TY  - JOUR
AU  - Ljujić, Jovana
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Sofrenić, Ivana V.
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Anđelković, Boban
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7574
AB  - Data processing and data extraction are the first, and most often crucial, steps in metabolomics and multivariate data analysis in general. There are several software solutions for these purposes in GC-MS metabolomics. It becomes unclear which platform offers what kind of data and how that information influences the analysis’s conclusions. In this study, selected analytical platforms for GC-MS metabolomics profiling, SpectConnect and XCMS as well as MestReNova software, were used to process the results of the HS-SPME/GC-MS aroma analyses of several blackberry varieties. In addition, a detailed analysis of the identification of the individual components of the blackberry aroma club varieties was performed. In total, 72 components were detected in the XCMS platform, 119 in SpectConnect, and 87 and 167 in MestReNova, with automatic integral and manual correction, respectively, as well as 219 aroma components after manual analysis of GC-MS chromatograms. The obtained datasets were fed, for multivariate data analysis, to SIMCA software, and underwent the creation of PCA, OPLS, and OPLS-DA models. The results of the validation tests and VIP-pred. scores were analyzed in detail.
PB  - MDPI
T2  - Foods
T1  - Critical Review of Selected Analytical Platforms for GC-MS Metabolomics Profiling—Case Study: HS-SPME/GC-MS Analysis of Blackberry’s Aroma
VL  - 13
IS  - 8
SP  - 1222
DO  - 10.3390/foods13081222
ER  - 

@article{
author = "Ljujić, Jovana and Vujisić, Ljubodrag and Tešević, Vele and Sofrenić, Ivana V. and Ivanović, Stefan and Simić, Katarina and Anđelković, Boban",
year = "2024",
abstract = "Data processing and data extraction are the first, and most often crucial, steps in metabolomics and multivariate data analysis in general. There are several software solutions for these purposes in GC-MS metabolomics. It becomes unclear which platform offers what kind of data and how that information influences the analysis’s conclusions. In this study, selected analytical platforms for GC-MS metabolomics profiling, SpectConnect and XCMS as well as MestReNova software, were used to process the results of the HS-SPME/GC-MS aroma analyses of several blackberry varieties. In addition, a detailed analysis of the identification of the individual components of the blackberry aroma club varieties was performed. In total, 72 components were detected in the XCMS platform, 119 in SpectConnect, and 87 and 167 in MestReNova, with automatic integral and manual correction, respectively, as well as 219 aroma components after manual analysis of GC-MS chromatograms. The obtained datasets were fed, for multivariate data analysis, to SIMCA software, and underwent the creation of PCA, OPLS, and OPLS-DA models. The results of the validation tests and VIP-pred. scores were analyzed in detail.",
publisher = "MDPI",
journal = "Foods",
title = "Critical Review of Selected Analytical Platforms for GC-MS Metabolomics Profiling—Case Study: HS-SPME/GC-MS Analysis of Blackberry’s Aroma",
volume = "13",
number = "8",
pages = "1222",
doi = "10.3390/foods13081222"
}

Ljujić, J., Vujisić, L., Tešević, V., Sofrenić, I. V., Ivanović, S., Simić, K.,& Anđelković, B.. (2024). Critical Review of Selected Analytical Platforms for GC-MS Metabolomics Profiling—Case Study: HS-SPME/GC-MS Analysis of Blackberry’s Aroma. in Foods
MDPI., 13(8), 1222.
https://doi.org/10.3390/foods13081222

Ljujić J, Vujisić L, Tešević V, Sofrenić IV, Ivanović S, Simić K, Anđelković B. Critical Review of Selected Analytical Platforms for GC-MS Metabolomics Profiling—Case Study: HS-SPME/GC-MS Analysis of Blackberry’s Aroma. in Foods. 2024;13(8):1222.
doi:10.3390/foods13081222 .

Ljujić, Jovana, Vujisić, Ljubodrag, Tešević, Vele, Sofrenić, Ivana V., Ivanović, Stefan, Simić, Katarina, Anđelković, Boban, "Critical Review of Selected Analytical Platforms for GC-MS Metabolomics Profiling—Case Study: HS-SPME/GC-MS Analysis of Blackberry’s Aroma" in Foods, 13, no. 8 (2024):1222,
https://doi.org/10.3390/foods13081222 . .

TY  - JOUR
AU  - Salbashian, M.
AU  - Stefanova, N.
AU  - Tzaneva, M.
AU  - Pavlov, D.
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Georgieva, A.
AU  - Eftimov, M.
AU  - Reyzov, M.
AU  - Nikolova, M.
AU  - Valcheva-Kuzmanova, S.
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7759
AB  - Skip to content IOP Science home Accessibility Help Search Journals Books Publishing Support Login IOP Conference Series: Earth and Environmental Science Purpose-led Publishing, find out more.    PAPER • THE FOLLOWING ARTICLE ISOPEN ACCESS Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage M Salbashian1, N Stefanova2, M Tzaneva2, D Pavlov3, M Novakovic4, V Tesevic5, A Georgieva1, M Eftimov1, M Reyzov1, M Nikolova2Show full author list  Published under licence by IOP Publishing Ltd IOP Conference Series: Earth and Environmental Science, Volume 1305, International Conference: Kliment's Days 2023 - 60 years Faculty of Biology 09/11/2023 - 11/11/2023 Sofia, Bulgaria Citation M Salbashian et al 2024 IOP Conf. Ser.: Earth Environ. Sci. 1305 012017 DOI 10.1088/1755-1315/1305/1/012017 DownloadArticle PDF References Download PDF Article metrics 91 Total downloads Share this article Article and author information Abstract Paracetamol (PCM)-induced toxicity is a well-established pharmacological model. Cotinus coggygria is a medicinal plant rich in polyphenols, including the flavonoid fustin. The aim of the present study was to evaluate the effects of the flavonoid fustin isolated from Cotinus coggygria heartwood in a model of PCM-induced liver damage. Male Wistar rats (n=48) were allocated to four groups: Control, PCM, F5+PCM, F10+PCM. The rodents were treated daily orally for nine consecutive days as follows: groups F5+PCM and F10+PCM – with fustin (suspended in a vehicle) at doses of 5 and 10 mg/kg, respectively; groups Control and PCM – with the vehicle. PCM was injected intraperitoneally (1.0 g/kg) on day 7. At the end of the experiment, serum and liver samples were prepared. PCM caused a severe liver damage confirmed by histopathological, immunohistochemical and biochemical indices. Compared to PCM group, in F5+PCM and F10+PCM groups, the hepatic necrosis, steatosis, ballooning degeneration, inflammation and expression of NF-κB were significantly reduced. Fustin treatment resulted in a significant reduction of serum activities of alanine aminotransferase and gamma-glutamyl transferase to levels that did not differ from the control values. The present study demonstrated a hepatoprotective effect of the flavonoid fustin in a rat model of acute paracetamol-induced toxicity.
PB  - IOP Publishing
T2  - IOP Conference Series: Earth and Environmental Science
T1  - Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage
VL  - 1305
IS  - 1
SP  - 012017
DO  - 10.1088/1755-1315/1305/1/012017
ER  - 

@article{
author = "Salbashian, M. and Stefanova, N. and Tzaneva, M. and Pavlov, D. and Novaković, Miroslav and Tešević, Vele and Georgieva, A. and Eftimov, M. and Reyzov, M. and Nikolova, M. and Valcheva-Kuzmanova, S.",
year = "2024",
abstract = "Skip to content IOP Science home Accessibility Help Search Journals Books Publishing Support Login IOP Conference Series: Earth and Environmental Science Purpose-led Publishing, find out more.    PAPER • THE FOLLOWING ARTICLE ISOPEN ACCESS Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage M Salbashian1, N Stefanova2, M Tzaneva2, D Pavlov3, M Novakovic4, V Tesevic5, A Georgieva1, M Eftimov1, M Reyzov1, M Nikolova2Show full author list  Published under licence by IOP Publishing Ltd IOP Conference Series: Earth and Environmental Science, Volume 1305, International Conference: Kliment's Days 2023 - 60 years Faculty of Biology 09/11/2023 - 11/11/2023 Sofia, Bulgaria Citation M Salbashian et al 2024 IOP Conf. Ser.: Earth Environ. Sci. 1305 012017 DOI 10.1088/1755-1315/1305/1/012017 DownloadArticle PDF References Download PDF Article metrics 91 Total downloads Share this article Article and author information Abstract Paracetamol (PCM)-induced toxicity is a well-established pharmacological model. Cotinus coggygria is a medicinal plant rich in polyphenols, including the flavonoid fustin. The aim of the present study was to evaluate the effects of the flavonoid fustin isolated from Cotinus coggygria heartwood in a model of PCM-induced liver damage. Male Wistar rats (n=48) were allocated to four groups: Control, PCM, F5+PCM, F10+PCM. The rodents were treated daily orally for nine consecutive days as follows: groups F5+PCM and F10+PCM – with fustin (suspended in a vehicle) at doses of 5 and 10 mg/kg, respectively; groups Control and PCM – with the vehicle. PCM was injected intraperitoneally (1.0 g/kg) on day 7. At the end of the experiment, serum and liver samples were prepared. PCM caused a severe liver damage confirmed by histopathological, immunohistochemical and biochemical indices. Compared to PCM group, in F5+PCM and F10+PCM groups, the hepatic necrosis, steatosis, ballooning degeneration, inflammation and expression of NF-κB were significantly reduced. Fustin treatment resulted in a significant reduction of serum activities of alanine aminotransferase and gamma-glutamyl transferase to levels that did not differ from the control values. The present study demonstrated a hepatoprotective effect of the flavonoid fustin in a rat model of acute paracetamol-induced toxicity.",
publisher = "IOP Publishing",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage",
volume = "1305",
number = "1",
pages = "012017",
doi = "10.1088/1755-1315/1305/1/012017"
}

Salbashian, M., Stefanova, N., Tzaneva, M., Pavlov, D., Novaković, M., Tešević, V., Georgieva, A., Eftimov, M., Reyzov, M., Nikolova, M.,& Valcheva-Kuzmanova, S.. (2024). Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage. in IOP Conference Series: Earth and Environmental Science
IOP Publishing., 1305(1), 012017.
https://doi.org/10.1088/1755-1315/1305/1/012017

Salbashian M, Stefanova N, Tzaneva M, Pavlov D, Novaković M, Tešević V, Georgieva A, Eftimov M, Reyzov M, Nikolova M, Valcheva-Kuzmanova S. Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage. in IOP Conference Series: Earth and Environmental Science. 2024;1305(1):012017.
doi:10.1088/1755-1315/1305/1/012017 .

Salbashian, M., Stefanova, N., Tzaneva, M., Pavlov, D., Novaković, Miroslav, Tešević, Vele, Georgieva, A., Eftimov, M., Reyzov, M., Nikolova, M., Valcheva-Kuzmanova, S., "Hepatoprotective effect of the flavonoid fustin in a rat model of paracetamol-induced acute liver damage" in IOP Conference Series: Earth and Environmental Science, 1305, no. 1 (2024):012017,
https://doi.org/10.1088/1755-1315/1305/1/012017 . .

TY  - JOUR
AU  - Eftimov, M
AU  - Georgieva, A
AU  - Pavlov, D
AU  - Todorova, M
AU  - Reyzov, M
AU  - Nikolova, M
AU  - Stefanova, N
AU  - Tsaneva, M
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Valcheva-Kuzmanova, S
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7758
AB  - Trinitrobenzene sulfonic acid (TNBS)-induced colitis is a widely used animal model that mimics the signs and symptoms of inflammatory bowel disease. Fustin is a flavonoid found in Cotinus coggygria.  The aim of the present study was to investigate the effect of fustin isolated from Cotinus coggygria heartwood in a rat model of TNBS-induced colitis.  In this experiment, 30 male Wistar rats were used, allocated to three groups: Control, TNBS and TNBS+F10. Colitis was induced by rectal application of TNBS. After the induction of colitis, fustin at a dose of 10 mg/kg was administered orally to TNBS+F10 group in the course of 8 days. Severity of colitis, oxidative stress and inflammation were assessed by macroscopic, histopathological, immunohistochemical and biochemical analyses.  Rats from TNBS group demonstrated severe colonic damage. Fustin treatment ameliorated most of the macroscopic and some of the histopathological indices of colonic damage, and restored the activity of the endogenous antioxidant superoxide dismutase in tissue homogenate but did not affect the signs of inflammation measured by the activity of alkaline phosphatase in serum and tissue homogenate, as well as the expression of NF-kB in the colon. In conclusion, the ameliorative effects of fustin in the experimental TNBS-induced colitis might be the result of its antioxidant properties.
PB  - IOP Publishing
T2  - IOP Conference Series: Earth and Environmental Science
T1  - Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis
VL  - 1305
IS  - 1
SP  - 012018
DO  - 10.1088/1755-1315/1305/1/012018
ER  - 

@article{
author = "Eftimov, M and Georgieva, A and Pavlov, D and Todorova, M and Reyzov, M and Nikolova, M and Stefanova, N and Tsaneva, M and Novaković, Miroslav and Tešević, Vele and Valcheva-Kuzmanova, S",
year = "2024",
abstract = "Trinitrobenzene sulfonic acid (TNBS)-induced colitis is a widely used animal model that mimics the signs and symptoms of inflammatory bowel disease. Fustin is a flavonoid found in Cotinus coggygria.  The aim of the present study was to investigate the effect of fustin isolated from Cotinus coggygria heartwood in a rat model of TNBS-induced colitis.  In this experiment, 30 male Wistar rats were used, allocated to three groups: Control, TNBS and TNBS+F10. Colitis was induced by rectal application of TNBS. After the induction of colitis, fustin at a dose of 10 mg/kg was administered orally to TNBS+F10 group in the course of 8 days. Severity of colitis, oxidative stress and inflammation were assessed by macroscopic, histopathological, immunohistochemical and biochemical analyses.  Rats from TNBS group demonstrated severe colonic damage. Fustin treatment ameliorated most of the macroscopic and some of the histopathological indices of colonic damage, and restored the activity of the endogenous antioxidant superoxide dismutase in tissue homogenate but did not affect the signs of inflammation measured by the activity of alkaline phosphatase in serum and tissue homogenate, as well as the expression of NF-kB in the colon. In conclusion, the ameliorative effects of fustin in the experimental TNBS-induced colitis might be the result of its antioxidant properties.",
publisher = "IOP Publishing",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis",
volume = "1305",
number = "1",
pages = "012018",
doi = "10.1088/1755-1315/1305/1/012018"
}

Eftimov, M., Georgieva, A., Pavlov, D., Todorova, M., Reyzov, M., Nikolova, M., Stefanova, N., Tsaneva, M., Novaković, M., Tešević, V.,& Valcheva-Kuzmanova, S.. (2024). Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis. in IOP Conference Series: Earth and Environmental Science
IOP Publishing., 1305(1), 012018.
https://doi.org/10.1088/1755-1315/1305/1/012018

Eftimov M, Georgieva A, Pavlov D, Todorova M, Reyzov M, Nikolova M, Stefanova N, Tsaneva M, Novaković M, Tešević V, Valcheva-Kuzmanova S. Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis. in IOP Conference Series: Earth and Environmental Science. 2024;1305(1):012018.
doi:10.1088/1755-1315/1305/1/012018 .

Eftimov, M, Georgieva, A, Pavlov, D, Todorova, M, Reyzov, M, Nikolova, M, Stefanova, N, Tsaneva, M, Novaković, Miroslav, Tešević, Vele, Valcheva-Kuzmanova, S, "Ameliorative effects of the flavonoid fustin in a rat model of trinitrobenzensulfonic acid-induced colitis" in IOP Conference Series: Earth and Environmental Science, 1305, no. 1 (2024):012018,
https://doi.org/10.1088/1755-1315/1305/1/012018 . .

TY  - JOUR
AU  - Simić, Katarina
AU  - Miladinović, Zoran
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Avramović, Nataša
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Gođevac, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Tasic, Ljubica
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6682
AB  - Bipolar disorder (BD) is a brain disorder that causes changes in a person’s mood, energy, and ability to function. It has a prevalence of 60 million people worldwide, and it is among the top 20 diseases with the highest global burden. The complexity of this disease, including diverse genetic, environmental, and biochemical factors, and diagnoses based on the subjective recognition of symptoms without any clinical test of biomarker identification create significant difficulties in understanding and diagnosing BD. A 1H-NMR-based metabolomic study applying chemometrics of serum samples of Serbian patients with BD (33) and healthy controls (39) was explored, providing the identification of 22 metabolites for this disease. A biomarker set including threonine, aspartate, gamma-aminobutyric acid, 2-hydroxybutyric acid, serine, and mannose was established for the first time in BD serum samples by an NMR-based metabolomics study. Six identified metabolites (3-hydroxybutyric acid, arginine, lysine, tyrosine, phenylalanine, and glycerol) are in agreement with the previously determined NMR-based sets of serum biomarkers in Brazilian and/or Chinese patient samples. The same established metabolites (lactate, alanine, valine, leucine, isoleucine, glutamine, glutamate, glucose, and choline) in three different ethnic and geographic origins (Serbia, Brazil, and China) might have a crucial role in the realization of a universal set of NMR biomarkers for BD.
PB  - MDPI AG
T2  - Metabolites
T1  - Metabolomic Profiling of Bipolar Disorder by 1H-NMR in Serbian Patients
VL  - 13
IS  - 5
SP  - 607
DO  - 10.3390/metabo13050607
ER  - 

@article{
author = "Simić, Katarina and Miladinović, Zoran and Todorović, Nina and Trifunović, Snežana and Avramović, Nataša and Gavrilović, Aleksandra and Jovanović, Silvana and Gođevac, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Tasic, Ljubica and Mandić, Boris",
year = "2023",
abstract = "Bipolar disorder (BD) is a brain disorder that causes changes in a person’s mood, energy, and ability to function. It has a prevalence of 60 million people worldwide, and it is among the top 20 diseases with the highest global burden. The complexity of this disease, including diverse genetic, environmental, and biochemical factors, and diagnoses based on the subjective recognition of symptoms without any clinical test of biomarker identification create significant difficulties in understanding and diagnosing BD. A 1H-NMR-based metabolomic study applying chemometrics of serum samples of Serbian patients with BD (33) and healthy controls (39) was explored, providing the identification of 22 metabolites for this disease. A biomarker set including threonine, aspartate, gamma-aminobutyric acid, 2-hydroxybutyric acid, serine, and mannose was established for the first time in BD serum samples by an NMR-based metabolomics study. Six identified metabolites (3-hydroxybutyric acid, arginine, lysine, tyrosine, phenylalanine, and glycerol) are in agreement with the previously determined NMR-based sets of serum biomarkers in Brazilian and/or Chinese patient samples. The same established metabolites (lactate, alanine, valine, leucine, isoleucine, glutamine, glutamate, glucose, and choline) in three different ethnic and geographic origins (Serbia, Brazil, and China) might have a crucial role in the realization of a universal set of NMR biomarkers for BD.",
publisher = "MDPI AG",
journal = "Metabolites",
title = "Metabolomic Profiling of Bipolar Disorder by 1H-NMR in Serbian Patients",
volume = "13",
number = "5",
pages = "607",
doi = "10.3390/metabo13050607"
}

Simić, K., Miladinović, Z., Todorović, N., Trifunović, S., Avramović, N., Gavrilović, A., Jovanović, S., Gođevac, D., Vujisić, L., Tešević, V., Tasic, L.,& Mandić, B.. (2023). Metabolomic Profiling of Bipolar Disorder by 1H-NMR in Serbian Patients. in Metabolites
MDPI AG., 13(5), 607.
https://doi.org/10.3390/metabo13050607

Simić K, Miladinović Z, Todorović N, Trifunović S, Avramović N, Gavrilović A, Jovanović S, Gođevac D, Vujisić L, Tešević V, Tasic L, Mandić B. Metabolomic Profiling of Bipolar Disorder by 1H-NMR in Serbian Patients. in Metabolites. 2023;13(5):607.
doi:10.3390/metabo13050607 .

Simić, Katarina, Miladinović, Zoran, Todorović, Nina, Trifunović, Snežana, Avramović, Nataša, Gavrilović, Aleksandra, Jovanović, Silvana, Gođevac, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Tasic, Ljubica, Mandić, Boris, "Metabolomic Profiling of Bipolar Disorder by 1H-NMR in Serbian Patients" in Metabolites, 13, no. 5 (2023):607,
https://doi.org/10.3390/metabo13050607 . .

TY  - CONF
AU  - Avramović, Nataša
AU  - Simić, Katarina
AU  - Miladinović, Zoran
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Gođevac, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Tasić, Ljubica
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7390
AB  - Bipolar disorder (BD) is a mental disorder that causes alteration of mood states including mania, depression, and euthymia and it is ranked as one of the leading causes of disability and premature mortality, with a prevalence of 60 million people worldwide. BD is a heterogenous illness including diverse genetic, environmental, and biochemical factors and its pathophysiology is still largely unknown. Diagnosis of BD exclusively depends on the subjective recognition of symptoms without any objective methods such as a clinical test of biomarker identification, instigating misdiagnosis, inadequate treatments and deficient clinical outcomes. 1H-NMR-based serum metabolomics of Serbian patients with BD (33) and healthy controls (39) contributed to identification of 22 metabolites for this disease. Threonine, aspartate, gamma-aminobutyric acid, 2-hydroxybutyric acid, serine, and mannose make a unique biomarker set, and were confirmed for the first time in BD Serbian serum samples. Additional six identified metabolites (3-hydroxybutyric acid, arginine, lysine, tyrosine, phenylalanine, and glycerol) are in accordance with the previously determined NMR-based sets of serum BD biomarkers in Brazilian and/or Chinese patient samples, while nine identified metabolites (lactate, alanine, valine, leucine, isoleucine, glutamine, glutamate, glucose, and choline) are the same established biomarkers in three different ethnic and geographic origins (Serbia, Brazil, and China). The same confirmed metabolites are an indicator of the right path in discovery of the universal set of BD biomarkers by NMR.
PB  - International Association of Physical Chemists
C3  - Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia
T1  - 1H-NMR-based serum metabolomics of bipolar disorder patients
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_cer_7390
ER  - 

@conference{
author = "Avramović, Nataša and Simić, Katarina and Miladinović, Zoran and Todorović, Nina and Trifunović, Snežana and Gavrilović, Aleksandra and Jovanović, Silvana and Gođevac, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Tasić, Ljubica and Mandić, Boris",
year = "2023",
abstract = "Bipolar disorder (BD) is a mental disorder that causes alteration of mood states including mania, depression, and euthymia and it is ranked as one of the leading causes of disability and premature mortality, with a prevalence of 60 million people worldwide. BD is a heterogenous illness including diverse genetic, environmental, and biochemical factors and its pathophysiology is still largely unknown. Diagnosis of BD exclusively depends on the subjective recognition of symptoms without any objective methods such as a clinical test of biomarker identification, instigating misdiagnosis, inadequate treatments and deficient clinical outcomes. 1H-NMR-based serum metabolomics of Serbian patients with BD (33) and healthy controls (39) contributed to identification of 22 metabolites for this disease. Threonine, aspartate, gamma-aminobutyric acid, 2-hydroxybutyric acid, serine, and mannose make a unique biomarker set, and were confirmed for the first time in BD Serbian serum samples. Additional six identified metabolites (3-hydroxybutyric acid, arginine, lysine, tyrosine, phenylalanine, and glycerol) are in accordance with the previously determined NMR-based sets of serum BD biomarkers in Brazilian and/or Chinese patient samples, while nine identified metabolites (lactate, alanine, valine, leucine, isoleucine, glutamine, glutamate, glucose, and choline) are the same established biomarkers in three different ethnic and geographic origins (Serbia, Brazil, and China). The same confirmed metabolites are an indicator of the right path in discovery of the universal set of BD biomarkers by NMR.",
publisher = "International Association of Physical Chemists",
journal = "Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia",
title = "1H-NMR-based serum metabolomics of bipolar disorder patients",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_cer_7390"
}

Avramović, N., Simić, K., Miladinović, Z., Todorović, N., Trifunović, S., Gavrilović, A., Jovanović, S., Gođevac, D., Vujisić, L., Tešević, V., Tasić, L.,& Mandić, B.. (2023). 1H-NMR-based serum metabolomics of bipolar disorder patients. in Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia
International Association of Physical Chemists., 67-67.
https://hdl.handle.net/21.15107/rcub_cer_7390

Avramović N, Simić K, Miladinović Z, Todorović N, Trifunović S, Gavrilović A, Jovanović S, Gođevac D, Vujisić L, Tešević V, Tasić L, Mandić B. 1H-NMR-based serum metabolomics of bipolar disorder patients. in Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia. 2023;:67-67.
https://hdl.handle.net/21.15107/rcub_cer_7390 .

Avramović, Nataša, Simić, Katarina, Miladinović, Zoran, Todorović, Nina, Trifunović, Snežana, Gavrilović, Aleksandra, Jovanović, Silvana, Gođevac, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Tasić, Ljubica, Mandić, Boris, "1H-NMR-based serum metabolomics of bipolar disorder patients" in Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia (2023):67-67,
https://hdl.handle.net/21.15107/rcub_cer_7390 .

TY  - CONF
AU  - Avramović, Nataša
AU  - Simić, Katarina
AU  - Miladinović, Zoran
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Gođevac, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Tasić, Ljubica
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7391
AB  - Bipolar disorder (BD) is a mental disorder that causes alteration of mood states including mania, depression, and euthymia and it is ranked as one of the leading causes of disability and premature mortality, with a prevalence of 60 million people worldwide. BD is a heterogenous illness including diverse genetic, environmental, and biochemical factors and its pathophysiology is still largely unknown. Diagnosis of BD exclusively depends on the subjective recognition of symptoms without any objective methods such as a clinical test of biomarker identification, instigating misdiagnosis, inadequate treatments and deficient clinical outcomes. 1H-NMR-based serum metabolomics of Serbian patients with BD (33) and healthy controls (39) contributed to identification of 22 metabolites for this disease. Threonine, aspartate, gamma-aminobutyric acid, 2-hydroxybutyric acid, serine, and mannose make a unique biomarker set, and were confirmed for the first time in BD Serbian serum samples. Additional six identified metabolites (3-hydroxybutyric acid, arginine, lysine, tyrosine, phenylalanine, and glycerol) are in accordance with the previously determined NMR-based sets of serum BD biomarkers in Brazilian and/or Chinese patient samples, while nine identified metabolites (lactate, alanine, valine, leucine, isoleucine, glutamine, glutamate, glucose, and choline) are the same established biomarkers in three different ethnic and geographic origins (Serbia, Brazil, and China). The same confirmed metabolites are an indicator of the right path in discovery of the universal set of BD biomarkers by NMR.
T1  - Poster presentation: 1H-NMR-based serum metabolomics of bipolar disorder patients
IS  - P26
UR  - https://hdl.handle.net/21.15107/rcub_cer_7391
ER  - 

@conference{
author = "Avramović, Nataša and Simić, Katarina and Miladinović, Zoran and Todorović, Nina and Trifunović, Snežana and Gavrilović, Aleksandra and Jovanović, Silvana and Gođevac, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Tasić, Ljubica and Mandić, Boris",
year = "2023",
abstract = "Bipolar disorder (BD) is a mental disorder that causes alteration of mood states including mania, depression, and euthymia and it is ranked as one of the leading causes of disability and premature mortality, with a prevalence of 60 million people worldwide. BD is a heterogenous illness including diverse genetic, environmental, and biochemical factors and its pathophysiology is still largely unknown. Diagnosis of BD exclusively depends on the subjective recognition of symptoms without any objective methods such as a clinical test of biomarker identification, instigating misdiagnosis, inadequate treatments and deficient clinical outcomes. 1H-NMR-based serum metabolomics of Serbian patients with BD (33) and healthy controls (39) contributed to identification of 22 metabolites for this disease. Threonine, aspartate, gamma-aminobutyric acid, 2-hydroxybutyric acid, serine, and mannose make a unique biomarker set, and were confirmed for the first time in BD Serbian serum samples. Additional six identified metabolites (3-hydroxybutyric acid, arginine, lysine, tyrosine, phenylalanine, and glycerol) are in accordance with the previously determined NMR-based sets of serum BD biomarkers in Brazilian and/or Chinese patient samples, while nine identified metabolites (lactate, alanine, valine, leucine, isoleucine, glutamine, glutamate, glucose, and choline) are the same established biomarkers in three different ethnic and geographic origins (Serbia, Brazil, and China). The same confirmed metabolites are an indicator of the right path in discovery of the universal set of BD biomarkers by NMR.",
title = "Poster presentation: 1H-NMR-based serum metabolomics of bipolar disorder patients",
number = "P26",
url = "https://hdl.handle.net/21.15107/rcub_cer_7391"
}

Avramović, N., Simić, K., Miladinović, Z., Todorović, N., Trifunović, S., Gavrilović, A., Jovanović, S., Gođevac, D., Vujisić, L., Tešević, V., Tasić, L.,& Mandić, B.. (2023). Poster presentation: 1H-NMR-based serum metabolomics of bipolar disorder patients. (P26).
https://hdl.handle.net/21.15107/rcub_cer_7391

Avramović N, Simić K, Miladinović Z, Todorović N, Trifunović S, Gavrilović A, Jovanović S, Gođevac D, Vujisić L, Tešević V, Tasić L, Mandić B. Poster presentation: 1H-NMR-based serum metabolomics of bipolar disorder patients. 2023;(P26).
https://hdl.handle.net/21.15107/rcub_cer_7391 .

Avramović, Nataša, Simić, Katarina, Miladinović, Zoran, Todorović, Nina, Trifunović, Snežana, Gavrilović, Aleksandra, Jovanović, Silvana, Gođevac, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Tasić, Ljubica, Mandić, Boris, "Poster presentation: 1H-NMR-based serum metabolomics of bipolar disorder patients", no. P26 (2023),
https://hdl.handle.net/21.15107/rcub_cer_7391 .

TY  - CONF
AU  - Avramović, Nataša
AU  - Simić, Katarina
AU  - Miladinović, Zoran
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Gođevac, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Tasić, Ljubica
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7392
AB  - Schizophrenia (SCZ) is a brain disease leading to significant functional impairments and premature death, and it affects 20 million people worldwide. Due to the complexity of this disease including different genetic and environmental factors, there is a lack in understanding pathophysiology and diagnosis of schizophrenia. In order to overcome existing gaps, the establishment of a universal set of SCZ biomarkers has a crucial role. Metabonomic study of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39) by 1H-NMR analyses associated with chemometrics, provided the identification of 26 metabolites/biomarkers for this disorder. The biomarker set including aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols was established for the first-time in SCZ serum samples of Serbian patients by 1H-NMR experiments. The other 22 identified metabolites are in agreement with the previously confirmed NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. The same 13 metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) were established in all SCZ samples from three countries of different ethnicity and geographical origins (Serbia, Brazil and China). These results emphasize the crucial role in the possibility of their application as biomarkers for diagnosis of SCZ, reliable monitoring of treatment response and clinical outcomes.
PB  - International Association of Physical Chemists
C3  - Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia
T1  - 1H-NMR metabonomic view on schizophrenia
SP  - 68
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_cer_7392
ER  - 

@conference{
author = "Avramović, Nataša and Simić, Katarina and Miladinović, Zoran and Todorović, Nina and Trifunović, Snežana and Gavrilović, Aleksandra and Jovanović, Silvana and Gođevac, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Tasić, Ljubica and Mandić, Boris",
year = "2023",
abstract = "Schizophrenia (SCZ) is a brain disease leading to significant functional impairments and premature death, and it affects 20 million people worldwide. Due to the complexity of this disease including different genetic and environmental factors, there is a lack in understanding pathophysiology and diagnosis of schizophrenia. In order to overcome existing gaps, the establishment of a universal set of SCZ biomarkers has a crucial role. Metabonomic study of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39) by 1H-NMR analyses associated with chemometrics, provided the identification of 26 metabolites/biomarkers for this disorder. The biomarker set including aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols was established for the first-time in SCZ serum samples of Serbian patients by 1H-NMR experiments. The other 22 identified metabolites are in agreement with the previously confirmed NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. The same 13 metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) were established in all SCZ samples from three countries of different ethnicity and geographical origins (Serbia, Brazil and China). These results emphasize the crucial role in the possibility of their application as biomarkers for diagnosis of SCZ, reliable monitoring of treatment response and clinical outcomes.",
publisher = "International Association of Physical Chemists",
journal = "Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia",
title = "1H-NMR metabonomic view on schizophrenia",
pages = "68-68",
url = "https://hdl.handle.net/21.15107/rcub_cer_7392"
}

Avramović, N., Simić, K., Miladinović, Z., Todorović, N., Trifunović, S., Gavrilović, A., Jovanović, S., Gođevac, D., Vujisić, L., Tešević, V., Tasić, L.,& Mandić, B.. (2023). 1H-NMR metabonomic view on schizophrenia. in Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia
International Association of Physical Chemists., 68-68.
https://hdl.handle.net/21.15107/rcub_cer_7392

Avramović N, Simić K, Miladinović Z, Todorović N, Trifunović S, Gavrilović A, Jovanović S, Gođevac D, Vujisić L, Tešević V, Tasić L, Mandić B. 1H-NMR metabonomic view on schizophrenia. in Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia. 2023;:68-68.
https://hdl.handle.net/21.15107/rcub_cer_7392 .

Avramović, Nataša, Simić, Katarina, Miladinović, Zoran, Todorović, Nina, Trifunović, Snežana, Gavrilović, Aleksandra, Jovanović, Silvana, Gođevac, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Tasić, Ljubica, Mandić, Boris, "1H-NMR metabonomic view on schizophrenia" in Book of Abstracts, 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK, September 4-6, 2023, Belgrade, Serbia (2023):68-68,
https://hdl.handle.net/21.15107/rcub_cer_7392 .

TY  - CONF
AU  - Avramović, Nataša
AU  - Simić, Katarina
AU  - Miladinović, Zoran
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Gođevac, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Tasić, Ljubica
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7393
AB  - Schizophrenia (SCZ) is a brain disease leading to significant functional impairments and premature death, and it affects 20 million people worldwide. Due to the complexity of this disease including different genetic and environmental factors, there is a lack in understanding pathophysiology and diagnosis of schizophrenia. In order to overcome existing gaps, the establishment of a universal set of SCZ biomarkers has a crucial role. Metabonomic study of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39) by 1H-NMR analyses associated with chemometrics, provided the identification of 26 metabolites/biomarkers for this disorder. The biomarker set including aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols was established for the first-time in SCZ serum samples of Serbian patients by 1H-NMR experiments. The other 22 identified metabolites are in agreement with the previously confirmed NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. The same 13 metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) were established in all SCZ samples from three countries of different ethnicity and geographical origins (Serbia, Brazil and China). These results emphasize the crucial role in the possibility of their application as biomarkers for diagnosis of SCZ, reliable monitoring of treatment response and clinical outcomes.
T1  - Poster presentation: 1H-NMR metabonomic view on schizophrenia
IS  - P27
UR  - https://hdl.handle.net/21.15107/rcub_cer_7393
ER  - 

@conference{
author = "Avramović, Nataša and Simić, Katarina and Miladinović, Zoran and Todorović, Nina and Trifunović, Snežana and Gavrilović, Aleksandra and Jovanović, Silvana and Gođevac, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Tasić, Ljubica and Mandić, Boris",
year = "2023",
abstract = "Schizophrenia (SCZ) is a brain disease leading to significant functional impairments and premature death, and it affects 20 million people worldwide. Due to the complexity of this disease including different genetic and environmental factors, there is a lack in understanding pathophysiology and diagnosis of schizophrenia. In order to overcome existing gaps, the establishment of a universal set of SCZ biomarkers has a crucial role. Metabonomic study of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39) by 1H-NMR analyses associated with chemometrics, provided the identification of 26 metabolites/biomarkers for this disorder. The biomarker set including aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols was established for the first-time in SCZ serum samples of Serbian patients by 1H-NMR experiments. The other 22 identified metabolites are in agreement with the previously confirmed NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. The same 13 metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) were established in all SCZ samples from three countries of different ethnicity and geographical origins (Serbia, Brazil and China). These results emphasize the crucial role in the possibility of their application as biomarkers for diagnosis of SCZ, reliable monitoring of treatment response and clinical outcomes.",
title = "Poster presentation: 1H-NMR metabonomic view on schizophrenia",
number = "P27",
url = "https://hdl.handle.net/21.15107/rcub_cer_7393"
}

Avramović, N., Simić, K., Miladinović, Z., Todorović, N., Trifunović, S., Gavrilović, A., Jovanović, S., Gođevac, D., Vujisić, L., Tešević, V., Tasić, L.,& Mandić, B.. (2023). Poster presentation: 1H-NMR metabonomic view on schizophrenia. (P27).
https://hdl.handle.net/21.15107/rcub_cer_7393

Avramović N, Simić K, Miladinović Z, Todorović N, Trifunović S, Gavrilović A, Jovanović S, Gođevac D, Vujisić L, Tešević V, Tasić L, Mandić B. Poster presentation: 1H-NMR metabonomic view on schizophrenia. 2023;(P27).
https://hdl.handle.net/21.15107/rcub_cer_7393 .

Avramović, Nataša, Simić, Katarina, Miladinović, Zoran, Todorović, Nina, Trifunović, Snežana, Gavrilović, Aleksandra, Jovanović, Silvana, Gođevac, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Tasić, Ljubica, Mandić, Boris, "Poster presentation: 1H-NMR metabonomic view on schizophrenia", no. P27 (2023),
https://hdl.handle.net/21.15107/rcub_cer_7393 .

TY  - JOUR
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Trifunović, Sara
AU  - Krstić-Ristivojević, Maja
AU  - Aćimović, Milica G.
AU  - Stanković Jeremić, Jovana
AU  - Lončar, Biljana
AU  - Tešević, Vele
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7198
AB  - As byproducts of essential oil distillation, hydrolates are used in natural cosmetics/biomedicine due to their beneficial skin effects. However, data on their safety with relevant biological targets, such as human skin cells, are scarce. Therefore, we have tested nine hydrolates from the Lamiaceae family with skin fibroblasts that are responsible for extracellular collagenous matrix builds. Thyme, oregano, and winter savoury hydrolates showed several times higher total phenolics, which correlated strongly with their radical scavenging and antioxidative capacity; there was no correlation between their viability profiles and the reducing sugar levels. No proteins/peptides were detected. All hydrolates appeared safe for prolonged skin exposure except for 10-fold diluted lavender, which showed cytotoxicity (~20%), as well as rosemary and lavandin (~10%) using viability, DNA synthesis, and cell count testing. Clary sage, oregano, lemon balm, and thyme hydrolates (10-fold diluted) increased fibroblast viability and/or proliferation by 10–30% compared with the control, while their viability remained unaffected by Mentha and winter savoury. In line with the STITCH database, increased viability could be attributed to thymol presence in oregano and thyme hydrolates in lemon balm, which is most likely attributable to neral and geranial. The proliferative effect of clary sage could be supported by alpha-terpineol, not linalool. The major volatile organic compounds (VOCs) associated with cytotoxic effects on fibroblasts were borneol, 1,8-cineole, and terpinene-4-ol. Further research with pure compounds is warranted to confirm the roles of VOCs in the observed effects that are relevant to cosmetic and wound healing aspects.
PB  - MDPI
T2  - Antioxidants
T1  - Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts
VL  - 12
IS  - 11
DO  - 10.3390/antiox12111988
ER  - 

@article{
author = "Smiljanić, Katarina and Prodić, Ivana and Trifunović, Sara and Krstić-Ristivojević, Maja and Aćimović, Milica G. and Stanković Jeremić, Jovana and Lončar, Biljana and Tešević, Vele",
year = "2023",
abstract = "As byproducts of essential oil distillation, hydrolates are used in natural cosmetics/biomedicine due to their beneficial skin effects. However, data on their safety with relevant biological targets, such as human skin cells, are scarce. Therefore, we have tested nine hydrolates from the Lamiaceae family with skin fibroblasts that are responsible for extracellular collagenous matrix builds. Thyme, oregano, and winter savoury hydrolates showed several times higher total phenolics, which correlated strongly with their radical scavenging and antioxidative capacity; there was no correlation between their viability profiles and the reducing sugar levels. No proteins/peptides were detected. All hydrolates appeared safe for prolonged skin exposure except for 10-fold diluted lavender, which showed cytotoxicity (~20%), as well as rosemary and lavandin (~10%) using viability, DNA synthesis, and cell count testing. Clary sage, oregano, lemon balm, and thyme hydrolates (10-fold diluted) increased fibroblast viability and/or proliferation by 10–30% compared with the control, while their viability remained unaffected by Mentha and winter savoury. In line with the STITCH database, increased viability could be attributed to thymol presence in oregano and thyme hydrolates in lemon balm, which is most likely attributable to neral and geranial. The proliferative effect of clary sage could be supported by alpha-terpineol, not linalool. The major volatile organic compounds (VOCs) associated with cytotoxic effects on fibroblasts were borneol, 1,8-cineole, and terpinene-4-ol. Further research with pure compounds is warranted to confirm the roles of VOCs in the observed effects that are relevant to cosmetic and wound healing aspects.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts",
volume = "12",
number = "11",
doi = "10.3390/antiox12111988"
}

Smiljanić, K., Prodić, I., Trifunović, S., Krstić-Ristivojević, M., Aćimović, M. G., Stanković Jeremić, J., Lončar, B.,& Tešević, V.. (2023). Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts. in Antioxidants
MDPI., 12(11).
https://doi.org/10.3390/antiox12111988

Smiljanić K, Prodić I, Trifunović S, Krstić-Ristivojević M, Aćimović MG, Stanković Jeremić J, Lončar B, Tešević V. Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts. in Antioxidants. 2023;12(11).
doi:10.3390/antiox12111988 .

Smiljanić, Katarina, Prodić, Ivana, Trifunović, Sara, Krstić-Ristivojević, Maja, Aćimović, Milica G., Stanković Jeremić, Jovana, Lončar, Biljana, Tešević, Vele, "Multistep Approach Points to Compounds Responsible for the Biological Activity and Safety of Hydrolates from Nine Lamiaceae Medicinal Plants on Human Skin Fibroblasts" in Antioxidants, 12, no. 11 (2023),
https://doi.org/10.3390/antiox12111988 . .

TY  - JOUR
AU  - Jadranin, Milka
AU  - Avramović, Nataša
AU  - Miladinović, Zoran P.
AU  - Gavrilović, Aleksandra
AU  - Tasic, Ljubica
AU  - Tešević, Vele
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7180
AB  - The Lipidomic profiles of serum samples from patients with bipolar disorder (BD) and healthy controls (C) were explored and compared. The sample cohort included 31 BD patients and 31 control individuals. An untargeted lipidomics study applying liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) was conducted to achieve the lipid profiles. Multivariate statistical analyses (principal component analysis and partial least squares discriminant analysis) were performed, and fifty-six differential lipids were confirmed in BD and controls. Our results pointed to alterations in lipid metabolism, including pathways of glycerophospholipids, sphingolipids, glycerolipids, and sterol lipids, in BD patient sera. This study emphasized the role of lipid pathways in BD, and comprehensive research using the LC-HRMS platform is necessary for future application in the diagnosis and improvement of BD treatments.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia
VL  - 24
IS  - 22
SP  - 16025
DO  - 10.3390/ijms242216025
ER  - 

@article{
author = "Jadranin, Milka and Avramović, Nataša and Miladinović, Zoran P. and Gavrilović, Aleksandra and Tasic, Ljubica and Tešević, Vele and Mandić, Boris",
year = "2023",
abstract = "The Lipidomic profiles of serum samples from patients with bipolar disorder (BD) and healthy controls (C) were explored and compared. The sample cohort included 31 BD patients and 31 control individuals. An untargeted lipidomics study applying liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) was conducted to achieve the lipid profiles. Multivariate statistical analyses (principal component analysis and partial least squares discriminant analysis) were performed, and fifty-six differential lipids were confirmed in BD and controls. Our results pointed to alterations in lipid metabolism, including pathways of glycerophospholipids, sphingolipids, glycerolipids, and sterol lipids, in BD patient sera. This study emphasized the role of lipid pathways in BD, and comprehensive research using the LC-HRMS platform is necessary for future application in the diagnosis and improvement of BD treatments.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia",
volume = "24",
number = "22",
pages = "16025",
doi = "10.3390/ijms242216025"
}

Jadranin, M., Avramović, N., Miladinović, Z. P., Gavrilović, A., Tasic, L., Tešević, V.,& Mandić, B.. (2023). Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia. in International Journal of Molecular Sciences
MDPI., 24(22), 16025.
https://doi.org/10.3390/ijms242216025

Jadranin M, Avramović N, Miladinović ZP, Gavrilović A, Tasic L, Tešević V, Mandić B. Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia. in International Journal of Molecular Sciences. 2023;24(22):16025.
doi:10.3390/ijms242216025 .

Jadranin, Milka, Avramović, Nataša, Miladinović, Zoran P., Gavrilović, Aleksandra, Tasic, Ljubica, Tešević, Vele, Mandić, Boris, "Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia" in International Journal of Molecular Sciences, 24, no. 22 (2023):16025,
https://doi.org/10.3390/ijms242216025 . .

TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Krstić, Gordana B.
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7540
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes’ chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
VL  - 12
IS  - 24
SP  - 4181
DO  - 10.3390/plants12244181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan and Krstić, Gordana B.",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes’ chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
volume = "12",
number = "24",
pages = "4181",
doi = "10.3390/plants12244181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S.,& Krstić, G. B.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević S, Krstić GB. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, Krstić, Gordana B., "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Todorović, Nina
AU  - Jadranin, Milka
AU  - Đorđević, Iris
AU  - Milosavljević, Slobodan
AU  - Mandić, Boris
AU  - Tešević, Vele
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7191
AB  - A new auronolignan, named cotinignan B, was isolated from Cotinus coggygria Scop. Structure elucidation was performed on the basis of 1H, 13C NMR, COSY, NOESY, HSQC, and HMBC experiments, supported with HR-ESI-MS, IR and UV. Additional J-HMBC NMR experiment was essential to resolve the configuration of the trisubstituted double bond. This compound represents the secondly discovered natural compound belonging to the rare auronolignan type.
PB  - Springer
T2  - Chemistry of Natural Compounds
T1  - A New Auronolignan from the Cotinus coggygria Heartwood
VL  - 59
IS  - 3
SP  - 428
EP  - 430
DO  - 10.1007/s10600-023-04016-5
ER  - 

@article{
author = "Novaković, Miroslav and Todorović, Nina and Jadranin, Milka and Đorđević, Iris and Milosavljević, Slobodan and Mandić, Boris and Tešević, Vele",
year = "2023",
abstract = "A new auronolignan, named cotinignan B, was isolated from Cotinus coggygria Scop. Structure elucidation was performed on the basis of 1H, 13C NMR, COSY, NOESY, HSQC, and HMBC experiments, supported with HR-ESI-MS, IR and UV. Additional J-HMBC NMR experiment was essential to resolve the configuration of the trisubstituted double bond. This compound represents the secondly discovered natural compound belonging to the rare auronolignan type.",
publisher = "Springer",
journal = "Chemistry of Natural Compounds",
title = "A New Auronolignan from the Cotinus coggygria Heartwood",
volume = "59",
number = "3",
pages = "428-430",
doi = "10.1007/s10600-023-04016-5"
}

Novaković, M., Todorović, N., Jadranin, M., Đorđević, I., Milosavljević, S., Mandić, B.,& Tešević, V.. (2023). A New Auronolignan from the Cotinus coggygria Heartwood. in Chemistry of Natural Compounds
Springer., 59(3), 428-430.
https://doi.org/10.1007/s10600-023-04016-5

Novaković M, Todorović N, Jadranin M, Đorđević I, Milosavljević S, Mandić B, Tešević V. A New Auronolignan from the Cotinus coggygria Heartwood. in Chemistry of Natural Compounds. 2023;59(3):428-430.
doi:10.1007/s10600-023-04016-5 .

Novaković, Miroslav, Todorović, Nina, Jadranin, Milka, Đorđević, Iris, Milosavljević, Slobodan, Mandić, Boris, Tešević, Vele, "A New Auronolignan from the Cotinus coggygria Heartwood" in Chemistry of Natural Compounds, 59, no. 3 (2023):428-430,
https://doi.org/10.1007/s10600-023-04016-5 . .

TY  - JOUR
AU  - Nikolić, Biljana M.
AU  - Ballian, Dalibor A.
AU  - Đorđević, Iris
AU  - Rajčević, Nemanja
AU  - Todosijević, Marina M.
AU  - Stanković Jeremić, Jovana
AU  - Mitić, Zorica S.
AU  - Bojović, Srđan
AU  - Tešević, Vele
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6680
AB  - Population variability of n-alkanes in the cuticular waxes of Serbian spruce needles from Bosnia and Herzegovina was investigated for the first time. n-Alkanes exhibited composition variability ranging from C18 to C35. The divergence of populations from Bosnia and Herzegovina and previously investigated Serbian populations was statistically confirmed by PCA, DA, and CA (Cluster analysis), which could support the fact that there are two possible spruce chemotypes: one relating to the Bosnian-Herzegovinian and the other to the Serbian populations. Climate parameters (precipitation, temperature, wind, and solar radiation) approved this conclusion.
PB  - Elsevier Ltd.
T2  - Biochemical Systematics and Ecology
T1  - n-Alkanes variability in natural populations of Picea omorika (Pančić) Purk. From Bosnia and Herzegovina
VL  - 106
SP  - 104544
DO  - 10.1016/j.bse.2022.104544
ER  - 

@article{
author = "Nikolić, Biljana M. and Ballian, Dalibor A. and Đorđević, Iris and Rajčević, Nemanja and Todosijević, Marina M. and Stanković Jeremić, Jovana and Mitić, Zorica S. and Bojović, Srđan and Tešević, Vele",
year = "2023",
abstract = "Population variability of n-alkanes in the cuticular waxes of Serbian spruce needles from Bosnia and Herzegovina was investigated for the first time. n-Alkanes exhibited composition variability ranging from C18 to C35. The divergence of populations from Bosnia and Herzegovina and previously investigated Serbian populations was statistically confirmed by PCA, DA, and CA (Cluster analysis), which could support the fact that there are two possible spruce chemotypes: one relating to the Bosnian-Herzegovinian and the other to the Serbian populations. Climate parameters (precipitation, temperature, wind, and solar radiation) approved this conclusion.",
publisher = "Elsevier Ltd.",
journal = "Biochemical Systematics and Ecology",
title = "n-Alkanes variability in natural populations of Picea omorika (Pančić) Purk. From Bosnia and Herzegovina",
volume = "106",
pages = "104544",
doi = "10.1016/j.bse.2022.104544"
}

Nikolić, B. M., Ballian, D. A., Đorđević, I., Rajčević, N., Todosijević, M. M., Stanković Jeremić, J., Mitić, Z. S., Bojović, S.,& Tešević, V.. (2023). n-Alkanes variability in natural populations of Picea omorika (Pančić) Purk. From Bosnia and Herzegovina. in Biochemical Systematics and Ecology
Elsevier Ltd.., 106, 104544.
https://doi.org/10.1016/j.bse.2022.104544

Nikolić BM, Ballian DA, Đorđević I, Rajčević N, Todosijević MM, Stanković Jeremić J, Mitić ZS, Bojović S, Tešević V. n-Alkanes variability in natural populations of Picea omorika (Pančić) Purk. From Bosnia and Herzegovina. in Biochemical Systematics and Ecology. 2023;106:104544.
doi:10.1016/j.bse.2022.104544 .

Nikolić, Biljana M., Ballian, Dalibor A., Đorđević, Iris, Rajčević, Nemanja, Todosijević, Marina M., Stanković Jeremić, Jovana, Mitić, Zorica S., Bojović, Srđan, Tešević, Vele, "n-Alkanes variability in natural populations of Picea omorika (Pančić) Purk. From Bosnia and Herzegovina" in Biochemical Systematics and Ecology, 106 (2023):104544,
https://doi.org/10.1016/j.bse.2022.104544 . .

TY  - JOUR
AU  - Paunović, Dusanka
AU  - Mirković, Milica
AU  - Mirković, Nemanja
AU  - Tešević, Vele
AU  - Stanković Jeremić, Jovana
AU  - Todosijević, Marina
AU  - Radulović, Zorica
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7226
AB  - The use of truffles in food is based mainly on the addition of artificial flavors, aiming to achieve an intense aroma in the products. As truffle is a natural product with nutritional and functional properties, it is important to find an optimal method for truffle storage. As the microbiota contribute to truffle aroma, the bacterial and yeast compositions in the rhizosphere and fruiting body of the truffle and the impact of different freezing methods on the volatile profile of the truffle Tuber aestivum during 90 days of the storage were determined. Bacteria and yeasts isolates were identified using 16s rRNA and 18s rRNA. The effect of freezing truffles at -20°C and -80°C with and without previous dipping in liquid N2 on the volatile compounds was observed using GC/MS. The results demonstrated that the isolated bacteria belonged to the phylum Proteobacteria, Firmicutes and Actinobacteria, and the identified species mainly belonged to Firmicutes, genus Bacillus sp. Isolated yeasts were identified as Cryptococcus sp., Debaromyces hanseinii, Candida fermentati and Rhodotorula mucilaginosa. The GC/MS analysis revealed that frozen truffle samples were richer in the compounds 2-butanone, 2-methyl-butanal, methanethiol and 2-butanol after freezing or during storage. The content of DMS, acetaldehyde, 3-octanone, ethanol, and 2-methyl-1-propanol significantly decreased immediately after freezing. Overall, the gained results indicated that freezing of truffles as a preservation method had profound effects on the volatile compounds, while previous dipping in liquid N2 showed no significant impact on the volatile profile of truffle Tuber aestivum.
AB  - Primena tartufa u hrani se uglavnom zasniva na dodavanju veštačkih aroma, sa  ciljem postizanja intenzivne arome tartufa u proizvodima. S obzirom na to da su  tartufi proizvodi sa nutritivnim i funkcionalnim karakteristikama, važno je pronaći  optimalan način za skladištenje tartufa. Pošto mikrobiota doprinosi aromi tartufa,  sastav bakterija i kvasaca u rizosferi i plodonosnom telu srpskog tartufa, kao i uticaj  različitih metoda smrzavanja na isparljiva jedinjenja tartufa Tuber aestivum ispitivan  je tokom 90 dana skladištenja. Bakterije i kvasci su izolovani iz svežeg tartufa i  izolati su identifikovani korišćenjem 16s rRNK i 18s rRNK. Uticaj smrzavanja  tartufa na -20°C i -80°C sa i bez prethodnog potapanja u tečni N2 na sadržaj  isparljivih jedinjenja ispitivan je korišćenjem GC/MS. Rezultati ispitivanja su  pokazali da izolovane bakterije pripadaju carstvu Proteobacteria, Firmicutes i  Actinobacteria, pri čemu identifikovane vrste uglavnom pripadaju Firmicutes, rod  Bacillus sp. Izolovani kvasci su identifikovani kao Cryptococcus sp., Debaromyces  hanseinii, Candida fermentati i Rhodotorula mucilaginosa. Analiza GC/MS je  ukazala na razlike u profilu isparljivih jedinjenja svežeg i smrznutog tartufa.  Smrznuti uzorci su više sadržali komponente kao što su 2-butanon, 2-metil-butanal,  metanetiol i 2-butanol nakon smrzavanja ili tokom skladištenja. Sadržaj DMS,  acetaldehida, 3-octanona, etanola, 2-metil-1-propanola značajno se smanjio odmah  nakon smrzavanja. Dobijeni rezultati ukazuju da smrzavanje tartufa kao metoda  konzervacije ima značajan uticaj na isparljiva jedinjenja, pri čemu potapanje u tečni  N2 nije pokazalo značajan uticaj na isparljiva jedinjenja tartufa Tuber aestivum.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of Agricultural Sciences, Belgrade
T1  - Serbian black truffle Tuber aestivum: Microbiota and effects of different freezing regimes on volatile aroma compounds during storage
T1  - Srpski crni tartuf tuber aestivum: mikrobiota I uticaj različitih režima smrzavanja na isparljiiva aromatična jedinjenja tokom skladištenja
VL  - 68
IS  - 3
SP  - 329
EP  - 346
DO  - 10.2298/JAS2303329P
ER  - 

@article{
author = "Paunović, Dusanka and Mirković, Milica and Mirković, Nemanja and Tešević, Vele and Stanković Jeremić, Jovana and Todosijević, Marina and Radulović, Zorica",
year = "2023",
abstract = "The use of truffles in food is based mainly on the addition of artificial flavors, aiming to achieve an intense aroma in the products. As truffle is a natural product with nutritional and functional properties, it is important to find an optimal method for truffle storage. As the microbiota contribute to truffle aroma, the bacterial and yeast compositions in the rhizosphere and fruiting body of the truffle and the impact of different freezing methods on the volatile profile of the truffle Tuber aestivum during 90 days of the storage were determined. Bacteria and yeasts isolates were identified using 16s rRNA and 18s rRNA. The effect of freezing truffles at -20°C and -80°C with and without previous dipping in liquid N2 on the volatile compounds was observed using GC/MS. The results demonstrated that the isolated bacteria belonged to the phylum Proteobacteria, Firmicutes and Actinobacteria, and the identified species mainly belonged to Firmicutes, genus Bacillus sp. Isolated yeasts were identified as Cryptococcus sp., Debaromyces hanseinii, Candida fermentati and Rhodotorula mucilaginosa. The GC/MS analysis revealed that frozen truffle samples were richer in the compounds 2-butanone, 2-methyl-butanal, methanethiol and 2-butanol after freezing or during storage. The content of DMS, acetaldehyde, 3-octanone, ethanol, and 2-methyl-1-propanol significantly decreased immediately after freezing. Overall, the gained results indicated that freezing of truffles as a preservation method had profound effects on the volatile compounds, while previous dipping in liquid N2 showed no significant impact on the volatile profile of truffle Tuber aestivum., Primena tartufa u hrani se uglavnom zasniva na dodavanju veštačkih aroma, sa  ciljem postizanja intenzivne arome tartufa u proizvodima. S obzirom na to da su  tartufi proizvodi sa nutritivnim i funkcionalnim karakteristikama, važno je pronaći  optimalan način za skladištenje tartufa. Pošto mikrobiota doprinosi aromi tartufa,  sastav bakterija i kvasaca u rizosferi i plodonosnom telu srpskog tartufa, kao i uticaj  različitih metoda smrzavanja na isparljiva jedinjenja tartufa Tuber aestivum ispitivan  je tokom 90 dana skladištenja. Bakterije i kvasci su izolovani iz svežeg tartufa i  izolati su identifikovani korišćenjem 16s rRNK i 18s rRNK. Uticaj smrzavanja  tartufa na -20°C i -80°C sa i bez prethodnog potapanja u tečni N2 na sadržaj  isparljivih jedinjenja ispitivan je korišćenjem GC/MS. Rezultati ispitivanja su  pokazali da izolovane bakterije pripadaju carstvu Proteobacteria, Firmicutes i  Actinobacteria, pri čemu identifikovane vrste uglavnom pripadaju Firmicutes, rod  Bacillus sp. Izolovani kvasci su identifikovani kao Cryptococcus sp., Debaromyces  hanseinii, Candida fermentati i Rhodotorula mucilaginosa. Analiza GC/MS je  ukazala na razlike u profilu isparljivih jedinjenja svežeg i smrznutog tartufa.  Smrznuti uzorci su više sadržali komponente kao što su 2-butanon, 2-metil-butanal,  metanetiol i 2-butanol nakon smrzavanja ili tokom skladištenja. Sadržaj DMS,  acetaldehida, 3-octanona, etanola, 2-metil-1-propanola značajno se smanjio odmah  nakon smrzavanja. Dobijeni rezultati ukazuju da smrzavanje tartufa kao metoda  konzervacije ima značajan uticaj na isparljiva jedinjenja, pri čemu potapanje u tečni  N2 nije pokazalo značajan uticaj na isparljiva jedinjenja tartufa Tuber aestivum.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of Agricultural Sciences, Belgrade",
title = "Serbian black truffle Tuber aestivum: Microbiota and effects of different freezing regimes on volatile aroma compounds during storage, Srpski crni tartuf tuber aestivum: mikrobiota I uticaj različitih režima smrzavanja na isparljiiva aromatična jedinjenja tokom skladištenja",
volume = "68",
number = "3",
pages = "329-346",
doi = "10.2298/JAS2303329P"
}

Paunović, D., Mirković, M., Mirković, N., Tešević, V., Stanković Jeremić, J., Todosijević, M.,& Radulović, Z.. (2023). Serbian black truffle Tuber aestivum: Microbiota and effects of different freezing regimes on volatile aroma compounds during storage. in Journal of Agricultural Sciences, Belgrade
Belgrade : Serbian Chemical Society., 68(3), 329-346.
https://doi.org/10.2298/JAS2303329P

Paunović D, Mirković M, Mirković N, Tešević V, Stanković Jeremić J, Todosijević M, Radulović Z. Serbian black truffle Tuber aestivum: Microbiota and effects of different freezing regimes on volatile aroma compounds during storage. in Journal of Agricultural Sciences, Belgrade. 2023;68(3):329-346.
doi:10.2298/JAS2303329P .

Paunović, Dusanka, Mirković, Milica, Mirković, Nemanja, Tešević, Vele, Stanković Jeremić, Jovana, Todosijević, Marina, Radulović, Zorica, "Serbian black truffle Tuber aestivum: Microbiota and effects of different freezing regimes on volatile aroma compounds during storage" in Journal of Agricultural Sciences, Belgrade, 68, no. 3 (2023):329-346,
https://doi.org/10.2298/JAS2303329P . .

TY  - JOUR
AU  - Sofrenić, Ivana
AU  - Anđelković, Boban
AU  - Gođevac, Dejan
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Ljujić, Jovana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5655
AB  - Chemotaxonomy presents various challenges that need to be overcome in order to obtain valid and reliable results. Individual genetic and environmental variations can give a false picture and lead to wrong conclusions. Applying a holistic approach, based on multivariate data analysis, these challenges can be overcome. Thus, a metabolomics approach has to be optimized depending on the subject of research. We used 1H NMR-based metabolomics as a potential chemotaxonomic tool on the selected Euphorbia species growing wild in Serbia. Principal components analysis (PCA), soft independent modeling by class analogy (SIMCA) and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) were used to analyze obtained NMR data in order to reveal chemotaxonomic biomarkers. The standard protocol for plant metabolomics was optimized aiming to extract more specific metabolites, which are characteristic for the Euphorbia genus. The obtained models were validated, which revealed that variables unique for each species were associated with certain classes of molecules according to literature data. In E. salicifolia, acacetin-7-O-glycoside (not found before in the species) was detected, and the structure of the aglycone part was solved based on 2D NMR data. In the presented paper, we have shown that metabolomics can be successfully used in Euphorbia chemotaxonomy.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Plants
T1  - Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia
VL  - 12
IS  - 2
SP  - 262
DO  - 10.3390/plants12020262
ER  - 

@article{
author = "Sofrenić, Ivana and Anđelković, Boban and Gođevac, Dejan and Ivanović, Stefan and Simić, Katarina and Ljujić, Jovana and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "Chemotaxonomy presents various challenges that need to be overcome in order to obtain valid and reliable results. Individual genetic and environmental variations can give a false picture and lead to wrong conclusions. Applying a holistic approach, based on multivariate data analysis, these challenges can be overcome. Thus, a metabolomics approach has to be optimized depending on the subject of research. We used 1H NMR-based metabolomics as a potential chemotaxonomic tool on the selected Euphorbia species growing wild in Serbia. Principal components analysis (PCA), soft independent modeling by class analogy (SIMCA) and Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) were used to analyze obtained NMR data in order to reveal chemotaxonomic biomarkers. The standard protocol for plant metabolomics was optimized aiming to extract more specific metabolites, which are characteristic for the Euphorbia genus. The obtained models were validated, which revealed that variables unique for each species were associated with certain classes of molecules according to literature data. In E. salicifolia, acacetin-7-O-glycoside (not found before in the species) was detected, and the structure of the aglycone part was solved based on 2D NMR data. In the presented paper, we have shown that metabolomics can be successfully used in Euphorbia chemotaxonomy.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Plants",
title = "Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia",
volume = "12",
number = "2",
pages = "262",
doi = "10.3390/plants12020262"
}

Sofrenić, I., Anđelković, B., Gođevac, D., Ivanović, S., Simić, K., Ljujić, J., Tešević, V.,& Milosavljević, S.. (2023). Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia. in Plants
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 262.
https://doi.org/10.3390/plants12020262

Sofrenić I, Anđelković B, Gođevac D, Ivanović S, Simić K, Ljujić J, Tešević V, Milosavljević S. Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia. in Plants. 2023;12(2):262.
doi:10.3390/plants12020262 .

Sofrenić, Ivana, Anđelković, Boban, Gođevac, Dejan, Ivanović, Stefan, Simić, Katarina, Ljujić, Jovana, Tešević, Vele, Milosavljević, Slobodan, "Metabolomics as a Potential Chemotaxonomical Tool: Application on the Selected Euphorbia Species Growing Wild in Serbia" in Plants, 12, no. 2 (2023):262,
https://doi.org/10.3390/plants12020262 . .

TY  - CONF
AU  - Jadranin, Milka
AU  - Krstić, Gordana
AU  - Savić, Danica
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Lupšić, Ema
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7270
AB  - Euphorbia cyparissias L. (cypress spurge) is one of the many species of the genus Euphorbia. Like other plant species of the genus, E. cyparissias is a source of biologically active compounds [1, 2]. The aim of this research was to profile the chloroform-methanol latex extract and detect the type of secondary metabolites of Serbian E. cyparissias, collected in Deliblato Sand 
(Serbia) in May 2022, using liquid chromatography – electrospray ionisation mass spectrometry. Based on the obtained results, terpene derivatives have been found as the most abundant in the latex extract, mainly diterpenes (jatrophanes and ingenanes) and triterpenes. Also, the anticancer activ ity of the latex extract on the cell lines NCI-H460, NCI-H460/R, U87, U87/
TxR and MRC-5, as well as the ability to inhibit P-gp, were examined. Latex extract exhibited anticancer activity against all tested cell lines, and also 
proved to be a strong P-gp inhibitor. The obtained results are in accordance with the literature data because jatrophanes are well-known strong P-gp 
inhibitors [3]. These preliminary results indicate that this plant material is a rich source of biologically active compounds and detailed phytochemical research of E. cyparissias is further planned.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry
SP  - 184
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_cer_7270
ER  - 

@conference{
author = "Jadranin, Milka and Krstić, Gordana and Savić, Danica and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Pešić, Milica and Podolski-Renić, Ana and Lupšić, Ema and Milosavljević, Slobodan",
year = "2023",
abstract = "Euphorbia cyparissias L. (cypress spurge) is one of the many species of the genus Euphorbia. Like other plant species of the genus, E. cyparissias is a source of biologically active compounds [1, 2]. The aim of this research was to profile the chloroform-methanol latex extract and detect the type of secondary metabolites of Serbian E. cyparissias, collected in Deliblato Sand 
(Serbia) in May 2022, using liquid chromatography – electrospray ionisation mass spectrometry. Based on the obtained results, terpene derivatives have been found as the most abundant in the latex extract, mainly diterpenes (jatrophanes and ingenanes) and triterpenes. Also, the anticancer activ ity of the latex extract on the cell lines NCI-H460, NCI-H460/R, U87, U87/
TxR and MRC-5, as well as the ability to inhibit P-gp, were examined. Latex extract exhibited anticancer activity against all tested cell lines, and also 
proved to be a strong P-gp inhibitor. The obtained results are in accordance with the literature data because jatrophanes are well-known strong P-gp 
inhibitors [3]. These preliminary results indicate that this plant material is a rich source of biologically active compounds and detailed phytochemical research of E. cyparissias is further planned.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry",
pages = "184-185",
url = "https://hdl.handle.net/21.15107/rcub_cer_7270"
}

Jadranin, M., Krstić, G., Savić, D., Novaković, M., Lekić, S., Tešević, V., Pešić, M., Podolski-Renić, A., Lupšić, E.,& Milosavljević, S.. (2023). Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 184-185.
https://hdl.handle.net/21.15107/rcub_cer_7270

Jadranin M, Krstić G, Savić D, Novaković M, Lekić S, Tešević V, Pešić M, Podolski-Renić A, Lupšić E, Milosavljević S. Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:184-185.
https://hdl.handle.net/21.15107/rcub_cer_7270 .

Jadranin, Milka, Krstić, Gordana, Savić, Danica, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Pešić, Milica, Podolski-Renić, Ana, Lupšić, Ema, Milosavljević, Slobodan, "Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):184-185,
https://hdl.handle.net/21.15107/rcub_cer_7270 .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7268
AB  - The genus Euphorbia represents one of the largest and most diverse plant genera. Plant species of this genera are a rich source of biologically active compounds. Our previous investigation of E. palustris L. revealed an excellent anti-melanoma activity of 3β-benzoyloxy-13α-dodecanoyloxy-ingenol (1), and 3β,13α,17-tribenzoyloxy-ingenol (2), significantly better than ingenol-mebutate (Picato®), a commercial anti-melanoma agent approved by the U.S. Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the topical treatment of actinic keratosis [1]. Hence, in this research, using liquid chromatography–electrospray ionization mass spectrometry, chloroform-methanolic extracts of two samples of E. palustris collected at different localities in Serbia were analysed in order to confirm the presence of 1.Based on the tentative analysis of the LC-HRESI-MS and the previous examination of this plant species, ingenanes 1 and 2 were confirmed in both extracts examined. Additionally, both extracts contained several metabolites that structurally corresponds to ingenol, with a decanoyl group in their structure. Based on these preliminary results, our goal in further investigation is to isolate other ingenane molecules and test their anti-melanoma activity.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract
SP  - 43
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_cer_7268
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Savić, Danica and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "The genus Euphorbia represents one of the largest and most diverse plant genera. Plant species of this genera are a rich source of biologically active compounds. Our previous investigation of E. palustris L. revealed an excellent anti-melanoma activity of 3β-benzoyloxy-13α-dodecanoyloxy-ingenol (1), and 3β,13α,17-tribenzoyloxy-ingenol (2), significantly better than ingenol-mebutate (Picato®), a commercial anti-melanoma agent approved by the U.S. Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the topical treatment of actinic keratosis [1]. Hence, in this research, using liquid chromatography–electrospray ionization mass spectrometry, chloroform-methanolic extracts of two samples of E. palustris collected at different localities in Serbia were analysed in order to confirm the presence of 1.Based on the tentative analysis of the LC-HRESI-MS and the previous examination of this plant species, ingenanes 1 and 2 were confirmed in both extracts examined. Additionally, both extracts contained several metabolites that structurally corresponds to ingenol, with a decanoyl group in their structure. Based on these preliminary results, our goal in further investigation is to isolate other ingenane molecules and test their anti-melanoma activity.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract",
pages = "43-44",
url = "https://hdl.handle.net/21.15107/rcub_cer_7268"
}

Krstić, G., Jadranin, M., Savić, D., Novaković, M., Lekić, S., Tešević, V.,& Milosavljević, S.. (2023). LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 43-44.
https://hdl.handle.net/21.15107/rcub_cer_7268

Krstić G, Jadranin M, Savić D, Novaković M, Lekić S, Tešević V, Milosavljević S. LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:43-44.
https://hdl.handle.net/21.15107/rcub_cer_7268 .

Krstić, Gordana, Jadranin, Milka, Savić, Danica, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Milosavljević, Slobodan, "LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):43-44,
https://hdl.handle.net/21.15107/rcub_cer_7268 .

TY  - CONF
AU  - Novaković, Miroslav
AU  - Jadranin, Milka
AU  - Lekić, Stefan
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
AB  - Bisbibenzyls represent chemical class of compounds containing two bibenzyl units mutually connected by an oxygen atom and/or directly by the “C-C” bonds. They are chemical markers of liverworts, the ancestors of higher vascular plants. Marchantin A from Marchantia polymorpha was the first discovered bisbibenzyl. More than 120 chemically characterized bisbibenzyls 
were reported up to 2020 with numerous biological activities [1]. The first report on bisbibenzyls in higher (vascular) plants was published in 2007, when 
Kosenkova et al. (2007) isolated riccardin C in Primula veris subsp. macrocalyx from Mt. Altay in Russia as the dominant compound in the extract of the whole plant [2]. In the repeated study of the same species, Kosenkova et al. (2009) confirmed their findings discovering an additional bisbibenzyl constituent, perrottetin E [3].
In this investigation from the CH2Cl2/CH3OH (1:1) extract of the air-dried, powdered roots nine isbibenzyls, five new, have been isolated from P. veris subsp. columnae and P. acaulis using dry column flash chromatography followed by semipreparative HPLC chromatography and identified on the basis of 1D and 2D NMR, IR, UV and HRESIMS data. This investigation widens the knowledge about the presence of bisbibenzyls in vascular plants since only two bisbibenzyls have been previously found in vascular plants, in Primula veris subsp. macrocalyx.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Bisbibenzyls in Primula species
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_cer_7269
ER  - 

@conference{
author = "Novaković, Miroslav and Jadranin, Milka and Lekić, Stefan and Savić, Danica and Krstić, Gordana and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "Bisbibenzyls represent chemical class of compounds containing two bibenzyl units mutually connected by an oxygen atom and/or directly by the “C-C” bonds. They are chemical markers of liverworts, the ancestors of higher vascular plants. Marchantin A from Marchantia polymorpha was the first discovered bisbibenzyl. More than 120 chemically characterized bisbibenzyls 
were reported up to 2020 with numerous biological activities [1]. The first report on bisbibenzyls in higher (vascular) plants was published in 2007, when 
Kosenkova et al. (2007) isolated riccardin C in Primula veris subsp. macrocalyx from Mt. Altay in Russia as the dominant compound in the extract of the whole plant [2]. In the repeated study of the same species, Kosenkova et al. (2009) confirmed their findings discovering an additional bisbibenzyl constituent, perrottetin E [3].
In this investigation from the CH2Cl2/CH3OH (1:1) extract of the air-dried, powdered roots nine isbibenzyls, five new, have been isolated from P. veris subsp. columnae and P. acaulis using dry column flash chromatography followed by semipreparative HPLC chromatography and identified on the basis of 1D and 2D NMR, IR, UV and HRESIMS data. This investigation widens the knowledge about the presence of bisbibenzyls in vascular plants since only two bisbibenzyls have been previously found in vascular plants, in Primula veris subsp. macrocalyx.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Bisbibenzyls in Primula species",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_cer_7269"
}

Novaković, M., Jadranin, M., Lekić, S., Savić, D., Krstić, G., Tešević, V.,& Milosavljević, S.. (2023). Bisbibenzyls in Primula species. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 47-48.
https://hdl.handle.net/21.15107/rcub_cer_7269

Novaković M, Jadranin M, Lekić S, Savić D, Krstić G, Tešević V, Milosavljević S. Bisbibenzyls in Primula species. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:47-48.
https://hdl.handle.net/21.15107/rcub_cer_7269 .

Novaković, Miroslav, Jadranin, Milka, Lekić, Stefan, Savić, Danica, Krstić, Gordana, Tešević, Vele, Milosavljević, Slobodan, "Bisbibenzyls in Primula species" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):47-48,
https://hdl.handle.net/21.15107/rcub_cer_7269 .

TY  - CONF
AU  - Lekić, Stefan
AU  - Novaković, Miroslav
AU  - Savić, Danica
AU  - Тrendafilova, Antoaneta
AU  - Ivanova, Viktoria
AU  - Krstić, Gordana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7271
AB  - In this investigation nine known diarylheptanoids, namely platyphyllenone, hirsutenone, alnuside A and B, platyphylloside, oregonin, platyphyllonol-5-O-β-D-xylopyranoside, oregonoyl A and oregonoyl B have 
been isolated from the bark of A. incana using dry column flash chromatography followed by semipreparative HPLC chromatography. The structure elucidation has been performed using 1D and 2D NMR, as well as, IR and UV spectroscopy.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Diarylheptanoids from gray alder
SP  - 201
EP  - 200
UR  - https://hdl.handle.net/21.15107/rcub_cer_7271
ER  - 

@conference{
author = "Lekić, Stefan and Novaković, Miroslav and Savić, Danica and Тrendafilova, Antoaneta and Ivanova, Viktoria and Krstić, Gordana and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "In this investigation nine known diarylheptanoids, namely platyphyllenone, hirsutenone, alnuside A and B, platyphylloside, oregonin, platyphyllonol-5-O-β-D-xylopyranoside, oregonoyl A and oregonoyl B have 
been isolated from the bark of A. incana using dry column flash chromatography followed by semipreparative HPLC chromatography. The structure elucidation has been performed using 1D and 2D NMR, as well as, IR and UV spectroscopy.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Diarylheptanoids from gray alder",
pages = "201-200",
url = "https://hdl.handle.net/21.15107/rcub_cer_7271"
}

Lekić, S., Novaković, M., Savić, D., Тrendafilova, A., Ivanova, V., Krstić, G., Tešević, V.,& Milosavljević, S.. (2023). Diarylheptanoids from gray alder. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 201-200.
https://hdl.handle.net/21.15107/rcub_cer_7271

Lekić S, Novaković M, Savić D, Тrendafilova A, Ivanova V, Krstić G, Tešević V, Milosavljević S. Diarylheptanoids from gray alder. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:201-200.
https://hdl.handle.net/21.15107/rcub_cer_7271 .

Lekić, Stefan, Novaković, Miroslav, Savić, Danica, Тrendafilova, Antoaneta, Ivanova, Viktoria, Krstić, Gordana, Tešević, Vele, Milosavljević, Slobodan, "Diarylheptanoids from gray alder" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):201-200,
https://hdl.handle.net/21.15107/rcub_cer_7271 .

TY  - CONF
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7272
AB  - The diversity of plant species from the genus Euphorbia and wide variety of secondary metabolites they produce contributed to their successful use in the traditional medicine all over the world. Therapeutic applications have been attributed to different compounds such as diterpenes and triterpenes. Previous investigations revealed that Euphorbia diterpenes possess a variety of biological activities such as anti-inflammatory, antitumor and antiviral. Some of them showed that tigliane diterpenes, beside other potential pharmaceutical activities, inhibited wild-type HIV-1 and HIV-2 strains as well as drug resistant strains of HIV 1 and that they exhibited low cytotoxicity. Two tigliane diterpenes were 
isolated from the latex of E. lucida, using classical and instrumental chromatographic techniques. ESI-HRMS spectra showed that both isolated compounds possess the same molecular formula (C31H38O6). 1D NMR spectra revealed benzoate and isobutanoate ester groups in both molecules, Using HMBC spectra their position was determined: C-13 for isobutanoate and C-20 for benzoate. Exact configurations were discovered by NOE correlations. It was concluded that these two molecules differ in the way of the binding the five-membered and seven-membered rings, i.e., that these two molecules are C-4-epimers. Compound 1 (20-benzoyloxy-13α-isobutanoyloxy-4,12-dideoxyphorbol) was already described in the literature as a metabolite of E. pannonica [1], while compound 2 (20-benzoyloxy-13α-isobutanoyloxy-4-epi-4,12-dideoxyphorbol) represents a new tigliane derivative.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Tigliane diterpenes isolated from the latex of Euphorbia lucida
SP  - 239
EP  - 239
UR  - https://hdl.handle.net/21.15107/rcub_cer_7272
ER  - 

@conference{
author = "Savić, Danica and Krstić, Gordana and Jadranin, Milka and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "The diversity of plant species from the genus Euphorbia and wide variety of secondary metabolites they produce contributed to their successful use in the traditional medicine all over the world. Therapeutic applications have been attributed to different compounds such as diterpenes and triterpenes. Previous investigations revealed that Euphorbia diterpenes possess a variety of biological activities such as anti-inflammatory, antitumor and antiviral. Some of them showed that tigliane diterpenes, beside other potential pharmaceutical activities, inhibited wild-type HIV-1 and HIV-2 strains as well as drug resistant strains of HIV 1 and that they exhibited low cytotoxicity. Two tigliane diterpenes were 
isolated from the latex of E. lucida, using classical and instrumental chromatographic techniques. ESI-HRMS spectra showed that both isolated compounds possess the same molecular formula (C31H38O6). 1D NMR spectra revealed benzoate and isobutanoate ester groups in both molecules, Using HMBC spectra their position was determined: C-13 for isobutanoate and C-20 for benzoate. Exact configurations were discovered by NOE correlations. It was concluded that these two molecules differ in the way of the binding the five-membered and seven-membered rings, i.e., that these two molecules are C-4-epimers. Compound 1 (20-benzoyloxy-13α-isobutanoyloxy-4,12-dideoxyphorbol) was already described in the literature as a metabolite of E. pannonica [1], while compound 2 (20-benzoyloxy-13α-isobutanoyloxy-4-epi-4,12-dideoxyphorbol) represents a new tigliane derivative.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Tigliane diterpenes isolated from the latex of Euphorbia lucida",
pages = "239-239",
url = "https://hdl.handle.net/21.15107/rcub_cer_7272"
}

Savić, D., Krstić, G., Jadranin, M., Novaković, M., Lekić, S., Tešević, V.,& Milosavljević, S.. (2023). Tigliane diterpenes isolated from the latex of Euphorbia lucida. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 239-239.
https://hdl.handle.net/21.15107/rcub_cer_7272

Savić D, Krstić G, Jadranin M, Novaković M, Lekić S, Tešević V, Milosavljević S. Tigliane diterpenes isolated from the latex of Euphorbia lucida. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:239-239.
https://hdl.handle.net/21.15107/rcub_cer_7272 .

Savić, Danica, Krstić, Gordana, Jadranin, Milka, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Milosavljević, Slobodan, "Tigliane diterpenes isolated from the latex of Euphorbia lucida" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):239-239,
https://hdl.handle.net/21.15107/rcub_cer_7272 .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Ilić-Tomić, Tatjana
AU  - Đorđević, Iris
AU  - Anđelković, Boban D.
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Asakawa, Yoshinori
PY  - 2023
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7188
AB  - Bisbibenzyls are specialized metabolites found exclusively in liverworts, until recently; they represent chemical markers of liverworts. Their occurrence in vascular plants was noticed in 2007, when they were found in Primula veris subsp. macrocalyx from Russia. This report prompted us to chemically analyze the two most common Serbian Primula species, P. veris subsp. columnae and P. acaulis, in order to determine the presence of bisbibenzyls in them. Our study revealed nine structurally distinct bisbibenzyls (1–9), identified based on 1D and 2D NMR, IR, UV and HRESIMS data. Among them were five previously undescribed compounds (2–6). The remaining com­ pounds found and previously described in the literature were: the bisbibenzyls riccardin C (1), isoperrottetin A (7), isoplagiochin E (8) and 11-O-demethylmarchantin I (9), as well as 4-hydroxyphenylmethylketone (10) and 4-hydroxy-3-methoxyphenylmethylketone (11). Riccardin C was the most dominant bisbibenzyl in both species studied. Previously, it was the first bisbibenzyl found in vascular plants (P. veris subsp. macrocalyx). An assessment of the cytotoxic activity of the isolated compounds against A549 lung cancer and healthy MRC5 cell lines was also the subject of our study. Compounds 6 and 9 exhibited significant cytotoxic activity expressed by IC50 values of 12 μM, but the selectivity was not satisfactory.
PB  - Elsevier
T2  - Phytochemistry
T1  - Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L
VL  - 212
SP  - 113719
DO  - 10.1016/j.phytochem.2023.113719
ER  - 

@article{
author = "Novaković, Miroslav and Ilić-Tomić, Tatjana and Đorđević, Iris and Anđelković, Boban D. and Tešević, Vele and Milosavljević, Slobodan and Asakawa, Yoshinori",
year = "2023, 2023",
abstract = "Bisbibenzyls are specialized metabolites found exclusively in liverworts, until recently; they represent chemical markers of liverworts. Their occurrence in vascular plants was noticed in 2007, when they were found in Primula veris subsp. macrocalyx from Russia. This report prompted us to chemically analyze the two most common Serbian Primula species, P. veris subsp. columnae and P. acaulis, in order to determine the presence of bisbibenzyls in them. Our study revealed nine structurally distinct bisbibenzyls (1–9), identified based on 1D and 2D NMR, IR, UV and HRESIMS data. Among them were five previously undescribed compounds (2–6). The remaining com­ pounds found and previously described in the literature were: the bisbibenzyls riccardin C (1), isoperrottetin A (7), isoplagiochin E (8) and 11-O-demethylmarchantin I (9), as well as 4-hydroxyphenylmethylketone (10) and 4-hydroxy-3-methoxyphenylmethylketone (11). Riccardin C was the most dominant bisbibenzyl in both species studied. Previously, it was the first bisbibenzyl found in vascular plants (P. veris subsp. macrocalyx). An assessment of the cytotoxic activity of the isolated compounds against A549 lung cancer and healthy MRC5 cell lines was also the subject of our study. Compounds 6 and 9 exhibited significant cytotoxic activity expressed by IC50 values of 12 μM, but the selectivity was not satisfactory.",
publisher = "Elsevier",
journal = "Phytochemistry",
title = "Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L",
volume = "212",
pages = "113719",
doi = "10.1016/j.phytochem.2023.113719"
}

Novaković, M., Ilić-Tomić, T., Đorđević, I., Anđelković, B. D., Tešević, V., Milosavljević, S.,& Asakawa, Y.. (2023). Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L. in Phytochemistry
Elsevier., 212, 113719.
https://doi.org/10.1016/j.phytochem.2023.113719

Novaković M, Ilić-Tomić T, Đorđević I, Anđelković BD, Tešević V, Milosavljević S, Asakawa Y. Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L. in Phytochemistry. 2023;212:113719.
doi:10.1016/j.phytochem.2023.113719 .

Novaković, Miroslav, Ilić-Tomić, Tatjana, Đorđević, Iris, Anđelković, Boban D., Tešević, Vele, Milosavljević, Slobodan, Asakawa, Yoshinori, "Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L" in Phytochemistry, 212 (2023):113719,
https://doi.org/10.1016/j.phytochem.2023.113719 . .

TY  - JOUR
AU  - Gospodinova, Z.
AU  - Antov, G.
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Krasteva, N.
AU  - Pavlov, D.
AU  - Valcheva-Kuzmanova, S.
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7225
AB  - Background and objective: Cotinus coggygria Scop. is a valuable medicinal  plant species with pronounced pharmacological potential due to its numerous biological  activities. The herb is characterized by a high content of polyphenols among which is fustin.  The anticancer activities of fustin, however, are extremely weakly studied. The aim of the  present study was to investigate the in vitro antiproliferative potential of fustin isolated from  the heartwood of C. coggygria against cell lines originating from two of the most common  cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials  and methods: Cell growth inhibitory properties of fustin were examined by MTT assay.  Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin  cell line. Results: The obtained results showed that fustin reduced the proliferation of  all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer  MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was  least aff ected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer  MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological  alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic  ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated  from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative  properties against breast cancer cell line MDA-MB-231 which will be a subject of our more  detailed future investigations.
PB  - Walter de Gruyter GmbH
T2  - Acta Medica Bulgarica
T1  - Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines
VL  - 50
IS  - 1
SP  - 27
EP  - 33
DO  - 10.2478/amb-2023-0004
ER  - 

@article{
author = "Gospodinova, Z. and Antov, G. and Novaković, Miroslav and Tešević, Vele and Krasteva, N. and Pavlov, D. and Valcheva-Kuzmanova, S.",
year = "2023",
abstract = "Background and objective: Cotinus coggygria Scop. is a valuable medicinal  plant species with pronounced pharmacological potential due to its numerous biological  activities. The herb is characterized by a high content of polyphenols among which is fustin.  The anticancer activities of fustin, however, are extremely weakly studied. The aim of the  present study was to investigate the in vitro antiproliferative potential of fustin isolated from  the heartwood of C. coggygria against cell lines originating from two of the most common  cancer types – breast (MDA-MB-231 and MCF7), and colon cancer (Colon 26). Materials  and methods: Cell growth inhibitory properties of fustin were examined by MTT assay.  Subsequently, phase-contrast and fl uorescence microscopy analysis as well as colonyforming assay were carried out on the most sensitive to the cytostatic action of the fustin  cell line. Results: The obtained results showed that fustin reduced the proliferation of  all studied cell lines. The highest cytostatic eff ect was registered towards breast cancer  MDA-MB-231 cells with a half maximal inhibitory concentration (IC50) value of 56.02 μg/ ml followed by colon cancer cells with an IC50 of 78.07 μg/ml. MCF7 cell proliferation was  least aff ected with a calculated IC50 of 187.8 μg/ml. Further investigations on breast cancer  MDA-MB-231 cells indicated decreased density of cell monolayer and some morphological  alterations, signifi cant attenuation in the number of viable cells, and diminished clonogenic  ability of cells after fustin exposure. Conclusion: It could be concluded that fustin isolated  from the heartwood of medicinal plant C. coggygria possesses marked antiproliferative  properties against breast cancer cell line MDA-MB-231 which will be a subject of our more  detailed future investigations.",
publisher = "Walter de Gruyter GmbH",
journal = "Acta Medica Bulgarica",
title = "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines",
volume = "50",
number = "1",
pages = "27-33",
doi = "10.2478/amb-2023-0004"
}

Gospodinova, Z., Antov, G., Novaković, M., Tešević, V., Krasteva, N., Pavlov, D.,& Valcheva-Kuzmanova, S.. (2023). Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica
Walter de Gruyter GmbH., 50(1), 27-33.
https://doi.org/10.2478/amb-2023-0004

Gospodinova Z, Antov G, Novaković M, Tešević V, Krasteva N, Pavlov D, Valcheva-Kuzmanova S. Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines. in Acta Medica Bulgarica. 2023;50(1):27-33.
doi:10.2478/amb-2023-0004 .

Gospodinova, Z., Antov, G., Novaković, Miroslav, Tešević, Vele, Krasteva, N., Pavlov, D., Valcheva-Kuzmanova, S., "Antiproliferative Activity of Natural Flavonoid Fustin Isolated from the Heartwood of                    Cotinus Coggygria                    Scop. Against Breast and Colon Cancer Cell Lines" in Acta Medica Bulgarica, 50, no. 1 (2023):27-33,
https://doi.org/10.2478/amb-2023-0004 . .

TY  - JOUR
AU  - Simić, Katarina
AU  - Todorović, Nina
AU  - Trifunović, Snežana
AU  - Miladinović, Zoran P.
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Avramović, Nataša
AU  - Gođevac, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Tasić, Ljubica
AU  - Mandić, Boris
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5199
AB  - Schizophrenia is a widespread mental disorder that leads to significant functional impairments and premature death. The state of the art indicates gaps in the understanding and diagnosis of this disease, but also the need for personalized and precise approaches to patients through customized medical treatment and reliable monitoring of treatment response. In order to fulfill existing gaps, the establishment of a universal set of disorder biomarkers is a necessary step. Metabolomic investigations of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39), based on NMR analyses associated with chemometrics, led to the identification of 26 metabolites/biomarkers for this disorder. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models with prediction accuracies of 0.9718 and higher were accomplished during chemometric analysis. The established biomarker set includes aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols, which are identified for the first time in schizophrenia serum samples by NMR experiments. The other 22 identified metabolites in the Serbian samples are in accordance with the previously established NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. Thirteen metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) that are common for three different ethnic and geographic origins (Serbia, Brazil and China) could be a good start point for the setup of a universal NMR serum biomarker set for schizophrenia.
PB  - MDPI
T2  - Metabolites
T1  - NMR Metabolomics in Serum Fingerprinting of Schizophrenia Patients in a Serbian Cohort
VL  - 12
IS  - 8
SP  - 707
DO  - 10.3390/metabo12080707
ER  - 

@article{
author = "Simić, Katarina and Todorović, Nina and Trifunović, Snežana and Miladinović, Zoran P. and Gavrilović, Aleksandra and Jovanović, Silvana and Avramović, Nataša and Gođevac, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Tasić, Ljubica and Mandić, Boris",
year = "2022",
abstract = "Schizophrenia is a widespread mental disorder that leads to significant functional impairments and premature death. The state of the art indicates gaps in the understanding and diagnosis of this disease, but also the need for personalized and precise approaches to patients through customized medical treatment and reliable monitoring of treatment response. In order to fulfill existing gaps, the establishment of a universal set of disorder biomarkers is a necessary step. Metabolomic investigations of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39), based on NMR analyses associated with chemometrics, led to the identification of 26 metabolites/biomarkers for this disorder. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models with prediction accuracies of 0.9718 and higher were accomplished during chemometric analysis. The established biomarker set includes aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols, which are identified for the first time in schizophrenia serum samples by NMR experiments. The other 22 identified metabolites in the Serbian samples are in accordance with the previously established NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. Thirteen metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) that are common for three different ethnic and geographic origins (Serbia, Brazil and China) could be a good start point for the setup of a universal NMR serum biomarker set for schizophrenia.",
publisher = "MDPI",
journal = "Metabolites",
title = "NMR Metabolomics in Serum Fingerprinting of Schizophrenia Patients in a Serbian Cohort",
volume = "12",
number = "8",
pages = "707",
doi = "10.3390/metabo12080707"
}

Simić, K., Todorović, N., Trifunović, S., Miladinović, Z. P., Gavrilović, A., Jovanović, S., Avramović, N., Gođevac, D., Vujisić, L., Tešević, V., Tasić, L.,& Mandić, B.. (2022). NMR Metabolomics in Serum Fingerprinting of Schizophrenia Patients in a Serbian Cohort. in Metabolites
MDPI., 12(8), 707.
https://doi.org/10.3390/metabo12080707

Simić K, Todorović N, Trifunović S, Miladinović ZP, Gavrilović A, Jovanović S, Avramović N, Gođevac D, Vujisić L, Tešević V, Tasić L, Mandić B. NMR Metabolomics in Serum Fingerprinting of Schizophrenia Patients in a Serbian Cohort. in Metabolites. 2022;12(8):707.
doi:10.3390/metabo12080707 .

Simić, Katarina, Todorović, Nina, Trifunović, Snežana, Miladinović, Zoran P., Gavrilović, Aleksandra, Jovanović, Silvana, Avramović, Nataša, Gođevac, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Tasić, Ljubica, Mandić, Boris, "NMR Metabolomics in Serum Fingerprinting of Schizophrenia Patients in a Serbian Cohort" in Metabolites, 12, no. 8 (2022):707,
https://doi.org/10.3390/metabo12080707 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Lončar, Biljana
AU  - Stanković Jeremić, Jovana
AU  - Cvetković, Mirjana
AU  - Pezo, Lato
AU  - Pezo, Milada
AU  - Todosijević, Marina
AU  - Tešević, Vele
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5409
AB  - Lavandula sp. essential oil and hydrolate are commercially valuable in various industry
branches with the potential for wide-ranging applications. This study aimed to evaluate the quality
of these products obtained from L. x intermedia cv. ‘Budrovka’ for the first time cultivated on Fruška
Gora Mt. (Serbia) during three successive seasons (2019, 2020, and 2021). Essential oil extraction
was obtained by steam distillation, and the composition and influence of weather conditions were
also assessed, using flowering tops. The obtained essential oils and hydrolates were analysed by
gas chromatography with a flame ionization detector (GC-FID) and gas chromatography coupled to
mass spectrometry (GC-MS). A linear regression model was developed to predict L. x intermedia cv.
‘Budrovka’ essential oil volatile compound content and hydrolate composition during three years,
according to temperature and precipitation data, and the appropriate regression coefficients were
calculated, while the correlation analysis was employed to analyse the correlations in hydrolate
and essential oil compounds. To completely describe the structure of the research data that would
present a better insight into the similarities and differences among the diverse L. x intermedia cv.
‘Budrovka’ samples, the PCA was used. The most dominant in L. intermedia cv. ‘Budrovka’ essential
oil and hydrolate were oxygenated monoterpenes: linalool, 1,8-cineole, borneol, linalyl acetate, and
terpinene-4-ol. It is established that the temperature was positively correlated with all essential oil
and hydrolate compounds. The precipitations were positively correlated with the main compounds
(linalool, 1,8-cineole, and borneol), while the other compounds’ content negatively correlated to
precipitation. The results indicated that Fruška Gora Mt. has suitable agro-ecological requirements
for cultivating Lavandula sp. and providing satisfactory essential oil and hydrolate.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Horticulturae
T1  - Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality
VL  - 8
SP  - 281
DO  - 10.3390/horticulturae8040281
ER  - 

@article{
author = "Aćimović, Milica and Lončar, Biljana and Stanković Jeremić, Jovana and Cvetković, Mirjana and Pezo, Lato and Pezo, Milada and Todosijević, Marina and Tešević, Vele",
year = "2022",
abstract = "Lavandula sp. essential oil and hydrolate are commercially valuable in various industry
branches with the potential for wide-ranging applications. This study aimed to evaluate the quality
of these products obtained from L. x intermedia cv. ‘Budrovka’ for the first time cultivated on Fruška
Gora Mt. (Serbia) during three successive seasons (2019, 2020, and 2021). Essential oil extraction
was obtained by steam distillation, and the composition and influence of weather conditions were
also assessed, using flowering tops. The obtained essential oils and hydrolates were analysed by
gas chromatography with a flame ionization detector (GC-FID) and gas chromatography coupled to
mass spectrometry (GC-MS). A linear regression model was developed to predict L. x intermedia cv.
‘Budrovka’ essential oil volatile compound content and hydrolate composition during three years,
according to temperature and precipitation data, and the appropriate regression coefficients were
calculated, while the correlation analysis was employed to analyse the correlations in hydrolate
and essential oil compounds. To completely describe the structure of the research data that would
present a better insight into the similarities and differences among the diverse L. x intermedia cv.
‘Budrovka’ samples, the PCA was used. The most dominant in L. intermedia cv. ‘Budrovka’ essential
oil and hydrolate were oxygenated monoterpenes: linalool, 1,8-cineole, borneol, linalyl acetate, and
terpinene-4-ol. It is established that the temperature was positively correlated with all essential oil
and hydrolate compounds. The precipitations were positively correlated with the main compounds
(linalool, 1,8-cineole, and borneol), while the other compounds’ content negatively correlated to
precipitation. The results indicated that Fruška Gora Mt. has suitable agro-ecological requirements
for cultivating Lavandula sp. and providing satisfactory essential oil and hydrolate.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Horticulturae",
title = "Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality",
volume = "8",
pages = "281",
doi = "10.3390/horticulturae8040281"
}

Aćimović, M., Lončar, B., Stanković Jeremić, J., Cvetković, M., Pezo, L., Pezo, M., Todosijević, M.,& Tešević, V.. (2022). Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality. in Horticulturae
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 8, 281.
https://doi.org/10.3390/horticulturae8040281

Aćimović M, Lončar B, Stanković Jeremić J, Cvetković M, Pezo L, Pezo M, Todosijević M, Tešević V. Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality. in Horticulturae. 2022;8:281.
doi:10.3390/horticulturae8040281 .

Aćimović, Milica, Lončar, Biljana, Stanković Jeremić, Jovana, Cvetković, Mirjana, Pezo, Lato, Pezo, Milada, Todosijević, Marina, Tešević, Vele, "Weather Conditions Influence on Lavandin Essential Oil and Hydrolate Quality" in Horticulturae, 8 (2022):281,
https://doi.org/10.3390/horticulturae8040281 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Pezo, Lato
AU  - Čabarkapa, Ivana
AU  - Trudić, Anika
AU  - Stanković Jeremić, Jovana
AU  - Varga, Ana
AU  - Lončar, Biljana
AU  - Šovljanski, Olja
AU  - Tešević, Vele
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5587
AB  - This study aimed to investigate the chemical composition of steam distillate essential oil and corresponding hydrolate obtained from S. officinalis grown in Serbia, as well as the influence of weather conditions (temperature and precipitations) on their chemical profiles. Furthermore, their antimicrobial activity was investigated in vitro. The main compounds in essential oil were cis-thujone, followed by camphor, trans-thujone, and 1,8-cineole, while hydrolate was slightly different from the essential oil, with camphor, cis-thujone, and 1,8-cineole as the main compounds. Among the eight respiratory-associated microorganisms, Klebsiella oxytoca was the most sensitive to the tested EOs (minimum inhibitory concentration (MIC)/minimal bactericidal/fungicidal concentration (MBC/MFC) were 14.20 and 28.4 μL mL−1, respectively). MIC and MBC values of other tested bacteria ranged between 28.40 and 227.25 μL mL−1 while for Candida albicans MIC/MFC ranged from 28.40/56.81 to 56.81–113.63 μL mL−1. Antibiotic susceptibility patterns for the analyzed eight respiratory-associated microorganisms showed an intermediate level of resistance to commonly used antibiotics such as ampicillin, levofloxacin, and ciprofloxacin. As a preliminary approach to the antimicrobial profiling of the tested EO, the obtained results revealed that the tested samples possess remarkable antibacterial activities and could be used to develop pharmaceutical formulations as an alternative to conventional antibiotic therapy.
PB  - MDPI AG
T2  - Processes
T1  - Variation of Salvia officinalis L. Essential Oil and Hydrolate Composition and Their Antimicrobial Activity
VL  - 10
IS  - 8
SP  - 1608
DO  - 10.3390/pr10081608
ER  - 

@article{
author = "Aćimović, Milica and Pezo, Lato and Čabarkapa, Ivana and Trudić, Anika and Stanković Jeremić, Jovana and Varga, Ana and Lončar, Biljana and Šovljanski, Olja and Tešević, Vele",
year = "2022",
abstract = "This study aimed to investigate the chemical composition of steam distillate essential oil and corresponding hydrolate obtained from S. officinalis grown in Serbia, as well as the influence of weather conditions (temperature and precipitations) on their chemical profiles. Furthermore, their antimicrobial activity was investigated in vitro. The main compounds in essential oil were cis-thujone, followed by camphor, trans-thujone, and 1,8-cineole, while hydrolate was slightly different from the essential oil, with camphor, cis-thujone, and 1,8-cineole as the main compounds. Among the eight respiratory-associated microorganisms, Klebsiella oxytoca was the most sensitive to the tested EOs (minimum inhibitory concentration (MIC)/minimal bactericidal/fungicidal concentration (MBC/MFC) were 14.20 and 28.4 μL mL−1, respectively). MIC and MBC values of other tested bacteria ranged between 28.40 and 227.25 μL mL−1 while for Candida albicans MIC/MFC ranged from 28.40/56.81 to 56.81–113.63 μL mL−1. Antibiotic susceptibility patterns for the analyzed eight respiratory-associated microorganisms showed an intermediate level of resistance to commonly used antibiotics such as ampicillin, levofloxacin, and ciprofloxacin. As a preliminary approach to the antimicrobial profiling of the tested EO, the obtained results revealed that the tested samples possess remarkable antibacterial activities and could be used to develop pharmaceutical formulations as an alternative to conventional antibiotic therapy.",
publisher = "MDPI AG",
journal = "Processes",
title = "Variation of Salvia officinalis L. Essential Oil and Hydrolate Composition and Their Antimicrobial Activity",
volume = "10",
number = "8",
pages = "1608",
doi = "10.3390/pr10081608"
}

Aćimović, M., Pezo, L., Čabarkapa, I., Trudić, A., Stanković Jeremić, J., Varga, A., Lončar, B., Šovljanski, O.,& Tešević, V.. (2022). Variation of Salvia officinalis L. Essential Oil and Hydrolate Composition and Their Antimicrobial Activity. in Processes
MDPI AG., 10(8), 1608.
https://doi.org/10.3390/pr10081608

Aćimović M, Pezo L, Čabarkapa I, Trudić A, Stanković Jeremić J, Varga A, Lončar B, Šovljanski O, Tešević V. Variation of Salvia officinalis L. Essential Oil and Hydrolate Composition and Their Antimicrobial Activity. in Processes. 2022;10(8):1608.
doi:10.3390/pr10081608 .

Aćimović, Milica, Pezo, Lato, Čabarkapa, Ivana, Trudić, Anika, Stanković Jeremić, Jovana, Varga, Ana, Lončar, Biljana, Šovljanski, Olja, Tešević, Vele, "Variation of Salvia officinalis L. Essential Oil and Hydrolate Composition and Their Antimicrobial Activity" in Processes, 10, no. 8 (2022):1608,
https://doi.org/10.3390/pr10081608 . .