TY  - JOUR
AU  - Šunderić, Miloš
AU  - Gligorijević, Nikola
AU  - Milčić, Miloš
AU  - Minić, Simeon
AU  - Nedić, Olgica
AU  - Nikolić, Milan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6471
AB  - Under simulated physiological conditions, this study investigates the interaction between nutraceutical phycocyanobilin (PCB) and the universal anti-protease protein human alpha-2-macroglobulin (a2M). Extensive molecular docking analyses on multiple a2M conformations, spectroscopic techniques, and a2M activity assays were utilized to examine the complex formation. The results revealed that for every protein conformation, two high energy binding sites exist: the first, conformationally independent, at the interface region between two monomer chains and the second, conformationally dependent, in the pocket composed of amino acids from four distinct domains (TED, RBD, CUB, and MG2) of the single protein chain. Spectrofluorimetric measurements indicated a moderate affinity between a2M and PCB with a moderately high binding constant of 6.3 x 10^5 M^-1 at 25 °C. The binding of PCB to a2M resulted in minor changes in the secondary structure content of a2M. Furthermore, PCB protected a2M from oxidation and preserved its anti-protease activity in the oxidative environment. These findings suggest that PCB binding could indirectly impact the body’s response to oxidative stress by influencing a2M’s role in controlling enzyme activity during the inflammatory process.
PB  - Taylor & Francis Group
T2  - Journal of Biomolecular Structure and Dynamics
T1  - Phycocyanobilin is a new binding partner of human alpha-2-macroglobulin that protects the protein against oxidative stress
DO  - 10.1080/07391102.2023.2248273
ER  - 

@article{
author = "Šunderić, Miloš and Gligorijević, Nikola and Milčić, Miloš and Minić, Simeon and Nedić, Olgica and Nikolić, Milan",
year = "2023",
abstract = "Under simulated physiological conditions, this study investigates the interaction between nutraceutical phycocyanobilin (PCB) and the universal anti-protease protein human alpha-2-macroglobulin (a2M). Extensive molecular docking analyses on multiple a2M conformations, spectroscopic techniques, and a2M activity assays were utilized to examine the complex formation. The results revealed that for every protein conformation, two high energy binding sites exist: the first, conformationally independent, at the interface region between two monomer chains and the second, conformationally dependent, in the pocket composed of amino acids from four distinct domains (TED, RBD, CUB, and MG2) of the single protein chain. Spectrofluorimetric measurements indicated a moderate affinity between a2M and PCB with a moderately high binding constant of 6.3 x 10^5 M^-1 at 25 °C. The binding of PCB to a2M resulted in minor changes in the secondary structure content of a2M. Furthermore, PCB protected a2M from oxidation and preserved its anti-protease activity in the oxidative environment. These findings suggest that PCB binding could indirectly impact the body’s response to oxidative stress by influencing a2M’s role in controlling enzyme activity during the inflammatory process.",
publisher = "Taylor & Francis Group",
journal = "Journal of Biomolecular Structure and Dynamics",
title = "Phycocyanobilin is a new binding partner of human alpha-2-macroglobulin that protects the protein against oxidative stress",
doi = "10.1080/07391102.2023.2248273"
}

Šunderić, M., Gligorijević, N., Milčić, M., Minić, S., Nedić, O.,& Nikolić, M.. (2023). Phycocyanobilin is a new binding partner of human alpha-2-macroglobulin that protects the protein against oxidative stress. in Journal of Biomolecular Structure and Dynamics
Taylor & Francis Group..
https://doi.org/10.1080/07391102.2023.2248273

Šunderić M, Gligorijević N, Milčić M, Minić S, Nedić O, Nikolić M. Phycocyanobilin is a new binding partner of human alpha-2-macroglobulin that protects the protein against oxidative stress. in Journal of Biomolecular Structure and Dynamics. 2023;.
doi:10.1080/07391102.2023.2248273 .

Šunderić, Miloš, Gligorijević, Nikola, Milčić, Miloš, Minić, Simeon, Nedić, Olgica, Nikolić, Milan, "Phycocyanobilin is a new binding partner of human alpha-2-macroglobulin that protects the protein against oxidative stress" in Journal of Biomolecular Structure and Dynamics (2023),
https://doi.org/10.1080/07391102.2023.2248273 . .

TY  - JOUR
AU  - Nedić, Olgica
AU  - Penezić, Ana
AU  - Minić, Simeon
AU  - Radomirović, Mirjana
AU  - Nikolić, Milan
AU  - Ćirković Veličković, Tanja
AU  - Gligorijević, Nikola
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6485
AB  - Common to all biological systems and living organisms are molecular interactions, which
may lead to specific physiological events. Most often, a cascade of events occurs, establishing an
equilibrium between possibly competing and/or synergistic processes. Biochemical pathways that
sustain life depend on multiple intrinsic and extrinsic factors contributing to aging and/or diseases.
This article deals with food antioxidants and human proteins from the circulation, their interaction,
their effect on the structure, properties, and function of antioxidant-bound proteins, and the possible
impact of complex formation on antioxidants. An overview of studies examining interactions between
individual antioxidant compounds and major blood proteins is presented with findings. Investigating
antioxidant/protein interactions at the level of the human organism and determining antioxidant
distribution between proteins and involvement in the particular physiological role is a very complex
and challenging task. However, by knowing the role of a particular protein in certain pathology or
aging, and the effect exerted by a particular antioxidant bound to it, it is possible to recommend
specific food intake or resistance to it to improve the condition or slow down the process.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Antioxidants
T1  - Food Antioxidants and Their Interaction with Human Proteins
VL  - 12
IS  - 4
SP  - 815
DO  - 10.3390/antiox12040815
ER  - 

@article{
author = "Nedić, Olgica and Penezić, Ana and Minić, Simeon and Radomirović, Mirjana and Nikolić, Milan and Ćirković Veličković, Tanja and Gligorijević, Nikola",
year = "2023",
abstract = "Common to all biological systems and living organisms are molecular interactions, which
may lead to specific physiological events. Most often, a cascade of events occurs, establishing an
equilibrium between possibly competing and/or synergistic processes. Biochemical pathways that
sustain life depend on multiple intrinsic and extrinsic factors contributing to aging and/or diseases.
This article deals with food antioxidants and human proteins from the circulation, their interaction,
their effect on the structure, properties, and function of antioxidant-bound proteins, and the possible
impact of complex formation on antioxidants. An overview of studies examining interactions between
individual antioxidant compounds and major blood proteins is presented with findings. Investigating
antioxidant/protein interactions at the level of the human organism and determining antioxidant
distribution between proteins and involvement in the particular physiological role is a very complex
and challenging task. However, by knowing the role of a particular protein in certain pathology or
aging, and the effect exerted by a particular antioxidant bound to it, it is possible to recommend
specific food intake or resistance to it to improve the condition or slow down the process.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Antioxidants",
title = "Food Antioxidants and Their Interaction with Human Proteins",
volume = "12",
number = "4",
pages = "815",
doi = "10.3390/antiox12040815"
}

Nedić, O., Penezić, A., Minić, S., Radomirović, M., Nikolić, M., Ćirković Veličković, T.,& Gligorijević, N.. (2023). Food Antioxidants and Their Interaction with Human Proteins. in Antioxidants
Multidisciplinary Digital Publishing Institute (MDPI)., 12(4), 815.
https://doi.org/10.3390/antiox12040815

Nedić O, Penezić A, Minić S, Radomirović M, Nikolić M, Ćirković Veličković T, Gligorijević N. Food Antioxidants and Their Interaction with Human Proteins. in Antioxidants. 2023;12(4):815.
doi:10.3390/antiox12040815 .

Nedić, Olgica, Penezić, Ana, Minić, Simeon, Radomirović, Mirjana, Nikolić, Milan, Ćirković Veličković, Tanja, Gligorijević, Nikola, "Food Antioxidants and Their Interaction with Human Proteins" in Antioxidants, 12, no. 4 (2023):815,
https://doi.org/10.3390/antiox12040815 . .

TY  - CONF
AU  - Četić, Danilo
AU  - Miljuš, Goran
AU  - Dobrijević, Zorana
AU  - Gligorijević, Nikola
AU  - Vilotić, Aleksandra
AU  - Nedić, Olgica
AU  - Penezić, Ana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6663
AB  - Human transferrin (Tf) is a bilobal 76 kDa iron-binding glycoprotein present in human
serum. Each lobe has the ability to bind one ferric ion (Fe3+) and a single synergistic
bicarbonate anion. The main role of Tf is to transport Fe3+ ions through the circulation to
cells, via interaction with transferrin receptor (TFR) on the cell surface. Previously
described methods for Tf isolation and purification are either very time-consuming or
provide Tf of lower final purity. Here we describe a fast and simple FPLC method for the
isolation and purification of Tf from human serum. Serum samples were prepared by
precipitation, while protein purification was performed on FPLC system, using an anionexchange
column. Several different buffers at the same pH were tested. Tf purified by this
method was analyzed by Western blot, followed by immunodetection, as well as with
silver staining after SDS PAGE. Its functionality was tested with respect to iron-binding
capacity (ferozzine method) and its ability to interact with TFR by immunofluorescent
staining. The conformation of purified Tf was analyzed by recording intrinsic fluorescent
emmision spectra originating from Trp residues. The method itself is highly reproducible
(intra- and interday), easy to perform (only two steps) and fast (under an hour), yielding
98% to 99% pure Tf with all buffers. Purified Tf was shown to have retained its ironbinding
capacity, as well as the ability to interact with TFR. Purified Tf also retained its
native three-dimensional structure. Described method for the isolation and purification of
Tf is fast, simple and highly reproducible, yielding a functional Tf of high purity in its
native state while offering the flexibility of using different buffer systems. All of these
features make this protocol a method of choice for the isolation and purification of Tf on a
semi-preparative scale.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
T1  - Simple two-step semi-preparative isolation and purification of transferrin from human serum
SP  - 66
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_cer_6663
ER  - 

@conference{
author = "Četić, Danilo and Miljuš, Goran and Dobrijević, Zorana and Gligorijević, Nikola and Vilotić, Aleksandra and Nedić, Olgica and Penezić, Ana",
year = "2023",
abstract = "Human transferrin (Tf) is a bilobal 76 kDa iron-binding glycoprotein present in human
serum. Each lobe has the ability to bind one ferric ion (Fe3+) and a single synergistic
bicarbonate anion. The main role of Tf is to transport Fe3+ ions through the circulation to
cells, via interaction with transferrin receptor (TFR) on the cell surface. Previously
described methods for Tf isolation and purification are either very time-consuming or
provide Tf of lower final purity. Here we describe a fast and simple FPLC method for the
isolation and purification of Tf from human serum. Serum samples were prepared by
precipitation, while protein purification was performed on FPLC system, using an anionexchange
column. Several different buffers at the same pH were tested. Tf purified by this
method was analyzed by Western blot, followed by immunodetection, as well as with
silver staining after SDS PAGE. Its functionality was tested with respect to iron-binding
capacity (ferozzine method) and its ability to interact with TFR by immunofluorescent
staining. The conformation of purified Tf was analyzed by recording intrinsic fluorescent
emmision spectra originating from Trp residues. The method itself is highly reproducible
(intra- and interday), easy to perform (only two steps) and fast (under an hour), yielding
98% to 99% pure Tf with all buffers. Purified Tf was shown to have retained its ironbinding
capacity, as well as the ability to interact with TFR. Purified Tf also retained its
native three-dimensional structure. Described method for the isolation and purification of
Tf is fast, simple and highly reproducible, yielding a functional Tf of high purity in its
native state while offering the flexibility of using different buffer systems. All of these
features make this protocol a method of choice for the isolation and purification of Tf on a
semi-preparative scale.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia",
title = "Simple two-step semi-preparative isolation and purification of transferrin from human serum",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_cer_6663"
}

Četić, D., Miljuš, G., Dobrijević, Z., Gligorijević, N., Vilotić, A., Nedić, O.,& Penezić, A.. (2023). Simple two-step semi-preparative isolation and purification of transferrin from human serum. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
Serbian Biochemical Society., 66-66.
https://hdl.handle.net/21.15107/rcub_cer_6663

Četić D, Miljuš G, Dobrijević Z, Gligorijević N, Vilotić A, Nedić O, Penezić A. Simple two-step semi-preparative isolation and purification of transferrin from human serum. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:66-66.
https://hdl.handle.net/21.15107/rcub_cer_6663 .

Četić, Danilo, Miljuš, Goran, Dobrijević, Zorana, Gligorijević, Nikola, Vilotić, Aleksandra, Nedić, Olgica, Penezić, Ana, "Simple two-step semi-preparative isolation and purification of transferrin from human serum" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):66-66,
https://hdl.handle.net/21.15107/rcub_cer_6663 .

TY  - CONF
AU  - Miljuš, Goran
AU  - Penezić, Ana
AU  - Noe, Dragana
AU  - Dobrijević, Zorana
AU  - Baralić, Marko
AU  - Robajac, Dragana
AU  - Šunderić, Miloš
AU  - Gligorijević, Nikola
AU  - Dimitrijević, Ivan
AU  - Barišić, Goran
AU  - Nedić, Olgica
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6664
AB  - Human serum tansferrin (hsTf) is a major circulatory protein crucial for the
transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target
tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks
of (patho)physiological conditions, alters protein structure and function. The main role of
hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions
contribute to diverse functions. The aim of this study was to profile interacting partners of
hsTf in the samples of serum coming from patients diagnosed with a wide range of
pathological conditions with underlying OS status. Anti-hsTf pull-down samples were
analysed using mass spectrometry. Data went through analysis by appropriate
bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in
sample groups of patients suffering from kidney insufficiency subjected to dialysis
treatment (peritoneal-PD or hemo-HD) also with patients with gestational diabetes
compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage
shows an inverse distribution of expression profiles in comparison to healthy samples.
Most prominent differences are seen in hsTf interacting partners involved in the
complement and coagulation cascades and cholesterol metabolic pathways, suggesting a
multifaceted role of hsTf in these processes throughout the course of the disease.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
T1  - Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_cer_6664
ER  - 

@conference{
author = "Miljuš, Goran and Penezić, Ana and Noe, Dragana and Dobrijević, Zorana and Baralić, Marko and Robajac, Dragana and Šunderić, Miloš and Gligorijević, Nikola and Dimitrijević, Ivan and Barišić, Goran and Nedić, Olgica",
year = "2023",
abstract = "Human serum tansferrin (hsTf) is a major circulatory protein crucial for the
transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target
tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks
of (patho)physiological conditions, alters protein structure and function. The main role of
hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions
contribute to diverse functions. The aim of this study was to profile interacting partners of
hsTf in the samples of serum coming from patients diagnosed with a wide range of
pathological conditions with underlying OS status. Anti-hsTf pull-down samples were
analysed using mass spectrometry. Data went through analysis by appropriate
bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in
sample groups of patients suffering from kidney insufficiency subjected to dialysis
treatment (peritoneal-PD or hemo-HD) also with patients with gestational diabetes
compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage
shows an inverse distribution of expression profiles in comparison to healthy samples.
Most prominent differences are seen in hsTf interacting partners involved in the
complement and coagulation cascades and cholesterol metabolic pathways, suggesting a
multifaceted role of hsTf in these processes throughout the course of the disease.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia",
title = "Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_cer_6664"
}

Miljuš, G., Penezić, A., Noe, D., Dobrijević, Z., Baralić, M., Robajac, D., Šunderić, M., Gligorijević, N., Dimitrijević, I., Barišić, G.,& Nedić, O.. (2023). Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
Serbian Biochemical Society., 69-69.
https://hdl.handle.net/21.15107/rcub_cer_6664

Miljuš G, Penezić A, Noe D, Dobrijević Z, Baralić M, Robajac D, Šunderić M, Gligorijević N, Dimitrijević I, Barišić G, Nedić O. Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:69-69.
https://hdl.handle.net/21.15107/rcub_cer_6664 .

Miljuš, Goran, Penezić, Ana, Noe, Dragana, Dobrijević, Zorana, Baralić, Marko, Robajac, Dragana, Šunderić, Miloš, Gligorijević, Nikola, Dimitrijević, Ivan, Barišić, Goran, Nedić, Olgica, "Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):69-69,
https://hdl.handle.net/21.15107/rcub_cer_6664 .

TY  - CONF
AU  - Nedić, Olgica
AU  - Dekanski, Aleksandar
AU  - Mrowinski, Maciej
AU  - Agata, Fronczak
AU  - Fronczak, Piotr
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6209
AB  - Анализирани су подаци из базе података часописа Journal of the Serbian Chemical Society који се односе на поднете радове у периоду 2015-2016.1 Већина приспелих радова је била из Индије, Ирана и Кине (укупно 49 %), док су радови из Србије били у мањини (12 %). Велики број радова је одбијен из техничких разлога. Укупно 1010 поднетих радова је послато 2089 пута. Само 279 радова (28 %) је прослеђено уредницима. 78 % аутора је поднело само један рад, 18 % два и 3 % три рада. Процес рецензирања за ауторе из Србије траје краће него за иностранe ауторе и већа је вероватноћа прихватања рада. Међу иностраним ауторима је више мушкараца, а међу домаћим жена. Вероватноћа прихватања рада је већа за женске него за мушке ауторе. Просечан број аутора из Србије на једном раду је 5, док је просечан број иностраних аутора по раду 2.
AB  - A dataset which contains information on the submitted manuscripts in the Journal of  the Serbian Chemical Society in the period 2015-2016 was evaluated.
PB  - Srpsko hemijsko društvo
C3  - Kratki izvodi radova, knjiga radova - 59. Savetovanje Srpskog hemijskog društva, 1. i 2. jun 2023. godine, Novi Sad / Book of Abstracts, Proceedings - 59th Meeting of the Serbian Chemical Society, June 1-2, 2023, Novi Sad, Serbia
T1  - Kо публикује у часопису Journal of the Serbian Chemical Society?
T1  - Who is publishing in the Journal of the Serbian Chemical Society?
SP  - 81
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_cer_6209
ER  - 

@conference{
author = "Nedić, Olgica and Dekanski, Aleksandar and Mrowinski, Maciej and Agata, Fronczak and Fronczak, Piotr",
year = "2023",
abstract = "Анализирани су подаци из базе података часописа Journal of the Serbian Chemical Society који се односе на поднете радове у периоду 2015-2016.1 Већина приспелих радова је била из Индије, Ирана и Кине (укупно 49 %), док су радови из Србије били у мањини (12 %). Велики број радова је одбијен из техничких разлога. Укупно 1010 поднетих радова је послато 2089 пута. Само 279 радова (28 %) је прослеђено уредницима. 78 % аутора је поднело само један рад, 18 % два и 3 % три рада. Процес рецензирања за ауторе из Србије траје краће него за иностранe ауторе и већа је вероватноћа прихватања рада. Међу иностраним ауторима је више мушкараца, а међу домаћим жена. Вероватноћа прихватања рада је већа за женске него за мушке ауторе. Просечан број аутора из Србије на једном раду је 5, док је просечан број иностраних аутора по раду 2., A dataset which contains information on the submitted manuscripts in the Journal of  the Serbian Chemical Society in the period 2015-2016 was evaluated.",
publisher = "Srpsko hemijsko društvo",
journal = "Kratki izvodi radova, knjiga radova - 59. Savetovanje Srpskog hemijskog društva, 1. i 2. jun 2023. godine, Novi Sad / Book of Abstracts, Proceedings - 59th Meeting of the Serbian Chemical Society, June 1-2, 2023, Novi Sad, Serbia",
title = "Kо публикује у часопису Journal of the Serbian Chemical Society?, Who is publishing in the Journal of the Serbian Chemical Society?",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_cer_6209"
}

Nedić, O., Dekanski, A., Mrowinski, M., Agata, F.,& Fronczak, P.. (2023). Kо публикује у часопису Journal of the Serbian Chemical Society?. in Kratki izvodi radova, knjiga radova - 59. Savetovanje Srpskog hemijskog društva, 1. i 2. jun 2023. godine, Novi Sad / Book of Abstracts, Proceedings - 59th Meeting of the Serbian Chemical Society, June 1-2, 2023, Novi Sad, Serbia
Srpsko hemijsko društvo., 81-81.
https://hdl.handle.net/21.15107/rcub_cer_6209

Nedić O, Dekanski A, Mrowinski M, Agata F, Fronczak P. Kо публикује у часопису Journal of the Serbian Chemical Society?. in Kratki izvodi radova, knjiga radova - 59. Savetovanje Srpskog hemijskog društva, 1. i 2. jun 2023. godine, Novi Sad / Book of Abstracts, Proceedings - 59th Meeting of the Serbian Chemical Society, June 1-2, 2023, Novi Sad, Serbia. 2023;:81-81.
https://hdl.handle.net/21.15107/rcub_cer_6209 .

Nedić, Olgica, Dekanski, Aleksandar, Mrowinski, Maciej, Agata, Fronczak, Fronczak, Piotr, "Kо публикује у часопису Journal of the Serbian Chemical Society?" in Kratki izvodi radova, knjiga radova - 59. Savetovanje Srpskog hemijskog društva, 1. i 2. jun 2023. godine, Novi Sad / Book of Abstracts, Proceedings - 59th Meeting of the Serbian Chemical Society, June 1-2, 2023, Novi Sad, Serbia (2023):81-81,
https://hdl.handle.net/21.15107/rcub_cer_6209 .

TY  - JOUR
AU  - Baralić, Marko
AU  - Robajac, Dragana
AU  - Penezić, Ana
AU  - Brković, Voin
AU  - Gligorijević, Nikola
AU  - Bontić, Ana
AU  - Pavlović, Jelena
AU  - Nikolić, Jelena
AU  - Miljuš, Goran
AU  - Dobrijević, Zorana
AU  - Šunderić, Miloš
AU  - Pažitna, Lucija
AU  - Katrlík, Jaroslav
AU  - Nedić, Olgica
AU  - Laušević, Mirjana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6445
AB  - In previous publications, we pointed out the importance of mannosylation of fibrinogen for the development of cardiovascular complications and fucosylation as a predictor of peritoneal membrane dysfunction in patients on peritoneal dialysis (PD). After a follow-up period of 30 months from the onset of the COVID-19 pandemic, we evaluated the significance of 1,25-dihydroxyvitamin D3 (calcitriol) therapy, primary disease, biochemical and hematologic analyzes, and previously performed glycan analysis by lectin-based microarray as predictors of mortality in this patient group. After univariate Cox regression analysis, diabetes mellitus (DM) and calcitriol therapy were found to be potential predictors of mortality. Additional multivariate Cox regression analysis confirmed that only DM was a predictor of mortality. Nevertheless, the use of calcitriol in therapy significantly reduced mortality in this patient group, as shown by the Kaplan–Meier survival curve. The presence of DM as a concomitant disease proved to be a strong predictor of fatal outcome in PD patients infected with SARS-CoV-2. This is the first study to indicate the importance and beneficial effect of calcitriol therapy on survival in PD patients with COVID-19 infection. In addition, this study points to the possibility that adverse thrombogenic events observed in PD patients during the pandemic may be caused by aberrant fibrinogen glycosylation.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Nutrients
T1  - Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19
VL  - 15
IS  - 9
SP  - 2050
DO  - 10.3390/nu15092050
ER  - 

@article{
author = "Baralić, Marko and Robajac, Dragana and Penezić, Ana and Brković, Voin and Gligorijević, Nikola and Bontić, Ana and Pavlović, Jelena and Nikolić, Jelena and Miljuš, Goran and Dobrijević, Zorana and Šunderić, Miloš and Pažitna, Lucija and Katrlík, Jaroslav and Nedić, Olgica and Laušević, Mirjana",
year = "2023",
abstract = "In previous publications, we pointed out the importance of mannosylation of fibrinogen for the development of cardiovascular complications and fucosylation as a predictor of peritoneal membrane dysfunction in patients on peritoneal dialysis (PD). After a follow-up period of 30 months from the onset of the COVID-19 pandemic, we evaluated the significance of 1,25-dihydroxyvitamin D3 (calcitriol) therapy, primary disease, biochemical and hematologic analyzes, and previously performed glycan analysis by lectin-based microarray as predictors of mortality in this patient group. After univariate Cox regression analysis, diabetes mellitus (DM) and calcitriol therapy were found to be potential predictors of mortality. Additional multivariate Cox regression analysis confirmed that only DM was a predictor of mortality. Nevertheless, the use of calcitriol in therapy significantly reduced mortality in this patient group, as shown by the Kaplan–Meier survival curve. The presence of DM as a concomitant disease proved to be a strong predictor of fatal outcome in PD patients infected with SARS-CoV-2. This is the first study to indicate the importance and beneficial effect of calcitriol therapy on survival in PD patients with COVID-19 infection. In addition, this study points to the possibility that adverse thrombogenic events observed in PD patients during the pandemic may be caused by aberrant fibrinogen glycosylation.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Nutrients",
title = "Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19",
volume = "15",
number = "9",
pages = "2050",
doi = "10.3390/nu15092050"
}

Baralić, M., Robajac, D., Penezić, A., Brković, V., Gligorijević, N., Bontić, A., Pavlović, J., Nikolić, J., Miljuš, G., Dobrijević, Z., Šunderić, M., Pažitna, L., Katrlík, J., Nedić, O.,& Laušević, M.. (2023). Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19. in Nutrients
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 15(9), 2050.
https://doi.org/10.3390/nu15092050

Baralić M, Robajac D, Penezić A, Brković V, Gligorijević N, Bontić A, Pavlović J, Nikolić J, Miljuš G, Dobrijević Z, Šunderić M, Pažitna L, Katrlík J, Nedić O, Laušević M. Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19. in Nutrients. 2023;15(9):2050.
doi:10.3390/nu15092050 .

Baralić, Marko, Robajac, Dragana, Penezić, Ana, Brković, Voin, Gligorijević, Nikola, Bontić, Ana, Pavlović, Jelena, Nikolić, Jelena, Miljuš, Goran, Dobrijević, Zorana, Šunderić, Miloš, Pažitna, Lucija, Katrlík, Jaroslav, Nedić, Olgica, Laušević, Mirjana, "Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19" in Nutrients, 15, no. 9 (2023):2050,
https://doi.org/10.3390/nu15092050 . .

TY  - JOUR
AU  - Baralić, Marko
AU  - Pažitná, Lucia
AU  - Brković, Voin
AU  - Laušević, Mirjana
AU  - Gligorijević, Nikola
AU  - Katrlík, Jaroslav
AU  - Nedić, Olgica
AU  - Robajac, Dragana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5664
AB  - As we already reported, fibrinogen fucosylation emerged as a prognostic marker of peritoneal
membrane function in end-stage renal disease (ESRD) patients on peritoneal dialysis. After a
follow-up period of 18 months, we estimated the ability of employed lectins, as well as other biochemical
parameters, to serve as mortality predictors in these patients. Following a univariate Cox
regression analysis, ferritin, urea clearance, residual diuresis, hyperglycemia, and an increase in the
signal intensity obtained with Galanthus nivalis lectin (GNL) emerged as potential mortality predictors,
but additional multivariate Cox regression analysis pointed only to glucose concentration
and GNL as mortality predictors. Higher signal intensity obtained with GNL in patients that died
suggested the importance of paucimannosidic/highly mannosidic N-glycan structures on fibrinogen
as factors that are related to unwanted cardiovascular events and all-cause mortality and can possibly
be seen as a prediction tool. Altered glycan structures composed of mannose residues are expected
to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly
the entire cascade of events linked to this lectin. Since patients with ESRD are prone to cardiovascular
complications and the formation of atherosclerotic plaques, one can hypothesize that fibrinogen
with increasingly exposed mannose residues may contribute to the unwanted events.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Cells
T1  - Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation
VL  - 12
IS  - 351
DO  - 10.3390/cells12030351
ER  - 

@article{
author = "Baralić, Marko and Pažitná, Lucia and Brković, Voin and Laušević, Mirjana and Gligorijević, Nikola and Katrlík, Jaroslav and Nedić, Olgica and Robajac, Dragana",
year = "2023",
abstract = "As we already reported, fibrinogen fucosylation emerged as a prognostic marker of peritoneal
membrane function in end-stage renal disease (ESRD) patients on peritoneal dialysis. After a
follow-up period of 18 months, we estimated the ability of employed lectins, as well as other biochemical
parameters, to serve as mortality predictors in these patients. Following a univariate Cox
regression analysis, ferritin, urea clearance, residual diuresis, hyperglycemia, and an increase in the
signal intensity obtained with Galanthus nivalis lectin (GNL) emerged as potential mortality predictors,
but additional multivariate Cox regression analysis pointed only to glucose concentration
and GNL as mortality predictors. Higher signal intensity obtained with GNL in patients that died
suggested the importance of paucimannosidic/highly mannosidic N-glycan structures on fibrinogen
as factors that are related to unwanted cardiovascular events and all-cause mortality and can possibly
be seen as a prediction tool. Altered glycan structures composed of mannose residues are expected
to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly
the entire cascade of events linked to this lectin. Since patients with ESRD are prone to cardiovascular
complications and the formation of atherosclerotic plaques, one can hypothesize that fibrinogen
with increasingly exposed mannose residues may contribute to the unwanted events.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Cells",
title = "Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation",
volume = "12",
number = "351",
doi = "10.3390/cells12030351"
}

Baralić, M., Pažitná, L., Brković, V., Laušević, M., Gligorijević, N., Katrlík, J., Nedić, O.,& Robajac, D.. (2023). Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation. in Cells
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 12(351).
https://doi.org/10.3390/cells12030351

Baralić M, Pažitná L, Brković V, Laušević M, Gligorijević N, Katrlík J, Nedić O, Robajac D. Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation. in Cells. 2023;12(351).
doi:10.3390/cells12030351 .

Baralić, Marko, Pažitná, Lucia, Brković, Voin, Laušević, Mirjana, Gligorijević, Nikola, Katrlík, Jaroslav, Nedić, Olgica, Robajac, Dragana, "Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation" in Cells, 12, no. 351 (2023),
https://doi.org/10.3390/cells12030351 . .

TY  - CONF
AU  - Nedić, Olgica
AU  - Gligorijević, Nikola
AU  - Penezić, Ana
AU  - Minić, Simeon
AU  - Radomirović, Mirjana
AU  - Nikolić, Milan
AU  - Ćirković Veličković, Tanja
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6728
AB  - Our research work was focused on interactions between resveratrol (R) and tibnnogen (I), (dihydro)alpha-lipoic acid (ALA) and fibrinogen or albumin, and phycocyanobilin (PCB) and catalase. Resveratrol is found in grapes and berries, leafy greens are a source of ALA and alga Spirulina is a source of PCB. L-P interactions were investigated by following-up structural changes of proteins and/or ligands using spectrometric methods (spectrofluorimetry, CD, FTIR) and by examining the primary role of individual proteins upon ligand binding.
PB  - Belgrade : University of Belgrade - Faculty of Agriculture
C3  - Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1EuSPMF), 7-9 September 2022, Belgrade, Serbia
T1  - Food antioxidants and their interaction with human proteins
SP  - 13
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_cer_6728
ER  - 

@conference{
author = "Nedić, Olgica and Gligorijević, Nikola and Penezić, Ana and Minić, Simeon and Radomirović, Mirjana and Nikolić, Milan and Ćirković Veličković, Tanja",
year = "2022",
abstract = "Our research work was focused on interactions between resveratrol (R) and tibnnogen (I), (dihydro)alpha-lipoic acid (ALA) and fibrinogen or albumin, and phycocyanobilin (PCB) and catalase. Resveratrol is found in grapes and berries, leafy greens are a source of ALA and alga Spirulina is a source of PCB. L-P interactions were investigated by following-up structural changes of proteins and/or ligands using spectrometric methods (spectrofluorimetry, CD, FTIR) and by examining the primary role of individual proteins upon ligand binding.",
publisher = "Belgrade : University of Belgrade - Faculty of Agriculture",
journal = "Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1EuSPMF), 7-9 September 2022, Belgrade, Serbia",
title = "Food antioxidants and their interaction with human proteins",
pages = "13-13",
url = "https://hdl.handle.net/21.15107/rcub_cer_6728"
}

Nedić, O., Gligorijević, N., Penezić, A., Minić, S., Radomirović, M., Nikolić, M.,& Ćirković Veličković, T.. (2022). Food antioxidants and their interaction with human proteins. in Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1EuSPMF), 7-9 September 2022, Belgrade, Serbia
Belgrade : University of Belgrade - Faculty of Agriculture., 13-13.
https://hdl.handle.net/21.15107/rcub_cer_6728

Nedić O, Gligorijević N, Penezić A, Minić S, Radomirović M, Nikolić M, Ćirković Veličković T. Food antioxidants and their interaction with human proteins. in Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1EuSPMF), 7-9 September 2022, Belgrade, Serbia. 2022;:13-13.
https://hdl.handle.net/21.15107/rcub_cer_6728 .

Nedić, Olgica, Gligorijević, Nikola, Penezić, Ana, Minić, Simeon, Radomirović, Mirjana, Nikolić, Milan, Ćirković Veličković, Tanja, "Food antioxidants and their interaction with human proteins" in Book of abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1EuSPMF), 7-9 September 2022, Belgrade, Serbia (2022):13-13,
https://hdl.handle.net/21.15107/rcub_cer_6728 .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Šunderić, Miloš
AU  - Minić, Simeon
AU  - Nedić, Olgica
AU  - Nikolić, Milan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6822
AB  - The interaction between phycocyanobilin (PCB), a bioactive
chromophore of blue-green cyanobacteria Spirulina’s phycobiliproteins,
and human alpha-2-macroglobulin (a2M), a universal
anti-proteinase, was investigated in this study under simulated
physiological conditions using spectroscopic techniques and a2M
activity assay. Protein a2M was found to bind PCB with a moderate
affinity, as assessed by spectrofluorimetric titration. The
binding constant was calculated to be 6.39105 M
 1 at 25°C. The
binding of PCB to a2M did not cause significant change in the
secondary structure of the protein, as determined by circular
dichroism. PCB protected a2M from oxidative damage in the
presence of AAPH-induced free radical overproduction. PCB
binding also effectively preserved a2M anti-proteinase activity.
Since a2M is involved in controlling the action of enzymes during
the inflammatory process, the protection that PCB expresses
could indirectly influence the intensity and direction of the body
response to impaired homeostasis, especially under oxidative
stress.
PB  - Wiley
C3  - FEBS Open Bio
T1  - Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications
VL  - 12
IS  - Supplement 1
SP  - 304
EP  - 304
DO  - 10.1002/2211-5463.13440
ER  - 

@conference{
author = "Gligorijević, Nikola and Šunderić, Miloš and Minić, Simeon and Nedić, Olgica and Nikolić, Milan",
year = "2022",
abstract = "The interaction between phycocyanobilin (PCB), a bioactive
chromophore of blue-green cyanobacteria Spirulina’s phycobiliproteins,
and human alpha-2-macroglobulin (a2M), a universal
anti-proteinase, was investigated in this study under simulated
physiological conditions using spectroscopic techniques and a2M
activity assay. Protein a2M was found to bind PCB with a moderate
affinity, as assessed by spectrofluorimetric titration. The
binding constant was calculated to be 6.39105 M
 1 at 25°C. The
binding of PCB to a2M did not cause significant change in the
secondary structure of the protein, as determined by circular
dichroism. PCB protected a2M from oxidative damage in the
presence of AAPH-induced free radical overproduction. PCB
binding also effectively preserved a2M anti-proteinase activity.
Since a2M is involved in controlling the action of enzymes during
the inflammatory process, the protection that PCB expresses
could indirectly influence the intensity and direction of the body
response to impaired homeostasis, especially under oxidative
stress.",
publisher = "Wiley",
journal = "FEBS Open Bio",
title = "Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications",
volume = "12",
number = "Supplement 1",
pages = "304-304",
doi = "10.1002/2211-5463.13440"
}

Gligorijević, N., Šunderić, M., Minić, S., Nedić, O.,& Nikolić, M.. (2022). Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications. in FEBS Open Bio
Wiley., 12(Supplement 1), 304-304.
https://doi.org/10.1002/2211-5463.13440

Gligorijević N, Šunderić M, Minić S, Nedić O, Nikolić M. Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications. in FEBS Open Bio. 2022;12(Supplement 1):304-304.
doi:10.1002/2211-5463.13440 .

Gligorijević, Nikola, Šunderić, Miloš, Minić, Simeon, Nedić, Olgica, Nikolić, Milan, "Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications" in FEBS Open Bio, 12, no. Supplement 1 (2022):304-304,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Minić, Simeon
AU  - Radibratović, Milica
AU  - Papadimitriou, Vassiliki
AU  - Nedić, Olgica
AU  - Sotiroudis, Theodore G.
AU  - Nikolić, Milan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4226
AB  - Phycocyanobilin is a dark blue linear tetrapyrrole chromophore covalently attached to protein subunits of phycobiliproteins present in the light-harvesting complexes of the cyanobacteria Arthrospira platensis (Spirulina “superfood”). It shows exceptional health-promoting properties and emerging use in various fields of bioscience and industry. This study aims to examine the mutual impact of phycocyanobilin interactions with catalase, a life-essential antioxidant enzyme. Fluorescence quenching experiments demonstrated moderate binding (Ka of 3.9 × 104 M−1 at 25 °C; n = 0.89) (static type), while van't Hoff plot points to an enthalpically driven ligand binding (ΔG = −28.2 kJ mol−1; ΔH = −41.9 kJ mol−1). No significant changes in protein secondary structures (α-helix content ~22%) and thermal protein stability in terms of enzyme tetramer subunits (Tm ~ 64 °C) were detected upon ligand binding. Alterations in the tertiary catalase structure were found without adverse effects on enzyme activity (~2 × 106 IU/mL). The docking study results indicated that the ligand most likely binds to amino acid residues (Asn141, Arg 362, Tyr369 and Asn384) near the cavity between the enzyme homotetramer subunits not related to the active site. Finally, complex formation protects the pigment from free-radical induced oxidation (bleaching), suggesting possible prolongation of its half-life and bioactivity in vivo if bound to catalase.
PB  - Elsevier
T2  - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
T1  - Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity
VL  - 251
SP  - 119483
DO  - 10.1016/j.saa.2021.119483
ER  - 

@article{
author = "Gligorijević, Nikola and Minić, Simeon and Radibratović, Milica and Papadimitriou, Vassiliki and Nedić, Olgica and Sotiroudis, Theodore G. and Nikolić, Milan",
year = "2021",
abstract = "Phycocyanobilin is a dark blue linear tetrapyrrole chromophore covalently attached to protein subunits of phycobiliproteins present in the light-harvesting complexes of the cyanobacteria Arthrospira platensis (Spirulina “superfood”). It shows exceptional health-promoting properties and emerging use in various fields of bioscience and industry. This study aims to examine the mutual impact of phycocyanobilin interactions with catalase, a life-essential antioxidant enzyme. Fluorescence quenching experiments demonstrated moderate binding (Ka of 3.9 × 104 M−1 at 25 °C; n = 0.89) (static type), while van't Hoff plot points to an enthalpically driven ligand binding (ΔG = −28.2 kJ mol−1; ΔH = −41.9 kJ mol−1). No significant changes in protein secondary structures (α-helix content ~22%) and thermal protein stability in terms of enzyme tetramer subunits (Tm ~ 64 °C) were detected upon ligand binding. Alterations in the tertiary catalase structure were found without adverse effects on enzyme activity (~2 × 106 IU/mL). The docking study results indicated that the ligand most likely binds to amino acid residues (Asn141, Arg 362, Tyr369 and Asn384) near the cavity between the enzyme homotetramer subunits not related to the active site. Finally, complex formation protects the pigment from free-radical induced oxidation (bleaching), suggesting possible prolongation of its half-life and bioactivity in vivo if bound to catalase.",
publisher = "Elsevier",
journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",
title = "Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity",
volume = "251",
pages = "119483",
doi = "10.1016/j.saa.2021.119483"
}

Gligorijević, N., Minić, S., Radibratović, M., Papadimitriou, V., Nedić, O., Sotiroudis, T. G.,& Nikolić, M.. (2021). Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity. in Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 251, 119483.
https://doi.org/10.1016/j.saa.2021.119483

Gligorijević N, Minić S, Radibratović M, Papadimitriou V, Nedić O, Sotiroudis TG, Nikolić M. Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity. in Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2021;251:119483.
doi:10.1016/j.saa.2021.119483 .

Gligorijević, Nikola, Minić, Simeon, Radibratović, Milica, Papadimitriou, Vassiliki, Nedić, Olgica, Sotiroudis, Theodore G., Nikolić, Milan, "Nutraceutical phycocyanobilin binding to catalase protects the pigment from oxidation without affecting catalytic activity" in Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 251 (2021):119483,
https://doi.org/10.1016/j.saa.2021.119483 . .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Šukalović, Vladimir
AU  - Minić, Simeon
AU  - Miljuš, Goran
AU  - Nedić, Olgica
AU  - Penezić, Ana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4763
AB  - The binding of a popular food supplement and well-known antioxidant, dihydro-alpha-lipoic acid (DHLA) to human serum albumin (HSA) was characterised. The binding was monitored by several spectroscopic methods together with the molecular docking approach. HSA was able to bind DHLA with moderate affinity, 1.00±0.05×104 M-1. Spectroscopic data demonstrated that the preferential binding site for DHLA on HSA is IIA (Sudlow I). Both experimental and molecular docking analysis identified electrostatic (salt bridges) and hydrogen bonds as the key interactions involved in DHLA binding to HSA. Molecular docking confirmed that the Sudlow I site could accommodate DHLA and that the ligand is bound to the protein in a specific conformation. The molecular dynamic simulation showed that the formed complex is stable. Binding of DHLA does not affect the structure of the protein, but it thermally stabilises HSA. Bound DHLA had no effect on the susceptibility of HSA to trypsin digestion. Since DHLA is a commonly used food supplement, knowledge of its pharmacokinetics and pharmacodynamic properties in an organism is very important. This study further expands it by providing a detailed analysis of its interaction with HSA, the primary drug transporter in the circulation.
AB  - У раду су описане карктеристике везивања дихидро-липоинске киселине (DHLA), познатог суплемента у исхрани и антиоксиданса, за албумин из серума људи (HSA). Процес везивања је праћен применом већег броја спектроскопских метода и молекул-
ским моделовањем. HSA везује DHLA умереним афинитетом, 1,00±0,05×104 M-1. Спектроскопски резултати су показали да је везујуће место IIA (Sudlow I) главно место везивања DHLA за HSA. Експериментални, као и резултати молекулског моделовања, су идентификовали електростатичке (сони мостови) и водоничне везе као главне типове
интеракција. Резултати молекулског моделовања су потврдили да и место Sudlow I може везати DHLA, која је у том случају у специфичној конформацији. Симулација молекулске динамике је показала да је формирани комплекс стабилан. Везивање DHLA не
утиче на структуру протеина, али повећава његову термалну стабилност. Везани DHLA не утиче на подложност HSA трипсинској дигестији. Како је DHLA уобичајен суплемент у исхрани, знање о његовим фармакоконетичким и фармакодинамичким особинама је веома важно. Ово испитивање допуњује досадашња знања детаљном анализом интеракције DHLA са HSA, примарним транспортним протеином лекова у циркулацији.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Physicochemical characterisation of dihydro-alpha-lipoic acid interaction with human serum albumin by multi-spectroscopic and molecular modelling approaches
T1  - Физичко-хемијска карактеризација интеракције дихидро-липоинске киселине и албумина из серума људи применом мулти-спектроскопских метода и молекулског моделовања
VL  - 86
IS  - 9
SP  - 795
EP  - 807
DO  - 10.2298/JSC210420041G
ER  - 

@article{
author = "Gligorijević, Nikola and Šukalović, Vladimir and Minić, Simeon and Miljuš, Goran and Nedić, Olgica and Penezić, Ana",
year = "2021",
abstract = "The binding of a popular food supplement and well-known antioxidant, dihydro-alpha-lipoic acid (DHLA) to human serum albumin (HSA) was characterised. The binding was monitored by several spectroscopic methods together with the molecular docking approach. HSA was able to bind DHLA with moderate affinity, 1.00±0.05×104 M-1. Spectroscopic data demonstrated that the preferential binding site for DHLA on HSA is IIA (Sudlow I). Both experimental and molecular docking analysis identified electrostatic (salt bridges) and hydrogen bonds as the key interactions involved in DHLA binding to HSA. Molecular docking confirmed that the Sudlow I site could accommodate DHLA and that the ligand is bound to the protein in a specific conformation. The molecular dynamic simulation showed that the formed complex is stable. Binding of DHLA does not affect the structure of the protein, but it thermally stabilises HSA. Bound DHLA had no effect on the susceptibility of HSA to trypsin digestion. Since DHLA is a commonly used food supplement, knowledge of its pharmacokinetics and pharmacodynamic properties in an organism is very important. This study further expands it by providing a detailed analysis of its interaction with HSA, the primary drug transporter in the circulation., У раду су описане карктеристике везивања дихидро-липоинске киселине (DHLA), познатог суплемента у исхрани и антиоксиданса, за албумин из серума људи (HSA). Процес везивања је праћен применом већег броја спектроскопских метода и молекул-
ским моделовањем. HSA везује DHLA умереним афинитетом, 1,00±0,05×104 M-1. Спектроскопски резултати су показали да је везујуће место IIA (Sudlow I) главно место везивања DHLA за HSA. Експериментални, као и резултати молекулског моделовања, су идентификовали електростатичке (сони мостови) и водоничне везе као главне типове
интеракција. Резултати молекулског моделовања су потврдили да и место Sudlow I може везати DHLA, која је у том случају у специфичној конформацији. Симулација молекулске динамике је показала да је формирани комплекс стабилан. Везивање DHLA не
утиче на структуру протеина, али повећава његову термалну стабилност. Везани DHLA не утиче на подложност HSA трипсинској дигестији. Како је DHLA уобичајен суплемент у исхрани, знање о његовим фармакоконетичким и фармакодинамичким особинама је веома важно. Ово испитивање допуњује досадашња знања детаљном анализом интеракције DHLA са HSA, примарним транспортним протеином лекова у циркулацији.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Physicochemical characterisation of dihydro-alpha-lipoic acid interaction with human serum albumin by multi-spectroscopic and molecular modelling approaches, Физичко-хемијска карактеризација интеракције дихидро-липоинске киселине и албумина из серума људи применом мулти-спектроскопских метода и молекулског моделовања",
volume = "86",
number = "9",
pages = "795-807",
doi = "10.2298/JSC210420041G"
}

Gligorijević, N., Šukalović, V., Minić, S., Miljuš, G., Nedić, O.,& Penezić, A.. (2021). Physicochemical characterisation of dihydro-alpha-lipoic acid interaction with human serum albumin by multi-spectroscopic and molecular modelling approaches. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 86(9), 795-807.
https://doi.org/10.2298/JSC210420041G

Gligorijević N, Šukalović V, Minić S, Miljuš G, Nedić O, Penezić A. Physicochemical characterisation of dihydro-alpha-lipoic acid interaction with human serum albumin by multi-spectroscopic and molecular modelling approaches. in Journal of the Serbian Chemical Society. 2021;86(9):795-807.
doi:10.2298/JSC210420041G .

Gligorijević, Nikola, Šukalović, Vladimir, Minić, Simeon, Miljuš, Goran, Nedić, Olgica, Penezić, Ana, "Physicochemical characterisation of dihydro-alpha-lipoic acid interaction with human serum albumin by multi-spectroscopic and molecular modelling approaches" in Journal of the Serbian Chemical Society, 86, no. 9 (2021):795-807,
https://doi.org/10.2298/JSC210420041G . .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Minić, Simeon
AU  - Radomirović, Mirjana
AU  - Lević, Steva
AU  - Ćirković Veličković, Tanja
AU  - Nikolić, Milan
AU  - Nedić, Olgica
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7287
AB  - Fibrinogen is a plasma protein most susceptible to oxidation. Through this chemical
modification, fibrinogen acquires thrombogenic characteristics in different
pathophysiological conditions. Increased carbonyl content and reduced porosity impair the
degradation of formed fibrin mediated by plasmin. Fibrinogen is capable of interacting
with many proteins, ions, and small molecules. These interactions can modify the
functions of this protein. The discovery of new binding partners that may protect
fibrinogen from harmful oxidation and, thus, preserve its normal function is essential.
Some of the newly detected interactions between fibrinogen and small, natural bioactive
molecules, together with the influence of these interactions on the structure and function of
fibrinogen, will be presented in this text.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Ligand binding to fibrinogen influences its structure and function
SP  - 31
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_cer_7287
ER  - 

@conference{
author = "Gligorijević, Nikola and Minić, Simeon and Radomirović, Mirjana and Lević, Steva and Ćirković Veličković, Tanja and Nikolić, Milan and Nedić, Olgica",
year = "2021",
abstract = "Fibrinogen is a plasma protein most susceptible to oxidation. Through this chemical
modification, fibrinogen acquires thrombogenic characteristics in different
pathophysiological conditions. Increased carbonyl content and reduced porosity impair the
degradation of formed fibrin mediated by plasmin. Fibrinogen is capable of interacting
with many proteins, ions, and small molecules. These interactions can modify the
functions of this protein. The discovery of new binding partners that may protect
fibrinogen from harmful oxidation and, thus, preserve its normal function is essential.
Some of the newly detected interactions between fibrinogen and small, natural bioactive
molecules, together with the influence of these interactions on the structure and function of
fibrinogen, will be presented in this text.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Ligand binding to fibrinogen influences its structure and function",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_cer_7287"
}

Gligorijević, N., Minić, S., Radomirović, M., Lević, S., Ćirković Veličković, T., Nikolić, M.,& Nedić, O.. (2021). Ligand binding to fibrinogen influences its structure and function. in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
Serbian Biochemical Society., 31-31.
https://hdl.handle.net/21.15107/rcub_cer_7287

Gligorijević N, Minić S, Radomirović M, Lević S, Ćirković Veličković T, Nikolić M, Nedić O. Ligand binding to fibrinogen influences its structure and function. in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:31-31.
https://hdl.handle.net/21.15107/rcub_cer_7287 .

Gligorijević, Nikola, Minić, Simeon, Radomirović, Mirjana, Lević, Steva, Ćirković Veličković, Tanja, Nikolić, Milan, Nedić, Olgica, "Ligand binding to fibrinogen influences its structure and function" in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):31-31,
https://hdl.handle.net/21.15107/rcub_cer_7287 .

TY  - CONF
AU  - Šunderić, Miloš
AU  - Gligorijević, Nikola
AU  - Minić, Simeon
AU  - Nedić, Olgica
AU  - Nikolić, Milan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7289
AB  - In this study, the interaction between phycocyanobilin (PCB)1, a bioactive chromophore of
blue-green algae Spirulina's phycobiliproteins, and alpha-2-macroglobulin (α2M)2, a
universal anti-proteinase, was investigated under simulated physiological conditions using
spectroscopic techniques and α2M activity assay. Using spectrofluorimetric measurements,
we found that α2M binds PCB with a moderate affinity, with a binding constant of 6.3×
105 M−1 at 25°C. The binding of PCB to α2M does not cause any significant change in the
secondary structure of the protein (circular dichroism measurements). Besides, PCB
protects α2M from structural oxidative alterations under AAPH-induced free radical
overproduction. Further, PCB binding effectively preserves α2M anti-proteinase activity.
Since α2M is involved in controlling the action of enzymes during the inflammatory
process, the protection that PCB expresses could indirectly influence the intensity and
direction of body response to impaired homeostasis, especially under oxidative stress.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications
SP  - 163
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_cer_7289
ER  - 

@conference{
author = "Šunderić, Miloš and Gligorijević, Nikola and Minić, Simeon and Nedić, Olgica and Nikolić, Milan",
year = "2021",
abstract = "In this study, the interaction between phycocyanobilin (PCB)1, a bioactive chromophore of
blue-green algae Spirulina's phycobiliproteins, and alpha-2-macroglobulin (α2M)2, a
universal anti-proteinase, was investigated under simulated physiological conditions using
spectroscopic techniques and α2M activity assay. Using spectrofluorimetric measurements,
we found that α2M binds PCB with a moderate affinity, with a binding constant of 6.3×
105 M−1 at 25°C. The binding of PCB to α2M does not cause any significant change in the
secondary structure of the protein (circular dichroism measurements). Besides, PCB
protects α2M from structural oxidative alterations under AAPH-induced free radical
overproduction. Further, PCB binding effectively preserves α2M anti-proteinase activity.
Since α2M is involved in controlling the action of enzymes during the inflammatory
process, the protection that PCB expresses could indirectly influence the intensity and
direction of body response to impaired homeostasis, especially under oxidative stress.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications",
pages = "163-163",
url = "https://hdl.handle.net/21.15107/rcub_cer_7289"
}

Šunderić, M., Gligorijević, N., Minić, S., Nedić, O.,& Nikolić, M.. (2021). Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications. in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
Serbian Biochemical Society., 163-163.
https://hdl.handle.net/21.15107/rcub_cer_7289

Šunderić M, Gligorijević N, Minić S, Nedić O, Nikolić M. Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications. in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:163-163.
https://hdl.handle.net/21.15107/rcub_cer_7289 .

Šunderić, Miloš, Gligorijević, Nikola, Minić, Simeon, Nedić, Olgica, Nikolić, Milan, "Interaction between alpha-2-macroglobulin and phycocyanobilin – structural and physiological implications" in Serbian Biochemical Society Tenth Conference with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):163-163,
https://hdl.handle.net/21.15107/rcub_cer_7289 .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Minić, Simeon
AU  - Radomirović, Mirjana
AU  - Lević, Steva M.
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
AU  - Nedić, Olgica
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7288
AB  - Fibrinogen is a plasma protein that is highly susceptible to oxidation. Because of this chemical modification, fibrinogen acquires thrombogenic characteristics under different pathophysiological conditions. Increased carbonyl content and reduced porosity impair the degradation of formed fibrin mediated by plasmin. Fibrinogen is capable of interacting with many proteins, ions, and small molecules. These interactions can modify the functions of this protein. The discovery of new binding partners that may protect fibrinogen from harmful oxidation and, thus, preserve its normal function is essential. Some of the newly detected interactions between fibrinogen and small, natural bioactive molecules, together with the influence of these interactions on the structure and function of fibrinogen, will be presented in this text.
PB  - University of Novi Sad - Faculty of Sciences, Department of Biology
T2  - Biologia Serbica
T1  - Ligand binding to fibrinogen influences its structure and function
VL  - 43
IS  - 1
SP  - 24
EP  - 31
DO  - 10.5281/zenodo.5512285
ER  - 

@article{
author = "Gligorijević, Nikola and Minić, Simeon and Radomirović, Mirjana and Lević, Steva M. and Nikolić, Milan and Ćirković-Veličković, Tanja and Nedić, Olgica",
year = "2021",
abstract = "Fibrinogen is a plasma protein that is highly susceptible to oxidation. Because of this chemical modification, fibrinogen acquires thrombogenic characteristics under different pathophysiological conditions. Increased carbonyl content and reduced porosity impair the degradation of formed fibrin mediated by plasmin. Fibrinogen is capable of interacting with many proteins, ions, and small molecules. These interactions can modify the functions of this protein. The discovery of new binding partners that may protect fibrinogen from harmful oxidation and, thus, preserve its normal function is essential. Some of the newly detected interactions between fibrinogen and small, natural bioactive molecules, together with the influence of these interactions on the structure and function of fibrinogen, will be presented in this text.",
publisher = "University of Novi Sad - Faculty of Sciences, Department of Biology",
journal = "Biologia Serbica",
title = "Ligand binding to fibrinogen influences its structure and function",
volume = "43",
number = "1",
pages = "24-31",
doi = "10.5281/zenodo.5512285"
}

Gligorijević, N., Minić, S., Radomirović, M., Lević, S. M., Nikolić, M., Ćirković-Veličković, T.,& Nedić, O.. (2021). Ligand binding to fibrinogen influences its structure and function. in Biologia Serbica
University of Novi Sad - Faculty of Sciences, Department of Biology., 43(1), 24-31.
https://doi.org/10.5281/zenodo.5512285

Gligorijević N, Minić S, Radomirović M, Lević SM, Nikolić M, Ćirković-Veličković T, Nedić O. Ligand binding to fibrinogen influences its structure and function. in Biologia Serbica. 2021;43(1):24-31.
doi:10.5281/zenodo.5512285 .

Gligorijević, Nikola, Minić, Simeon, Radomirović, Mirjana, Lević, Steva M., Nikolić, Milan, Ćirković-Veličković, Tanja, Nedić, Olgica, "Ligand binding to fibrinogen influences its structure and function" in Biologia Serbica, 43, no. 1 (2021):24-31,
https://doi.org/10.5281/zenodo.5512285 . .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Šukalović, Vladimir
AU  - Penezić, Ana
AU  - Nedić, Olgica
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7279
AB  - A reduced form of the alpha-lipoic acid, dihydro-alpha-lipoic
acid (DHLA) is a potent, naturally occurring antioxidant that is
found in higher amounts in plants like spinach, broccoli, potatoes,
tomatoes, carrots, beets and rice. DHLA can be consumed
as a food supplement as well, at doses up to 600 mg/day. DHLA
has an inhibitory effect on coagulation as it can reduce concentrations
of some coagulation factors. This study investigated a
direct interaction between DHLA and fibrinogen, the main protein
in coagulation and hemostasis. DHLA binds fibrinogen with
a moderate straight. Calculated constant from spectrofluorimetric
titration for DHLA/fibrinogen complex was 104 M -1. Fibrinogen
stability remains the same with only marginal structural changes
in its secondary structure favouring more ordered molecular
organisation upon DHLA binding, as determined by Fourier transform
infrared spectroscopy. Coagulation assay showed that
fibrinogen with bound DHLA forms fibrin with thicker fibres, as
measured by coagulation assay and is protected from oxidation to
a certain extent. Docking analysis showed that DHLA may bind
fibrinogen in its D regions, which are directly involved in the fibrin
formation. Obtained results support beneficial effects of DHLA
on fibrinogen and consequently on coagulation process, suggesting
that DHLA supplementation may be indicated for persons with
an increased risk of developing thrombotic complications, particularly
those whose fibrin is characterised by increased oxidative
modification and formation of thinner and less porous fibres.
Although further investigation is needed, our results suggest that
DHLA in complex with fibrinogen can be located at the site of
injury where it may exert antioxidant effects.
PB  - Wiley
C3  - FEBS OpenBio
T1  - Dihydro-alpha-lipoic acid binds and protects fibrinogen from oxidation and affects fibrin formation
VL  - 11
IS  - Supplement 1
SP  - 182
EP  - 183
DO  - 10.1002/2211-5463.13205
ER  - 

@conference{
author = "Gligorijević, Nikola and Šukalović, Vladimir and Penezić, Ana and Nedić, Olgica",
year = "2021",
abstract = "A reduced form of the alpha-lipoic acid, dihydro-alpha-lipoic
acid (DHLA) is a potent, naturally occurring antioxidant that is
found in higher amounts in plants like spinach, broccoli, potatoes,
tomatoes, carrots, beets and rice. DHLA can be consumed
as a food supplement as well, at doses up to 600 mg/day. DHLA
has an inhibitory effect on coagulation as it can reduce concentrations
of some coagulation factors. This study investigated a
direct interaction between DHLA and fibrinogen, the main protein
in coagulation and hemostasis. DHLA binds fibrinogen with
a moderate straight. Calculated constant from spectrofluorimetric
titration for DHLA/fibrinogen complex was 104 M -1. Fibrinogen
stability remains the same with only marginal structural changes
in its secondary structure favouring more ordered molecular
organisation upon DHLA binding, as determined by Fourier transform
infrared spectroscopy. Coagulation assay showed that
fibrinogen with bound DHLA forms fibrin with thicker fibres, as
measured by coagulation assay and is protected from oxidation to
a certain extent. Docking analysis showed that DHLA may bind
fibrinogen in its D regions, which are directly involved in the fibrin
formation. Obtained results support beneficial effects of DHLA
on fibrinogen and consequently on coagulation process, suggesting
that DHLA supplementation may be indicated for persons with
an increased risk of developing thrombotic complications, particularly
those whose fibrin is characterised by increased oxidative
modification and formation of thinner and less porous fibres.
Although further investigation is needed, our results suggest that
DHLA in complex with fibrinogen can be located at the site of
injury where it may exert antioxidant effects.",
publisher = "Wiley",
journal = "FEBS OpenBio",
title = "Dihydro-alpha-lipoic acid binds and protects fibrinogen from oxidation and affects fibrin formation",
volume = "11",
number = "Supplement 1",
pages = "182-183",
doi = "10.1002/2211-5463.13205"
}

Gligorijević, N., Šukalović, V., Penezić, A.,& Nedić, O.. (2021). Dihydro-alpha-lipoic acid binds and protects fibrinogen from oxidation and affects fibrin formation. in FEBS OpenBio
Wiley., 11(Supplement 1), 182-183.
https://doi.org/10.1002/2211-5463.13205

Gligorijević N, Šukalović V, Penezić A, Nedić O. Dihydro-alpha-lipoic acid binds and protects fibrinogen from oxidation and affects fibrin formation. in FEBS OpenBio. 2021;11(Supplement 1):182-183.
doi:10.1002/2211-5463.13205 .

Gligorijević, Nikola, Šukalović, Vladimir, Penezić, Ana, Nedić, Olgica, "Dihydro-alpha-lipoic acid binds and protects fibrinogen from oxidation and affects fibrin formation" in FEBS OpenBio, 11, no. Supplement 1 (2021):182-183,
https://doi.org/10.1002/2211-5463.13205 . .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Radomirović, Mirjana
AU  - Rajković, Andreja
AU  - Nedić, Olgica
AU  - Ćirković Veličković, Tanja
PY  - 2021
UR  - https://ec.europa.eu/research/participants/documents/downloadPublic?documentIds=080166e5deeb546e&appId=PPGMS
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7284
AB  - The French paradox describes a lower incidence of cardiovascular problems despite a high intake of
saturated fats. This phenomenon was associated with higher consumption of red wine, only to be later discovered that the presence of several antioxidants, including resveratrol, are responsible for it. We investigated if resveratrol has a more direct role in protection from harmful oxidation and development of thrombosis, presumably through binding to important proteins of the blood coagulation process. Spectrofluorimetric analysis demonstrated binding of resveratrol to fibrinogen, the main protein in the coagulation process, which also has an important application as a food additive in making of fibrin gels. Various spectroscopic methods have demonstrated that binding of
resveratrol does not unfold or destabilize fibrinogen since both near and far-UV CD spectra as well as its melting temperature remained unchanged. A mutuallyprotective effect against the free radical-
induced oxidation of resveratrol and fibrinogen was found. The presence of fibrinogen caused a very
small masking effect of the antioxidative potential of resveratrol, measured by a reduction of
hexacyanoferrate (III), while greatly increasing its solubility in an aqueous environment, thus increasing potential bioavailability and activity of resveratrol in circulation. By direct interaction and protection of fibrinogen, resveratrol may serve as an important antioxidant for prevention of
thrombosis. The antioxidative effect of resveratrol may also protect and thus keep the desired characteristics of fibrinogen during the application of this protein as a food additive.
PB  - University of Belgrade - Faculty of Chemistry
C3  - FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia
T1  - Resveratrol and fibrinogen interactions
SP  - 15
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_cer_7284
ER  - 

@conference{
author = "Gligorijević, Nikola and Radomirović, Mirjana and Rajković, Andreja and Nedić, Olgica and Ćirković Veličković, Tanja",
year = "2021",
abstract = "The French paradox describes a lower incidence of cardiovascular problems despite a high intake of
saturated fats. This phenomenon was associated with higher consumption of red wine, only to be later discovered that the presence of several antioxidants, including resveratrol, are responsible for it. We investigated if resveratrol has a more direct role in protection from harmful oxidation and development of thrombosis, presumably through binding to important proteins of the blood coagulation process. Spectrofluorimetric analysis demonstrated binding of resveratrol to fibrinogen, the main protein in the coagulation process, which also has an important application as a food additive in making of fibrin gels. Various spectroscopic methods have demonstrated that binding of
resveratrol does not unfold or destabilize fibrinogen since both near and far-UV CD spectra as well as its melting temperature remained unchanged. A mutuallyprotective effect against the free radical-
induced oxidation of resveratrol and fibrinogen was found. The presence of fibrinogen caused a very
small masking effect of the antioxidative potential of resveratrol, measured by a reduction of
hexacyanoferrate (III), while greatly increasing its solubility in an aqueous environment, thus increasing potential bioavailability and activity of resveratrol in circulation. By direct interaction and protection of fibrinogen, resveratrol may serve as an important antioxidant for prevention of
thrombosis. The antioxidative effect of resveratrol may also protect and thus keep the desired characteristics of fibrinogen during the application of this protein as a food additive.",
publisher = "University of Belgrade - Faculty of Chemistry",
journal = "FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia",
title = "Resveratrol and fibrinogen interactions",
pages = "15-15",
url = "https://hdl.handle.net/21.15107/rcub_cer_7284"
}

Gligorijević, N., Radomirović, M., Rajković, A., Nedić, O.,& Ćirković Veličković, T.. (2021). Resveratrol and fibrinogen interactions. in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia
University of Belgrade - Faculty of Chemistry., 15-15.
https://hdl.handle.net/21.15107/rcub_cer_7284

Gligorijević N, Radomirović M, Rajković A, Nedić O, Ćirković Veličković T. Resveratrol and fibrinogen interactions. in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia. 2021;:15-15.
https://hdl.handle.net/21.15107/rcub_cer_7284 .

Gligorijević, Nikola, Radomirović, Mirjana, Rajković, Andreja, Nedić, Olgica, Ćirković Veličković, Tanja, "Resveratrol and fibrinogen interactions" in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Book of Abstracts, 16th-18th June, 2021, Belgrade, Serbia (2021):15-15,
https://hdl.handle.net/21.15107/rcub_cer_7284 .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Šukalović, Vladimir
AU  - Penezić, Ana
AU  - Nedić, Olgica
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3378
AB  - A reduced form of the alpha-lipoic acid, dihydro-alpha-lipoic acid (DHLA) is a potent, naturally occurring antioxidant which can be consumed as food constituent or as supplement at doses up to 600 mg/day. DHLA has inhibitory effect on coagulation as it can reduce concentrations of some coagulation factors. In this study, a direct interaction between DHLA and fibrinogen, the main protein in coagulation, is described. Binding constant for DHLA/fibrinogen complex is of moderate strength (104) and interaction probably occurs in D regions of fibrinogen, as shown by docking simulations. Fibrinogen stability remains the same with only marginal structural changes in its secondary structure favouring more ordered molecular organisation upon DHLA binding. Fibrinogen with bound DHLA forms fibrin with thicker fibers, as measured by coagulation assay and is protected from oxidation to certain extent. Obtained results support beneficial effects of DHLA on fibrinogen and consequently on coagulation process, suggesting that DHLA supplementation may be indicated for persons with an increased risk of developing thrombotic complications, particularly those whose fibrin is characterised by increased oxidative modification and formation of thinner and less porous fibers. Also, DHLA in complex with fibrinogen can be located at site of injury where it may exert antioxidant effects.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation
VL  - 147
SP  - 319
EP  - 325
DO  - 10.1016/j.ijbiomac.2020.01.098
ER  - 

@article{
author = "Gligorijević, Nikola and Šukalović, Vladimir and Penezić, Ana and Nedić, Olgica",
year = "2020",
abstract = "A reduced form of the alpha-lipoic acid, dihydro-alpha-lipoic acid (DHLA) is a potent, naturally occurring antioxidant which can be consumed as food constituent or as supplement at doses up to 600 mg/day. DHLA has inhibitory effect on coagulation as it can reduce concentrations of some coagulation factors. In this study, a direct interaction between DHLA and fibrinogen, the main protein in coagulation, is described. Binding constant for DHLA/fibrinogen complex is of moderate strength (104) and interaction probably occurs in D regions of fibrinogen, as shown by docking simulations. Fibrinogen stability remains the same with only marginal structural changes in its secondary structure favouring more ordered molecular organisation upon DHLA binding. Fibrinogen with bound DHLA forms fibrin with thicker fibers, as measured by coagulation assay and is protected from oxidation to certain extent. Obtained results support beneficial effects of DHLA on fibrinogen and consequently on coagulation process, suggesting that DHLA supplementation may be indicated for persons with an increased risk of developing thrombotic complications, particularly those whose fibrin is characterised by increased oxidative modification and formation of thinner and less porous fibers. Also, DHLA in complex with fibrinogen can be located at site of injury where it may exert antioxidant effects.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation",
volume = "147",
pages = "319-325",
doi = "10.1016/j.ijbiomac.2020.01.098"
}

Gligorijević, N., Šukalović, V., Penezić, A.,& Nedić, O.. (2020). Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation. in International Journal of Biological Macromolecules
Elsevier., 147, 319-325.
https://doi.org/10.1016/j.ijbiomac.2020.01.098

Gligorijević N, Šukalović V, Penezić A, Nedić O. Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation. in International Journal of Biological Macromolecules. 2020;147:319-325.
doi:10.1016/j.ijbiomac.2020.01.098 .

Gligorijević, Nikola, Šukalović, Vladimir, Penezić, Ana, Nedić, Olgica, "Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation" in International Journal of Biological Macromolecules, 147 (2020):319-325,
https://doi.org/10.1016/j.ijbiomac.2020.01.098 . .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Šukalović, Vladimir
AU  - Penezić, Ana
AU  - Nedić, Olgica
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3385
AB  - A reduced form of the alpha-lipoic acid, dihydro-alpha-lipoic acid (DHLA) is a potent, naturally occurring antioxidant which can be consumed as food constituent or as supplement at doses up to 600 mg/day. DHLA has inhibitory effect on coagulation as it can reduce concentrations of some coagulation factors. In this study, a direct interaction between DHLA and fibrinogen, the main protein in coagulation, is described. Binding constant for DHLA/fibrinogen complex is of moderate strength (104) and interaction probably occurs in D regions of fibrinogen, as shown by docking simulations. Fibrinogen stability remains the same with only marginal structural changes in its secondary structure favouring more ordered molecular organisation upon DHLA binding. Fibrinogen with bound DHLA forms fibrin with thicker fibers, as measured by coagulation assay and is protected from oxidation to certain extent. Obtained results support beneficial effects of DHLA on fibrinogen and consequently on coagulation process, suggesting that DHLA supplementation may be indicated for persons with an increased risk of developing thrombotic complications, particularly those whose fibrin is characterised by increased oxidative modification and formation of thinner and less porous fibers. Also, DHLA in complex with fibrinogen can be located at site of injury where it may exert antioxidant effects.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation
VL  - 147
SP  - 319
EP  - 325
DO  - 10.1016/j.ijbiomac.2020.01.098
ER  - 

@article{
author = "Gligorijević, Nikola and Šukalović, Vladimir and Penezić, Ana and Nedić, Olgica",
year = "2020",
abstract = "A reduced form of the alpha-lipoic acid, dihydro-alpha-lipoic acid (DHLA) is a potent, naturally occurring antioxidant which can be consumed as food constituent or as supplement at doses up to 600 mg/day. DHLA has inhibitory effect on coagulation as it can reduce concentrations of some coagulation factors. In this study, a direct interaction between DHLA and fibrinogen, the main protein in coagulation, is described. Binding constant for DHLA/fibrinogen complex is of moderate strength (104) and interaction probably occurs in D regions of fibrinogen, as shown by docking simulations. Fibrinogen stability remains the same with only marginal structural changes in its secondary structure favouring more ordered molecular organisation upon DHLA binding. Fibrinogen with bound DHLA forms fibrin with thicker fibers, as measured by coagulation assay and is protected from oxidation to certain extent. Obtained results support beneficial effects of DHLA on fibrinogen and consequently on coagulation process, suggesting that DHLA supplementation may be indicated for persons with an increased risk of developing thrombotic complications, particularly those whose fibrin is characterised by increased oxidative modification and formation of thinner and less porous fibers. Also, DHLA in complex with fibrinogen can be located at site of injury where it may exert antioxidant effects.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation",
volume = "147",
pages = "319-325",
doi = "10.1016/j.ijbiomac.2020.01.098"
}

Gligorijević, N., Šukalović, V., Penezić, A.,& Nedić, O.. (2020). Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation. in International Journal of Biological Macromolecules
Elsevier., 147, 319-325.
https://doi.org/10.1016/j.ijbiomac.2020.01.098

Gligorijević N, Šukalović V, Penezić A, Nedić O. Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation. in International Journal of Biological Macromolecules. 2020;147:319-325.
doi:10.1016/j.ijbiomac.2020.01.098 .

Gligorijević, Nikola, Šukalović, Vladimir, Penezić, Ana, Nedić, Olgica, "Characterisation of the binding of dihydro-alpha-lipoic acid to fibrinogen and the effects on fibrinogen oxidation and fibrin formation" in International Journal of Biological Macromolecules, 147 (2020):319-325,
https://doi.org/10.1016/j.ijbiomac.2020.01.098 . .

TY  - JOUR
AU  - Mrowinski, Maciej J.
AU  - Fronczak, Agata
AU  - Fronczak, Piotr
AU  - Nedić, Olgica
AU  - Dekanski, Aleksandar
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3683
AB  - In this paper, we provide insight into the editorial process as seen from the perspective
of journal editors. We study a dataset obtained from the Journal of the Serbian Chemical
Society, which contains information about submitted and rejected manuscripts, in order to
find differences between local (Serbian) and external (non-Serbian) submissions. We show
that external submissions (mainly from India, Iran and China) constitute the majority of all
submissions, while local submissions are in the minority. Most of submissions are rejected
for technical reasons (e.g. wrong manuscript formatting or problems with images) and
many users resubmit the same paper without making necessary corrections. Manuscripts
with just one author are less likely to pass the technical check, which can be attributed to
missing metadata. Articles from local authors are better prepared and require fewer resubmissions
on average before they are accepted for peer review. The peer review process for
local submissions takes less time than for external papers and local submissions are more
likely to be accepted for publication. Also, while there are more men than women among
external users, this trend is reversed for local users. In the combined group of local and
external users, articles submitted by women are more likely to be published than articles
submitted by men.
PB  - Netherlands : Springer
T2  - Scientometrics
T1  - The hurdles of academic publishing from the perspective of journal editors: a case study
VL  - 125
IS  - 1
SP  - 115
EP  - 133
DO  - 10.1007/s11192-020-03619-x
ER  - 

@article{
author = "Mrowinski, Maciej J. and Fronczak, Agata and Fronczak, Piotr and Nedić, Olgica and Dekanski, Aleksandar",
year = "2020",
abstract = "In this paper, we provide insight into the editorial process as seen from the perspective
of journal editors. We study a dataset obtained from the Journal of the Serbian Chemical
Society, which contains information about submitted and rejected manuscripts, in order to
find differences between local (Serbian) and external (non-Serbian) submissions. We show
that external submissions (mainly from India, Iran and China) constitute the majority of all
submissions, while local submissions are in the minority. Most of submissions are rejected
for technical reasons (e.g. wrong manuscript formatting or problems with images) and
many users resubmit the same paper without making necessary corrections. Manuscripts
with just one author are less likely to pass the technical check, which can be attributed to
missing metadata. Articles from local authors are better prepared and require fewer resubmissions
on average before they are accepted for peer review. The peer review process for
local submissions takes less time than for external papers and local submissions are more
likely to be accepted for publication. Also, while there are more men than women among
external users, this trend is reversed for local users. In the combined group of local and
external users, articles submitted by women are more likely to be published than articles
submitted by men.",
publisher = "Netherlands : Springer",
journal = "Scientometrics",
title = "The hurdles of academic publishing from the perspective of journal editors: a case study",
volume = "125",
number = "1",
pages = "115-133",
doi = "10.1007/s11192-020-03619-x"
}

Mrowinski, M. J., Fronczak, A., Fronczak, P., Nedić, O.,& Dekanski, A.. (2020). The hurdles of academic publishing from the perspective of journal editors: a case study. in Scientometrics
Netherlands : Springer., 125(1), 115-133.
https://doi.org/10.1007/s11192-020-03619-x

Mrowinski MJ, Fronczak A, Fronczak P, Nedić O, Dekanski A. The hurdles of academic publishing from the perspective of journal editors: a case study. in Scientometrics. 2020;125(1):115-133.
doi:10.1007/s11192-020-03619-x .

Mrowinski, Maciej J., Fronczak, Agata, Fronczak, Piotr, Nedić, Olgica, Dekanski, Aleksandar, "The hurdles of academic publishing from the perspective of journal editors: a case study" in Scientometrics, 125, no. 1 (2020):115-133,
https://doi.org/10.1007/s11192-020-03619-x . .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Šunderić, Miloš
AU  - Vilotić, Aleksandra
AU  - Baralić, Marko
AU  - Nedić, Olgica
PY  - 2019
UR  - http://www.bds.org.rs/download/SBS_Conference_09_2019.pdf
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7291
AB  - Alpha-2-macroglobulin (α2M) is a homotetrameric blood glycoprotein having molecular
mass of 720 kDa which acts as a general protease inhibitor 1. So far, the methods to
estimate the quantity of α2M and its activity were separate procedures. The quantity is
usually measured by immunochemical assays and the anti-protease activity of α2M by
measuring the activity of trypsin bound to α2M using chromogenic substrate BAPNA 2. A
simple and reliable method for determination of the concentration and function of α2M by
zymography was developed. This method is based on the covalent binding of α2M and
trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the
electrophoretic gel. The results have shown that α2M binds trypsin in a linear,
concentration-dependent manner. The sensitivity of the method is 125 nM with an intraassay
coefficient of variation 4.2 %. Freezing of α2M induces its partial denaturation,
which can be seen as the reduction in the amount of functional molecule and its reactivity
with trypsin. The method was further tested using α2M from patients with an end-stage
renal disease who are known to be under an increased oxidative stress and inflammation,
which are expected to modify the structure of proteins. Using α2M from these patients,
lower affinity of α2M towards trypsin was detected when compaired to α2M isolated from
healthy persons. The reported zymographic method enables measurement of α2M taking
into consideration both its quantity and function, stressing the importance of determination
of the amount of physiologically active molecules and not just their total amount present in
the sample. Monitoring of the relation quantity/activity becomes very important when the
sample originates from an individual exposed to a stress or with a disease accompanied by
post-translational modifications of proteins such as diabetes, renal disease or cancer 3.
Presented method also enables determination of α2M in the presence of different modifying
chemical substances.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia
T1  - Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography
SP  - 98
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_cer_7291
ER  - 

@conference{
author = "Gligorijević, Nikola and Šunderić, Miloš and Vilotić, Aleksandra and Baralić, Marko and Nedić, Olgica",
year = "2019",
abstract = "Alpha-2-macroglobulin (α2M) is a homotetrameric blood glycoprotein having molecular
mass of 720 kDa which acts as a general protease inhibitor 1. So far, the methods to
estimate the quantity of α2M and its activity were separate procedures. The quantity is
usually measured by immunochemical assays and the anti-protease activity of α2M by
measuring the activity of trypsin bound to α2M using chromogenic substrate BAPNA 2. A
simple and reliable method for determination of the concentration and function of α2M by
zymography was developed. This method is based on the covalent binding of α2M and
trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the
electrophoretic gel. The results have shown that α2M binds trypsin in a linear,
concentration-dependent manner. The sensitivity of the method is 125 nM with an intraassay
coefficient of variation 4.2 %. Freezing of α2M induces its partial denaturation,
which can be seen as the reduction in the amount of functional molecule and its reactivity
with trypsin. The method was further tested using α2M from patients with an end-stage
renal disease who are known to be under an increased oxidative stress and inflammation,
which are expected to modify the structure of proteins. Using α2M from these patients,
lower affinity of α2M towards trypsin was detected when compaired to α2M isolated from
healthy persons. The reported zymographic method enables measurement of α2M taking
into consideration both its quantity and function, stressing the importance of determination
of the amount of physiologically active molecules and not just their total amount present in
the sample. Monitoring of the relation quantity/activity becomes very important when the
sample originates from an individual exposed to a stress or with a disease accompanied by
post-translational modifications of proteins such as diabetes, renal disease or cancer 3.
Presented method also enables determination of α2M in the presence of different modifying
chemical substances.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia",
title = "Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_cer_7291"
}

Gligorijević, N., Šunderić, M., Vilotić, A., Baralić, M.,& Nedić, O.. (2019). Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography. in Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia
Serbian Biochemical Society., 98-98.
https://hdl.handle.net/21.15107/rcub_cer_7291

Gligorijević N, Šunderić M, Vilotić A, Baralić M, Nedić O. Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography. in Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia. 2019;:98-98.
https://hdl.handle.net/21.15107/rcub_cer_7291 .

Gligorijević, Nikola, Šunderić, Miloš, Vilotić, Aleksandra, Baralić, Marko, Nedić, Olgica, "Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography" in Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia (2019):98-98,
https://hdl.handle.net/21.15107/rcub_cer_7291 .

TY  - JOUR
AU  - Ausloos, Marcel
AU  - Nedić, Olgica
AU  - Dekanski, Aleksandar
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3038
AB  - This paper presents a novel method for finding features in the analysis of variable distributions stemming from time series. We apply the methodology to the case of submitted and accepted papers in peer-reviewed journals. We provide a comparative study of editorial decisions for papers submitted to two peer-reviewed journals: the Journal of the Serbian Chemical Society (JSCS) and this MDPI Entropy journal. We cover three recent years for which the fate of submitted papers-about 600 papers to JSCS and 2500 to Entropy-is completely determined. Instead of comparing the number distributions of these papers as a function of time with respect to a uniform distribution, we analyze the relevant probabilities, from which we derive the information entropy. It is argued that such probabilities are indeed more relevant for authors than the actual number of submissions. We tie this entropy analysis to the so called diversity of the variable distributions. Furthermore, we emphasize the correspondence between the entropy and the diversity with inequality measures, like the Herfindahl-Hirschman index and the Theil index, itself being in the class of entropy measures; the Gini coefficient which also measures the diversity in ranking is calculated for further discussion. In this sample, the seasonal aspects of the peer review process are outlined. It is found that the use of such indices, non linear transformations of the data distributions, allow us to distinguish features and evolutions of the peer review process as a function of time as well as comparing the non-uniformity of distributions. Furthermore, t- and z-statistical tests are applied in order to measure the significance (p-level) of the findings, that is, whether papers are more likely to be accepted if they are submitted during a few specific months or during a particular "season"; the predictability strength depends on the journal.
PB  - MDPI
T2  - Entropy
T1  - Seasonal Entropy, Diversity and Inequality Measures of Submitted and Accepted Papers Distributions in Peer-Reviewed Journals
VL  - 21
IS  - 6
SP  - 564
DO  - 10.3390/e21060564
ER  - 

@article{
author = "Ausloos, Marcel and Nedić, Olgica and Dekanski, Aleksandar",
year = "2019",
abstract = "This paper presents a novel method for finding features in the analysis of variable distributions stemming from time series. We apply the methodology to the case of submitted and accepted papers in peer-reviewed journals. We provide a comparative study of editorial decisions for papers submitted to two peer-reviewed journals: the Journal of the Serbian Chemical Society (JSCS) and this MDPI Entropy journal. We cover three recent years for which the fate of submitted papers-about 600 papers to JSCS and 2500 to Entropy-is completely determined. Instead of comparing the number distributions of these papers as a function of time with respect to a uniform distribution, we analyze the relevant probabilities, from which we derive the information entropy. It is argued that such probabilities are indeed more relevant for authors than the actual number of submissions. We tie this entropy analysis to the so called diversity of the variable distributions. Furthermore, we emphasize the correspondence between the entropy and the diversity with inequality measures, like the Herfindahl-Hirschman index and the Theil index, itself being in the class of entropy measures; the Gini coefficient which also measures the diversity in ranking is calculated for further discussion. In this sample, the seasonal aspects of the peer review process are outlined. It is found that the use of such indices, non linear transformations of the data distributions, allow us to distinguish features and evolutions of the peer review process as a function of time as well as comparing the non-uniformity of distributions. Furthermore, t- and z-statistical tests are applied in order to measure the significance (p-level) of the findings, that is, whether papers are more likely to be accepted if they are submitted during a few specific months or during a particular "season"; the predictability strength depends on the journal.",
publisher = "MDPI",
journal = "Entropy",
title = "Seasonal Entropy, Diversity and Inequality Measures of Submitted and Accepted Papers Distributions in Peer-Reviewed Journals",
volume = "21",
number = "6",
pages = "564",
doi = "10.3390/e21060564"
}

Ausloos, M., Nedić, O.,& Dekanski, A.. (2019). Seasonal Entropy, Diversity and Inequality Measures of Submitted and Accepted Papers Distributions in Peer-Reviewed Journals. in Entropy
MDPI., 21(6), 564.
https://doi.org/10.3390/e21060564

Ausloos M, Nedić O, Dekanski A. Seasonal Entropy, Diversity and Inequality Measures of Submitted and Accepted Papers Distributions in Peer-Reviewed Journals. in Entropy. 2019;21(6):564.
doi:10.3390/e21060564 .

Ausloos, Marcel, Nedić, Olgica, Dekanski, Aleksandar, "Seasonal Entropy, Diversity and Inequality Measures of Submitted and Accepted Papers Distributions in Peer-Reviewed Journals" in Entropy, 21, no. 6 (2019):564,
https://doi.org/10.3390/e21060564 . .

TY  - JOUR
AU  - Ausloos, Marcel
AU  - Nedić, Olgica
AU  - Dekanski, Aleksandar
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2641
AB  - This paper provides a comparative study about seasonal influence on editorial decisions for papers submitted to two peer review journals. We distinguish a specialized one, the Journal of the Serbian Chemical Society (JSCS) and an interdisciplinary one, Entropy. Dates of electronic submission for about 600 papers to JSCS and 2500 to Entropy have been recorded over 3 recent years. Time series of either accepted or rejected papers are subsequently analyzed. We take either editors or authors view points into account, thereby considering magnitudes and probabilities. In this sample, it is found that there are distinguishable peaks and dips in the time series, demonstrating preferred months for the submission of papers. It is also found that papers are more likely accepted if they are submitted during a few specific months—these depending on the journal. The probability of having a rejected paper also appears to be seasonally biased. In view of clarifying reports with contradictory findings, we discuss previously proposed conjectures for such effects, like holiday effects and the desk rejection by editors. We conclude that, in this sample, the type of journal, specialized or multidisciplinary, seems to be the drastic criterion for distinguishing the outcomes rates.
PB  - Springer Nature
T2  - Scientometrics
T2  - Scientometrics
T1  - Correlations between submission and acceptance of papers in peer review journals
VL  - 119
SP  - 279
EP  - 302
DO  - 10.1007/s11192-019-03026-x
ER  - 

@article{
author = "Ausloos, Marcel and Nedić, Olgica and Dekanski, Aleksandar",
year = "2019",
abstract = "This paper provides a comparative study about seasonal influence on editorial decisions for papers submitted to two peer review journals. We distinguish a specialized one, the Journal of the Serbian Chemical Society (JSCS) and an interdisciplinary one, Entropy. Dates of electronic submission for about 600 papers to JSCS and 2500 to Entropy have been recorded over 3 recent years. Time series of either accepted or rejected papers are subsequently analyzed. We take either editors or authors view points into account, thereby considering magnitudes and probabilities. In this sample, it is found that there are distinguishable peaks and dips in the time series, demonstrating preferred months for the submission of papers. It is also found that papers are more likely accepted if they are submitted during a few specific months—these depending on the journal. The probability of having a rejected paper also appears to be seasonally biased. In view of clarifying reports with contradictory findings, we discuss previously proposed conjectures for such effects, like holiday effects and the desk rejection by editors. We conclude that, in this sample, the type of journal, specialized or multidisciplinary, seems to be the drastic criterion for distinguishing the outcomes rates.",
publisher = "Springer Nature",
journal = "Scientometrics, Scientometrics",
title = "Correlations between submission and acceptance of papers in peer review journals",
volume = "119",
pages = "279-302",
doi = "10.1007/s11192-019-03026-x"
}

Ausloos, M., Nedić, O.,& Dekanski, A.. (2019). Correlations between submission and acceptance of papers in peer review journals. in Scientometrics
Springer Nature., 119, 279-302.
https://doi.org/10.1007/s11192-019-03026-x

Ausloos M, Nedić O, Dekanski A. Correlations between submission and acceptance of papers in peer review journals. in Scientometrics. 2019;119:279-302.
doi:10.1007/s11192-019-03026-x .

Ausloos, Marcel, Nedić, Olgica, Dekanski, Aleksandar, "Correlations between submission and acceptance of papers in peer review journals" in Scientometrics, 119 (2019):279-302,
https://doi.org/10.1007/s11192-019-03026-x . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Dekanski, Aleksandar
AU  - Budjevac, Nevena
AU  - Umeljic, Ivan
AU  - Nedić, Olgica
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3292
AB  - Velika brzina stvaranja naučnih informacija u poslednjih nekoliko godina nameće potrebu za povećanjem broja stručnih recenzenata, posebno što recenziranje dobija novu dimenziju važnosti i u svetlu rastućeg broja prepoznatih plagijata i izmisljenih rezultata. Potencijalno rešenje potrebe za povećanjem broja recenzenata je sistematska edukacija doktoranada i mladih istraživača, posebno u zemljama razvoju. Prema podacima sa četiri državna univerziteta u Srbiji (u Beogradu, Novom Sadu, Nišu i Kragujevcu) i jednog privatnog univerziteta (Univerzitet Singidunum), postoji niz doktorskih kurseva koji studente obrazuju o načinu sprovođenja istraživanja, nekoliko o tome kako da pišu naučne publikacije, ali samo jedan od njih skromno osposobljava studente za recenziranje naučnih radova. Dakle, U Srbiji ogromni potencijal doktoranada i mladih istraživača nije pravilno prepoznat i usmeren ka stvaranju kvalifikovane populacije recenzenata. Sa idejom da doprinesemo svetskim trendovima na polju unapređenja recenziranja, predstavili smo pilot seminar na univerzitetima u Beogradu, Novom Sadu, Nišu i Kragujevcu u Srbiji u periodu od oktobra 2017. do aprila 2018. godine. Seminar u organizaciji Centra za promociju nauke obuhvatio je četiri predavanja koja su se bavila: (i) osnovnim aspektima procesa recenziranja, (ii) etičkim pitanjima, (iii) društvenim značajem i (iv) priznavanjem doprinosa recenzenata. Od ukupnog broja polaznika koji je iznosio 275, većina (84 %) se sastojala od doktoranada i mladih istraživača. Nakon seminara, učesnici su zamoljeni da izvrše test recenziju i popune anketu o kvalitetu seminara. Izveštaji o recenziji primljeni su od 82 osobe (od 275 polaznika). Od tri greške koje su namerno unete u tekst, sve greške primetilo je manje od 10 % ispitanika. Analiza ankete (160 odgovora) pokazala je da je 92% ispitanika izjavilo da su stekli nova znanja tokom seminara i ohrabrilo nastavak takve edukacije. Seminar je ponovljen 2019. g. na inicijativu Narodne biblioteke Srbije povodom globalnog događaja "Nedelja recenziranja 2019" i od anketiranih 105 novih polaznika, 94 % je iznelo stav o korisnosti obrazovanja o recenziranju i podržalo ideju o jačanju akademskih kompetencija za recenziranje kroz uvođenje obaveznog predmeta tokom doktorskih studija u Srbiji. Nadamo se da će se predstavnici akademske zajednice iz različitih naučnih disciplina pridružiti ovoj inicijativi i doprineti formiranju kurseva o recenziranju koji odgovaraju specifičnim potrebama svake naučne oblasti.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Is there a need for systematic education on peer-reviewing in Serbia?
T1  - Da li postoji potreba za sistematskom edukacijom o recenziranju u Srbiji?
VL  - 73
IS  - 5
SP  - 275
EP  - 279
DO  - 10.2298/HEMIND191020029D
ER  - 

@article{
author = "Drvenica, Ivana and Dekanski, Aleksandar and Budjevac, Nevena and Umeljic, Ivan and Nedić, Olgica",
year = "2019",
abstract = "Velika brzina stvaranja naučnih informacija u poslednjih nekoliko godina nameće potrebu za povećanjem broja stručnih recenzenata, posebno što recenziranje dobija novu dimenziju važnosti i u svetlu rastućeg broja prepoznatih plagijata i izmisljenih rezultata. Potencijalno rešenje potrebe za povećanjem broja recenzenata je sistematska edukacija doktoranada i mladih istraživača, posebno u zemljama razvoju. Prema podacima sa četiri državna univerziteta u Srbiji (u Beogradu, Novom Sadu, Nišu i Kragujevcu) i jednog privatnog univerziteta (Univerzitet Singidunum), postoji niz doktorskih kurseva koji studente obrazuju o načinu sprovođenja istraživanja, nekoliko o tome kako da pišu naučne publikacije, ali samo jedan od njih skromno osposobljava studente za recenziranje naučnih radova. Dakle, U Srbiji ogromni potencijal doktoranada i mladih istraživača nije pravilno prepoznat i usmeren ka stvaranju kvalifikovane populacije recenzenata. Sa idejom da doprinesemo svetskim trendovima na polju unapređenja recenziranja, predstavili smo pilot seminar na univerzitetima u Beogradu, Novom Sadu, Nišu i Kragujevcu u Srbiji u periodu od oktobra 2017. do aprila 2018. godine. Seminar u organizaciji Centra za promociju nauke obuhvatio je četiri predavanja koja su se bavila: (i) osnovnim aspektima procesa recenziranja, (ii) etičkim pitanjima, (iii) društvenim značajem i (iv) priznavanjem doprinosa recenzenata. Od ukupnog broja polaznika koji je iznosio 275, većina (84 %) se sastojala od doktoranada i mladih istraživača. Nakon seminara, učesnici su zamoljeni da izvrše test recenziju i popune anketu o kvalitetu seminara. Izveštaji o recenziji primljeni su od 82 osobe (od 275 polaznika). Od tri greške koje su namerno unete u tekst, sve greške primetilo je manje od 10 % ispitanika. Analiza ankete (160 odgovora) pokazala je da je 92% ispitanika izjavilo da su stekli nova znanja tokom seminara i ohrabrilo nastavak takve edukacije. Seminar je ponovljen 2019. g. na inicijativu Narodne biblioteke Srbije povodom globalnog događaja "Nedelja recenziranja 2019" i od anketiranih 105 novih polaznika, 94 % je iznelo stav o korisnosti obrazovanja o recenziranju i podržalo ideju o jačanju akademskih kompetencija za recenziranje kroz uvođenje obaveznog predmeta tokom doktorskih studija u Srbiji. Nadamo se da će se predstavnici akademske zajednice iz različitih naučnih disciplina pridružiti ovoj inicijativi i doprineti formiranju kurseva o recenziranju koji odgovaraju specifičnim potrebama svake naučne oblasti.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Is there a need for systematic education on peer-reviewing in Serbia?, Da li postoji potreba za sistematskom edukacijom o recenziranju u Srbiji?",
volume = "73",
number = "5",
pages = "275-279",
doi = "10.2298/HEMIND191020029D"
}

Drvenica, I., Dekanski, A., Budjevac, N., Umeljic, I.,& Nedić, O.. (2019). Is there a need for systematic education on peer-reviewing in Serbia?. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 73(5), 275-279.
https://doi.org/10.2298/HEMIND191020029D

Drvenica I, Dekanski A, Budjevac N, Umeljic I, Nedić O. Is there a need for systematic education on peer-reviewing in Serbia?. in Hemijska industrija. 2019;73(5):275-279.
doi:10.2298/HEMIND191020029D .

Drvenica, Ivana, Dekanski, Aleksandar, Budjevac, Nevena, Umeljic, Ivan, Nedić, Olgica, "Is there a need for systematic education on peer-reviewing in Serbia?" in Hemijska industrija, 73, no. 5 (2019):275-279,
https://doi.org/10.2298/HEMIND191020029D . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Bravo, Giangiacomo
AU  - Vejmelka, Lucija
AU  - Dekanski, Aleksandar
AU  - Nedić, Olgica
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3001
AB  - The aim of this study was to investigate the opinion of authors on the overall quality and
effectiveness of reviewers’ contributions to reviewed papers. We employed an on-line survey of thirteen journals which publish articles in the field of life, social or technological sciences. Responses received from 193 authors were analysed using a mixed-effects model in order to determine factors deemed the most important in the authors’ evaluation of the reviewers. Qualitative content analysis of the responses to open questions was performed as well. The mixed-effects model revealed that the authors’ assessment of the competence of referees strongly depended on the final editorial decision and that the speed of the review process was influential as well. In Ordinary Least Squares (OLS) analysis on seven questions detailing authors’ opinions, perception of reviewspeed remained a significant predictor of the assessment. In addition, both the perceived competence and helpfulness of the reviewers significantly and positively affected the authors’ evaluation. New models were used to re-check the value of these two factors and it was confirmed that the assessment of the competence of reviewers strongly depended on the final editorial decision.
PB  - MDPI
T2  - Publications
T1  - Peer Review of Reviewers: The Author’s Perspective
VL  - 7
IS  - 1
SP  - 1
DO  - 10.3390/publications7010001
ER  - 

@article{
author = "Drvenica, Ivana and Bravo, Giangiacomo and Vejmelka, Lucija and Dekanski, Aleksandar and Nedić, Olgica",
year = "2018",
abstract = "The aim of this study was to investigate the opinion of authors on the overall quality and
effectiveness of reviewers’ contributions to reviewed papers. We employed an on-line survey of thirteen journals which publish articles in the field of life, social or technological sciences. Responses received from 193 authors were analysed using a mixed-effects model in order to determine factors deemed the most important in the authors’ evaluation of the reviewers. Qualitative content analysis of the responses to open questions was performed as well. The mixed-effects model revealed that the authors’ assessment of the competence of referees strongly depended on the final editorial decision and that the speed of the review process was influential as well. In Ordinary Least Squares (OLS) analysis on seven questions detailing authors’ opinions, perception of reviewspeed remained a significant predictor of the assessment. In addition, both the perceived competence and helpfulness of the reviewers significantly and positively affected the authors’ evaluation. New models were used to re-check the value of these two factors and it was confirmed that the assessment of the competence of reviewers strongly depended on the final editorial decision.",
publisher = "MDPI",
journal = "Publications",
title = "Peer Review of Reviewers: The Author’s Perspective",
volume = "7",
number = "1",
pages = "1",
doi = "10.3390/publications7010001"
}

Drvenica, I., Bravo, G., Vejmelka, L., Dekanski, A.,& Nedić, O.. (2018). Peer Review of Reviewers: The Author’s Perspective. in Publications
MDPI., 7(1), 1.
https://doi.org/10.3390/publications7010001

Drvenica I, Bravo G, Vejmelka L, Dekanski A, Nedić O. Peer Review of Reviewers: The Author’s Perspective. in Publications. 2018;7(1):1.
doi:10.3390/publications7010001 .

Drvenica, Ivana, Bravo, Giangiacomo, Vejmelka, Lucija, Dekanski, Aleksandar, Nedić, Olgica, "Peer Review of Reviewers: The Author’s Perspective" in Publications, 7, no. 1 (2018):1,
https://doi.org/10.3390/publications7010001 . .

TY  - JOUR
AU  - Nedić, Olgica
AU  - Drvenica, Ivana
AU  - Ausloos, M.
AU  - Dekanski, Aleksandar
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2394
AB  - The purpose of this paper is to introduce a model for measuring the efficiency in managing peer-review of scientific manuscripts by editors. The approach employed is based on the assumption that the editorial aim is to manage publication with high efficiency, employing the least amount of editorial resources. Efficiency is defined in this research as a measure based on 7 variables. An on-line survey was constructed and editors of journals originating from Serbia regularly publishing articles in the field of chemistry were invited to participate. An evaluation of the model is given based on responses from 24 journals and 50 editors. With this investigation we aimed to contribute to our understanding of the peer-review process and, possibly, offer a tool to improve the “efficiency” in journal editing. The proposed protocol may be adapted by other journals in order to assess the managing potential of editors.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Efficiency in managing peer-review of scientific manuscripts – Editors’ perspective
VL  - 83
IS  - 12
SP  - 1391
EP  - 1405
DO  - 10.2298/JSC180531066N
ER  - 

@article{
author = "Nedić, Olgica and Drvenica, Ivana and Ausloos, M. and Dekanski, Aleksandar",
year = "2018",
abstract = "The purpose of this paper is to introduce a model for measuring the efficiency in managing peer-review of scientific manuscripts by editors. The approach employed is based on the assumption that the editorial aim is to manage publication with high efficiency, employing the least amount of editorial resources. Efficiency is defined in this research as a measure based on 7 variables. An on-line survey was constructed and editors of journals originating from Serbia regularly publishing articles in the field of chemistry were invited to participate. An evaluation of the model is given based on responses from 24 journals and 50 editors. With this investigation we aimed to contribute to our understanding of the peer-review process and, possibly, offer a tool to improve the “efficiency” in journal editing. The proposed protocol may be adapted by other journals in order to assess the managing potential of editors.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Efficiency in managing peer-review of scientific manuscripts – Editors’ perspective",
volume = "83",
number = "12",
pages = "1391-1405",
doi = "10.2298/JSC180531066N"
}

Nedić, O., Drvenica, I., Ausloos, M.,& Dekanski, A.. (2018). Efficiency in managing peer-review of scientific manuscripts – Editors’ perspective. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 83(12), 1391-1405.
https://doi.org/10.2298/JSC180531066N

Nedić O, Drvenica I, Ausloos M, Dekanski A. Efficiency in managing peer-review of scientific manuscripts – Editors’ perspective. in Journal of the Serbian Chemical Society. 2018;83(12):1391-1405.
doi:10.2298/JSC180531066N .

Nedić, Olgica, Drvenica, Ivana, Ausloos, M., Dekanski, Aleksandar, "Efficiency in managing peer-review of scientific manuscripts – Editors’ perspective" in Journal of the Serbian Chemical Society, 83, no. 12 (2018):1391-1405,
https://doi.org/10.2298/JSC180531066N . .