06.002 .

Milić, Dragana, Kop, Tatjana, Csanadi, J., Juranić, Zorica, Žižak, Željko, Gašić, Miroslav J., Šolaja, Bogdan, "Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system" in Steroids, 74, no. 12 (2009):890-895,
https://doi.org/10.1016/j.steroids.2009.06.002 . .

TY  - JOUR
AU  - Žižak, Željko
AU  - Juranić, Zorica
AU  - Opsenica, Dejan
AU  - Šolaja, Bogdan
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/624
AB  - In this study we investigated the antiproliferative activity of six mixed steroidal tetraoxanes against various tumor cell lines, the toxicity against normal peripheral blood mononuclear cells (PBMC), and the mode of HeLa cell death induced by these compounds. Investigated tetraoxanes exerted a dose dependent antiproliferative action at micromolar concentrations toward target tumor cell lines. Treatment of HeLa cells for 24 h with all tetraoxanes induced apoptosis, as confirmed by morphological analysis and by the appearance of a typical ladder pattern in the DNA fragmentation assay.
T2  - Investigational New Drugs
T1  - Mixed steroidal tetraoxanes induce apoptotic cell death in tumor cells
VL  - 27
IS  - 5
SP  - 432
EP  - 439
DO  - 10.1007/s10637-008-9197-1
ER  - 

@article{
author = "Žižak, Željko and Juranić, Zorica and Opsenica, Dejan and Šolaja, Bogdan",
year = "2009",
abstract = "In this study we investigated the antiproliferative activity of six mixed steroidal tetraoxanes against various tumor cell lines, the toxicity against normal peripheral blood mononuclear cells (PBMC), and the mode of HeLa cell death induced by these compounds. Investigated tetraoxanes exerted a dose dependent antiproliferative action at micromolar concentrations toward target tumor cell lines. Treatment of HeLa cells for 24 h with all tetraoxanes induced apoptosis, as confirmed by morphological analysis and by the appearance of a typical ladder pattern in the DNA fragmentation assay.",
journal = "Investigational New Drugs",
title = "Mixed steroidal tetraoxanes induce apoptotic cell death in tumor cells",
volume = "27",
number = "5",
pages = "432-439",
doi = "10.1007/s10637-008-9197-1"
}

Žižak, Ž., Juranić, Z., Opsenica, D.,& Šolaja, B.. (2009). Mixed steroidal tetraoxanes induce apoptotic cell death in tumor cells. in Investigational New Drugs, 27(5), 432-439.
https://doi.org/10.1007/s10637-008-9197-1

Žižak Ž, Juranić Z, Opsenica D, Šolaja B. Mixed steroidal tetraoxanes induce apoptotic cell death in tumor cells. in Investigational New Drugs. 2009;27(5):432-439.
doi:10.1007/s10637-008-9197-1 .

Žižak, Željko, Juranić, Zorica, Opsenica, Dejan, Šolaja, Bogdan, "Mixed steroidal tetraoxanes induce apoptotic cell death in tumor cells" in Investigational New Drugs, 27, no. 5 (2009):432-439,
https://doi.org/10.1007/s10637-008-9197-1 . .

TY  - JOUR
AU  - Pérez‐Quintanilla, Damian
AU  - Gómez‐Ruiz, Santiago
AU  - Žižak, Željko
AU  - Sierra, Isabel
AU  - Prashar, Sanjiv
AU  - del Hierro, Isabel
AU  - Fajardo, Mariano
AU  - Juranić, Zorica
AU  - Kaluđerović, Goran N.
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4124
AB  - Dehydroxylated MCM-41 and SBA-15 surfaces were modified by the grafting of two different titanocene complexes ([Ti(Tη5-C5H 4Me)2Cl2] and [Ti(Me2Si(η 5-C5Me4) (η5-C5H 4)}Cl2]) to give new materials, which have been characterized by powder X-ray diffraction, X-ray fluorescence, nitrogen gas sorption, MAS-NMR spectroscopy, thermogravimetry, SEM, and TEM. The toxicity of the resulting materials toward human adenocarcinoma HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x, and normal immunocompetent cells, such as peripheral blood mononuclear cells PBMC has been studied. Estimation of the number of particles per gram of material led to the calculation of O 50 values for these samples, which is the number of particles required to inhibit normal cell growth by 50%. In addition, M50 values (quantity of material needed to inhibit normal cell growth by 50%) of the studied surfaces is also reported. Nonfunctionalized MCM-41 and SBA-15 did not show notable antiproliferative activity, whereas functionalization of these materials with different titanocene based anticancer drugs led to very promising antitumoral activity. The best Q50 values correspond to titanocene functionalized MCM-41 surfaces (MCM-41/[Ti(η5C5H 4Me)2Cl2] (1) and MCM-41/[TiIMe 2Si(Tf-C5Me4)(Tf-C5H 4)(Cl2] (2)) with Q50 values between 3.8 ± 0.6 x 108 and 24.5 ±3.0 x 108 particles. Titanocene functionalized SBA-15 surfaces (SBA-15/[Ti(η5-C 5H4Me)2Cl2] (3) and SBA-15/[Ti{Me2Si(η5-C5Me 4)(η5C5H4)}Cl2] (4)) gave higher Q50 values, showing lower activity from 73.2 ± 9.9 x 108 to 362±7×l08 particles. The best response of the studied materials in terms of M50 values was observed against Fem-x (309 ± 42 g for 4) and K562 (338±18 g for 2), whereas moderate activities were observed in HeLa cells (from 508±63g of 2 to 912 ± 10g of 1). In addition, the analyzed surfaces presented only marginal activity against unstimulated and stimulated PBMC, showing a slight selectivity on human cancer cells. Comparison of the in vitro cytotoxicity in solution of the titanocene complexes/[Ti(η5-C5H 4Me)2Cl2] (3) and SBA-15/[Ti{Me 2Si(η5-C5Me4) (η5C5H4)}Cl2] (4)) gave higher Q50 va and the corresponding titanocene functionalized materials is also described.
PB  - Wiley
T2  - Chemistry a European Journal
T1  - A New Generation of Anticancer Drugs: Mesoporous Materials Modified with Titanocene Complexes
VL  - 15
IS  - 22
SP  - 5588
EP  - 5597
DO  - 10.1002/chem.200900151
ER  - 

@article{
author = "Pérez‐Quintanilla, Damian and Gómez‐Ruiz, Santiago and Žižak, Željko and Sierra, Isabel and Prashar, Sanjiv and del Hierro, Isabel and Fajardo, Mariano and Juranić, Zorica and Kaluđerović, Goran N.",
year = "2009",
abstract = "Dehydroxylated MCM-41 and SBA-15 surfaces were modified by the grafting of two different titanocene complexes ([Ti(Tη5-C5H 4Me)2Cl2] and [Ti(Me2Si(η 5-C5Me4) (η5-C5H 4)}Cl2]) to give new materials, which have been characterized by powder X-ray diffraction, X-ray fluorescence, nitrogen gas sorption, MAS-NMR spectroscopy, thermogravimetry, SEM, and TEM. The toxicity of the resulting materials toward human adenocarcinoma HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x, and normal immunocompetent cells, such as peripheral blood mononuclear cells PBMC has been studied. Estimation of the number of particles per gram of material led to the calculation of O 50 values for these samples, which is the number of particles required to inhibit normal cell growth by 50%. In addition, M50 values (quantity of material needed to inhibit normal cell growth by 50%) of the studied surfaces is also reported. Nonfunctionalized MCM-41 and SBA-15 did not show notable antiproliferative activity, whereas functionalization of these materials with different titanocene based anticancer drugs led to very promising antitumoral activity. The best Q50 values correspond to titanocene functionalized MCM-41 surfaces (MCM-41/[Ti(η5C5H 4Me)2Cl2] (1) and MCM-41/[TiIMe 2Si(Tf-C5Me4)(Tf-C5H 4)(Cl2] (2)) with Q50 values between 3.8 ± 0.6 x 108 and 24.5 ±3.0 x 108 particles. Titanocene functionalized SBA-15 surfaces (SBA-15/[Ti(η5-C 5H4Me)2Cl2] (3) and SBA-15/[Ti{Me2Si(η5-C5Me 4)(η5C5H4)}Cl2] (4)) gave higher Q50 values, showing lower activity from 73.2 ± 9.9 x 108 to 362±7×l08 particles. The best response of the studied materials in terms of M50 values was observed against Fem-x (309 ± 42 g for 4) and K562 (338±18 g for 2), whereas moderate activities were observed in HeLa cells (from 508±63g of 2 to 912 ± 10g of 1). In addition, the analyzed surfaces presented only marginal activity against unstimulated and stimulated PBMC, showing a slight selectivity on human cancer cells. Comparison of the in vitro cytotoxicity in solution of the titanocene complexes/[Ti(η5-C5H 4Me)2Cl2] (3) and SBA-15/[Ti{Me 2Si(η5-C5Me4) (η5C5H4)}Cl2] (4)) gave higher Q50 va and the corresponding titanocene functionalized materials is also described.",
publisher = "Wiley",
journal = "Chemistry a European Journal",
title = "A New Generation of Anticancer Drugs: Mesoporous Materials Modified with Titanocene Complexes",
volume = "15",
number = "22",
pages = "5588-5597",
doi = "10.1002/chem.200900151"
}

Pérez‐Quintanilla, D., Gómez‐Ruiz, S., Žižak, Ž., Sierra, I., Prashar, S., del Hierro, I., Fajardo, M., Juranić, Z.,& Kaluđerović, G. N.. (2009). A New Generation of Anticancer Drugs: Mesoporous Materials Modified with Titanocene Complexes. in Chemistry a European Journal
Wiley., 15(22), 5588-5597.
https://doi.org/10.1002/chem.200900151

Pérez‐Quintanilla D, Gómez‐Ruiz S, Žižak Ž, Sierra I, Prashar S, del Hierro I, Fajardo M, Juranić Z, Kaluđerović GN. A New Generation of Anticancer Drugs: Mesoporous Materials Modified with Titanocene Complexes. in Chemistry a European Journal. 2009;15(22):5588-5597.
doi:10.1002/chem.200900151 .

Pérez‐Quintanilla, Damian, Gómez‐Ruiz, Santiago, Žižak, Željko, Sierra, Isabel, Prashar, Sanjiv, del Hierro, Isabel, Fajardo, Mariano, Juranić, Zorica, Kaluđerović, Goran N., "A New Generation of Anticancer Drugs: Mesoporous Materials Modified with Titanocene Complexes" in Chemistry a European Journal, 15, no. 22 (2009):5588-5597,
https://doi.org/10.1002/chem.200900151 . .

TY  - JOUR
AU  - Gómez-Ruiz, Santiago
AU  - Kaluđerović, Goran N.
AU  - Prashar, Sanjiv
AU  - Hey-Hawkins, Evamarie
AU  - Erić, Aleksandra
AU  - Žižak, Željko
AU  - Juranić, Zorica
PY  - 2008
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4125
AB  - The reaction of 3-methoxyphenylacetic acid (3-MPAH), 4-methoxyphenylacetic acid (4-MPAH), 2,5-
dimethyl-3-furoic acid (DMFUH) or 1,4-benzodioxane-6-carboxylic acid (BZDOH) with triphenyltin(IV)
chloride (1:1) or diphenyltin(IV) dichloride (2:1) in the presence of triethylamine yielded the compounds
[SnPh3(3-MPA)] (1), [SnPh3(4-MPA)] (2), [SnPh3(DMFU)] (3), [SnPh3(BZDO)] (4), [SnPh2(3-MPA)2] (5),
[SnPh2(4-MPA)2] (6), [SnPh2(DMFU)2] (7) and [SnPh2(BZDO)2] (8), respectively. The tetranuclear complex
[{Me2(DMFU)SnOSn(DMFU)Me2}2] (9) was prepared by the reaction of dimethyltin(IV) oxide and 2,5-
dimethyl-3-furoic acid (DMFUH). The molecular structures of 3, 4 and 9, were determined by X-ray diffraction
studies. The cytotoxic activity of the carboxylic acids (3-MPAH, 4-MPAH, BZDOH and DMFUH)
and di (5–8) and triphenyltin(IV) complexes (2–4) was tested against tumor cell lines human adenocarcinoma
HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x and normal
immunocompetent cells, peripheral blood mononuclear cells PBMC. Triphenyltin(IV) complexes show
higher activities than the diphenyltin(IV) derivatives. The most active compound is [SnPh3(DMFU)] (3)
with IC50 value of 0.15  } 0.01, 0.051  } 0.004, 0.074  } 0.004, 0.20  } 0.01, 0.15  } 0.02 on HeLa, K562, Femx,
rested and stimulated PBMC, respectively, while the most selective are [SnPh2(3-MPA)2] (5),
[SnPh2(DMFU)2] (7) and [SnPh2(BZDO)2] (8). Compounds 3, 5, 7 and 8 present higher activities than cisplatin
in all the tested cells and relative high selectivity especially on K562 cells.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Study of the cytotoxic activity of di and triphenyltin(IV) carboxylate complexes
VL  - 102
IS  - 12
SP  - 2087
EP  - 2096
DO  - 10.1016/j.jinorgbio.2008.07.009
ER  - 

@article{
author = "Gómez-Ruiz, Santiago and Kaluđerović, Goran N. and Prashar, Sanjiv and Hey-Hawkins, Evamarie and Erić, Aleksandra and Žižak, Željko and Juranić, Zorica",
year = "2008",
abstract = "The reaction of 3-methoxyphenylacetic acid (3-MPAH), 4-methoxyphenylacetic acid (4-MPAH), 2,5-
dimethyl-3-furoic acid (DMFUH) or 1,4-benzodioxane-6-carboxylic acid (BZDOH) with triphenyltin(IV)
chloride (1:1) or diphenyltin(IV) dichloride (2:1) in the presence of triethylamine yielded the compounds
[SnPh3(3-MPA)] (1), [SnPh3(4-MPA)] (2), [SnPh3(DMFU)] (3), [SnPh3(BZDO)] (4), [SnPh2(3-MPA)2] (5),
[SnPh2(4-MPA)2] (6), [SnPh2(DMFU)2] (7) and [SnPh2(BZDO)2] (8), respectively. The tetranuclear complex
[{Me2(DMFU)SnOSn(DMFU)Me2}2] (9) was prepared by the reaction of dimethyltin(IV) oxide and 2,5-
dimethyl-3-furoic acid (DMFUH). The molecular structures of 3, 4 and 9, were determined by X-ray diffraction
studies. The cytotoxic activity of the carboxylic acids (3-MPAH, 4-MPAH, BZDOH and DMFUH)
and di (5–8) and triphenyltin(IV) complexes (2–4) was tested against tumor cell lines human adenocarcinoma
HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x and normal
immunocompetent cells, peripheral blood mononuclear cells PBMC. Triphenyltin(IV) complexes show
higher activities than the diphenyltin(IV) derivatives. The most active compound is [SnPh3(DMFU)] (3)
with IC50 value of 0.15  } 0.01, 0.051  } 0.004, 0.074  } 0.004, 0.20  } 0.01, 0.15  } 0.02 on HeLa, K562, Femx,
rested and stimulated PBMC, respectively, while the most selective are [SnPh2(3-MPA)2] (5),
[SnPh2(DMFU)2] (7) and [SnPh2(BZDO)2] (8). Compounds 3, 5, 7 and 8 present higher activities than cisplatin
in all the tested cells and relative high selectivity especially on K562 cells.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Study of the cytotoxic activity of di and triphenyltin(IV) carboxylate complexes",
volume = "102",
number = "12",
pages = "2087-2096",
doi = "10.1016/j.jinorgbio.2008.07.009"
}

Gómez-Ruiz, S., Kaluđerović, G. N., Prashar, S., Hey-Hawkins, E., Erić, A., Žižak, Ž.,& Juranić, Z.. (2008). Study of the cytotoxic activity of di and triphenyltin(IV) carboxylate complexes. in Journal of Inorganic Biochemistry
Elsevier., 102(12), 2087-2096.
https://doi.org/10.1016/j.jinorgbio.2008.07.009

Gómez-Ruiz S, Kaluđerović GN, Prashar S, Hey-Hawkins E, Erić A, Žižak Ž, Juranić Z. Study of the cytotoxic activity of di and triphenyltin(IV) carboxylate complexes. in Journal of Inorganic Biochemistry. 2008;102(12):2087-2096.
doi:10.1016/j.jinorgbio.2008.07.009 .

Gómez-Ruiz, Santiago, Kaluđerović, Goran N., Prashar, Sanjiv, Hey-Hawkins, Evamarie, Erić, Aleksandra, Žižak, Željko, Juranić, Zorica, "Study of the cytotoxic activity of di and triphenyltin(IV) carboxylate complexes" in Journal of Inorganic Biochemistry, 102, no. 12 (2008):2087-2096,
https://doi.org/10.1016/j.jinorgbio.2008.07.009 . .

TY  - JOUR
AU  - Gomez-Ruiz, Santiago
AU  - Kaluđerović, Goran N.
AU  - Prashar, Sanjiv
AU  - Polo-Ceron, Dorian
AU  - Fajardo, Mariano
AU  - Žižak, Željko
AU  - Sabo, Tibor
AU  - Juranić, Zorica
PY  - 2008
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4130
AB  - A variety of substituted titanocene and ansa-titanocene complexes have been synthesized and characterized using traditional methods.
The cytotoxic activity of the different titanocene complexes was tested against tumour cell lines human adenocarcinoma HeLa, human
myelogenous leukemia K562, human malignant melanoma Fem-x and normal immunocompetent cells, peripheral blood mononuclear
cells PBMC. Alkenyl substitution, either on the cyclopentadienyl ring or on the silicon-atom ansa-bridge of the titanocene compounds
[Ti{Me2Si(g5-C5Me4)(g5-C5H3{CMe2CH2CH2CH@CH2})}Cl2] (8), [Ti{Me(CH2@CH)Si(g5-C5Me4)(g5-C5H4)}Cl2] (9) and [Ti(g5-
C5H4{CMe2CH2CH2CH@CH2})2Cl2] (12) showed higher cytotoxic activities (IC50 values from 24  } 3 to 151  } 10 lM) relative to complexes
bearing an additional alkenyl-substituted silyl substituent on the silicon bridge [Ti{Me{(CH2@CH)Me2SiCH2CH2}Si(g5-
C5Me4)(g5-C5H4)}Cl2] (10) and [Ti{Me{(CH2@CH)3SiCH2CH2}Si(g5-C5Me4)(g5-C5H4)}Cl2] (11) which causes a dramatic decrease
of the cytotoxicity (IC50 values from 155  } 9 to >200 lM). In addition, the synthesis of the analogous niobocene complex [Nb(g5-
C5H4{CMe2CH2CH2CH=CH2})2Cl2] (13), is described. Structural studies based on DFT calculations of the most active complexes 8,
9 and 12 and the X-ray crystal structure of 13 are reported.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Cytotoxic studies of substituted titanocene and ansa-titanocene anticancer drugs
VL  - 102
IS  - 8
SP  - 1558
EP  - 1570
DO  - 10.1016/j.jinorgbio.2008.02.001
ER  - 

@article{
author = "Gomez-Ruiz, Santiago and Kaluđerović, Goran N. and Prashar, Sanjiv and Polo-Ceron, Dorian and Fajardo, Mariano and Žižak, Željko and Sabo, Tibor and Juranić, Zorica",
year = "2008",
abstract = "A variety of substituted titanocene and ansa-titanocene complexes have been synthesized and characterized using traditional methods.
The cytotoxic activity of the different titanocene complexes was tested against tumour cell lines human adenocarcinoma HeLa, human
myelogenous leukemia K562, human malignant melanoma Fem-x and normal immunocompetent cells, peripheral blood mononuclear
cells PBMC. Alkenyl substitution, either on the cyclopentadienyl ring or on the silicon-atom ansa-bridge of the titanocene compounds
[Ti{Me2Si(g5-C5Me4)(g5-C5H3{CMe2CH2CH2CH@CH2})}Cl2] (8), [Ti{Me(CH2@CH)Si(g5-C5Me4)(g5-C5H4)}Cl2] (9) and [Ti(g5-
C5H4{CMe2CH2CH2CH@CH2})2Cl2] (12) showed higher cytotoxic activities (IC50 values from 24  } 3 to 151  } 10 lM) relative to complexes
bearing an additional alkenyl-substituted silyl substituent on the silicon bridge [Ti{Me{(CH2@CH)Me2SiCH2CH2}Si(g5-
C5Me4)(g5-C5H4)}Cl2] (10) and [Ti{Me{(CH2@CH)3SiCH2CH2}Si(g5-C5Me4)(g5-C5H4)}Cl2] (11) which causes a dramatic decrease
of the cytotoxicity (IC50 values from 155  } 9 to >200 lM). In addition, the synthesis of the analogous niobocene complex [Nb(g5-
C5H4{CMe2CH2CH2CH=CH2})2Cl2] (13), is described. Structural studies based on DFT calculations of the most active complexes 8,
9 and 12 and the X-ray crystal structure of 13 are reported.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Cytotoxic studies of substituted titanocene and ansa-titanocene anticancer drugs",
volume = "102",
number = "8",
pages = "1558-1570",
doi = "10.1016/j.jinorgbio.2008.02.001"
}

Gomez-Ruiz, S., Kaluđerović, G. N., Prashar, S., Polo-Ceron, D., Fajardo, M., Žižak, Ž., Sabo, T.,& Juranić, Z.. (2008). Cytotoxic studies of substituted titanocene and ansa-titanocene anticancer drugs. in Journal of Inorganic Biochemistry
Elsevier., 102(8), 1558-1570.
https://doi.org/10.1016/j.jinorgbio.2008.02.001

Gomez-Ruiz S, Kaluđerović GN, Prashar S, Polo-Ceron D, Fajardo M, Žižak Ž, Sabo T, Juranić Z. Cytotoxic studies of substituted titanocene and ansa-titanocene anticancer drugs. in Journal of Inorganic Biochemistry. 2008;102(8):1558-1570.
doi:10.1016/j.jinorgbio.2008.02.001 .

Gomez-Ruiz, Santiago, Kaluđerović, Goran N., Prashar, Sanjiv, Polo-Ceron, Dorian, Fajardo, Mariano, Žižak, Željko, Sabo, Tibor, Juranić, Zorica, "Cytotoxic studies of substituted titanocene and ansa-titanocene anticancer drugs" in Journal of Inorganic Biochemistry, 102, no. 8 (2008):1558-1570,
https://doi.org/10.1016/j.jinorgbio.2008.02.001 . .

TY  - JOUR
AU  - Krajčinović, Bojana B.
AU  - Kaluđerović, Goran N.
AU  - Steinborn, Dirk
AU  - Schmidt, Harry
AU  - Wagner, Christoph
AU  - Žižak, Željko
AU  - Juranić, Zorica
AU  - Trifunović, Srećko R.
AU  - Sabo, Tibor
PY  - 2008
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4330
AB  - Syntheses of two novel ligand precursors O,O′-diisopropyl- (1a) and O,O′-diisobutyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate dihydrochloride monohydrate (1b) and the corresponding dichloroplatinum(II) (2a and 2b) and tetrachloroplatinum(IV) complexes (3a and 3b) are described here. The substances were characterized by IR, 1H and 13C spectroscopy and elemental analysis. Crystal structures were determined for 1a and the corresponding platinum(IV) complex, 3a. In vitro antiproliferative activity was determined against tumor cell lines: human adenocarcinoma HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x, rested and stimulated normal immunocompetent cells (human peripheral blood mononuclear PBMC cells) using KBR test (Kenacid Blue Dye binding test). The IC50(μM) values for the most active compound 3a were: 30.48 ± 2.54; 12.26 ± 2.60; 13.68 ± 3.22; 80.18 ± 24.07 and 71.30 ± 21.70, respectively.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Synthesis and in vitro antitumoral activity of novel O,O′-di-2-alkyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate ligands and corresponding platinum(II/IV) complexes
VL  - 102
IS  - 4
SP  - 892
EP  - 900
DO  - 10.1016/j.jinorgbio.2007.12.009
ER  - 

@article{
author = "Krajčinović, Bojana B. and Kaluđerović, Goran N. and Steinborn, Dirk and Schmidt, Harry and Wagner, Christoph and Žižak, Željko and Juranić, Zorica and Trifunović, Srećko R. and Sabo, Tibor",
year = "2008",
abstract = "Syntheses of two novel ligand precursors O,O′-diisopropyl- (1a) and O,O′-diisobutyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate dihydrochloride monohydrate (1b) and the corresponding dichloroplatinum(II) (2a and 2b) and tetrachloroplatinum(IV) complexes (3a and 3b) are described here. The substances were characterized by IR, 1H and 13C spectroscopy and elemental analysis. Crystal structures were determined for 1a and the corresponding platinum(IV) complex, 3a. In vitro antiproliferative activity was determined against tumor cell lines: human adenocarcinoma HeLa, human myelogenous leukemia K562, human malignant melanoma Fem-x, rested and stimulated normal immunocompetent cells (human peripheral blood mononuclear PBMC cells) using KBR test (Kenacid Blue Dye binding test). The IC50(μM) values for the most active compound 3a were: 30.48 ± 2.54; 12.26 ± 2.60; 13.68 ± 3.22; 80.18 ± 24.07 and 71.30 ± 21.70, respectively.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Synthesis and in vitro antitumoral activity of novel O,O′-di-2-alkyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate ligands and corresponding platinum(II/IV) complexes",
volume = "102",
number = "4",
pages = "892-900",
doi = "10.1016/j.jinorgbio.2007.12.009"
}

Krajčinović, B. B., Kaluđerović, G. N., Steinborn, D., Schmidt, H., Wagner, C., Žižak, Ž., Juranić, Z., Trifunović, S. R.,& Sabo, T.. (2008). Synthesis and in vitro antitumoral activity of novel O,O′-di-2-alkyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate ligands and corresponding platinum(II/IV) complexes. in Journal of Inorganic Biochemistry
Elsevier., 102(4), 892-900.
https://doi.org/10.1016/j.jinorgbio.2007.12.009

Krajčinović BB, Kaluđerović GN, Steinborn D, Schmidt H, Wagner C, Žižak Ž, Juranić Z, Trifunović SR, Sabo T. Synthesis and in vitro antitumoral activity of novel O,O′-di-2-alkyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate ligands and corresponding platinum(II/IV) complexes. in Journal of Inorganic Biochemistry. 2008;102(4):892-900.
doi:10.1016/j.jinorgbio.2007.12.009 .

Krajčinović, Bojana B., Kaluđerović, Goran N., Steinborn, Dirk, Schmidt, Harry, Wagner, Christoph, Žižak, Željko, Juranić, Zorica, Trifunović, Srećko R., Sabo, Tibor, "Synthesis and in vitro antitumoral activity of novel O,O′-di-2-alkyl-(S,S)-ethylenediamine-N,N′-di-2-propanoate ligands and corresponding platinum(II/IV) complexes" in Journal of Inorganic Biochemistry, 102, no. 4 (2008):892-900,
https://doi.org/10.1016/j.jinorgbio.2007.12.009 . .

TY  - JOUR
AU  - Krstić, Natalija
AU  - Bjelaković, Mira
AU  - Žižak, Željko
AU  - Pavlović, Mirjana
AU  - Juranić, Zorica
AU  - Pavlović, Vladimir D.
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/363
AB  - The antiproliferative activity of previously synthesized (Z)-cholest-4-en-6-one oxime (1), (E)-cholest-4-en-6-one oxime (2), 7-aza-B-homocholest-4-en-6-one (3) and 6-aza-B-homocholest-4-en-7-one (4) and newly synthesized 6-thioxo-7-aza-B-homocholest-4-ene (5) and 6-aza-7-thioxo-B-homocholest-4-ene (6) was tested for their possible effects against two human tumor cell lines, cervical carcinoma (HeLa) and chronic myelogenous leukemia (K-562). Compounds 1-6, exerted a dose-dependent antiproliferative effect toward cell lines used in experimental design, showing high selectivity in their action for tumor cells in comparison to normal immunocompetent cells (non-stimulated PBMC and PHA-stimulated PBMC). Compounds 2, 3 and 4 exhibited a very high but selective antitumor activity, by inducing apoptosis in sensitive, for that purpose targeted tumor cell line (HeLa cells). Low toxic effect upon both non-stimulated, and PHA stimulated PBMCs from control, healthy volunteers, has been detected for compounds 1, 2, 3 and 4. The possible reasons for profound differences in the effects of this spectrum of steroidal compounds between tumor cell lines and normal stimulated and non-stimulated PBMCs are discussed. The molecular mechanisms for apoptotic events in HeLa cell line are suggested. The guidelines for further research are underlined.
PB  - Elsevier
T2  - Steroids
T1  - Synthesis of some steroidal oximes, lactams, thiolactams and their antitumor activities
VL  - 72
IS  - 5
SP  - 406
EP  - 414
DO  - 10.1016/j.steroids.2007.02.005
ER  - 

@article{
author = "Krstić, Natalija and Bjelaković, Mira and Žižak, Željko and Pavlović, Mirjana and Juranić, Zorica and Pavlović, Vladimir D.",
year = "2007",
abstract = "The antiproliferative activity of previously synthesized (Z)-cholest-4-en-6-one oxime (1), (E)-cholest-4-en-6-one oxime (2), 7-aza-B-homocholest-4-en-6-one (3) and 6-aza-B-homocholest-4-en-7-one (4) and newly synthesized 6-thioxo-7-aza-B-homocholest-4-ene (5) and 6-aza-7-thioxo-B-homocholest-4-ene (6) was tested for their possible effects against two human tumor cell lines, cervical carcinoma (HeLa) and chronic myelogenous leukemia (K-562). Compounds 1-6, exerted a dose-dependent antiproliferative effect toward cell lines used in experimental design, showing high selectivity in their action for tumor cells in comparison to normal immunocompetent cells (non-stimulated PBMC and PHA-stimulated PBMC). Compounds 2, 3 and 4 exhibited a very high but selective antitumor activity, by inducing apoptosis in sensitive, for that purpose targeted tumor cell line (HeLa cells). Low toxic effect upon both non-stimulated, and PHA stimulated PBMCs from control, healthy volunteers, has been detected for compounds 1, 2, 3 and 4. The possible reasons for profound differences in the effects of this spectrum of steroidal compounds between tumor cell lines and normal stimulated and non-stimulated PBMCs are discussed. The molecular mechanisms for apoptotic events in HeLa cell line are suggested. The guidelines for further research are underlined.",
publisher = "Elsevier",
journal = "Steroids",
title = "Synthesis of some steroidal oximes, lactams, thiolactams and their antitumor activities",
volume = "72",
number = "5",
pages = "406-414",
doi = "10.1016/j.steroids.2007.02.005"
}

Krstić, N., Bjelaković, M., Žižak, Ž., Pavlović, M., Juranić, Z.,& Pavlović, V. D.. (2007). Synthesis of some steroidal oximes, lactams, thiolactams and their antitumor activities. in Steroids
Elsevier., 72(5), 406-414.
https://doi.org/10.1016/j.steroids.2007.02.005

Krstić N, Bjelaković M, Žižak Ž, Pavlović M, Juranić Z, Pavlović VD. Synthesis of some steroidal oximes, lactams, thiolactams and their antitumor activities. in Steroids. 2007;72(5):406-414.
doi:10.1016/j.steroids.2007.02.005 .

Krstić, Natalija, Bjelaković, Mira, Žižak, Željko, Pavlović, Mirjana, Juranić, Zorica, Pavlović, Vladimir D., "Synthesis of some steroidal oximes, lactams, thiolactams and their antitumor activities" in Steroids, 72, no. 5 (2007):406-414,
https://doi.org/10.1016/j.steroids.2007.02.005 . .