TY  - JOUR
AU  - Jaćimović, Simona
AU  - Kiprovski, Biljana
AU  - Ristivojević, Petar
AU  - Dimić, Dušan
AU  - Nakarada, Đura
AU  - Dojčinović, Biljana
AU  - Sikora, Vladimir
AU  - Teslić, Nemanja
AU  - Pantelić, Nebojša
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7194
AB  - Sorghum grain (Sorghum bicolor L. Moench) is a gluten-free cereal with excellent nutritional value and is a good source of antioxidants, including polyphenols, as well as minerals with proven health benefits. Herein, the phenolic composition, elemental profile, and antioxidant activity of sixteen food-grade sorghum grains (S1–S16) grown under agroecological conditions in Serbia were determined. Nine phenolic compounds characteristic of sorghum grains, such as luteolinidin, 5-methoxyluteolinidin, luteolidin derivative, luteolidin glucoside, apigeninidin, 7-methoxyapigeninidin, apigeninidin glucoside, and cyanidin derivative, were quantified. The antioxidant potential of the analyzed sorghum grains was evaluated by UV/Vis (DPPH, ABTS, and FRAP) and Electron Paramagnetic Resonance spectroscopy (hydroxyl and ascorbyl radical scavenging assays). The content of macro- and microelements was determined by Inductively Coupled Plasma Optical Emission spectroscopy. Theoretical daily intakes of selected major and trace elements were assessed and compared with the Recommended Daily Allowance or Adequate Intake. Sample S8 had the highest amount of phenolic compounds, while S4, S6, and S8 exhibited the strongest antioxidative potential. The sorghum studied could completely satisfy the daily needs of macro- (K, Mg, and P) and microelements (Se, Zn, Fe). Pattern recognition techniques confirmed the discrimination of samples based on phenolic profile and elemental analysis and recognized the main markers responsible for differences between the investigated samples. The reaction between hydroxyl radicals and luteolinidin/apigeninidin was investigated by Density Functional Theory and thermodynamically preferred mechanism was determined.
PB  - MDPI
T2  - Antioxidants
T2  - Antioxidants
T1  - Chemical Composition, Antioxidant Potential, and Nutritional Evaluation of Cultivated Sorghum Grains: A Combined Experimental, Theoretical, and Multivariate Analysis
VL  - 12
IS  - 8
SP  - 1485
DO  - 10.3390/antiox12081485
ER  - 

@article{
author = "Jaćimović, Simona and Kiprovski, Biljana and Ristivojević, Petar and Dimić, Dušan and Nakarada, Đura and Dojčinović, Biljana and Sikora, Vladimir and Teslić, Nemanja and Pantelić, Nebojša",
year = "2023",
abstract = "Sorghum grain (Sorghum bicolor L. Moench) is a gluten-free cereal with excellent nutritional value and is a good source of antioxidants, including polyphenols, as well as minerals with proven health benefits. Herein, the phenolic composition, elemental profile, and antioxidant activity of sixteen food-grade sorghum grains (S1–S16) grown under agroecological conditions in Serbia were determined. Nine phenolic compounds characteristic of sorghum grains, such as luteolinidin, 5-methoxyluteolinidin, luteolidin derivative, luteolidin glucoside, apigeninidin, 7-methoxyapigeninidin, apigeninidin glucoside, and cyanidin derivative, were quantified. The antioxidant potential of the analyzed sorghum grains was evaluated by UV/Vis (DPPH, ABTS, and FRAP) and Electron Paramagnetic Resonance spectroscopy (hydroxyl and ascorbyl radical scavenging assays). The content of macro- and microelements was determined by Inductively Coupled Plasma Optical Emission spectroscopy. Theoretical daily intakes of selected major and trace elements were assessed and compared with the Recommended Daily Allowance or Adequate Intake. Sample S8 had the highest amount of phenolic compounds, while S4, S6, and S8 exhibited the strongest antioxidative potential. The sorghum studied could completely satisfy the daily needs of macro- (K, Mg, and P) and microelements (Se, Zn, Fe). Pattern recognition techniques confirmed the discrimination of samples based on phenolic profile and elemental analysis and recognized the main markers responsible for differences between the investigated samples. The reaction between hydroxyl radicals and luteolinidin/apigeninidin was investigated by Density Functional Theory and thermodynamically preferred mechanism was determined.",
publisher = "MDPI",
journal = "Antioxidants, Antioxidants",
title = "Chemical Composition, Antioxidant Potential, and Nutritional Evaluation of Cultivated Sorghum Grains: A Combined Experimental, Theoretical, and Multivariate Analysis",
volume = "12",
number = "8",
pages = "1485",
doi = "10.3390/antiox12081485"
}

Jaćimović, S., Kiprovski, B., Ristivojević, P., Dimić, D., Nakarada, Đ., Dojčinović, B., Sikora, V., Teslić, N.,& Pantelić, N.. (2023). Chemical Composition, Antioxidant Potential, and Nutritional Evaluation of Cultivated Sorghum Grains: A Combined Experimental, Theoretical, and Multivariate Analysis. in Antioxidants
MDPI., 12(8), 1485.
https://doi.org/10.3390/antiox12081485

Jaćimović S, Kiprovski B, Ristivojević P, Dimić D, Nakarada Đ, Dojčinović B, Sikora V, Teslić N, Pantelić N. Chemical Composition, Antioxidant Potential, and Nutritional Evaluation of Cultivated Sorghum Grains: A Combined Experimental, Theoretical, and Multivariate Analysis. in Antioxidants. 2023;12(8):1485.
doi:10.3390/antiox12081485 .

Jaćimović, Simona, Kiprovski, Biljana, Ristivojević, Petar, Dimić, Dušan, Nakarada, Đura, Dojčinović, Biljana, Sikora, Vladimir, Teslić, Nemanja, Pantelić, Nebojša, "Chemical Composition, Antioxidant Potential, and Nutritional Evaluation of Cultivated Sorghum Grains: A Combined Experimental, Theoretical, and Multivariate Analysis" in Antioxidants, 12, no. 8 (2023):1485,
https://doi.org/10.3390/antiox12081485 . .

TY  - JOUR
AU  - Ivanović, Stefan
AU  - Gođevac, Dejan
AU  - Ristivojević, Petar
AU  - Zdunić, Gordana
AU  - Stojanović, Danilo
AU  - Šavikin, Katarina
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5648
AB  - Introduction: Chokeberry is a plant belonging to the family Rosaceae which has a long history of edible and medicinal use. The aims of this study are: i) to investigate of HPTLC profile of the berry samples using HPTLC technique based metabolomics approach, ii) to determine the botanical origin of investigated chokeberry adulterations iii) to identify the most important botanical markers responsible for classification. Methods: A simple, rapid, and high-throughput planar chromatography (HPTLC) method was applied in combination with multivariate techniques for differentiating chokeberry samples and identification of the main metabolites of chokeberry and four common adulterants. Images of HPTLC chromatograms obtained using two different detections i.e. visible light and derivatization with natural products reagent were used as a dataset for multivariate analysis. Results: Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of five botanically different samples and recognized their main markers such as responsible for differences between chokeberry and four common adulterants. Conclusion: The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the detection of chokeberry adulteration.
PB  - Elsevier GmbH
T2  - Journal of Herbal Medicine
T1  - HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration
VL  - 37
SP  - 100618
DO  - 10.1016/j.hermed.2022.100618
ER  - 

@article{
author = "Ivanović, Stefan and Gođevac, Dejan and Ristivojević, Petar and Zdunić, Gordana and Stojanović, Danilo and Šavikin, Katarina",
year = "2023",
abstract = "Introduction: Chokeberry is a plant belonging to the family Rosaceae which has a long history of edible and medicinal use. The aims of this study are: i) to investigate of HPTLC profile of the berry samples using HPTLC technique based metabolomics approach, ii) to determine the botanical origin of investigated chokeberry adulterations iii) to identify the most important botanical markers responsible for classification. Methods: A simple, rapid, and high-throughput planar chromatography (HPTLC) method was applied in combination with multivariate techniques for differentiating chokeberry samples and identification of the main metabolites of chokeberry and four common adulterants. Images of HPTLC chromatograms obtained using two different detections i.e. visible light and derivatization with natural products reagent were used as a dataset for multivariate analysis. Results: Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of five botanically different samples and recognized their main markers such as responsible for differences between chokeberry and four common adulterants. Conclusion: The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the detection of chokeberry adulteration.",
publisher = "Elsevier GmbH",
journal = "Journal of Herbal Medicine",
title = "HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration",
volume = "37",
pages = "100618",
doi = "10.1016/j.hermed.2022.100618"
}

Ivanović, S., Gođevac, D., Ristivojević, P., Zdunić, G., Stojanović, D.,& Šavikin, K.. (2023). HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration. in Journal of Herbal Medicine
Elsevier GmbH., 37, 100618.
https://doi.org/10.1016/j.hermed.2022.100618

Ivanović S, Gođevac D, Ristivojević P, Zdunić G, Stojanović D, Šavikin K. HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration. in Journal of Herbal Medicine. 2023;37:100618.
doi:10.1016/j.hermed.2022.100618 .

Ivanović, Stefan, Gođevac, Dejan, Ristivojević, Petar, Zdunić, Gordana, Stojanović, Danilo, Šavikin, Katarina, "HPTLC-based metabolomics approach for the detection of chokeberry (Aronia melanocarpa (Michx.) Elliott) adulteration" in Journal of Herbal Medicine, 37 (2023):100618,
https://doi.org/10.1016/j.hermed.2022.100618 . .

TY  - CONF
AU  - Mutić, Tijana
AU  - Stanković, Vesna
AU  - Ristivojević, Petar
AU  - Stanković, Dalibor
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7322
AB  - In this work, nickel oxide (NiO) nanoparticles were synthesized by the chemical coprecipitation method and used for modification of the carbon paste electrode for GA detection and determination. Morphological properties of prepared material were investigated using ICP-OES (inductively coupled plasma–optical emission spectrometry), XRD (X-ray diffraction), SEM and TEM (Scanning and Transmission Electron Microscopy). The electrochemical properties of the prepared electrode and the behavior of GA over the modified electrode were examined using CV (Cyclic Voltammetry), EIS (Electrochemical impedance spectroscopy), and SWV (Square Wave 
Voltammetry).
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of abstracts, 4th November 2023, Novi Sad, Serbia
T1  - Preparation of NiO supported carbon paste electrode for sensitive and selective determination of Gallic acid in plant samples
SP  - 125
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_cer_7322
ER  - 

@conference{
author = "Mutić, Tijana and Stanković, Vesna and Ristivojević, Petar and Stanković, Dalibor",
year = "2023",
abstract = "In this work, nickel oxide (NiO) nanoparticles were synthesized by the chemical coprecipitation method and used for modification of the carbon paste electrode for GA detection and determination. Morphological properties of prepared material were investigated using ICP-OES (inductively coupled plasma–optical emission spectrometry), XRD (X-ray diffraction), SEM and TEM (Scanning and Transmission Electron Microscopy). The electrochemical properties of the prepared electrode and the behavior of GA over the modified electrode were examined using CV (Cyclic Voltammetry), EIS (Electrochemical impedance spectroscopy), and SWV (Square Wave 
Voltammetry).",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of abstracts, 4th November 2023, Novi Sad, Serbia",
title = "Preparation of NiO supported carbon paste electrode for sensitive and selective determination of Gallic acid in plant samples",
pages = "125-125",
url = "https://hdl.handle.net/21.15107/rcub_cer_7322"
}

Mutić, T., Stanković, V., Ristivojević, P.,& Stanković, D.. (2023). Preparation of NiO supported carbon paste electrode for sensitive and selective determination of Gallic acid in plant samples. in 9th Conference of Young Chemists of Serbia, Book of abstracts, 4th November 2023, Novi Sad, Serbia
Belgrade : Serbian Chemical Society., 125-125.
https://hdl.handle.net/21.15107/rcub_cer_7322

Mutić T, Stanković V, Ristivojević P, Stanković D. Preparation of NiO supported carbon paste electrode for sensitive and selective determination of Gallic acid in plant samples. in 9th Conference of Young Chemists of Serbia, Book of abstracts, 4th November 2023, Novi Sad, Serbia. 2023;:125-125.
https://hdl.handle.net/21.15107/rcub_cer_7322 .

Mutić, Tijana, Stanković, Vesna, Ristivojević, Petar, Stanković, Dalibor, "Preparation of NiO supported carbon paste electrode for sensitive and selective determination of Gallic acid in plant samples" in 9th Conference of Young Chemists of Serbia, Book of abstracts, 4th November 2023, Novi Sad, Serbia (2023):125-125,
https://hdl.handle.net/21.15107/rcub_cer_7322 .

TY  - CONF
AU  - Ivanović, Stefan
AU  - Ristivojević, Petar
AU  - Zdunić, Gordana
AU  - Stojanović, Danilo
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7537
AB  - Chokeberry is a plant belonging to the family Rosaceae which has a long history of edible and
medicinal use. The aims of this study are: i) to investigate of HPTLC profile of berries samples
using HPTLC technique based metabolomics approach, ii) to determine the botanical origin of
investigated chokeberry adulterations iii) to identify the most important botanical markers
responsible for classification.
A simple, rapid, and high-throughput planar chromatography (HPTLC) method was applied in
combination with multivariate techniques for differentiating chokeberry samples and identification
of the main metabolites of chokeberry and four common adulterants. Images of HPTLC
chromatograms obtained using two different detections i.e. visible light and derivatization with
natural products reagent were used as a dataset for multivariate analysis.
Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis
confirmed the discrimination of five botanically different samples and recognized their main
markers such as responsible for differences between chokeberry and four common adulterants.
The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the detection of chokeberry adulteration
PB  - University of Belgrade
C3  - Unifood Conference, Program i zbornik radova, Beograd, 24 i 25 septembar 2021. / Unifood Conference, Programme & Book of Abstracts, Belgrade September 24-25 2021
T1  - Detection of chokeberry adulteration by HPTLS-based metabolomics
SP  - 136
EP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_cer_7537
ER  - 

@conference{
author = "Ivanović, Stefan and Ristivojević, Petar and Zdunić, Gordana and Stojanović, Danilo and Šavikin, Katarina and Gođevac, Dejan",
year = "2021",
abstract = "Chokeberry is a plant belonging to the family Rosaceae which has a long history of edible and
medicinal use. The aims of this study are: i) to investigate of HPTLC profile of berries samples
using HPTLC technique based metabolomics approach, ii) to determine the botanical origin of
investigated chokeberry adulterations iii) to identify the most important botanical markers
responsible for classification.
A simple, rapid, and high-throughput planar chromatography (HPTLC) method was applied in
combination with multivariate techniques for differentiating chokeberry samples and identification
of the main metabolites of chokeberry and four common adulterants. Images of HPTLC
chromatograms obtained using two different detections i.e. visible light and derivatization with
natural products reagent were used as a dataset for multivariate analysis.
Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis
confirmed the discrimination of five botanically different samples and recognized their main
markers such as responsible for differences between chokeberry and four common adulterants.
The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the detection of chokeberry adulteration",
publisher = "University of Belgrade",
journal = "Unifood Conference, Program i zbornik radova, Beograd, 24 i 25 septembar 2021. / Unifood Conference, Programme & Book of Abstracts, Belgrade September 24-25 2021",
title = "Detection of chokeberry adulteration by HPTLS-based metabolomics",
pages = "136-136",
url = "https://hdl.handle.net/21.15107/rcub_cer_7537"
}

Ivanović, S., Ristivojević, P., Zdunić, G., Stojanović, D., Šavikin, K.,& Gođevac, D.. (2021). Detection of chokeberry adulteration by HPTLS-based metabolomics. in Unifood Conference, Program i zbornik radova, Beograd, 24 i 25 septembar 2021. / Unifood Conference, Programme & Book of Abstracts, Belgrade September 24-25 2021
University of Belgrade., 136-136.
https://hdl.handle.net/21.15107/rcub_cer_7537

Ivanović S, Ristivojević P, Zdunić G, Stojanović D, Šavikin K, Gođevac D. Detection of chokeberry adulteration by HPTLS-based metabolomics. in Unifood Conference, Program i zbornik radova, Beograd, 24 i 25 septembar 2021. / Unifood Conference, Programme & Book of Abstracts, Belgrade September 24-25 2021. 2021;:136-136.
https://hdl.handle.net/21.15107/rcub_cer_7537 .

Ivanović, Stefan, Ristivojević, Petar, Zdunić, Gordana, Stojanović, Danilo, Šavikin, Katarina, Gođevac, Dejan, "Detection of chokeberry adulteration by HPTLS-based metabolomics" in Unifood Conference, Program i zbornik radova, Beograd, 24 i 25 septembar 2021. / Unifood Conference, Programme & Book of Abstracts, Belgrade September 24-25 2021 (2021):136-136,
https://hdl.handle.net/21.15107/rcub_cer_7537 .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Živković-Radovanović, Vukosava
AU  - Tosti, Tomislav
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4770
AB  - Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants.
PB  - USA :Taylor & Francis INC
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Thin-layer chromatography in bioassays of antimicrobial compounds from plants
DO  - 10.1080/10826076.2021.1968429
ER  - 

@article{
author = "Šegan, Sandra and Živković-Radovanović, Vukosava and Tosti, Tomislav and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2021",
abstract = "Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants.",
publisher = "USA :Taylor & Francis INC",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Thin-layer chromatography in bioassays of antimicrobial compounds from plants",
doi = "10.1080/10826076.2021.1968429"
}

Šegan, S., Živković-Radovanović, V., Tosti, T., Ristivojević, P.,& Milojković-Opsenica, D.. (2021). Thin-layer chromatography in bioassays of antimicrobial compounds from plants. in Journal of Liquid Chromatography & Related Technologies
USA :Taylor & Francis INC..
https://doi.org/10.1080/10826076.2021.1968429

Šegan S, Živković-Radovanović V, Tosti T, Ristivojević P, Milojković-Opsenica D. Thin-layer chromatography in bioassays of antimicrobial compounds from plants. in Journal of Liquid Chromatography & Related Technologies. 2021;.
doi:10.1080/10826076.2021.1968429 .

Šegan, Sandra, Živković-Radovanović, Vukosava, Tosti, Tomislav, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Thin-layer chromatography in bioassays of antimicrobial compounds from plants" in Journal of Liquid Chromatography & Related Technologies (2021),
https://doi.org/10.1080/10826076.2021.1968429 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Čomić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2932
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3139
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
VL  - 222
SP  - 148
EP  - 158
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Čomić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojković-Opsenica, Dušanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
volume = "222",
pages = "148-158",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Čomić, L. R., Vasiljević, B., Opsenica, D., Milojković-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Čomić LR, Vasiljević B, Opsenica D, Milojković-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Čomić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojković-Opsenica, Dušanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojevic, Petar
AU  - Pavić, Aleksandar
AU  - Radojevic, Ivana
AU  - Čomić, Ljiljana R.
AU  - Vasiljevic, Branka
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
AU  - Senerovic, Lidija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2310
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
VL  - 222
SP  - 148
EP  - 158
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojevic, Petar and Pavić, Aleksandar and Radojevic, Ivana and Čomić, Ljiljana R. and Vasiljevic, Branka and Opsenica, Dejan and Milojković-Opsenica, Dušanka and Senerovic, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
volume = "222",
pages = "148-158",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojevic, P., Pavić, A., Radojevic, I., Čomić, L. R., Vasiljevic, B., Opsenica, D., Milojković-Opsenica, D.,& Senerovic, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojevic P, Pavić A, Radojevic I, Čomić LR, Vasiljevic B, Opsenica D, Milojković-Opsenica D, Senerovic L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojevic, Petar, Pavić, Aleksandar, Radojevic, Ivana, Čomić, Ljiljana R., Vasiljevic, Branka, Opsenica, Dejan, Milojković-Opsenica, Dušanka, Senerovic, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .