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Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects
dc.creator | Adžić, Miroslav | |
dc.creator | Nićiforović, Ana | |
dc.creator | Zarić, Božidarka | |
dc.creator | Nešković-Konstantinović, Zora | |
dc.creator | Spasić, Snežana | |
dc.creator | Jones, David | |
dc.creator | Radojčić, Marija | |
dc.date.accessioned | 2020-12-02T15:00:35Z | |
dc.date.available | 2020-12-02T15:00:35Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1351-0002 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/3945 | |
dc.description.abstract | To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour. | en |
dc.language.iso | en | sr |
dc.publisher | Taylor & Francis | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/143042/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Redox Report | sr |
dc.subject | ionising radiation | sr |
dc.subject | leukocytes | sr |
dc.subject | breast cancer | sr |
dc.subject | superoxide dismutase | sr |
dc.subject | catalase | sr |
dc.title | Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects | en |
dc.type | article | sr |
dc.rights.license | BY | sr |
dcterms.abstract | Нићифоровић, Aна; Спасић, Снежана; Јонес, Давид; Нешковић-Константиновић, Зора; Радојчић, Марија; Зарић, Божидарка; Aджић, Мирослав; | |
dc.citation.volume | 13 | |
dc.citation.issue | 1 | |
dc.citation.spage | 17 | |
dc.citation.epage | 22 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 18284847 | |
dc.identifier.doi | 10.1179/135100008X259088 | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs/bitstream/id/18219/bitstream_18219.pdf | |
dc.identifier.scopus | 2-s2.0-41149143150 | |
dc.identifier.wos | 000257499600003 | |
dc.type.version | publishedVersion | sr |