TY  - JOUR
AU  - Kosić, Višnja
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Banković, Predrag
AU  - Jović-Jovičić, Nataša
AU  - Knežević-Jugović, Zorica
AU  - Milutinović Nikolić, Aleksandra
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7525
AB  - Three glycoside hydrolases (α-amylase, xylanase and pullulanase) were immobilized on low-cost, environmentally friendly, easily modified clay rich in beidellite. Modifications included common procedures: Na-exchange, acid activation, pillaring, pillaring followed by acid activation, and organo-modifications with chitosan. Supports were characterized by chemical analysis, low temperature N2 physisorption, X-ray powder diffraction (XRPD) and Fourier-transform infrared (FT-IR) spectroscopy. The point of zero charge was also determined. Specific activity of different immobilizates of selected glycoside hydrolases was notably influenced by the type of chemical modification of supports. For each enzyme optimal support was chosen and storage stability was tested. α-Amylase immobilized on acid-activated support retained up to 95% of its initial specific activity of 105.6 ± 5.1 U g−1 after a testing period of 120 days. The most suitable support for xylanase was chitosan-modified beidellite with having specific activity of 90.0 ± 1.4 U g−1 which retained >50% its value after 120 days. Specific activity of pullulanase immobilized on pillared sample that was subsequently activated by acid was 44.5 ± 0.7 U g−1. Initial activity was preserved up to 33% for the same testing period. Comparing these results to the storage stability of the free enzymes that completely lost their activity for the longest period of 40 days, it can be concluded that appropriately modified beidellite- based clays could be used as suitable supports for stabilization of glycoside hydrolases. Nevertheless, further characterization of immobilizates (pH, thermal and operational stability) is needed in order to raise the suitability for larger scale processes in food industry.
PB  - Elsevier
T2  - Applied Clay Science
T1  - Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite
VL  - 250
SP  - 107289
DO  - 10.1016/j.clay.2024.107289
ER  - 

@article{
author = "Kosić, Višnja and Božić, Nataša and Dojnov, Biljana and Banković, Predrag and Jović-Jovičić, Nataša and Knežević-Jugović, Zorica and Milutinović Nikolić, Aleksandra",
year = "2024",
abstract = "Three glycoside hydrolases (α-amylase, xylanase and pullulanase) were immobilized on low-cost, environmentally friendly, easily modified clay rich in beidellite. Modifications included common procedures: Na-exchange, acid activation, pillaring, pillaring followed by acid activation, and organo-modifications with chitosan. Supports were characterized by chemical analysis, low temperature N2 physisorption, X-ray powder diffraction (XRPD) and Fourier-transform infrared (FT-IR) spectroscopy. The point of zero charge was also determined. Specific activity of different immobilizates of selected glycoside hydrolases was notably influenced by the type of chemical modification of supports. For each enzyme optimal support was chosen and storage stability was tested. α-Amylase immobilized on acid-activated support retained up to 95% of its initial specific activity of 105.6 ± 5.1 U g−1 after a testing period of 120 days. The most suitable support for xylanase was chitosan-modified beidellite with having specific activity of 90.0 ± 1.4 U g−1 which retained >50% its value after 120 days. Specific activity of pullulanase immobilized on pillared sample that was subsequently activated by acid was 44.5 ± 0.7 U g−1. Initial activity was preserved up to 33% for the same testing period. Comparing these results to the storage stability of the free enzymes that completely lost their activity for the longest period of 40 days, it can be concluded that appropriately modified beidellite- based clays could be used as suitable supports for stabilization of glycoside hydrolases. Nevertheless, further characterization of immobilizates (pH, thermal and operational stability) is needed in order to raise the suitability for larger scale processes in food industry.",
publisher = "Elsevier",
journal = "Applied Clay Science",
title = "Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite",
volume = "250",
pages = "107289",
doi = "10.1016/j.clay.2024.107289"
}

Kosić, V., Božić, N., Dojnov, B., Banković, P., Jović-Jovičić, N., Knežević-Jugović, Z.,& Milutinović Nikolić, A.. (2024). Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. in Applied Clay Science
Elsevier., 250, 107289.
https://doi.org/10.1016/j.clay.2024.107289

Kosić V, Božić N, Dojnov B, Banković P, Jović-Jovičić N, Knežević-Jugović Z, Milutinović Nikolić A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. in Applied Clay Science. 2024;250:107289.
doi:10.1016/j.clay.2024.107289 .

Kosić, Višnja, Božić, Nataša, Dojnov, Biljana, Banković, Predrag, Jović-Jovičić, Nataša, Knežević-Jugović, Zorica, Milutinović Nikolić, Aleksandra, "Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite" in Applied Clay Science, 250 (2024):107289,
https://doi.org/10.1016/j.clay.2024.107289 . .

TY  - CONF
AU  - Kosić, Višnja
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Stevanović, Gordana
AU  - Banković, Predrag
AU  - Milutinović Nikolić, Aleksandra
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6846
AB  - Clays are naturally occurring, environmentally friendly, chemically inert, thermostable, inexpensive resources that are easily modified into materials with tailored properties. As such, they can be used as suitable supports for enzyme immobilization and application in the food industry. Natural polysaccharides starch, xylan, pullulan, and its derivatives obtained by the action of enzymes, have numerous potentials for food industrial applications. In this work the enzyme supports were prepared from bentonite from Coal mine "Bogovina", Serbia by acid activation (AA), pillaring (P), and pillaring followed by acid activation (PAA). The characterization of the obtained materials included chemical and phase composition, surface acidity, and textural properties. After characterization, -amylase from Bacillus paralicheniformis (BliAmy), commercial xylanase from Sigma-Aldrich (Xyl), and pullulanase from B. paralicheniformis (BliPull) were immobilized on bentonite based supports by 24 h adsorption at 25 °C. The obtained biocatalysts BliAmy-AA (106 IU/g), Xyl-P (74 IU/g), and BliPull-PAA (45 IU/g) showed very good storage stability with the activity preserved after 4 weeks of testing. Products of hydrolysis were detected by TLC and indicate a promising application in the food industry.
PB  - Belgrade : Serbian Chemical Society
C3  - Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023, 210-210
T1  - Stable, environmentally friendly and inexpensive biocatalysts for obtaining important ingredients applicable in the food industry
SP  - 210
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_cer_6846
ER  - 

@conference{
author = "Kosić, Višnja and Božić, Nataša and Dojnov, Biljana and Stevanović, Gordana and Banković, Predrag and Milutinović Nikolić, Aleksandra and Knežević-Jugović, Zorica",
year = "2023",
abstract = "Clays are naturally occurring, environmentally friendly, chemically inert, thermostable, inexpensive resources that are easily modified into materials with tailored properties. As such, they can be used as suitable supports for enzyme immobilization and application in the food industry. Natural polysaccharides starch, xylan, pullulan, and its derivatives obtained by the action of enzymes, have numerous potentials for food industrial applications. In this work the enzyme supports were prepared from bentonite from Coal mine "Bogovina", Serbia by acid activation (AA), pillaring (P), and pillaring followed by acid activation (PAA). The characterization of the obtained materials included chemical and phase composition, surface acidity, and textural properties. After characterization, -amylase from Bacillus paralicheniformis (BliAmy), commercial xylanase from Sigma-Aldrich (Xyl), and pullulanase from B. paralicheniformis (BliPull) were immobilized on bentonite based supports by 24 h adsorption at 25 °C. The obtained biocatalysts BliAmy-AA (106 IU/g), Xyl-P (74 IU/g), and BliPull-PAA (45 IU/g) showed very good storage stability with the activity preserved after 4 weeks of testing. Products of hydrolysis were detected by TLC and indicate a promising application in the food industry.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023, 210-210",
title = "Stable, environmentally friendly and inexpensive biocatalysts for obtaining important ingredients applicable in the food industry",
pages = "210-210",
url = "https://hdl.handle.net/21.15107/rcub_cer_6846"
}

Kosić, V., Božić, N., Dojnov, B., Stevanović, G., Banković, P., Milutinović Nikolić, A.,& Knežević-Jugović, Z.. (2023). Stable, environmentally friendly and inexpensive biocatalysts for obtaining important ingredients applicable in the food industry. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023, 210-210
Belgrade : Serbian Chemical Society., 210-210.
https://hdl.handle.net/21.15107/rcub_cer_6846

Kosić V, Božić N, Dojnov B, Stevanović G, Banković P, Milutinović Nikolić A, Knežević-Jugović Z. Stable, environmentally friendly and inexpensive biocatalysts for obtaining important ingredients applicable in the food industry. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023, 210-210. 2023;:210-210.
https://hdl.handle.net/21.15107/rcub_cer_6846 .

Kosić, Višnja, Božić, Nataša, Dojnov, Biljana, Stevanović, Gordana, Banković, Predrag, Milutinović Nikolić, Aleksandra, Knežević-Jugović, Zorica, "Stable, environmentally friendly and inexpensive biocatalysts for obtaining important ingredients applicable in the food industry" in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia, 2023, 210-210 (2023):210-210,
https://hdl.handle.net/21.15107/rcub_cer_6846 .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Mišić, Milan
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5787
AB  - Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.
PB  - Elsevier Ltd
T2  - Fuel
T1  - Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch
VL  - 338
SP  - 127363
DO  - 10.1016/j.fuel.2022.127363
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Mišić, Milan and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.",
publisher = "Elsevier Ltd",
journal = "Fuel",
title = "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch",
volume = "338",
pages = "127363",
doi = "10.1016/j.fuel.2022.127363"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel
Elsevier Ltd., 338, 127363.
https://doi.org/10.1016/j.fuel.2022.127363

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Mišić M, Božić N, Vujčić Z. Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel. 2023;338:127363.
doi:10.1016/j.fuel.2022.127363 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Mišić, Milan, Božić, Nataša, Vujčić, Zoran, "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch" in Fuel, 338 (2023):127363,
https://doi.org/10.1016/j.fuel.2022.127363 . .

TY  - CONF
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6029
AB  - Since each region of the world has different agricultural sectors, specific residues are generating and at the same time a wide range of alternative novel sources of nutrients emerge, such as carbohydrates, proteins and minerals. Usage of biomass residues as start material for prebiotics production is preferable from the standpoint of ecology and as cheap production process. Corn cob can be considered as the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, with biological benefits such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity. The European Commission has approved XOS produced from corn cob as a safe for human consumption. The corn cob mainly consists of lignocellulose. Chemical treatment is used to extract the hemicelullose from corn cob which is then hydrolyzed to XOS by fungal xylanases. Xylanase produced by fungi genera Aspergillus and Trichoderma are considered key enzymes for XOS production. Fermentation enzyme extract of strain Aspergillus tubingensis FAT35, obtained after SSF on corn cob as substrate, was used for hydrolysis of corn cob xylan to XOS. A. tubigensis FAT35 produced high level of xylanase (350 U/mL). Obtained XOS (2-10 units) were characterized by TLC and by antioxidant activity (ORAC, DPPH, FRAP, and ABTS). Significant antioxidant potential was shown by all used antioxidant tests. The obtained XOS are suitable to be a functional food additive and are obtained from agro-waste material by environmentally and economically suitable way.
PB  - European Federation of Biotechnology
C3  - Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
T1  - Corn cob agro-waste as valuable material for XOS production by fungal enzymes
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_cer_6029
ER  - 

@conference{
author = "Ristović, Marina and Stojanović, Sanja and Šokarda Slavić, Marinela and Margetić, Aleksandra and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Since each region of the world has different agricultural sectors, specific residues are generating and at the same time a wide range of alternative novel sources of nutrients emerge, such as carbohydrates, proteins and minerals. Usage of biomass residues as start material for prebiotics production is preferable from the standpoint of ecology and as cheap production process. Corn cob can be considered as the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, with biological benefits such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity. The European Commission has approved XOS produced from corn cob as a safe for human consumption. The corn cob mainly consists of lignocellulose. Chemical treatment is used to extract the hemicelullose from corn cob which is then hydrolyzed to XOS by fungal xylanases. Xylanase produced by fungi genera Aspergillus and Trichoderma are considered key enzymes for XOS production. Fermentation enzyme extract of strain Aspergillus tubingensis FAT35, obtained after SSF on corn cob as substrate, was used for hydrolysis of corn cob xylan to XOS. A. tubigensis FAT35 produced high level of xylanase (350 U/mL). Obtained XOS (2-10 units) were characterized by TLC and by antioxidant activity (ORAC, DPPH, FRAP, and ABTS). Significant antioxidant potential was shown by all used antioxidant tests. The obtained XOS are suitable to be a functional food additive and are obtained from agro-waste material by environmentally and economically suitable way.",
publisher = "European Federation of Biotechnology",
journal = "Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online",
title = "Corn cob agro-waste as valuable material for XOS production by fungal enzymes",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_cer_6029"
}

Ristović, M., Stojanović, S., Šokarda Slavić, M., Margetić, A., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). Corn cob agro-waste as valuable material for XOS production by fungal enzymes. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
European Federation of Biotechnology., 67-67.
https://hdl.handle.net/21.15107/rcub_cer_6029

Ristović M, Stojanović S, Šokarda Slavić M, Margetić A, Božić N, Vujčić Z, Dojnov B. Corn cob agro-waste as valuable material for XOS production by fungal enzymes. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online. 2023;:67-67.
https://hdl.handle.net/21.15107/rcub_cer_6029 .

Ristović, Marina, Stojanović, Sanja, Šokarda Slavić, Marinela, Margetić, Aleksandra, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "Corn cob agro-waste as valuable material for XOS production by fungal enzymes" in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online (2023):67-67,
https://hdl.handle.net/21.15107/rcub_cer_6029 .

TY  - CONF
AU  - Kosić, Višnja
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Stevanović, Gordana
AU  - Milutinović Nikolić, Aleksandra
AU  - Knežević-Jugović, Zorica
AU  - Banković, Predrag
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6030
AB  - Some coal mines suffer from problem of having huge deposits of bentonite that they regard as undesirable, since bentonite can cause problem due to its swelling property. Instead of piling stacks of bentonite and regarding it as ecological problem the bentonite can be used as enzyme support by immobilization of enzymes as the challenging new application in the field of biotechnology. The enzyme catalysts in the industrial application present lower energy costs and are more environmentally friendly in comparison to traditional chemical processes. The advantages of enzymes are even more prominent when enzymes are applied in immobilized form. In this work the enzyme supports were prepared from bentonite from Coal mine "Bogovina", Serbia where bentonite, although valuable resource, is still considered to be tailings. Bentonite was modified by common methods: acid activation (AA), pillaring (P), and pillaring followed by acid activation (PAA) and tested as enzyme support. All the obtained materials were characterized by the X-ray powder diffraction and FTIR spectroscopy. The amylase, xylanase, and pullulanase were immobilized on different bentonite based supports by 24 h adsorption at 25 °C. The experimental results revealed that under the investigated conditions AA, P, and PAA, were the most suitable for amylase (106 IU/g), xylanase (74 IU/g), and pullulanase (45 IU/g) immobilization, respectively.
PB  - European Federation of Biotechnology
C3  - Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
T1  - The tailings from coal mine instead of waste became applicable as enzyme supports
SP  - 65
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_cer_6030
ER  - 

@conference{
author = "Kosić, Višnja and Božić, Nataša and Dojnov, Biljana and Stevanović, Gordana and Milutinović Nikolić, Aleksandra and Knežević-Jugović, Zorica and Banković, Predrag",
year = "2023",
abstract = "Some coal mines suffer from problem of having huge deposits of bentonite that they regard as undesirable, since bentonite can cause problem due to its swelling property. Instead of piling stacks of bentonite and regarding it as ecological problem the bentonite can be used as enzyme support by immobilization of enzymes as the challenging new application in the field of biotechnology. The enzyme catalysts in the industrial application present lower energy costs and are more environmentally friendly in comparison to traditional chemical processes. The advantages of enzymes are even more prominent when enzymes are applied in immobilized form. In this work the enzyme supports were prepared from bentonite from Coal mine "Bogovina", Serbia where bentonite, although valuable resource, is still considered to be tailings. Bentonite was modified by common methods: acid activation (AA), pillaring (P), and pillaring followed by acid activation (PAA) and tested as enzyme support. All the obtained materials were characterized by the X-ray powder diffraction and FTIR spectroscopy. The amylase, xylanase, and pullulanase were immobilized on different bentonite based supports by 24 h adsorption at 25 °C. The experimental results revealed that under the investigated conditions AA, P, and PAA, were the most suitable for amylase (106 IU/g), xylanase (74 IU/g), and pullulanase (45 IU/g) immobilization, respectively.",
publisher = "European Federation of Biotechnology",
journal = "Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online",
title = "The tailings from coal mine instead of waste became applicable as enzyme supports",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_cer_6030"
}

Kosić, V., Božić, N., Dojnov, B., Stevanović, G., Milutinović Nikolić, A., Knežević-Jugović, Z.,& Banković, P.. (2023). The tailings from coal mine instead of waste became applicable as enzyme supports. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
European Federation of Biotechnology., 65-65.
https://hdl.handle.net/21.15107/rcub_cer_6030

Kosić V, Božić N, Dojnov B, Stevanović G, Milutinović Nikolić A, Knežević-Jugović Z, Banković P. The tailings from coal mine instead of waste became applicable as enzyme supports. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online. 2023;:65-65.
https://hdl.handle.net/21.15107/rcub_cer_6030 .

Kosić, Višnja, Božić, Nataša, Dojnov, Biljana, Stevanović, Gordana, Milutinović Nikolić, Aleksandra, Knežević-Jugović, Zorica, Banković, Predrag, "The tailings from coal mine instead of waste became applicable as enzyme supports" in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online (2023):65-65,
https://hdl.handle.net/21.15107/rcub_cer_6030 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6242
AB  - Most of the functional oligosaccharides (OS) consist of monomers, present in varying degrees of polymerization (DP) ranging between 3 and 10 units. DP of inulin-type fructooligosaccharides has a great impact on fermentability and their
utilization by probiotic bacteria such is Bifidobacteria, thus they have a great impact on their health-promoting effect. Technological properties of fructooligosaccharides (FOS) can improve the physicochemical and sensory characteristics of food products, leading to their increased application in the food industry. It has been found that microbial endoinulinase plays an important role in production of inulin-type fructooligosaccharides. Aspergillus welwitschiae FAW1 strain has proven to be non-toxigenic with the absence of biosynthetic gene clusters for mycotoxins (ochratoxins and fumonisins) and therefore safe for use in food production [5]. Growing on the natural substrate, triticale (Triticosecale sp) FAW1 strain produced inulinase complex from which endoinulinase (InuA) was purified by chromatographic techniques. FOS was prepared by time-controlled hydrolysis of inulin. Monitoring kinetics and determining the amount of obtained FOS by TLC and HPLC methods led to a conclusion that FOS production by hydrolysis of inulin is kinetic dependent reaction. Depending on the reaction time, FOS with different compositions are obtained.The largest amount of produced FOS (DP 2-6) has been in 15-20 minutes of the reaction, where the resulting mixture contains small amount of mono- and disaccharides. The obtained FOS were characterized on antioxidant capacity. Produced FOS showed significant antioxidant potential according to ORAC method which classifies them as potent candidate as additives in functional food. Endoinulinase (InuA) form A. welvitscihae FAW1 considered as key enzyme in FOS preparation. The composition and lenght of the produced FOS can be varied by controlling the reaction time, depending on the needs of of the market and their eventual application.
PB  - Serbian Chemical Society
C3  - Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase
SP  - 203
EP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_cer_6242
ER  - 

@conference{
author = "Stojanović, Sanja and Margetić, Aleksandra and Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Most of the functional oligosaccharides (OS) consist of monomers, present in varying degrees of polymerization (DP) ranging between 3 and 10 units. DP of inulin-type fructooligosaccharides has a great impact on fermentability and their
utilization by probiotic bacteria such is Bifidobacteria, thus they have a great impact on their health-promoting effect. Technological properties of fructooligosaccharides (FOS) can improve the physicochemical and sensory characteristics of food products, leading to their increased application in the food industry. It has been found that microbial endoinulinase plays an important role in production of inulin-type fructooligosaccharides. Aspergillus welwitschiae FAW1 strain has proven to be non-toxigenic with the absence of biosynthetic gene clusters for mycotoxins (ochratoxins and fumonisins) and therefore safe for use in food production [5]. Growing on the natural substrate, triticale (Triticosecale sp) FAW1 strain produced inulinase complex from which endoinulinase (InuA) was purified by chromatographic techniques. FOS was prepared by time-controlled hydrolysis of inulin. Monitoring kinetics and determining the amount of obtained FOS by TLC and HPLC methods led to a conclusion that FOS production by hydrolysis of inulin is kinetic dependent reaction. Depending on the reaction time, FOS with different compositions are obtained.The largest amount of produced FOS (DP 2-6) has been in 15-20 minutes of the reaction, where the resulting mixture contains small amount of mono- and disaccharides. The obtained FOS were characterized on antioxidant capacity. Produced FOS showed significant antioxidant potential according to ORAC method which classifies them as potent candidate as additives in functional food. Endoinulinase (InuA) form A. welvitscihae FAW1 considered as key enzyme in FOS preparation. The composition and lenght of the produced FOS can be varied by controlling the reaction time, depending on the needs of of the market and their eventual application.",
publisher = "Serbian Chemical Society",
journal = "Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase",
pages = "203-203",
url = "https://hdl.handle.net/21.15107/rcub_cer_6242"
}

Stojanović, S., Margetić, A., Šokarda Slavić, M., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Serbian Chemical Society., 203-203.
https://hdl.handle.net/21.15107/rcub_cer_6242

Stojanović S, Margetić A, Šokarda Slavić M, Božić N, Vujčić Z, Dojnov B. Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:203-203.
https://hdl.handle.net/21.15107/rcub_cer_6242 .

Stojanović, Sanja, Margetić, Aleksandra, Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase" in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):203-203,
https://hdl.handle.net/21.15107/rcub_cer_6242 .

TY  - CONF
AU  - Ristović, Marina
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Šokarda Slavić, Marinela
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7135
AB  - Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitation from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process.
PB  - Elsevier
C3  - Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany
T1  - The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production
SP  - P2.002
UR  - https://hdl.handle.net/21.15107/rcub_cer_7135
ER  - 

@conference{
author = "Ristović, Marina and Margetić, Aleksandra and Stojanović, Sanja and Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitation from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process.",
publisher = "Elsevier",
journal = "Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany",
title = "The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production",
pages = "P2.002",
url = "https://hdl.handle.net/21.15107/rcub_cer_7135"
}

Ristović, M., Margetić, A., Stojanović, S., Šokarda Slavić, M., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production. in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany
Elsevier., P2.002.
https://hdl.handle.net/21.15107/rcub_cer_7135

Ristović M, Margetić A, Stojanović S, Šokarda Slavić M, Božić N, Vujčić Z, Dojnov B. The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production. in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany. 2023;:P2.002.
https://hdl.handle.net/21.15107/rcub_cer_7135 .

Ristović, Marina, Margetić, Aleksandra, Stojanović, Sanja, Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production" in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany (2023):P2.002,
https://hdl.handle.net/21.15107/rcub_cer_7135 .

TY  - CONF
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Ristović, Marina
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7137
AB  - Given the growing concern about the depletion of fossil fuels, global warming, and the loss of natural resources, bioethanol made from sugar cane, molasses, and corn continues to garner interest globally and is regarded as the safest and cleanest alternative to oil. Starch is a widely available renewable carbohydrate from which bioethanol is conventionally obtained through energy demanding liquefaction and saccharification processes. A significant simplification of the process and a reduction of starch processing costs would be possible by applying raw starch hydrolysis using enzymes capable of degrading starch below the gelatinization temperature. A novel strategy for highly concentrated raw corn starch (30 % w/v) hydrolysis based on a modified simultaneous saccharification and fermentation process is optimized for the production of bioethanol. Different ratios of Bacillus paralicheniformis ATCC 9945a (BliAmy) and glucoamylase (Dextrozyme® GA), glucoamylase addition time, incubation time, and pH were investigated using a Box–Behnken experimental design to ensure high process efficiency. A two-step synergistic hydrolysis and fermentation with Saccharomyces cerevisiae at 30 °C was carried out in a single bioreactor vessel at the same pH (4.5). The obtained bioethanol concentration at 129.2 g/L, with a productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield indicates the viability of the proposed innovative process.
PB  - Slovenian Biochemical Society
C3  - Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia
T1  - An innovative process for the production of bioethanol: Optimization and kinetic assessment
SP  - P59
UR  - https://hdl.handle.net/21.15107/rcub_cer_7137
ER  - 

@conference{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Ristović, Marina and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Given the growing concern about the depletion of fossil fuels, global warming, and the loss of natural resources, bioethanol made from sugar cane, molasses, and corn continues to garner interest globally and is regarded as the safest and cleanest alternative to oil. Starch is a widely available renewable carbohydrate from which bioethanol is conventionally obtained through energy demanding liquefaction and saccharification processes. A significant simplification of the process and a reduction of starch processing costs would be possible by applying raw starch hydrolysis using enzymes capable of degrading starch below the gelatinization temperature. A novel strategy for highly concentrated raw corn starch (30 % w/v) hydrolysis based on a modified simultaneous saccharification and fermentation process is optimized for the production of bioethanol. Different ratios of Bacillus paralicheniformis ATCC 9945a (BliAmy) and glucoamylase (Dextrozyme® GA), glucoamylase addition time, incubation time, and pH were investigated using a Box–Behnken experimental design to ensure high process efficiency. A two-step synergistic hydrolysis and fermentation with Saccharomyces cerevisiae at 30 °C was carried out in a single bioreactor vessel at the same pH (4.5). The obtained bioethanol concentration at 129.2 g/L, with a productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield indicates the viability of the proposed innovative process.",
publisher = "Slovenian Biochemical Society",
journal = "Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia",
title = "An innovative process for the production of bioethanol: Optimization and kinetic assessment",
pages = "P59",
url = "https://hdl.handle.net/21.15107/rcub_cer_7137"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Ristović, M., Božić, N.,& Vujčić, Z.. (2023). An innovative process for the production of bioethanol: Optimization and kinetic assessment. in Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia
Slovenian Biochemical Society., P59.
https://hdl.handle.net/21.15107/rcub_cer_7137

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Ristović M, Božić N, Vujčić Z. An innovative process for the production of bioethanol: Optimization and kinetic assessment. in Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia. 2023;:P59.
https://hdl.handle.net/21.15107/rcub_cer_7137 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Ristović, Marina, Božić, Nataša, Vujčić, Zoran, "An innovative process for the production of bioethanol: Optimization and kinetic assessment" in Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia (2023):P59,
https://hdl.handle.net/21.15107/rcub_cer_7137 .

TY  - JOUR
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Slomo, Katarina
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5593
AB  - Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.
PB  - Informa UK Limited
T2  - Journal of Apicultural Research
T1  - In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed
VL  - 62
IS  - 5
SP  - 1007
EP  - 1016
DO  - 10.1080/00218839.2022.2080950
ER  - 

@article{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Slomo, Katarina and Ristović, Marina and Vujčić, Zoran",
year = "2023",
abstract = "Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.",
publisher = "Informa UK Limited",
journal = "Journal of Apicultural Research",
title = "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed",
volume = "62",
number = "5",
pages = "1007-1016",
doi = "10.1080/00218839.2022.2080950"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Slomo, K., Ristović, M.,& Vujčić, Z.. (2023). In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research
Informa UK Limited., 62(5), 1007-1016.
https://doi.org/10.1080/00218839.2022.2080950

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Slomo K, Ristović M, Vujčić Z. In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research. 2023;62(5):1007-1016.
doi:10.1080/00218839.2022.2080950 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Slomo, Katarina, Ristović, Marina, Vujčić, Zoran, "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed" in Journal of Apicultural Research, 62, no. 5 (2023):1007-1016,
https://doi.org/10.1080/00218839.2022.2080950 . .

TY  - CONF
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Pavlović, Marija
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6243
AB  - Xylan makes a significant part of cereals and fruits, which are used in the food industry. Therefore, enzymes that hydrolyze xylan (xylanases) have found application in the modification of cereal-based food, improving the digestibility of animal feed, and improving the texture of bakery products. In the juice industry, the main problems are turbidity, viscosity, and sedimentation during standing, which are caused by polysaccharides present in fruit (pectins, cellulose, and hemicellulose (xylan)). Pineapple, apple, orange, and tomato have a high content of hemicellulose, so xylanases are suitable for improving the properties of these juices. The Aspergillus tubingensis FAT 35 strain (considered safe for use in the food industry) growing on SSF medium composed of corn cob produced a high level of xylanase enzyme (4.03 U∕mL) and not that high pectinase (1.02 U∕mL) and cellulase (1.43 U∕mL) activities at pH 3 which is pH of freshly prepared apple, pineapple and organge juice.. The fermentation extract was used for clarification of pineapple, apple, and orange juice and for increasing the filtration rate and yield of these juices. Results indicate that A. tubigensis xylanase could be used for clarification and improvement of properties of juices of fruits that contain hemicellulose in high proportion.
PB  - Serbian Chemical Society
C3  - Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Highly active xylanase used in juice clarification
SP  - 208
EP  - 208
UR  - https://hdl.handle.net/21.15107/rcub_cer_6243
ER  - 

@conference{
author = "Ristović, Marina and Stojanović, Sanja and Pavlović, Marija and Margetić, Aleksandra and Šokarda Slavić, Marinela and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Xylan makes a significant part of cereals and fruits, which are used in the food industry. Therefore, enzymes that hydrolyze xylan (xylanases) have found application in the modification of cereal-based food, improving the digestibility of animal feed, and improving the texture of bakery products. In the juice industry, the main problems are turbidity, viscosity, and sedimentation during standing, which are caused by polysaccharides present in fruit (pectins, cellulose, and hemicellulose (xylan)). Pineapple, apple, orange, and tomato have a high content of hemicellulose, so xylanases are suitable for improving the properties of these juices. The Aspergillus tubingensis FAT 35 strain (considered safe for use in the food industry) growing on SSF medium composed of corn cob produced a high level of xylanase enzyme (4.03 U∕mL) and not that high pectinase (1.02 U∕mL) and cellulase (1.43 U∕mL) activities at pH 3 which is pH of freshly prepared apple, pineapple and organge juice.. The fermentation extract was used for clarification of pineapple, apple, and orange juice and for increasing the filtration rate and yield of these juices. Results indicate that A. tubigensis xylanase could be used for clarification and improvement of properties of juices of fruits that contain hemicellulose in high proportion.",
publisher = "Serbian Chemical Society",
journal = "Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Highly active xylanase used in juice clarification",
pages = "208-208",
url = "https://hdl.handle.net/21.15107/rcub_cer_6243"
}

Ristović, M., Stojanović, S., Pavlović, M., Margetić, A., Šokarda Slavić, M., Vujčić, Z.,& Dojnov, B.. (2023). Highly active xylanase used in juice clarification. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Serbian Chemical Society., 208-208.
https://hdl.handle.net/21.15107/rcub_cer_6243

Ristović M, Stojanović S, Pavlović M, Margetić A, Šokarda Slavić M, Vujčić Z, Dojnov B. Highly active xylanase used in juice clarification. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:208-208.
https://hdl.handle.net/21.15107/rcub_cer_6243 .

Ristović, Marina, Stojanović, Sanja, Pavlović, Marija, Margetić, Aleksandra, Šokarda Slavić, Marinela, Vujčić, Zoran, Dojnov, Biljana, "Highly active xylanase used in juice clarification" in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):208-208,
https://hdl.handle.net/21.15107/rcub_cer_6243 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Stepanović, Jelena
AU  - Ristović, Marina
AU  - Dojnov, Biljana
AU  - Božić, Nataša
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5920
AB  - Fungal inulinases have wide application in industrial biotechnology, and it is presumed that their expression is regulated at the transcriptional level via promoter. It is also known that different sugars have an inducing effect on gene expression in fungal genome, including inulinases. Aim of this work was to determine which of the sugars used in growth medium, as the only carbon source, induce the extracellular exoinulinase gene inuE expression in Aspergillus welwitschiae FAW1. Inulin, rafinose, sucrose, glucose and fructose were used as carbon sources, and expression of inuE was monitored during 72 h of cultivation (tested after 24, 36, 48 and 72 h). Both, presence of mRNA in the mycelia and extracellular enzyme activity in the growth media were monitored. Interestingly,
obtained results showed that inuE was induced by fructose, sucrose and rafinose and not by inulin. In all cases, the highest mRNA was detected after 24 h of cultivation, while extracellular exoinulinase activity increased from 24 h with a peak in 72 h. Further experiments are necessary for a comprehensive understanding of the regulation mechanisms of AweinuE promoter for its more purposeful application in biotechnology.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022
T1  - Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_cer_5920
ER  - 

@conference{
author = "Stojanović, Sanja and Stepanović, Jelena and Ristović, Marina and Dojnov, Biljana and Božić, Nataša and Duduk, Bojan and Vujčić, Zoran",
year = "2022",
abstract = "Fungal inulinases have wide application in industrial biotechnology, and it is presumed that their expression is regulated at the transcriptional level via promoter. It is also known that different sugars have an inducing effect on gene expression in fungal genome, including inulinases. Aim of this work was to determine which of the sugars used in growth medium, as the only carbon source, induce the extracellular exoinulinase gene inuE expression in Aspergillus welwitschiae FAW1. Inulin, rafinose, sucrose, glucose and fructose were used as carbon sources, and expression of inuE was monitored during 72 h of cultivation (tested after 24, 36, 48 and 72 h). Both, presence of mRNA in the mycelia and extracellular enzyme activity in the growth media were monitored. Interestingly,
obtained results showed that inuE was induced by fructose, sucrose and rafinose and not by inulin. In all cases, the highest mRNA was detected after 24 h of cultivation, while extracellular exoinulinase activity increased from 24 h with a peak in 72 h. Further experiments are necessary for a comprehensive understanding of the regulation mechanisms of AweinuE promoter for its more purposeful application in biotechnology.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022",
title = "Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_cer_5920"
}

Stojanović, S., Stepanović, J., Ristović, M., Dojnov, B., Božić, N., Duduk, B.,& Vujčić, Z.. (2022). Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022
University of Belgrade - Faculty of Chemistry., 141.
https://hdl.handle.net/21.15107/rcub_cer_5920

Stojanović S, Stepanović J, Ristović M, Dojnov B, Božić N, Duduk B, Vujčić Z. Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022. 2022;:141.
https://hdl.handle.net/21.15107/rcub_cer_5920 .

Stojanović, Sanja, Stepanović, Jelena, Ristović, Marina, Dojnov, Biljana, Božić, Nataša, Duduk, Bojan, Vujčić, Zoran, "Exoinulinase gene expression in Aspergillus welwitschiae FAW1 induced by different carbon sources" in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia, 2022 (2022):141,
https://hdl.handle.net/21.15107/rcub_cer_5920 .

TY  - CONF
AU  - Kosić, Višnja
AU  - Pavlović, Stefan
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Stevanović, Gordana
AU  - Knežević-Jugović, Zorica
AU  - Milutinović Nikolić, Aleksandra
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5925
AB  - α-Amylase from Bacillus paralicheniformis (BliAmy) is a highly efficient raw starch digesting enzyme. Starch is an inexpensive source of many food industrial products. Naturally occurring clay are non-toxic, environmentally friendly and inexpensive. Therefore, immobilization of BliAmy by adsorption on three differently modified bentonites was studied. Modifications included common Na-exchange procedure, acid activation, and alkali activation. The modified clays were characterized by X-ray powder diffraction, mercury intrusion porosimetry and the points of zero charge were determined. The adsorption of the enzyme was significantly influenced by the type of modification of bentonite, being the highest for the acid-activated bentonite with the highest porosity. On the other hand, the highest enzyme activity for immobilized α-amylase was obtained with alkali-modified bentonite (98 U/g), suggesting it as a good candidate for immobilization of α-amylase for application in the food industry.
PB  - The Society of Physical Chemists of Serbia
C3  - Proceedings - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Physical Chemistry 2022, September, 26-30, 2022, Belgrade, Serbia
T1  - Immobilization of α-amylase from bacillus paralicheniformis on bentonites
SP  - 161
EP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_cer_5925
ER  - 

@conference{
author = "Kosić, Višnja and Pavlović, Stefan and Božić, Nataša and Dojnov, Biljana and Stevanović, Gordana and Knežević-Jugović, Zorica and Milutinović Nikolić, Aleksandra",
year = "2022",
abstract = "α-Amylase from Bacillus paralicheniformis (BliAmy) is a highly efficient raw starch digesting enzyme. Starch is an inexpensive source of many food industrial products. Naturally occurring clay are non-toxic, environmentally friendly and inexpensive. Therefore, immobilization of BliAmy by adsorption on three differently modified bentonites was studied. Modifications included common Na-exchange procedure, acid activation, and alkali activation. The modified clays were characterized by X-ray powder diffraction, mercury intrusion porosimetry and the points of zero charge were determined. The adsorption of the enzyme was significantly influenced by the type of modification of bentonite, being the highest for the acid-activated bentonite with the highest porosity. On the other hand, the highest enzyme activity for immobilized α-amylase was obtained with alkali-modified bentonite (98 U/g), suggesting it as a good candidate for immobilization of α-amylase for application in the food industry.",
publisher = "The Society of Physical Chemists of Serbia",
journal = "Proceedings - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Physical Chemistry 2022, September, 26-30, 2022, Belgrade, Serbia",
title = "Immobilization of α-amylase from bacillus paralicheniformis on bentonites",
pages = "161-164",
url = "https://hdl.handle.net/21.15107/rcub_cer_5925"
}

Kosić, V., Pavlović, S., Božić, N., Dojnov, B., Stevanović, G., Knežević-Jugović, Z.,& Milutinović Nikolić, A.. (2022). Immobilization of α-amylase from bacillus paralicheniformis on bentonites. in Proceedings - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Physical Chemistry 2022, September, 26-30, 2022, Belgrade, Serbia
The Society of Physical Chemists of Serbia., 161-164.
https://hdl.handle.net/21.15107/rcub_cer_5925

Kosić V, Pavlović S, Božić N, Dojnov B, Stevanović G, Knežević-Jugović Z, Milutinović Nikolić A. Immobilization of α-amylase from bacillus paralicheniformis on bentonites. in Proceedings - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Physical Chemistry 2022, September, 26-30, 2022, Belgrade, Serbia. 2022;:161-164.
https://hdl.handle.net/21.15107/rcub_cer_5925 .

Kosić, Višnja, Pavlović, Stefan, Božić, Nataša, Dojnov, Biljana, Stevanović, Gordana, Knežević-Jugović, Zorica, Milutinović Nikolić, Aleksandra, "Immobilization of α-amylase from bacillus paralicheniformis on bentonites" in Proceedings - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Physical Chemistry 2022, September, 26-30, 2022, Belgrade, Serbia (2022):161-164,
https://hdl.handle.net/21.15107/rcub_cer_5925 .

TY  - CONF
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Tomić, Nevena
AU  - Mišić, Milan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5885
AB  - Feeding of honey bee (Apis mellifera) is a challenge for beekeepers and formulation of food supplements is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial (sugar) patty and the patties enriched with 12.5% pollen and 12.5% dried yeast on protease expression in honey bee adults is presented. This is part of a wider study aimed to compare the expression of digestive enzymes using different patties. Data collected in this study can be useful for development of higher quality of food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage there were one hundred bees and a piece of honeycomb. Midgut samples were taken after 7, 14 and 21 days, homogenized and analyzed for protein concentration and protease activity.
The highest protein concentration was detected in the midgut of pollen fed bees, and lowest in commercial patty fed group, determined by the Bradford method. Protease activity was the highest in bee’s midgut fed with pollen patties, and the lowest in bees fed commercial patties which is shown by enzyme assay and by zymograms. There were different protease isoforms present in bees fed pollen, yeast and commercial patties, but the major isoforms were the same.
The observed decrease in protease activity over time in all groups is probably due to the transition to foraging tasks. Reduced protease activity in the gut of bees fed commercial patties is due to a lack of proteins or some other inducers present in food.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen (dried yeast) can be considered as appropriate food substitution, because it is superior to supplements which induce very different enzyme expression.
PB  - University of Belgrade – Faculty of Biology
C3  - Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia
T1  - Expression of protease in adult honey bees fed with different patties
SP  - 172
UR  - https://hdl.handle.net/21.15107/rcub_cer_5885
ER  - 

@conference{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Tomić, Nevena and Mišić, Milan and Vujčić, Zoran",
year = "2022",
abstract = "Feeding of honey bee (Apis mellifera) is a challenge for beekeepers and formulation of food supplements is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial (sugar) patty and the patties enriched with 12.5% pollen and 12.5% dried yeast on protease expression in honey bee adults is presented. This is part of a wider study aimed to compare the expression of digestive enzymes using different patties. Data collected in this study can be useful for development of higher quality of food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage there were one hundred bees and a piece of honeycomb. Midgut samples were taken after 7, 14 and 21 days, homogenized and analyzed for protein concentration and protease activity.
The highest protein concentration was detected in the midgut of pollen fed bees, and lowest in commercial patty fed group, determined by the Bradford method. Protease activity was the highest in bee’s midgut fed with pollen patties, and the lowest in bees fed commercial patties which is shown by enzyme assay and by zymograms. There were different protease isoforms present in bees fed pollen, yeast and commercial patties, but the major isoforms were the same.
The observed decrease in protease activity over time in all groups is probably due to the transition to foraging tasks. Reduced protease activity in the gut of bees fed commercial patties is due to a lack of proteins or some other inducers present in food.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen (dried yeast) can be considered as appropriate food substitution, because it is superior to supplements which induce very different enzyme expression.",
publisher = "University of Belgrade – Faculty of Biology",
journal = "Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia",
title = "Expression of protease in adult honey bees fed with different patties",
pages = "172",
url = "https://hdl.handle.net/21.15107/rcub_cer_5885"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Tomić, N., Mišić, M.,& Vujčić, Z.. (2022). Expression of protease in adult honey bees fed with different patties. in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia
University of Belgrade – Faculty of Biology., 172.
https://hdl.handle.net/21.15107/rcub_cer_5885

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Tomić N, Mišić M, Vujčić Z. Expression of protease in adult honey bees fed with different patties. in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia. 2022;:172.
https://hdl.handle.net/21.15107/rcub_cer_5885 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Tomić, Nevena, Mišić, Milan, Vujčić, Zoran, "Expression of protease in adult honey bees fed with different patties" in Abstract Book - 9th European Congress of Apidology - EurBee 9, September 20-22, 2022, Belgrade, Serbia (2022):172,
https://hdl.handle.net/21.15107/rcub_cer_5885 .

TY  - JOUR
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Stepanović, Jelena
AU  - Margetić, Aleksandra
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5360
AB  - Production of fructooligosaccharides (FOS) is a trending topic due to their prebiotic effect becoming increasingly important for the modern human diet. The most suitable process for FOS production is the one using fungal inulinases. Introduction of new fungal inulinase producers and their implementation in production of inulinase enzymes is therefore gaining interest. This study provides a new approach to FOS synthesis by fungal enzyme complex without prior separation of any specific enzyme. Inulinase enzyme complexes could be used for the synthesis of FOS in two possible ways – hydrolysis of inulin (FOSh) and transfructosylation process of sucrose (FOSs), as demonstrated here. Depending on the fungal growth inducing substrate, a variety of inulinase enzyme complexes was obtained – one of which was most successful in production of FOSh and another one of FOSs. Substrates derived from crops: triticale, wheat bran, Jerusalem artichoke and Aspergillus welwitschiae isolate, previously proven as safe for use in food, were utilized for production of inulinase enzyme cocktails. The highest FOSs production was obtained by enzyme complex rich in β-fructofuranosidase, while the highest FOSh production was obtained by enzyme complex rich in endoinulinase. Both FOSh and FOSs showed antioxidant potential according to ABTS and ORAC, which classifies them as a suitable additive in functional food. Simultaneous zymographic detection of inulinase enzymes, which could contribute to expansion of the knowledge on fungal enzymes, was developed and applied here. It demonstrated the presence of different inulinase isoforms depending on fungal growth substrate. These findings, which rely on the innate ability of fungi to co-produce all inulinases from a cocktail, could be useful as a new, easy approach to FOS production by fungal enzymes without their separation and purification, contributing to cheaper and faster production processes.
PB  - Elsevier
T2  - Food Research International
T1  - Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production
VL  - 160
SP  - 111755
DO  - 10.1016/j.foodres.2022.111755
ER  - 

@article{
author = "Stojanović, Sanja and Ristović, Marina and Stepanović, Jelena and Margetić, Aleksandra and Duduk, Bojan and Vujčić, Zoran and Dojnov, Biljana",
year = "2022",
abstract = "Production of fructooligosaccharides (FOS) is a trending topic due to their prebiotic effect becoming increasingly important for the modern human diet. The most suitable process for FOS production is the one using fungal inulinases. Introduction of new fungal inulinase producers and their implementation in production of inulinase enzymes is therefore gaining interest. This study provides a new approach to FOS synthesis by fungal enzyme complex without prior separation of any specific enzyme. Inulinase enzyme complexes could be used for the synthesis of FOS in two possible ways – hydrolysis of inulin (FOSh) and transfructosylation process of sucrose (FOSs), as demonstrated here. Depending on the fungal growth inducing substrate, a variety of inulinase enzyme complexes was obtained – one of which was most successful in production of FOSh and another one of FOSs. Substrates derived from crops: triticale, wheat bran, Jerusalem artichoke and Aspergillus welwitschiae isolate, previously proven as safe for use in food, were utilized for production of inulinase enzyme cocktails. The highest FOSs production was obtained by enzyme complex rich in β-fructofuranosidase, while the highest FOSh production was obtained by enzyme complex rich in endoinulinase. Both FOSh and FOSs showed antioxidant potential according to ABTS and ORAC, which classifies them as a suitable additive in functional food. Simultaneous zymographic detection of inulinase enzymes, which could contribute to expansion of the knowledge on fungal enzymes, was developed and applied here. It demonstrated the presence of different inulinase isoforms depending on fungal growth substrate. These findings, which rely on the innate ability of fungi to co-produce all inulinases from a cocktail, could be useful as a new, easy approach to FOS production by fungal enzymes without their separation and purification, contributing to cheaper and faster production processes.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production",
volume = "160",
pages = "111755",
doi = "10.1016/j.foodres.2022.111755"
}

Stojanović, S., Ristović, M., Stepanović, J., Margetić, A., Duduk, B., Vujčić, Z.,& Dojnov, B.. (2022). Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production. in Food Research International
Elsevier., 160, 111755.
https://doi.org/10.1016/j.foodres.2022.111755

Stojanović S, Ristović M, Stepanović J, Margetić A, Duduk B, Vujčić Z, Dojnov B. Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production. in Food Research International. 2022;160:111755.
doi:10.1016/j.foodres.2022.111755 .

Stojanović, Sanja, Ristović, Marina, Stepanović, Jelena, Margetić, Aleksandra, Duduk, Bojan, Vujčić, Zoran, Dojnov, Biljana, "Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production" in Food Research International, 160 (2022):111755,
https://doi.org/10.1016/j.foodres.2022.111755 . .

TY  - JOUR
AU  - Stojanović, Sanja
AU  - Stepanović, Jelena
AU  - Špirović Trifunović, Bojana
AU  - Duduk, Nataša
AU  - Dojnov, Biljana
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5585
AB  - Research background. Inulinases are used for fructooligosaccharide production and they are of interest for both scientific community and industry. Black aspergilli represent a diverse group of species that has use for enzyme production, in particular some species are known as potent inulinase producers. Finding new potential producers from the environment is as important as improving the production with known strains. Safe use of enzymes produced by aspergilli in food industry is placed ahead of their benefit for inulinase production.  Experimental approach. Here we show a specific approach to finding/screening of newly isolated fungal inulinase producers that combines a newly developed screening method and an equally important assessment of the toxigenic potential of the fungus. In this study 39 black aspergilli collected from different substrates in Serbia were identified and assessed for inulinase production.  Results and conclusions. The most common species were Aspergillus tubingensis (51.2 %), followed by A. niger (23.1 %), A. welwitschiae (23.1 %) and A. uvarum (2.6 %). The isolates for inulinase production were selected using a cheap and easy, fast and non-hazardous alternative inulinase screening test developed in this work. Enzymatic activity of selected inulinase-producing strains was confirmed spectrophotometrically. Since some A. niger and A. welwitschiae strains are able to produce mycotoxins ochratoxin A (OTA) and fumonisins (FB), the toxigenic potential of selected inulinase producers was assessed analytically and genetically. Fungal enzyme producer can be considered safe for use in food industry only after comparing the results of both approaches for investigating toxic potential, the direct presence of mycotoxins in the enzyme preparation (analytically) and the presence of mycotoxin gene clusters (genetically). In some strains the absence of OTA and FB production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters, respectively. The two best inulinase producers and mycotoxin non-producers (without mycotoxin production capability as additional safety) were selected as potential candidates for further development of enzyme production.  Novelty and scientific contribution. The presented innovative approach for the selection of potential fungal enzyme producer shows that only non-toxigenic fungi could be considered as useful in food industry. Although this study was done on local isolates, the approach is applicable globally.
PB  - Faculty of Food Technology and Biotechnology - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing
VL  - 60
IS  - 4
SP  - 421
EP  - 433
DO  - 10.17113/ftb.60.04.22.7521
ER  - 

@article{
author = "Stojanović, Sanja and Stepanović, Jelena and Špirović Trifunović, Bojana and Duduk, Nataša and Dojnov, Biljana and Duduk, Bojan and Vujčić, Zoran",
year = "2022",
abstract = "Research background. Inulinases are used for fructooligosaccharide production and they are of interest for both scientific community and industry. Black aspergilli represent a diverse group of species that has use for enzyme production, in particular some species are known as potent inulinase producers. Finding new potential producers from the environment is as important as improving the production with known strains. Safe use of enzymes produced by aspergilli in food industry is placed ahead of their benefit for inulinase production.  Experimental approach. Here we show a specific approach to finding/screening of newly isolated fungal inulinase producers that combines a newly developed screening method and an equally important assessment of the toxigenic potential of the fungus. In this study 39 black aspergilli collected from different substrates in Serbia were identified and assessed for inulinase production.  Results and conclusions. The most common species were Aspergillus tubingensis (51.2 %), followed by A. niger (23.1 %), A. welwitschiae (23.1 %) and A. uvarum (2.6 %). The isolates for inulinase production were selected using a cheap and easy, fast and non-hazardous alternative inulinase screening test developed in this work. Enzymatic activity of selected inulinase-producing strains was confirmed spectrophotometrically. Since some A. niger and A. welwitschiae strains are able to produce mycotoxins ochratoxin A (OTA) and fumonisins (FB), the toxigenic potential of selected inulinase producers was assessed analytically and genetically. Fungal enzyme producer can be considered safe for use in food industry only after comparing the results of both approaches for investigating toxic potential, the direct presence of mycotoxins in the enzyme preparation (analytically) and the presence of mycotoxin gene clusters (genetically). In some strains the absence of OTA and FB production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters, respectively. The two best inulinase producers and mycotoxin non-producers (without mycotoxin production capability as additional safety) were selected as potential candidates for further development of enzyme production.  Novelty and scientific contribution. The presented innovative approach for the selection of potential fungal enzyme producer shows that only non-toxigenic fungi could be considered as useful in food industry. Although this study was done on local isolates, the approach is applicable globally.",
publisher = "Faculty of Food Technology and Biotechnology - University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing",
volume = "60",
number = "4",
pages = "421-433",
doi = "10.17113/ftb.60.04.22.7521"
}

Stojanović, S., Stepanović, J., Špirović Trifunović, B., Duduk, N., Dojnov, B., Duduk, B.,& Vujčić, Z.. (2022). Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing. in Food Technology and Biotechnology
Faculty of Food Technology and Biotechnology - University of Zagreb., 60(4), 421-433.
https://doi.org/10.17113/ftb.60.04.22.7521

Stojanović S, Stepanović J, Špirović Trifunović B, Duduk N, Dojnov B, Duduk B, Vujčić Z. Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing. in Food Technology and Biotechnology. 2022;60(4):421-433.
doi:10.17113/ftb.60.04.22.7521 .

Stojanović, Sanja, Stepanović, Jelena, Špirović Trifunović, Bojana, Duduk, Nataša, Dojnov, Biljana, Duduk, Bojan, Vujčić, Zoran, "Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing" in Food Technology and Biotechnology, 60, no. 4 (2022):421-433,
https://doi.org/10.17113/ftb.60.04.22.7521 . .

TY  - CONF
AU  - Dojnov, Biljana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5861
AB  - Agro waste materials are agricultural residues (steam, stalks, husks...) and food industrial residues (potato, orange, apple and etc peels and soyabean, coconut etc cakes). Since each region of the world has different agricultural sectors, specific residues are generating and at the same time a wide range of alternative novel sorrces of nutrients emerge, such as carbohydrates, proteins and minerals. Proper waste biomass management is one of priority in EU and in world. Filamentous fungi are most potent microorganisms for bioprocessing of
waste materials in purpose to obtain value added products such as enzymes, biofuel, bioactive compounds, prebiotics, chemicals, antibiotics... Genus Aspergillus and Trichoderma are well studied and recognized as potent enzyme producers. Enzyme produced by filamentous fungi such as cellulase, amylase, xylanase, glucosidase, are widely used in industry. Environmentfriendly
and cost effect solution for their obtaining is utilization of agro waste material as
solid substrate for fungal growth. Prebiotics are oligosaccharides with 2-6 units defined as "a substrate that is selectively utilized by host microorganisms conferring a health benefit". Epidemiologic studies have significantly recognized prebiotics as an essential constituent of a healthy diet. Prebiotics, e.g. FOS are prepared by effective fungal enzymes. Usage of biomass residues as start material for prebiotics production is preferable from the standpoint of ecology and as cheap production process. There are number of agro waste materials that
could be used for this propose. Corn cob can be considered as the main source for XOS production.
Fungi are saprophytic eukaryotic organisms, habitat lignocellulosic material in nature and they are capable to induce enzymes depending on growth substrate, which actually enables their usage for in situ prebiotics production, which is new and trending research in this field. This approach unites eu4tme and prebiotics obtaining from agro waste in only one process.
PB  - University of Belgrade - Faculty of Agriculture
C3  - Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food, September 7-9, 2022, Belgrade
T1  - Recycling of agro waste by fungi for obtaining enzymes and prebiotics
SP  - 23
EP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_cer_5861
ER  - 

@conference{
author = "Dojnov, Biljana",
year = "2022",
abstract = "Agro waste materials are agricultural residues (steam, stalks, husks...) and food industrial residues (potato, orange, apple and etc peels and soyabean, coconut etc cakes). Since each region of the world has different agricultural sectors, specific residues are generating and at the same time a wide range of alternative novel sorrces of nutrients emerge, such as carbohydrates, proteins and minerals. Proper waste biomass management is one of priority in EU and in world. Filamentous fungi are most potent microorganisms for bioprocessing of
waste materials in purpose to obtain value added products such as enzymes, biofuel, bioactive compounds, prebiotics, chemicals, antibiotics... Genus Aspergillus and Trichoderma are well studied and recognized as potent enzyme producers. Enzyme produced by filamentous fungi such as cellulase, amylase, xylanase, glucosidase, are widely used in industry. Environmentfriendly
and cost effect solution for their obtaining is utilization of agro waste material as
solid substrate for fungal growth. Prebiotics are oligosaccharides with 2-6 units defined as "a substrate that is selectively utilized by host microorganisms conferring a health benefit". Epidemiologic studies have significantly recognized prebiotics as an essential constituent of a healthy diet. Prebiotics, e.g. FOS are prepared by effective fungal enzymes. Usage of biomass residues as start material for prebiotics production is preferable from the standpoint of ecology and as cheap production process. There are number of agro waste materials that
could be used for this propose. Corn cob can be considered as the main source for XOS production.
Fungi are saprophytic eukaryotic organisms, habitat lignocellulosic material in nature and they are capable to induce enzymes depending on growth substrate, which actually enables their usage for in situ prebiotics production, which is new and trending research in this field. This approach unites eu4tme and prebiotics obtaining from agro waste in only one process.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food, September 7-9, 2022, Belgrade",
title = "Recycling of agro waste by fungi for obtaining enzymes and prebiotics",
pages = "23-23",
url = "https://hdl.handle.net/21.15107/rcub_cer_5861"
}

Dojnov, B.. (2022). Recycling of agro waste by fungi for obtaining enzymes and prebiotics. in Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food, September 7-9, 2022, Belgrade
University of Belgrade - Faculty of Agriculture., 23-23.
https://hdl.handle.net/21.15107/rcub_cer_5861

Dojnov B. Recycling of agro waste by fungi for obtaining enzymes and prebiotics. in Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food, September 7-9, 2022, Belgrade. 2022;:23-23.
https://hdl.handle.net/21.15107/rcub_cer_5861 .

Dojnov, Biljana, "Recycling of agro waste by fungi for obtaining enzymes and prebiotics" in Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food, September 7-9, 2022, Belgrade (2022):23-23,
https://hdl.handle.net/21.15107/rcub_cer_5861 .

TY  - CONF
AU  - Dojnov, Biljana
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5888
AB  - Енергетска транзиција подразумева примену правилног управљања отпадним биомасама које обухвата њено смањење, поновну употребу, рециклирање, добијање енергије и/или одлагање. Наука нуди решења за ефикасну примену поновне употребе и рециклирања, где се добијају производи додатне вредности. У Србији, према проценама, у врстама отпадних биомаса доминирају жетвени остаци (10.140.268 t годишње) и остаци резидбе воћа (74.329 t годишње) а следе стајњак, отпадни материјал из прерадне индустрије и биоразградиви комунални отпад. Биопроцесовање (употреба микроба и ензима) представља најприхватљивије решење заштите животне средине, где се из отпадне биомасе добијају вредни производи (пребиотици, биоактивни пептиди, антибиотици, терпеноиди, алкалоиди, итд.) широке примене у храни, лековима и козметици.
Из отпадног клипа кукуруза (кога у Србији има 1.073.780 t годишње) добијају се пребиотици, одобрени за употребу у исхрани. Искоришћени компост за гајење шампињона је погодан супстрат за микробиолошко добијање индустријски значајних ензима (амилазе, целулазе, ксиланазе). Постоје примери индустрије где се из дрвних остатака (којих има у Србији 700.000 m3 годишње) поред етанола добијају и ацеталдехид, сирћетна киселина и етилацетат. Екстракцијом из остатака индустрије прераде воћа, поврћа и винове лозе се могу добити полифеноли (антиоксиданси) и други вредни производи. Максимална искористљивост отпадне биомасе може се постићи комбиновањем метода биопроцесовања за добијање производа додатне вредности са добијањем енергије (биогаса, биоетанола).
PB  - Српска академија наука и уметности - академијски одбор за енергетику
C3  - Свеска сажетака - Научни скуп - потенцијал и ефекти коришћења биомасе у Републици Србији, 2. и 3. Новембар 2022, Београд
T1  - Добијање производа додатне вредности од отпадне биомасе
SP  - I-7
UR  - https://hdl.handle.net/21.15107/rcub_cer_5888
ER  - 

@conference{
author = "Dojnov, Biljana and Stojanović, Sanja and Ristović, Marina and Vujčić, Zoran",
year = "2022",
abstract = "Енергетска транзиција подразумева примену правилног управљања отпадним биомасама које обухвата њено смањење, поновну употребу, рециклирање, добијање енергије и/или одлагање. Наука нуди решења за ефикасну примену поновне употребе и рециклирања, где се добијају производи додатне вредности. У Србији, према проценама, у врстама отпадних биомаса доминирају жетвени остаци (10.140.268 t годишње) и остаци резидбе воћа (74.329 t годишње) а следе стајњак, отпадни материјал из прерадне индустрије и биоразградиви комунални отпад. Биопроцесовање (употреба микроба и ензима) представља најприхватљивије решење заштите животне средине, где се из отпадне биомасе добијају вредни производи (пребиотици, биоактивни пептиди, антибиотици, терпеноиди, алкалоиди, итд.) широке примене у храни, лековима и козметици.
Из отпадног клипа кукуруза (кога у Србији има 1.073.780 t годишње) добијају се пребиотици, одобрени за употребу у исхрани. Искоришћени компост за гајење шампињона је погодан супстрат за микробиолошко добијање индустријски значајних ензима (амилазе, целулазе, ксиланазе). Постоје примери индустрије где се из дрвних остатака (којих има у Србији 700.000 m3 годишње) поред етанола добијају и ацеталдехид, сирћетна киселина и етилацетат. Екстракцијом из остатака индустрије прераде воћа, поврћа и винове лозе се могу добити полифеноли (антиоксиданси) и други вредни производи. Максимална искористљивост отпадне биомасе може се постићи комбиновањем метода биопроцесовања за добијање производа додатне вредности са добијањем енергије (биогаса, биоетанола).",
publisher = "Српска академија наука и уметности - академијски одбор за енергетику",
journal = "Свеска сажетака - Научни скуп - потенцијал и ефекти коришћења биомасе у Републици Србији, 2. и 3. Новембар 2022, Београд",
title = "Добијање производа додатне вредности од отпадне биомасе",
pages = "I-7",
url = "https://hdl.handle.net/21.15107/rcub_cer_5888"
}

Dojnov, B., Stojanović, S., Ristović, M.,& Vujčić, Z.. (2022). Добијање производа додатне вредности од отпадне биомасе. in Свеска сажетака - Научни скуп - потенцијал и ефекти коришћења биомасе у Републици Србији, 2. и 3. Новембар 2022, Београд
Српска академија наука и уметности - академијски одбор за енергетику., I-7.
https://hdl.handle.net/21.15107/rcub_cer_5888

Dojnov B, Stojanović S, Ristović M, Vujčić Z. Добијање производа додатне вредности од отпадне биомасе. in Свеска сажетака - Научни скуп - потенцијал и ефекти коришћења биомасе у Републици Србији, 2. и 3. Новембар 2022, Београд. 2022;:I-7.
https://hdl.handle.net/21.15107/rcub_cer_5888 .

Dojnov, Biljana, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, "Добијање производа додатне вредности од отпадне биомасе" in Свеска сажетака - Научни скуп - потенцијал и ефекти коришћења биомасе у Републици Србији, 2. и 3. Новембар 2022, Београд (2022):I-7,
https://hdl.handle.net/21.15107/rcub_cer_5888 .

TY  - CONF
AU  - Pavlović, Marija
AU  - Stojanović, Sanja
AU  - Dojnov, Biljana
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5834
AB  - Pectinolytic enzymes represent a large group of enzymes that catalyze the reactions of depolymerization and deesterification of pectin polysaccharides1. Saprophytic fungi produce pectinases on a large scale for industrial purposes. These enzymes have a various biotechnological application and their global annual production represents 25% of total industrial enzymes 1,2. Agro-waste is widely used as economical substrate for the production of pectinases by solid state fermentation 3. In this study, sugar beet pulp, as a good source of pectin3, was used as a substrate for enzyme production by Aspergillus tubingensis. This strain was isolated from the quince fruit and identified by the molecular DNA marker calmodulin (CaM). SSF was performed with this strain on sugar beet pulp (80%) in combination with wheat bran (20%), a potent substrate for pectinase production 3. The obtained high pectinolytic activity (15 U/mL), determined by 3,5-dinitrosalicylic acid reagent, was in the range of commercial pectinases. Zymography detection, using Ruthenium Red to visualize endo-pectinase activity and pectin-methyl esterase activity revealed several pectinase activity bands. Hydrolysis of different pectin substrates with the obtained pectinase complex was analyzed by thin layer chromatography in order to detect different products such as pectic oligosaccharides, which are emerging prebiotics superior to intact pectin.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Highly active pectinases from newly isolated Aspergillus tubingensis strain
SP  - 124
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_cer_5834
ER  - 

@conference{
author = "Pavlović, Marija and Stojanović, Sanja and Dojnov, Biljana and Božić, Nataša and Vujčić, Zoran and Margetić, Aleksandra",
year = "2021",
abstract = "Pectinolytic enzymes represent a large group of enzymes that catalyze the reactions of depolymerization and deesterification of pectin polysaccharides1. Saprophytic fungi produce pectinases on a large scale for industrial purposes. These enzymes have a various biotechnological application and their global annual production represents 25% of total industrial enzymes 1,2. Agro-waste is widely used as economical substrate for the production of pectinases by solid state fermentation 3. In this study, sugar beet pulp, as a good source of pectin3, was used as a substrate for enzyme production by Aspergillus tubingensis. This strain was isolated from the quince fruit and identified by the molecular DNA marker calmodulin (CaM). SSF was performed with this strain on sugar beet pulp (80%) in combination with wheat bran (20%), a potent substrate for pectinase production 3. The obtained high pectinolytic activity (15 U/mL), determined by 3,5-dinitrosalicylic acid reagent, was in the range of commercial pectinases. Zymography detection, using Ruthenium Red to visualize endo-pectinase activity and pectin-methyl esterase activity revealed several pectinase activity bands. Hydrolysis of different pectin substrates with the obtained pectinase complex was analyzed by thin layer chromatography in order to detect different products such as pectic oligosaccharides, which are emerging prebiotics superior to intact pectin.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Highly active pectinases from newly isolated Aspergillus tubingensis strain",
pages = "124-125",
url = "https://hdl.handle.net/21.15107/rcub_cer_5834"
}

Pavlović, M., Stojanović, S., Dojnov, B., Božić, N., Vujčić, Z.,& Margetić, A.. (2021). Highly active pectinases from newly isolated Aspergillus tubingensis strain. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 124-125.
https://hdl.handle.net/21.15107/rcub_cer_5834

Pavlović M, Stojanović S, Dojnov B, Božić N, Vujčić Z, Margetić A. Highly active pectinases from newly isolated Aspergillus tubingensis strain. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:124-125.
https://hdl.handle.net/21.15107/rcub_cer_5834 .

Pavlović, Marija, Stojanović, Sanja, Dojnov, Biljana, Božić, Nataša, Vujčić, Zoran, Margetić, Aleksandra, "Highly active pectinases from newly isolated Aspergillus tubingensis strain" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):124-125,
https://hdl.handle.net/21.15107/rcub_cer_5834 .

TY  - CONF
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Slomo, Katarina
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5886
AB  - Fed of honey bee (Apis mellifera) is challenging for beekeepers and formulation of supplemental food is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial patty and patty enriched with 12.5% pollen on amylase expression in honey bee adults is presented. This
is part of a wider study aimed to compare the activity of digestive enzymes when using different patties. We assume that data collected in this way can be used for the development of better food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage, there were one hundred bees and a piece of honeycomb. Midgut and hindgut samples were taken after 7, 14 and 21 days and midgut without hindgut was taken after 21 days. Samples were homogenized and used for amylase zymogram, IEF and enzyme assays.
There was no mortality during the experiment. A high protein concentration was detected in the midgut in both groups of bees. Amylase activity was significantly higher in bees fed pollen enriched patties, which is shown by enzyme assay and by zymograms. There are different amylase isoforms present in bees fed by pollen enriched patties in comparison to bees fed by commercial patties, but
the major isoforms were the same.
The observed decrease in the amylase activity over time is probably due to dilution caused by the accumulation of water and undigested substances in the hindgut. Reduced amylase activity in the intestines of bees fed by commercial patties is due to a lack of starch or some other inducers present in pollen.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen can be superior in comparison to supplements that induce very different enzyme expression.
PB  - Belgrade : University of Belgrade
C3  - Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia
T1  - Expression of amylases in adult honey bees fed with different patties
SP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_cer_5886
ER  - 

@conference{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Slomo, Katarina and Ristović, Marina and Vujčić, Zoran",
year = "2021",
abstract = "Fed of honey bee (Apis mellifera) is challenging for beekeepers and formulation of supplemental food is improved continuously. When natural food sources are scarce or not available, supplemental foods are widely used to support and build up honey bee colonies. Influence of commercial patty and patty enriched with 12.5% pollen on amylase expression in honey bee adults is presented. This
is part of a wider study aimed to compare the activity of digestive enzymes when using different patties. We assume that data collected in this way can be used for the development of better food supplements for honey bees.
Honey bees were kept in an incubator for 21 days, at a temperature of 35°C and at 80% humidity. In each cage, there were one hundred bees and a piece of honeycomb. Midgut and hindgut samples were taken after 7, 14 and 21 days and midgut without hindgut was taken after 21 days. Samples were homogenized and used for amylase zymogram, IEF and enzyme assays.
There was no mortality during the experiment. A high protein concentration was detected in the midgut in both groups of bees. Amylase activity was significantly higher in bees fed pollen enriched patties, which is shown by enzyme assay and by zymograms. There are different amylase isoforms present in bees fed by pollen enriched patties in comparison to bees fed by commercial patties, but
the major isoforms were the same.
The observed decrease in the amylase activity over time is probably due to dilution caused by the accumulation of water and undigested substances in the hindgut. Reduced amylase activity in the intestines of bees fed by commercial patties is due to a lack of starch or some other inducers present in pollen.
Because pollen is honey bee natural food, we conclude that food supplements that induce similar enzyme expression as pollen can be superior in comparison to supplements that induce very different enzyme expression.",
publisher = "Belgrade : University of Belgrade",
journal = "Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia",
title = "Expression of amylases in adult honey bees fed with different patties",
pages = "196",
url = "https://hdl.handle.net/21.15107/rcub_cer_5886"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Slomo, K., Ristović, M.,& Vujčić, Z.. (2021). Expression of amylases in adult honey bees fed with different patties. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia
Belgrade : University of Belgrade., 196.
https://hdl.handle.net/21.15107/rcub_cer_5886

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Slomo K, Ristović M, Vujčić Z. Expression of amylases in adult honey bees fed with different patties. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia. 2021;:196.
https://hdl.handle.net/21.15107/rcub_cer_5886 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Slomo, Katarina, Ristović, Marina, Vujčić, Zoran, "Expression of amylases in adult honey bees fed with different patties" in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade, Serbia (2021):196,
https://hdl.handle.net/21.15107/rcub_cer_5886 .

TY  - JOUR
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4540
AB  - There is an urgent need to increase the daily intake of insoluble dietary fiber, and at the same time to find new sources and new production technologies. We hypothesized that fungal enzymes directly involved in lignocellulosic material hydrolysis (Aspergillus and Trichoderma enzyme cocktails) will change the fiber structure particularly efficiently after the action of laccase (Trametes versicolor enzyme cocktail). Enzymes production on an inducing substrate (same as starting material for obtainment of insoluble dietary fibers) and their usage resulted in obtainment of novel insoluble dietary fibers with better characteristics, 24% higher swelling, 43% higher WRC and 57% higher ORC compared to insoluble dietary fibers from triticale (already proven to be a good food additive). Changes in structure were analyzed by FTIR and microscopic analysis. Antioxidative performance of the obtained products, new insoluble and released soluble dietary fibers, was analyzed in detail. Newly obtained soluble dietary fibers demonstrated up to 20 times higher antioxidant activity compared to untreated fibers (ABTS and DPPH tests). These results suggest their good performance as a future food additive. At the same time, they prove the hypothesis that the use of enzyme cocktails rich in laccase is a good choice for biological pretreatment in this process.
PB  - Elsevier
T2  - LWT - Food Science and Technology
T1  - Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale
VL  - 145
SP  - 111291
DO  - 10.1016/j.lwt.2021.111291
ER  - 

@article{
author = "Margetić, Aleksandra and Stojanović, Sanja and Ristović, Marina and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "There is an urgent need to increase the daily intake of insoluble dietary fiber, and at the same time to find new sources and new production technologies. We hypothesized that fungal enzymes directly involved in lignocellulosic material hydrolysis (Aspergillus and Trichoderma enzyme cocktails) will change the fiber structure particularly efficiently after the action of laccase (Trametes versicolor enzyme cocktail). Enzymes production on an inducing substrate (same as starting material for obtainment of insoluble dietary fibers) and their usage resulted in obtainment of novel insoluble dietary fibers with better characteristics, 24% higher swelling, 43% higher WRC and 57% higher ORC compared to insoluble dietary fibers from triticale (already proven to be a good food additive). Changes in structure were analyzed by FTIR and microscopic analysis. Antioxidative performance of the obtained products, new insoluble and released soluble dietary fibers, was analyzed in detail. Newly obtained soluble dietary fibers demonstrated up to 20 times higher antioxidant activity compared to untreated fibers (ABTS and DPPH tests). These results suggest their good performance as a future food additive. At the same time, they prove the hypothesis that the use of enzyme cocktails rich in laccase is a good choice for biological pretreatment in this process.",
publisher = "Elsevier",
journal = "LWT - Food Science and Technology",
title = "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale",
volume = "145",
pages = "111291",
doi = "10.1016/j.lwt.2021.111291"
}

Margetić, A., Stojanović, S., Ristović, M., Vujčić, Z.,& Dojnov, B.. (2021). Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale. in LWT - Food Science and Technology
Elsevier., 145, 111291.
https://doi.org/10.1016/j.lwt.2021.111291

Margetić A, Stojanović S, Ristović M, Vujčić Z, Dojnov B. Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale. in LWT - Food Science and Technology. 2021;145:111291.
doi:10.1016/j.lwt.2021.111291 .

Margetić, Aleksandra, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, Dojnov, Biljana, "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale" in LWT - Food Science and Technology, 145 (2021):111291,
https://doi.org/10.1016/j.lwt.2021.111291 . .

TY  - DATA
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4541
AB  - FTIR spectra of IDFT treatment with fungal enzymatic complex.
PB  - Elsevier
T2  - LWT - Food Science and Technology
T1  - The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale"
UR  - https://hdl.handle.net/21.15107/rcub_cer_4541
ER  - 

@misc{
author = "Margetić, Aleksandra and Stojanović, Sanja and Ristović, Marina and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "FTIR spectra of IDFT treatment with fungal enzymatic complex.",
publisher = "Elsevier",
journal = "LWT - Food Science and Technology",
title = "The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale"",
url = "https://hdl.handle.net/21.15107/rcub_cer_4541"
}

Margetić, A., Stojanović, S., Ristović, M., Vujčić, Z.,& Dojnov, B.. (2021). The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale". in LWT - Food Science and Technology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cer_4541

Margetić A, Stojanović S, Ristović M, Vujčić Z, Dojnov B. The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale". in LWT - Food Science and Technology. 2021;.
https://hdl.handle.net/21.15107/rcub_cer_4541 .

Margetić, Aleksandra, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, Dojnov, Biljana, "The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale"" in LWT - Food Science and Technology (2021),
https://hdl.handle.net/21.15107/rcub_cer_4541 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5815
AB  - Xylooligosaccharides (XOS) are prebiotic, functional food ingredients, with biological benefits
such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity.
Usually, XOS are produced from xylan by the combination of pre-treatment, enzymatic, chemical
and/or auto-hydrolytic methods. Fungal xylanases are the most suitable for XOS production. Xylan
rich agro-industrial wastes (corn cob is one of them) are used as a substrate for fungal xylanase
production and as start material for xylan extraction. In situ XOS production by fungal growth on
xylan rich medium represents an attractive and advantageous approach but insufficient described till
now. This method has many advantages over the others because bypasses the extraction and
purification of xylan and enzymes, it is environmentally friendly, low cost and time-consuming.
This study demonstrated A. tubingensis FAT35 has a great capacity for the synthesis of XOS using
corn cob as a substrate in solid state fermentation (SSF). Obtained XOS, during the fungal growth,
were characterized by TLC and HPLC. Significant antioxidant potential was shown by antioxidant
tests (ORAC, DPPH and FRAP). The obtained XOS are suitable to be a functional food additive
and are obtained in the simplest way that is both environmentally and economically suitable.
PB  - Belgrade : University of Belgrade
C3  - Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade
T1  - In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_cer_5815
ER  - 

@conference{
author = "Stojanović, Sanja and Ristović, Marina and Margetić, Aleksandra and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "Xylooligosaccharides (XOS) are prebiotic, functional food ingredients, with biological benefits
such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity.
Usually, XOS are produced from xylan by the combination of pre-treatment, enzymatic, chemical
and/or auto-hydrolytic methods. Fungal xylanases are the most suitable for XOS production. Xylan
rich agro-industrial wastes (corn cob is one of them) are used as a substrate for fungal xylanase
production and as start material for xylan extraction. In situ XOS production by fungal growth on
xylan rich medium represents an attractive and advantageous approach but insufficient described till
now. This method has many advantages over the others because bypasses the extraction and
purification of xylan and enzymes, it is environmentally friendly, low cost and time-consuming.
This study demonstrated A. tubingensis FAT35 has a great capacity for the synthesis of XOS using
corn cob as a substrate in solid state fermentation (SSF). Obtained XOS, during the fungal growth,
were characterized by TLC and HPLC. Significant antioxidant potential was shown by antioxidant
tests (ORAC, DPPH and FRAP). The obtained XOS are suitable to be a functional food additive
and are obtained in the simplest way that is both environmentally and economically suitable.",
publisher = "Belgrade : University of Belgrade",
journal = "Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade",
title = "In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_cer_5815"
}

Stojanović, S., Ristović, M., Margetić, A., Vujčić, Z.,& Dojnov, B.. (2021). In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade
Belgrade : University of Belgrade., 155.
https://hdl.handle.net/21.15107/rcub_cer_5815

Stojanović S, Ristović M, Margetić A, Vujčić Z, Dojnov B. In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade. 2021;:155.
https://hdl.handle.net/21.15107/rcub_cer_5815 .

Stojanović, Sanja, Ristović, Marina, Margetić, Aleksandra, Vujčić, Zoran, Dojnov, Biljana, "In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob" in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade (2021):155,
https://hdl.handle.net/21.15107/rcub_cer_5815 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Stepanović, Jelena
AU  - Margetić, Aleksandra
AU  - Duduk, Nataša
AU  - Dojnov, Biljana
AU  - Vujčić, Zoran
AU  - Duduk, Bojan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5816
AB  - Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.
PB  - Sociedade Portuguesa de Química
C3  - Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line
T1  - Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate
SP  - 172
UR  - https://hdl.handle.net/21.15107/rcub_cer_5816
ER  - 

@conference{
author = "Stojanović, Sanja and Stepanović, Jelena and Margetić, Aleksandra and Duduk, Nataša and Dojnov, Biljana and Vujčić, Zoran and Duduk, Bojan",
year = "2021",
abstract = "Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.",
publisher = "Sociedade Portuguesa de Química",
journal = "Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line",
title = "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate",
pages = "172",
url = "https://hdl.handle.net/21.15107/rcub_cer_5816"
}

Stojanović, S., Stepanović, J., Margetić, A., Duduk, N., Dojnov, B., Vujčić, Z.,& Duduk, B.. (2021). Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line
Sociedade Portuguesa de Química., 172.
https://hdl.handle.net/21.15107/rcub_cer_5816

Stojanović S, Stepanović J, Margetić A, Duduk N, Dojnov B, Vujčić Z, Duduk B. Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line. 2021;:172.
https://hdl.handle.net/21.15107/rcub_cer_5816 .

Stojanović, Sanja, Stepanović, Jelena, Margetić, Aleksandra, Duduk, Nataša, Dojnov, Biljana, Vujčić, Zoran, Duduk, Bojan, "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate" in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line (2021):172,
https://hdl.handle.net/21.15107/rcub_cer_5816 .

TY  - CONF
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5818
AB  - Wild-type microorganisms from the environment represent a wide source of potential 
enzyme producers. In order to determine whether an isolated microorganism produces an 
enzyme of interest, various screen tests have been developed. A new screening method 
for detection of endo and exo-xylanase activity including short time growth of fungal 
strains on a minimal medium containing xylan (inducible substrate) as a carbon source is 
developed and used for testing 58 fungal isolates from genus Aspergillus. The test is based 
on the diffusion of samples (fermentation extracts) in polyacrylamide gel incorporated by 
xylan. Endoxylanase activity is detected as enlightenment in the gel after staining of xylan 
with Congo Red. Exoxylanase activity was visualized as a precipitate after staining of 
reduction oligosaccharide ends with NBT. Selected isolate A. tubigensis was grown on 
SSF where corn cob served as an inducible substrate. In order to examine the influence of 
nitrogen sources on endoxylanase production and fungal growth, two sources (peptone and 
urea) were varied in 3 concentrations (1, 5 and 10 g/L). There were statistically significant 
differences in the obtained activities. The increase in activity compared to the screening 
medium was ~250 times. The obtained enzymes with high specific activity were further 
used for the production of xylooligosaccharides in high yield which showed that the 
selection of strain A. tubingensis was good.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production
SP  - 138
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_cer_5818
ER  - 

@conference{
author = "Ristović, Marina and Stojanović, Sanja and Margetić, Aleksandra and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "Wild-type microorganisms from the environment represent a wide source of potential 
enzyme producers. In order to determine whether an isolated microorganism produces an 
enzyme of interest, various screen tests have been developed. A new screening method 
for detection of endo and exo-xylanase activity including short time growth of fungal 
strains on a minimal medium containing xylan (inducible substrate) as a carbon source is 
developed and used for testing 58 fungal isolates from genus Aspergillus. The test is based 
on the diffusion of samples (fermentation extracts) in polyacrylamide gel incorporated by 
xylan. Endoxylanase activity is detected as enlightenment in the gel after staining of xylan 
with Congo Red. Exoxylanase activity was visualized as a precipitate after staining of 
reduction oligosaccharide ends with NBT. Selected isolate A. tubigensis was grown on 
SSF where corn cob served as an inducible substrate. In order to examine the influence of 
nitrogen sources on endoxylanase production and fungal growth, two sources (peptone and 
urea) were varied in 3 concentrations (1, 5 and 10 g/L). There were statistically significant 
differences in the obtained activities. The increase in activity compared to the screening 
medium was ~250 times. The obtained enzymes with high specific activity were further 
used for the production of xylooligosaccharides in high yield which showed that the 
selection of strain A. tubingensis was good.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production",
pages = "138-139",
url = "https://hdl.handle.net/21.15107/rcub_cer_5818"
}

Ristović, M., Stojanović, S., Margetić, A., Vujčić, Z.,& Dojnov, B.. (2021). Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 138-139.
https://hdl.handle.net/21.15107/rcub_cer_5818

Ristović M, Stojanović S, Margetić A, Vujčić Z, Dojnov B. Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:138-139.
https://hdl.handle.net/21.15107/rcub_cer_5818 .

Ristović, Marina, Stojanović, Sanja, Margetić, Aleksandra, Vujčić, Zoran, Dojnov, Biljana, "Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):138-139,
https://hdl.handle.net/21.15107/rcub_cer_5818 .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4850
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxida-tive response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1 ), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - MDPI
T2  - Antioksidants
T1  - Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3
VL  - 10
IS  - 11
SP  - 1719
DO  - 10.3390/antiox10111719
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
year = "2021",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxida-tive response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1 ), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
publisher = "MDPI",
journal = "Antioksidants",
title = "Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3",
volume = "10",
number = "11",
pages = "1719",
doi = "10.3390/antiox10111719"
}

Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3. in Antioksidants
MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719

Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3. in Antioksidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .

Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3" in Antioksidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .