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Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique
dc.creator | Pejić, Nataša | |
dc.creator | Maksimović, Jelena | |
dc.creator | Blagojević, Slavica | |
dc.creator | Anić, Slobodan | |
dc.creator | Čupić, Željko | |
dc.creator | Kolar-Anić, Ljiljana | |
dc.date.accessioned | 2019-01-30T17:33:10Z | |
dc.date.available | 2019-01-30T17:33:10Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0103-5053 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/1130 | |
dc.description.abstract | Simple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO3 and H2SO4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift Delta E-m, and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 x 10(-5)-2.68 x 10(-4) mol L-1 and 2.98 x 10(-5)-3.58 x 10(-4) mol L-1, respectively. The methods have an excellent sample throughput of 30 samples h(-1) (method A) and 7 samples h(-1) (method B) with the sensitivity determined to be 1.1 x 10(-5) mol L-1 (method A) and 8.9 x 10(-6) mol L-1 (method B) as well as the precision RSD LT = 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail. | en |
dc.publisher | Sociedade Brasileira de Química | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172015/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45001/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Journal of the Brazilian Chemical Society | |
dc.subject | uric acid | en |
dc.subject | perturbation technique | en |
dc.subject | Bray-Liebhafsky oscillatory reaction | en |
dc.subject | Dushman reaction | en |
dc.subject | urine | en |
dc.title | Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique | en |
dc.type | article | |
dc.rights.license | BY | |
dcterms.abstract | Максимовиц, Јелена П.; Пејиц, Натаса Д.; Aниц, Слободан Р.; Благојевиц, Славица М.; Колар-Aниц, Љиљана З.; Чупић, Жељко; | |
dc.citation.volume | 23 | |
dc.citation.issue | 8 | |
dc.citation.spage | 1450 | |
dc.citation.epage | 1459 | |
dc.citation.other | 23(8): 1450-1459 | |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_cer_1130 | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs/bitstream/id/14452/v23n8a07.pdf | |
dc.identifier.scopus | 2-s2.0-84866410166 | |
dc.identifier.wos | 000309162200007 | |
dc.type.version | publishedVersion |