dc.creator | Medić, Ana | |
dc.creator | Stojanović, Ksenija | |
dc.creator | Izrael-Živković, Lidija | |
dc.creator | Beškoski, Vladimir | |
dc.creator | Lončarević, Branka | |
dc.creator | Kazazić, Saša | |
dc.creator | Karadžić, Ivanka | |
dc.date.accessioned | 2019-11-23T18:09:51Z | |
dc.date.available | 2019-11-23T18:09:51Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2046-2069 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/3214 | |
dc.description.abstract | The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This
investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size,
pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the
pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and
100 mg L 1
, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme
preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal
than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of
aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data,
a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was
conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the
upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests;
therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A
comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to
aromatic substances can be summarized as the upregulation of proteins responsible for amino acid
metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes
of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic
compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated
areas | en |
dc.language.iso | en | sr |
dc.publisher | Royal Society of Chemistry | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/176006/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | RSC Advances | sr |
dc.subject | 2,6-di-tert-butylphenol | sr |
dc.subject | Pseudomonas aeruginosa san ai | sr |
dc.subject | plastic additives | sr |
dc.subject | degradation | sr |
dc.subject | decontamination | sr |
dc.title | A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai | en |
dc.type | article | sr |
dc.rights.license | BY | sr |
dcterms.abstract | Караджић, Иванка; Казазић, Саша; Медић, Aна; Лончаревић, Бранка; Бешкоски, Владимир; Израел-Живковић, Лидија; Стојановић, Ксенија; | |
dc.citation.volume | 9 | |
dc.citation.issue | 41 | |
dc.citation.spage | 23696 | |
dc.citation.epage | 23710 | |
dc.citation.rank | M22~ | |
dc.identifier.doi | 10.1039/c9ra04298a | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs/bitstream/id/15428/c9ra04298a.pdf | |
dc.identifier.scopus | 2-s2.0-85070413922 | |
dc.identifier.wos | 000478947000030 | |
dc.type.version | publishedVersion | sr |