TY  - JOUR
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Špirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Lazić, Vesna
AU  - Žižak, Željko
AU  - Vunduk, Jovana
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7297
AB  - Growing mushrooms means meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Customized strains adapted to local conditions are urgently needed. Before introducing new species to the strain development pipeline, the chemical characterization and biological activity of wild ones need to be assessed. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated including: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and anti-angiotensin converting enzyme (ACE). The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to normal cells. The evaluated species provided a solid foundation for commercial development while keeping local ecology in mind.
PB  - MDPI
T2  - Molecules
T1  - Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans
VL  - 29
IS  - 2
SP  - 314
DO  - 10.3390/molecules29020314
ER  - 

@article{
author = "Kozarski, Maja and Klaus, Anita and Špirović Trifunović, Bojana and Miletić, Srđan and Lazić, Vesna and Žižak, Željko and Vunduk, Jovana",
year = "2024",
abstract = "Growing mushrooms means meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Customized strains adapted to local conditions are urgently needed. Before introducing new species to the strain development pipeline, the chemical characterization and biological activity of wild ones need to be assessed. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated including: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and anti-angiotensin converting enzyme (ACE). The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to normal cells. The evaluated species provided a solid foundation for commercial development while keeping local ecology in mind.",
publisher = "MDPI",
journal = "Molecules",
title = "Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans",
volume = "29",
number = "2",
pages = "314",
doi = "10.3390/molecules29020314"
}

Kozarski, M., Klaus, A., Špirović Trifunović, B., Miletić, S., Lazić, V., Žižak, Ž.,& Vunduk, J.. (2024). Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans. in Molecules
MDPI., 29(2), 314.
https://doi.org/10.3390/molecules29020314

Kozarski M, Klaus A, Špirović Trifunović B, Miletić S, Lazić V, Žižak Ž, Vunduk J. Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans. in Molecules. 2024;29(2):314.
doi:10.3390/molecules29020314 .

Kozarski, Maja, Klaus, Anita, Špirović Trifunović, Bojana, Miletić, Srđan, Lazić, Vesna, Žižak, Željko, Vunduk, Jovana, "Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans" in Molecules, 29, no. 2 (2024):314,
https://doi.org/10.3390/molecules29020314 . .

TY  - GEN
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Špirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Lazić, Vesna
AU  - Žižak, Željko
AU  - Vunduk, Jovana
PY  - 2023
UR  - https://www.preprints.org/manuscript/202311.1765/v1
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7057
AB  - Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.
T2  - Preprints
T1  - Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation
SP  - 2023111765
DO  - 10.20944/preprints202311.1765.v1
ER  - 

@misc{
author = "Kozarski, Maja and Klaus, Anita and Špirović Trifunović, Bojana and Miletić, Srđan and Lazić, Vesna and Žižak, Željko and Vunduk, Jovana",
year = "2023",
abstract = "Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.",
journal = "Preprints",
title = "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation",
pages = "2023111765",
doi = "10.20944/preprints202311.1765.v1"
}

Kozarski, M., Klaus, A., Špirović Trifunović, B., Miletić, S., Lazić, V., Žižak, Ž.,& Vunduk, J.. (2023). Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation. in Preprints, 2023111765.
https://doi.org/10.20944/preprints202311.1765.v1

Kozarski M, Klaus A, Špirović Trifunović B, Miletić S, Lazić V, Žižak Ž, Vunduk J. Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation. in Preprints. 2023;:2023111765.
doi:10.20944/preprints202311.1765.v1 .

Kozarski, Maja, Klaus, Anita, Špirović Trifunović, Bojana, Miletić, Srđan, Lazić, Vesna, Žižak, Željko, Vunduk, Jovana, "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation" in Preprints (2023):2023111765,
https://doi.org/10.20944/preprints202311.1765.v1 . .

TY  - DATA
AU  - Kozarski, Maja
AU  - Klaus, Anita
AU  - Špirović Trifunović, Bojana
AU  - Miletić, Srđan
AU  - Lazić, Vesna
AU  - Žižak, Željko
AU  - Vunduk, Jovana
PY  - 2023
UR  - https://www.preprints.org/manuscript/202311.1765/v1
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7057
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7058
AB  - Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.
T2  - Preprints
T1  - Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation"
DO  - 10.20944/preprints202311.1765.v1
ER  - 

@misc{
author = "Kozarski, Maja and Klaus, Anita and Špirović Trifunović, Bojana and Miletić, Srđan and Lazić, Vesna and Žižak, Željko and Vunduk, Jovana",
year = "2023",
abstract = "Growing mushrooms is meeting challenges while aiming for sustainability and circularity. Wherever the producer is located, commercial strains are the same originating from several producers. Following the harvest, enormous quantities of spent mushroom substrate containing spores are disposed presenting a type of foreign material pressure on the ecosystem in the form of the loss of genetic diversity in wild mushroom populations. This challenge can be mitigated by bioprospecting local strains and using them to generate commercial inoculum. Accordingly, the mycoceutical potential of five polypore mushroom species from Serbia was evaluated: secondary metabolite composition, oxidative damage prevention, anti-tyrosinase, and antiangiotensin converting enzyme (ACE) properties. The phenolic pattern was comparable in all samples, but the amounts of specific chemicals varied. Hydroxybenzoic acids were the primary components. All samples had varying quantities of ascorbic acid, carotene, and lycopene, and showed a pronounced inhibition of lipid peroxidation (LPx) and ability to scavenge HO•. Extracts were more potent tyrosinase inhibitors but unsuccessful when faced with ACE. Fomitopsis pinicola had the strongest anti-tumor efficacy while Ganoderma lucidum demonstrated strong selectivity in anti-tumor effect in comparison to healthy cells. The evaluated species provided a solid foundation for commercial development while keeping the local ecology in mind.",
journal = "Preprints",
title = "Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation"",
doi = "10.20944/preprints202311.1765.v1"
}

Kozarski, M., Klaus, A., Špirović Trifunović, B., Miletić, S., Lazić, V., Žižak, Ž.,& Vunduk, J.. (2023). Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation". in Preprints.
https://doi.org/10.20944/preprints202311.1765.v1

Kozarski M, Klaus A, Špirović Trifunović B, Miletić S, Lazić V, Žižak Ž, Vunduk J. Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation". in Preprints. 2023;.
doi:10.20944/preprints202311.1765.v1 .

Kozarski, Maja, Klaus, Anita, Špirović Trifunović, Bojana, Miletić, Srđan, Lazić, Vesna, Žižak, Željko, Vunduk, Jovana, "Supplementary information for: "Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation"" in Preprints (2023),
https://doi.org/10.20944/preprints202311.1765.v1 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Dojčinović, Biljana
AU  - Špirović Trifunović, Bojana
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5617
AB  - The aim of the current research was to determine the content of (potentially) toxic elements and insecticide residues in monofloral sunflower bee-collected pollen. For micro- and trace elements determination Inductively Coupled Plasma Optical Emission (ICP-OES) analytical method was used while insecticide residue content was monitored by applying Liquid Chromatography-Mass Spectrometry (LC-MS/MS) technique. In total, seventeen micro/trace elements were quantified. None of the twenty four examined insecticides were detected above the limit of detection (LOD) which makes studied sunflower bee-collected pollen eco-friendly both to bees and humans. Based on presence of several toxic as well as potentially toxic elements calculations for estimated weekly intakes (EWI), and oral intakes (OI) were made and used for health risk assessment based on the computation of two different health risk quotients (HQ)- acute (HQA) and long-term (HQL). The obtained results proved that all HQ values for adults were negligible or low except in case of HQL value for arsenic (0.32) which can be characterized as medium. However, in case of children much more precaution is needed due to significant HQL risk for arsenic (1.511). The attained data can help to make additional linkage between bee-collected pollen as food ingredients and potential benefits/risks for human health.
PB  - Informa UK Limited
T2  - Journal of Environmental Science and Health, Part B
T1  - Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment
VL  - 57
IS  - 7
SP  - 568
EP  - 575
DO  - 10.1080/03601234.2022.2079348
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Dojčinović, Biljana and Špirović Trifunović, Bojana and Milinčić, Danijel D. and Nedić, Nebojša and Stanojević, Sladjana and Pešić, Mirjana",
year = "2022",
abstract = "The aim of the current research was to determine the content of (potentially) toxic elements and insecticide residues in monofloral sunflower bee-collected pollen. For micro- and trace elements determination Inductively Coupled Plasma Optical Emission (ICP-OES) analytical method was used while insecticide residue content was monitored by applying Liquid Chromatography-Mass Spectrometry (LC-MS/MS) technique. In total, seventeen micro/trace elements were quantified. None of the twenty four examined insecticides were detected above the limit of detection (LOD) which makes studied sunflower bee-collected pollen eco-friendly both to bees and humans. Based on presence of several toxic as well as potentially toxic elements calculations for estimated weekly intakes (EWI), and oral intakes (OI) were made and used for health risk assessment based on the computation of two different health risk quotients (HQ)- acute (HQA) and long-term (HQL). The obtained results proved that all HQ values for adults were negligible or low except in case of HQL value for arsenic (0.32) which can be characterized as medium. However, in case of children much more precaution is needed due to significant HQL risk for arsenic (1.511). The attained data can help to make additional linkage between bee-collected pollen as food ingredients and potential benefits/risks for human health.",
publisher = "Informa UK Limited",
journal = "Journal of Environmental Science and Health, Part B",
title = "Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment",
volume = "57",
number = "7",
pages = "568-575",
doi = "10.1080/03601234.2022.2079348"
}

Kostić, A. Ž., Dojčinović, B., Špirović Trifunović, B., Milinčić, D. D., Nedić, N., Stanojević, S.,& Pešić, M.. (2022). Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment. in Journal of Environmental Science and Health, Part B
Informa UK Limited., 57(7), 568-575.
https://doi.org/10.1080/03601234.2022.2079348

Kostić AŽ, Dojčinović B, Špirović Trifunović B, Milinčić DD, Nedić N, Stanojević S, Pešić M. Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment. in Journal of Environmental Science and Health, Part B. 2022;57(7):568-575.
doi:10.1080/03601234.2022.2079348 .

Kostić, Aleksandar Ž., Dojčinović, Biljana, Špirović Trifunović, Bojana, Milinčić, Danijel D., Nedić, Nebojša, Stanojević, Sladjana, Pešić, Mirjana, "Micro/trace/toxic elements and insecticide residues level in monofloral bee-collected sunflower pollen- health risk assessment" in Journal of Environmental Science and Health, Part B, 57, no. 7 (2022):568-575,
https://doi.org/10.1080/03601234.2022.2079348 . .

TY  - JOUR
AU  - Stojanović, Sanja
AU  - Stepanović, Jelena
AU  - Špirović Trifunović, Bojana
AU  - Duduk, Nataša
AU  - Dojnov, Biljana
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5585
AB  - Research background. Inulinases are used for fructooligosaccharide production and they are of interest for both scientific community and industry. Black aspergilli represent a diverse group of species that has use for enzyme production, in particular some species are known as potent inulinase producers. Finding new potential producers from the environment is as important as improving the production with known strains. Safe use of enzymes produced by aspergilli in food industry is placed ahead of their benefit for inulinase production.  Experimental approach. Here we show a specific approach to finding/screening of newly isolated fungal inulinase producers that combines a newly developed screening method and an equally important assessment of the toxigenic potential of the fungus. In this study 39 black aspergilli collected from different substrates in Serbia were identified and assessed for inulinase production.  Results and conclusions. The most common species were Aspergillus tubingensis (51.2 %), followed by A. niger (23.1 %), A. welwitschiae (23.1 %) and A. uvarum (2.6 %). The isolates for inulinase production were selected using a cheap and easy, fast and non-hazardous alternative inulinase screening test developed in this work. Enzymatic activity of selected inulinase-producing strains was confirmed spectrophotometrically. Since some A. niger and A. welwitschiae strains are able to produce mycotoxins ochratoxin A (OTA) and fumonisins (FB), the toxigenic potential of selected inulinase producers was assessed analytically and genetically. Fungal enzyme producer can be considered safe for use in food industry only after comparing the results of both approaches for investigating toxic potential, the direct presence of mycotoxins in the enzyme preparation (analytically) and the presence of mycotoxin gene clusters (genetically). In some strains the absence of OTA and FB production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters, respectively. The two best inulinase producers and mycotoxin non-producers (without mycotoxin production capability as additional safety) were selected as potential candidates for further development of enzyme production.  Novelty and scientific contribution. The presented innovative approach for the selection of potential fungal enzyme producer shows that only non-toxigenic fungi could be considered as useful in food industry. Although this study was done on local isolates, the approach is applicable globally.
PB  - Faculty of Food Technology and Biotechnology - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing
VL  - 60
IS  - 4
SP  - 421
EP  - 433
DO  - 10.17113/ftb.60.04.22.7521
ER  - 

@article{
author = "Stojanović, Sanja and Stepanović, Jelena and Špirović Trifunović, Bojana and Duduk, Nataša and Dojnov, Biljana and Duduk, Bojan and Vujčić, Zoran",
year = "2022",
abstract = "Research background. Inulinases are used for fructooligosaccharide production and they are of interest for both scientific community and industry. Black aspergilli represent a diverse group of species that has use for enzyme production, in particular some species are known as potent inulinase producers. Finding new potential producers from the environment is as important as improving the production with known strains. Safe use of enzymes produced by aspergilli in food industry is placed ahead of their benefit for inulinase production.  Experimental approach. Here we show a specific approach to finding/screening of newly isolated fungal inulinase producers that combines a newly developed screening method and an equally important assessment of the toxigenic potential of the fungus. In this study 39 black aspergilli collected from different substrates in Serbia were identified and assessed for inulinase production.  Results and conclusions. The most common species were Aspergillus tubingensis (51.2 %), followed by A. niger (23.1 %), A. welwitschiae (23.1 %) and A. uvarum (2.6 %). The isolates for inulinase production were selected using a cheap and easy, fast and non-hazardous alternative inulinase screening test developed in this work. Enzymatic activity of selected inulinase-producing strains was confirmed spectrophotometrically. Since some A. niger and A. welwitschiae strains are able to produce mycotoxins ochratoxin A (OTA) and fumonisins (FB), the toxigenic potential of selected inulinase producers was assessed analytically and genetically. Fungal enzyme producer can be considered safe for use in food industry only after comparing the results of both approaches for investigating toxic potential, the direct presence of mycotoxins in the enzyme preparation (analytically) and the presence of mycotoxin gene clusters (genetically). In some strains the absence of OTA and FB production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters, respectively. The two best inulinase producers and mycotoxin non-producers (without mycotoxin production capability as additional safety) were selected as potential candidates for further development of enzyme production.  Novelty and scientific contribution. The presented innovative approach for the selection of potential fungal enzyme producer shows that only non-toxigenic fungi could be considered as useful in food industry. Although this study was done on local isolates, the approach is applicable globally.",
publisher = "Faculty of Food Technology and Biotechnology - University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing",
volume = "60",
number = "4",
pages = "421-433",
doi = "10.17113/ftb.60.04.22.7521"
}

Stojanović, S., Stepanović, J., Špirović Trifunović, B., Duduk, N., Dojnov, B., Duduk, B.,& Vujčić, Z.. (2022). Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing. in Food Technology and Biotechnology
Faculty of Food Technology and Biotechnology - University of Zagreb., 60(4), 421-433.
https://doi.org/10.17113/ftb.60.04.22.7521

Stojanović S, Stepanović J, Špirović Trifunović B, Duduk N, Dojnov B, Duduk B, Vujčić Z. Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing. in Food Technology and Biotechnology. 2022;60(4):421-433.
doi:10.17113/ftb.60.04.22.7521 .

Stojanović, Sanja, Stepanović, Jelena, Špirović Trifunović, Bojana, Duduk, Nataša, Dojnov, Biljana, Duduk, Bojan, Vujčić, Zoran, "Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing" in Food Technology and Biotechnology, 60, no. 4 (2022):421-433,
https://doi.org/10.17113/ftb.60.04.22.7521 . .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Stepanović, Jelena
AU  - Margetić, Aleksandra
AU  - Duduk, Nataša
AU  - Dojnov, Biljana
AU  - Vujčić, Zoran
AU  - Duduk, Bojan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5816
AB  - Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.
PB  - Sociedade Portuguesa de Química
C3  - Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line
T1  - Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate
SP  - 172
UR  - https://hdl.handle.net/21.15107/rcub_cer_5816
ER  - 

@conference{
author = "Stojanović, Sanja and Stepanović, Jelena and Margetić, Aleksandra and Duduk, Nataša and Dojnov, Biljana and Vujčić, Zoran and Duduk, Bojan",
year = "2021",
abstract = "Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.",
publisher = "Sociedade Portuguesa de Química",
journal = "Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line",
title = "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate",
pages = "172",
url = "https://hdl.handle.net/21.15107/rcub_cer_5816"
}

Stojanović, S., Stepanović, J., Margetić, A., Duduk, N., Dojnov, B., Vujčić, Z.,& Duduk, B.. (2021). Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line
Sociedade Portuguesa de Química., 172.
https://hdl.handle.net/21.15107/rcub_cer_5816

Stojanović S, Stepanović J, Margetić A, Duduk N, Dojnov B, Vujčić Z, Duduk B. Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line. 2021;:172.
https://hdl.handle.net/21.15107/rcub_cer_5816 .

Stojanović, Sanja, Stepanović, Jelena, Margetić, Aleksandra, Duduk, Nataša, Dojnov, Biljana, Vujčić, Zoran, Duduk, Bojan, "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate" in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line (2021):172,
https://hdl.handle.net/21.15107/rcub_cer_5816 .