dc.creator | Franich, Andjela | |
dc.creator | Đorđević, Ivana S. | |
dc.creator | Živković, Marija D. | |
dc.creator | Rajković, Snežana | |
dc.creator | Janjić, Goran | |
dc.creator | Đuran, Miloš | |
dc.date.accessioned | 2021-11-04T10:21:44Z | |
dc.date.available | 2021-11-04T10:21:44Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1432-1327 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/4832 | |
dc.description.abstract | The mechanism of action of most approved drugs in use today is based on their binding to specific proteins or DNA. One
of the achievements of this research is a new perspective for recognition of binding modes to DNA by monitoring of
changes in measured and stoichiometric values of absorbance at 260 nm. UV–Vis and IR spectroscopy, gel electrophoresis
and docking study were used for investigation of binding properties of three dinuclear platinum(II) complexes containing
different pyridine-based bridging ligands, [{Pt(en)Cl}2(μ-4,4’-bipy)]Cl2・2H2O (Pt1), [{Pt(en)Cl}2(μ-bpa)]Cl2・4H2O (Pt2)
and [{Pt(en)Cl}2(μ-bpe)]Cl2・4H2O (Pt3) to DNA (4,4’-bipy, bpa and bpe are 4,4′-bipyridine, 1,2-bis(4-pyridyl)ethane and
1,2-bis(4-pyridyl)ethene, respectively). In contrast to the system with well-known intercalated ligand (EtBr), covalently bound
ligand (cis-Pt) and with minor groove binder (Hoechst 33258), which do not have significant differences in measured and
stoichiometric values, the most pronounced deviations are recorded for two dinuclear platinum(II) complexes (Pt1 and Pt2),
as a consequence of complex binding to the phosphate backbone and bending of DNA helix. The hydrolysis of complexes
and changes in DNA conformation were also analysed as phenomena that may have an impact on the changes in absorbance. | sr |
dc.language.iso | en | sr |
dc.publisher | Springer | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200122/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200111/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Journal of Biological Inorganic Chemistry | sr |
dc.subject | DNA binding modes | sr |
dc.subject | Dinuclear Pt(II) complex | sr |
dc.subject | Pyridine-based bridging ligands | sr |
dc.subject | Quantum–mechanical calculations | sr |
dc.subject | UV–vis spectroscopy | sr |
dc.title | Dinuclear platinum(II) complexes as the pattern for phosphate backbone binding: a new perspective for recognition of binding modes to DNA | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.citation.volume | 27 | |
dc.citation.spage | 65 | |
dc.citation.epage | 79 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 34714401 | |
dc.identifier.doi | 10.1007/s00775-021-01911-6 | |
dc.identifier.scopus | 2-s2.0-85118271990 | |
dc.identifier.wos | 000712719900002 | |
dc.type.version | publishedVersion | sr |