TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4850
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxida-tive response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1 ), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - MDPI
T2  - Antioksidants
T1  - Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3
VL  - 10
IS  - 11
SP  - 1719
DO  - 10.3390/antiox10111719
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
year = "2021",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxida-tive response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1 ), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
publisher = "MDPI",
journal = "Antioksidants",
title = "Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3",
volume = "10",
number = "11",
pages = "1719",
doi = "10.3390/antiox10111719"
}

Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3. in Antioksidants
MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719

Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3. in Antioksidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .

Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative responses of duckweed (Lemna minor l.) to phenol and rhizosphere-associated bacterial strain hafnia paralvei c32-106/3" in Antioksidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .

TY  - JOUR
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
AU  - Opsenica, Dejan
AU  - Gašić, Uroš
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2019.02287/abstract
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3093
AB  - European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and it is traditionally processed into slivovitz brandy. The annual plum yields in Serbia is strongly affected by Monilinia laxa, causing brown rot in stone fruits. The fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Lepotica and Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of 11 M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist, P4/16_1 was identified as Pseudomonas synxantha, which reduced mycelial growth of M. laxa from 80 to 87.5%. The production of volatile organic compounds was observed as well. Screening for the antibiotic coding gene was positive for phenazine-1-carboxylic acid, and benzene extraction of P. synxantha-active compounds was done. The crude benzene extract exhibited 57–63% inhibition of mycelial growth. The conducted LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for use as a biocontrol agent against brown rot-causing fungi.
PB  - Frontiers
T2  - Frontiers in Microbiology
T1  - Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa
VL  - 10
SP  - 2287
DO  - 10.3389/fmicb.2019.02287
ER  - 

@article{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević Grbić, Milica and Opsenica, Dejan and Gašić, Uroš and Stanković, Slaviša and Berić, Tanja",
year = "2019",
abstract = "European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and it is traditionally processed into slivovitz brandy. The annual plum yields in Serbia is strongly affected by Monilinia laxa, causing brown rot in stone fruits. The fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Lepotica and Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of 11 M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist, P4/16_1 was identified as Pseudomonas synxantha, which reduced mycelial growth of M. laxa from 80 to 87.5%. The production of volatile organic compounds was observed as well. Screening for the antibiotic coding gene was positive for phenazine-1-carboxylic acid, and benzene extraction of P. synxantha-active compounds was done. The crude benzene extract exhibited 57–63% inhibition of mycelial growth. The conducted LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for use as a biocontrol agent against brown rot-causing fungi.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology",
title = "Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa",
volume = "10",
pages = "2287",
doi = "10.3389/fmicb.2019.02287"
}

Janakiev, T., Dimkić, I., Unković, N., Ljaljević Grbić, M., Opsenica, D., Gašić, U., Stanković, S.,& Berić, T.. (2019). Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa. in Frontiers in Microbiology
Frontiers., 10, 2287.
https://doi.org/10.3389/fmicb.2019.02287

Janakiev T, Dimkić I, Unković N, Ljaljević Grbić M, Opsenica D, Gašić U, Stanković S, Berić T. Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa. in Frontiers in Microbiology. 2019;10:2287.
doi:10.3389/fmicb.2019.02287 .

Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević Grbić, Milica, Opsenica, Dejan, Gašić, Uroš, Stanković, Slaviša, Berić, Tanja, "Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa" in Frontiers in Microbiology, 10 (2019):2287,
https://doi.org/10.3389/fmicb.2019.02287 . .

TY  - JOUR
AU  - Unković, N
AU  - Dimkić, I
AU  - Stanković, Slaviša
AU  - Jelikić, A
AU  - Stanojević, D
AU  - Popović, Slađana
AU  - Stupar, M
AU  - Vukojević, J
AU  - Grbić, M L
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2928
AB  - The main purpose of this study was to isolate airborne fungi and assess seasonal variations in air contamination with their particulates by determining the levels of their propagules in the nave and exonarthex of a church. We also monitored indoor microclimate as a determining factor for fungal proliferation on wall paintings, spore release, and transmission through the air. The temperature and relative humidity of the nave favoured fungal growth. A total of 33 fungi were isolated, mainly of the phylum Ascomycota, and to the lesser extent of the phyla Zygomycota and Basidiomycota. The most common were the fungi of the genera Penicillium and Aspergillus (23.55 % and 20.58 %, respectively). Sørensen's quotient of similarity (0.37) suggests moderate species overlap and constant exchange of fungal propagules between the nave and exonarthex. The autumn had the highest diversity, with 17 documented taxa, followed by the summer and the winter. The spring had only eight taxa. Quantitative analysis of the airborne mycobiota in the nave (430±84.85 to 1880±106.07 CFU m -3 ) and exonarthex (715±59.62 to 2295±91.92 CFU m -3 ) showed very high contamination throughout the year, with values exceeding the maximum permissible concentrations by most standards. Many of the fungi determined in this study are known for their biodeteriogenic, toxigenic, and allergenic properties, and are a threat not only to occasional visitors and staff, but also to valuable works of art decorating nave walls. © 2018 Sciendo. All rights reserved. All Rights Reserved.
AB  - Glavni cilj ovoga istraživanja bio je izolirati gljive iz zraka i procijeniti sezonske promjene u onečišćenju zraka gljivičnim propagulama u naosu i egzonarteksu istraživane crkve. Također su praćeni mikroklimatski parametri kao ograničavajući čimbenici za razvoj i rast gljiva na zidnim slikama, za oslobađanje spora i njihovu transmisiju kroz zrak. Zabilježena temperatura i relativna vlažnost zraka u naosu pogodovale su razvoju i rastu gljiva. Dokumentirana su 33 taksona gljiva, uglavnom pripadnika koljena Ascomycota, a manje su zastupljeni oni Zygomycota i Basidiomycota. Najčešće su bile prisutne plijesni rodova Penicillium (23,55 %) i Aspergillus (20,58 %). Sørensenov indeks sličnosti (0,37) upućuje na stalnu i umjerenu razmjenu gljivičnih propagula između naosa i egzonarteksa. Uzorci uzeti u jesen pokazali su najveću raznolikost sa 17 zabilježenih taksona, a oni uzeti u proljeće samo osam taksona. Kvantitativna mikološka analiza u naosu (430±84,85 do 1880±106,07 CFU m-3) i egzonarteksu (715±59,62 do 2295±91,92 CFU m-3) pokazala je visoku godišnju razinu onečišćenja zraka, s vrijednostima koje prema većini standarda prelaze dopuštene koncentracije. Mnoge identificirane gljive mogu dovesti do biodeterioracije, proizvesti mikotoksine i izazvati alergijske reakcije. Stoga su nepoželjne u sakralnim objektima ne samo zbog očuvanja murala nego i zbog zaštite zdravlja zaposlenih, posjetitelja i konzervatora.
PB  - Sciendo
T2  - Arhiv za Higijenu Rada i Toksikologiju
T2  - Arhiv za Higijenu Rada i Toksikologiju
T1  - Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment
T1  - Sezonska raznovrsnost biodeteriogenih, patogenih i toksigenih gljiva u zraku sakralnoga objekta
VL  - 69
IS  - 4
SP  - 317
EP  - 327
DO  - 10.2478/aiht-2018-69-3194
ER  - 

@article{
author = "Unković, N and Dimkić, I and Stanković, Slaviša and Jelikić, A and Stanojević, D and Popović, Slađana and Stupar, M and Vukojević, J and Grbić, M L",
year = "2018",
abstract = "The main purpose of this study was to isolate airborne fungi and assess seasonal variations in air contamination with their particulates by determining the levels of their propagules in the nave and exonarthex of a church. We also monitored indoor microclimate as a determining factor for fungal proliferation on wall paintings, spore release, and transmission through the air. The temperature and relative humidity of the nave favoured fungal growth. A total of 33 fungi were isolated, mainly of the phylum Ascomycota, and to the lesser extent of the phyla Zygomycota and Basidiomycota. The most common were the fungi of the genera Penicillium and Aspergillus (23.55 % and 20.58 %, respectively). Sørensen's quotient of similarity (0.37) suggests moderate species overlap and constant exchange of fungal propagules between the nave and exonarthex. The autumn had the highest diversity, with 17 documented taxa, followed by the summer and the winter. The spring had only eight taxa. Quantitative analysis of the airborne mycobiota in the nave (430±84.85 to 1880±106.07 CFU m -3 ) and exonarthex (715±59.62 to 2295±91.92 CFU m -3 ) showed very high contamination throughout the year, with values exceeding the maximum permissible concentrations by most standards. Many of the fungi determined in this study are known for their biodeteriogenic, toxigenic, and allergenic properties, and are a threat not only to occasional visitors and staff, but also to valuable works of art decorating nave walls. © 2018 Sciendo. All rights reserved. All Rights Reserved., Glavni cilj ovoga istraživanja bio je izolirati gljive iz zraka i procijeniti sezonske promjene u onečišćenju zraka gljivičnim propagulama u naosu i egzonarteksu istraživane crkve. Također su praćeni mikroklimatski parametri kao ograničavajući čimbenici za razvoj i rast gljiva na zidnim slikama, za oslobađanje spora i njihovu transmisiju kroz zrak. Zabilježena temperatura i relativna vlažnost zraka u naosu pogodovale su razvoju i rastu gljiva. Dokumentirana su 33 taksona gljiva, uglavnom pripadnika koljena Ascomycota, a manje su zastupljeni oni Zygomycota i Basidiomycota. Najčešće su bile prisutne plijesni rodova Penicillium (23,55 %) i Aspergillus (20,58 %). Sørensenov indeks sličnosti (0,37) upućuje na stalnu i umjerenu razmjenu gljivičnih propagula između naosa i egzonarteksa. Uzorci uzeti u jesen pokazali su najveću raznolikost sa 17 zabilježenih taksona, a oni uzeti u proljeće samo osam taksona. Kvantitativna mikološka analiza u naosu (430±84,85 do 1880±106,07 CFU m-3) i egzonarteksu (715±59,62 do 2295±91,92 CFU m-3) pokazala je visoku godišnju razinu onečišćenja zraka, s vrijednostima koje prema većini standarda prelaze dopuštene koncentracije. Mnoge identificirane gljive mogu dovesti do biodeterioracije, proizvesti mikotoksine i izazvati alergijske reakcije. Stoga su nepoželjne u sakralnim objektima ne samo zbog očuvanja murala nego i zbog zaštite zdravlja zaposlenih, posjetitelja i konzervatora.",
publisher = "Sciendo",
journal = "Arhiv za Higijenu Rada i Toksikologiju, Arhiv za Higijenu Rada i Toksikologiju",
title = "Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment, Sezonska raznovrsnost biodeteriogenih, patogenih i toksigenih gljiva u zraku sakralnoga objekta",
volume = "69",
number = "4",
pages = "317-327",
doi = "10.2478/aiht-2018-69-3194"
}

Unković, N., Dimkić, I., Stanković, S., Jelikić, A., Stanojević, D., Popović, S., Stupar, M., Vukojević, J.,& Grbić, M. L.. (2018). Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment. in Arhiv za Higijenu Rada i Toksikologiju
Sciendo., 69(4), 317-327.
https://doi.org/10.2478/aiht-2018-69-3194

Unković N, Dimkić I, Stanković S, Jelikić A, Stanojević D, Popović S, Stupar M, Vukojević J, Grbić ML. Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment. in Arhiv za Higijenu Rada i Toksikologiju. 2018;69(4):317-327.
doi:10.2478/aiht-2018-69-3194 .

Unković, N, Dimkić, I, Stanković, Slaviša, Jelikić, A, Stanojević, D, Popović, Slađana, Stupar, M, Vukojević, J, Grbić, M L, "Seasonal diversity of biodeteriogenic, pathogenic, and toxigenic constituents of airborne mycobiota in a sacral environment" in Arhiv za Higijenu Rada i Toksikologiju, 69, no. 4 (2018):317-327,
https://doi.org/10.2478/aiht-2018-69-3194 . .

TY  - JOUR
AU  - Stević, Tatjana
AU  - Stanković, Slaviša
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Dimkić, Ivica
AU  - Soković, Marina
AU  - Berić, Tanja
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1454
AB  - In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses.
AB  - Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants
T1  - Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja
VL  - 34
IS  - 34
SP  - 69
EP  - 80
DO  - 10.5937/leksir1434069S
ER  - 

@article{
author = "Stević, Tatjana and Stanković, Slaviša and Šavikin, Katarina and Gođevac, Dejan and Dimkić, Ivica and Soković, Marina and Berić, Tanja",
year = "2014",
abstract = "In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses., Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants, Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja",
volume = "34",
number = "34",
pages = "69-80",
doi = "10.5937/leksir1434069S"
}

Stević, T., Stanković, S., Šavikin, K., Gođevac, D., Dimkić, I., Soković, M.,& Berić, T.. (2014). Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic"., 34(34), 69-80.
https://doi.org/10.5937/leksir1434069S

Stević T, Stanković S, Šavikin K, Gođevac D, Dimkić I, Soković M, Berić T. Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants. in Lekovite sirovine. 2014;34(34):69-80.
doi:10.5937/leksir1434069S .

Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, Berić, Tanja, "Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants" in Lekovite sirovine, 34, no. 34 (2014):69-80,
https://doi.org/10.5937/leksir1434069S . .