TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan M.
AU  - Krstić, Gordana B.
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7540
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes’ chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
VL  - 12
IS  - 24
SP  - 4181
DO  - 10.3390/plants12244181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan M. and Krstić, Gordana B.",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes’ chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
volume = "12",
number = "24",
pages = "4181",
doi = "10.3390/plants12244181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S. M.,& Krstić, G. B.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević SM, Krstić GB. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan M., Krstić, Gordana B., "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - CONF
AU  - Jadranin, Milka
AU  - Krstić, Gordana
AU  - Savić, Danica
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Lupšić, Ema
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7270
AB  - Euphorbia cyparissias L. (cypress spurge) is one of the many species of the genus Euphorbia. Like other plant species of the genus, E. cyparissias is a source of biologically active compounds [1, 2]. The aim of this research was to profile the chloroform-methanol latex extract and detect the type of secondary metabolites of Serbian E. cyparissias, collected in Deliblato Sand 
(Serbia) in May 2022, using liquid chromatography – electrospray ionisation mass spectrometry. Based on the obtained results, terpene derivatives have been found as the most abundant in the latex extract, mainly diterpenes (jatrophanes and ingenanes) and triterpenes. Also, the anticancer activ ity of the latex extract on the cell lines NCI-H460, NCI-H460/R, U87, U87/
TxR and MRC-5, as well as the ability to inhibit P-gp, were examined. Latex extract exhibited anticancer activity against all tested cell lines, and also 
proved to be a strong P-gp inhibitor. The obtained results are in accordance with the literature data because jatrophanes are well-known strong P-gp 
inhibitors [3]. These preliminary results indicate that this plant material is a rich source of biologically active compounds and detailed phytochemical research of E. cyparissias is further planned.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry
SP  - 184
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_cer_7270
ER  - 

@conference{
author = "Jadranin, Milka and Krstić, Gordana and Savić, Danica and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Pešić, Milica and Podolski-Renić, Ana and Lupšić, Ema and Milosavljević, Slobodan",
year = "2023",
abstract = "Euphorbia cyparissias L. (cypress spurge) is one of the many species of the genus Euphorbia. Like other plant species of the genus, E. cyparissias is a source of biologically active compounds [1, 2]. The aim of this research was to profile the chloroform-methanol latex extract and detect the type of secondary metabolites of Serbian E. cyparissias, collected in Deliblato Sand 
(Serbia) in May 2022, using liquid chromatography – electrospray ionisation mass spectrometry. Based on the obtained results, terpene derivatives have been found as the most abundant in the latex extract, mainly diterpenes (jatrophanes and ingenanes) and triterpenes. Also, the anticancer activ ity of the latex extract on the cell lines NCI-H460, NCI-H460/R, U87, U87/
TxR and MRC-5, as well as the ability to inhibit P-gp, were examined. Latex extract exhibited anticancer activity against all tested cell lines, and also 
proved to be a strong P-gp inhibitor. The obtained results are in accordance with the literature data because jatrophanes are well-known strong P-gp 
inhibitors [3]. These preliminary results indicate that this plant material is a rich source of biologically active compounds and detailed phytochemical research of E. cyparissias is further planned.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry",
pages = "184-185",
url = "https://hdl.handle.net/21.15107/rcub_cer_7270"
}

Jadranin, M., Krstić, G., Savić, D., Novaković, M., Lekić, S., Tešević, V., Pešić, M., Podolski-Renić, A., Lupšić, E.,& Milosavljević, S.. (2023). Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 184-185.
https://hdl.handle.net/21.15107/rcub_cer_7270

Jadranin M, Krstić G, Savić D, Novaković M, Lekić S, Tešević V, Pešić M, Podolski-Renić A, Lupšić E, Milosavljević S. Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:184-185.
https://hdl.handle.net/21.15107/rcub_cer_7270 .

Jadranin, Milka, Krstić, Gordana, Savić, Danica, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Pešić, Milica, Podolski-Renić, Ana, Lupšić, Ema, Milosavljević, Slobodan, "Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):184-185,
https://hdl.handle.net/21.15107/rcub_cer_7270 .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7268
AB  - The genus Euphorbia represents one of the largest and most diverse plant genera. Plant species of this genera are a rich source of biologically active compounds. Our previous investigation of E. palustris L. revealed an excellent anti-melanoma activity of 3β-benzoyloxy-13α-dodecanoyloxy-ingenol (1), and 3β,13α,17-tribenzoyloxy-ingenol (2), significantly better than ingenol-mebutate (Picato®), a commercial anti-melanoma agent approved by the U.S. Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the topical treatment of actinic keratosis [1]. Hence, in this research, using liquid chromatography–electrospray ionization mass spectrometry, chloroform-methanolic extracts of two samples of E. palustris collected at different localities in Serbia were analysed in order to confirm the presence of 1.Based on the tentative analysis of the LC-HRESI-MS and the previous examination of this plant species, ingenanes 1 and 2 were confirmed in both extracts examined. Additionally, both extracts contained several metabolites that structurally corresponds to ingenol, with a decanoyl group in their structure. Based on these preliminary results, our goal in further investigation is to isolate other ingenane molecules and test their anti-melanoma activity.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract
SP  - 43
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_cer_7268
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Savić, Danica and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "The genus Euphorbia represents one of the largest and most diverse plant genera. Plant species of this genera are a rich source of biologically active compounds. Our previous investigation of E. palustris L. revealed an excellent anti-melanoma activity of 3β-benzoyloxy-13α-dodecanoyloxy-ingenol (1), and 3β,13α,17-tribenzoyloxy-ingenol (2), significantly better than ingenol-mebutate (Picato®), a commercial anti-melanoma agent approved by the U.S. Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the topical treatment of actinic keratosis [1]. Hence, in this research, using liquid chromatography–electrospray ionization mass spectrometry, chloroform-methanolic extracts of two samples of E. palustris collected at different localities in Serbia were analysed in order to confirm the presence of 1.Based on the tentative analysis of the LC-HRESI-MS and the previous examination of this plant species, ingenanes 1 and 2 were confirmed in both extracts examined. Additionally, both extracts contained several metabolites that structurally corresponds to ingenol, with a decanoyl group in their structure. Based on these preliminary results, our goal in further investigation is to isolate other ingenane molecules and test their anti-melanoma activity.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract",
pages = "43-44",
url = "https://hdl.handle.net/21.15107/rcub_cer_7268"
}

Krstić, G., Jadranin, M., Savić, D., Novaković, M., Lekić, S., Tešević, V.,& Milosavljević, S.. (2023). LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 43-44.
https://hdl.handle.net/21.15107/rcub_cer_7268

Krstić G, Jadranin M, Savić D, Novaković M, Lekić S, Tešević V, Milosavljević S. LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:43-44.
https://hdl.handle.net/21.15107/rcub_cer_7268 .

Krstić, Gordana, Jadranin, Milka, Savić, Danica, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Milosavljević, Slobodan, "LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):43-44,
https://hdl.handle.net/21.15107/rcub_cer_7268 .

TY  - CONF
AU  - Novaković, Miroslav
AU  - Jadranin, Milka
AU  - Lekić, Stefan
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
AB  - Bisbibenzyls represent chemical class of compounds containing two bibenzyl units mutually connected by an oxygen atom and/or directly by the “C-C” bonds. They are chemical markers of liverworts, the ancestors of higher vascular plants. Marchantin A from Marchantia polymorpha was the first discovered bisbibenzyl. More than 120 chemically characterized bisbibenzyls 
were reported up to 2020 with numerous biological activities [1]. The first report on bisbibenzyls in higher (vascular) plants was published in 2007, when 
Kosenkova et al. (2007) isolated riccardin C in Primula veris subsp. macrocalyx from Mt. Altay in Russia as the dominant compound in the extract of the whole plant [2]. In the repeated study of the same species, Kosenkova et al. (2009) confirmed their findings discovering an additional bisbibenzyl constituent, perrottetin E [3].
In this investigation from the CH2Cl2/CH3OH (1:1) extract of the air-dried, powdered roots nine isbibenzyls, five new, have been isolated from P. veris subsp. columnae and P. acaulis using dry column flash chromatography followed by semipreparative HPLC chromatography and identified on the basis of 1D and 2D NMR, IR, UV and HRESIMS data. This investigation widens the knowledge about the presence of bisbibenzyls in vascular plants since only two bisbibenzyls have been previously found in vascular plants, in Primula veris subsp. macrocalyx.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Bisbibenzyls in Primula species
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_cer_7269
ER  - 

@conference{
author = "Novaković, Miroslav and Jadranin, Milka and Lekić, Stefan and Savić, Danica and Krstić, Gordana and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "Bisbibenzyls represent chemical class of compounds containing two bibenzyl units mutually connected by an oxygen atom and/or directly by the “C-C” bonds. They are chemical markers of liverworts, the ancestors of higher vascular plants. Marchantin A from Marchantia polymorpha was the first discovered bisbibenzyl. More than 120 chemically characterized bisbibenzyls 
were reported up to 2020 with numerous biological activities [1]. The first report on bisbibenzyls in higher (vascular) plants was published in 2007, when 
Kosenkova et al. (2007) isolated riccardin C in Primula veris subsp. macrocalyx from Mt. Altay in Russia as the dominant compound in the extract of the whole plant [2]. In the repeated study of the same species, Kosenkova et al. (2009) confirmed their findings discovering an additional bisbibenzyl constituent, perrottetin E [3].
In this investigation from the CH2Cl2/CH3OH (1:1) extract of the air-dried, powdered roots nine isbibenzyls, five new, have been isolated from P. veris subsp. columnae and P. acaulis using dry column flash chromatography followed by semipreparative HPLC chromatography and identified on the basis of 1D and 2D NMR, IR, UV and HRESIMS data. This investigation widens the knowledge about the presence of bisbibenzyls in vascular plants since only two bisbibenzyls have been previously found in vascular plants, in Primula veris subsp. macrocalyx.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Bisbibenzyls in Primula species",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_cer_7269"
}

Novaković, M., Jadranin, M., Lekić, S., Savić, D., Krstić, G., Tešević, V.,& Milosavljević, S.. (2023). Bisbibenzyls in Primula species. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 47-48.
https://hdl.handle.net/21.15107/rcub_cer_7269

Novaković M, Jadranin M, Lekić S, Savić D, Krstić G, Tešević V, Milosavljević S. Bisbibenzyls in Primula species. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:47-48.
https://hdl.handle.net/21.15107/rcub_cer_7269 .

Novaković, Miroslav, Jadranin, Milka, Lekić, Stefan, Savić, Danica, Krstić, Gordana, Tešević, Vele, Milosavljević, Slobodan, "Bisbibenzyls in Primula species" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):47-48,
https://hdl.handle.net/21.15107/rcub_cer_7269 .

TY  - CONF
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7272
AB  - The diversity of plant species from the genus Euphorbia and wide variety of secondary metabolites they produce contributed to their successful use in the traditional medicine all over the world. Therapeutic applications have been attributed to different compounds such as diterpenes and triterpenes. Previous investigations revealed that Euphorbia diterpenes possess a variety of biological activities such as anti-inflammatory, antitumor and antiviral. Some of them showed that tigliane diterpenes, beside other potential pharmaceutical activities, inhibited wild-type HIV-1 and HIV-2 strains as well as drug resistant strains of HIV 1 and that they exhibited low cytotoxicity. Two tigliane diterpenes were 
isolated from the latex of E. lucida, using classical and instrumental chromatographic techniques. ESI-HRMS spectra showed that both isolated compounds possess the same molecular formula (C31H38O6). 1D NMR spectra revealed benzoate and isobutanoate ester groups in both molecules, Using HMBC spectra their position was determined: C-13 for isobutanoate and C-20 for benzoate. Exact configurations were discovered by NOE correlations. It was concluded that these two molecules differ in the way of the binding the five-membered and seven-membered rings, i.e., that these two molecules are C-4-epimers. Compound 1 (20-benzoyloxy-13α-isobutanoyloxy-4,12-dideoxyphorbol) was already described in the literature as a metabolite of E. pannonica [1], while compound 2 (20-benzoyloxy-13α-isobutanoyloxy-4-epi-4,12-dideoxyphorbol) represents a new tigliane derivative.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Tigliane diterpenes isolated from the latex of Euphorbia lucida
SP  - 239
EP  - 239
UR  - https://hdl.handle.net/21.15107/rcub_cer_7272
ER  - 

@conference{
author = "Savić, Danica and Krstić, Gordana and Jadranin, Milka and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "The diversity of plant species from the genus Euphorbia and wide variety of secondary metabolites they produce contributed to their successful use in the traditional medicine all over the world. Therapeutic applications have been attributed to different compounds such as diterpenes and triterpenes. Previous investigations revealed that Euphorbia diterpenes possess a variety of biological activities such as anti-inflammatory, antitumor and antiviral. Some of them showed that tigliane diterpenes, beside other potential pharmaceutical activities, inhibited wild-type HIV-1 and HIV-2 strains as well as drug resistant strains of HIV 1 and that they exhibited low cytotoxicity. Two tigliane diterpenes were 
isolated from the latex of E. lucida, using classical and instrumental chromatographic techniques. ESI-HRMS spectra showed that both isolated compounds possess the same molecular formula (C31H38O6). 1D NMR spectra revealed benzoate and isobutanoate ester groups in both molecules, Using HMBC spectra their position was determined: C-13 for isobutanoate and C-20 for benzoate. Exact configurations were discovered by NOE correlations. It was concluded that these two molecules differ in the way of the binding the five-membered and seven-membered rings, i.e., that these two molecules are C-4-epimers. Compound 1 (20-benzoyloxy-13α-isobutanoyloxy-4,12-dideoxyphorbol) was already described in the literature as a metabolite of E. pannonica [1], while compound 2 (20-benzoyloxy-13α-isobutanoyloxy-4-epi-4,12-dideoxyphorbol) represents a new tigliane derivative.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Tigliane diterpenes isolated from the latex of Euphorbia lucida",
pages = "239-239",
url = "https://hdl.handle.net/21.15107/rcub_cer_7272"
}

Savić, D., Krstić, G., Jadranin, M., Novaković, M., Lekić, S., Tešević, V.,& Milosavljević, S.. (2023). Tigliane diterpenes isolated from the latex of Euphorbia lucida. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 239-239.
https://hdl.handle.net/21.15107/rcub_cer_7272

Savić D, Krstić G, Jadranin M, Novaković M, Lekić S, Tešević V, Milosavljević S. Tigliane diterpenes isolated from the latex of Euphorbia lucida. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:239-239.
https://hdl.handle.net/21.15107/rcub_cer_7272 .

Savić, Danica, Krstić, Gordana, Jadranin, Milka, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Milosavljević, Slobodan, "Tigliane diterpenes isolated from the latex of Euphorbia lucida" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):239-239,
https://hdl.handle.net/21.15107/rcub_cer_7272 .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Todorović, Nina
AU  - Jadranin, Milka
AU  - Đorđević, Iris
AU  - Milosavljević, Slobodan M.
AU  - Mandić, Boris
AU  - Tešević, Vele
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7191
AB  - A new auronolignan, named cotinignan B, was isolated from Cotinus coggygria Scop. Structure elucidation was performed on the basis of 1H, 13C NMR, COSY, NOESY, HSQC, and HMBC experiments, supported with HR-ESI-MS, IR and UV. Additional J-HMBC NMR experiment was essential to resolve the configuration of the trisubstituted double bond. This compound represents the secondly discovered natural compound belonging to the rare auronolignan type.
PB  - Springer
T2  - Chemistry of Natural Compounds
T1  - A New Auronolignan from the Cotinus coggygria Heartwood
VL  - 59
IS  - 3
SP  - 428
EP  - 430
DO  - 10.1007/s10600-023-04016-5
ER  - 

@article{
author = "Novaković, Miroslav and Todorović, Nina and Jadranin, Milka and Đorđević, Iris and Milosavljević, Slobodan M. and Mandić, Boris and Tešević, Vele",
year = "2023",
abstract = "A new auronolignan, named cotinignan B, was isolated from Cotinus coggygria Scop. Structure elucidation was performed on the basis of 1H, 13C NMR, COSY, NOESY, HSQC, and HMBC experiments, supported with HR-ESI-MS, IR and UV. Additional J-HMBC NMR experiment was essential to resolve the configuration of the trisubstituted double bond. This compound represents the secondly discovered natural compound belonging to the rare auronolignan type.",
publisher = "Springer",
journal = "Chemistry of Natural Compounds",
title = "A New Auronolignan from the Cotinus coggygria Heartwood",
volume = "59",
number = "3",
pages = "428-430",
doi = "10.1007/s10600-023-04016-5"
}

Novaković, M., Todorović, N., Jadranin, M., Đorđević, I., Milosavljević, S. M., Mandić, B.,& Tešević, V.. (2023). A New Auronolignan from the Cotinus coggygria Heartwood. in Chemistry of Natural Compounds
Springer., 59(3), 428-430.
https://doi.org/10.1007/s10600-023-04016-5

Novaković M, Todorović N, Jadranin M, Đorđević I, Milosavljević SM, Mandić B, Tešević V. A New Auronolignan from the Cotinus coggygria Heartwood. in Chemistry of Natural Compounds. 2023;59(3):428-430.
doi:10.1007/s10600-023-04016-5 .

Novaković, Miroslav, Todorović, Nina, Jadranin, Milka, Đorđević, Iris, Milosavljević, Slobodan M., Mandić, Boris, Tešević, Vele, "A New Auronolignan from the Cotinus coggygria Heartwood" in Chemistry of Natural Compounds, 59, no. 3 (2023):428-430,
https://doi.org/10.1007/s10600-023-04016-5 . .

TY  - JOUR
AU  - Janjić, Goran
AU  - Marinović, Sanja
AU  - Jadranin, Milka
AU  - Ajduković, Marija
AU  - Đorđević, Ivana
AU  - Petkovic-Benazzouz, Marija
AU  - Milutinović Nikolić, Aleksandra
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6693
AB  - The catalytic degradation of hazardous organic contaminants in industrial wastewater is a promising technology. Reactions of tartrazine, the synthetic yellow azo dye, with Oxone® in the presence of catalyst in strong acidic condition (pH 2), were detected by using UV–Vis spectroscopy. In order to extend the applicability profile of Co-supported Al-pillared montmorillonite catalyst an investigation of Oxone® induced reactions were performed in extreme acidic environment. The products of the reactions were identified by liquid chromatography–mass spectrometry (LC-MS). Along with the catalytic decomposition of tartrazine induced by radical attack (confirmed as unique reaction path under neutral and alkaline conditions), the formation of tartrazine derivatives by reaction of nucleophilic addition was also detected. The presence of derivatives under acidic conditions slowed down the hydrolysis of tartrazine diazo bond in comparison to the reactions in neutral environment. Nevertheless, the reaction in acidic conditions (pH 2) is faster than the one conducted in alkaline conditions (pH 11). Theoretical calculations were used to complete and clarify the mechanisms of tartrazine derivatization and degradation, as well as to predict the UV–Vis spectra of compounds which could serve as predictors of certain reaction phases. ECOSAR program, used to estimate toxicological profile of compounds to aquatic animals, indicated an increase in the harmfulness of the compounds identified by LC-MS as degradation products from the reaction conducted for 240min. It could be concluded that an intensification of the process parameters (higher concentration of Oxone®, higher catalyst loading, increased reaction time, etc.) is needed in order to obtain only biodegradable products.
PB  - Elsevier
T2  - Environmental Pollution
T1  - Degradation of tartrazine by Oxone® in the presence of cobalt based catalyst supported on pillared montmorillonite - Efficient technology even in extreme conditions
VL  - 331
SP  - 121863
DO  - 10.1016/j.envpol.2023.121863
ER  - 

@article{
author = "Janjić, Goran and Marinović, Sanja and Jadranin, Milka and Ajduković, Marija and Đorđević, Ivana and Petkovic-Benazzouz, Marija and Milutinović Nikolić, Aleksandra",
year = "2023",
abstract = "The catalytic degradation of hazardous organic contaminants in industrial wastewater is a promising technology. Reactions of tartrazine, the synthetic yellow azo dye, with Oxone® in the presence of catalyst in strong acidic condition (pH 2), were detected by using UV–Vis spectroscopy. In order to extend the applicability profile of Co-supported Al-pillared montmorillonite catalyst an investigation of Oxone® induced reactions were performed in extreme acidic environment. The products of the reactions were identified by liquid chromatography–mass spectrometry (LC-MS). Along with the catalytic decomposition of tartrazine induced by radical attack (confirmed as unique reaction path under neutral and alkaline conditions), the formation of tartrazine derivatives by reaction of nucleophilic addition was also detected. The presence of derivatives under acidic conditions slowed down the hydrolysis of tartrazine diazo bond in comparison to the reactions in neutral environment. Nevertheless, the reaction in acidic conditions (pH 2) is faster than the one conducted in alkaline conditions (pH 11). Theoretical calculations were used to complete and clarify the mechanisms of tartrazine derivatization and degradation, as well as to predict the UV–Vis spectra of compounds which could serve as predictors of certain reaction phases. ECOSAR program, used to estimate toxicological profile of compounds to aquatic animals, indicated an increase in the harmfulness of the compounds identified by LC-MS as degradation products from the reaction conducted for 240min. It could be concluded that an intensification of the process parameters (higher concentration of Oxone®, higher catalyst loading, increased reaction time, etc.) is needed in order to obtain only biodegradable products.",
publisher = "Elsevier",
journal = "Environmental Pollution",
title = "Degradation of tartrazine by Oxone® in the presence of cobalt based catalyst supported on pillared montmorillonite - Efficient technology even in extreme conditions",
volume = "331",
pages = "121863",
doi = "10.1016/j.envpol.2023.121863"
}

Janjić, G., Marinović, S., Jadranin, M., Ajduković, M., Đorđević, I., Petkovic-Benazzouz, M.,& Milutinović Nikolić, A.. (2023). Degradation of tartrazine by Oxone® in the presence of cobalt based catalyst supported on pillared montmorillonite - Efficient technology even in extreme conditions. in Environmental Pollution
Elsevier., 331, 121863.
https://doi.org/10.1016/j.envpol.2023.121863

Janjić G, Marinović S, Jadranin M, Ajduković M, Đorđević I, Petkovic-Benazzouz M, Milutinović Nikolić A. Degradation of tartrazine by Oxone® in the presence of cobalt based catalyst supported on pillared montmorillonite - Efficient technology even in extreme conditions. in Environmental Pollution. 2023;331:121863.
doi:10.1016/j.envpol.2023.121863 .

Janjić, Goran, Marinović, Sanja, Jadranin, Milka, Ajduković, Marija, Đorđević, Ivana, Petkovic-Benazzouz, Marija, Milutinović Nikolić, Aleksandra, "Degradation of tartrazine by Oxone® in the presence of cobalt based catalyst supported on pillared montmorillonite - Efficient technology even in extreme conditions" in Environmental Pollution, 331 (2023):121863,
https://doi.org/10.1016/j.envpol.2023.121863 . .

TY  - JOUR
AU  - Zornić, Sanja
AU  - Simović Marković, Bojana
AU  - Franich, Andjela A.
AU  - Janjić, Goran
AU  - Jadranin, Milka
AU  - Avdalović, Jelena
AU  - Rajković, Snežana
AU  - Živković, Marija D.
AU  - Arsenijević, Nebojša N.
AU  - Radosavljević, Gordana D.
AU  - Pantić, Jelena
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7213
AB  - Platinum-based drugs are widely recognized efficient anti-tumor agents, but faced with multiple undesirable effects. Here, four dinuclear platinum(II) complexes, [{Pt(1,2-pn)Cl}2(μ-pydz)]Cl2 (C1), [{Pt(ibn)Cl}2(μ-pydz)]Cl2 (C2), [{Pt(1,3-pn)Cl}2(μ-pydz)]Cl2 (C3) and [{Pt(1,3-pnd)Cl}2(μ-pydz)]Cl2 (C4), were designed (pydz is pyridazine, 1,2-pn is ( ±)-1,2-propylenediamine, ibn is 1,2-diamino-2-methylpropane, 1,3-pn is 1,3-propylenediamine, and 1,3-pnd is 1,3-pentanediamine). Interactions and binding ability of C1–C4 complexes with calf thymus DNA (CT-DNA) has been monitored by viscosity measurements, UV–Vis, fluorescence emission spectroscopy and molecular docking. Binding affinities of C1–C4 complexes to the bovine serum albumin (BSA) has been monitored by fluorescence emission spectroscopy. The tested complexes exhibit variable cytotoxicity toward different mouse and human tumor cell lines. C2 shows the most potent cytotoxicity, especially against mouse (4T1) and human (MDA-MD468) breast cancer cells in the dose- and time-dependent manner. C2 induces 4T1 and MDA-MD468 cells apoptosis, further documented by the accumulation of cells at sub-G1 phase of cell cycle and increase of executive caspase 3 and caspase 9 levels in 4T1 cells. C2 exhibits anti-proliferative effect through the reduction of cyclin D3 and cyclin E expression and elevation of inhibitor p27 level. Also, C2 downregulates c-Myc and phosphorylated AKT, oncogenes involved in the control of tumor cell proliferation and death. In order to measure the amount of platinum(II) complexes taken up by the cells, the cellular platinum content were quantified. However, C2 failed to inhibit mouse breast cancer growth in vivo. Chemical modifications of tested platinum(II) complexes might be a valuable approach for the improvement of their anti-tumor activity, especially effects in vivo.
PB  - Springer
T2  - Journal of Biological Inorganic Chemistry
T1  - Characterization, modes of interactions with DNA/BSA biomolecules and anti-tumor activity of newly synthesized dinuclear platinum(II) complexes with pyridazine bridging ligand
DO  - 10.1007/s00775-023-02030-0
ER  - 

@article{
author = "Zornić, Sanja and Simović Marković, Bojana and Franich, Andjela A. and Janjić, Goran and Jadranin, Milka and Avdalović, Jelena and Rajković, Snežana and Živković, Marija D. and Arsenijević, Nebojša N. and Radosavljević, Gordana D. and Pantić, Jelena",
year = "2023",
abstract = "Platinum-based drugs are widely recognized efficient anti-tumor agents, but faced with multiple undesirable effects. Here, four dinuclear platinum(II) complexes, [{Pt(1,2-pn)Cl}2(μ-pydz)]Cl2 (C1), [{Pt(ibn)Cl}2(μ-pydz)]Cl2 (C2), [{Pt(1,3-pn)Cl}2(μ-pydz)]Cl2 (C3) and [{Pt(1,3-pnd)Cl}2(μ-pydz)]Cl2 (C4), were designed (pydz is pyridazine, 1,2-pn is ( ±)-1,2-propylenediamine, ibn is 1,2-diamino-2-methylpropane, 1,3-pn is 1,3-propylenediamine, and 1,3-pnd is 1,3-pentanediamine). Interactions and binding ability of C1–C4 complexes with calf thymus DNA (CT-DNA) has been monitored by viscosity measurements, UV–Vis, fluorescence emission spectroscopy and molecular docking. Binding affinities of C1–C4 complexes to the bovine serum albumin (BSA) has been monitored by fluorescence emission spectroscopy. The tested complexes exhibit variable cytotoxicity toward different mouse and human tumor cell lines. C2 shows the most potent cytotoxicity, especially against mouse (4T1) and human (MDA-MD468) breast cancer cells in the dose- and time-dependent manner. C2 induces 4T1 and MDA-MD468 cells apoptosis, further documented by the accumulation of cells at sub-G1 phase of cell cycle and increase of executive caspase 3 and caspase 9 levels in 4T1 cells. C2 exhibits anti-proliferative effect through the reduction of cyclin D3 and cyclin E expression and elevation of inhibitor p27 level. Also, C2 downregulates c-Myc and phosphorylated AKT, oncogenes involved in the control of tumor cell proliferation and death. In order to measure the amount of platinum(II) complexes taken up by the cells, the cellular platinum content were quantified. However, C2 failed to inhibit mouse breast cancer growth in vivo. Chemical modifications of tested platinum(II) complexes might be a valuable approach for the improvement of their anti-tumor activity, especially effects in vivo.",
publisher = "Springer",
journal = "Journal of Biological Inorganic Chemistry",
title = "Characterization, modes of interactions with DNA/BSA biomolecules and anti-tumor activity of newly synthesized dinuclear platinum(II) complexes with pyridazine bridging ligand",
doi = "10.1007/s00775-023-02030-0"
}

Zornić, S., Simović Marković, B., Franich, A. A., Janjić, G., Jadranin, M., Avdalović, J., Rajković, S., Živković, M. D., Arsenijević, N. N., Radosavljević, G. D.,& Pantić, J.. (2023). Characterization, modes of interactions with DNA/BSA biomolecules and anti-tumor activity of newly synthesized dinuclear platinum(II) complexes with pyridazine bridging ligand. in Journal of Biological Inorganic Chemistry
Springer..
https://doi.org/10.1007/s00775-023-02030-0

Zornić S, Simović Marković B, Franich AA, Janjić G, Jadranin M, Avdalović J, Rajković S, Živković MD, Arsenijević NN, Radosavljević GD, Pantić J. Characterization, modes of interactions with DNA/BSA biomolecules and anti-tumor activity of newly synthesized dinuclear platinum(II) complexes with pyridazine bridging ligand. in Journal of Biological Inorganic Chemistry. 2023;.
doi:10.1007/s00775-023-02030-0 .

Zornić, Sanja, Simović Marković, Bojana, Franich, Andjela A., Janjić, Goran, Jadranin, Milka, Avdalović, Jelena, Rajković, Snežana, Živković, Marija D., Arsenijević, Nebojša N., Radosavljević, Gordana D., Pantić, Jelena, "Characterization, modes of interactions with DNA/BSA biomolecules and anti-tumor activity of newly synthesized dinuclear platinum(II) complexes with pyridazine bridging ligand" in Journal of Biological Inorganic Chemistry (2023),
https://doi.org/10.1007/s00775-023-02030-0 . .

TY  - JOUR
AU  - Jadranin, Milka
AU  - Avramović, Nataša
AU  - Miladinović, Zoran P.
AU  - Gavrilović, Aleksandra
AU  - Tasic, Ljubica
AU  - Tešević, Vele
AU  - Mandić, Boris
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7180
AB  - The Lipidomic profiles of serum samples from patients with bipolar disorder (BD) and healthy controls (C) were explored and compared. The sample cohort included 31 BD patients and 31 control individuals. An untargeted lipidomics study applying liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) was conducted to achieve the lipid profiles. Multivariate statistical analyses (principal component analysis and partial least squares discriminant analysis) were performed, and fifty-six differential lipids were confirmed in BD and controls. Our results pointed to alterations in lipid metabolism, including pathways of glycerophospholipids, sphingolipids, glycerolipids, and sterol lipids, in BD patient sera. This study emphasized the role of lipid pathways in BD, and comprehensive research using the LC-HRMS platform is necessary for future application in the diagnosis and improvement of BD treatments.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia
VL  - 24
IS  - 22
SP  - 16025
DO  - 10.3390/ijms242216025
ER  - 

@article{
author = "Jadranin, Milka and Avramović, Nataša and Miladinović, Zoran P. and Gavrilović, Aleksandra and Tasic, Ljubica and Tešević, Vele and Mandić, Boris",
year = "2023",
abstract = "The Lipidomic profiles of serum samples from patients with bipolar disorder (BD) and healthy controls (C) were explored and compared. The sample cohort included 31 BD patients and 31 control individuals. An untargeted lipidomics study applying liquid chromatography (LC) coupled with high-resolution mass spectrometry (HRMS) was conducted to achieve the lipid profiles. Multivariate statistical analyses (principal component analysis and partial least squares discriminant analysis) were performed, and fifty-six differential lipids were confirmed in BD and controls. Our results pointed to alterations in lipid metabolism, including pathways of glycerophospholipids, sphingolipids, glycerolipids, and sterol lipids, in BD patient sera. This study emphasized the role of lipid pathways in BD, and comprehensive research using the LC-HRMS platform is necessary for future application in the diagnosis and improvement of BD treatments.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia",
volume = "24",
number = "22",
pages = "16025",
doi = "10.3390/ijms242216025"
}

Jadranin, M., Avramović, N., Miladinović, Z. P., Gavrilović, A., Tasic, L., Tešević, V.,& Mandić, B.. (2023). Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia. in International Journal of Molecular Sciences
MDPI., 24(22), 16025.
https://doi.org/10.3390/ijms242216025

Jadranin M, Avramović N, Miladinović ZP, Gavrilović A, Tasic L, Tešević V, Mandić B. Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia. in International Journal of Molecular Sciences. 2023;24(22):16025.
doi:10.3390/ijms242216025 .

Jadranin, Milka, Avramović, Nataša, Miladinović, Zoran P., Gavrilović, Aleksandra, Tasic, Ljubica, Tešević, Vele, Mandić, Boris, "Untargeted Lipidomics Study of Bipolar Disorder Patients in Serbia" in International Journal of Molecular Sciences, 24, no. 22 (2023):16025,
https://doi.org/10.3390/ijms242216025 . .

TY  - JOUR
AU  - Nicoleti, Jorge Luis
AU  - Braga, Erik Sobrinho
AU  - Stanisic, Danijela
AU  - Jadranin, Milka
AU  - Façanha, Débora Andréa Evangelista
AU  - Barral, Thiago Doria
AU  - Hanna, Samira Abdallah
AU  - Azevedo, Vasco
AU  - Meyer, Roberto
AU  - Tasic, Ljubica
AU  - Portela, Ricardo Wagner
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7094
AB  - Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host–pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance (1H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr–Purcell–Meiboom–Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD+, and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis.
PB  - Springer Science and Business Media LLC
T2  - Applied Microbiology and Biotechnology
T1  - A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats
VL  - 107
IS  - 14
SP  - 4593
EP  - 4603
DO  - 10.1007/s00253-023-12595-0
ER  - 

@article{
author = "Nicoleti, Jorge Luis and Braga, Erik Sobrinho and Stanisic, Danijela and Jadranin, Milka and Façanha, Débora Andréa Evangelista and Barral, Thiago Doria and Hanna, Samira Abdallah and Azevedo, Vasco and Meyer, Roberto and Tasic, Ljubica and Portela, Ricardo Wagner",
year = "2023",
abstract = "Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host–pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance (1H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr–Purcell–Meiboom–Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD+, and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis.",
publisher = "Springer Science and Business Media LLC",
journal = "Applied Microbiology and Biotechnology",
title = "A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats",
volume = "107",
number = "14",
pages = "4593-4603",
doi = "10.1007/s00253-023-12595-0"
}

Nicoleti, J. L., Braga, E. S., Stanisic, D., Jadranin, M., Façanha, D. A. E., Barral, T. D., Hanna, S. A., Azevedo, V., Meyer, R., Tasic, L.,& Portela, R. W.. (2023). A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats. in Applied Microbiology and Biotechnology
Springer Science and Business Media LLC., 107(14), 4593-4603.
https://doi.org/10.1007/s00253-023-12595-0

Nicoleti JL, Braga ES, Stanisic D, Jadranin M, Façanha DAE, Barral TD, Hanna SA, Azevedo V, Meyer R, Tasic L, Portela RW. A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats. in Applied Microbiology and Biotechnology. 2023;107(14):4593-4603.
doi:10.1007/s00253-023-12595-0 .

Nicoleti, Jorge Luis, Braga, Erik Sobrinho, Stanisic, Danijela, Jadranin, Milka, Façanha, Débora Andréa Evangelista, Barral, Thiago Doria, Hanna, Samira Abdallah, Azevedo, Vasco, Meyer, Roberto, Tasic, Ljubica, Portela, Ricardo Wagner, "A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats" in Applied Microbiology and Biotechnology, 107, no. 14 (2023):4593-4603,
https://doi.org/10.1007/s00253-023-12595-0 . .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5862
AB  - In this research, from two species, E. palustris and E.  lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma  – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was  determined using normal human keratinocytes – HaCaT.
PB  - Macedonian Pharmaceutical Association
C3  - Macedonian Pharmaceutical Bulletin
T1  - Anti-melanoma effects of ingenanes isolated from Euphorbia species
VL  - 68
IS  - Suppl.2
SP  - 23
EP  - 24
DO  - 10.33320/maced.pharm.bull.2022.68.04.006
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Jovanović Stojanov, Sofija and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "In this research, from two species, E. palustris and E.  lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma  – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was  determined using normal human keratinocytes – HaCaT.",
publisher = "Macedonian Pharmaceutical Association",
journal = "Macedonian Pharmaceutical Bulletin",
title = "Anti-melanoma effects of ingenanes isolated from Euphorbia species",
volume = "68",
number = "Suppl.2",
pages = "23-24",
doi = "10.33320/maced.pharm.bull.2022.68.04.006"
}

Krstić, G., Jadranin, M., Jovanović Stojanov, S., Pešić, M., Tešević, V.,& Milosavljević, S.. (2022). Anti-melanoma effects of ingenanes isolated from Euphorbia species. in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68(Suppl.2), 23-24.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006

Krstić G, Jadranin M, Jovanović Stojanov S, Pešić M, Tešević V, Milosavljević S. Anti-melanoma effects of ingenanes isolated from Euphorbia species. in Macedonian Pharmaceutical Bulletin. 2022;68(Suppl.2):23-24.
doi:10.33320/maced.pharm.bull.2022.68.04.006 .

Krstić, Gordana, Jadranin, Milka, Jovanović Stojanov, Sofija, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, "Anti-melanoma effects of ingenanes isolated from Euphorbia species" in Macedonian Pharmaceutical Bulletin, 68, no. Suppl.2 (2022):23-24,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006 . .

TY  - CONF
AU  - Jadranin, Milka
AU  - Cvetković, Mirjana
AU  - Đorđević, Iris
AU  - Krstić, Gordana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5863
AB  - This study revealed results from reinvestigation of  sesquiterpene lactones composition in the crude and leaf  surface extracts of Amphoricarpos plants by rapid  resolution liquid chromatography (RRLC) coupled with  quadrupole time-of-flight mass spectrometry (QToF MS)  and the tentative identification of undescribed  sesquiterpene lactones is presented.
PB  - Macedonian Pharmaceutical Association
C3  - Macedonian Pharmaceutical Bulletin
T1  - New insights into sesquiterpene lactones composition of 
Western Balkan’s genus Amphoricarpos revealed by rapid 
resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
VL  - 68
IS  - Suppl.2
SP  - 71
EP  - 72
DO  - 10.33320/maced.pharm.bull.2022.68.04.030
ER  - 

@conference{
author = "Jadranin, Milka and Cvetković, Mirjana and Đorđević, Iris and Krstić, Gordana and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "This study revealed results from reinvestigation of  sesquiterpene lactones composition in the crude and leaf  surface extracts of Amphoricarpos plants by rapid  resolution liquid chromatography (RRLC) coupled with  quadrupole time-of-flight mass spectrometry (QToF MS)  and the tentative identification of undescribed  sesquiterpene lactones is presented.",
publisher = "Macedonian Pharmaceutical Association",
journal = "Macedonian Pharmaceutical Bulletin",
title = "New insights into sesquiterpene lactones composition of 
Western Balkan’s genus Amphoricarpos revealed by rapid 
resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry",
volume = "68",
number = "Suppl.2",
pages = "71-72",
doi = "10.33320/maced.pharm.bull.2022.68.04.030"
}

Jadranin, M., Cvetković, M., Đorđević, I., Krstić, G., Tešević, V.,& Milosavljević, S.. (2022). New insights into sesquiterpene lactones composition of 
Western Balkan’s genus Amphoricarpos revealed by rapid 
resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68(Suppl.2), 71-72.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.030

Jadranin M, Cvetković M, Đorđević I, Krstić G, Tešević V, Milosavljević S. New insights into sesquiterpene lactones composition of 
Western Balkan’s genus Amphoricarpos revealed by rapid 
resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. in Macedonian Pharmaceutical Bulletin. 2022;68(Suppl.2):71-72.
doi:10.33320/maced.pharm.bull.2022.68.04.030 .

Jadranin, Milka, Cvetković, Mirjana, Đorđević, Iris, Krstić, Gordana, Tešević, Vele, Milosavljević, Slobodan, "New insights into sesquiterpene lactones composition of 
Western Balkan’s genus Amphoricarpos revealed by rapid 
resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry" in Macedonian Pharmaceutical Bulletin, 68, no. Suppl.2 (2022):71-72,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.030 . .

TY  - CONF
AU  - Jadranin, Milka
AU  - Krstić, Gordana
AU  - Novaković, Miroslav
AU  - Anđelković, Boban
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5864
AB  - In this study, we analysed the chloroform extract of the latex of E. seguieriana Neck. subsp. 
seguieriana collected on Deliblato sand in flowering season by reversed-phase liquid chromatography–electrospray ionisation mass spectrometry (LC-ESI MS). From the data obtained from LC-ESI MS analysis, premyrsinane, myrsinane and lathyrane nicotinoyl esters are the dominant diterpene metabolites of the latex. In the continuation of the research, compounds will be isolated, their structures elucidated, and their biological activities examined.
PB  - Belgrade : University of Belgrade - Faculty of Agriculture
C3  - Book of Abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF), 7–9 September 2022, Belgrade, Serbia
T1  - Analysis of diterpenoids from the latex of E. seguieriana Neck. subsp. seguieriana by liquid chromatography–electrospray ionisation mass spectrometry
SP  - 86
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_cer_5864
ER  - 

@conference{
author = "Jadranin, Milka and Krstić, Gordana and Novaković, Miroslav and Anđelković, Boban and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "In this study, we analysed the chloroform extract of the latex of E. seguieriana Neck. subsp. 
seguieriana collected on Deliblato sand in flowering season by reversed-phase liquid chromatography–electrospray ionisation mass spectrometry (LC-ESI MS). From the data obtained from LC-ESI MS analysis, premyrsinane, myrsinane and lathyrane nicotinoyl esters are the dominant diterpene metabolites of the latex. In the continuation of the research, compounds will be isolated, their structures elucidated, and their biological activities examined.",
publisher = "Belgrade : University of Belgrade - Faculty of Agriculture",
journal = "Book of Abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF), 7–9 September 2022, Belgrade, Serbia",
title = "Analysis of diterpenoids from the latex of E. seguieriana Neck. subsp. seguieriana by liquid chromatography–electrospray ionisation mass spectrometry",
pages = "86-86",
url = "https://hdl.handle.net/21.15107/rcub_cer_5864"
}

Jadranin, M., Krstić, G., Novaković, M., Anđelković, B., Tešević, V.,& Milosavljević, S.. (2022). Analysis of diterpenoids from the latex of E. seguieriana Neck. subsp. seguieriana by liquid chromatography–electrospray ionisation mass spectrometry. in Book of Abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF), 7–9 September 2022, Belgrade, Serbia
Belgrade : University of Belgrade - Faculty of Agriculture., 86-86.
https://hdl.handle.net/21.15107/rcub_cer_5864

Jadranin M, Krstić G, Novaković M, Anđelković B, Tešević V, Milosavljević S. Analysis of diterpenoids from the latex of E. seguieriana Neck. subsp. seguieriana by liquid chromatography–electrospray ionisation mass spectrometry. in Book of Abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF), 7–9 September 2022, Belgrade, Serbia. 2022;:86-86.
https://hdl.handle.net/21.15107/rcub_cer_5864 .

Jadranin, Milka, Krstić, Gordana, Novaković, Miroslav, Anđelković, Boban, Tešević, Vele, Milosavljević, Slobodan, "Analysis of diterpenoids from the latex of E. seguieriana Neck. subsp. seguieriana by liquid chromatography–electrospray ionisation mass spectrometry" in Book of Abstracts - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF), 7–9 September 2022, Belgrade, Serbia (2022):86-86,
https://hdl.handle.net/21.15107/rcub_cer_5864 .

TY  - CONF
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5875
AB  - Plant species of the genus Euphorbia have an important place in the traditional medicine due to the secondary metabolites which they produce. The most important metabolites  come from the classes of diterpenes and triterpenes. The studies have shown that these  compounds can have antitumour, antiviral, antibacterial, anti-inflammatory and other  properties. The aim of the research was to investigatethe triterpene composition of  Euphorbia palustris. Using chromatographic techniques, four triterpene derivatives were  isolated from the plant material, as follows: (+)-tirucallol (1), lanosterol (2), 27-nor-3βhydroxycycloart-23-en-25-one (3) and (+)-obtusifoliol (4). In the continuation of the  research, their biological activities will be examined.
AB  - Biljne vrste roda Euphorbia imaju važno mesto u tradicionalnoj medicini zahvaljujući  sekundarnim metabolitima koje proizvode. Najznačajniji metaboliti pripadaju klasama  diterpena i triterpena. Istraživanja su pokazala da ova jedinjenja imaju antitumorska,  antivirusna, antibakterijska, antiinflamatorna i druga svojstva. Cilj ovog istraživanja bio je  ispitivanje triterpenskog sastava biljne vrste Euphorbia palustris. Primenom  hromatografskih tehnika iz biljnog materijala izolovana su četiri triterpenska derivata i  to:(+)-tirukalol (1), lanosterol (2), 27-nor-3β-hidroksicikloart-23-en-25-on (3) i (+)- obtusifoliol (4). U nastavku istraživanja biće ispitane njihove biološke aktivnosti.
PB  - Serbian Chemical Society
C3  - Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
T1  - Triterpene composition of the plant species Euphorbia palustris
T1  - Triterpeni biljne vrste Euphorbia palustris
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_cer_5875
ER  - 

@conference{
author = "Savić, Danica and Krstić, Gordana and Jadranin, Milka and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "Plant species of the genus Euphorbia have an important place in the traditional medicine due to the secondary metabolites which they produce. The most important metabolites  come from the classes of diterpenes and triterpenes. The studies have shown that these  compounds can have antitumour, antiviral, antibacterial, anti-inflammatory and other  properties. The aim of the research was to investigatethe triterpene composition of  Euphorbia palustris. Using chromatographic techniques, four triterpene derivatives were  isolated from the plant material, as follows: (+)-tirucallol (1), lanosterol (2), 27-nor-3βhydroxycycloart-23-en-25-one (3) and (+)-obtusifoliol (4). In the continuation of the  research, their biological activities will be examined., Biljne vrste roda Euphorbia imaju važno mesto u tradicionalnoj medicini zahvaljujući  sekundarnim metabolitima koje proizvode. Najznačajniji metaboliti pripadaju klasama  diterpena i triterpena. Istraživanja su pokazala da ova jedinjenja imaju antitumorska,  antivirusna, antibakterijska, antiinflamatorna i druga svojstva. Cilj ovog istraživanja bio je  ispitivanje triterpenskog sastava biljne vrste Euphorbia palustris. Primenom  hromatografskih tehnika iz biljnog materijala izolovana su četiri triterpenska derivata i  to:(+)-tirukalol (1), lanosterol (2), 27-nor-3β-hidroksicikloart-23-en-25-on (3) i (+)- obtusifoliol (4). U nastavku istraživanja biće ispitane njihove biološke aktivnosti.",
publisher = "Serbian Chemical Society",
journal = "Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine",
title = "Triterpene composition of the plant species Euphorbia palustris, Triterpeni biljne vrste Euphorbia palustris",
pages = "143",
url = "https://hdl.handle.net/21.15107/rcub_cer_5875"
}

Savić, D., Krstić, G., Jadranin, M., Tešević, V.,& Milosavljević, S.. (2022). Triterpene composition of the plant species Euphorbia palustris. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
Serbian Chemical Society., 143.
https://hdl.handle.net/21.15107/rcub_cer_5875

Savić D, Krstić G, Jadranin M, Tešević V, Milosavljević S. Triterpene composition of the plant species Euphorbia palustris. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine. 2022;:143.
https://hdl.handle.net/21.15107/rcub_cer_5875 .

Savić, Danica, Krstić, Gordana, Jadranin, Milka, Tešević, Vele, Milosavljević, Slobodan, "Triterpene composition of the plant species Euphorbia palustris" in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine (2022):143,
https://hdl.handle.net/21.15107/rcub_cer_5875 .

TY  - JOUR
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Lekić, Stefan
AU  - Jadranin, Milka
AU  - Vujisić, Ljubodrag
AU  - Gođevac, Dejan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5294
AB  - Allium ursinum and poisonous adulterants Convallaria majalis and Arum maculatum were used as a model for detection of adulterants in edible plant. A. ursinum samples were spiked with C. majalis and A. maculatum to mimic adulteration. Metabolomic fingerprinting of all samples was performed using 1H NMR spectroscopy, and the resulting data sets were subjected to multivariate data analysis. As a result of this analysis, signals of adulterants were extracted from the data, and the structures of biomarkers of adulteration from partially purified samples were elucidated using 2D NMR and LC-MS techniques. Thus, isovitexin and vicenin II, azetidine-2-carboxylic acid, and trigonelline indicated adulteration of A. ursinum samples with C. majalis. Isovitexin was also recognized to be an indicator of adulteration of A. ursinum with A. maculatum. In conclusion, the case study of A. ursinum suggested that plant metabolomics approach could be utilized for identification of low molecular weight biomarkers of adulteration in edible plants.
PB  - MDPI
T2  - Metabolites
T1  - Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum
VL  - 12
IS  - 9
SP  - 849
DO  - 10.3390/metabo12090849
ER  - 

@article{
author = "Ivanović, Stefan and Simić, Katarina and Lekić, Stefan and Jadranin, Milka and Vujisić, Ljubodrag and Gođevac, Dejan",
year = "2022",
abstract = "Allium ursinum and poisonous adulterants Convallaria majalis and Arum maculatum were used as a model for detection of adulterants in edible plant. A. ursinum samples were spiked with C. majalis and A. maculatum to mimic adulteration. Metabolomic fingerprinting of all samples was performed using 1H NMR spectroscopy, and the resulting data sets were subjected to multivariate data analysis. As a result of this analysis, signals of adulterants were extracted from the data, and the structures of biomarkers of adulteration from partially purified samples were elucidated using 2D NMR and LC-MS techniques. Thus, isovitexin and vicenin II, azetidine-2-carboxylic acid, and trigonelline indicated adulteration of A. ursinum samples with C. majalis. Isovitexin was also recognized to be an indicator of adulteration of A. ursinum with A. maculatum. In conclusion, the case study of A. ursinum suggested that plant metabolomics approach could be utilized for identification of low molecular weight biomarkers of adulteration in edible plants.",
publisher = "MDPI",
journal = "Metabolites",
title = "Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum",
volume = "12",
number = "9",
pages = "849",
doi = "10.3390/metabo12090849"
}

Ivanović, S., Simić, K., Lekić, S., Jadranin, M., Vujisić, L.,& Gođevac, D.. (2022). Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum. in Metabolites
MDPI., 12(9), 849.
https://doi.org/10.3390/metabo12090849

Ivanović S, Simić K, Lekić S, Jadranin M, Vujisić L, Gođevac D. Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum. in Metabolites. 2022;12(9):849.
doi:10.3390/metabo12090849 .

Ivanović, Stefan, Simić, Katarina, Lekić, Stefan, Jadranin, Milka, Vujisić, Ljubodrag, Gođevac, Dejan, "Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum" in Metabolites, 12, no. 9 (2022):849,
https://doi.org/10.3390/metabo12090849 . .

TY  - JOUR
AU  - Tasic, Ljubica
AU  - Avramović, Nataša
AU  - Jadranin, Milka
AU  - Quintero, Melissa
AU  - Stanisic, Danijela
AU  - Martins, Lucas G.
AU  - Costa, Tássia Brena Barroso Carneiro
AU  - Novak, Estela
AU  - Odone, Vicente
AU  - Rivas, Maria
AU  - Aguiar, Talita
AU  - Carraro, Dirce Maria
AU  - Werneck da Cunha, Isabela
AU  - Lima da Costa, Cecilia Maria
AU  - Maschietto, Mariana
AU  - Krepischi, Ana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5568
AB  - Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.
PB  - MDPI AG
T2  - Biomedicines
T1  - High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks
VL  - 10
IS  - 12
SP  - 3091
DO  - 10.3390/biomedicines10123091
ER  - 

@article{
author = "Tasic, Ljubica and Avramović, Nataša and Jadranin, Milka and Quintero, Melissa and Stanisic, Danijela and Martins, Lucas G. and Costa, Tássia Brena Barroso Carneiro and Novak, Estela and Odone, Vicente and Rivas, Maria and Aguiar, Talita and Carraro, Dirce Maria and Werneck da Cunha, Isabela and Lima da Costa, Cecilia Maria and Maschietto, Mariana and Krepischi, Ana",
year = "2022",
abstract = "Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.",
publisher = "MDPI AG",
journal = "Biomedicines",
title = "High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks",
volume = "10",
number = "12",
pages = "3091",
doi = "10.3390/biomedicines10123091"
}

Tasic, L., Avramović, N., Jadranin, M., Quintero, M., Stanisic, D., Martins, L. G., Costa, T. B. B. C., Novak, E., Odone, V., Rivas, M., Aguiar, T., Carraro, D. M., Werneck da Cunha, I., Lima da Costa, C. M., Maschietto, M.,& Krepischi, A.. (2022). High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks. in Biomedicines
MDPI AG., 10(12), 3091.
https://doi.org/10.3390/biomedicines10123091

Tasic L, Avramović N, Jadranin M, Quintero M, Stanisic D, Martins LG, Costa TBBC, Novak E, Odone V, Rivas M, Aguiar T, Carraro DM, Werneck da Cunha I, Lima da Costa CM, Maschietto M, Krepischi A. High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks. in Biomedicines. 2022;10(12):3091.
doi:10.3390/biomedicines10123091 .

Tasic, Ljubica, Avramović, Nataša, Jadranin, Milka, Quintero, Melissa, Stanisic, Danijela, Martins, Lucas G., Costa, Tássia Brena Barroso Carneiro, Novak, Estela, Odone, Vicente, Rivas, Maria, Aguiar, Talita, Carraro, Dirce Maria, Werneck da Cunha, Isabela, Lima da Costa, Cecilia Maria, Maschietto, Mariana, Krepischi, Ana, "High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks" in Biomedicines, 10, no. 12 (2022):3091,
https://doi.org/10.3390/biomedicines10123091 . .

TY  - JOUR
AU  - Tasić, Ljubica
AU  - Avramović, Nataša
AU  - Quintero, Melissa
AU  - Stanišić, Danijela
AU  - Martins, Lucas
AU  - Brena da Costa, Tássia
AU  - Jadranin, Milka
AU  - Accioly de Souza, Maria Theresa
AU  - Faria, Paulo
AU  - de Camargo, Beatriz
AU  - de Sá Pereira, Bruna Maria
AU  - Maschietto, Mariana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5323
AB  - Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.
PB  - MDPI
T2  - Diagnostic
T1  - A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy
VL  - 12
IS  - 1
SP  - 2
EP  - 10
DO  - 10.3390/diagnostics12010157
ER  - 

@article{
author = "Tasić, Ljubica and Avramović, Nataša and Quintero, Melissa and Stanišić, Danijela and Martins, Lucas and Brena da Costa, Tássia and Jadranin, Milka and Accioly de Souza, Maria Theresa and Faria, Paulo and de Camargo, Beatriz and de Sá Pereira, Bruna Maria and Maschietto, Mariana",
year = "2022",
abstract = "Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.",
publisher = "MDPI",
journal = "Diagnostic",
title = "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy",
volume = "12",
number = "1",
pages = "2-10",
doi = "10.3390/diagnostics12010157"
}

Tasić, L., Avramović, N., Quintero, M., Stanišić, D., Martins, L., Brena da Costa, T., Jadranin, M., Accioly de Souza, M. T., Faria, P., de Camargo, B., de Sá Pereira, B. M.,& Maschietto, M.. (2022). A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic
MDPI., 12(1), 2-10.
https://doi.org/10.3390/diagnostics12010157

Tasić L, Avramović N, Quintero M, Stanišić D, Martins L, Brena da Costa T, Jadranin M, Accioly de Souza MT, Faria P, de Camargo B, de Sá Pereira BM, Maschietto M. A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic. 2022;12(1):2-10.
doi:10.3390/diagnostics12010157 .

Tasić, Ljubica, Avramović, Nataša, Quintero, Melissa, Stanišić, Danijela, Martins, Lucas, Brena da Costa, Tássia, Jadranin, Milka, Accioly de Souza, Maria Theresa, Faria, Paulo, de Camargo, Beatriz, de Sá Pereira, Bruna Maria, Maschietto, Mariana, "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy" in Diagnostic, 12, no. 1 (2022):2-10,
https://doi.org/10.3390/diagnostics12010157 . .

TY  - JOUR
AU  - Milosavljević, Saša
AU  - Jadranin, Milka
AU  - Mladenović, Mića
AU  - Tešević, Vele
AU  - Menković, Nebojša
AU  - Mutavdžić, Dragoslav
AU  - Krstić, Gordana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4681
AB  - The aim of this research was to verify the authenticity of monofloral honeys from the territory of the Republic of Serbia on the basis of physicochemical parameters routinely measured in honey quality control using multivariate analysis. Seventeen samples of monofloral honey (11 samples of acacia honey and 6 samples of sunflower honey) from the territory of the Republic of Serbia were analyzed. Physico-chemical analysis of the samples included the examination of basic quality parameters and qualitative and quantitative analyses of phenolic compounds. In the samples tested, a total of 93 phenolic compounds were tentatively identified, and 19 of them were quantified. The obtained physicochemical analysis of the data served as input for the multivariate analysis. The hеаt map, which is useful for visualizing numerical data, was used for this purpose. The obtained results showed that the applied data can serve to clearly separate acacia and sunflower honeys.
AB  - Целта на ова истражување е да се потврди автентичноста на моноцветен мед од територијата на Република Србија врз основа на физичкохемиските параметри што рутински се мерат при контрола на квалитет на мед со примена на мултиваријантна анализа. Беа анализирани 17 примероци на моноцветен мед (11 примероци мед од акација и 6 примероци мед од сончоглед) од територијата на Република Србија. Физичкохемиските анализа на примероците вклучуваа испитување на основните параметри за квалитет како и квалитативна и квантитативна анализа на фенолните соединенија. Во примероците што беа тестирани, хипотетички беа идентификувани вкупно 93 фенолни соединенија, а 19 од нив беа и квантификувани. Добиените податоци од физичкохемиска анализа на овие податоци беа употребени како влезна податоци за мултиваријантна анализа. Беше применета топлинска мапа која е корисна за визуализација на нумеричките податоци. Добиените резултати покажуваат дека применетите податоци може да послужат за јасно да се раздели медот од акација и од сончоглед.
PB  - North Macedonia : Society of Chemists and Technologists of Macedonia
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Phytochemical parameters as indicators of the authenticity of monofloral honey from the territory of the Republic of Serbia
T1  - Физичкохемијски параметри како индикатори на автентичноста на монофлорален мед од територијата на Република Србија
VL  - 40
IS  - 1
DO  - 10.20450/mjcce.2021.2186
ER  - 

@article{
author = "Milosavljević, Saša and Jadranin, Milka and Mladenović, Mića and Tešević, Vele and Menković, Nebojša and Mutavdžić, Dragoslav and Krstić, Gordana",
year = "2021",
abstract = "The aim of this research was to verify the authenticity of monofloral honeys from the territory of the Republic of Serbia on the basis of physicochemical parameters routinely measured in honey quality control using multivariate analysis. Seventeen samples of monofloral honey (11 samples of acacia honey and 6 samples of sunflower honey) from the territory of the Republic of Serbia were analyzed. Physico-chemical analysis of the samples included the examination of basic quality parameters and qualitative and quantitative analyses of phenolic compounds. In the samples tested, a total of 93 phenolic compounds were tentatively identified, and 19 of them were quantified. The obtained physicochemical analysis of the data served as input for the multivariate analysis. The hеаt map, which is useful for visualizing numerical data, was used for this purpose. The obtained results showed that the applied data can serve to clearly separate acacia and sunflower honeys., Целта на ова истражување е да се потврди автентичноста на моноцветен мед од територијата на Република Србија врз основа на физичкохемиските параметри што рутински се мерат при контрола на квалитет на мед со примена на мултиваријантна анализа. Беа анализирани 17 примероци на моноцветен мед (11 примероци мед од акација и 6 примероци мед од сончоглед) од територијата на Република Србија. Физичкохемиските анализа на примероците вклучуваа испитување на основните параметри за квалитет како и квалитативна и квантитативна анализа на фенолните соединенија. Во примероците што беа тестирани, хипотетички беа идентификувани вкупно 93 фенолни соединенија, а 19 од нив беа и квантификувани. Добиените податоци од физичкохемиска анализа на овие податоци беа употребени како влезна податоци за мултиваријантна анализа. Беше применета топлинска мапа која е корисна за визуализација на нумеричките податоци. Добиените резултати покажуваат дека применетите податоци може да послужат за јасно да се раздели медот од акација и од сончоглед.",
publisher = "North Macedonia : Society of Chemists and Technologists of Macedonia",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Phytochemical parameters as indicators of the authenticity of monofloral honey from the territory of the Republic of Serbia, Физичкохемијски параметри како индикатори на автентичноста на монофлорален мед од територијата на Република Србија",
volume = "40",
number = "1",
doi = "10.20450/mjcce.2021.2186"
}

Milosavljević, S., Jadranin, M., Mladenović, M., Tešević, V., Menković, N., Mutavdžić, D.,& Krstić, G.. (2021). Phytochemical parameters as indicators of the authenticity of monofloral honey from the territory of the Republic of Serbia. in Macedonian Journal of Chemistry and Chemical Engineering
North Macedonia : Society of Chemists and Technologists of Macedonia., 40(1).
https://doi.org/10.20450/mjcce.2021.2186

Milosavljević S, Jadranin M, Mladenović M, Tešević V, Menković N, Mutavdžić D, Krstić G. Phytochemical parameters as indicators of the authenticity of monofloral honey from the territory of the Republic of Serbia. in Macedonian Journal of Chemistry and Chemical Engineering. 2021;40(1).
doi:10.20450/mjcce.2021.2186 .

Milosavljević, Saša, Jadranin, Milka, Mladenović, Mića, Tešević, Vele, Menković, Nebojša, Mutavdžić, Dragoslav, Krstić, Gordana, "Phytochemical parameters as indicators of the authenticity of monofloral honey from the territory of the Republic of Serbia" in Macedonian Journal of Chemistry and Chemical Engineering, 40, no. 1 (2021),
https://doi.org/10.20450/mjcce.2021.2186 . .

TY  - JOUR
AU  - Krstić, Gordana
AU  - Kostić, Ana
AU  - Jadranin, Milka
AU  - Pešić, Milica
AU  - Novaković, Miroslav
AU  - Aljančić, Ivana
AU  - Vajs, Vlatka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4910
AB  - In the previous study fifteen jatrophane diterpenes were isolated from the Euphorbia nicaeensis latex. Fourteen of them have been shown to be potent P-glycoprotein (P-gp) inhibitor in two MDR cancer cells (NCI-H460/R and DLD1-TxR). The aim of this study was to determine whether and which jatro­phane diterpenes can be isolated from the root of the plant, and then to examine their inhibition power on P-glycoprotein of selected cancer cell lines (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR and U87-TxR). Two previously undes­cribed jatrophane diterpenes were isolated from the root of E. nicaeensis col­lected in Deliblato Sand (Serbia). The structures of the isolated compounds were determined using 1D and 2D NMR, as well as HRESIMS data. The results obtained by MTT assay showed different antitumor potential of these two jatrophanes. Compound 1 inhibited cell growth of non-small cell lung car­cinoma cell lines NCI-H460 and NCI-H460/R, as well as glioblastoma cell lines U87 and U87-TxR, while jatrophane 2 was almost completely inactive in the suppression of cancer cell growth in a given range of concentrations. The obtained results also showed that the isolated compounds have an inhibitory effect on P-glycoprotein, as well as that their inhibitory potential is similar.
AB  - У претходном истраживању, петнаест дитерпена јатрофанског типа изоловано је из латекса Euphorbia nicaeensis. Њих четрнаест показала су се као снажни инхибитори P-гликопротеина (P-gp) у две MDR ћелијске линије рака (NCI-H460/R и DLD1-TxR). Циљ ове студије био је да се утврди да ли је и које јатрофанске дитерпене могуће изо- ловати из корена биљке, а затим испитивање њихове инхибиторне моћи на P-гликопро- теину одабраних ћелијских линија рака (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR и U87-TxR). Два претходно непозната јатрофана изолованa су из корена E. nicaeensis прикупљеног у Делиблатској пешчари. Структуре изолованих једињења одређене су применом 1D и 2D NMR метода, као и HRESIMS експеримента. Резултати добијени МТТ тестом показали су различит антиканцерогени потенцијал ова два јатрофана. Једињење 1 је инхибирало раст ћелија ћелијских линија неситноћелијског карцинома плућа NCI-H460 и NCI-H460/R, као и ћелијских линија глиобластома U87 и U87-TxR, док је јатрофан 2 био готово потпуно неефикасан у сузбијању растa ћелија карцинома у датом концентрационом опсегу. Добијени резултати су такође показали да 1 и 2 имају инхибиторно дејство на P-гликопротеин, као и да је њихов инхибиторни потенцијал сличан.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis
T1  - Два нова јатрофанска дитерпена из корена Euphorbia nicaeensis
IS  - 12
SP  - 1219
EP  - 1228
DO  - 10.2298/JSC210806085K
ER  - 

@article{
author = "Krstić, Gordana and Kostić, Ana and Jadranin, Milka and Pešić, Milica and Novaković, Miroslav and Aljančić, Ivana and Vajs, Vlatka",
year = "2021",
abstract = "In the previous study fifteen jatrophane diterpenes were isolated from the Euphorbia nicaeensis latex. Fourteen of them have been shown to be potent P-glycoprotein (P-gp) inhibitor in two MDR cancer cells (NCI-H460/R and DLD1-TxR). The aim of this study was to determine whether and which jatro­phane diterpenes can be isolated from the root of the plant, and then to examine their inhibition power on P-glycoprotein of selected cancer cell lines (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR and U87-TxR). Two previously undes­cribed jatrophane diterpenes were isolated from the root of E. nicaeensis col­lected in Deliblato Sand (Serbia). The structures of the isolated compounds were determined using 1D and 2D NMR, as well as HRESIMS data. The results obtained by MTT assay showed different antitumor potential of these two jatrophanes. Compound 1 inhibited cell growth of non-small cell lung car­cinoma cell lines NCI-H460 and NCI-H460/R, as well as glioblastoma cell lines U87 and U87-TxR, while jatrophane 2 was almost completely inactive in the suppression of cancer cell growth in a given range of concentrations. The obtained results also showed that the isolated compounds have an inhibitory effect on P-glycoprotein, as well as that their inhibitory potential is similar., У претходном истраживању, петнаест дитерпена јатрофанског типа изоловано је из латекса Euphorbia nicaeensis. Њих четрнаест показала су се као снажни инхибитори P-гликопротеина (P-gp) у две MDR ћелијске линије рака (NCI-H460/R и DLD1-TxR). Циљ ове студије био је да се утврди да ли је и које јатрофанске дитерпене могуће изо- ловати из корена биљке, а затим испитивање њихове инхибиторне моћи на P-гликопро- теину одабраних ћелијских линија рака (NCI-H460, DLD1, U87, NCI-H460/R, DLD1-TxR и U87-TxR). Два претходно непозната јатрофана изолованa су из корена E. nicaeensis прикупљеног у Делиблатској пешчари. Структуре изолованих једињења одређене су применом 1D и 2D NMR метода, као и HRESIMS експеримента. Резултати добијени МТТ тестом показали су различит антиканцерогени потенцијал ова два јатрофана. Једињење 1 је инхибирало раст ћелија ћелијских линија неситноћелијског карцинома плућа NCI-H460 и NCI-H460/R, као и ћелијских линија глиобластома U87 и U87-TxR, док је јатрофан 2 био готово потпуно неефикасан у сузбијању растa ћелија карцинома у датом концентрационом опсегу. Добијени резултати су такође показали да 1 и 2 имају инхибиторно дејство на P-гликопротеин, као и да је њихов инхибиторни потенцијал сличан.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis, Два нова јатрофанска дитерпена из корена Euphorbia nicaeensis",
number = "12",
pages = "1219-1228",
doi = "10.2298/JSC210806085K"
}

Krstić, G., Kostić, A., Jadranin, M., Pešić, M., Novaković, M., Aljančić, I.,& Vajs, V.. (2021). Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis. in Journal of the Serbian Chemical Society
Serbian Chemical Society.(12), 1219-1228.
https://doi.org/10.2298/JSC210806085K

Krstić G, Kostić A, Jadranin M, Pešić M, Novaković M, Aljančić I, Vajs V. Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis. in Journal of the Serbian Chemical Society. 2021;(12):1219-1228.
doi:10.2298/JSC210806085K .

Krstić, Gordana, Kostić, Ana, Jadranin, Milka, Pešić, Milica, Novaković, Miroslav, Aljančić, Ivana, Vajs, Vlatka, "Two new jatrophane diterpenes from the roots of Euphorbia nicaeensis" in Journal of the Serbian Chemical Society, no. 12 (2021):1219-1228,
https://doi.org/10.2298/JSC210806085K . .

TY  - CONF
AU  - Tasić, Ljubica
AU  - Martins, Lucas
AU  - Braga, Erik Sobrinho
AU  - Novais, M.
AU  - Jadranin, Milka
AU  - Stanišić, Danijela
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7277
AB  - The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.
PB  - NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)
C3  - 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
T1  - Impressions on COVID-19 by NMR
SP  - OC24
UR  - https://hdl.handle.net/21.15107/rcub_cer_7277
ER  - 

@conference{
author = "Tasić, Ljubica and Martins, Lucas and Braga, Erik Sobrinho and Novais, M. and Jadranin, Milka and Stanišić, Danijela",
year = "2021",
abstract = "The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.",
publisher = "NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)",
journal = "10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event",
title = "Impressions on COVID-19 by NMR",
pages = "OC24",
url = "https://hdl.handle.net/21.15107/rcub_cer_7277"
}

Tasić, L., Martins, L., Braga, E. S., Novais, M., Jadranin, M.,& Stanišić, D.. (2021). Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)., OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277

Tasić L, Martins L, Braga ES, Novais M, Jadranin M, Stanišić D. Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event. 2021;:OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277 .

Tasić, Ljubica, Martins, Lucas, Braga, Erik Sobrinho, Novais, M., Jadranin, Milka, Stanišić, Danijela, "Impressions on COVID-19 by NMR" in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event (2021):OC24,
https://hdl.handle.net/21.15107/rcub_cer_7277 .

TY  - JOUR
AU  - Ivanović, Ljubica
AU  - Topalović, Ana
AU  - Bogdanović, Višnja
AU  - Djurović, Dijana
AU  - Mugoša, Boban
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Beškoski, Vladimir
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4797
AB  - Purpose: The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. Design/methodology/approach: Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines. Findings: The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively. Originality/value: These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.
PB  - Emerald Publishing Limited
T2  - British Food Journal
T1  - Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes
VL  - 123
IS  - 7
SP  - 2335
EP  - 2348
DO  - 10.1108/BFJ-11-2020-1062
ER  - 

@article{
author = "Ivanović, Ljubica and Topalović, Ana and Bogdanović, Višnja and Djurović, Dijana and Mugoša, Boban and Jadranin, Milka and Tešević, Vele and Beškoski, Vladimir",
year = "2021",
abstract = "Purpose: The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. Design/methodology/approach: Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines. Findings: The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively. Originality/value: These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.",
publisher = "Emerald Publishing Limited",
journal = "British Food Journal",
title = "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes",
volume = "123",
number = "7",
pages = "2335-2348",
doi = "10.1108/BFJ-11-2020-1062"
}

Ivanović, L., Topalović, A., Bogdanović, V., Djurović, D., Mugoša, B., Jadranin, M., Tešević, V.,& Beškoski, V.. (2021). Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. in British Food Journal
Emerald Publishing Limited., 123(7), 2335-2348.
https://doi.org/10.1108/BFJ-11-2020-1062

Ivanović L, Topalović A, Bogdanović V, Djurović D, Mugoša B, Jadranin M, Tešević V, Beškoski V. Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes. in British Food Journal. 2021;123(7):2335-2348.
doi:10.1108/BFJ-11-2020-1062 .

Ivanović, Ljubica, Topalović, Ana, Bogdanović, Višnja, Djurović, Dijana, Mugoša, Boban, Jadranin, Milka, Tešević, Vele, Beškoski, Vladimir, "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes" in British Food Journal, 123, no. 7 (2021):2335-2348,
https://doi.org/10.1108/BFJ-11-2020-1062 . .

TY  - JOUR
AU  - Đorđević, Iris
AU  - Cvetković, Mirjana
AU  - Jadranin, Milka
AU  - Bojović, Srdjan
AU  - Andjelković, Boban
AU  - Sofrenić, Ivana
AU  - Simić, Katarina
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4907
AB  - Phytochemistry deals with the study of secondary metabolites pro­duced by plants that synthesize these compounds for many reasons, including their own protection against attack of herbivores and plant diseases. Secondary metabolites are believed to represent plant adaptation to various environmental factors and that they enabled the survival of the species. Secondary metabolites of plants can have curative or toxic effects in humans and animals. Herbal medicine has a long tradition in folk medicine and until the early 20th century, when synthetic organic chemistry began to develop, plants were the main source of medicines. The two basic goals of our phytochemical research are: isolation and identification of new (biologically active) compounds – potential drugs, and chemotaxonomy (chemo­systematics). In the following text through one selected example, the genus Amphoricarpos Vis., our phytochemical res­earch is shown on both aspects.
AB  - Фитохемија се бави изучавањем секундарних метаболита кoje биљке синтетишу из много разлога, укључујући сопствену заштиту од напада биљоједа и од биљних болести. Сматра се да секундарни метаболити представљају адаптацију биљака на различите факторе околине и да су управо они омогућили опстанак врста. Секундарни метаболити биљака могу испољити лековито или токсично дејство на људе и животиње. Лечење биљем има дугу традицију у народној медицини и све до почетка 20. века, када је почела да се развија синтетичка органска хемија, биљке су биле главни извор лекова. Основни циљеви наших фитохемијских истраживања обухватају: изоловање и идентификацију нових (биолошки активних) једињења – потенцијалних лекова, и хемотаксономију (хемосистематика). У тексту су кроз један одабран пример – род Amphoricarpos Vis. – приказана оба наведена аспекта наших фитохемијских истраживања.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Phytochemical study of the genus amphoricarpos
T1  - Фитохемијско истраживање биљног рода Amphoricarpos
VL  - 86
IS  - 12
SP  - 1177
EP  - 1193
DO  - 10.2298/JSC210726083D
ER  - 

@article{
author = "Đorđević, Iris and Cvetković, Mirjana and Jadranin, Milka and Bojović, Srdjan and Andjelković, Boban and Sofrenić, Ivana and Simić, Katarina",
year = "2021",
abstract = "Phytochemistry deals with the study of secondary metabolites pro­duced by plants that synthesize these compounds for many reasons, including their own protection against attack of herbivores and plant diseases. Secondary metabolites are believed to represent plant adaptation to various environmental factors and that they enabled the survival of the species. Secondary metabolites of plants can have curative or toxic effects in humans and animals. Herbal medicine has a long tradition in folk medicine and until the early 20th century, when synthetic organic chemistry began to develop, plants were the main source of medicines. The two basic goals of our phytochemical research are: isolation and identification of new (biologically active) compounds – potential drugs, and chemotaxonomy (chemo­systematics). In the following text through one selected example, the genus Amphoricarpos Vis., our phytochemical res­earch is shown on both aspects., Фитохемија се бави изучавањем секундарних метаболита кoje биљке синтетишу из много разлога, укључујући сопствену заштиту од напада биљоједа и од биљних болести. Сматра се да секундарни метаболити представљају адаптацију биљака на различите факторе околине и да су управо они омогућили опстанак врста. Секундарни метаболити биљака могу испољити лековито или токсично дејство на људе и животиње. Лечење биљем има дугу традицију у народној медицини и све до почетка 20. века, када је почела да се развија синтетичка органска хемија, биљке су биле главни извор лекова. Основни циљеви наших фитохемијских истраживања обухватају: изоловање и идентификацију нових (биолошки активних) једињења – потенцијалних лекова, и хемотаксономију (хемосистематика). У тексту су кроз један одабран пример – род Amphoricarpos Vis. – приказана оба наведена аспекта наших фитохемијских истраживања.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Phytochemical study of the genus amphoricarpos, Фитохемијско истраживање биљног рода Amphoricarpos",
volume = "86",
number = "12",
pages = "1177-1193",
doi = "10.2298/JSC210726083D"
}

Đorđević, I., Cvetković, M., Jadranin, M., Bojović, S., Andjelković, B., Sofrenić, I.,& Simić, K.. (2021). Phytochemical study of the genus amphoricarpos. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 86(12), 1177-1193.
https://doi.org/10.2298/JSC210726083D

Đorđević I, Cvetković M, Jadranin M, Bojović S, Andjelković B, Sofrenić I, Simić K. Phytochemical study of the genus amphoricarpos. in Journal of the Serbian Chemical Society. 2021;86(12):1177-1193.
doi:10.2298/JSC210726083D .

Đorđević, Iris, Cvetković, Mirjana, Jadranin, Milka, Bojović, Srdjan, Andjelković, Boban, Sofrenić, Ivana, Simić, Katarina, "Phytochemical study of the genus amphoricarpos" in Journal of the Serbian Chemical Society, 86, no. 12 (2021):1177-1193,
https://doi.org/10.2298/JSC210726083D . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Jadranin, Milka
AU  - Przić, Zoran
AU  - Marković, Nebojša
AU  - Sokolović, Danijel
AU  - Savikin, Katarina
AU  - Menković, Nebojša
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4249
AB  - In this study we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC‐MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of cumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.
T2  - Chemistry & Biodiversity
T1  - Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract
DO  - 10.1002/cbdv.202000858
ER  - 

@article{
author = "Živković, Jelena and Jadranin, Milka and Przić, Zoran and Marković, Nebojša and Sokolović, Danijel and Savikin, Katarina and Menković, Nebojša",
year = "2021",
abstract = "In this study we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC‐MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of cumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.",
journal = "Chemistry & Biodiversity",
title = "Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract",
doi = "10.1002/cbdv.202000858"
}

Živković, J., Jadranin, M., Przić, Z., Marković, N., Sokolović, D., Savikin, K.,& Menković, N.. (2021). Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract. in Chemistry & Biodiversity.
https://doi.org/10.1002/cbdv.202000858

Živković J, Jadranin M, Przić Z, Marković N, Sokolović D, Savikin K, Menković N. Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract. in Chemistry & Biodiversity. 2021;.
doi:10.1002/cbdv.202000858 .

Živković, Jelena, Jadranin, Milka, Przić, Zoran, Marković, Nebojša, Sokolović, Danijel, Savikin, Katarina, Menković, Nebojša, "Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract" in Chemistry & Biodiversity (2021),
https://doi.org/10.1002/cbdv.202000858 . .

TY  - JOUR
AU  - Vučković, Ivan
AU  - Stiković, Sonja
AU  - Stešević, Danijela
AU  - Jadranin, Milka
AU  - Trifunović, Snežana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4919
AB  - The plant species Pimpinella serbica (Vis.) Drude, endemic to West Balkans, belongs to the genus Pimpinella L. (Apiaceae) according to newer botanical classification. Initially, the plant was described as Pancicia serbica Vis. and long considered as a monotypic genus. Previous phytochemical inves­tigations of this plant were limited to the essential oil analysis by the GC–MS method. This is first study that includes LC-DAD–MS screening of the extracts of the roots and aerial parts, followed by isolation of secondary metabolites using chromatographic techniques. Five compounds belonging to phenylpropanoids and polyacetylenes, well known for their bioactivities, were isolated and struc­turally determined by combined spectroscopic methods (UV, NMR, MS). Dil­lapiole (1), nothoapiole (2) and oplopantriol A 18-acetate (4) were found in both extracts, while falcarindiol (3) was isolated from the roots and dendrotrifidol (5) from the aerial parts only. The phytochemical profile of P. serbica L. supports its position in the Pimpinella L. genus.
AB  - Ендемска врста Западног Балкана Pimpinella serbica (Vis.) Drude према последњој ботаничкој класификацији припада роду Pimpinella L. (Apiaceae), мада је најпре била описана као Pancicia serbica Vis. и дуго је сматрана једином припадајућом врстом овог рода. Претходна фитохемијска испитивања ове врсте обухватају само анализу етарског уља GC–MS техником, тако да је ово прво детаљно испитивање биљке које укључује LCDAD– MS анализу праћену изоловањем секундарних метаболита биљке применом хро- матографских техника. Пет једињења која припадају групи фенилпропаноида и поли- ацетилена, добро познатих по својим биолошким активностима, изоловано је из екстра- ката надземног дела и корена биљке. Њихова структура је одређена применом спек- троскопских метода (UV, NMR, MS). Дилапиол (1), нотоапиол (2) и оплопантриол А 18-ацетат (4) нађени су у оба екстракта, док је фалкариндиол (3) изолован само из екс- тракта корена, а дендротрифидол (5) само из екстракта надземног дела биљке. Фито- хемијски профил биљке P. serbica подржава њену припадност роду Pimpinella L.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Phytochemical investigation of Pimpinella serbica
T1  - Фитохемијско испитивање врсте Pimpinella serbica
IS  - 12
SP  - 1241
EP  - 1247
DO  - 10.2298/JSC210816081V
ER  - 

@article{
author = "Vučković, Ivan and Stiković, Sonja and Stešević, Danijela and Jadranin, Milka and Trifunović, Snežana",
year = "2021",
abstract = "The plant species Pimpinella serbica (Vis.) Drude, endemic to West Balkans, belongs to the genus Pimpinella L. (Apiaceae) according to newer botanical classification. Initially, the plant was described as Pancicia serbica Vis. and long considered as a monotypic genus. Previous phytochemical inves­tigations of this plant were limited to the essential oil analysis by the GC–MS method. This is first study that includes LC-DAD–MS screening of the extracts of the roots and aerial parts, followed by isolation of secondary metabolites using chromatographic techniques. Five compounds belonging to phenylpropanoids and polyacetylenes, well known for their bioactivities, were isolated and struc­turally determined by combined spectroscopic methods (UV, NMR, MS). Dil­lapiole (1), nothoapiole (2) and oplopantriol A 18-acetate (4) were found in both extracts, while falcarindiol (3) was isolated from the roots and dendrotrifidol (5) from the aerial parts only. The phytochemical profile of P. serbica L. supports its position in the Pimpinella L. genus., Ендемска врста Западног Балкана Pimpinella serbica (Vis.) Drude према последњој ботаничкој класификацији припада роду Pimpinella L. (Apiaceae), мада је најпре била описана као Pancicia serbica Vis. и дуго је сматрана једином припадајућом врстом овог рода. Претходна фитохемијска испитивања ове врсте обухватају само анализу етарског уља GC–MS техником, тако да је ово прво детаљно испитивање биљке које укључује LCDAD– MS анализу праћену изоловањем секундарних метаболита биљке применом хро- матографских техника. Пет једињења која припадају групи фенилпропаноида и поли- ацетилена, добро познатих по својим биолошким активностима, изоловано је из екстра- ката надземног дела и корена биљке. Њихова структура је одређена применом спек- троскопских метода (UV, NMR, MS). Дилапиол (1), нотоапиол (2) и оплопантриол А 18-ацетат (4) нађени су у оба екстракта, док је фалкариндиол (3) изолован само из екс- тракта корена, а дендротрифидол (5) само из екстракта надземног дела биљке. Фито- хемијски профил биљке P. serbica подржава њену припадност роду Pimpinella L.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Phytochemical investigation of Pimpinella serbica, Фитохемијско испитивање врсте Pimpinella serbica",
number = "12",
pages = "1241-1247",
doi = "10.2298/JSC210816081V"
}

Vučković, I., Stiković, S., Stešević, D., Jadranin, M.,& Trifunović, S.. (2021). Phytochemical investigation of Pimpinella serbica. in Journal of the Serbian Chemical Society
Serbian Chemical Society.(12), 1241-1247.
https://doi.org/10.2298/JSC210816081V

Vučković I, Stiković S, Stešević D, Jadranin M, Trifunović S. Phytochemical investigation of Pimpinella serbica. in Journal of the Serbian Chemical Society. 2021;(12):1241-1247.
doi:10.2298/JSC210816081V .

Vučković, Ivan, Stiković, Sonja, Stešević, Danijela, Jadranin, Milka, Trifunović, Snežana, "Phytochemical investigation of Pimpinella serbica" in Journal of the Serbian Chemical Society, no. 12 (2021):1241-1247,
https://doi.org/10.2298/JSC210816081V . .

TY  - CONF
AU  - Stanišić, Danijela
AU  - Jadranin, Milka
AU  - Tasić, Ljubica
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7278
AB  - Identificar metabólitos séricos que refletem o estado e a gravidade da doença pode ajudar no diagnóstico e prognóstico, definir as melhores intervenções a serem aplicadas e o momento correto, bem como compreender o impacto da doença em todo o corpo. Com isso em mente, este estudo se propôs a medir o metabolôma do soro sanguíneo de 37 pacientes com sintomas de COVID-19 grave usando RMN de 1H. Os pacientes que foram positivos para SARS-CoV-2 por RT-qPCR foram agrupados como “COVID-19 grave” (P, vermelho, n = 21, Fig. 1), enquanto os pacientes negativos por RT-qPCR foram agrupados como “não COVID-19 grave” (N, azul, n = 16, Fig. 1). Essas amostras foram comparadas com as amostras de referência de voluntários 
saudáveis (H, verde, n = 40, Fig. 1) retiradas antes de início da pandemia. Os grupos eram homogêneos em relação ao gênero, idade e comorbidades para evitar qualquer comparação tendenciosa. Os soros (200 µL) foram descongelados em gelo, misturados com 250 µL de óxido de deutério (D2O 99,9%) em temperatura ambiente, centrifugados a 12,000 g por 2 min a 4 ° C e transferidos para tubos de RMN de 5 mm. Os metabólitos não polares (lipídios) foram isolados e analisados usando CDCl3 como solvente. Os espectros de alta resolução de RMN de 1H NOESY 1D, CPMG, DOSY editado e em 2D (TOCSY, HSQC) foram adquiridos no espectrômetro Bruker AVANCE III 600 MHz usando a sonda tripla inversa (TBI) a 25 °C. Os três grupos (P, N e H) foram 
separados com facilidade em PCA e em PLSDA (Fig. 1) independentemente da matriz de dados usada. Os dados de RMN de 1H de NOESY, CPMG, editados por DOSY do soro ou de lipídios isolados acusaram diferenças evidentes entre os grupos analisados. Os efeitos da COVID-19 sobre metabólitos séricos foram extremos e refletem todas as respostas do corpo ao vírus SARS-CoV-2. Especialmente os altos níveis de glicose chamam a atenção e a desproporção em níveis e tipos de lipídios. Enquanto o colesterol e os esteróis livres foram muito menores em pacientes com COVID-19 grave do que em controles saudáveis, as fosfocolinas e VLDL foram as mais abundantes. Além disso, a maioria (60-75%) dos metabólitos do soro sanguíneo aumentou em 
pacientes com COVID-19 e em pacientes que foram negativos, porém com sintomas graves. Quando analisadas as vias metabólicas, muitas delas foram alteradas de maneira marcante devido à doença. Como por exemplo a biossíntese de aminoacil t-RNA, metabolismo de aminoácidos (glicina, serina e treonina), metabolismo de aminoácidos de cadeia ramificada (valina, leucina e isoleucina), metabolismo de glicerofosfolipídeos, entre outras vias. Finalmente, a RMN de 1H poderá ser usada com sucesso para classificar os pacientes graves devido múltiplas e várias alterações de metabólitos e níveis de lipídios no seu soro.
PB  - Associação de Usuários de RMN - AUREMN
C3  - XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online
T1  - Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN
UR  - https://hdl.handle.net/21.15107/rcub_cer_7278
ER  - 

@conference{
author = "Stanišić, Danijela and Jadranin, Milka and Tasić, Ljubica",
year = "2020",
abstract = "Identificar metabólitos séricos que refletem o estado e a gravidade da doença pode ajudar no diagnóstico e prognóstico, definir as melhores intervenções a serem aplicadas e o momento correto, bem como compreender o impacto da doença em todo o corpo. Com isso em mente, este estudo se propôs a medir o metabolôma do soro sanguíneo de 37 pacientes com sintomas de COVID-19 grave usando RMN de 1H. Os pacientes que foram positivos para SARS-CoV-2 por RT-qPCR foram agrupados como “COVID-19 grave” (P, vermelho, n = 21, Fig. 1), enquanto os pacientes negativos por RT-qPCR foram agrupados como “não COVID-19 grave” (N, azul, n = 16, Fig. 1). Essas amostras foram comparadas com as amostras de referência de voluntários 
saudáveis (H, verde, n = 40, Fig. 1) retiradas antes de início da pandemia. Os grupos eram homogêneos em relação ao gênero, idade e comorbidades para evitar qualquer comparação tendenciosa. Os soros (200 µL) foram descongelados em gelo, misturados com 250 µL de óxido de deutério (D2O 99,9%) em temperatura ambiente, centrifugados a 12,000 g por 2 min a 4 ° C e transferidos para tubos de RMN de 5 mm. Os metabólitos não polares (lipídios) foram isolados e analisados usando CDCl3 como solvente. Os espectros de alta resolução de RMN de 1H NOESY 1D, CPMG, DOSY editado e em 2D (TOCSY, HSQC) foram adquiridos no espectrômetro Bruker AVANCE III 600 MHz usando a sonda tripla inversa (TBI) a 25 °C. Os três grupos (P, N e H) foram 
separados com facilidade em PCA e em PLSDA (Fig. 1) independentemente da matriz de dados usada. Os dados de RMN de 1H de NOESY, CPMG, editados por DOSY do soro ou de lipídios isolados acusaram diferenças evidentes entre os grupos analisados. Os efeitos da COVID-19 sobre metabólitos séricos foram extremos e refletem todas as respostas do corpo ao vírus SARS-CoV-2. Especialmente os altos níveis de glicose chamam a atenção e a desproporção em níveis e tipos de lipídios. Enquanto o colesterol e os esteróis livres foram muito menores em pacientes com COVID-19 grave do que em controles saudáveis, as fosfocolinas e VLDL foram as mais abundantes. Além disso, a maioria (60-75%) dos metabólitos do soro sanguíneo aumentou em 
pacientes com COVID-19 e em pacientes que foram negativos, porém com sintomas graves. Quando analisadas as vias metabólicas, muitas delas foram alteradas de maneira marcante devido à doença. Como por exemplo a biossíntese de aminoacil t-RNA, metabolismo de aminoácidos (glicina, serina e treonina), metabolismo de aminoácidos de cadeia ramificada (valina, leucina e isoleucina), metabolismo de glicerofosfolipídeos, entre outras vias. Finalmente, a RMN de 1H poderá ser usada com sucesso para classificar os pacientes graves devido múltiplas e várias alterações de metabólitos e níveis de lipídios no seu soro.",
publisher = "Associação de Usuários de RMN - AUREMN",
journal = "XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online",
title = "Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN",
url = "https://hdl.handle.net/21.15107/rcub_cer_7278"
}

Stanišić, D., Jadranin, M.,& Tasić, L.. (2020). Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN. in XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online
Associação de Usuários de RMN - AUREMN..
https://hdl.handle.net/21.15107/rcub_cer_7278

Stanišić D, Jadranin M, Tasić L. Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN. in XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online. 2020;.
https://hdl.handle.net/21.15107/rcub_cer_7278 .

Stanišić, Danijela, Jadranin, Milka, Tasić, Ljubica, "Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN" in XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online (2020),
https://hdl.handle.net/21.15107/rcub_cer_7278 .