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dc.creatorStefanović, Ivan
dc.creatorĐonlagić, Jasna
dc.creatorTovilović, Gordana
dc.creatorNestorov, Jelena
dc.creatorAntić, Vesna
dc.creatorOstojić, Sanja
dc.creatorPergal, Marija
dc.date.accessioned2019-01-30T17:46:50Z
dc.date.available2019-01-30T17:46:50Z
dc.date.issued2015
dc.identifier.issn1549-3296
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/1779
dc.description.abstractPolyurethane copolymers based on ,-dihydroxypropyl poly(dimethylsiloxane) (PDMS) with a range of soft segment contents were prepared by two-stage polymerization, and their microstructures, thermal, thermomechanical, and surface properties, as well as in vitro hemo- and cytocompatibility were evaluated. All utilized characterization methods confirmed the existence of moderately microphase separated structures with the appearance of some microphase mixing between segments as the PDMS (i.e., soft segment) content increased. Copolymers showed higher crystallinity, storage moduli, surface roughness, and surface free energy, but less hydrophobicity with decreasing PDMS content. Biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact, an extraction method and after pretreatment of copolymers with multicomponent protein mixture, as well as by a competitive protein adsorption assay. Copolymers showed no toxic effect to endothelial cells and all copolymers, except that with the lowest PDMS content, exhibited resistance to endothelial cell adhesion, suggesting their unsuitability for long-term biomedical devices which particularly require re-endothelialization. All copolymers exhibited excellent resistance to fibrinogen adsorption and adsorbed more albumin than fibrinogen in the competitive adsorption assay, suggesting their good hemocompatibility. The noncytotoxic chemistry of these synthesized materials, combined with their nonadherent properties which are inhospitable to cell attachment and growth, underlie the need for further investigations to clarify their potential for use in short-term biomedical devices.en
dc.publisherWiley-Blackwell, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172062/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Biomedical Materials Research Part A
dc.subjectpoly(urethane-dimethylsiloxane)sen
dc.subjectbiocompatibilityen
dc.subjectcytotoxicityen
dc.subjectendothelial cellsen
dc.subjectcompetitive protein adsorptionen
dc.titlePoly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДјонлагиц, Јасна; Несторов, Јелена; Aнтиц, Весна В; Остојиц, Сања; Пергал, Марија; Стефановић, Иван; Товиловиц, Гордана;
dc.citation.volume103
dc.citation.issue4
dc.citation.spage1459
dc.citation.epage1475
dc.citation.other103(4): 1459-1475
dc.citation.rankM21
dc.identifier.pmid25046378
dc.identifier.doi10.1002/jbm.a.35285
dc.identifier.rcubConv_3301
dc.identifier.scopus2-s2.0-84923537161
dc.identifier.wos000350395300017
dc.type.versionpublishedVersion


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