TY  - JOUR
AU  - Nisavic, Marija
AU  - Janjić, Goran
AU  - Hozic, Amela
AU  - Petkovic, Marijana
AU  - Milčić, Miloš
AU  - Vujčić, Zoran
AU  - Cindric, Mario
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2296
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
VL  - 10
IS  - 4
SP  - 587
EP  - 594
DO  - 10.1039/c7mt00330g
ER  - 

@article{
author = "Nisavic, Marija and Janjić, Goran and Hozic, Amela and Petkovic, Marijana and Milčić, Miloš and Vujčić, Zoran and Cindric, Mario",
year = "2018",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
volume = "10",
number = "4",
pages = "587-594",
doi = "10.1039/c7mt00330g"
}

Nisavic, M., Janjić, G., Hozic, A., Petkovic, M., Milčić, M., Vujčić, Z.,& Cindric, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics
Royal Soc Chemistry, Cambridge., 10(4), 587-594.
https://doi.org/10.1039/c7mt00330g

Nisavic M, Janjić G, Hozic A, Petkovic M, Milčić M, Vujčić Z, Cindric M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/c7mt00330g .

Nisavic, Marija, Janjić, Goran, Hozic, Amela, Petkovic, Marijana, Milčić, Miloš, Vujčić, Zoran, Cindric, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/c7mt00330g . .

TY  - JOUR
AU  - Nisavic, Marija
AU  - Janjić, Goran
AU  - Hozic, Amela
AU  - Petkovic, Marijana
AU  - Milčić, Miloš
AU  - Vujčić, Zoran
AU  - Cindric, Mario
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3339
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
VL  - 10
IS  - 4
SP  - 587
EP  - 594
DO  - 10.1039/c7mt00330g
ER  - 

@article{
author = "Nisavic, Marija and Janjić, Goran and Hozic, Amela and Petkovic, Marijana and Milčić, Miloš and Vujčić, Zoran and Cindric, Mario",
year = "2018",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
volume = "10",
number = "4",
pages = "587-594",
doi = "10.1039/c7mt00330g"
}

Nisavic, M., Janjić, G., Hozic, A., Petkovic, M., Milčić, M., Vujčić, Z.,& Cindric, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics
Royal Soc Chemistry, Cambridge., 10(4), 587-594.
https://doi.org/10.1039/c7mt00330g

Nisavic M, Janjić G, Hozic A, Petkovic M, Milčić M, Vujčić Z, Cindric M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/c7mt00330g .

Nisavic, Marija, Janjić, Goran, Hozic, Amela, Petkovic, Marijana, Milčić, Miloš, Vujčić, Zoran, Cindric, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/c7mt00330g . .

TY  - JOUR
AU  - Dordevic, Milena
AU  - Jeremić, Dejan
AU  - Kaluđerović, Goran N.
AU  - Gómez-Ruiz, Santiago
AU  - Anđelković, Boban D.
AU  - Radanović, Dušanka
AU  - Brčeski, Ilija
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1429
AB  - Three new 1D coordination polymers, [Pb-2(CH3SO3)(4)(H2O)(2)](n), [Hg(CH3SO3)(2)(H2O)(2)](n) and [Sr(CH3SO3)(2)(H2O)](n), were synthesized as large single crystals. The crystals were analyzed and characterized by the means of X-ray analysis, IR and NMR spectroscopy, elemental analysis and solid state UV-Vis spectroscopy. The formation of 1D polymeric chains in the crystal structures of the title compounds is affected by the various bonding modes of the bridging methanesulfonate groups. The studied compounds showed no decomposition in the air.
PB  - Oxford : Pergamon-Elsevier Science Ltd
T2  - Polyhedron
T1  - Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers
VL  - 80
SP  - 282
EP  - 289
DO  - 10.1016/j.poly.2014.05.056
ER  - 

@article{
author = "Dordevic, Milena and Jeremić, Dejan and Kaluđerović, Goran N. and Gómez-Ruiz, Santiago and Anđelković, Boban D. and Radanović, Dušanka and Brčeski, Ilija",
year = "2014",
abstract = "Three new 1D coordination polymers, [Pb-2(CH3SO3)(4)(H2O)(2)](n), [Hg(CH3SO3)(2)(H2O)(2)](n) and [Sr(CH3SO3)(2)(H2O)](n), were synthesized as large single crystals. The crystals were analyzed and characterized by the means of X-ray analysis, IR and NMR spectroscopy, elemental analysis and solid state UV-Vis spectroscopy. The formation of 1D polymeric chains in the crystal structures of the title compounds is affected by the various bonding modes of the bridging methanesulfonate groups. The studied compounds showed no decomposition in the air.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "Polyhedron",
title = "Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers",
volume = "80",
pages = "282-289",
doi = "10.1016/j.poly.2014.05.056"
}

Dordevic, M., Jeremić, D., Kaluđerović, G. N., Gómez-Ruiz, S., Anđelković, B. D., Radanović, D.,& Brčeski, I.. (2014). Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers. in Polyhedron
Oxford : Pergamon-Elsevier Science Ltd., 80, 282-289.
https://doi.org/10.1016/j.poly.2014.05.056

Dordevic M, Jeremić D, Kaluđerović GN, Gómez-Ruiz S, Anđelković BD, Radanović D, Brčeski I. Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers. in Polyhedron. 2014;80:282-289.
doi:10.1016/j.poly.2014.05.056 .

Dordevic, Milena, Jeremić, Dejan, Kaluđerović, Goran N., Gómez-Ruiz, Santiago, Anđelković, Boban D., Radanović, Dušanka, Brčeski, Ilija, "Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers" in Polyhedron, 80 (2014):282-289,
https://doi.org/10.1016/j.poly.2014.05.056 . .

TY  - JOUR
AU  - Dordevic, Milena
AU  - Jeremić, Dejan
AU  - Kaluđerović, Goran N.
AU  - Gómez-Ruiz, Santiago
AU  - Anđelković, Boban D.
AU  - Radanović, Dušanka
AU  - Brčeski, Ilija
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3164
AB  - Three new 1D coordination polymers, [Pb-2(CH3SO3)(4)(H2O)(2)](n), [Hg(CH3SO3)(2)(H2O)(2)](n) and [Sr(CH3SO3)(2)(H2O)](n), were synthesized as large single crystals. The crystals were analyzed and characterized by the means of X-ray analysis, IR and NMR spectroscopy, elemental analysis and solid state UV-Vis spectroscopy. The formation of 1D polymeric chains in the crystal structures of the title compounds is affected by the various bonding modes of the bridging methanesulfonate groups. The studied compounds showed no decomposition in the air.
PB  - Oxford : Pergamon-Elsevier Science Ltd
T2  - Polyhedron
T1  - Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers
VL  - 80
SP  - 282
EP  - 289
DO  - 10.1016/j.poly.2014.05.056
ER  - 

@article{
author = "Dordevic, Milena and Jeremić, Dejan and Kaluđerović, Goran N. and Gómez-Ruiz, Santiago and Anđelković, Boban D. and Radanović, Dušanka and Brčeski, Ilija",
year = "2014",
abstract = "Three new 1D coordination polymers, [Pb-2(CH3SO3)(4)(H2O)(2)](n), [Hg(CH3SO3)(2)(H2O)(2)](n) and [Sr(CH3SO3)(2)(H2O)](n), were synthesized as large single crystals. The crystals were analyzed and characterized by the means of X-ray analysis, IR and NMR spectroscopy, elemental analysis and solid state UV-Vis spectroscopy. The formation of 1D polymeric chains in the crystal structures of the title compounds is affected by the various bonding modes of the bridging methanesulfonate groups. The studied compounds showed no decomposition in the air.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "Polyhedron",
title = "Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers",
volume = "80",
pages = "282-289",
doi = "10.1016/j.poly.2014.05.056"
}

Dordevic, M., Jeremić, D., Kaluđerović, G. N., Gómez-Ruiz, S., Anđelković, B. D., Radanović, D.,& Brčeski, I.. (2014). Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers. in Polyhedron
Oxford : Pergamon-Elsevier Science Ltd., 80, 282-289.
https://doi.org/10.1016/j.poly.2014.05.056

Dordevic M, Jeremić D, Kaluđerović GN, Gómez-Ruiz S, Anđelković BD, Radanović D, Brčeski I. Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers. in Polyhedron. 2014;80:282-289.
doi:10.1016/j.poly.2014.05.056 .

Dordevic, Milena, Jeremić, Dejan, Kaluđerović, Goran N., Gómez-Ruiz, Santiago, Anđelković, Boban D., Radanović, Dušanka, Brčeski, Ilija, "Synthesis and spectroscopic properties of large single-crystals of Pb(II), Hg(II) and Sr(II) methanesulfonato 1D coordination polymers" in Polyhedron, 80 (2014):282-289,
https://doi.org/10.1016/j.poly.2014.05.056 . .