TY  - JOUR
AU  - Stojadinović, Sanja
AU  - Jovančićević, Branimir
AU  - Šajnović, Aleksandra
AU  - Golumbenau, Mariana
AU  - Almasan, Roxana
AU  - Jovanović, Đorđe
AU  - Brceski, Ilija
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4006
AB  - The Techirghiol Lake, located on the Romanian coast of the Black Sea, is a hypersaline lake. In this environment, a unique ecosystem in Europe has developed with the specific capacity of producing mud by the decomposition of flora and fauna under the microbial activity (saprogenic sludge), which is used for therapeutic purposes.
In this study, hydrocarbon compositions of the Techirghiol Lake mud are investigated in order to determine the origin and type of organic matter (OM). All samples are characterized by a high abundance of n-alkanes, short and high chain alkenes, pimarane, phyllocladane, indicating that OM was mainly derived from phytoplankton, filamentous green alga Cladophora vagabunda, Cyanobacteria, submerged/floating macrophytes, terrestrial and emergent plants. The terrestrial origin of organic matter is probably related to a significant input by wind or rivers. Typical oil distributions of terpanes and steranes biomarkers clearly indicate that the muds of Techirghiol Lake, in addition to native organic matter, contain a relatively low contribution of petroleum type pollutants, as well as PAH compounds of pyrogenic origin. 
The relatively high abundance of 1-chloroalkenes, which in some samples represent the most abundant compounds in the total distribution of hydrocarbons, together with isoprenoid thiophenes indicate the local presence of significant amounts of chloride and sulphur species in the water column or surface sediment.
PB  - Parlar Scientific Publications
T2  - Fresenius Environmental Bulletin
T1  - Organic-geochemical characteristics of the mud from the Techirghiol Lake, Romania
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4898
ER  - 

@article{
author = "Stojadinović, Sanja and Jovančićević, Branimir and Šajnović, Aleksandra and Golumbenau, Mariana and Almasan, Roxana and Jovanović, Đorđe and Brceski, Ilija",
year = "2021",
abstract = "The Techirghiol Lake, located on the Romanian coast of the Black Sea, is a hypersaline lake. In this environment, a unique ecosystem in Europe has developed with the specific capacity of producing mud by the decomposition of flora and fauna under the microbial activity (saprogenic sludge), which is used for therapeutic purposes.
In this study, hydrocarbon compositions of the Techirghiol Lake mud are investigated in order to determine the origin and type of organic matter (OM). All samples are characterized by a high abundance of n-alkanes, short and high chain alkenes, pimarane, phyllocladane, indicating that OM was mainly derived from phytoplankton, filamentous green alga Cladophora vagabunda, Cyanobacteria, submerged/floating macrophytes, terrestrial and emergent plants. The terrestrial origin of organic matter is probably related to a significant input by wind or rivers. Typical oil distributions of terpanes and steranes biomarkers clearly indicate that the muds of Techirghiol Lake, in addition to native organic matter, contain a relatively low contribution of petroleum type pollutants, as well as PAH compounds of pyrogenic origin. 
The relatively high abundance of 1-chloroalkenes, which in some samples represent the most abundant compounds in the total distribution of hydrocarbons, together with isoprenoid thiophenes indicate the local presence of significant amounts of chloride and sulphur species in the water column or surface sediment.",
publisher = "Parlar Scientific Publications",
journal = "Fresenius Environmental Bulletin",
title = "Organic-geochemical characteristics of the mud from the Techirghiol Lake, Romania",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4898"
}

Stojadinović, S., Jovančićević, B., Šajnović, A., Golumbenau, M., Almasan, R., Jovanović, Đ.,& Brceski, I.. (2021). Organic-geochemical characteristics of the mud from the Techirghiol Lake, Romania. in Fresenius Environmental Bulletin
Parlar Scientific Publications..
https://hdl.handle.net/21.15107/rcub_cherry_4898

Stojadinović S, Jovančićević B, Šajnović A, Golumbenau M, Almasan R, Jovanović Đ, Brceski I. Organic-geochemical characteristics of the mud from the Techirghiol Lake, Romania. in Fresenius Environmental Bulletin. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4898 .

Stojadinović, Sanja, Jovančićević, Branimir, Šajnović, Aleksandra, Golumbenau, Mariana, Almasan, Roxana, Jovanović, Đorđe, Brceski, Ilija, "Organic-geochemical characteristics of the mud from the Techirghiol Lake, Romania" in Fresenius Environmental Bulletin (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4898 .

TY  - JOUR
AU  - Čolić, Slavica D.
AU  - Bakić, Ivana V.
AU  - Dabić Zagorac, Dragana
AU  - Natić, Maja
AU  - Smailagić, Anita T.
AU  - Pergal, Marija
AU  - Pešić, Mirjana B.
AU  - Milinčić, Danijel D.
AU  - Rabrenović, Biljana B.
AU  - Fotirić Akšić, Milica M.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4013
AB  - The study was designed to investigate chemical composition and kernel quality of almond cultivars ‘Troito’, ‘Marcona’ and ‘Texas’ grafted on Myrobalan plum seedlings and ‘GF 677’ rootstock under drought stress. Oil content varied from 53.34% (‘Marcona’) to 58.11% (‘Texas’), and protein content was in a range from 17.36% (‘Texas’) to 23.02% (‘Troito’). ‘GF 677’ contributed to a higher content of oleic and lower content of linoleic acid during favourable conditions but had the opposite trend during drought. Regarding flavanols, the most abundant in all three cultivars was epigalocatechin, followed by catechin. From the results it is concluded that scions on ‘GF 677’ were better adapted to water stress conditions in comparison with Myrobalan plum seedlings as they had significantly higher kernel weight, kernel ratio, and yield per tree. Also, kernel quality attributes, contents of proteins, oleic acid, and oleic acid/linoleic acid ratio were higher, while the content of amandin decreased. Furthermore, an increase of flavanols (especially epigallocatechin gallate) on ‘GF 677’ could have led to the enhancement of drought tolerance. Besides the fact that water deficit decreased the majority of chemical components in almond kernels, the grafting combination ‘Texas’/’GF 677’ can be grown in drought conditions without significant loss of kernel quality.
PB  - Elsevier
T2  - Scientia Horticulturae
T1  - Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition
VL  - 275
SP  - 109705
DO  - 10.1016/j.scienta.2020.109705
ER  - 

@article{
author = "Čolić, Slavica D. and Bakić, Ivana V. and Dabić Zagorac, Dragana and Natić, Maja and Smailagić, Anita T. and Pergal, Marija and Pešić, Mirjana B. and Milinčić, Danijel D. and Rabrenović, Biljana B. and Fotirić Akšić, Milica M.",
year = "2021",
abstract = "The study was designed to investigate chemical composition and kernel quality of almond cultivars ‘Troito’, ‘Marcona’ and ‘Texas’ grafted on Myrobalan plum seedlings and ‘GF 677’ rootstock under drought stress. Oil content varied from 53.34% (‘Marcona’) to 58.11% (‘Texas’), and protein content was in a range from 17.36% (‘Texas’) to 23.02% (‘Troito’). ‘GF 677’ contributed to a higher content of oleic and lower content of linoleic acid during favourable conditions but had the opposite trend during drought. Regarding flavanols, the most abundant in all three cultivars was epigalocatechin, followed by catechin. From the results it is concluded that scions on ‘GF 677’ were better adapted to water stress conditions in comparison with Myrobalan plum seedlings as they had significantly higher kernel weight, kernel ratio, and yield per tree. Also, kernel quality attributes, contents of proteins, oleic acid, and oleic acid/linoleic acid ratio were higher, while the content of amandin decreased. Furthermore, an increase of flavanols (especially epigallocatechin gallate) on ‘GF 677’ could have led to the enhancement of drought tolerance. Besides the fact that water deficit decreased the majority of chemical components in almond kernels, the grafting combination ‘Texas’/’GF 677’ can be grown in drought conditions without significant loss of kernel quality.",
publisher = "Elsevier",
journal = "Scientia Horticulturae",
title = "Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition",
volume = "275",
pages = "109705",
doi = "10.1016/j.scienta.2020.109705"
}

Čolić, S. D., Bakić, I. V., Dabić Zagorac, D., Natić, M., Smailagić, A. T., Pergal, M., Pešić, M. B., Milinčić, D. D., Rabrenović, B. B.,& Fotirić Akšić, M. M.. (2021). Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. in Scientia Horticulturae
Elsevier., 275, 109705.
https://doi.org/10.1016/j.scienta.2020.109705

Čolić SD, Bakić IV, Dabić Zagorac D, Natić M, Smailagić AT, Pergal M, Pešić MB, Milinčić DD, Rabrenović BB, Fotirić Akšić MM. Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition. in Scientia Horticulturae. 2021;275:109705.
doi:10.1016/j.scienta.2020.109705 .

Čolić, Slavica D., Bakić, Ivana V., Dabić Zagorac, Dragana, Natić, Maja, Smailagić, Anita T., Pergal, Marija, Pešić, Mirjana B., Milinčić, Danijel D., Rabrenović, Biljana B., Fotirić Akšić, Milica M., "Chemical Fingerprint and Kernel Quality Assessment in Different Grafting Combinations of Almond Under Stress Condition" in Scientia Horticulturae, 275 (2021):109705,
https://doi.org/10.1016/j.scienta.2020.109705 . .

TY  - JOUR
AU  - Anđelković, Ljubica
AU  - Jeremić, Dejan
AU  - Milenković, Milica R.
AU  - Radosavljević, Jelena
AU  - Vulić, Predrag
AU  - Pavlović, Vladimir B.
AU  - Manojlović, Dragan
AU  - Nikolić, Aleksandar S.
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3145
AB  - A simple organic-phase synthesis process was used to produce bare NiFe2O4 and ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 ferrite nanoparticles. X-ray powder diffractograms for all investigated powders show characteristic peaks of a spinel cubic structure without a secondary phase. Transmission electron microscopy (TEM) indicated the presence of nanoparticles that are smaller than 20 nm. The release of divalent ions (Ni2+ and Zn2+) from synthesized nanoparticles that were dispersed in saline solution, phosphate-buffered saline (PBS) and human serum, as determined by the inductively coupled plasma mass spectrometry (ICP-MS) method, was lower than 2 wt %. These results demonstrate the stability of the investigated nanoparticles in biologically relevant media and exclude the toxicity of Ni2+ and Zn2+ due to metal ion release, thereby opening a broad range of (bio)medical applications.
PB  - Elsevier
T2  - Ceramics International
T1  - Synthesis, characterization and in vitro evaluation of divalent ion release from stable NiFe2O4, ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 nanoparticles
VL  - 46
IS  - 3
SP  - 3528
EP  - 3533
DO  - 10.1016/j.ceramint.2019.10.068
ER  - 

@article{
author = "Anđelković, Ljubica and Jeremić, Dejan and Milenković, Milica R. and Radosavljević, Jelena and Vulić, Predrag and Pavlović, Vladimir B. and Manojlović, Dragan and Nikolić, Aleksandar S.",
year = "2020",
abstract = "A simple organic-phase synthesis process was used to produce bare NiFe2O4 and ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 ferrite nanoparticles. X-ray powder diffractograms for all investigated powders show characteristic peaks of a spinel cubic structure without a secondary phase. Transmission electron microscopy (TEM) indicated the presence of nanoparticles that are smaller than 20 nm. The release of divalent ions (Ni2+ and Zn2+) from synthesized nanoparticles that were dispersed in saline solution, phosphate-buffered saline (PBS) and human serum, as determined by the inductively coupled plasma mass spectrometry (ICP-MS) method, was lower than 2 wt %. These results demonstrate the stability of the investigated nanoparticles in biologically relevant media and exclude the toxicity of Ni2+ and Zn2+ due to metal ion release, thereby opening a broad range of (bio)medical applications.",
publisher = "Elsevier",
journal = "Ceramics International",
title = "Synthesis, characterization and in vitro evaluation of divalent ion release from stable NiFe2O4, ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 nanoparticles",
volume = "46",
number = "3",
pages = "3528-3533",
doi = "10.1016/j.ceramint.2019.10.068"
}

Anđelković, L., Jeremić, D., Milenković, M. R., Radosavljević, J., Vulić, P., Pavlović, V. B., Manojlović, D.,& Nikolić, A. S.. (2020). Synthesis, characterization and in vitro evaluation of divalent ion release from stable NiFe2O4, ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 nanoparticles. in Ceramics International
Elsevier., 46(3), 3528-3533.
https://doi.org/10.1016/j.ceramint.2019.10.068

Anđelković L, Jeremić D, Milenković MR, Radosavljević J, Vulić P, Pavlović VB, Manojlović D, Nikolić AS. Synthesis, characterization and in vitro evaluation of divalent ion release from stable NiFe2O4, ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 nanoparticles. in Ceramics International. 2020;46(3):3528-3533.
doi:10.1016/j.ceramint.2019.10.068 .

Anđelković, Ljubica, Jeremić, Dejan, Milenković, Milica R., Radosavljević, Jelena, Vulić, Predrag, Pavlović, Vladimir B., Manojlović, Dragan, Nikolić, Aleksandar S., "Synthesis, characterization and in vitro evaluation of divalent ion release from stable NiFe2O4, ZnFe2O4 and core-shell ZnFe2O4@NiFe2O4 nanoparticles" in Ceramics International, 46, no. 3 (2020):3528-3533,
https://doi.org/10.1016/j.ceramint.2019.10.068 . .

TY  - CONF
AU  - Smailagić, Anita
AU  - Veljović, Sonja
AU  - Dojčinović, Biljana
AU  - Natić, Maja
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7544
AB  - In the production of some alcoholic beverages one of the most important practices is theageing process in a presence of wood. This process contributes to improved sensorycharacteristics such as aroma, color, taste and astringency. Although oak heartwood isthe most used material in cooperage, other species such as chestnut, cherry, and mulberrycan be also considered. Currently, limited published data are available on the mineralcontent of the wood extract from cooperage industry, although some research was doneto investigate the content of heavy metals in alcoholic beverages1, especially in wine2.Daily consumption of the wine and brandy with an elevated content of heavy metalsmight cause chronical poisoning. To avoid this issue, the maximum allowableconcentration (MAC, mg L–1) in fruit brandies were established for lead, zinc, arsenium,and copper.1The aim of this research was to investigate elemental composition of ethanolic extracts,obtained from alternative wood species used in Balkan cooperage, such as mulberry(Morus alba L.), Myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robiniapseudoacacia L.), and wild cherry (Prunus avium (L.) L.). Elements were determined byinductively coupled plasma-optical emission spectroscopy (ICP-OES). Results indicatedthat the elemental composition of wild cherry extract was much lower than in other nonoakwood extracts, and all investigated wood extracts have lower content of traceelements than maximum allowable concentration in fruit brandies.
C3  - Seventh Conference of the Young Chemists of Serbia Book of Abstracts
T1  - Poster: "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage"
UR  - https://hdl.handle.net/21.15107/rcub_cer_7544
ER  - 

@conference{
author = "Smailagić, Anita and Veljović, Sonja and Dojčinović, Biljana and Natić, Maja",
year = "2019",
abstract = "In the production of some alcoholic beverages one of the most important practices is theageing process in a presence of wood. This process contributes to improved sensorycharacteristics such as aroma, color, taste and astringency. Although oak heartwood isthe most used material in cooperage, other species such as chestnut, cherry, and mulberrycan be also considered. Currently, limited published data are available on the mineralcontent of the wood extract from cooperage industry, although some research was doneto investigate the content of heavy metals in alcoholic beverages1, especially in wine2.Daily consumption of the wine and brandy with an elevated content of heavy metalsmight cause chronical poisoning. To avoid this issue, the maximum allowableconcentration (MAC, mg L–1) in fruit brandies were established for lead, zinc, arsenium,and copper.1The aim of this research was to investigate elemental composition of ethanolic extracts,obtained from alternative wood species used in Balkan cooperage, such as mulberry(Morus alba L.), Myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robiniapseudoacacia L.), and wild cherry (Prunus avium (L.) L.). Elements were determined byinductively coupled plasma-optical emission spectroscopy (ICP-OES). Results indicatedthat the elemental composition of wild cherry extract was much lower than in other nonoakwood extracts, and all investigated wood extracts have lower content of traceelements than maximum allowable concentration in fruit brandies.",
journal = "Seventh Conference of the Young Chemists of Serbia Book of Abstracts",
title = "Poster: "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage"",
url = "https://hdl.handle.net/21.15107/rcub_cer_7544"
}

Smailagić, A., Veljović, S., Dojčinović, B.,& Natić, M.. (2019). Poster: "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage". in Seventh Conference of the Young Chemists of Serbia Book of Abstracts.
https://hdl.handle.net/21.15107/rcub_cer_7544

Smailagić A, Veljović S, Dojčinović B, Natić M. Poster: "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage". in Seventh Conference of the Young Chemists of Serbia Book of Abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_cer_7544 .

Smailagić, Anita, Veljović, Sonja, Dojčinović, Biljana, Natić, Maja, "Poster: "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage"" in Seventh Conference of the Young Chemists of Serbia Book of Abstracts (2019),
https://hdl.handle.net/21.15107/rcub_cer_7544 .

TY  - CONF
AU  - Smailagić, Anita
AU  - Veljović, Sonja
AU  - Dojčinović, Biljana
AU  - Natić, Maja
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7543
AB  - In the production of some alcoholic beverages one of the most important practices is theageing process in a presence of wood. This process contributes to improved sensorycharacteristics such as aroma, color, taste and astringency. Although oak heartwood isthe most used material in cooperage, other species such as chestnut, cherry, and mulberrycan be also considered. Currently, limited published data are available on the mineralcontent of the wood extract from cooperage industry, although some research was doneto investigate the content of heavy metals in alcoholic beverages1, especially in wine2.Daily consumption of the wine and brandy with an elevated content of heavy metalsmight cause chronical poisoning. To avoid this issue, the maximum allowableconcentration (MAC, mg L–1) in fruit brandies were established for lead, zinc, arsenium,and copper.1The aim of this research was to investigate elemental composition of ethanolic extracts,obtained from alternative wood species used in Balkan cooperage, such as mulberry(Morus alba L.), Myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robiniapseudoacacia L.), and wild cherry (Prunus avium (L.) L.). Elements were determined byinductively coupled plasma-optical emission spectroscopy (ICP-OES). Results indicatedthat the elemental composition of wild cherry extract was much lower than in other nonoakwood extracts, and all investigated wood extracts have lower content of traceelements than maximum allowable concentration in fruit brandies.
C3  - Seventh Conference of the Young Chemists of Serbia Book of Abstracts
T1  - Elemental composition of non-oak wood extracts commonly used in Balkan cooperage
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5297
ER  - 

@conference{
author = "Smailagić, Anita and Veljović, Sonja and Dojčinović, Biljana and Natić, Maja",
year = "2019",
abstract = "In the production of some alcoholic beverages one of the most important practices is theageing process in a presence of wood. This process contributes to improved sensorycharacteristics such as aroma, color, taste and astringency. Although oak heartwood isthe most used material in cooperage, other species such as chestnut, cherry, and mulberrycan be also considered. Currently, limited published data are available on the mineralcontent of the wood extract from cooperage industry, although some research was doneto investigate the content of heavy metals in alcoholic beverages1, especially in wine2.Daily consumption of the wine and brandy with an elevated content of heavy metalsmight cause chronical poisoning. To avoid this issue, the maximum allowableconcentration (MAC, mg L–1) in fruit brandies were established for lead, zinc, arsenium,and copper.1The aim of this research was to investigate elemental composition of ethanolic extracts,obtained from alternative wood species used in Balkan cooperage, such as mulberry(Morus alba L.), Myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robiniapseudoacacia L.), and wild cherry (Prunus avium (L.) L.). Elements were determined byinductively coupled plasma-optical emission spectroscopy (ICP-OES). Results indicatedthat the elemental composition of wild cherry extract was much lower than in other nonoakwood extracts, and all investigated wood extracts have lower content of traceelements than maximum allowable concentration in fruit brandies.",
journal = "Seventh Conference of the Young Chemists of Serbia Book of Abstracts",
title = "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5297"
}

Smailagić, A., Veljović, S., Dojčinović, B.,& Natić, M.. (2019). Elemental composition of non-oak wood extracts commonly used in Balkan cooperage. in Seventh Conference of the Young Chemists of Serbia Book of Abstracts, 58-58.
https://hdl.handle.net/21.15107/rcub_cherry_5297

Smailagić A, Veljović S, Dojčinović B, Natić M. Elemental composition of non-oak wood extracts commonly used in Balkan cooperage. in Seventh Conference of the Young Chemists of Serbia Book of Abstracts. 2019;:58-58.
https://hdl.handle.net/21.15107/rcub_cherry_5297 .

Smailagić, Anita, Veljović, Sonja, Dojčinović, Biljana, Natić, Maja, "Elemental composition of non-oak wood extracts commonly used in Balkan cooperage" in Seventh Conference of the Young Chemists of Serbia Book of Abstracts (2019):58-58,
https://hdl.handle.net/21.15107/rcub_cherry_5297 .

TY  - JOUR
AU  - Lješević, Marija
AU  - Gojgić-Cvijović, Gordana
AU  - Stanimirović, Bojana
AU  - Beškoski, Vladimir
AU  - Brčeski, Ilija
PY  - 2019
UR  - http://www.jepe-journal.info/journal-content/vol-20-no-3
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3947
AB  - Microorganisms can grow on the surface of concrete and inside its pores and microcracks, producing different metabolites. Microbial metabolites, particularly acids, degrade concrete components and enhance its deterioration by abiotic factors. Deterioration of concrete is a serious problem worldwide since it affects construction functionality and requires high maintenance costs.
This paper presents microbiological and chemical analyses of 12 concrete samples originating from
6 hydroelectric power plants in Serbia, investigated in order to evaluate the key chemical factors
affecting microbial growth on concrete. In most of the concrete samples, microorganisms from all
examined groups were present in high numbers (bacteria 8.64 × 103–3.4 × 108, fungi 9 × 102–2.08
× 106, sulphur-oxidising bacteria 16.8–2.5×104 CFU/g). The high number and the presence of various
physiological groups of microorganisms indicate the high intensity of deterioration caused by
biological sources. Values of pH of the concrete samples were in the range 8.46–11.23, Ca content
5.43–19.93%, Fe 151–61100 ppm, sulphate 37.4–623.7 ppm and chloride 96.3–914.1 ppm. Correlation analysis between microbiological and chemical factors indicated a statistically significant strong negative correlation between sulphur-oxidising bacteria and pH (–0.759, p < 0.01).
PB  - Balkan Environmental Association
T2  - Journal of Environmental Protection and Ecology
T1  - Microbially-induced deterioration of concrete from hydroelectric power plants – an initial study
VL  - 20
IS  - 3
SP  - 1180
EP  - 1188
UR  - https://hdl.handle.net/21.15107/rcub_cer_3947
ER  - 

@article{
author = "Lješević, Marija and Gojgić-Cvijović, Gordana and Stanimirović, Bojana and Beškoski, Vladimir and Brčeski, Ilija",
year = "2019",
abstract = "Microorganisms can grow on the surface of concrete and inside its pores and microcracks, producing different metabolites. Microbial metabolites, particularly acids, degrade concrete components and enhance its deterioration by abiotic factors. Deterioration of concrete is a serious problem worldwide since it affects construction functionality and requires high maintenance costs.
This paper presents microbiological and chemical analyses of 12 concrete samples originating from
6 hydroelectric power plants in Serbia, investigated in order to evaluate the key chemical factors
affecting microbial growth on concrete. In most of the concrete samples, microorganisms from all
examined groups were present in high numbers (bacteria 8.64 × 103–3.4 × 108, fungi 9 × 102–2.08
× 106, sulphur-oxidising bacteria 16.8–2.5×104 CFU/g). The high number and the presence of various
physiological groups of microorganisms indicate the high intensity of deterioration caused by
biological sources. Values of pH of the concrete samples were in the range 8.46–11.23, Ca content
5.43–19.93%, Fe 151–61100 ppm, sulphate 37.4–623.7 ppm and chloride 96.3–914.1 ppm. Correlation analysis between microbiological and chemical factors indicated a statistically significant strong negative correlation between sulphur-oxidising bacteria and pH (–0.759, p < 0.01).",
publisher = "Balkan Environmental Association",
journal = "Journal of Environmental Protection and Ecology",
title = "Microbially-induced deterioration of concrete from hydroelectric power plants – an initial study",
volume = "20",
number = "3",
pages = "1180-1188",
url = "https://hdl.handle.net/21.15107/rcub_cer_3947"
}

Lješević, M., Gojgić-Cvijović, G., Stanimirović, B., Beškoski, V.,& Brčeski, I.. (2019). Microbially-induced deterioration of concrete from hydroelectric power plants – an initial study. in Journal of Environmental Protection and Ecology
Balkan Environmental Association., 20(3), 1180-1188.
https://hdl.handle.net/21.15107/rcub_cer_3947

Lješević M, Gojgić-Cvijović G, Stanimirović B, Beškoski V, Brčeski I. Microbially-induced deterioration of concrete from hydroelectric power plants – an initial study. in Journal of Environmental Protection and Ecology. 2019;20(3):1180-1188.
https://hdl.handle.net/21.15107/rcub_cer_3947 .

Lješević, Marija, Gojgić-Cvijović, Gordana, Stanimirović, Bojana, Beškoski, Vladimir, Brčeski, Ilija, "Microbially-induced deterioration of concrete from hydroelectric power plants – an initial study" in Journal of Environmental Protection and Ecology, 20, no. 3 (2019):1180-1188,
https://hdl.handle.net/21.15107/rcub_cer_3947 .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3205
AB  - Among widely used chromatographic methods modern thin-layer chromatography is not only the
simplest to perform but is also considered as respectable analytical method in various phases of
drug discovery and development processes such as monitoring of synthesis, identification of bioactive
substances from various natural sources and their isolation and purification, determination
of lipophilicity and other physico-chemical parameters, quantitative structure-activity relationship
studies, bioautography, as well as qualitative and quantitative analysis of drugs and their metabolites. An overview of recently published papers dealing with application of thin-layer chromatography in medicinal chemistry is presented.
PB  - Taylor & Francis Inc.
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Thin-layer chromatography in medicinal chemistry
VL  - 42
IS  - 9-10
SP  - 238
EP  - 248
DO  - 10.1080/10826076.2019.1585615
ER  - 

@article{
author = "Šegan, Sandra and Opsenica, Dejan and Milojković-Opsenica, Dušanka",
year = "2019",
abstract = "Among widely used chromatographic methods modern thin-layer chromatography is not only the
simplest to perform but is also considered as respectable analytical method in various phases of
drug discovery and development processes such as monitoring of synthesis, identification of bioactive
substances from various natural sources and their isolation and purification, determination
of lipophilicity and other physico-chemical parameters, quantitative structure-activity relationship
studies, bioautography, as well as qualitative and quantitative analysis of drugs and their metabolites. An overview of recently published papers dealing with application of thin-layer chromatography in medicinal chemistry is presented.",
publisher = "Taylor & Francis Inc.",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Thin-layer chromatography in medicinal chemistry",
volume = "42",
number = "9-10",
pages = "238-248",
doi = "10.1080/10826076.2019.1585615"
}

Šegan, S., Opsenica, D.,& Milojković-Opsenica, D.. (2019). Thin-layer chromatography in medicinal chemistry. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc.., 42(9-10), 238-248.
https://doi.org/10.1080/10826076.2019.1585615

Šegan S, Opsenica D, Milojković-Opsenica D. Thin-layer chromatography in medicinal chemistry. in Journal of Liquid Chromatography & Related Technologies. 2019;42(9-10):238-248.
doi:10.1080/10826076.2019.1585615 .

Šegan, Sandra, Opsenica, Dejan, Milojković-Opsenica, Dušanka, "Thin-layer chromatography in medicinal chemistry" in Journal of Liquid Chromatography & Related Technologies, 42, no. 9-10 (2019):238-248,
https://doi.org/10.1080/10826076.2019.1585615 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Čomić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2932
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3139
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
VL  - 222
SP  - 148
EP  - 158
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Čomić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojković-Opsenica, Dušanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
volume = "222",
pages = "148-158",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Čomić, L. R., Vasiljević, B., Opsenica, D., Milojković-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Čomić LR, Vasiljević B, Opsenica D, Milojković-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Čomić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojković-Opsenica, Dušanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Kašanin-Grubin, Milica
AU  - Šajnović, Aleksandra
AU  - Perunović, Tamara
AU  - Stojanović, Ksenija
AU  - Simić, Vladimir
AU  - Gajica, Gordana
AU  - Brčeski, Ilija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3906
AB  - The scale of human impacts on the natural environment is now considerably larger than at any point in history. The concept of geomorphic sensitivity can help to understand the rate, magnitude and nature of landscape adjustment to perturbation in a given natural system. Aim of this research is to show that geochemical and mineralogical data are important factors in determining landscape sensitivity. To test the suggested premise Neogene lacustrine Kremna basin (Serbia) was selected since sediments found in lacustrine basins are usually prone to dispersion and erosion. Furthermore, lacustrine basins often bear fossil and mineral resources and because of that are often undergoing land use changes. For the purpose of this study, samples of serpentinite, carbonates, marly carbonates, oil shale and tuff were analyzed. Besides mineralogical and petrographic analyses, samples were subjected to the weathering experiments. Obtained results indicate that marly carbonates and tuff are most prone to dispersion primarily due to presence of clay minerals. However, oil shale which also contains clay minerals showed minor leaching characteristics due to high content of organic matter. It can be concluded that mineralogical and geochemical characteristics are important for determining landscape sensitivity to erosion processes of an area.
PB  - Romania : Editura Universităţii din Oradea
T2  - Natural Resources and Sustainable Development
T1  - Assessment of landscape sensitivity based on geochemical characteristics of sediments (Kremna basin)
VL  - 8
IS  - 2
SP  - 148
EP  - 165
DO  - 10.31924/nrsd.v8i2.016
ER  - 

@article{
author = "Kašanin-Grubin, Milica and Šajnović, Aleksandra and Perunović, Tamara and Stojanović, Ksenija and Simić, Vladimir and Gajica, Gordana and Brčeski, Ilija",
year = "2018",
abstract = "The scale of human impacts on the natural environment is now considerably larger than at any point in history. The concept of geomorphic sensitivity can help to understand the rate, magnitude and nature of landscape adjustment to perturbation in a given natural system. Aim of this research is to show that geochemical and mineralogical data are important factors in determining landscape sensitivity. To test the suggested premise Neogene lacustrine Kremna basin (Serbia) was selected since sediments found in lacustrine basins are usually prone to dispersion and erosion. Furthermore, lacustrine basins often bear fossil and mineral resources and because of that are often undergoing land use changes. For the purpose of this study, samples of serpentinite, carbonates, marly carbonates, oil shale and tuff were analyzed. Besides mineralogical and petrographic analyses, samples were subjected to the weathering experiments. Obtained results indicate that marly carbonates and tuff are most prone to dispersion primarily due to presence of clay minerals. However, oil shale which also contains clay minerals showed minor leaching characteristics due to high content of organic matter. It can be concluded that mineralogical and geochemical characteristics are important for determining landscape sensitivity to erosion processes of an area.",
publisher = "Romania : Editura Universităţii din Oradea",
journal = "Natural Resources and Sustainable Development",
title = "Assessment of landscape sensitivity based on geochemical characteristics of sediments (Kremna basin)",
volume = "8",
number = "2",
pages = "148-165",
doi = "10.31924/nrsd.v8i2.016"
}

Kašanin-Grubin, M., Šajnović, A., Perunović, T., Stojanović, K., Simić, V., Gajica, G.,& Brčeski, I.. (2018). Assessment of landscape sensitivity based on geochemical characteristics of sediments (Kremna basin). in Natural Resources and Sustainable Development
Romania : Editura Universităţii din Oradea., 8(2), 148-165.
https://doi.org/10.31924/nrsd.v8i2.016

Kašanin-Grubin M, Šajnović A, Perunović T, Stojanović K, Simić V, Gajica G, Brčeski I. Assessment of landscape sensitivity based on geochemical characteristics of sediments (Kremna basin). in Natural Resources and Sustainable Development. 2018;8(2):148-165.
doi:10.31924/nrsd.v8i2.016 .

Kašanin-Grubin, Milica, Šajnović, Aleksandra, Perunović, Tamara, Stojanović, Ksenija, Simić, Vladimir, Gajica, Gordana, Brčeski, Ilija, "Assessment of landscape sensitivity based on geochemical characteristics of sediments (Kremna basin)" in Natural Resources and Sustainable Development, 8, no. 2 (2018):148-165,
https://doi.org/10.31924/nrsd.v8i2.016 . .

TY  - JOUR
AU  - Jeremić, Dejan
AU  - Dordevic, Milena
AU  - Miletić, Srđan
AU  - Anđelković, Ljubica
AU  - Sladić, Dušan
AU  - Brčeski, Ilija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2436
AB  - In this work, three novel silver(I) complexes with an almost completely rigid and bulky monodentate ligand, 1-adamantanamine, were synthesized. The aliphatic amine, 1-adamantanamine, is the sole electron donor ligand in these complexes. In addition to spectroscopic characterization, the basic biological activities of the new compounds were investigated and their minimum inhibitory concentrations were determined. The antifungal and antibacterial activities indicate that these compounds could potentially be applied as new therapeutics.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Novel silver(I) compounds with 1-adamantanamine
VL  - 83
IS  - 6
SP  - 699
EP  - 705
DO  - 10.2298/JSC171114041J
ER  - 

@article{
author = "Jeremić, Dejan and Dordevic, Milena and Miletić, Srđan and Anđelković, Ljubica and Sladić, Dušan and Brčeski, Ilija",
year = "2018",
abstract = "In this work, three novel silver(I) complexes with an almost completely rigid and bulky monodentate ligand, 1-adamantanamine, were synthesized. The aliphatic amine, 1-adamantanamine, is the sole electron donor ligand in these complexes. In addition to spectroscopic characterization, the basic biological activities of the new compounds were investigated and their minimum inhibitory concentrations were determined. The antifungal and antibacterial activities indicate that these compounds could potentially be applied as new therapeutics.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Novel silver(I) compounds with 1-adamantanamine",
volume = "83",
number = "6",
pages = "699-705",
doi = "10.2298/JSC171114041J"
}

Jeremić, D., Dordevic, M., Miletić, S., Anđelković, L., Sladić, D.,& Brčeski, I.. (2018). Novel silver(I) compounds with 1-adamantanamine. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 83(6), 699-705.
https://doi.org/10.2298/JSC171114041J

Jeremić D, Dordevic M, Miletić S, Anđelković L, Sladić D, Brčeski I. Novel silver(I) compounds with 1-adamantanamine. in Journal of the Serbian Chemical Society. 2018;83(6):699-705.
doi:10.2298/JSC171114041J .

Jeremić, Dejan, Dordevic, Milena, Miletić, Srđan, Anđelković, Ljubica, Sladić, Dušan, Brčeski, Ilija, "Novel silver(I) compounds with 1-adamantanamine" in Journal of the Serbian Chemical Society, 83, no. 6 (2018):699-705,
https://doi.org/10.2298/JSC171114041J . .

TY  - JOUR
AU  - Milojković-Opsenica, Dušanka
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Trifković, Jelena
AU  - Tešić, Živoslav
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2475
AB  - The methods of correlating molecular structure of substances expressed as descriptors, to their biological activity are commonly denoted as Quantitative Structure-Activity Relationships (QSARs). This concept, typically applied in drug discovery processes, is also widely used for correlation of molecular structure and physicochemical properties of solutes in so-called quantitative structure-property relationship (QSPR) studies, as well as for explanation of chromatographic behavior, i.e., separation mechanisms of analytes, where is termed as quantitative structure-retention relationship (QSRR). Mathematical expressions of structural characteristics of substances, named as molecular descriptors, can be calculated by various computational techniques or experimentally determined by different analytical methods. Thin-layer chromatography as a rapid, sensitive, and economical liquid chromatographic method has been widely used for determination of various chromatographic descriptors applicable in QSAR/QSPR studies. An overview of recently published papers dealing with this concept is presented. [GRAPHICS] .
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Thin-layer chromatography in quantitative structure-activity relationship studies
VL  - 41
IS  - 6
SP  - 272
EP  - 281
DO  - 10.1080/10826076.2018.1447892
ER  - 

@article{
author = "Milojković-Opsenica, Dušanka and Andrić, Filip and Šegan, Sandra and Trifković, Jelena and Tešić, Živoslav",
year = "2018",
abstract = "The methods of correlating molecular structure of substances expressed as descriptors, to their biological activity are commonly denoted as Quantitative Structure-Activity Relationships (QSARs). This concept, typically applied in drug discovery processes, is also widely used for correlation of molecular structure and physicochemical properties of solutes in so-called quantitative structure-property relationship (QSPR) studies, as well as for explanation of chromatographic behavior, i.e., separation mechanisms of analytes, where is termed as quantitative structure-retention relationship (QSRR). Mathematical expressions of structural characteristics of substances, named as molecular descriptors, can be calculated by various computational techniques or experimentally determined by different analytical methods. Thin-layer chromatography as a rapid, sensitive, and economical liquid chromatographic method has been widely used for determination of various chromatographic descriptors applicable in QSAR/QSPR studies. An overview of recently published papers dealing with this concept is presented. [GRAPHICS] .",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Thin-layer chromatography in quantitative structure-activity relationship studies",
volume = "41",
number = "6",
pages = "272-281",
doi = "10.1080/10826076.2018.1447892"
}

Milojković-Opsenica, D., Andrić, F., Šegan, S., Trifković, J.,& Tešić, Ž.. (2018). Thin-layer chromatography in quantitative structure-activity relationship studies. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 41(6), 272-281.
https://doi.org/10.1080/10826076.2018.1447892

Milojković-Opsenica D, Andrić F, Šegan S, Trifković J, Tešić Ž. Thin-layer chromatography in quantitative structure-activity relationship studies. in Journal of Liquid Chromatography & Related Technologies. 2018;41(6):272-281.
doi:10.1080/10826076.2018.1447892 .

Milojković-Opsenica, Dušanka, Andrić, Filip, Šegan, Sandra, Trifković, Jelena, Tešić, Živoslav, "Thin-layer chromatography in quantitative structure-activity relationship studies" in Journal of Liquid Chromatography & Related Technologies, 41, no. 6 (2018):272-281,
https://doi.org/10.1080/10826076.2018.1447892 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojevic, Petar
AU  - Pavić, Aleksandar
AU  - Radojevic, Ivana
AU  - Čomić, Ljiljana R.
AU  - Vasiljevic, Branka
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
AU  - Senerovic, Lidija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2310
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
VL  - 222
SP  - 148
EP  - 158
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojevic, Petar and Pavić, Aleksandar and Radojevic, Ivana and Čomić, Ljiljana R. and Vasiljevic, Branka and Opsenica, Dejan and Milojković-Opsenica, Dušanka and Senerovic, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
volume = "222",
pages = "148-158",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojevic, P., Pavić, A., Radojevic, I., Čomić, L. R., Vasiljevic, B., Opsenica, D., Milojković-Opsenica, D.,& Senerovic, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojevic P, Pavić A, Radojevic I, Čomić LR, Vasiljevic B, Opsenica D, Milojković-Opsenica D, Senerovic L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojevic, Petar, Pavić, Aleksandar, Radojevic, Ivana, Čomić, Ljiljana R., Vasiljevic, Branka, Opsenica, Dejan, Milojković-Opsenica, Dušanka, Senerovic, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Pantelić, Milica
AU  - Dabić Zagorac, Dragana
AU  - Ćirić, Ivanka Z.
AU  - Pergal, Marija
AU  - Relić, Dubravka
AU  - Todić, Slavica R.
AU  - Natić, Maja
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2059
AB  - This research was designed to determine phenolics content, radical scavenging activity and mineral composition of 22 grapevine leaves of diverse varietal origin. Samples were collected from two vineyards located in Central Serbia (Belgrade vineyard area) and Eastern Serbia (Mlava vineyard area). Leaf extracts were characterized mostly with phenolics acids, flavonols and flavan-3-ols. Ellagic acid and rutin were the most abundant components, found in the concentration up to 770 mg/kg and 450 mg/kg of dry weight, respectively. Total phenolic content was in the range from 27.5 to 76.0 g GAE/kg of dry weight while the radical scavenging activity ranged from 0.429 to 0.867 mmol TE/kg of dry weight. The most common element in all samples was IC (content ranging from 2.30 g/kg to 6.77 g/kg of dry weight), followed by Ca, Na, and B. A variation in the chemical composition was shown to be convenient way to differentiate among the grape leaves of diverse varietal origin. Clear separation was demonstrated between objects of different geographical origin by Principal Component Analysis. Serbian variety 'Petra', an interspecies hybrid with atypical composition, was detected as an outlier in comparison to other samples.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia
VL  - 62
SP  - 76
EP  - 83
DO  - 10.1016/j.jfca.2017.05.002
ER  - 

@article{
author = "Pantelić, Milica and Dabić Zagorac, Dragana and Ćirić, Ivanka Z. and Pergal, Marija and Relić, Dubravka and Todić, Slavica R. and Natić, Maja",
year = "2017",
abstract = "This research was designed to determine phenolics content, radical scavenging activity and mineral composition of 22 grapevine leaves of diverse varietal origin. Samples were collected from two vineyards located in Central Serbia (Belgrade vineyard area) and Eastern Serbia (Mlava vineyard area). Leaf extracts were characterized mostly with phenolics acids, flavonols and flavan-3-ols. Ellagic acid and rutin were the most abundant components, found in the concentration up to 770 mg/kg and 450 mg/kg of dry weight, respectively. Total phenolic content was in the range from 27.5 to 76.0 g GAE/kg of dry weight while the radical scavenging activity ranged from 0.429 to 0.867 mmol TE/kg of dry weight. The most common element in all samples was IC (content ranging from 2.30 g/kg to 6.77 g/kg of dry weight), followed by Ca, Na, and B. A variation in the chemical composition was shown to be convenient way to differentiate among the grape leaves of diverse varietal origin. Clear separation was demonstrated between objects of different geographical origin by Principal Component Analysis. Serbian variety 'Petra', an interspecies hybrid with atypical composition, was detected as an outlier in comparison to other samples.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia",
volume = "62",
pages = "76-83",
doi = "10.1016/j.jfca.2017.05.002"
}

Pantelić, M., Dabić Zagorac, D., Ćirić, I. Z., Pergal, M., Relić, D., Todić, S. R.,& Natić, M.. (2017). Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 62, 76-83.
https://doi.org/10.1016/j.jfca.2017.05.002

Pantelić M, Dabić Zagorac D, Ćirić IZ, Pergal M, Relić D, Todić SR, Natić M. Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia. in Journal of Food Composition and Analysis. 2017;62:76-83.
doi:10.1016/j.jfca.2017.05.002 .

Pantelić, Milica, Dabić Zagorac, Dragana, Ćirić, Ivanka Z., Pergal, Marija, Relić, Dubravka, Todić, Slavica R., Natić, Maja, "Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia" in Journal of Food Composition and Analysis, 62 (2017):76-83,
https://doi.org/10.1016/j.jfca.2017.05.002 . .

TY  - JOUR
AU  - Pantelić, Milica
AU  - Dabić Zagorac, Dragana
AU  - Ćirić, Ivanka Z.
AU  - Pergal, Marija
AU  - Relić, Dubravka
AU  - Todić, Slavica R.
AU  - Natić, Maja
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2921
AB  - This research was designed to determine phenolics content, radical scavenging activity and mineral composition of 22 grapevine leaves of diverse varietal origin. Samples were collected from two vineyards located in Central Serbia (Belgrade vineyard area) and Eastern Serbia (Mlava vineyard area). Leaf extracts were characterized mostly with phenolics acids, flavonols and flavan-3-ols. Ellagic acid and rutin were the most abundant components, found in the concentration up to 770 mg/kg and 450 mg/kg of dry weight, respectively. Total phenolic content was in the range from 27.5 to 76.0 g GAE/kg of dry weight while the radical scavenging activity ranged from 0.429 to 0.867 mmol TE/kg of dry weight. The most common element in all samples was IC (content ranging from 2.30 g/kg to 6.77 g/kg of dry weight), followed by Ca, Na, and B. A variation in the chemical composition was shown to be convenient way to differentiate among the grape leaves of diverse varietal origin. Clear separation was demonstrated between objects of different geographical origin by Principal Component Analysis. Serbian variety 'Petra', an interspecies hybrid with atypical composition, was detected as an outlier in comparison to other samples.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition and Analysis
T1  - Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia
VL  - 62
SP  - 76
EP  - 83
DO  - 10.1016/j.jfca.2017.05.002
ER  - 

@article{
author = "Pantelić, Milica and Dabić Zagorac, Dragana and Ćirić, Ivanka Z. and Pergal, Marija and Relić, Dubravka and Todić, Slavica R. and Natić, Maja",
year = "2017",
abstract = "This research was designed to determine phenolics content, radical scavenging activity and mineral composition of 22 grapevine leaves of diverse varietal origin. Samples were collected from two vineyards located in Central Serbia (Belgrade vineyard area) and Eastern Serbia (Mlava vineyard area). Leaf extracts were characterized mostly with phenolics acids, flavonols and flavan-3-ols. Ellagic acid and rutin were the most abundant components, found in the concentration up to 770 mg/kg and 450 mg/kg of dry weight, respectively. Total phenolic content was in the range from 27.5 to 76.0 g GAE/kg of dry weight while the radical scavenging activity ranged from 0.429 to 0.867 mmol TE/kg of dry weight. The most common element in all samples was IC (content ranging from 2.30 g/kg to 6.77 g/kg of dry weight), followed by Ca, Na, and B. A variation in the chemical composition was shown to be convenient way to differentiate among the grape leaves of diverse varietal origin. Clear separation was demonstrated between objects of different geographical origin by Principal Component Analysis. Serbian variety 'Petra', an interspecies hybrid with atypical composition, was detected as an outlier in comparison to other samples.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition and Analysis",
title = "Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia",
volume = "62",
pages = "76-83",
doi = "10.1016/j.jfca.2017.05.002"
}

Pantelić, M., Dabić Zagorac, D., Ćirić, I. Z., Pergal, M., Relić, D., Todić, S. R.,& Natić, M.. (2017). Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia. in Journal of Food Composition and Analysis
Academic Press Inc Elsevier Science, San Diego., 62, 76-83.
https://doi.org/10.1016/j.jfca.2017.05.002

Pantelić M, Dabić Zagorac D, Ćirić IZ, Pergal M, Relić D, Todić SR, Natić M. Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia. in Journal of Food Composition and Analysis. 2017;62:76-83.
doi:10.1016/j.jfca.2017.05.002 .

Pantelić, Milica, Dabić Zagorac, Dragana, Ćirić, Ivanka Z., Pergal, Marija, Relić, Dubravka, Todić, Slavica R., Natić, Maja, "Phenolic profiles, antioxidant activity and minerals in leaves of different grapevine varieties grown in Serbia" in Journal of Food Composition and Analysis, 62 (2017):76-83,
https://doi.org/10.1016/j.jfca.2017.05.002 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Bozinovic, Nina
AU  - Opsenica, Igor
AU  - Andrić, Filip
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2243
AB  - Lipophilicity is one of the essential properties influencing drug absorption, excretion and metabolism. It is used for screening viable drug candidates. Chromatographic behavior of thiepino[3,2-c: 6,7-c'] dipyridine and 16 benzothiepino[3,2-c] pyridine derivatives as potential antifungal drugs was studied using thin-layer chromatography under typical reversed-phase conditions and two microemulsion chromatographic systems. Seventeen chromatographic and nine in silico lipophilicity measures were estimated. They were compared by classical multivariate approaches: principal component analysis, hierarchical cluster analysis, and ranked and grouped by the non-parametricmethod-Sum of ranking differences. Two computational and two chromatographic descriptors from the typical reversed-phase conditions using acetone/ water mixtures emerged as the best candidates for lipophilicity estimation. The principal component scores related to typical reversed-phase conditions using dioxane/ water were ranked as statistically insignificant ( the worst). Microemulsion systems were positioned in between, performing worse than in silico estimates. Thiepine derivatives were ranked and grouped by sum of ranking differences, fusing multiple lipophilicity measures. In multicriteria maximization ranking, the compound substituted by phenyl group at position 8 was selected as the most lipophilic one. It is also the most active against Candida albicans. The ranking confirmed that introduction of phenyl core is essential for increasing the lipophilicity of the studied compounds.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Separation Science
T1  - Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs
VL  - 40
IS  - 10
SP  - 2089
EP  - 2096
DO  - 10.1002/jssc.201601442
ER  - 

@article{
author = "Šegan, Sandra and Bozinovic, Nina and Opsenica, Igor and Andrić, Filip",
year = "2017",
abstract = "Lipophilicity is one of the essential properties influencing drug absorption, excretion and metabolism. It is used for screening viable drug candidates. Chromatographic behavior of thiepino[3,2-c: 6,7-c'] dipyridine and 16 benzothiepino[3,2-c] pyridine derivatives as potential antifungal drugs was studied using thin-layer chromatography under typical reversed-phase conditions and two microemulsion chromatographic systems. Seventeen chromatographic and nine in silico lipophilicity measures were estimated. They were compared by classical multivariate approaches: principal component analysis, hierarchical cluster analysis, and ranked and grouped by the non-parametricmethod-Sum of ranking differences. Two computational and two chromatographic descriptors from the typical reversed-phase conditions using acetone/ water mixtures emerged as the best candidates for lipophilicity estimation. The principal component scores related to typical reversed-phase conditions using dioxane/ water were ranked as statistically insignificant ( the worst). Microemulsion systems were positioned in between, performing worse than in silico estimates. Thiepine derivatives were ranked and grouped by sum of ranking differences, fusing multiple lipophilicity measures. In multicriteria maximization ranking, the compound substituted by phenyl group at position 8 was selected as the most lipophilic one. It is also the most active against Candida albicans. The ranking confirmed that introduction of phenyl core is essential for increasing the lipophilicity of the studied compounds.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Separation Science",
title = "Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs",
volume = "40",
number = "10",
pages = "2089-2096",
doi = "10.1002/jssc.201601442"
}

Šegan, S., Bozinovic, N., Opsenica, I.,& Andrić, F.. (2017). Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs. in Journal of Separation Science
Wiley-V C H Verlag Gmbh, Weinheim., 40(10), 2089-2096.
https://doi.org/10.1002/jssc.201601442

Šegan S, Bozinovic N, Opsenica I, Andrić F. Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs. in Journal of Separation Science. 2017;40(10):2089-2096.
doi:10.1002/jssc.201601442 .

Šegan, Sandra, Bozinovic, Nina, Opsenica, Igor, Andrić, Filip, "Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs" in Journal of Separation Science, 40, no. 10 (2017):2089-2096,
https://doi.org/10.1002/jssc.201601442 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Bozinovic, Nina
AU  - Opsenica, Igor
AU  - Andrić, Filip
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2982
AB  - Lipophilicity is one of the essential properties influencing drug absorption, excretion and metabolism. It is used for screening viable drug candidates. Chromatographic behavior of thiepino[3,2-c: 6,7-c'] dipyridine and 16 benzothiepino[3,2-c] pyridine derivatives as potential antifungal drugs was studied using thin-layer chromatography under typical reversed-phase conditions and two microemulsion chromatographic systems. Seventeen chromatographic and nine in silico lipophilicity measures were estimated. They were compared by classical multivariate approaches: principal component analysis, hierarchical cluster analysis, and ranked and grouped by the non-parametricmethod-Sum of ranking differences. Two computational and two chromatographic descriptors from the typical reversed-phase conditions using acetone/ water mixtures emerged as the best candidates for lipophilicity estimation. The principal component scores related to typical reversed-phase conditions using dioxane/ water were ranked as statistically insignificant ( the worst). Microemulsion systems were positioned in between, performing worse than in silico estimates. Thiepine derivatives were ranked and grouped by sum of ranking differences, fusing multiple lipophilicity measures. In multicriteria maximization ranking, the compound substituted by phenyl group at position 8 was selected as the most lipophilic one. It is also the most active against Candida albicans. The ranking confirmed that introduction of phenyl core is essential for increasing the lipophilicity of the studied compounds.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Journal of Separation Science
T1  - Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs
VL  - 40
IS  - 10
SP  - 2089
EP  - 2096
DO  - 10.1002/jssc.201601442
ER  - 

@article{
author = "Šegan, Sandra and Bozinovic, Nina and Opsenica, Igor and Andrić, Filip",
year = "2017",
abstract = "Lipophilicity is one of the essential properties influencing drug absorption, excretion and metabolism. It is used for screening viable drug candidates. Chromatographic behavior of thiepino[3,2-c: 6,7-c'] dipyridine and 16 benzothiepino[3,2-c] pyridine derivatives as potential antifungal drugs was studied using thin-layer chromatography under typical reversed-phase conditions and two microemulsion chromatographic systems. Seventeen chromatographic and nine in silico lipophilicity measures were estimated. They were compared by classical multivariate approaches: principal component analysis, hierarchical cluster analysis, and ranked and grouped by the non-parametricmethod-Sum of ranking differences. Two computational and two chromatographic descriptors from the typical reversed-phase conditions using acetone/ water mixtures emerged as the best candidates for lipophilicity estimation. The principal component scores related to typical reversed-phase conditions using dioxane/ water were ranked as statistically insignificant ( the worst). Microemulsion systems were positioned in between, performing worse than in silico estimates. Thiepine derivatives were ranked and grouped by sum of ranking differences, fusing multiple lipophilicity measures. In multicriteria maximization ranking, the compound substituted by phenyl group at position 8 was selected as the most lipophilic one. It is also the most active against Candida albicans. The ranking confirmed that introduction of phenyl core is essential for increasing the lipophilicity of the studied compounds.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Journal of Separation Science",
title = "Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs",
volume = "40",
number = "10",
pages = "2089-2096",
doi = "10.1002/jssc.201601442"
}

Šegan, S., Bozinovic, N., Opsenica, I.,& Andrić, F.. (2017). Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs. in Journal of Separation Science
Wiley-V C H Verlag Gmbh, Weinheim., 40(10), 2089-2096.
https://doi.org/10.1002/jssc.201601442

Šegan S, Bozinovic N, Opsenica I, Andrić F. Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs. in Journal of Separation Science. 2017;40(10):2089-2096.
doi:10.1002/jssc.201601442 .

Šegan, Sandra, Bozinovic, Nina, Opsenica, Igor, Andrić, Filip, "Consensus-based comparison of chromatographic and computationally estimated lipophilicity of benzothiepino[3,2-c]pyridine derivatives as potential antifungal drugs" in Journal of Separation Science, 40, no. 10 (2017):2089-2096,
https://doi.org/10.1002/jssc.201601442 . .

TY  - JOUR
AU  - Vukojević, Vesna
AU  - Trifković, Jelena
AU  - Krgovic, Rada
AU  - Milojković-Opsenica, Dušanka
AU  - Marković, Marijana
AU  - Amaizah, Naser Ramdan R.
AU  - Mutić, Jelena
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1913
AB  - Fourteen metals and metalloids were determined in Conyza canadensis L. harvested from the fly ash landfill of the thermoelectric power plant "Kolubara" (Serbia). Fly ash samples were collected together with the plant samples and subjected to sequential extraction according to the three-step sequential extraction scheme proposed by the Community Bureau of Reference (BCR; now the Standards, Measurements and Testing Program). The contents of metals and metalloids were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) in plant root and the aboveground part and correlated with their contents in the fly ash samples. The bioconcentration factor (BCF) and translocation factors (TF) were calculated to access uptake of metals from fly ash and their translocation to the aboveground part. Results regarding As revealed that fly ash samples in the proximity of the active cassette had higher amounts of the element. Principal component analysis (PCA) showed that As had no impact on the classification of plant parts. BCF for As ranged from 1.44 to 23.8 and varied, depending on the investigated area; TF for As ranged from 0.43 to 2.61, indicating that the plant translocated As from root to shoot. In addition to As, Conyza canadensis L. exhibited efficient uptake of other metals from fly ash. According to the calculated BCF and TF, the plant retained Al, Fe and Cr in the root and translocated Zn, Cd, Cu and As from root to shoot in the course of the detoxifying process.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of Biological Sciences
T1  - Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill
VL  - 68
IS  - 4
SP  - 829
EP  - 835
DO  - 10.2298/ABS151011071V
ER  - 

@article{
author = "Vukojević, Vesna and Trifković, Jelena and Krgovic, Rada and Milojković-Opsenica, Dušanka and Marković, Marijana and Amaizah, Naser Ramdan R. and Mutić, Jelena",
year = "2016",
abstract = "Fourteen metals and metalloids were determined in Conyza canadensis L. harvested from the fly ash landfill of the thermoelectric power plant "Kolubara" (Serbia). Fly ash samples were collected together with the plant samples and subjected to sequential extraction according to the three-step sequential extraction scheme proposed by the Community Bureau of Reference (BCR; now the Standards, Measurements and Testing Program). The contents of metals and metalloids were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) in plant root and the aboveground part and correlated with their contents in the fly ash samples. The bioconcentration factor (BCF) and translocation factors (TF) were calculated to access uptake of metals from fly ash and their translocation to the aboveground part. Results regarding As revealed that fly ash samples in the proximity of the active cassette had higher amounts of the element. Principal component analysis (PCA) showed that As had no impact on the classification of plant parts. BCF for As ranged from 1.44 to 23.8 and varied, depending on the investigated area; TF for As ranged from 0.43 to 2.61, indicating that the plant translocated As from root to shoot. In addition to As, Conyza canadensis L. exhibited efficient uptake of other metals from fly ash. According to the calculated BCF and TF, the plant retained Al, Fe and Cr in the root and translocated Zn, Cd, Cu and As from root to shoot in the course of the detoxifying process.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of Biological Sciences",
title = "Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill",
volume = "68",
number = "4",
pages = "829-835",
doi = "10.2298/ABS151011071V"
}

Vukojević, V., Trifković, J., Krgovic, R., Milojković-Opsenica, D., Marković, M., Amaizah, N. R. R.,& Mutić, J.. (2016). Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill. in Archives of Biological Sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 68(4), 829-835.
https://doi.org/10.2298/ABS151011071V

Vukojević V, Trifković J, Krgovic R, Milojković-Opsenica D, Marković M, Amaizah NRR, Mutić J. Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill. in Archives of Biological Sciences. 2016;68(4):829-835.
doi:10.2298/ABS151011071V .

Vukojević, Vesna, Trifković, Jelena, Krgovic, Rada, Milojković-Opsenica, Dušanka, Marković, Marijana, Amaizah, Naser Ramdan R., Mutić, Jelena, "Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill" in Archives of Biological Sciences, 68, no. 4 (2016):829-835,
https://doi.org/10.2298/ABS151011071V . .

TY  - JOUR
AU  - Jovanovic, Katarina K.
AU  - Tanic, Miljana
AU  - Ivanovic, Ivanka
AU  - Gligorijević, Nevenka
AU  - Dojčinović, Biljana
AU  - Radulovic, Sinisa
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4386
AB  - Ruthenium(II)-arene complexes are promising drug candidates for the therapy of solid tumors. In previous work, seven new compounds of the general formula [Ru(eta(6)-p-cymene)(L1-7)Cl] were synthesized and characterized, of which the complex with L = isoquinoline-3-carboxylic acid (RuT7) was two times as active on HeLa cells compared to normal cell line MRC-5, as indicated by ICso values determined after 48 h of incubation (45.4 +/- 3.0 vs. 842 +/- 5.7 mu M, respectively). In the present study, cell cycle analysis of HeLa cells treated with RuT7 showed S phase arrest and an increase in sub-G1 population. The apoptotic potential of the title compound was confirmed with the Annexin V-FITC/PI assay together with a morphological evaluation of cells using fluorescent microscopy. Analysis of the intracellular accumulation of ruthenium showed 8.9 ng Ru/10(6) cells after 6 h of incubation. To gain further insight in the molecular mechanism of action of RuT7 on HeLa cells, a whole-transcriptome microarray gene expression analysis was performed. Analysis of functional categories and signaling and biochemical pathways associated with the response of HeLa cells to treatment with RuT7 showed that it leads the cells through the intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage due to the action of reactive oxygen species, and through direct DNA binding of RuT7. Statistical analysis for enrichment of gene sets associated with known drug-induced toxicities identified fewer associated toxicity profiles in RuT7-treated cells compared to cisplatin treatment. Altogether these results provide the basis for further development of RuT7 in animal and pre-clinical studies as a potential drug candidate.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand
VL  - 163
SP  - 362
EP  - 373
DO  - 10.1016/j.jinorgbio.2016.04.011
ER  - 

@article{
author = "Jovanovic, Katarina K. and Tanic, Miljana and Ivanovic, Ivanka and Gligorijević, Nevenka and Dojčinović, Biljana and Radulovic, Sinisa",
year = "2016",
abstract = "Ruthenium(II)-arene complexes are promising drug candidates for the therapy of solid tumors. In previous work, seven new compounds of the general formula [Ru(eta(6)-p-cymene)(L1-7)Cl] were synthesized and characterized, of which the complex with L = isoquinoline-3-carboxylic acid (RuT7) was two times as active on HeLa cells compared to normal cell line MRC-5, as indicated by ICso values determined after 48 h of incubation (45.4 +/- 3.0 vs. 842 +/- 5.7 mu M, respectively). In the present study, cell cycle analysis of HeLa cells treated with RuT7 showed S phase arrest and an increase in sub-G1 population. The apoptotic potential of the title compound was confirmed with the Annexin V-FITC/PI assay together with a morphological evaluation of cells using fluorescent microscopy. Analysis of the intracellular accumulation of ruthenium showed 8.9 ng Ru/10(6) cells after 6 h of incubation. To gain further insight in the molecular mechanism of action of RuT7 on HeLa cells, a whole-transcriptome microarray gene expression analysis was performed. Analysis of functional categories and signaling and biochemical pathways associated with the response of HeLa cells to treatment with RuT7 showed that it leads the cells through the intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage due to the action of reactive oxygen species, and through direct DNA binding of RuT7. Statistical analysis for enrichment of gene sets associated with known drug-induced toxicities identified fewer associated toxicity profiles in RuT7-treated cells compared to cisplatin treatment. Altogether these results provide the basis for further development of RuT7 in animal and pre-clinical studies as a potential drug candidate.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand",
volume = "163",
pages = "362-373",
doi = "10.1016/j.jinorgbio.2016.04.011"
}

Jovanovic, K. K., Tanic, M., Ivanovic, I., Gligorijević, N., Dojčinović, B.,& Radulovic, S.. (2016). Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand. in Journal of Inorganic Biochemistry
Elsevier., 163, 362-373.
https://doi.org/10.1016/j.jinorgbio.2016.04.011

Jovanovic KK, Tanic M, Ivanovic I, Gligorijević N, Dojčinović B, Radulovic S. Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand. in Journal of Inorganic Biochemistry. 2016;163:362-373.
doi:10.1016/j.jinorgbio.2016.04.011 .

Jovanovic, Katarina K., Tanic, Miljana, Ivanovic, Ivanka, Gligorijević, Nevenka, Dojčinović, Biljana, Radulovic, Sinisa, "Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand" in Journal of Inorganic Biochemistry, 163 (2016):362-373,
https://doi.org/10.1016/j.jinorgbio.2016.04.011 . .

TY  - JOUR
AU  - Jovanovic, Katarina K.
AU  - Tanic, Miljana
AU  - Ivanovic, Ivanka
AU  - Gligorijević, Nevenka
AU  - Dojčinović, Biljana
AU  - Radulovic, Sinisa
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1984
AB  - Ruthenium(II)-arene complexes are promising drug candidates for the therapy of solid tumors. In previous work, seven new compounds of the general formula [Ru(eta(6)-p-cymene)(L1-7)Cl] were synthesized and characterized, of which the complex with L = isoquinoline-3-carboxylic acid (RuT7) was two times as active on HeLa cells compared to normal cell line MRC-5, as indicated by ICso values determined after 48 h of incubation (45.4 +/- 3.0 vs. 842 +/- 5.7 mu M, respectively). In the present study, cell cycle analysis of HeLa cells treated with RuT7 showed S phase arrest and an increase in sub-G1 population. The apoptotic potential of the title compound was confirmed with the Annexin V-FITC/PI assay together with a morphological evaluation of cells using fluorescent microscopy. Analysis of the intracellular accumulation of ruthenium showed 8.9 ng Ru/10(6) cells after 6 h of incubation. To gain further insight in the molecular mechanism of action of RuT7 on HeLa cells, a whole-transcriptome microarray gene expression analysis was performed. Analysis of functional categories and signaling and biochemical pathways associated with the response of HeLa cells to treatment with RuT7 showed that it leads the cells through the intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage due to the action of reactive oxygen species, and through direct DNA binding of RuT7. Statistical analysis for enrichment of gene sets associated with known drug-induced toxicities identified fewer associated toxicity profiles in RuT7-treated cells compared to cisplatin treatment. Altogether these results provide the basis for further development of RuT7 in animal and pre-clinical studies as a potential drug candidate.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand
VL  - 163
SP  - 362
EP  - 373
DO  - 10.1016/j.jinorgbio.2016.04.011
ER  - 

@article{
author = "Jovanovic, Katarina K. and Tanic, Miljana and Ivanovic, Ivanka and Gligorijević, Nevenka and Dojčinović, Biljana and Radulovic, Sinisa",
year = "2016",
abstract = "Ruthenium(II)-arene complexes are promising drug candidates for the therapy of solid tumors. In previous work, seven new compounds of the general formula [Ru(eta(6)-p-cymene)(L1-7)Cl] were synthesized and characterized, of which the complex with L = isoquinoline-3-carboxylic acid (RuT7) was two times as active on HeLa cells compared to normal cell line MRC-5, as indicated by ICso values determined after 48 h of incubation (45.4 +/- 3.0 vs. 842 +/- 5.7 mu M, respectively). In the present study, cell cycle analysis of HeLa cells treated with RuT7 showed S phase arrest and an increase in sub-G1 population. The apoptotic potential of the title compound was confirmed with the Annexin V-FITC/PI assay together with a morphological evaluation of cells using fluorescent microscopy. Analysis of the intracellular accumulation of ruthenium showed 8.9 ng Ru/10(6) cells after 6 h of incubation. To gain further insight in the molecular mechanism of action of RuT7 on HeLa cells, a whole-transcriptome microarray gene expression analysis was performed. Analysis of functional categories and signaling and biochemical pathways associated with the response of HeLa cells to treatment with RuT7 showed that it leads the cells through the intrinsic (mitochondrial) apoptotic pathway, via indirect DNA damage due to the action of reactive oxygen species, and through direct DNA binding of RuT7. Statistical analysis for enrichment of gene sets associated with known drug-induced toxicities identified fewer associated toxicity profiles in RuT7-treated cells compared to cisplatin treatment. Altogether these results provide the basis for further development of RuT7 in animal and pre-clinical studies as a potential drug candidate.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand",
volume = "163",
pages = "362-373",
doi = "10.1016/j.jinorgbio.2016.04.011"
}

Jovanovic, K. K., Tanic, M., Ivanovic, I., Gligorijević, N., Dojčinović, B.,& Radulovic, S.. (2016). Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 163, 362-373.
https://doi.org/10.1016/j.jinorgbio.2016.04.011

Jovanovic KK, Tanic M, Ivanovic I, Gligorijević N, Dojčinović B, Radulovic S. Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand. in Journal of Inorganic Biochemistry. 2016;163:362-373.
doi:10.1016/j.jinorgbio.2016.04.011 .

Jovanovic, Katarina K., Tanic, Miljana, Ivanovic, Ivanka, Gligorijević, Nevenka, Dojčinović, Biljana, Radulovic, Sinisa, "Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand" in Journal of Inorganic Biochemistry, 163 (2016):362-373,
https://doi.org/10.1016/j.jinorgbio.2016.04.011 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Bajusz, David
AU  - Racz, Anita
AU  - Šegan, Sandra
AU  - Héberger, Karoly
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3182
AB  - Needs for fast, yet reliable means of assessing the lipophilicities of diverse compounds resulted in the development of various in silico and chromatographic approaches that are faster, cheaper, and greener compared to the traditional shake-flask method. However, at present no accepted "standard" approach exists for their comparison and selection of the most appropriate one(s). This is of utmost importance when it comes to the development of new lipophilicity indices, or the assessment of the lipophilicity of newly synthesized compounds. In this study, 50 well-known, diverse compounds of significant pharmaceutical and environmental importance have been selected and examined. Octanol-water partition coefficients have been measured with the shake-flask method for most of them. Their retentions have been studied in typical reversed thin-layer chromatographic systems, involving the most frequently employed stationary phases (octadecyl- and cyano-modified silica), and acetonitrile and methanol as mobile phase constituents. Twelve computationally estimated logP-s and twenty chromatographic indices together with the shake-flask octanol-water partition coefficient have been investigated with classical chemometric approaches such as principal component analysis (PCA), hierarchical cluster analysis (HCA), Pearson's and Spearman's correlation matrices, as well as novel non-parametric methods: sum of ranking differences (SRD) and generalized pairwise correlation method (GPCM). Novel SRD and GPCM methods have been introduced based on the Comparisons with One VAriable (lipophilicity metric) at a Time (COVAT). For the visualization of COVAT results, a heatmap format was introduced. Analysis of variance (ANOVA) was applied to reveal the dominant factors between computational logPs and various chromatographic measures. In consensus-based comparisons, the shake-flask method performed the best, closely followed by computational estimates, while the chromatographic estimates often overlap with in silico assessments, mostly with methods involving octadecyl-modified silica stationary phases. The ones that employ cyano-modified silica perform generally worse. The introduction of alternative coloring schemes for the covariance matrices and SRD/GPCM heatmaps enables the discovery of intrinsic relationships among lipophilicity scales and the selection of best/worst measures. Closest to the recommended logK(ow) values are ClogP and the first principal component scores obtained on octadecyl-silica stationary phase in combination with methanol-water mobile phase, while the usage of slopes derived from Soczewinski-Matyisik equation should be avoided. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices
VL  - 127
SP  - 81
EP  - 93
DO  - 10.1016/j.jpba.2016.04.001
ER  - 

@article{
author = "Andrić, Filip and Bajusz, David and Racz, Anita and Šegan, Sandra and Héberger, Karoly",
year = "2016",
abstract = "Needs for fast, yet reliable means of assessing the lipophilicities of diverse compounds resulted in the development of various in silico and chromatographic approaches that are faster, cheaper, and greener compared to the traditional shake-flask method. However, at present no accepted "standard" approach exists for their comparison and selection of the most appropriate one(s). This is of utmost importance when it comes to the development of new lipophilicity indices, or the assessment of the lipophilicity of newly synthesized compounds. In this study, 50 well-known, diverse compounds of significant pharmaceutical and environmental importance have been selected and examined. Octanol-water partition coefficients have been measured with the shake-flask method for most of them. Their retentions have been studied in typical reversed thin-layer chromatographic systems, involving the most frequently employed stationary phases (octadecyl- and cyano-modified silica), and acetonitrile and methanol as mobile phase constituents. Twelve computationally estimated logP-s and twenty chromatographic indices together with the shake-flask octanol-water partition coefficient have been investigated with classical chemometric approaches such as principal component analysis (PCA), hierarchical cluster analysis (HCA), Pearson's and Spearman's correlation matrices, as well as novel non-parametric methods: sum of ranking differences (SRD) and generalized pairwise correlation method (GPCM). Novel SRD and GPCM methods have been introduced based on the Comparisons with One VAriable (lipophilicity metric) at a Time (COVAT). For the visualization of COVAT results, a heatmap format was introduced. Analysis of variance (ANOVA) was applied to reveal the dominant factors between computational logPs and various chromatographic measures. In consensus-based comparisons, the shake-flask method performed the best, closely followed by computational estimates, while the chromatographic estimates often overlap with in silico assessments, mostly with methods involving octadecyl-modified silica stationary phases. The ones that employ cyano-modified silica perform generally worse. The introduction of alternative coloring schemes for the covariance matrices and SRD/GPCM heatmaps enables the discovery of intrinsic relationships among lipophilicity scales and the selection of best/worst measures. Closest to the recommended logK(ow) values are ClogP and the first principal component scores obtained on octadecyl-silica stationary phase in combination with methanol-water mobile phase, while the usage of slopes derived from Soczewinski-Matyisik equation should be avoided. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices",
volume = "127",
pages = "81-93",
doi = "10.1016/j.jpba.2016.04.001"
}

Andrić, F., Bajusz, D., Racz, A., Šegan, S.,& Héberger, K.. (2016). Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 127, 81-93.
https://doi.org/10.1016/j.jpba.2016.04.001

Andrić F, Bajusz D, Racz A, Šegan S, Héberger K. Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices. in Journal of Pharmaceutical and Biomedical Analysis. 2016;127:81-93.
doi:10.1016/j.jpba.2016.04.001 .

Andrić, Filip, Bajusz, David, Racz, Anita, Šegan, Sandra, Héberger, Karoly, "Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices" in Journal of Pharmaceutical and Biomedical Analysis, 127 (2016):81-93,
https://doi.org/10.1016/j.jpba.2016.04.001 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Dramićanin, Aleksandra M.
AU  - Majstorović, Helena
AU  - Milojković-Opsenica, Dušanka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3156
AB  - Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - Journal of Chromatography A
T1  - Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography
VL  - 1458
SP  - 136
EP  - 144
DO  - 10.1016/j.chroma.2016.06.063
ER  - 

@article{
author = "Andrić, Filip and Šegan, Sandra and Dramićanin, Aleksandra M. and Majstorović, Helena and Milojković-Opsenica, Dušanka",
year = "2016",
abstract = "Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "Journal of Chromatography A",
title = "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography",
volume = "1458",
pages = "136-144",
doi = "10.1016/j.chroma.2016.06.063"
}

Andrić, F., Šegan, S., Dramićanin, A. M., Majstorović, H.,& Milojković-Opsenica, D.. (2016). Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A
Elsevier., 1458, 136-144.
https://doi.org/10.1016/j.chroma.2016.06.063

Andrić F, Šegan S, Dramićanin AM, Majstorović H, Milojković-Opsenica D. Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A. 2016;1458:136-144.
doi:10.1016/j.chroma.2016.06.063 .

Andrić, Filip, Šegan, Sandra, Dramićanin, Aleksandra M., Majstorović, Helena, Milojković-Opsenica, Dušanka, "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography" in Journal of Chromatography A, 1458 (2016):136-144,
https://doi.org/10.1016/j.chroma.2016.06.063 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Dramićanin, Aleksandra
AU  - Majstorovic, Helena
AU  - Milojković-Opsenica, Dušanka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1849
AB  - Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems.
PB  - Elsevier
T2  - Journal of Chromatography A
T1  - Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography
VL  - 1458
SP  - 136
EP  - 144
DO  - 10.1016/j.chroma.2016.06.063
ER  - 

@article{
author = "Andrić, Filip and Šegan, Sandra and Dramićanin, Aleksandra and Majstorovic, Helena and Milojković-Opsenica, Dušanka",
year = "2016",
abstract = "Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems.",
publisher = "Elsevier",
journal = "Journal of Chromatography A",
title = "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography",
volume = "1458",
pages = "136-144",
doi = "10.1016/j.chroma.2016.06.063"
}

Andrić, F., Šegan, S., Dramićanin, A., Majstorovic, H.,& Milojković-Opsenica, D.. (2016). Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A
Elsevier., 1458, 136-144.
https://doi.org/10.1016/j.chroma.2016.06.063

Andrić F, Šegan S, Dramićanin A, Majstorovic H, Milojković-Opsenica D. Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A. 2016;1458:136-144.
doi:10.1016/j.chroma.2016.06.063 .

Andrić, Filip, Šegan, Sandra, Dramićanin, Aleksandra, Majstorovic, Helena, Milojković-Opsenica, Dušanka, "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography" in Journal of Chromatography A, 1458 (2016):136-144,
https://doi.org/10.1016/j.chroma.2016.06.063 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Bajusz, David
AU  - Racz, Anita
AU  - Šegan, Sandra
AU  - Heberger, Karoly
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1848
AB  - Needs for fast, yet reliable means of assessing the lipophilicities of diverse compounds resulted in the development of various in silico and chromatographic approaches that are faster, cheaper, and greener compared to the traditional shake-flask method. However, at present no accepted "standard" approach exists for their comparison and selection of the most appropriate one(s). This is of utmost importance when it comes to the development of new lipophilicity indices, or the assessment of the lipophilicity of newly synthesized compounds. In this study, 50 well-known, diverse compounds of significant pharmaceutical and environmental importance have been selected and examined. Octanol-water partition coefficients have been measured with the shake-flask method for most of them. Their retentions have been studied in typical reversed thin-layer chromatographic systems, involving the most frequently employed stationary phases (octadecyl- and cyano-modified silica), and acetonitrile and methanol as mobile phase constituents. Twelve computationally estimated logP-s and twenty chromatographic indices together with the shake-flask octanol-water partition coefficient have been investigated with classical chemometric approaches such as principal component analysis (PCA), hierarchical cluster analysis (HCA), Pearson's and Spearman's correlation matrices, as well as novel non-parametric methods: sum of ranking differences (SRD) and generalized pairwise correlation method (GPCM). Novel SRD and GPCM methods have been introduced based on the Comparisons with One VAriable (lipophilicity metric) at a Time (COVAT). For the visualization of COVAT results, a heatmap format was introduced. Analysis of variance (ANOVA) was applied to reveal the dominant factors between computational logPs and various chromatographic measures. In consensus-based comparisons, the shake-flask method performed the best, closely followed by computational estimates, while the chromatographic estimates often overlap with in silico assessments, mostly with methods involving octadecyl-modified silica stationary phases. The ones that employ cyano-modified silica perform generally worse. The introduction of alternative coloring schemes for the covariance matrices and SRD/GPCM heatmaps enables the discovery of intrinsic relationships among lipophilicity scales and the selection of best/worst measures. Closest to the recommended logK(ow) values are ClogP and the first principal component scores obtained on octadecyl-silica stationary phase in combination with methanol-water mobile phase, while the usage of slopes derived from Soczewinski-Matyisik equation should be avoided.
PB  - Elsevier
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices
VL  - 127
SP  - 81
EP  - 93
DO  - 10.1016/j.jpba.2016.04.001
ER  - 

@article{
author = "Andrić, Filip and Bajusz, David and Racz, Anita and Šegan, Sandra and Heberger, Karoly",
year = "2016",
abstract = "Needs for fast, yet reliable means of assessing the lipophilicities of diverse compounds resulted in the development of various in silico and chromatographic approaches that are faster, cheaper, and greener compared to the traditional shake-flask method. However, at present no accepted "standard" approach exists for their comparison and selection of the most appropriate one(s). This is of utmost importance when it comes to the development of new lipophilicity indices, or the assessment of the lipophilicity of newly synthesized compounds. In this study, 50 well-known, diverse compounds of significant pharmaceutical and environmental importance have been selected and examined. Octanol-water partition coefficients have been measured with the shake-flask method for most of them. Their retentions have been studied in typical reversed thin-layer chromatographic systems, involving the most frequently employed stationary phases (octadecyl- and cyano-modified silica), and acetonitrile and methanol as mobile phase constituents. Twelve computationally estimated logP-s and twenty chromatographic indices together with the shake-flask octanol-water partition coefficient have been investigated with classical chemometric approaches such as principal component analysis (PCA), hierarchical cluster analysis (HCA), Pearson's and Spearman's correlation matrices, as well as novel non-parametric methods: sum of ranking differences (SRD) and generalized pairwise correlation method (GPCM). Novel SRD and GPCM methods have been introduced based on the Comparisons with One VAriable (lipophilicity metric) at a Time (COVAT). For the visualization of COVAT results, a heatmap format was introduced. Analysis of variance (ANOVA) was applied to reveal the dominant factors between computational logPs and various chromatographic measures. In consensus-based comparisons, the shake-flask method performed the best, closely followed by computational estimates, while the chromatographic estimates often overlap with in silico assessments, mostly with methods involving octadecyl-modified silica stationary phases. The ones that employ cyano-modified silica perform generally worse. The introduction of alternative coloring schemes for the covariance matrices and SRD/GPCM heatmaps enables the discovery of intrinsic relationships among lipophilicity scales and the selection of best/worst measures. Closest to the recommended logK(ow) values are ClogP and the first principal component scores obtained on octadecyl-silica stationary phase in combination with methanol-water mobile phase, while the usage of slopes derived from Soczewinski-Matyisik equation should be avoided.",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices",
volume = "127",
pages = "81-93",
doi = "10.1016/j.jpba.2016.04.001"
}

Andrić, F., Bajusz, D., Racz, A., Šegan, S.,& Heberger, K.. (2016). Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 127, 81-93.
https://doi.org/10.1016/j.jpba.2016.04.001

Andrić F, Bajusz D, Racz A, Šegan S, Heberger K. Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices. in Journal of Pharmaceutical and Biomedical Analysis. 2016;127:81-93.
doi:10.1016/j.jpba.2016.04.001 .

Andrić, Filip, Bajusz, David, Racz, Anita, Šegan, Sandra, Heberger, Karoly, "Multivariate assessment of lipophilicity scales-computational and reversed phase thin-layer chromatographic indices" in Journal of Pharmaceutical and Biomedical Analysis, 127 (2016):81-93,
https://doi.org/10.1016/j.jpba.2016.04.001 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Tešić, Živoslav
AU  - Milojković-Opsenica, Dušanka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1861
AB  - The soil-water partition coefficient normalized to the soil organic carbon content (K-OC) is one of essential properties governing the fate of organic chemicals in the soil-water compartment. It is a parameter of utmost importance when it comes to the removal of toxic organics in the waste water management facilities or placing a new chemical on the market. Since direct determination of K-OC based on tests involving soil-sorption measurements are expensive, time-consuming, tedious, and subject to different experimental difficulties and artifacts, different alternative methods have been developed for rapid indirect experimental determination and estimation of K-OC values. In the scope of the present work, we provide critical and historical overview of development and application of the main chromatographic methods, both high-performance liquid chromatography and thin-layer chromatographic techniques for indirect experimental determination of the soil-water partition coefficient as well as mobility assessment of environmentally important compounds in the soil. Chromatographic methods proved to be promising in this field, which is clearly demonstrated by their implementation in official guidelines for testing the chemicals.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Chromatographic methods in determination of the soil-water partition coefficient
VL  - 39
IS  - 5-6
SP  - 249
EP  - 256
DO  - 10.1080/10826076.2016.1163173
ER  - 

@article{
author = "Andrić, Filip and Šegan, Sandra and Tešić, Živoslav and Milojković-Opsenica, Dušanka",
year = "2016",
abstract = "The soil-water partition coefficient normalized to the soil organic carbon content (K-OC) is one of essential properties governing the fate of organic chemicals in the soil-water compartment. It is a parameter of utmost importance when it comes to the removal of toxic organics in the waste water management facilities or placing a new chemical on the market. Since direct determination of K-OC based on tests involving soil-sorption measurements are expensive, time-consuming, tedious, and subject to different experimental difficulties and artifacts, different alternative methods have been developed for rapid indirect experimental determination and estimation of K-OC values. In the scope of the present work, we provide critical and historical overview of development and application of the main chromatographic methods, both high-performance liquid chromatography and thin-layer chromatographic techniques for indirect experimental determination of the soil-water partition coefficient as well as mobility assessment of environmentally important compounds in the soil. Chromatographic methods proved to be promising in this field, which is clearly demonstrated by their implementation in official guidelines for testing the chemicals.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Chromatographic methods in determination of the soil-water partition coefficient",
volume = "39",
number = "5-6",
pages = "249-256",
doi = "10.1080/10826076.2016.1163173"
}

Andrić, F., Šegan, S., Tešić, Ž.,& Milojković-Opsenica, D.. (2016). Chromatographic methods in determination of the soil-water partition coefficient. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 39(5-6), 249-256.
https://doi.org/10.1080/10826076.2016.1163173

Andrić F, Šegan S, Tešić Ž, Milojković-Opsenica D. Chromatographic methods in determination of the soil-water partition coefficient. in Journal of Liquid Chromatography & Related Technologies. 2016;39(5-6):249-256.
doi:10.1080/10826076.2016.1163173 .

Andrić, Filip, Šegan, Sandra, Tešić, Živoslav, Milojković-Opsenica, Dušanka, "Chromatographic methods in determination of the soil-water partition coefficient" in Journal of Liquid Chromatography & Related Technologies, 39, no. 5-6 (2016):249-256,
https://doi.org/10.1080/10826076.2016.1163173 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Pešić, Mirjana B.
AU  - Mosic, Mirjana D
AU  - Dojčinović, Biljana
AU  - Natić, Maja
AU  - Trifković, Jelena
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1678
AB  - In this study we analysed mineral composition of bee pollen of different plant origin collected across Serbia using inductively coupled plasma - optical emission spectrometry. The most abundant elements were potassium, calcium, and magnesium. The samples were also exceptionally rich in iron and zinc, which are very important as nutrients. Judging by our findings, mineral composition of bee pollen much more depends on the type of pollen-producing plant than on its geographical origin.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
T1  - Mineral content of bee pollen from Serbia
VL  - 66
IS  - 4
SP  - 251
EP  - 258
DO  - 10.1515/aiht-2015-66-2630
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Pešić, Mirjana B. and Mosic, Mirjana D and Dojčinović, Biljana and Natić, Maja and Trifković, Jelena",
year = "2015",
abstract = "In this study we analysed mineral composition of bee pollen of different plant origin collected across Serbia using inductively coupled plasma - optical emission spectrometry. The most abundant elements were potassium, calcium, and magnesium. The samples were also exceptionally rich in iron and zinc, which are very important as nutrients. Judging by our findings, mineral composition of bee pollen much more depends on the type of pollen-producing plant than on its geographical origin.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology",
title = "Mineral content of bee pollen from Serbia",
volume = "66",
number = "4",
pages = "251-258",
doi = "10.1515/aiht-2015-66-2630"
}

Kostić, A. Ž., Pešić, M. B., Mosic, M. D., Dojčinović, B., Natić, M.,& Trifković, J.. (2015). Mineral content of bee pollen from Serbia. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 66(4), 251-258.
https://doi.org/10.1515/aiht-2015-66-2630

Kostić AŽ, Pešić MB, Mosic MD, Dojčinović B, Natić M, Trifković J. Mineral content of bee pollen from Serbia. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology. 2015;66(4):251-258.
doi:10.1515/aiht-2015-66-2630 .

Kostić, Aleksandar Ž., Pešić, Mirjana B., Mosic, Mirjana D, Dojčinović, Biljana, Natić, Maja, Trifković, Jelena, "Mineral content of bee pollen from Serbia" in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology, 66, no. 4 (2015):251-258,
https://doi.org/10.1515/aiht-2015-66-2630 . .