dc.creator | Ristović, Marina | |
dc.creator | Stojanović, Sanja | |
dc.creator | Margetić, Aleksandra | |
dc.creator | Vujčić, Zoran | |
dc.creator | Dojnov, Biljana | |
dc.date.accessioned | 2023-02-26T17:36:59Z | |
dc.date.available | 2023-02-26T17:36:59Z | |
dc.date.issued | 2021 | |
dc.identifier.isbn | 978-86-7220-108-6 (FOC) | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/5818 | |
dc.description.abstract | Wild-type microorganisms from the environment represent a wide source of potential
enzyme producers. In order to determine whether an isolated microorganism produces an
enzyme of interest, various screen tests have been developed. A new screening method
for detection of endo and exo-xylanase activity including short time growth of fungal
strains on a minimal medium containing xylan (inducible substrate) as a carbon source is
developed and used for testing 58 fungal isolates from genus Aspergillus. The test is based
on the diffusion of samples (fermentation extracts) in polyacrylamide gel incorporated by
xylan. Endoxylanase activity is detected as enlightenment in the gel after staining of xylan
with Congo Red. Exoxylanase activity was visualized as a precipitate after staining of
reduction oligosaccharide ends with NBT. Selected isolate A. tubigensis was grown on
SSF where corn cob served as an inducible substrate. In order to examine the influence of
nitrogen sources on endoxylanase production and fungal growth, two sources (peptone and
urea) were varied in 3 concentrations (1, 5 and 10 g/L). There were statistically significant
differences in the obtained activities. The increase in activity compared to the screening
medium was ~250 times. The obtained enzymes with high specific activity were further
used for the production of xylooligosaccharides in high yield which showed that the
selection of strain A. tubingensis was good. | sr |
dc.language.iso | en | sr |
dc.publisher | University of Belgrade - Faculty of Chemistry | sr |
dc.publisher | Serbian Biochemical Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia | sr |
dc.subject | screen test | sr |
dc.subject | detection | sr |
dc.subject | activity | sr |
dc.subject | xylan | sr |
dc.subject | Endoxylanase activity | sr |
dc.subject | Exoxylanase activity | sr |
dc.title | Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production | sr |
dc.type | conferenceObject | sr |
dc.rights.license | BY | sr |
dc.citation.spage | 138 | |
dc.citation.epage | 139 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_cer_5818 | |
dc.identifier.fulltext | http://cer.ihtm.bg.ac.rs/bitstream/id/24148/bitstream_24148.pdf | |
dc.type.version | publishedVersion | sr |