Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application
Samo za registrovane korisnike
2021
Autori
Đurđić, SlađanaStanković, Vesna
Vlahović, Filip
Ognjanović, Miloš
Kalcher, Kurt
Ćirković Veličković, T.
Mutić, Jelena
Stanković, Dalibor M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision... of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited.
Ključne reči:
Biosensors / Electrodes / Enzyme immobilization / Graphene / Graphene Nanoplatelets / Accuracy and precision / Amperometric response / Analytical applications / Detection limits / Electrochemical characteristics / Precise determinations / Screen printed carbon electrode (SPCEs) / Wide-linear range / Manganese oxideIzvor:
Journal of Electrochemical Society, 2021, 168, 3, 037510-Izdavač:
- The Electrochemical Society ("ECS").
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200168 (Univerzitet u Beogradu, Hemijski fakultet) (RS-MESTD-inst-2020-200168)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-MESTD-inst-2020-200026)
- CEEPUS network CIII-CZ-0212-13-1920-M-131892 (Education of Modern Analytical and Bioanalytical Methods)
- Eureka project call, E! 13303 MED-BIO-TEST
Napomena:
- The peer-review version: https://cer.ihtm.bg.ac.rs/handle/123456789/4578
Povezane informacije:
- Druga verzija
https://cer.ihtm.bg.ac.rs/handle/123456789/4578
DOI: 10.1149/1945-7111/abeaf2
ISSN: 0013-4651
WoS: 000629756800001
Scopus: 2-s2.0-85103235625
Institucija/grupa
IHTMTY - JOUR AU - Đurđić, Slađana AU - Stanković, Vesna AU - Vlahović, Filip AU - Ognjanović, Miloš AU - Kalcher, Kurt AU - Ćirković Veličković, T. AU - Mutić, Jelena AU - Stanković, Dalibor M. PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4523 AB - Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited. PB - The Electrochemical Society ("ECS"). T2 - Journal of Electrochemical Society T1 - Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application VL - 168 IS - 3 SP - 037510 DO - 10.1149/1945-7111/abeaf2 ER -
@article{ author = "Đurđić, Slađana and Stanković, Vesna and Vlahović, Filip and Ognjanović, Miloš and Kalcher, Kurt and Ćirković Veličković, T. and Mutić, Jelena and Stanković, Dalibor M.", year = "2021", abstract = "Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited.", publisher = "The Electrochemical Society ("ECS").", journal = "Journal of Electrochemical Society", title = "Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application", volume = "168", number = "3", pages = "037510", doi = "10.1149/1945-7111/abeaf2" }
Đurđić, S., Stanković, V., Vlahović, F., Ognjanović, M., Kalcher, K., Ćirković Veličković, T., Mutić, J.,& Stanković, D. M.. (2021). Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. in Journal of Electrochemical Society The Electrochemical Society ("ECS").., 168(3), 037510. https://doi.org/10.1149/1945-7111/abeaf2
Đurđić S, Stanković V, Vlahović F, Ognjanović M, Kalcher K, Ćirković Veličković T, Mutić J, Stanković DM. Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. in Journal of Electrochemical Society. 2021;168(3):037510. doi:10.1149/1945-7111/abeaf2 .
Đurđić, Slađana, Stanković, Vesna, Vlahović, Filip, Ognjanović, Miloš, Kalcher, Kurt, Ćirković Veličković, T., Mutić, Jelena, Stanković, Dalibor M., "Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application" in Journal of Electrochemical Society, 168, no. 3 (2021):037510, https://doi.org/10.1149/1945-7111/abeaf2 . .