Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
2010
Аутори
Spasojević, IvanMojović, Miloš
Stević, Zorica
Spasić, Snežana
Jones, David R.
Morina, Arian
Spasić, Mihajlo
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing cat...alytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
Кључне речи:
copper / iron / Fenton reaction / cerebrospinal fluid / diethyldithiocarbamateИзвор:
Redox Report, 2010, 15, 1, 29-35Издавач:
- Taylor & Francis Group
Финансирање / пројекти:
- Улога редокс активних супстанци у процесима одржавања хомеостазе живих система (RS-MESTD-MPN2006-2010-143034)
- Биофизичка истраживања мембранских процеса: интеракција мембранских рецептора и канала са спољашњим факторима и интрацелуларна регулација (RS-MESTD-MPN2006-2010-143016)
DOI: 10.1179/174329210X12650506623087
ISSN: 1351-0002
PubMed: 20196926
WoS: 000275303600004
Scopus: 2-s2.0-77949463742
Институција/група
IHTMTY - JOUR AU - Spasojević, Ivan AU - Mojović, Miloš AU - Stević, Zorica AU - Spasić, Snežana AU - Jones, David R. AU - Morina, Arian AU - Spasić, Mihajlo PY - 2010 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/738 AB - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings. PB - Taylor & Francis Group T2 - Redox Report T1 - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid VL - 15 IS - 1 SP - 29 EP - 35 DO - 10.1179/174329210X12650506623087 ER -
@article{ author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica and Spasić, Snežana and Jones, David R. and Morina, Arian and Spasić, Mihajlo", year = "2010", abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.", publisher = "Taylor & Francis Group", journal = "Redox Report", title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid", volume = "15", number = "1", pages = "29-35", doi = "10.1179/174329210X12650506623087" }
Spasojević, I., Mojović, M., Stević, Z., Spasić, S., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report Taylor & Francis Group., 15(1), 29-35. https://doi.org/10.1179/174329210X12650506623087
Spasojević I, Mojović M, Stević Z, Spasić S, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35. doi:10.1179/174329210X12650506623087 .
Spasojević, Ivan, Mojović, Miloš, Stević, Zorica, Spasić, Snežana, Jones, David R., Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35, https://doi.org/10.1179/174329210X12650506623087 . .