Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application
Authors
Đurđić, Slađana
Stanković, Vesna

Vlahović, Filip

Ognjanović, Miloš

Kalcher, Kurt
Ćirković Veličković, T.
Mutić, Jelena

Stanković, Dalibor M.

Article (Accepted Version)

Metadata
Show full item recordAbstract
Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision... of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited.
Keywords:
Biosensors / Electrodes / Enzyme immobilization / Graphene / Graphene Nanoplatelets / Accuracy and precision / Amperometric response / Analytical applications / Detection limits / Electrochemical characteristics / Precise determinations / Screen printed carbon electrode (SPCEs) / Wide-linear range / Manganese oxideSource:
Journal of Electrochemical Society, 2021, 168, 3, 037510-Publisher:
- The Electrochemical Society ("ECS").
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
- CEEPUS network CIII-CZ-0212-13-1920-M-131892 (Education of Modern Analytical and Bioanalytical Methods)
- Eureka project call, E! 13303 MED-BIO-TEST
Note:
- This is the peer-reviewed version of the article: Đurđić, S., Stanković, V., Vlahović, F., Ognjanović, M., Kalcher, K., Ćirković Veličković, T., Mutić, J. J.,& Stanković, D. M. (2021). Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. Journal of Electrochemical Society, 168(3), 037510. https://doi.org/10.1149/1945-7111/abeaf2
- Published version: https://cer.ihtm.bg.ac.rs/handle/123456789/4523
Related info:
- Version of
https://doi.org/10.1149/1945-7111/abeaf2 - Version of
https://cer.ihtm.bg.ac.rs/handle/123456789/4523
DOI: 10.1149/1945-7111/abeaf2
ISSN: 0013-4651
WoS: 000629756800001
Scopus: 2-s2.0-85103235625
Collections
Institution/Community
IHTMTY - JOUR AU - Đurđić, Slađana AU - Stanković, Vesna AU - Vlahović, Filip AU - Ognjanović, Miloš AU - Kalcher, Kurt AU - Ćirković Veličković, T. AU - Mutić, Jelena AU - Stanković, Dalibor M. PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4578 AB - Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited. PB - The Electrochemical Society ("ECS"). T2 - Journal of Electrochemical Society T1 - Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application VL - 168 IS - 3 SP - 037510 DO - 10.1149/1945-7111/abeaf2 ER -
@article{ author = "Đurđić, Slađana and Stanković, Vesna and Vlahović, Filip and Ognjanović, Miloš and Kalcher, Kurt and Ćirković Veličković, T. and Mutić, Jelena and Stanković, Dalibor M.", year = "2021", abstract = "Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited.", publisher = "The Electrochemical Society ("ECS").", journal = "Journal of Electrochemical Society", title = "Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application", volume = "168", number = "3", pages = "037510", doi = "10.1149/1945-7111/abeaf2" }
Đurđić, S., Stanković, V., Vlahović, F., Ognjanović, M., Kalcher, K., Ćirković Veličković, T., Mutić, J.,& Stanković, D. M.. (2021). Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. in Journal of Electrochemical Society The Electrochemical Society ("ECS").., 168(3), 037510. https://doi.org/10.1149/1945-7111/abeaf2
Đurđić S, Stanković V, Vlahović F, Ognjanović M, Kalcher K, Ćirković Veličković T, Mutić J, Stanković DM. Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. in Journal of Electrochemical Society. 2021;168(3):037510. doi:10.1149/1945-7111/abeaf2 .
Đurđić, Slađana, Stanković, Vesna, Vlahović, Filip, Ognjanović, Miloš, Kalcher, Kurt, Ćirković Veličković, T., Mutić, Jelena, Stanković, Dalibor M., "Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application" in Journal of Electrochemical Society, 168, no. 3 (2021):037510, https://doi.org/10.1149/1945-7111/abeaf2 . .