TY  - JOUR
AU  - Jovanovic, Aleksandra A.
AU  - Dordevic, Verica B.
AU  - Zdunić, Gordana
AU  - Pljevljakusic, Dejan
AU  - Savikin, Katarina P.
AU  - Gođevac, Dejan
AU  - Bugarski, Branko
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2217
AB  - Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.
PB  - Elsevier
T2  - Separation and Purification Technology
T1  - Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques
VL  - 179
SP  - 369
EP  - 380
DO  - 10.1016/j.seppur.2017.01.055
ER  - 

@article{
author = "Jovanovic, Aleksandra A. and Dordevic, Verica B. and Zdunić, Gordana and Pljevljakusic, Dejan and Savikin, Katarina P. and Gođevac, Dejan and Bugarski, Branko",
year = "2017",
abstract = "Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.",
publisher = "Elsevier",
journal = "Separation and Purification Technology",
title = "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques",
volume = "179",
pages = "369-380",
doi = "10.1016/j.seppur.2017.01.055"
}

Jovanovic, A. A., Dordevic, V. B., Zdunić, G., Pljevljakusic, D., Savikin, K. P., Gođevac, D.,& Bugarski, B.. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology
Elsevier., 179, 369-380.
https://doi.org/10.1016/j.seppur.2017.01.055

Jovanovic AA, Dordevic VB, Zdunić G, Pljevljakusic D, Savikin KP, Gođevac D, Bugarski B. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology. 2017;179:369-380.
doi:10.1016/j.seppur.2017.01.055 .

Jovanovic, Aleksandra A., Dordevic, Verica B., Zdunić, Gordana, Pljevljakusic, Dejan, Savikin, Katarina P., Gođevac, Dejan, Bugarski, Branko, "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques" in Separation and Purification Technology, 179 (2017):369-380,
https://doi.org/10.1016/j.seppur.2017.01.055 . .

TY  - JOUR
AU  - Jovanovic, Aleksandra A.
AU  - Dordevic, Verica B.
AU  - Zdunić, Gordana
AU  - Pljevljakusic, Dejan
AU  - Savikin, Katarina P.
AU  - Gođevac, Dejan
AU  - Bugarski, Branko
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3005
AB  - Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.
PB  - Elsevier
T2  - Separation and Purification Technology
T1  - Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques
VL  - 179
SP  - 369
EP  - 380
DO  - 10.1016/j.seppur.2017.01.055
ER  - 

@article{
author = "Jovanovic, Aleksandra A. and Dordevic, Verica B. and Zdunić, Gordana and Pljevljakusic, Dejan and Savikin, Katarina P. and Gođevac, Dejan and Bugarski, Branko",
year = "2017",
abstract = "Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE > HAE > maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.",
publisher = "Elsevier",
journal = "Separation and Purification Technology",
title = "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques",
volume = "179",
pages = "369-380",
doi = "10.1016/j.seppur.2017.01.055"
}

Jovanovic, A. A., Dordevic, V. B., Zdunić, G., Pljevljakusic, D., Savikin, K. P., Gođevac, D.,& Bugarski, B.. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology
Elsevier., 179, 369-380.
https://doi.org/10.1016/j.seppur.2017.01.055

Jovanovic AA, Dordevic VB, Zdunić G, Pljevljakusic D, Savikin KP, Gođevac D, Bugarski B. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology. 2017;179:369-380.
doi:10.1016/j.seppur.2017.01.055 .

Jovanovic, Aleksandra A., Dordevic, Verica B., Zdunić, Gordana, Pljevljakusic, Dejan, Savikin, Katarina P., Gođevac, Dejan, Bugarski, Branko, "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques" in Separation and Purification Technology, 179 (2017):369-380,
https://doi.org/10.1016/j.seppur.2017.01.055 . .

TY  - JOUR
AU  - Savikin, Katarina
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Gođevac, Dejan
AU  - Stanojković, Tatjana
AU  - Pljevljakusic, Dejan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1584
AB  - A detailed phenolic composition analysis of dry chokeberry, bilberry and black currant fruit teas prepared on the most common ways-decoction and infusion, was performed, together with evaluation of cytotoxic activity. The most abundant in phenolics were chokeberry teas, followed by bilberry and black currant teas, while the highest anthocyanin amount was determined in bilberry samples. LC/DAD/MS method was used for identification of 17 anthocyanins, 11 flavonoids and 4 hydroxycinnamic acid derivatives. Quantitative analysis of investigated teas was carried out by HPLC analysis, and major phenolic compounds in berry fruit teas were chlorogenic acids, quercetin glycosides and anthocyanin glycosides. Berry teas were investigated for cytotoxic activity against cervix carcinoma, melanoma, colon, and chronic myelogenous leukemia cell lines, and chokebeny decoction was the most effective against all cell lines. According to the results obtained in this study, pure chokeberry, bilberry and black currant teas could be valuable sources of flavonoids and anthocyanins.
PB  - Elsevier
T2  - Food Research International
T1  - Berry fruit teas: Phenolic composition and cytotoxic activity
VL  - 62
SP  - 677
EP  - 683
DO  - 10.1016/j.foodres.2014.04.017
ER  - 

@article{
author = "Savikin, Katarina and Zdunić, Gordana and Janković, Teodora and Gođevac, Dejan and Stanojković, Tatjana and Pljevljakusic, Dejan",
year = "2014",
abstract = "A detailed phenolic composition analysis of dry chokeberry, bilberry and black currant fruit teas prepared on the most common ways-decoction and infusion, was performed, together with evaluation of cytotoxic activity. The most abundant in phenolics were chokeberry teas, followed by bilberry and black currant teas, while the highest anthocyanin amount was determined in bilberry samples. LC/DAD/MS method was used for identification of 17 anthocyanins, 11 flavonoids and 4 hydroxycinnamic acid derivatives. Quantitative analysis of investigated teas was carried out by HPLC analysis, and major phenolic compounds in berry fruit teas were chlorogenic acids, quercetin glycosides and anthocyanin glycosides. Berry teas were investigated for cytotoxic activity against cervix carcinoma, melanoma, colon, and chronic myelogenous leukemia cell lines, and chokebeny decoction was the most effective against all cell lines. According to the results obtained in this study, pure chokeberry, bilberry and black currant teas could be valuable sources of flavonoids and anthocyanins.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Berry fruit teas: Phenolic composition and cytotoxic activity",
volume = "62",
pages = "677-683",
doi = "10.1016/j.foodres.2014.04.017"
}

Savikin, K., Zdunić, G., Janković, T., Gođevac, D., Stanojković, T.,& Pljevljakusic, D.. (2014). Berry fruit teas: Phenolic composition and cytotoxic activity. in Food Research International
Elsevier., 62, 677-683.
https://doi.org/10.1016/j.foodres.2014.04.017

Savikin K, Zdunić G, Janković T, Gođevac D, Stanojković T, Pljevljakusic D. Berry fruit teas: Phenolic composition and cytotoxic activity. in Food Research International. 2014;62:677-683.
doi:10.1016/j.foodres.2014.04.017 .

Savikin, Katarina, Zdunić, Gordana, Janković, Teodora, Gođevac, Dejan, Stanojković, Tatjana, Pljevljakusic, Dejan, "Berry fruit teas: Phenolic composition and cytotoxic activity" in Food Research International, 62 (2014):677-683,
https://doi.org/10.1016/j.foodres.2014.04.017 . .

TY  - JOUR
AU  - Pljevljakusic, Dejan
AU  - Janković, Teodora
AU  - Jelačić, Slavica C.
AU  - Novaković, Miroslav
AU  - Menković, Nebojša
AU  - Beatovic, Damir
AU  - Dajić-Stevanović, Zora
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1536
AB  - Arnica (Arnica montana L) is a perennial mountain plant whose flower heads are mainly used for topical treatment of bruises and sprains in phytopharmaceutical preparations such as ointments, creams and gels. In this study, different models of arnica cultivation have been tested in three-year field trial arranged in 2 x 3 x 2 factorial design. Planting date (spring and autumn), fertilizater type (manure, NPK and control) and mode of reproduction (generative and clonal) were induced factors on the trial. The variation of the rosette diameter, flowering stem count and height, flower heads count and diameter, together with flower heads yield and chemical properties were investigated as morphological parameters. Spring planting of clonally propagated plants was showed to be the least successful model due to the low percent of rooted plants and weak shoot development. Other models yielded from 59.8 to 143.6 kg ha(-1) and 116.3 to 258.7 kg ha(-1) flower heads (dw) in the second and third year, respectively. Total sesquiterpene lactones content in flower heads of all tested variants was above the minimum of pharmacopeia quality regulation and ranged from 4.6 to 13.9 mg g(-1) depending on factor levels, while the amounts of two dominant flavonoid compounds quercetin-3-O-glucoside and kaempferol-3-O-glucoside ranged from 7.8 to 13.9 mg g(-1) and from 2.1 to 4.7 mg g(-1), respectively. All induced factors in the field trial significantly influenced variation in both morphological and chemical parameters, where fertilized variants from autumn planting have shown to be superior in flower heads yield with satisfying levels of the secondary metabolites content. Factorial analysis distinguished that half of morphological parameters positively correlated with flower heads yield. First three ranks of sequential classification of cultivation models with desirable properties were captured by variants planted in autumn, while the last rated variants were those from clonal propagation planted in spring. This attempt of arnica domestication in agroecological conditions of western Serbia has proven to be successful in ensuring high quality raw material, providing at the same time answers to the questions that have not been reviewed so far.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Morphological and chemical characterization of Arnica montana L. under different cultivation models
VL  - 52
SP  - 233
EP  - 244
DO  - 10.1016/j.indcrop.2013.10.035
ER  - 

@article{
author = "Pljevljakusic, Dejan and Janković, Teodora and Jelačić, Slavica C. and Novaković, Miroslav and Menković, Nebojša and Beatovic, Damir and Dajić-Stevanović, Zora",
year = "2014",
abstract = "Arnica (Arnica montana L) is a perennial mountain plant whose flower heads are mainly used for topical treatment of bruises and sprains in phytopharmaceutical preparations such as ointments, creams and gels. In this study, different models of arnica cultivation have been tested in three-year field trial arranged in 2 x 3 x 2 factorial design. Planting date (spring and autumn), fertilizater type (manure, NPK and control) and mode of reproduction (generative and clonal) were induced factors on the trial. The variation of the rosette diameter, flowering stem count and height, flower heads count and diameter, together with flower heads yield and chemical properties were investigated as morphological parameters. Spring planting of clonally propagated plants was showed to be the least successful model due to the low percent of rooted plants and weak shoot development. Other models yielded from 59.8 to 143.6 kg ha(-1) and 116.3 to 258.7 kg ha(-1) flower heads (dw) in the second and third year, respectively. Total sesquiterpene lactones content in flower heads of all tested variants was above the minimum of pharmacopeia quality regulation and ranged from 4.6 to 13.9 mg g(-1) depending on factor levels, while the amounts of two dominant flavonoid compounds quercetin-3-O-glucoside and kaempferol-3-O-glucoside ranged from 7.8 to 13.9 mg g(-1) and from 2.1 to 4.7 mg g(-1), respectively. All induced factors in the field trial significantly influenced variation in both morphological and chemical parameters, where fertilized variants from autumn planting have shown to be superior in flower heads yield with satisfying levels of the secondary metabolites content. Factorial analysis distinguished that half of morphological parameters positively correlated with flower heads yield. First three ranks of sequential classification of cultivation models with desirable properties were captured by variants planted in autumn, while the last rated variants were those from clonal propagation planted in spring. This attempt of arnica domestication in agroecological conditions of western Serbia has proven to be successful in ensuring high quality raw material, providing at the same time answers to the questions that have not been reviewed so far.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Morphological and chemical characterization of Arnica montana L. under different cultivation models",
volume = "52",
pages = "233-244",
doi = "10.1016/j.indcrop.2013.10.035"
}

Pljevljakusic, D., Janković, T., Jelačić, S. C., Novaković, M., Menković, N., Beatovic, D.,& Dajić-Stevanović, Z.. (2014). Morphological and chemical characterization of Arnica montana L. under different cultivation models. in Industrial Crops and Products
Elsevier., 52, 233-244.
https://doi.org/10.1016/j.indcrop.2013.10.035

Pljevljakusic D, Janković T, Jelačić SC, Novaković M, Menković N, Beatovic D, Dajić-Stevanović Z. Morphological and chemical characterization of Arnica montana L. under different cultivation models. in Industrial Crops and Products. 2014;52:233-244.
doi:10.1016/j.indcrop.2013.10.035 .

Pljevljakusic, Dejan, Janković, Teodora, Jelačić, Slavica C., Novaković, Miroslav, Menković, Nebojša, Beatovic, Damir, Dajić-Stevanović, Zora, "Morphological and chemical characterization of Arnica montana L. under different cultivation models" in Industrial Crops and Products, 52 (2014):233-244,
https://doi.org/10.1016/j.indcrop.2013.10.035 . .

TY  - JOUR
AU  - Pljevljakusic, Dejan
AU  - Savikin, Katarina
AU  - Janković, Teodora
AU  - Zdunić, Gordana
AU  - Ristic, Mihailo
AU  - Gođevac, Dejan
AU  - Konic-Ristic, Aleksandra
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/867
AB  - Phytochemical analyses of the cultivated Sideritis raeseri subsp. raeseri in four different stages of flower development were performed. Traditionally used infusion and decoction were also prepared from aerial parts in full flowering stage, and analyses of active compounds and radical scavenging capacity were performed. The highest yield of the essential oil, obtained by hydrodistillation, was noticed in the full flowering phase (0.11%), with sesquiterpene bicyclogermacrene as the main constituent (42.5%). All examined extracts contained phenolic compounds and their amounts varied from 15.3 to 34.1 mg GAE/g DW. The amounts of total phenolics in infusion and decoction were similar (46.5 and 43.9 mg GAE/100 ml, respectively). LC-ESI-MS analyses of all samples allowed the characterisation of 22 phenolic compounds. Two dominant flavone glycosides, 4'-O-methylhypolaetin-7-O-[6"'-O-acetyl-beta-D-allopyranosyl (1 -> 2)-beta-D-glucopyranoside (17) and 4'-O-methylisoscutellarein-7-O-[6"'-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-beta-D-glucopyranoside (19) were quantified using HPLC. Moreover, the mineral content and the percent of transportation were investigated.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp raeseri
VL  - 124
IS  - 1
SP  - 226
EP  - 233
DO  - 10.1016/j.foodchem.2010.06.023
ER  - 

@article{
author = "Pljevljakusic, Dejan and Savikin, Katarina and Janković, Teodora and Zdunić, Gordana and Ristic, Mihailo and Gođevac, Dejan and Konic-Ristic, Aleksandra",
year = "2011",
abstract = "Phytochemical analyses of the cultivated Sideritis raeseri subsp. raeseri in four different stages of flower development were performed. Traditionally used infusion and decoction were also prepared from aerial parts in full flowering stage, and analyses of active compounds and radical scavenging capacity were performed. The highest yield of the essential oil, obtained by hydrodistillation, was noticed in the full flowering phase (0.11%), with sesquiterpene bicyclogermacrene as the main constituent (42.5%). All examined extracts contained phenolic compounds and their amounts varied from 15.3 to 34.1 mg GAE/g DW. The amounts of total phenolics in infusion and decoction were similar (46.5 and 43.9 mg GAE/100 ml, respectively). LC-ESI-MS analyses of all samples allowed the characterisation of 22 phenolic compounds. Two dominant flavone glycosides, 4'-O-methylhypolaetin-7-O-[6"'-O-acetyl-beta-D-allopyranosyl (1 -> 2)-beta-D-glucopyranoside (17) and 4'-O-methylisoscutellarein-7-O-[6"'-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-beta-D-glucopyranoside (19) were quantified using HPLC. Moreover, the mineral content and the percent of transportation were investigated.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp raeseri",
volume = "124",
number = "1",
pages = "226-233",
doi = "10.1016/j.foodchem.2010.06.023"
}

Pljevljakusic, D., Savikin, K., Janković, T., Zdunić, G., Ristic, M., Gođevac, D.,& Konic-Ristic, A.. (2011). Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp raeseri. in Food Chemistry
Elsevier Sci Ltd, Oxford., 124(1), 226-233.
https://doi.org/10.1016/j.foodchem.2010.06.023

Pljevljakusic D, Savikin K, Janković T, Zdunić G, Ristic M, Gođevac D, Konic-Ristic A. Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp raeseri. in Food Chemistry. 2011;124(1):226-233.
doi:10.1016/j.foodchem.2010.06.023 .

Pljevljakusic, Dejan, Savikin, Katarina, Janković, Teodora, Zdunić, Gordana, Ristic, Mihailo, Gođevac, Dejan, Konic-Ristic, Aleksandra, "Chemical properties of the cultivated Sideritis raeseri Boiss. & Heldr. subsp raeseri" in Food Chemistry, 124, no. 1 (2011):226-233,
https://doi.org/10.1016/j.foodchem.2010.06.023 . .