TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica
AU  - Spasić, Snežana
AU  - Jones, David R.
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/738
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Taylor & Francis Group
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
VL  - 15
IS  - 1
SP  - 29
EP  - 35
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica and Spasić, Snežana and Jones, David R. and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Taylor & Francis Group",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
volume = "15",
number = "1",
pages = "29-35",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z., Spasić, S., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Taylor & Francis Group., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević Z, Spasić S, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica, Spasić, Snežana, Jones, David R., Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3937
AB  - The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
VL  - 344
IS  - 1
SP  - 80
EP  - 84
DO  - 10.1016/j.carres.2008.09.025
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasić, Snežana and Jones, David and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
volume = "344",
number = "1",
pages = "80-84",
doi = "10.1016/j.carres.2008.09.025"
}

Spasojević, I., Mojović, M., Blagojević, D., Spasić, S., Jones, D., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025

Spasojević I, Mojović M, Blagojević D, Spasić S, Jones D, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .

Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasić, Snežana, Jones, David, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

TY  - JOUR
AU  - Oreščanin, Zorana
AU  - Milovanović, Slobodan R.
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Spasić, Mihajlo
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3938
AB  - The conversion of nitric oxide (NO
) into its congeners nitrosonium (NO ) and nitroxyl (HNO/NO) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert
NO into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and
presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced
relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO-mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not
solely endothelium-derived but involves changes in vascular smooth muscl
PB  - Springer
T2  - Pharmacological Report
T1  - Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners
VL  - 59
SP  - 315
EP  - 322
UR  - https://hdl.handle.net/21.15107/rcub_cer_3938
ER  - 

@article{
author = "Oreščanin, Zorana and Milovanović, Slobodan R. and Spasić, Snežana and Jones, David and Spasić, Mihajlo",
year = "2007",
abstract = "The conversion of nitric oxide (NO
) into its congeners nitrosonium (NO ) and nitroxyl (HNO/NO) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert
NO into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and
presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced
relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO-mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not
solely endothelium-derived but involves changes in vascular smooth muscl",
publisher = "Springer",
journal = "Pharmacological Report",
title = "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners",
volume = "59",
pages = "315-322",
url = "https://hdl.handle.net/21.15107/rcub_cer_3938"
}

Oreščanin, Z., Milovanović, S. R., Spasić, S., Jones, D.,& Spasić, M.. (2007). Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Report
Springer., 59, 315-322.
https://hdl.handle.net/21.15107/rcub_cer_3938

Oreščanin Z, Milovanović SR, Spasić S, Jones D, Spasić M. Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Report. 2007;59:315-322.
https://hdl.handle.net/21.15107/rcub_cer_3938 .

Oreščanin, Zorana, Milovanović, Slobodan R., Spasić, Snežana, Jones, David, Spasić, Mihajlo, "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners" in Pharmacological Report, 59 (2007):315-322,
https://hdl.handle.net/21.15107/rcub_cer_3938 .