TY  - JOUR
AU  - Marković, Maja
AU  - Panić, Vesna
AU  - Šešlija, Sanja
AU  - Milivojević, Ana D.
AU  - Spasojević, Pavle
AU  - Bošković-Vragolović, Nevenka
AU  - Pjanović, Rada
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3604
AB  - Carriers for targeted delivery and controlled release of poorly water-soluble active
substances (PWSAS) are facing three challenges: (a) the encapsulation issues, (b)
limitations of PWSAS water solubility, and (c) burst drug release which can be
pharmacologically dangerous and economically inefficient. The present study
brings a novel strategy for encapsulation and controlled release of PWSAS—
caffeine in concentrations which are higher than its maximal water solubility
without the possibility of burst effect. The modification of hydrophilic carrier
based on poly(methacylic acid) was done using casein and liposomes. To further
increase the maximal caffeine loading inside the carrier nicotinamide was used.
The release study of the encapsulated PWSAS was elaborated with respect to morphology
of the carriers and interactions that could be established between its
structural components. The carriers swelling and the release of caffeine and nicotinamide
were also investigated depending on caffeine concentration, the presence
of different liposomal formulations and the volume ratio of liposomal formulation,
in three media with different pH simulating the path of the carrier through the
human gastrointestinal tract. The synthesized carriers are promising candidates
for encapsulation of PWSAS in concentrations which are higher than its maximal
water solubility and for the targeted delivery of those dosages.
PB  - Wiley
T2  - Polymer Engineering & Science
T1  - Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances
VL  - 60
SP  - 2008
EP  - 2022
DO  - 10.1002/pen.25448
ER  - 

@article{
author = "Marković, Maja and Panić, Vesna and Šešlija, Sanja and Milivojević, Ana D. and Spasojević, Pavle and Bošković-Vragolović, Nevenka and Pjanović, Rada",
year = "2020",
abstract = "Carriers for targeted delivery and controlled release of poorly water-soluble active
substances (PWSAS) are facing three challenges: (a) the encapsulation issues, (b)
limitations of PWSAS water solubility, and (c) burst drug release which can be
pharmacologically dangerous and economically inefficient. The present study
brings a novel strategy for encapsulation and controlled release of PWSAS—
caffeine in concentrations which are higher than its maximal water solubility
without the possibility of burst effect. The modification of hydrophilic carrier
based on poly(methacylic acid) was done using casein and liposomes. To further
increase the maximal caffeine loading inside the carrier nicotinamide was used.
The release study of the encapsulated PWSAS was elaborated with respect to morphology
of the carriers and interactions that could be established between its
structural components. The carriers swelling and the release of caffeine and nicotinamide
were also investigated depending on caffeine concentration, the presence
of different liposomal formulations and the volume ratio of liposomal formulation,
in three media with different pH simulating the path of the carrier through the
human gastrointestinal tract. The synthesized carriers are promising candidates
for encapsulation of PWSAS in concentrations which are higher than its maximal
water solubility and for the targeted delivery of those dosages.",
publisher = "Wiley",
journal = "Polymer Engineering & Science",
title = "Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances",
volume = "60",
pages = "2008-2022",
doi = "10.1002/pen.25448"
}

Marković, M., Panić, V., Šešlija, S., Milivojević, A. D., Spasojević, P., Bošković-Vragolović, N.,& Pjanović, R.. (2020). Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances. in Polymer Engineering & Science
Wiley., 60, 2008-2022.
https://doi.org/10.1002/pen.25448

Marković M, Panić V, Šešlija S, Milivojević AD, Spasojević P, Bošković-Vragolović N, Pjanović R. Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances. in Polymer Engineering & Science. 2020;60:2008-2022.
doi:10.1002/pen.25448 .

Marković, Maja, Panić, Vesna, Šešlija, Sanja, Milivojević, Ana D., Spasojević, Pavle, Bošković-Vragolović, Nevenka, Pjanović, Rada, "Novel strategy for encapsulation and targeted delivery of poorly water-soluble active substances" in Polymer Engineering & Science, 60 (2020):2008-2022,
https://doi.org/10.1002/pen.25448 . .

TY  - JOUR
AU  - Marković, Maja
AU  - Panić, Vesna
AU  - Šešlija, Sanja
AU  - Spasojević, Pavle
AU  - Ugrinović, Vukašin
AU  - Bošković-Vragolović, Nevenka
AU  - Pjanović, Rada
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3641
AB  - pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly water-soluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.
PB  - John Wiley and Sons Inc
T2  - Polymer Engineering and Science
T1  - Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance
VL  - 60
SP  - 2496
EP  - 2510
DO  - 10.1002/pen.25487
ER  - 

@article{
author = "Marković, Maja and Panić, Vesna and Šešlija, Sanja and Spasojević, Pavle and Ugrinović, Vukašin and Bošković-Vragolović, Nevenka and Pjanović, Rada",
year = "2020",
abstract = "pH sensitive, nontoxic, and biocompatible poly(methacrylic) acid (PMAA) based soft networks have been extensively used in the design of systems for targeted drug delivery. Still, their highly hydrophilic nature limits their potential to be used as a carrier of poorly water-soluble substances. With the aim to overcome this limitation, the present study details a new approach for modification of PMAA based carriers using two amphiphilic components: casein and liposomes. The FTIR analysis revealed structural features of each component as well as the synergetic effect that originated from the formation of specific interactions. Namely, hydrophobic interactions between the poorly water-soluble model drug (caffeine) and casein enabled caffeine encapsulation and controlled release, while addition of liposomes ensured better control of the release rate. The morphological properties of the carriers, swelling behavior, and release kinetics of caffeine were investigated depending on the variable synthesis parameters (neutralization degree of methacrylic acid, concentration of caffeine, presence/absence of liposomes) in two different media simulating the pH environment of human intestines and stomach. The data obtained from in vitro caffeine release were correlated and analyzed in detail using several mathematical models, indicating significant potential of investigated carriers for targeted delivery and controlled release of poorly water-soluble substances.",
publisher = "John Wiley and Sons Inc",
journal = "Polymer Engineering and Science",
title = "Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance",
volume = "60",
pages = "2496-2510",
doi = "10.1002/pen.25487"
}

Marković, M., Panić, V., Šešlija, S., Spasojević, P., Ugrinović, V., Bošković-Vragolović, N.,& Pjanović, R.. (2020). Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance. in Polymer Engineering and Science
John Wiley and Sons Inc., 60, 2496-2510.
https://doi.org/10.1002/pen.25487

Marković M, Panić V, Šešlija S, Spasojević P, Ugrinović V, Bošković-Vragolović N, Pjanović R. Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance. in Polymer Engineering and Science. 2020;60:2496-2510.
doi:10.1002/pen.25487 .

Marković, Maja, Panić, Vesna, Šešlija, Sanja, Spasojević, Pavle, Ugrinović, Vukašin, Bošković-Vragolović, Nevenka, Pjanović, Rada, "Modification of hydrophilic polymer network to design a carrier for a poorly water-soluble substance" in Polymer Engineering and Science, 60 (2020):2496-2510,
https://doi.org/10.1002/pen.25487 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Jakovljević, Dragica
AU  - Jadranin, Milka
AU  - Nikšić, Miomir
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3727
AB  - The health promoting effects of hot water extracts obtained from
fruiting bodies of the commercially cultivated mushroom Agaricus bisporus
(AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW)
originating from northern Serbia are presented in this research. These abilities
were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic
acid model system, inhibition of the angiotension converting I enzyme
(ACE) that could help in the maintenance of a normal blood pressure level and
strengthening the ability of the central cholinergic neuron by inhibiting the activity
of acetylcholinesterase (AChE). Cytotoxic activities were observed
towards selected human malignant (HeLa and K562) cell lines and normal-
-human peripheral blood mononuclear cells (PBMC). GrHW contains higher
phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE
(IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant
selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1)
and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein
(6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1)
contents and induced significant proliferation of healthy PBMC from 152–116
% in the concentration range of 0.047–0.187 mg mL-1. The difference in the
biological activity of the extracts provides guidance on their use as functional
food.
AB  - У овоме раду поређен је здраствени ефекaт врелих водених екстраката добијених из плодоносних тела комерцијално узгајане јестиве гљиве Agaricus bisporus (AbHW) и самоникле врсте гљиве Ganoderma resinaceum (GrHW) из региона северне Србије. Здраствени ефекат је поређен in vitro превенцијом липидне пероксидације (LPx) у модел систему линолеинске киселине, инхибицијом ангиотензин конвертујућег ензима (ACE) који има улогу у одржавању нормалног нивоа крвног притиска и јачањем способности централних холинергичких неурона инхибицијом активности ацетилхолинестеразе (AChE).
Цитотоксична активност је праћена на хуманим ћелијама тумора грлића материц (HeLa) и ћелијама хроничне мијелоидне леукемије (K562), као и на здравим мононуклеарним ћелијама периферног крвотока (PBMC). GrHW је показао већи садржај фенолних компоненти (5,9 g (100 g)-1), већу способност инхибиције LPx (EC50 = 1,07 mg mL-1),
ACE (IC50 = 0,54 mg mL-1) и AChE (IC50 = 0,37 mg mL-1); показао је већу селективност у антитуморском дејству према HeLa (IC50 = 0,14 mg mL-1) и K562 (IC50 = 0,11 mg mL-1) ћелијама. AbHW је показао већи укупни садржај протеина (6,4 g (100 g)-1), угљених хидрата (75,4 g (100 g)-1) и β-глукана (55,1 g (100 g)-1) и значајно je стимулисао пролиферацију PBMC ћелија од 152–116 % у распону концентрација од 0,046–0,187 mg mL-1. Разлика у биолошкој активности екстраката даје смернице у њиховој примени као функционалнe хранe.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview
T1  - Компаративни прглед здравствених ефеката комерцијално узгајане гљиве Agaricus bisporus и самоникле врсте гливе Ganoderma resinaceum
VL  - 85
IS  - 6
SP  - 721
EP  - 735
DO  - 10.2298/JSC190930129K
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Vunduk, Jovana and Jakovljević, Dragica and Jadranin, Milka and Nikšić, Miomir",
year = "2020",
abstract = "The health promoting effects of hot water extracts obtained from
fruiting bodies of the commercially cultivated mushroom Agaricus bisporus
(AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW)
originating from northern Serbia are presented in this research. These abilities
were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic
acid model system, inhibition of the angiotension converting I enzyme
(ACE) that could help in the maintenance of a normal blood pressure level and
strengthening the ability of the central cholinergic neuron by inhibiting the activity
of acetylcholinesterase (AChE). Cytotoxic activities were observed
towards selected human malignant (HeLa and K562) cell lines and normal-
-human peripheral blood mononuclear cells (PBMC). GrHW contains higher
phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE
(IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant
selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1)
and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein
(6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1)
contents and induced significant proliferation of healthy PBMC from 152–116
% in the concentration range of 0.047–0.187 mg mL-1. The difference in the
biological activity of the extracts provides guidance on their use as functional
food., У овоме раду поређен је здраствени ефекaт врелих водених екстраката добијених из плодоносних тела комерцијално узгајане јестиве гљиве Agaricus bisporus (AbHW) и самоникле врсте гљиве Ganoderma resinaceum (GrHW) из региона северне Србије. Здраствени ефекат је поређен in vitro превенцијом липидне пероксидације (LPx) у модел систему линолеинске киселине, инхибицијом ангиотензин конвертујућег ензима (ACE) који има улогу у одржавању нормалног нивоа крвног притиска и јачањем способности централних холинергичких неурона инхибицијом активности ацетилхолинестеразе (AChE).
Цитотоксична активност је праћена на хуманим ћелијама тумора грлића материц (HeLa) и ћелијама хроничне мијелоидне леукемије (K562), као и на здравим мононуклеарним ћелијама периферног крвотока (PBMC). GrHW је показао већи садржај фенолних компоненти (5,9 g (100 g)-1), већу способност инхибиције LPx (EC50 = 1,07 mg mL-1),
ACE (IC50 = 0,54 mg mL-1) и AChE (IC50 = 0,37 mg mL-1); показао је већу селективност у антитуморском дејству према HeLa (IC50 = 0,14 mg mL-1) и K562 (IC50 = 0,11 mg mL-1) ћелијама. AbHW је показао већи укупни садржај протеина (6,4 g (100 g)-1), угљених хидрата (75,4 g (100 g)-1) и β-глукана (55,1 g (100 g)-1) и значајно je стимулисао пролиферацију PBMC ћелија од 152–116 % у распону концентрација од 0,046–0,187 mg mL-1. Разлика у биолошкој активности екстраката даје смернице у њиховој примени као функционалнe хранe.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview, Компаративни прглед здравствених ефеката комерцијално узгајане гљиве Agaricus bisporus и самоникле врсте гливе Ganoderma resinaceum",
volume = "85",
number = "6",
pages = "721-735",
doi = "10.2298/JSC190930129K"
}

Kozarski, M. S., Klaus, A., Vunduk, J., Jakovljević, D., Jadranin, M.,& Nikšić, M.. (2020). Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 85(6), 721-735.
https://doi.org/10.2298/JSC190930129K

Kozarski MS, Klaus A, Vunduk J, Jakovljević D, Jadranin M, Nikšić M. Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview. in Journal of the Serbian Chemical Society. 2020;85(6):721-735.
doi:10.2298/JSC190930129K .

Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Jakovljević, Dragica, Jadranin, Milka, Nikšić, Miomir, "Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum – A comparative overview" in Journal of the Serbian Chemical Society, 85, no. 6 (2020):721-735,
https://doi.org/10.2298/JSC190930129K . .

TY  - JOUR
AU  - Balaž, Ana Marija
AU  - Stevanović, Jelena
AU  - Ostafe, Raluca
AU  - Blažić, Marija
AU  - Ilić Đurđić, Karla
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3128
AB  - Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from white rot fungi Phanerochaete chrysosporium can be used for constructing biosensors and biofuel cells, for bleaching cotton in textile industry, and recently, the enzyme has found an important application in biomedicine as an antimicrobial and antibiofilm agent. Stability and activity of the wild-type (wt) CDH and mutants at methionine residues in the presence of hydrogen peroxide were investigated. Saturation mutagenesis libraries were made at the only methionine in heme domain M65 and two methionines M685 and M738 in the flavin domain that were closest to the active site. After screening the libraries, three mutants with increased activity and stability in the presence of peroxide were found, M65F with 70% of residual activity after 6 h of incubation in 0.3 M hydrogen peroxide, M738S with 80% of residual activity and M685Y with over 90% of residual activity compared to wild-type CDH that retained 40% of original activity. Combined mutants showed no activity. The most stable mutant M685Y with 5.8 times increased half-life in the presence of peroxide showed also 2.5 times increased kcat for lactose compared to wtCDH and could be good candidate for applications in biofuel cells and biocatalysis for lactobionic acid production.
PB  - Springer International Publishing
T2  - Molecular Diversity
T1  - Semi‑rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide
VL  - 24
SP  - 593
EP  - 601
DO  - 10.1007/s11030-019-09965-0
ER  - 

@article{
author = "Balaž, Ana Marija and Stevanović, Jelena and Ostafe, Raluca and Blažić, Marija and Ilić Đurđić, Karla and Fischer, Rainer and Prodanović, Radivoje",
year = "2020",
abstract = "Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from white rot fungi Phanerochaete chrysosporium can be used for constructing biosensors and biofuel cells, for bleaching cotton in textile industry, and recently, the enzyme has found an important application in biomedicine as an antimicrobial and antibiofilm agent. Stability and activity of the wild-type (wt) CDH and mutants at methionine residues in the presence of hydrogen peroxide were investigated. Saturation mutagenesis libraries were made at the only methionine in heme domain M65 and two methionines M685 and M738 in the flavin domain that were closest to the active site. After screening the libraries, three mutants with increased activity and stability in the presence of peroxide were found, M65F with 70% of residual activity after 6 h of incubation in 0.3 M hydrogen peroxide, M738S with 80% of residual activity and M685Y with over 90% of residual activity compared to wild-type CDH that retained 40% of original activity. Combined mutants showed no activity. The most stable mutant M685Y with 5.8 times increased half-life in the presence of peroxide showed also 2.5 times increased kcat for lactose compared to wtCDH and could be good candidate for applications in biofuel cells and biocatalysis for lactobionic acid production.",
publisher = "Springer International Publishing",
journal = "Molecular Diversity",
title = "Semi‑rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide",
volume = "24",
pages = "593-601",
doi = "10.1007/s11030-019-09965-0"
}

Balaž, A. M., Stevanović, J., Ostafe, R., Blažić, M., Ilić Đurđić, K., Fischer, R.,& Prodanović, R.. (2020). Semi‑rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide. in Molecular Diversity
Springer International Publishing., 24, 593-601.
https://doi.org/10.1007/s11030-019-09965-0

Balaž AM, Stevanović J, Ostafe R, Blažić M, Ilić Đurđić K, Fischer R, Prodanović R. Semi‑rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide. in Molecular Diversity. 2020;24:593-601.
doi:10.1007/s11030-019-09965-0 .

Balaž, Ana Marija, Stevanović, Jelena, Ostafe, Raluca, Blažić, Marija, Ilić Đurđić, Karla, Fischer, Rainer, Prodanović, Radivoje, "Semi‑rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide" in Molecular Diversity, 24 (2020):593-601,
https://doi.org/10.1007/s11030-019-09965-0 . .

TY  - JOUR
AU  - Ilić Đurđić, Karla
AU  - Ece, Selin
AU  - Ostafe, Raluca
AU  - Vogel, Simon
AU  - Balaž, Ana Marija
AU  - Schillberg, Stefan
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3974
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3580
AB  - Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H2O2-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we generated a random mutagenesis library comprising 106 mutated LiP genes and screened for expressed enzymes with higher than wild-type activity after incubation in 30 mM H2O2 by flow cytometry with fluorescein-tyramide as a substrate. To preserve the genotype-phenotype connection, the LiP mutants were displayed on the yeast cell surface. Two rounds of sorting were performed, recovered colonies were then screened in microtiter plates, and activity analysis revealed a significant increase in the percentage of cells expressing LiP variants with higher oxidative stability than wtLiP. Two rounds of sorting increased the proportion of more-stable variants from 1.4% in the original library to 52.3%. The most stable variants after two rounds of sorting featured between two and four mutations and retained up to 80% of initial activity after 1 h incubation in 30 mM H2O2. We for the first-time applied flow cytometry for screening of any ligninolytic peroxidase library. Obtained results suggest that developed system may be applied for improvement of industrially important characteristics of lignin peroxidase.
PB  - Elsevier
T2  - Journal of Bioscience and Bioengineering
T1  - Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability
VL  - 129
IS  - 6
SP  - 664
EP  - 671
DO  - 10.1016/j.jbiosc.2019.12.009
ER  - 

@article{
author = "Ilić Đurđić, Karla and Ece, Selin and Ostafe, Raluca and Vogel, Simon and Balaž, Ana Marija and Schillberg, Stefan and Fischer, Rainer and Prodanović, Radivoje",
year = "2020",
abstract = "Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H2O2-dependent manner. Its ability to oxidize many pollutants makes it suitable for bioremediation applications and an ideal candidate for optimization by mutagenesis and selection. In order to increase oxidative stability of LiP we generated a random mutagenesis library comprising 106 mutated LiP genes and screened for expressed enzymes with higher than wild-type activity after incubation in 30 mM H2O2 by flow cytometry with fluorescein-tyramide as a substrate. To preserve the genotype-phenotype connection, the LiP mutants were displayed on the yeast cell surface. Two rounds of sorting were performed, recovered colonies were then screened in microtiter plates, and activity analysis revealed a significant increase in the percentage of cells expressing LiP variants with higher oxidative stability than wtLiP. Two rounds of sorting increased the proportion of more-stable variants from 1.4% in the original library to 52.3%. The most stable variants after two rounds of sorting featured between two and four mutations and retained up to 80% of initial activity after 1 h incubation in 30 mM H2O2. We for the first-time applied flow cytometry for screening of any ligninolytic peroxidase library. Obtained results suggest that developed system may be applied for improvement of industrially important characteristics of lignin peroxidase.",
publisher = "Elsevier",
journal = "Journal of Bioscience and Bioengineering",
title = "Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability",
volume = "129",
number = "6",
pages = "664-671",
doi = "10.1016/j.jbiosc.2019.12.009"
}

Ilić Đurđić, K., Ece, S., Ostafe, R., Vogel, S., Balaž, A. M., Schillberg, S., Fischer, R.,& Prodanović, R.. (2020). Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability. in Journal of Bioscience and Bioengineering
Elsevier., 129(6), 664-671.
https://doi.org/10.1016/j.jbiosc.2019.12.009

Ilić Đurđić K, Ece S, Ostafe R, Vogel S, Balaž AM, Schillberg S, Fischer R, Prodanović R. Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability. in Journal of Bioscience and Bioengineering. 2020;129(6):664-671.
doi:10.1016/j.jbiosc.2019.12.009 .

Ilić Đurđić, Karla, Ece, Selin, Ostafe, Raluca, Vogel, Simon, Balaž, Ana Marija, Schillberg, Stefan, Fischer, Rainer, Prodanović, Radivoje, "Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability" in Journal of Bioscience and Bioengineering, 129, no. 6 (2020):664-671,
https://doi.org/10.1016/j.jbiosc.2019.12.009 . .

TY  - JOUR
AU  - Balaž, Ana Marija
AU  - Blažić, Marija
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3880
AB  - Production of soluble cellobiose dehydrogenase (CDH) mutant proteins previously evolved on the surface of S. cerevisiae yeast cells was established for use in biosensors and biofuel cells. For this purpose, mutant cdh genes tm (D20N, A64T, V592M), H5 (D20N, V22A, A64T, V592M) and H9 (D20N, A64T, T84A, A261P, V592M, E674G, N715S) were cloned to pPICZα plasmid and transformed into Pichia pastoris KM71H strain for high expression in a soluble form and kinetic characterization. After 6 days of expression under methanol induction, the CDHs were purified by ultrafiltration, ion- -exchange chromatography and gel filtration. Sodium dodecyl sulfate electrophoresis confirmed the purity and presence of a single protein band at a molecular weight of 100 kDa. Kinetic characterization showed that the H5 mutant had the highest catalytic constant of 43.5 s-1 for lactose, while the mutant H9 showed the highest specificity constant for lactose of 132 mM-1 s-1. All three mutant proteins did not change the pH optimum that was between 4.5 and 5.5. Compared to the previously obtained wild types and mutants of CDH from Phanerochaete chrysosporium, the variants reported in this article had higher activity and specificity that together with high protein expression rate in P. pastoris, makes them good candidates for use in biotechnology for lactobionic acid production and biosensor manufacture.
AB  - У циљу употребе у биосензорима и биогоривним ћелијама, успостављена је производња
растворних облика целобиоза дехидрогеназе (CDH) претходно еволуираних на површини
квашчевих ћелија S. cerevisiae. У ту сврху су мутанти CDH, tm (D20N, A64T, V592M), H5
(D20N, V22A, A64T, V592M) и H9 (D20N, A64T, T84A, A261P, V592M, E674G, N715S)
клонирани у pPICZα плазмид и трансформисани у Pichia pastoris KM71H сој за високу
експресију у растворном облику и кинетичку карактеризацију. После 6 дана експресије под
индукцијом метанолом, мутанти су пречишћени ултрафилтрацијом, јоноизмењивачком
хроматографијом и гел-филтрацијом. SDS електрофореза је потврдила чистоћу уз присуство
једне протеинске траке молекулскe масe од 100 kDa. Кинетичка карактеризација је показала
да H5 мутирани протеин поседује највећу каталитичку константу од 43,5 s-1 за лактозу, док
је H9 имао највећу константу специфичности за лактозу од 132 mM-1 s-1. Сва три мутирана
протеина су имала неизмењен pH оптимум који је био у опсегу од 4,5 до 5,5. У поређењу са
претходно добијеним природним и мутантним облицима CDH протеина из Phanerochaete
chrysosporium, облици приказани у овом раду имају већу активност и специфичност, што их,
повезано са високом експресијом протеина у P. Pastoris, чини добрим кандидатима за упо-
требу у биотехнологији за производњу лактобионске киселине и биосензора.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain
T1  - Ekspresija, prečišćavanje i karakterizacija mutanata celobioza - dehidrogenaze iz Phanerochaete chrysosporium u Pichia pastoris KM71H soju
VL  - 85
IS  - 1
SP  - 25
EP  - 35
DO  - 10.2298/JSC190320058B
ER  - 

@article{
author = "Balaž, Ana Marija and Blažić, Marija and Popović, Nikolina and Prodanović, Olivera and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2020",
abstract = "Production of soluble cellobiose dehydrogenase (CDH) mutant proteins previously evolved on the surface of S. cerevisiae yeast cells was established for use in biosensors and biofuel cells. For this purpose, mutant cdh genes tm (D20N, A64T, V592M), H5 (D20N, V22A, A64T, V592M) and H9 (D20N, A64T, T84A, A261P, V592M, E674G, N715S) were cloned to pPICZα plasmid and transformed into Pichia pastoris KM71H strain for high expression in a soluble form and kinetic characterization. After 6 days of expression under methanol induction, the CDHs were purified by ultrafiltration, ion- -exchange chromatography and gel filtration. Sodium dodecyl sulfate electrophoresis confirmed the purity and presence of a single protein band at a molecular weight of 100 kDa. Kinetic characterization showed that the H5 mutant had the highest catalytic constant of 43.5 s-1 for lactose, while the mutant H9 showed the highest specificity constant for lactose of 132 mM-1 s-1. All three mutant proteins did not change the pH optimum that was between 4.5 and 5.5. Compared to the previously obtained wild types and mutants of CDH from Phanerochaete chrysosporium, the variants reported in this article had higher activity and specificity that together with high protein expression rate in P. pastoris, makes them good candidates for use in biotechnology for lactobionic acid production and biosensor manufacture., У циљу употребе у биосензорима и биогоривним ћелијама, успостављена је производња
растворних облика целобиоза дехидрогеназе (CDH) претходно еволуираних на површини
квашчевих ћелија S. cerevisiae. У ту сврху су мутанти CDH, tm (D20N, A64T, V592M), H5
(D20N, V22A, A64T, V592M) и H9 (D20N, A64T, T84A, A261P, V592M, E674G, N715S)
клонирани у pPICZα плазмид и трансформисани у Pichia pastoris KM71H сој за високу
експресију у растворном облику и кинетичку карактеризацију. После 6 дана експресије под
индукцијом метанолом, мутанти су пречишћени ултрафилтрацијом, јоноизмењивачком
хроматографијом и гел-филтрацијом. SDS електрофореза је потврдила чистоћу уз присуство
једне протеинске траке молекулскe масe од 100 kDa. Кинетичка карактеризација је показала
да H5 мутирани протеин поседује највећу каталитичку константу од 43,5 s-1 за лактозу, док
је H9 имао највећу константу специфичности за лактозу од 132 mM-1 s-1. Сва три мутирана
протеина су имала неизмењен pH оптимум који је био у опсегу од 4,5 до 5,5. У поређењу са
претходно добијеним природним и мутантним облицима CDH протеина из Phanerochaete
chrysosporium, облици приказани у овом раду имају већу активност и специфичност, што их,
повезано са високом експресијом протеина у P. Pastoris, чини добрим кандидатима за упо-
требу у биотехнологији за производњу лактобионске киселине и биосензора.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain, Ekspresija, prečišćavanje i karakterizacija mutanata celobioza - dehidrogenaze iz Phanerochaete chrysosporium u Pichia pastoris KM71H soju",
volume = "85",
number = "1",
pages = "25-35",
doi = "10.2298/JSC190320058B"
}

Balaž, A. M., Blažić, M., Popović, N., Prodanović, O., Ostafe, R., Fischer, R.,& Prodanović, R.. (2020). Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 85(1), 25-35.
https://doi.org/10.2298/JSC190320058B

Balaž AM, Blažić M, Popović N, Prodanović O, Ostafe R, Fischer R, Prodanović R. Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain. in Journal of the Serbian Chemical Society. 2020;85(1):25-35.
doi:10.2298/JSC190320058B .

Balaž, Ana Marija, Blažić, Marija, Popović, Nikolina, Prodanović, Olivera, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain" in Journal of the Serbian Chemical Society, 85, no. 1 (2020):25-35,
https://doi.org/10.2298/JSC190320058B . .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Spasojević, Pavle
AU  - Šešlija, Sanja
AU  - Popović, Ivanka G.
AU  - Veljović, Đorđe
AU  - Pjanović, Rada
AU  - Panić, Vesna
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2990
AB  - The design of complex polymeric materials with predefined task-specific properties meets challenging issues: to establish synthesis-structure-properties relationship, as well as to understand the arrangement and interactions of the involved components. Hybrid networks of protein (casein) and polyelectrolyte (poly(methacrylic acid)) were synthesized and their swelling, dynamic-mechanical and morphological properties were investigated as functions of the neutralization degree of methacrylic acid and the concentrations of caseinate and crosslinker. The domination of different interactions between components led to diverse forms of casein macromolecules- from micelles to unfold chains. That resulted in very different structures and thereby properties of derived hybrid networks: from porous unswellable to highly swellable and reinforced ones. A great opportunity to easily modulate the final characteristics of the reported hybrid system to fit the need of specific application just through the change of one synthesis parameter was thereby demonstrated.
PB  - Elsevier
T2  - European Polymer Journal
T1  - Casein-poly(methacrylic acid) hybrid soft networks with easy tunable properties
VL  - 113
SP  - 276
EP  - 288
DO  - 10.1016/j.eurpolymj.2019.01.065
ER  - 

@article{
author = "Marković, Maja D. and Spasojević, Pavle and Šešlija, Sanja and Popović, Ivanka G. and Veljović, Đorđe and Pjanović, Rada and Panić, Vesna",
year = "2019",
abstract = "The design of complex polymeric materials with predefined task-specific properties meets challenging issues: to establish synthesis-structure-properties relationship, as well as to understand the arrangement and interactions of the involved components. Hybrid networks of protein (casein) and polyelectrolyte (poly(methacrylic acid)) were synthesized and their swelling, dynamic-mechanical and morphological properties were investigated as functions of the neutralization degree of methacrylic acid and the concentrations of caseinate and crosslinker. The domination of different interactions between components led to diverse forms of casein macromolecules- from micelles to unfold chains. That resulted in very different structures and thereby properties of derived hybrid networks: from porous unswellable to highly swellable and reinforced ones. A great opportunity to easily modulate the final characteristics of the reported hybrid system to fit the need of specific application just through the change of one synthesis parameter was thereby demonstrated.",
publisher = "Elsevier",
journal = "European Polymer Journal",
title = "Casein-poly(methacrylic acid) hybrid soft networks with easy tunable properties",
volume = "113",
pages = "276-288",
doi = "10.1016/j.eurpolymj.2019.01.065"
}

Marković, M. D., Spasojević, P., Šešlija, S., Popović, I. G., Veljović, Đ., Pjanović, R.,& Panić, V.. (2019). Casein-poly(methacrylic acid) hybrid soft networks with easy tunable properties. in European Polymer Journal
Elsevier., 113, 276-288.
https://doi.org/10.1016/j.eurpolymj.2019.01.065

Marković MD, Spasojević P, Šešlija S, Popović IG, Veljović Đ, Pjanović R, Panić V. Casein-poly(methacrylic acid) hybrid soft networks with easy tunable properties. in European Polymer Journal. 2019;113:276-288.
doi:10.1016/j.eurpolymj.2019.01.065 .

Marković, Maja D., Spasojević, Pavle, Šešlija, Sanja, Popović, Ivanka G., Veljović, Đorđe, Pjanović, Rada, Panić, Vesna, "Casein-poly(methacrylic acid) hybrid soft networks with easy tunable properties" in European Polymer Journal, 113 (2019):276-288,
https://doi.org/10.1016/j.eurpolymj.2019.01.065 . .

TY  - THES
AU  - Balaž, Ana Marija
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7704
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22916/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=23928841
UR  - https://nardus.mpn.gov.rs/handle/123456789/17616
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3738
AB  - Celobioza – dehidrogenaza poreklom iz Phanerochaete chrysosporium, gljive bele truleži, pripada ekstracelularnim oksidoredukcionim enzimima i katalizuje oksidaciju β – 1,4 – glikozidno vezanih oligosaharida poput celobioze i laktoze. Oksidacijom laktoze dolazi do formiranja laktobionske kiseline koja pronalazi veliku primenu u farmaceutskoj i kozmetičkoj industriji gde se koristi prilikom distribucije lekova i za hidrataciju kože kao sastavni deo različitih krema, gde zajedno sa hijaluronskom kiselinu ima ulogu u smanjenju bora.Celobioza – dehidrogenaza prilikom oksidacije laktoze ili celobioze, kao prirodnih supstrata, katalizuje redukciju jednog dvoelektronskog ili dva jednoelektronska akceptora elektrona. Jedan od najkorišćenijih dvoelektronskih akceptora elektrona je upravo dihlor fenol indofenol (DCIP), dok jednoelektronski akceptori elektrona mogu biti citohrom c, ABTS, ali i Fe3+ i Mn3+ joni. Redukcijom Fe3+ jona u prisustvu molekulskog kiseonika dolazi do formiranja vodonik peroksida i posredstvom Fentonove reakcije do generisanja hidroksil radikala.Polazeći od ove činjenice, iskoristili smo upravo Fentonovu reakciju za razvoj fluorescentnog eseja za visoko efikasnu pretragu biblioteka gena celobioza – dehidrogenaze, baziranog na detekciji proizvedenih hidroksil radikala fluorogenom probom aminofenil – fluoresceinom (APF).Primena celobioza – dehidrogenaze u konstruisanju biosenzora i biogorivnih ćelija leži upravo u njenoj sposobnosti da katalizuje oksidaciju laktoze, celobioze i β – 1,4 – vezanih oligosaharida do odgovarajućih laktona koji potom spontano hidrolizuju do aldonskih kiselina. Enzimi koji nalaze primenu u konstruisanju biosenzora i biogorivnih ćelija, moraju da zadovoljavaju nekoliko kriterijuma,odnosno moraju da imaju veliku osetljivost i supstratnu specifičnost, ali i da pokazuju povećanu stabilnost...
AB  - Cellobiose dehydrogenase from Phanerochaete chrysosporium, a white rot fungus, belongs to the extracellular oxidoreductive group of enzymes and catalyzes the oxidation of the β – 1,4 – glycoside bond of oligosaccharides such as cellobiose and lactose. During oxidation of lactose, formation of lactobionic acid occurs which has many applications in pharmaceutical and cosmetic industry. Such applications include the distribution of medicine, a component responsible for skin hydration and when combined with hyaluronic acid as an agent against wrinkles.During oxidation of lactose or cellobiose by cellobiose dehydrogenase, reduction catalysis occurs of one two electron or two one electron acceptors. One of the most utilized two electron acceptors is DCIP, while one electron acceptors are usually cytochrome c, ABTS, Fe3+ and Mn3+ ions. During reduction of Fe3+ ions in the presence of molecular oxygen, H2O2 is formed and due to the Fenton reaction formation of hydroxyl radicals occurs. Due to this occurrence we wanted to use the Fenton reaction in order to develop a fluorescent assay based on the production of hydroxyl radicals and the fluorescence of aminophenyl fluorescein (APF). This would allow us to efficiently analyze cellobiose dehydrogenase gene libraries.With this fact in mind, the Fenton reaction was used to develop a fluorescent assay for the high throughput screening of cellobiose dehydrogenase genes, based on the detection of hydroxyl radicals with the fluorescent probe APF.The possible application of cellobiose dehydrogenase in the construction of various biosensors and biofuel cells is due the its ability to catalyze the oxidation of lactose, cellobiose and similar β – 1,4 – oligosaccharides do their corresponding lactones which then spontaneously hydrolyze to aldonic acids. Enzymes used in suchapplications need to satisfy certain criteria, such as exceptional sensitivity, substrate selectivity, stability and activity...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Proteinski inženjering celobioza - dehidrogenaze iz Phanerochaete chrysosporium u cilju povećanja oksidativne stabilnosti za primenu u biokatalizi
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17616
ER  - 

@phdthesis{
author = "Balaž, Ana Marija",
year = "2019",
abstract = "Celobioza – dehidrogenaza poreklom iz Phanerochaete chrysosporium, gljive bele truleži, pripada ekstracelularnim oksidoredukcionim enzimima i katalizuje oksidaciju β – 1,4 – glikozidno vezanih oligosaharida poput celobioze i laktoze. Oksidacijom laktoze dolazi do formiranja laktobionske kiseline koja pronalazi veliku primenu u farmaceutskoj i kozmetičkoj industriji gde se koristi prilikom distribucije lekova i za hidrataciju kože kao sastavni deo različitih krema, gde zajedno sa hijaluronskom kiselinu ima ulogu u smanjenju bora.Celobioza – dehidrogenaza prilikom oksidacije laktoze ili celobioze, kao prirodnih supstrata, katalizuje redukciju jednog dvoelektronskog ili dva jednoelektronska akceptora elektrona. Jedan od najkorišćenijih dvoelektronskih akceptora elektrona je upravo dihlor fenol indofenol (DCIP), dok jednoelektronski akceptori elektrona mogu biti citohrom c, ABTS, ali i Fe3+ i Mn3+ joni. Redukcijom Fe3+ jona u prisustvu molekulskog kiseonika dolazi do formiranja vodonik peroksida i posredstvom Fentonove reakcije do generisanja hidroksil radikala.Polazeći od ove činjenice, iskoristili smo upravo Fentonovu reakciju za razvoj fluorescentnog eseja za visoko efikasnu pretragu biblioteka gena celobioza – dehidrogenaze, baziranog na detekciji proizvedenih hidroksil radikala fluorogenom probom aminofenil – fluoresceinom (APF).Primena celobioza – dehidrogenaze u konstruisanju biosenzora i biogorivnih ćelija leži upravo u njenoj sposobnosti da katalizuje oksidaciju laktoze, celobioze i β – 1,4 – vezanih oligosaharida do odgovarajućih laktona koji potom spontano hidrolizuju do aldonskih kiselina. Enzimi koji nalaze primenu u konstruisanju biosenzora i biogorivnih ćelija, moraju da zadovoljavaju nekoliko kriterijuma,odnosno moraju da imaju veliku osetljivost i supstratnu specifičnost, ali i da pokazuju povećanu stabilnost..., Cellobiose dehydrogenase from Phanerochaete chrysosporium, a white rot fungus, belongs to the extracellular oxidoreductive group of enzymes and catalyzes the oxidation of the β – 1,4 – glycoside bond of oligosaccharides such as cellobiose and lactose. During oxidation of lactose, formation of lactobionic acid occurs which has many applications in pharmaceutical and cosmetic industry. Such applications include the distribution of medicine, a component responsible for skin hydration and when combined with hyaluronic acid as an agent against wrinkles.During oxidation of lactose or cellobiose by cellobiose dehydrogenase, reduction catalysis occurs of one two electron or two one electron acceptors. One of the most utilized two electron acceptors is DCIP, while one electron acceptors are usually cytochrome c, ABTS, Fe3+ and Mn3+ ions. During reduction of Fe3+ ions in the presence of molecular oxygen, H2O2 is formed and due to the Fenton reaction formation of hydroxyl radicals occurs. Due to this occurrence we wanted to use the Fenton reaction in order to develop a fluorescent assay based on the production of hydroxyl radicals and the fluorescence of aminophenyl fluorescein (APF). This would allow us to efficiently analyze cellobiose dehydrogenase gene libraries.With this fact in mind, the Fenton reaction was used to develop a fluorescent assay for the high throughput screening of cellobiose dehydrogenase genes, based on the detection of hydroxyl radicals with the fluorescent probe APF.The possible application of cellobiose dehydrogenase in the construction of various biosensors and biofuel cells is due the its ability to catalyze the oxidation of lactose, cellobiose and similar β – 1,4 – oligosaccharides do their corresponding lactones which then spontaneously hydrolyze to aldonic acids. Enzymes used in suchapplications need to satisfy certain criteria, such as exceptional sensitivity, substrate selectivity, stability and activity...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Proteinski inženjering celobioza - dehidrogenaze iz Phanerochaete chrysosporium u cilju povećanja oksidativne stabilnosti za primenu u biokatalizi",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17616"
}

Balaž, A. M.. (2019). Proteinski inženjering celobioza - dehidrogenaze iz Phanerochaete chrysosporium u cilju povećanja oksidativne stabilnosti za primenu u biokatalizi. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_17616

Balaž AM. Proteinski inženjering celobioza - dehidrogenaze iz Phanerochaete chrysosporium u cilju povećanja oksidativne stabilnosti za primenu u biokatalizi. in Универзитет у Београду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_17616 .

Balaž, Ana Marija, "Proteinski inženjering celobioza - dehidrogenaze iz Phanerochaete chrysosporium u cilju povećanja oksidativne stabilnosti za primenu u biokatalizi" in Универзитет у Београду (2019),
https://hdl.handle.net/21.15107/rcub_nardus_17616 .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Wan Abd Al Qadr Imad, Wan-Mohtar
AU  - Nikšić, Miomir
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2992
AB  - Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin’s barrier function, and provide a skinlightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant
components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction
(HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50=1.65±0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes
VL  - 71
IS  - 2
SP  - 253
EP  - 264
DO  - 10.2298/ABS181217007K
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Wan Abd Al Qadr Imad, Wan-Mohtar and Nikšić, Miomir",
year = "2019",
abstract = "Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin’s barrier function, and provide a skinlightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant
components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction
(HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50=1.65±0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes",
volume = "71",
number = "2",
pages = "253-264",
doi = "10.2298/ABS181217007K"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Wan Abd Al Qadr Imad, W.,& Nikšić, M.. (2019). Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences
Serbian Biological Society., 71(2), 253-264.
https://doi.org/10.2298/ABS181217007K

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Wan Abd Al Qadr Imad W, Nikšić M. Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes. in Archives of Biological Sciences. 2019;71(2):253-264.
doi:10.2298/ABS181217007K .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Wan Abd Al Qadr Imad, Wan-Mohtar, Nikšić, Miomir, "Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes" in Archives of Biological Sciences, 71, no. 2 (2019):253-264,
https://doi.org/10.2298/ABS181217007K . .

TY  - THES
AU  - Zarić, Milana
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6239
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18846/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50656271
UR  - http://nardus.mpn.gov.rs/123456789/10225
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2592
AB  - Poznavanje termodinamičkih svojstava tečnih supstanci i njihovih smešaznačajno je za optimizaciju industrijskih procesa i za projektovanje industrijskihpostrojenja. Cilj ove doktorske disertacije je upoređivanje svojstava zasićenih inezasićenih organskih jedinjenja, njihovih termodinamičkih karakteristika,međumolekulskih interakcija i ponašanja u smešama. Ispitivani su zasićeni i nezasićenialkoholi i njihove smeše sa alkanima, jer ova jedinjenja su veoma značajna u industriji,naročito u naftnoj industriji, industriji polimera i prehrambenoj industriji.Eksperimentalno su izmerena termodinamička svojstava zasićenih i nezasićenihalkohola i njihovih smeša sa alkanima i na osnovu izmerenih vrednosti određene sudopunske veličine pri mešanju tečnosti. Urađena je FT-IR analiza ovih sistema naosnovu koje su dobijeni podaci o intermolekulskim interakcijama, a kvantnomehaničkim metodama su modelovane interakcije u proučavanim čistim tečnostima injihovim smešama.Podaci dobijeni na osnovu eksperimentalnih merenja pokazuju mnoge sličnostismeša nezasićenih i zasićenih alkohola, ali ukazuju i na značajne razlike. Najznačajnijarazlika između dva proučavana sistema je uočena u vrednostima promene viskoznosti.Promena viskoznosti je značajno veća za smešu zasićenih jedinjenja, dok manjapromena viskoznosti u smeši nezasićenog alkohola i alkana ukazuje na veće trenje, štose može objasniti interakcijama dvostruke veze ili možda i specifičnom konformacijomovog alkohola prouzrokovanom prisustvom dvostruke veze. Poređenje FT-IR spektaračistog nezasićenog alkohola i smeše sa alkanom ukazuju na promenu u interakcijamadvostruke veze alkohola.Rezultati veoma pouzdanog kvantno mehaničkog modelovanja interakcija umodel sistemima sa nezasićenim i zasićenim molekulima pokazuju da su interakcijeizmeđu molekula sa jednostrukom i dvostrukom vezom jače od interakcija između dvamolekula sa jednostrukim vezama i dva molekula sa dvostrukim vezama. Ovi rezultatisu u skladu sa eksperimentalno opaženim većim trenjem u smeši nezasićenog alkohola ialkana. Modelovanje FT-IR spektara pokazuje da interakcija između dvostruke veze ialkoholne –OH grupe utiče na ponašanje nezasićenog alkohola u smešama.Eksperimentalni rezultati u ovoj doktorskoj disertaciji ukazuju na razlike uponašanju smeša zasićenih i nezasićenih alkohola, dok rezultati modelovanja interakcijamogu da objasne uticaj dvostrukih veza na ponašanje smeša.
AB  - Data on the thermodynamic properties of liquid substances and their mixtures isimportant for optimizing industrial processes and design of industrial plants. The goal ofthis doctoral dissertation is to compare the properties of saturated and unsaturatedorganic compounds, their thermodynamic characteristics, intermolecular interactionsand behavior in mixtures. Saturated and unsaturated alcohols and their mixtures withalkanes were studied, since these compounds are very important in the industry,especially in the petrochemical industry, the polymer industry and the food industry.The thermodynamic properties of saturated and unsaturated alcohols and theirmixtures with alkanes were measured experimentally and, the results of measuredvalues were further used to determine excess and deviation properties in the mixtures.Based on the FT-IR analysis of these systems data on intermolecular interactions wereobtained, while using quantum mechanical methods interactions of pure liquids andtheir mixtures were modeled.The data obtained from experimental measurements indicate significantsimilarities of mixtures of unsaturated and saturated alcohols, however, also indicatesome important differences. The most significant difference between the two studiedsystems is observed in the values of viscosity deviation. The viscosity deviation issignificantly higher for the mixture of saturated compounds, while a slight change inviscosity of the mixture of unsaturated alcohol and alkane indicates higher friction. Thiscan be explained by interactions of the double bond or perhaps by the specificconformation of the alcohol caused by the presence of a double bond. Comparison ofthe FT-IR spectra of pure unsaturated alcohol and mixture with alkane indicates achange in the interaction of the double bond of alcohol.The results of a very reliable quantum mechanical modeling of interactions inmodel systems with unsaturated and saturated molecules show that the interactionsbetween molecules with single and double bonds are stronger than the interactionsbetween two molecules with single bond, and two molecules with double bonds. Theseresults are in accordance with experimentally observed higher friction in the mixture ofunsaturated alcohol and alkane. Modeling the FT-IR spectra indicates the effect of theinteraction between the double bond and the alcohol-OH group on the behavior ofunsaturated alcohols in the mixtures.Experimental results in this doctoral dissertation can point out the differences inthe behavior of mixtures of saturated and unsaturated alcohols, while the results ofinteraction modeling can explain the effect of double bond on behavior of mixtures.
PB  - Универзитет у Београду, Технолошко-металуршки факултет
T2  - Универзитет у Београду
T1  - Eksperimentalno određivanje volumetrijskih i stukturnih svojstava i modelovanje smeša nezasićenih organskih jedinjenja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10225
ER  - 

@phdthesis{
author = "Zarić, Milana",
year = "2018",
abstract = "Poznavanje termodinamičkih svojstava tečnih supstanci i njihovih smešaznačajno je za optimizaciju industrijskih procesa i za projektovanje industrijskihpostrojenja. Cilj ove doktorske disertacije je upoređivanje svojstava zasićenih inezasićenih organskih jedinjenja, njihovih termodinamičkih karakteristika,međumolekulskih interakcija i ponašanja u smešama. Ispitivani su zasićeni i nezasićenialkoholi i njihove smeše sa alkanima, jer ova jedinjenja su veoma značajna u industriji,naročito u naftnoj industriji, industriji polimera i prehrambenoj industriji.Eksperimentalno su izmerena termodinamička svojstava zasićenih i nezasićenihalkohola i njihovih smeša sa alkanima i na osnovu izmerenih vrednosti određene sudopunske veličine pri mešanju tečnosti. Urađena je FT-IR analiza ovih sistema naosnovu koje su dobijeni podaci o intermolekulskim interakcijama, a kvantnomehaničkim metodama su modelovane interakcije u proučavanim čistim tečnostima injihovim smešama.Podaci dobijeni na osnovu eksperimentalnih merenja pokazuju mnoge sličnostismeša nezasićenih i zasićenih alkohola, ali ukazuju i na značajne razlike. Najznačajnijarazlika između dva proučavana sistema je uočena u vrednostima promene viskoznosti.Promena viskoznosti je značajno veća za smešu zasićenih jedinjenja, dok manjapromena viskoznosti u smeši nezasićenog alkohola i alkana ukazuje na veće trenje, štose može objasniti interakcijama dvostruke veze ili možda i specifičnom konformacijomovog alkohola prouzrokovanom prisustvom dvostruke veze. Poređenje FT-IR spektaračistog nezasićenog alkohola i smeše sa alkanom ukazuju na promenu u interakcijamadvostruke veze alkohola.Rezultati veoma pouzdanog kvantno mehaničkog modelovanja interakcija umodel sistemima sa nezasićenim i zasićenim molekulima pokazuju da su interakcijeizmeđu molekula sa jednostrukom i dvostrukom vezom jače od interakcija između dvamolekula sa jednostrukim vezama i dva molekula sa dvostrukim vezama. Ovi rezultatisu u skladu sa eksperimentalno opaženim većim trenjem u smeši nezasićenog alkohola ialkana. Modelovanje FT-IR spektara pokazuje da interakcija između dvostruke veze ialkoholne –OH grupe utiče na ponašanje nezasićenog alkohola u smešama.Eksperimentalni rezultati u ovoj doktorskoj disertaciji ukazuju na razlike uponašanju smeša zasićenih i nezasićenih alkohola, dok rezultati modelovanja interakcijamogu da objasne uticaj dvostrukih veza na ponašanje smeša., Data on the thermodynamic properties of liquid substances and their mixtures isimportant for optimizing industrial processes and design of industrial plants. The goal ofthis doctoral dissertation is to compare the properties of saturated and unsaturatedorganic compounds, their thermodynamic characteristics, intermolecular interactionsand behavior in mixtures. Saturated and unsaturated alcohols and their mixtures withalkanes were studied, since these compounds are very important in the industry,especially in the petrochemical industry, the polymer industry and the food industry.The thermodynamic properties of saturated and unsaturated alcohols and theirmixtures with alkanes were measured experimentally and, the results of measuredvalues were further used to determine excess and deviation properties in the mixtures.Based on the FT-IR analysis of these systems data on intermolecular interactions wereobtained, while using quantum mechanical methods interactions of pure liquids andtheir mixtures were modeled.The data obtained from experimental measurements indicate significantsimilarities of mixtures of unsaturated and saturated alcohols, however, also indicatesome important differences. The most significant difference between the two studiedsystems is observed in the values of viscosity deviation. The viscosity deviation issignificantly higher for the mixture of saturated compounds, while a slight change inviscosity of the mixture of unsaturated alcohol and alkane indicates higher friction. Thiscan be explained by interactions of the double bond or perhaps by the specificconformation of the alcohol caused by the presence of a double bond. Comparison ofthe FT-IR spectra of pure unsaturated alcohol and mixture with alkane indicates achange in the interaction of the double bond of alcohol.The results of a very reliable quantum mechanical modeling of interactions inmodel systems with unsaturated and saturated molecules show that the interactionsbetween molecules with single and double bonds are stronger than the interactionsbetween two molecules with single bond, and two molecules with double bonds. Theseresults are in accordance with experimentally observed higher friction in the mixture ofunsaturated alcohol and alkane. Modeling the FT-IR spectra indicates the effect of theinteraction between the double bond and the alcohol-OH group on the behavior ofunsaturated alcohols in the mixtures.Experimental results in this doctoral dissertation can point out the differences inthe behavior of mixtures of saturated and unsaturated alcohols, while the results ofinteraction modeling can explain the effect of double bond on behavior of mixtures.",
publisher = "Универзитет у Београду, Технолошко-металуршки факултет",
journal = "Универзитет у Београду",
title = "Eksperimentalno određivanje volumetrijskih i stukturnih svojstava i modelovanje smeša nezasićenih organskih jedinjenja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10225"
}

Zarić, M.. (2018). Eksperimentalno određivanje volumetrijskih i stukturnih svojstava i modelovanje smeša nezasićenih organskih jedinjenja. in Универзитет у Београду
Универзитет у Београду, Технолошко-металуршки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_10225

Zarić M. Eksperimentalno određivanje volumetrijskih i stukturnih svojstava i modelovanje smeša nezasićenih organskih jedinjenja. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10225 .

Zarić, Milana, "Eksperimentalno određivanje volumetrijskih i stukturnih svojstava i modelovanje smeša nezasićenih organskih jedinjenja" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10225 .

TY  - JOUR
AU  - Zarić, Milana
AU  - Bugarski, Branko
AU  - Kijevčanin, Mirjana
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2155
AB  - Benchmarking study on eighteen methods, including MP2, B2PLYP-D3, B2PLYP-D3BJ, coB97xD, M05-D3, M06-D3, M052X-D3, M061-1F-D3, PBEO-D3, PBEO-D3BJ, B3LYP-D3, B3LYP-D3DJ, TPSS-D3, TPSS-D3BJ, BP86-D3, BP86-D3BJ, BLYP-D3, BLYP-D3BJ and ten basis sets: cc-pVDZ, cc-pVTZ, aug-cc-pVDZ, cc-pVQZ, def2-SVP, def2-TZVP, def2-TZVPP, def2-QZVP, 6-311++G" and 6-31G", for each method, have been performed, calculating interaction energies in (1) unsaturated/unsaturated systems (2-butene dimers), (2) unsaturated/saturated system (between butane and 2-butene) and (3) saturated/saturated (butane dimers). The calculated interaction energies are compared with accurate CCSD(T)/CBS energies. The data show that most levels of theory have the highest errors for systems with butane dimers, and calculated interaction energies in these systems are overestimated. The best levels, overall for all systems, are BLYP-D3BJ/clef2-QZVP and BLYP-D3BJ/cc-pVQZ with similar root mean square deviation (RMSD) values of 0.056 kcal mo1-1 and 0.060 kcalmorl compared to CCSD(T) values. The best level for (1) 2-butene dimers is B3LYP-D3BJ/aug-cc-pVDZ; for (2) interactions between 2-butene and butane is BLYP-D3BJ/def2-SVP; while for (3) butane dimers is BLYP-D3BJ/def2-QZVP. The differences in calculated energies among several methods are not high, however, it is important that most of the DFT methods overestimate interactions in butane dimers.
PB  - Elsevier
T2  - Computational and Theoretical Chemistry
T1  - Best methods for calculating interaction energies in 2-butene and butane systems
VL  - 1117
SP  - 150
EP  - 161
DO  - 10.1016/j.comptc.2017.08.001
ER  - 

@article{
author = "Zarić, Milana and Bugarski, Branko and Kijevčanin, Mirjana",
year = "2017",
abstract = "Benchmarking study on eighteen methods, including MP2, B2PLYP-D3, B2PLYP-D3BJ, coB97xD, M05-D3, M06-D3, M052X-D3, M061-1F-D3, PBEO-D3, PBEO-D3BJ, B3LYP-D3, B3LYP-D3DJ, TPSS-D3, TPSS-D3BJ, BP86-D3, BP86-D3BJ, BLYP-D3, BLYP-D3BJ and ten basis sets: cc-pVDZ, cc-pVTZ, aug-cc-pVDZ, cc-pVQZ, def2-SVP, def2-TZVP, def2-TZVPP, def2-QZVP, 6-311++G" and 6-31G", for each method, have been performed, calculating interaction energies in (1) unsaturated/unsaturated systems (2-butene dimers), (2) unsaturated/saturated system (between butane and 2-butene) and (3) saturated/saturated (butane dimers). The calculated interaction energies are compared with accurate CCSD(T)/CBS energies. The data show that most levels of theory have the highest errors for systems with butane dimers, and calculated interaction energies in these systems are overestimated. The best levels, overall for all systems, are BLYP-D3BJ/clef2-QZVP and BLYP-D3BJ/cc-pVQZ with similar root mean square deviation (RMSD) values of 0.056 kcal mo1-1 and 0.060 kcalmorl compared to CCSD(T) values. The best level for (1) 2-butene dimers is B3LYP-D3BJ/aug-cc-pVDZ; for (2) interactions between 2-butene and butane is BLYP-D3BJ/def2-SVP; while for (3) butane dimers is BLYP-D3BJ/def2-QZVP. The differences in calculated energies among several methods are not high, however, it is important that most of the DFT methods overestimate interactions in butane dimers.",
publisher = "Elsevier",
journal = "Computational and Theoretical Chemistry",
title = "Best methods for calculating interaction energies in 2-butene and butane systems",
volume = "1117",
pages = "150-161",
doi = "10.1016/j.comptc.2017.08.001"
}

Zarić, M., Bugarski, B.,& Kijevčanin, M.. (2017). Best methods for calculating interaction energies in 2-butene and butane systems. in Computational and Theoretical Chemistry
Elsevier., 1117, 150-161.
https://doi.org/10.1016/j.comptc.2017.08.001

Zarić M, Bugarski B, Kijevčanin M. Best methods for calculating interaction energies in 2-butene and butane systems. in Computational and Theoretical Chemistry. 2017;1117:150-161.
doi:10.1016/j.comptc.2017.08.001 .

Zarić, Milana, Bugarski, Branko, Kijevčanin, Mirjana, "Best methods for calculating interaction energies in 2-butene and butane systems" in Computational and Theoretical Chemistry, 1117 (2017):150-161,
https://doi.org/10.1016/j.comptc.2017.08.001 . .

TY  - JOUR
AU  - Jovanovic, Aleksandra A.
AU  - Dordevic, Verica B.
AU  - Zdunić, Gordana
AU  - Pljevljakusic, Dejan
AU  - Savikin, Katarina P.
AU  - Gođevac, Dejan
AU  - Bugarski, Branko
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2217
AB  - Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.
PB  - Elsevier
T2  - Separation and Purification Technology
T1  - Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques
VL  - 179
SP  - 369
EP  - 380
DO  - 10.1016/j.seppur.2017.01.055
ER  - 

@article{
author = "Jovanovic, Aleksandra A. and Dordevic, Verica B. and Zdunić, Gordana and Pljevljakusic, Dejan and Savikin, Katarina P. and Gođevac, Dejan and Bugarski, Branko",
year = "2017",
abstract = "Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.",
publisher = "Elsevier",
journal = "Separation and Purification Technology",
title = "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques",
volume = "179",
pages = "369-380",
doi = "10.1016/j.seppur.2017.01.055"
}

Jovanovic, A. A., Dordevic, V. B., Zdunić, G., Pljevljakusic, D., Savikin, K. P., Gođevac, D.,& Bugarski, B.. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology
Elsevier., 179, 369-380.
https://doi.org/10.1016/j.seppur.2017.01.055

Jovanovic AA, Dordevic VB, Zdunić G, Pljevljakusic D, Savikin KP, Gođevac D, Bugarski B. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology. 2017;179:369-380.
doi:10.1016/j.seppur.2017.01.055 .

Jovanovic, Aleksandra A., Dordevic, Verica B., Zdunić, Gordana, Pljevljakusic, Dejan, Savikin, Katarina P., Gođevac, Dejan, Bugarski, Branko, "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques" in Separation and Purification Technology, 179 (2017):369-380,
https://doi.org/10.1016/j.seppur.2017.01.055 . .

TY  - JOUR
AU  - Jovanovic, Aleksandra A.
AU  - Dordevic, Verica B.
AU  - Zdunić, Gordana
AU  - Pljevljakusic, Dejan
AU  - Savikin, Katarina P.
AU  - Gođevac, Dejan
AU  - Bugarski, Branko
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3005
AB  - Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.
PB  - Elsevier
T2  - Separation and Purification Technology
T1  - Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques
VL  - 179
SP  - 369
EP  - 380
DO  - 10.1016/j.seppur.2017.01.055
ER  - 

@article{
author = "Jovanovic, Aleksandra A. and Dordevic, Verica B. and Zdunić, Gordana and Pljevljakusic, Dejan and Savikin, Katarina P. and Gođevac, Dejan and Bugarski, Branko",
year = "2017",
abstract = "Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.",
publisher = "Elsevier",
journal = "Separation and Purification Technology",
title = "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques",
volume = "179",
pages = "369-380",
doi = "10.1016/j.seppur.2017.01.055"
}

Jovanovic, A. A., Dordevic, V. B., Zdunić, G., Pljevljakusic, D., Savikin, K. P., Gođevac, D.,& Bugarski, B.. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology
Elsevier., 179, 369-380.
https://doi.org/10.1016/j.seppur.2017.01.055

Jovanovic AA, Dordevic VB, Zdunić G, Pljevljakusic D, Savikin KP, Gođevac D, Bugarski B. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology. 2017;179:369-380.
doi:10.1016/j.seppur.2017.01.055 .

Jovanovic, Aleksandra A., Dordevic, Verica B., Zdunić, Gordana, Pljevljakusic, Dejan, Savikin, Katarina P., Gođevac, Dejan, Bugarski, Branko, "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques" in Separation and Purification Technology, 179 (2017):369-380,
https://doi.org/10.1016/j.seppur.2017.01.055 . .

TY  - JOUR
AU  - Zarić, Milana
AU  - Bugarski, Branko
AU  - Kijevčanin, Mirjana
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3034
AB  - Benchmarking study on eighteen methods, including MP2, B2PLYP-D3, B2PLYP-D3BJ, coB97xD, M05-D3, M06-D3, M052X-D3, M061-1F-D3, PBEO-D3, PBEO-D3BJ, B3LYP-D3, B3LYP-D3DJ, TPSS-D3, TPSS-D3BJ, BP86-D3, BP86-D3BJ, BLYP-D3, BLYP-D3BJ and ten basis sets: cc-pVDZ, cc-pVTZ, aug-cc-pVDZ, cc-pVQZ, def2-SVP, def2-TZVP, def2-TZVPP, def2-QZVP, 6-311++G" and 6-31G", for each method, have been performed, calculating interaction energies in (1) unsaturated/unsaturated systems (2-butene dimers), (2) unsaturated/saturated system (between butane and 2-butene) and (3) saturated/saturated (butane dimers). The calculated interaction energies are compared with accurate CCSD(T)/CBS energies. The data show that most levels of theory have the highest errors for systems with butane dimers, and calculated interaction energies in these systems are overestimated. The best levels, overall for all systems, are BLYP-D3BJ/clef2-QZVP and BLYP-D3BJ/cc-pVQZ with similar root mean square deviation (RMSD) values of 0.056 kcal mo1-1 and 0.060 kcalmorl compared to CCSD(T) values. The best level for (1) 2-butene dimers is B3LYP-D3BJ/aug-cc-pVDZ; for (2) interactions between 2-butene and butane is BLYP-D3BJ/def2-SVP; while for (3) butane dimers is BLYP-D3BJ/def2-QZVP. The differences in calculated energies among several methods are not high, however, it is important that most of the DFT methods overestimate interactions in butane dimers.
PB  - Elsevier
T2  - Computational and Theoretical Chemistry
T1  - Best methods for calculating interaction energies in 2-butene and butane systems
VL  - 1117
SP  - 150
EP  - 161
DO  - 10.1016/j.comptc.2017.08.001
ER  - 

@article{
author = "Zarić, Milana and Bugarski, Branko and Kijevčanin, Mirjana",
year = "2017",
abstract = "Benchmarking study on eighteen methods, including MP2, B2PLYP-D3, B2PLYP-D3BJ, coB97xD, M05-D3, M06-D3, M052X-D3, M061-1F-D3, PBEO-D3, PBEO-D3BJ, B3LYP-D3, B3LYP-D3DJ, TPSS-D3, TPSS-D3BJ, BP86-D3, BP86-D3BJ, BLYP-D3, BLYP-D3BJ and ten basis sets: cc-pVDZ, cc-pVTZ, aug-cc-pVDZ, cc-pVQZ, def2-SVP, def2-TZVP, def2-TZVPP, def2-QZVP, 6-311++G" and 6-31G", for each method, have been performed, calculating interaction energies in (1) unsaturated/unsaturated systems (2-butene dimers), (2) unsaturated/saturated system (between butane and 2-butene) and (3) saturated/saturated (butane dimers). The calculated interaction energies are compared with accurate CCSD(T)/CBS energies. The data show that most levels of theory have the highest errors for systems with butane dimers, and calculated interaction energies in these systems are overestimated. The best levels, overall for all systems, are BLYP-D3BJ/clef2-QZVP and BLYP-D3BJ/cc-pVQZ with similar root mean square deviation (RMSD) values of 0.056 kcal mo1-1 and 0.060 kcalmorl compared to CCSD(T) values. The best level for (1) 2-butene dimers is B3LYP-D3BJ/aug-cc-pVDZ; for (2) interactions between 2-butene and butane is BLYP-D3BJ/def2-SVP; while for (3) butane dimers is BLYP-D3BJ/def2-QZVP. The differences in calculated energies among several methods are not high, however, it is important that most of the DFT methods overestimate interactions in butane dimers.",
publisher = "Elsevier",
journal = "Computational and Theoretical Chemistry",
title = "Best methods for calculating interaction energies in 2-butene and butane systems",
volume = "1117",
pages = "150-161",
doi = "10.1016/j.comptc.2017.08.001"
}

Zarić, M., Bugarski, B.,& Kijevčanin, M.. (2017). Best methods for calculating interaction energies in 2-butene and butane systems. in Computational and Theoretical Chemistry
Elsevier., 1117, 150-161.
https://doi.org/10.1016/j.comptc.2017.08.001

Zarić M, Bugarski B, Kijevčanin M. Best methods for calculating interaction energies in 2-butene and butane systems. in Computational and Theoretical Chemistry. 2017;1117:150-161.
doi:10.1016/j.comptc.2017.08.001 .

Zarić, Milana, Bugarski, Branko, Kijevčanin, Mirjana, "Best methods for calculating interaction energies in 2-butene and butane systems" in Computational and Theoretical Chemistry, 1117 (2017):150-161,
https://doi.org/10.1016/j.comptc.2017.08.001 . .

TY  - CONF
AU  - Balaž, Ana Marija
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://www.bds.org.rs/en/conferences.php
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3279
AB  - Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium
 belongs to a group of oxidoreductases and has the ability to degrade different components
 of woody plants. CDH is secreted by wood degrading, phytopathogenic and saprotrophic
 fungi and this widespread appearance implies hers important function and makes her an
 important enzyme for applications in industrial and biotechnological processes, as well as
 biosensors and biofuel cells. CDH is also used in industry for bleaching cotton and in
 food industry for lactose detection. CDH is monomeric enzyme consisting of two
 domains, flavin domain containing FAD as cofactor and smaller hem b containing
 cytochrome domain, connected via flexible linker. Physiological role of CDH is reflected
 in the degradation of cellulose and lignin in cooperation with other cellulolytic enzymes,
 because CDH catalyzes oxidation of celobiose (Glc - β - 1,4 Glc) and other β - 1,4 - linked
 disaccharides and oligosaccharides to the corresponding lactons. Enzymes used in
 biosensors and for bleaching cotton should have high stability, especially toward reactive
 oxygen species. In order to improve oxidative stability of CDH, we have mutated CDH
 and tested its stability in presence of hydrogen peroxide. After successful cloning of the
 CDH gene in pYES2 vector, saturation mutagenesis was used to make library mutants
 where tree methionine residues were mutated. Residual activity of mutants was measured
 after the enzyme incubation in 0.3 M hydrogen peroxide for 0, 2 and 6h. After analysis of
 large number of mutants, it was observed that three mutants are showing higher oxidative
 stability compared to the wild - type enzyme. Residual activities of these mutants after 6
 hour incubation in the hydrogen peroxide were over 50%, whereas wild-type has 30%.
 Selected mutants were expressed in S. cerevisiae and purified on DEAE column. Purity
 and activity of the enzymes were detected on the electrophoresis gel, oxidative stability of
 purified mutants was measured once again and characterization of these mutants was done. Mutants showing increased oxidative stability were sequenced and we have decided to
 combine these mutations with each other in order to make combined mutants that will be
 tested for oxidative stability. Screening library mutants for improved features in
 microtitatar plates is a long time process, in order to shorten the time necessary for
 screening libraries with 106 mutants we are developing fluorescent assay for flou
 cytometry.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
T1  - Semi - rational design of cellobiose dehydrogenase from Phanerochaete chrysosporium for increased oxidative stability and high-throughput screening of library mutants
UR  - https://hdl.handle.net/21.15107/rcub_cer_3279
ER  - 

@conference{
author = "Balaž, Ana Marija and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2017",
abstract = "Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium
 belongs to a group of oxidoreductases and has the ability to degrade different components
 of woody plants. CDH is secreted by wood degrading, phytopathogenic and saprotrophic
 fungi and this widespread appearance implies hers important function and makes her an
 important enzyme for applications in industrial and biotechnological processes, as well as
 biosensors and biofuel cells. CDH is also used in industry for bleaching cotton and in
 food industry for lactose detection. CDH is monomeric enzyme consisting of two
 domains, flavin domain containing FAD as cofactor and smaller hem b containing
 cytochrome domain, connected via flexible linker. Physiological role of CDH is reflected
 in the degradation of cellulose and lignin in cooperation with other cellulolytic enzymes,
 because CDH catalyzes oxidation of celobiose (Glc - β - 1,4 Glc) and other β - 1,4 - linked
 disaccharides and oligosaccharides to the corresponding lactons. Enzymes used in
 biosensors and for bleaching cotton should have high stability, especially toward reactive
 oxygen species. In order to improve oxidative stability of CDH, we have mutated CDH
 and tested its stability in presence of hydrogen peroxide. After successful cloning of the
 CDH gene in pYES2 vector, saturation mutagenesis was used to make library mutants
 where tree methionine residues were mutated. Residual activity of mutants was measured
 after the enzyme incubation in 0.3 M hydrogen peroxide for 0, 2 and 6h. After analysis of
 large number of mutants, it was observed that three mutants are showing higher oxidative
 stability compared to the wild - type enzyme. Residual activities of these mutants after 6
 hour incubation in the hydrogen peroxide were over 50%, whereas wild-type has 30%.
 Selected mutants were expressed in S. cerevisiae and purified on DEAE column. Purity
 and activity of the enzymes were detected on the electrophoresis gel, oxidative stability of
 purified mutants was measured once again and characterization of these mutants was done. Mutants showing increased oxidative stability were sequenced and we have decided to
 combine these mutations with each other in order to make combined mutants that will be
 tested for oxidative stability. Screening library mutants for improved features in
 microtitatar plates is a long time process, in order to shorten the time necessary for
 screening libraries with 106 mutants we are developing fluorescent assay for flou
 cytometry.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings",
title = "Semi - rational design of cellobiose dehydrogenase from Phanerochaete chrysosporium for increased oxidative stability and high-throughput screening of library mutants",
url = "https://hdl.handle.net/21.15107/rcub_cer_3279"
}

Balaž, A. M., Ostafe, R., Fischer, R.,& Prodanović, R.. (2017). Semi - rational design of cellobiose dehydrogenase from Phanerochaete chrysosporium for increased oxidative stability and high-throughput screening of library mutants. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
Faculty of Chemistry, Serbian Biochemical Society..
https://hdl.handle.net/21.15107/rcub_cer_3279

Balaž AM, Ostafe R, Fischer R, Prodanović R. Semi - rational design of cellobiose dehydrogenase from Phanerochaete chrysosporium for increased oxidative stability and high-throughput screening of library mutants. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings. 2017;.
https://hdl.handle.net/21.15107/rcub_cer_3279 .

Balaž, Ana Marija, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Semi - rational design of cellobiose dehydrogenase from Phanerochaete chrysosporium for increased oxidative stability and high-throughput screening of library mutants" in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings (2017),
https://hdl.handle.net/21.15107/rcub_cer_3279 .

TY  - CONF
AU  - Zelenović, Jelena
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://www.bds.org.rs/en/conferences.php
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3282
AB  - Cellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
T1  - Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase
UR  - https://hdl.handle.net/21.15107/rcub_cer_3282
ER  - 

@conference{
author = "Zelenović, Jelena and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2017",
abstract = "Cellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings",
title = "Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase",
url = "https://hdl.handle.net/21.15107/rcub_cer_3282"
}

Zelenović, J., Ostafe, R., Fischer, R.,& Prodanović, R.. (2017). Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
Faculty of Chemistry, Serbian Biochemical Society..
https://hdl.handle.net/21.15107/rcub_cer_3282

Zelenović J, Ostafe R, Fischer R, Prodanović R. Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings. 2017;.
https://hdl.handle.net/21.15107/rcub_cer_3282 .

Zelenović, Jelena, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase" in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings (2017),
https://hdl.handle.net/21.15107/rcub_cer_3282 .

TY  - CONF
AU  - Zarić, Milana
AU  - Bugarski, Branko
AU  - Pavelkić, Vesna
AU  - Pantović Pavlović, Marijana
AU  - Stevanović, Jasmina
AU  - Pavlović, Miroslav
AU  - Kijevčanin, Mirjana
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6600
AB  - The interactions in model systems of unsaturated molecules with cis- and trans-double bonds were studied usingquantum chemical calculations. We present benchmark study of cis- and trans-2- butene dimer model systems with various methods and different basis sets. Root Mean Square Deviation (RMSD) values are represented and results showed that the best agreement with CCSD(T)/CBS values has B3LYP-D3 method with cc-pVTZ basis set, with the lowest RMSD value of 0.032.
PB  - Belgrade, Serbia : Society of Physical Chemists of Serbia
C3  - Proceedings - Physical Chemistry 2016 - 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, Belgrade
T1  - Benchmark study for 2-butene parallel interactions
VL  - 1
SP  - 75
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_cer_6600
ER  - 

@conference{
author = "Zarić, Milana and Bugarski, Branko and Pavelkić, Vesna and Pantović Pavlović, Marijana and Stevanović, Jasmina and Pavlović, Miroslav and Kijevčanin, Mirjana",
year = "2016",
abstract = "The interactions in model systems of unsaturated molecules with cis- and trans-double bonds were studied usingquantum chemical calculations. We present benchmark study of cis- and trans-2- butene dimer model systems with various methods and different basis sets. Root Mean Square Deviation (RMSD) values are represented and results showed that the best agreement with CCSD(T)/CBS values has B3LYP-D3 method with cc-pVTZ basis set, with the lowest RMSD value of 0.032.",
publisher = "Belgrade, Serbia : Society of Physical Chemists of Serbia",
journal = "Proceedings - Physical Chemistry 2016 - 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, Belgrade",
title = "Benchmark study for 2-butene parallel interactions",
volume = "1",
pages = "75-78",
url = "https://hdl.handle.net/21.15107/rcub_cer_6600"
}

Zarić, M., Bugarski, B., Pavelkić, V., Pantović Pavlović, M., Stevanović, J., Pavlović, M.,& Kijevčanin, M.. (2016). Benchmark study for 2-butene parallel interactions. in Proceedings - Physical Chemistry 2016 - 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, Belgrade
Belgrade, Serbia : Society of Physical Chemists of Serbia., 1, 75-78.
https://hdl.handle.net/21.15107/rcub_cer_6600

Zarić M, Bugarski B, Pavelkić V, Pantović Pavlović M, Stevanović J, Pavlović M, Kijevčanin M. Benchmark study for 2-butene parallel interactions. in Proceedings - Physical Chemistry 2016 - 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, Belgrade. 2016;1:75-78.
https://hdl.handle.net/21.15107/rcub_cer_6600 .

Zarić, Milana, Bugarski, Branko, Pavelkić, Vesna, Pantović Pavlović, Marijana, Stevanović, Jasmina, Pavlović, Miroslav, Kijevčanin, Mirjana, "Benchmark study for 2-butene parallel interactions" in Proceedings - Physical Chemistry 2016 - 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, Belgrade, 1 (2016):75-78,
https://hdl.handle.net/21.15107/rcub_cer_6600 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Žižak, Željko
AU  - Vukovic, Nebojsa
AU  - Šekularac, Gavrilo
AU  - Drmanić, Saša Ž.
AU  - Bugarski, Branko
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1966
AB  - Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.
PB  - Elsevier
T2  - Journal of Functional Foods
T1  - Biological potential of puffballs: A comparative analysis
VL  - 21
SP  - 36
EP  - 49
DO  - 10.1016/j.jff.2015.11.039
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, Jovana and Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Žižak, Željko and Vukovic, Nebojsa and Šekularac, Gavrilo and Drmanić, Saša Ž. and Bugarski, Branko",
year = "2016",
abstract = "Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.",
publisher = "Elsevier",
journal = "Journal of Functional Foods",
title = "Biological potential of puffballs: A comparative analysis",
volume = "21",
pages = "36-49",
doi = "10.1016/j.jff.2015.11.039"
}

Petrović, P., Vunduk, J., Klaus, A., Kozarski, M. S., Nikšić, M., Žižak, Ž., Vukovic, N., Šekularac, G., Drmanić, S. Ž.,& Bugarski, B.. (2016). Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods
Elsevier., 21, 36-49.
https://doi.org/10.1016/j.jff.2015.11.039

Petrović P, Vunduk J, Klaus A, Kozarski MS, Nikšić M, Žižak Ž, Vukovic N, Šekularac G, Drmanić SŽ, Bugarski B. Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods. 2016;21:36-49.
doi:10.1016/j.jff.2015.11.039 .

Petrović, Predrag, Vunduk, Jovana, Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Žižak, Željko, Vukovic, Nebojsa, Šekularac, Gavrilo, Drmanić, Saša Ž., Bugarski, Branko, "Biological potential of puffballs: A comparative analysis" in Journal of Functional Foods, 21 (2016):36-49,
https://doi.org/10.1016/j.jff.2015.11.039 . .

TY  - JOUR
AU  - Zarić, Milana
AU  - Bugarski, Branko
AU  - Kijevčanin, Mirjana
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1971
AB  - Noncovalent interactions of cis- and trans-2-butene, as the smallest model systems of molecules with cis and trans double bonds, were studied to find potential differences in interactions of these molecules. The study was performed using quantum chemical methods including very accurate CCSD(T)/CBS method. We studied parallel and displaced parallel interactions in 2-butene dimers, in butane dimers, and between 2-butene and saturated butane. The results show the trend that interactions of 2-butene with butane are the strongest, followed by interactions in butane dimers, whereas the interaction in 2-butene dimers are the weakest. The strongest calculated interaction energy is between trans-2-butene and butane, with a CCSD(T)/CBS energy of -2.80kcalmol(-1). Interactions in cis-2-butene dimers are stronger than interactions in trans-2-butene dimers. Interestingly, some of the interactions involving 2-butene are as strong as interactions in a benzene dimer. These insights into interactions of cis- and trans-2-butene can improve understanding of the properties and processes that involve molecules with cis and trans double bonds, such as fatty acids and polymers.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemphyschem
T1  - Interactions of Molecules with cis and trans Double Bonds: A Theoretical Study of cis- and trans-2-Butene
VL  - 17
IS  - 2
SP  - 317
EP  - 324
DO  - 10.1002/cphc.201500592
ER  - 

@article{
author = "Zarić, Milana and Bugarski, Branko and Kijevčanin, Mirjana",
year = "2016",
abstract = "Noncovalent interactions of cis- and trans-2-butene, as the smallest model systems of molecules with cis and trans double bonds, were studied to find potential differences in interactions of these molecules. The study was performed using quantum chemical methods including very accurate CCSD(T)/CBS method. We studied parallel and displaced parallel interactions in 2-butene dimers, in butane dimers, and between 2-butene and saturated butane. The results show the trend that interactions of 2-butene with butane are the strongest, followed by interactions in butane dimers, whereas the interaction in 2-butene dimers are the weakest. The strongest calculated interaction energy is between trans-2-butene and butane, with a CCSD(T)/CBS energy of -2.80kcalmol(-1). Interactions in cis-2-butene dimers are stronger than interactions in trans-2-butene dimers. Interestingly, some of the interactions involving 2-butene are as strong as interactions in a benzene dimer. These insights into interactions of cis- and trans-2-butene can improve understanding of the properties and processes that involve molecules with cis and trans double bonds, such as fatty acids and polymers.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemphyschem",
title = "Interactions of Molecules with cis and trans Double Bonds: A Theoretical Study of cis- and trans-2-Butene",
volume = "17",
number = "2",
pages = "317-324",
doi = "10.1002/cphc.201500592"
}

Zarić, M., Bugarski, B.,& Kijevčanin, M.. (2016). Interactions of Molecules with cis and trans Double Bonds: A Theoretical Study of cis- and trans-2-Butene. in Chemphyschem
Wiley-V C H Verlag Gmbh, Weinheim., 17(2), 317-324.
https://doi.org/10.1002/cphc.201500592

Zarić M, Bugarski B, Kijevčanin M. Interactions of Molecules with cis and trans Double Bonds: A Theoretical Study of cis- and trans-2-Butene. in Chemphyschem. 2016;17(2):317-324.
doi:10.1002/cphc.201500592 .

Zarić, Milana, Bugarski, Branko, Kijevčanin, Mirjana, "Interactions of Molecules with cis and trans Double Bonds: A Theoretical Study of cis- and trans-2-Butene" in Chemphyschem, 17, no. 2 (2016):317-324,
https://doi.org/10.1002/cphc.201500592 . .

TY  - JOUR
AU  - Rabrenovic, Biljana B
AU  - Vujasinović, Vesna B.
AU  - Novaković, Miroslav
AU  - Corbo, Selma C
AU  - Basic, Zorica N.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2008
AB  - The objective of this study was to investigate the nutritional value of seven samples of cold pressed pumpkin oil of different origins and influence of seed origin on the content of the most important bioactive components. Four samples of a pumpkin oil is obtained by cold pressing of the seeds of domestic and Austrian varieties, and three samples of cold pressed oils were obtained from the seeds of unknown origin, taken by free choice in the market. As indicators of the nutritional values are determined by the composition and content of fatty acids, tocopherols and sterols. In the composition of the fatty acid were oleic dominant (34.2 +/- 0.09-43.9 +/- 0.04%) and linolenic fatty acid (30.8 +/- 0.09-46.9 +/- 0.015%). This study confirmed that the oil pumpkin dominant beta+gamma-tocopherol, whose contents ranged from 34.65 +/- 0.03 to 44.59 +/- 0.69 mg/100 g. We determine the composition and content of Delta(7)-phytosterols, especially for specific oil pumpkins. It was detected five Delta(7)-sterols: spinasterol, Delta(7,22,25)-stigmastatrienol, Delta(7,25)-stigmastadienol, Delta(7)-stigmasterol and Delta(7)-avenasterol. Dominant content was Delta(7,22)-stigmastadienol or spinasterol with 39.98 to 50.31% of the total content of sterols.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Comparative review of the nutritional value of cold-pressed pumpkin (cucurbita pepo l.) Seed oil of different origins
VL  - 70
IS  - 1
SP  - 59
EP  - 65
DO  - 10.2298/HEMIND141128011R
ER  - 

@article{
author = "Rabrenovic, Biljana B and Vujasinović, Vesna B. and Novaković, Miroslav and Corbo, Selma C and Basic, Zorica N.",
year = "2016",
abstract = "The objective of this study was to investigate the nutritional value of seven samples of cold pressed pumpkin oil of different origins and influence of seed origin on the content of the most important bioactive components. Four samples of a pumpkin oil is obtained by cold pressing of the seeds of domestic and Austrian varieties, and three samples of cold pressed oils were obtained from the seeds of unknown origin, taken by free choice in the market. As indicators of the nutritional values are determined by the composition and content of fatty acids, tocopherols and sterols. In the composition of the fatty acid were oleic dominant (34.2 +/- 0.09-43.9 +/- 0.04%) and linolenic fatty acid (30.8 +/- 0.09-46.9 +/- 0.015%). This study confirmed that the oil pumpkin dominant beta+gamma-tocopherol, whose contents ranged from 34.65 +/- 0.03 to 44.59 +/- 0.69 mg/100 g. We determine the composition and content of Delta(7)-phytosterols, especially for specific oil pumpkins. It was detected five Delta(7)-sterols: spinasterol, Delta(7,22,25)-stigmastatrienol, Delta(7,25)-stigmastadienol, Delta(7)-stigmasterol and Delta(7)-avenasterol. Dominant content was Delta(7,22)-stigmastadienol or spinasterol with 39.98 to 50.31% of the total content of sterols.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Comparative review of the nutritional value of cold-pressed pumpkin (cucurbita pepo l.) Seed oil of different origins",
volume = "70",
number = "1",
pages = "59-65",
doi = "10.2298/HEMIND141128011R"
}

Rabrenovic, B. B., Vujasinović, V. B., Novaković, M., Corbo, S. C.,& Basic, Z. N.. (2016). Comparative review of the nutritional value of cold-pressed pumpkin (cucurbita pepo l.) Seed oil of different origins. in Hemijska industrija
Association of Chemical Engineers of Serbia., 70(1), 59-65.
https://doi.org/10.2298/HEMIND141128011R

Rabrenovic BB, Vujasinović VB, Novaković M, Corbo SC, Basic ZN. Comparative review of the nutritional value of cold-pressed pumpkin (cucurbita pepo l.) Seed oil of different origins. in Hemijska industrija. 2016;70(1):59-65.
doi:10.2298/HEMIND141128011R .

Rabrenovic, Biljana B, Vujasinović, Vesna B., Novaković, Miroslav, Corbo, Selma C, Basic, Zorica N., "Comparative review of the nutritional value of cold-pressed pumpkin (cucurbita pepo l.) Seed oil of different origins" in Hemijska industrija, 70, no. 1 (2016):59-65,
https://doi.org/10.2298/HEMIND141128011R . .

TY  - JOUR
AU  - Levic, Steva
AU  - Lijakovic, Ivana Pajic
AU  - Dordevic, Verica
AU  - Rac, Vladislav
AU  - Rakić, Vesna M.
AU  - Šolević Knudsen, Tatjana
AU  - Pavlović, Vladimir B.
AU  - Bugarski, Branko
AU  - Nedovic, Viktor
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3173
AB  - In this study, calcium alginate beads immobilizing D-limonene (solid systems) have been manufactured starting from emulsions of this flavor in sodium alginate (liquid systems). The effects of alginate concentration (0.02 and 0.03 g/mL) and flavor content (5 and 10 % w/w) on viscosity, conductivity and stability of emulsions were investigated. The flavor droplets in emulsions are bigger as polymer solution is more concentrated and contains more of the flavor. When emulsions have been subjected to electrostatic extrusion and upon Na+-Ca2+ ion exchange, smaller (similar to 960 to similar to 1450 mm) and less spherical beads were obtained (sphericity factor 0.003-0.21) compared to beads produced by simple dripping technique (without electrostatic field). When wet beads were air dried, they shrunk less if they had higher content of the flavor. Novel mathematical model describing swelling kinetics of dried beads is developed. In this work, D-limonene was efficiently immobilized within Ca-alginate beads (immobilization efficiency similar to 50 to similar to 77%) and its thermal stability was confirmed by TG/MS analysis.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Hydrocolloids
T1  - Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion
VL  - 45
SP  - 111
EP  - 123
DO  - 10.1016/j.foodhyd.2014.10.001
ER  - 

@article{
author = "Levic, Steva and Lijakovic, Ivana Pajic and Dordevic, Verica and Rac, Vladislav and Rakić, Vesna M. and Šolević Knudsen, Tatjana and Pavlović, Vladimir B. and Bugarski, Branko and Nedovic, Viktor",
year = "2015",
abstract = "In this study, calcium alginate beads immobilizing D-limonene (solid systems) have been manufactured starting from emulsions of this flavor in sodium alginate (liquid systems). The effects of alginate concentration (0.02 and 0.03 g/mL) and flavor content (5 and 10 % w/w) on viscosity, conductivity and stability of emulsions were investigated. The flavor droplets in emulsions are bigger as polymer solution is more concentrated and contains more of the flavor. When emulsions have been subjected to electrostatic extrusion and upon Na+-Ca2+ ion exchange, smaller (similar to 960 to similar to 1450 mm) and less spherical beads were obtained (sphericity factor 0.003-0.21) compared to beads produced by simple dripping technique (without electrostatic field). When wet beads were air dried, they shrunk less if they had higher content of the flavor. Novel mathematical model describing swelling kinetics of dried beads is developed. In this work, D-limonene was efficiently immobilized within Ca-alginate beads (immobilization efficiency similar to 50 to similar to 77%) and its thermal stability was confirmed by TG/MS analysis.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Hydrocolloids",
title = "Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion",
volume = "45",
pages = "111-123",
doi = "10.1016/j.foodhyd.2014.10.001"
}

Levic, S., Lijakovic, I. P., Dordevic, V., Rac, V., Rakić, V. M., Šolević Knudsen, T., Pavlović, V. B., Bugarski, B.,& Nedovic, V.. (2015). Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion. in Food Hydrocolloids
Elsevier Sci Ltd, Oxford., 45, 111-123.
https://doi.org/10.1016/j.foodhyd.2014.10.001

Levic S, Lijakovic IP, Dordevic V, Rac V, Rakić VM, Šolević Knudsen T, Pavlović VB, Bugarski B, Nedovic V. Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion. in Food Hydrocolloids. 2015;45:111-123.
doi:10.1016/j.foodhyd.2014.10.001 .

Levic, Steva, Lijakovic, Ivana Pajic, Dordevic, Verica, Rac, Vladislav, Rakić, Vesna M., Šolević Knudsen, Tatjana, Pavlović, Vladimir B., Bugarski, Branko, Nedovic, Viktor, "Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion" in Food Hydrocolloids, 45 (2015):111-123,
https://doi.org/10.1016/j.foodhyd.2014.10.001 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Žižak, Željko
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1666
AB  - The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
VL  - 6
IS  - 6
SP  - 1875
EP  - 1886
DO  - 10.1039/c5fo00312a
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Vunduk, Jovana and Žižak, Željko and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms",
volume = "6",
number = "6",
pages = "1875-1886",
doi = "10.1039/c5fo00312a"
}

Kozarski, M. S., Klaus, A., Vunduk, J., Žižak, Ž., Nikšić, M., Jakovljević, D., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function
Royal Soc Chemistry, Cambridge., 6(6), 1875-1886.
https://doi.org/10.1039/c5fo00312a

Kozarski MS, Klaus A, Vunduk J, Žižak Ž, Nikšić M, Jakovljević D, Vrvić M, Van Griensven LJLD. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function. 2015;6(6):1875-1886.
doi:10.1039/c5fo00312a .

Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Žižak, Željko, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms" in Food & Function, 6, no. 6 (2015):1875-1886,
https://doi.org/10.1039/c5fo00312a . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Vunduk, Jovana
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - Žižak, Željko
AU  - Pavlović, Vladimir B.
AU  - Levic, Steva
AU  - Nikšić, Miomir
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1761
AB  - Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.
PB  - Elsevier
T2  - Food Research International
T1  - Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa
VL  - 67
SP  - 272
EP  - 283
DO  - 10.1016/j.foodres.2014.11.035
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Vunduk, Jovana and Todorović, Nina and Jakovljević, Dragica and Žižak, Željko and Pavlović, Vladimir B. and Levic, Steva and Nikšić, Miomir and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa",
volume = "67",
pages = "272-283",
doi = "10.1016/j.foodres.2014.11.035"
}

Klaus, A., Kozarski, M. S., Vunduk, J., Todorović, N., Jakovljević, D., Žižak, Ž., Pavlović, V. B., Levic, S., Nikšić, M.,& Van Griensven, L. J. L. D.. (2015). Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International
Elsevier., 67, 272-283.
https://doi.org/10.1016/j.foodres.2014.11.035

Klaus A, Kozarski MS, Vunduk J, Todorović N, Jakovljević D, Žižak Ž, Pavlović VB, Levic S, Nikšić M, Van Griensven LJLD. Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International. 2015;67:272-283.
doi:10.1016/j.foodres.2014.11.035 .

Klaus, Anita, Kozarski, Maja S., Vunduk, Jovana, Todorović, Nina, Jakovljević, Dragica, Žižak, Željko, Pavlović, Vladimir B., Levic, Steva, Nikšić, Miomir, Van Griensven, Leo J. L. D., "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa" in Food Research International, 67 (2015):272-283,
https://doi.org/10.1016/j.foodres.2014.11.035 . .

TY  - JOUR
AU  - Levic, Steva
AU  - Lijakovic, Ivana Pajic
AU  - Dordevic, Verica
AU  - Rac, Vladislav
AU  - Rakić, Vesna M.
AU  - Šolević Knudsen, Tatjana
AU  - Pavlović, Vladimir B.
AU  - Bugarski, Branko
AU  - Nedovic, Viktor
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1762
AB  - In this study, calcium alginate beads immobilizing D-limonene (solid systems) have been manufactured starting from emulsions of this flavor in sodium alginate (liquid systems). The effects of alginate concentration (0.02 and 0.03 g/mL) and flavor content (5 and 10 % w/w) on viscosity, conductivity and stability of emulsions were investigated. The flavor droplets in emulsions are bigger as polymer solution is more concentrated and contains more of the flavor. When emulsions have been subjected to electrostatic extrusion and upon Na+-Ca2+ ion exchange, smaller (similar to 960 to similar to 1450 mm) and less spherical beads were obtained (sphericity factor 0.003-0.21) compared to beads produced by simple dripping technique (without electrostatic field). When wet beads were air dried, they shrunk less if they had higher content of the flavor. Novel mathematical model describing swelling kinetics of dried beads is developed. In this work, D-limonene was efficiently immobilized within Ca-alginate beads (immobilization efficiency similar to 50 to similar to 77%) and its thermal stability was confirmed by TG/MS analysis.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Hydrocolloids
T1  - Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion
VL  - 45
SP  - 111
EP  - 123
DO  - 10.1016/j.foodhyd.2014.10.001
ER  - 

@article{
author = "Levic, Steva and Lijakovic, Ivana Pajic and Dordevic, Verica and Rac, Vladislav and Rakić, Vesna M. and Šolević Knudsen, Tatjana and Pavlović, Vladimir B. and Bugarski, Branko and Nedovic, Viktor",
year = "2015",
abstract = "In this study, calcium alginate beads immobilizing D-limonene (solid systems) have been manufactured starting from emulsions of this flavor in sodium alginate (liquid systems). The effects of alginate concentration (0.02 and 0.03 g/mL) and flavor content (5 and 10 % w/w) on viscosity, conductivity and stability of emulsions were investigated. The flavor droplets in emulsions are bigger as polymer solution is more concentrated and contains more of the flavor. When emulsions have been subjected to electrostatic extrusion and upon Na+-Ca2+ ion exchange, smaller (similar to 960 to similar to 1450 mm) and less spherical beads were obtained (sphericity factor 0.003-0.21) compared to beads produced by simple dripping technique (without electrostatic field). When wet beads were air dried, they shrunk less if they had higher content of the flavor. Novel mathematical model describing swelling kinetics of dried beads is developed. In this work, D-limonene was efficiently immobilized within Ca-alginate beads (immobilization efficiency similar to 50 to similar to 77%) and its thermal stability was confirmed by TG/MS analysis.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Hydrocolloids",
title = "Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion",
volume = "45",
pages = "111-123",
doi = "10.1016/j.foodhyd.2014.10.001"
}

Levic, S., Lijakovic, I. P., Dordevic, V., Rac, V., Rakić, V. M., Šolević Knudsen, T., Pavlović, V. B., Bugarski, B.,& Nedovic, V.. (2015). Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion. in Food Hydrocolloids
Elsevier Sci Ltd, Oxford., 45, 111-123.
https://doi.org/10.1016/j.foodhyd.2014.10.001

Levic S, Lijakovic IP, Dordevic V, Rac V, Rakić VM, Šolević Knudsen T, Pavlović VB, Bugarski B, Nedovic V. Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion. in Food Hydrocolloids. 2015;45:111-123.
doi:10.1016/j.foodhyd.2014.10.001 .

Levic, Steva, Lijakovic, Ivana Pajic, Dordevic, Verica, Rac, Vladislav, Rakić, Vesna M., Šolević Knudsen, Tatjana, Pavlović, Vladimir B., Bugarski, Branko, Nedovic, Viktor, "Characterization of sodium alginate/D-limonene emulsions and respective calcium alginate/D-limonene beads produced by electrostatic extrusion" in Food Hydrocolloids, 45 (2015):111-123,
https://doi.org/10.1016/j.foodhyd.2014.10.001 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1826
AB  - Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.
PB  - MDPI
T2  - Molecules
T1  - Antioxidants of Edible Mushrooms
VL  - 20
IS  - 10
SP  - 19489
EP  - 19525
DO  - 10.3390/molecules201019489
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Vunduk, Jovana and Petrović, Predrag and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.",
publisher = "MDPI",
journal = "Molecules",
title = "Antioxidants of Edible Mushrooms",
volume = "20",
number = "10",
pages = "19489-19525",
doi = "10.3390/molecules201019489"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Vunduk, J., Petrović, P., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Antioxidants of Edible Mushrooms. in Molecules
MDPI., 20(10), 19489-19525.
https://doi.org/10.3390/molecules201019489

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Vunduk J, Petrović P, Nikšić M, Vrvić M, Van Griensven LJLD. Antioxidants of Edible Mushrooms. in Molecules. 2015;20(10):19489-19525.
doi:10.3390/molecules201019489 .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Vunduk, Jovana, Petrović, Predrag, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Antioxidants of Edible Mushrooms" in Molecules, 20, no. 10 (2015):19489-19525,
https://doi.org/10.3390/molecules201019489 . .