Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells
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2015
Authors
Stefanović, IvanĐonlagić, Jasna
Tovilović, Gordana
Nestorov, Jelena
Antić, Vesna
Ostojić, Sanja
Pergal, Marija
Article (Published version)
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Polyurethane copolymers based on ,-dihydroxypropyl poly(dimethylsiloxane) (PDMS) with a range of soft segment contents were prepared by two-stage polymerization, and their microstructures, thermal, thermomechanical, and surface properties, as well as in vitro hemo- and cytocompatibility were evaluated. All utilized characterization methods confirmed the existence of moderately microphase separated structures with the appearance of some microphase mixing between segments as the PDMS (i.e., soft segment) content increased. Copolymers showed higher crystallinity, storage moduli, surface roughness, and surface free energy, but less hydrophobicity with decreasing PDMS content. Biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact, an extraction method and after pretreatment of copolymers with multicomponent protein mixture, as well as by a competitive protein adsorption assay. Copolymers showed no toxic effect to endothelial cells and all cop...olymers, except that with the lowest PDMS content, exhibited resistance to endothelial cell adhesion, suggesting their unsuitability for long-term biomedical devices which particularly require re-endothelialization. All copolymers exhibited excellent resistance to fibrinogen adsorption and adsorbed more albumin than fibrinogen in the competitive adsorption assay, suggesting their good hemocompatibility. The noncytotoxic chemistry of these synthesized materials, combined with their nonadherent properties which are inhospitable to cell attachment and growth, underlie the need for further investigations to clarify their potential for use in short-term biomedical devices.
Keywords:
poly(urethane-dimethylsiloxane)s / biocompatibility / cytotoxicity / endothelial cells / competitive protein adsorptionSource:
Journal of Biomedical Materials Research Part A, 2015, 103, 4, 1459-1475Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.1002/jbm.a.35285
ISSN: 1549-3296
PubMed: 25046378
WoS: 000350395300017
Scopus: 2-s2.0-84923537161
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IHTMTY - JOUR AU - Stefanović, Ivan AU - Đonlagić, Jasna AU - Tovilović, Gordana AU - Nestorov, Jelena AU - Antić, Vesna AU - Ostojić, Sanja AU - Pergal, Marija PY - 2015 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1779 AB - Polyurethane copolymers based on ,-dihydroxypropyl poly(dimethylsiloxane) (PDMS) with a range of soft segment contents were prepared by two-stage polymerization, and their microstructures, thermal, thermomechanical, and surface properties, as well as in vitro hemo- and cytocompatibility were evaluated. All utilized characterization methods confirmed the existence of moderately microphase separated structures with the appearance of some microphase mixing between segments as the PDMS (i.e., soft segment) content increased. Copolymers showed higher crystallinity, storage moduli, surface roughness, and surface free energy, but less hydrophobicity with decreasing PDMS content. Biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact, an extraction method and after pretreatment of copolymers with multicomponent protein mixture, as well as by a competitive protein adsorption assay. Copolymers showed no toxic effect to endothelial cells and all copolymers, except that with the lowest PDMS content, exhibited resistance to endothelial cell adhesion, suggesting their unsuitability for long-term biomedical devices which particularly require re-endothelialization. All copolymers exhibited excellent resistance to fibrinogen adsorption and adsorbed more albumin than fibrinogen in the competitive adsorption assay, suggesting their good hemocompatibility. The noncytotoxic chemistry of these synthesized materials, combined with their nonadherent properties which are inhospitable to cell attachment and growth, underlie the need for further investigations to clarify their potential for use in short-term biomedical devices. PB - Wiley-Blackwell, Hoboken T2 - Journal of Biomedical Materials Research Part A T1 - Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells VL - 103 IS - 4 SP - 1459 EP - 1475 DO - 10.1002/jbm.a.35285 ER -
@article{ author = "Stefanović, Ivan and Đonlagić, Jasna and Tovilović, Gordana and Nestorov, Jelena and Antić, Vesna and Ostojić, Sanja and Pergal, Marija", year = "2015", abstract = "Polyurethane copolymers based on ,-dihydroxypropyl poly(dimethylsiloxane) (PDMS) with a range of soft segment contents were prepared by two-stage polymerization, and their microstructures, thermal, thermomechanical, and surface properties, as well as in vitro hemo- and cytocompatibility were evaluated. All utilized characterization methods confirmed the existence of moderately microphase separated structures with the appearance of some microphase mixing between segments as the PDMS (i.e., soft segment) content increased. Copolymers showed higher crystallinity, storage moduli, surface roughness, and surface free energy, but less hydrophobicity with decreasing PDMS content. Biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact, an extraction method and after pretreatment of copolymers with multicomponent protein mixture, as well as by a competitive protein adsorption assay. Copolymers showed no toxic effect to endothelial cells and all copolymers, except that with the lowest PDMS content, exhibited resistance to endothelial cell adhesion, suggesting their unsuitability for long-term biomedical devices which particularly require re-endothelialization. All copolymers exhibited excellent resistance to fibrinogen adsorption and adsorbed more albumin than fibrinogen in the competitive adsorption assay, suggesting their good hemocompatibility. The noncytotoxic chemistry of these synthesized materials, combined with their nonadherent properties which are inhospitable to cell attachment and growth, underlie the need for further investigations to clarify their potential for use in short-term biomedical devices.", publisher = "Wiley-Blackwell, Hoboken", journal = "Journal of Biomedical Materials Research Part A", title = "Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells", volume = "103", number = "4", pages = "1459-1475", doi = "10.1002/jbm.a.35285" }
Stefanović, I., Đonlagić, J., Tovilović, G., Nestorov, J., Antić, V., Ostojić, S.,& Pergal, M.. (2015). Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells. in Journal of Biomedical Materials Research Part A Wiley-Blackwell, Hoboken., 103(4), 1459-1475. https://doi.org/10.1002/jbm.a.35285
Stefanović I, Đonlagić J, Tovilović G, Nestorov J, Antić V, Ostojić S, Pergal M. Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells. in Journal of Biomedical Materials Research Part A. 2015;103(4):1459-1475. doi:10.1002/jbm.a.35285 .
Stefanović, Ivan, Đonlagić, Jasna, Tovilović, Gordana, Nestorov, Jelena, Antić, Vesna, Ostojić, Sanja, Pergal, Marija, "Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells" in Journal of Biomedical Materials Research Part A, 103, no. 4 (2015):1459-1475, https://doi.org/10.1002/jbm.a.35285 . .