A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai
Authors
Medić, Ana
Stojanović, Ksenija

Izrael-Živković, Lidija
Beškoski, Vladimir

Lončarević, Branka

Kazazić, Saša
Karadžić, Ivanka

Article (Published version)
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The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This
investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size,
pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the
pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and
100 mg L 1
, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme
preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal
than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of
aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data,
a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was
conducted by me...ans of nanoLC-MS/MS. The presence of aromatic substances resulted in the
upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests;
therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A
comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to
aromatic substances can be summarized as the upregulation of proteins responsible for amino acid
metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes
of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic
compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated
areas
Keywords:
2,6-di-tert-butylphenol / Pseudomonas aeruginosa san ai / plastic additives / degradation / decontaminationSource:
RSC Advances, 2019, 9, 41, 23696-23710Publisher:
- Royal Society of Chemistry
Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
- Geochemical investigations of sedimentary rocks - fossil fuels and environmental pollutants (RS-176006)
DOI: 10.1039/c9ra04298a
ISSN: 2046-2069
WoS: 000478947000030
Scopus: 2-s2.0-85070413922
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Institution/Community
IHTMTY - JOUR AU - Medić, Ana AU - Stojanović, Ksenija AU - Izrael-Živković, Lidija AU - Beškoski, Vladimir AU - Lončarević, Branka AU - Kazazić, Saša AU - Karadžić, Ivanka PY - 2019 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3214 AB - The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L 1 , the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas PB - Royal Society of Chemistry T2 - RSC Advances T1 - A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai VL - 9 IS - 41 SP - 23696 EP - 23710 DO - 10.1039/c9ra04298a ER -
@article{ author = "Medić, Ana and Stojanović, Ksenija and Izrael-Živković, Lidija and Beškoski, Vladimir and Lončarević, Branka and Kazazić, Saša and Karadžić, Ivanka", year = "2019", abstract = "The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L 1 , the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas", publisher = "Royal Society of Chemistry", journal = "RSC Advances", title = "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai", volume = "9", number = "41", pages = "23696-23710", doi = "10.1039/c9ra04298a" }
Medić, A., Stojanović, K., Izrael-Živković, L., Beškoski, V., Lončarević, B., Kazazić, S.,& Karadžić, I.. (2019). A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances Royal Society of Chemistry., 9(41), 23696-23710. https://doi.org/10.1039/c9ra04298a
Medić A, Stojanović K, Izrael-Živković L, Beškoski V, Lončarević B, Kazazić S, Karadžić I. A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances. 2019;9(41):23696-23710. doi:10.1039/c9ra04298a .
Medić, Ana, Stojanović, Ksenija, Izrael-Živković, Lidija, Beškoski, Vladimir, Lončarević, Branka, Kazazić, Saša, Karadžić, Ivanka, "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai" in RSC Advances, 9, no. 41 (2019):23696-23710, https://doi.org/10.1039/c9ra04298a . .