The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production
Само за регистроване кориснике
2023
Аутори
Ristović, MarinaMargetić, Aleksandra
Stojanović, Sanja
Šokarda Slavić, Marinela
Božić, Nataša
Vujčić, Zoran
Dojnov, Biljana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitat...ion from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process.
Кључне речи:
waste utilization / antioxidants / bioactiveИзвор:
Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany, 2023, P2.002-Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
Институција/група
IHTMTY - CONF AU - Ristović, Marina AU - Margetić, Aleksandra AU - Stojanović, Sanja AU - Šokarda Slavić, Marinela AU - Božić, Nataša AU - Vujčić, Zoran AU - Dojnov, Biljana PY - 2023 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7135 AB - Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitation from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process. PB - Elsevier C3 - Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany T1 - The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production SP - P2.002 UR - https://hdl.handle.net/21.15107/rcub_cer_7135 ER -
@conference{ author = "Ristović, Marina and Margetić, Aleksandra and Stojanović, Sanja and Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana", year = "2023", abstract = "Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitation from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process.", publisher = "Elsevier", journal = "Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany", title = "The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production", pages = "P2.002", url = "https://hdl.handle.net/21.15107/rcub_cer_7135" }
Ristović, M., Margetić, A., Stojanović, S., Šokarda Slavić, M., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production. in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany Elsevier., P2.002. https://hdl.handle.net/21.15107/rcub_cer_7135
Ristović M, Margetić A, Stojanović S, Šokarda Slavić M, Božić N, Vujčić Z, Dojnov B. The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production. in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany. 2023;:P2.002. https://hdl.handle.net/21.15107/rcub_cer_7135 .
Ristović, Marina, Margetić, Aleksandra, Stojanović, Sanja, Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production" in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany (2023):P2.002, https://hdl.handle.net/21.15107/rcub_cer_7135 .