TY  - JOUR
AU  - Ilic, Budimir S.
AU  - Kocic, Branislava D.
AU  - Ciric, Vojislav M.
AU  - Cvetković, Olga
AU  - Miladinovic, Dragoljub L.
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1540
AB  - The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.
PB  - Hindawi Publishing Corporation, New York
T2  - Scientific World Journal
T1  - An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol
DO  - 10.1155/2014/826219
ER  - 

@article{
author = "Ilic, Budimir S. and Kocic, Branislava D. and Ciric, Vojislav M. and Cvetković, Olga and Miladinovic, Dragoljub L.",
year = "2014",
abstract = "The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.",
publisher = "Hindawi Publishing Corporation, New York",
journal = "Scientific World Journal",
title = "An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol",
doi = "10.1155/2014/826219"
}

Ilic, B. S., Kocic, B. D., Ciric, V. M., Cvetković, O.,& Miladinovic, D. L.. (2014). An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol. in Scientific World Journal
Hindawi Publishing Corporation, New York..
https://doi.org/10.1155/2014/826219

Ilic BS, Kocic BD, Ciric VM, Cvetković O, Miladinovic DL. An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol. in Scientific World Journal. 2014;.
doi:10.1155/2014/826219 .

Ilic, Budimir S., Kocic, Branislava D., Ciric, Vojislav M., Cvetković, Olga, Miladinovic, Dragoljub L., "An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/826219 . .

TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Mihajilov-Krstev, Tatjana M.
AU  - Nikolic, Dejan M.
AU  - Cvetković, Olga
AU  - Marković, Marija S.
AU  - Miladinovic, Ljiljana C.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1265
AB  - The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 mu g/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 mu g/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.
PB  - Natural Product Incorporation
T2  - Natural Product Communications
T1  - Antibacterial Activity of the Essential Oil of Heracleum sibiricum
VL  - 8
IS  - 9
SP  - 1309
EP  - 1311
UR  - https://hdl.handle.net/21.15107/rcub_cer_1265
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Mihajilov-Krstev, Tatjana M. and Nikolic, Dejan M. and Cvetković, Olga and Marković, Marija S. and Miladinovic, Ljiljana C.",
year = "2013",
abstract = "The composition and antimicrobial activity of the essential oil of Heracleum sibiricum L. (Apiaceae) was studied. The aerial part of plant was hydro-distilled and chemical composition of the essential oil was analyzed by GC and GC-MS. Forty-six compounds, corresponding to 95.12% of the total oil, were identified. Esters represented the major chemical class (69.55%) while the main constituents were octyl butanoate (36.82%), hexyl butanoate (16.08%), 1-octanol (13.62%) and octyl hexanoate (8.10%). Antibacterial activity of the essential oil and reference antibiotics against nine bacterial strains was tested by the broth microdilution method. The results of the bioassays showed that essential oil had slight antimicrobial activities against all tested microorganisms (MIC and MBC values were in the range of 2431.2 to 9724.8 mu g/mL). Reference antibiotics were active in concentrations between 0.5 and 16.0 mu g/mL. The results confirm that Gram-positive bacteria were more susceptible to the essential oil of H. sibiricum, in comparison with Gram-negative bacteria.",
publisher = "Natural Product Incorporation",
journal = "Natural Product Communications",
title = "Antibacterial Activity of the Essential Oil of Heracleum sibiricum",
volume = "8",
number = "9",
pages = "1309-1311",
url = "https://hdl.handle.net/21.15107/rcub_cer_1265"
}

Miladinovic, D. L., Ilic, B. S., Mihajilov-Krstev, T. M., Nikolic, D. M., Cvetković, O., Marković, M. S.,& Miladinovic, L. C.. (2013). Antibacterial Activity of the Essential Oil of Heracleum sibiricum. in Natural Product Communications
Natural Product Incorporation., 8(9), 1309-1311.
https://hdl.handle.net/21.15107/rcub_cer_1265

Miladinovic DL, Ilic BS, Mihajilov-Krstev TM, Nikolic DM, Cvetković O, Marković MS, Miladinovic LC. Antibacterial Activity of the Essential Oil of Heracleum sibiricum. in Natural Product Communications. 2013;8(9):1309-1311.
https://hdl.handle.net/21.15107/rcub_cer_1265 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Mihajilov-Krstev, Tatjana M., Nikolic, Dejan M., Cvetković, Olga, Marković, Marija S., Miladinovic, Ljiljana C., "Antibacterial Activity of the Essential Oil of Heracleum sibiricum" in Natural Product Communications, 8, no. 9 (2013):1309-1311,
https://hdl.handle.net/21.15107/rcub_cer_1265 .

TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Najman, Stevo J.
AU  - Cvetković, Olga
AU  - Šajnović, Aleksandra
AU  - Miladinović, Marija D.
AU  - Nikolic, Nikola D.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1320
AB  - The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.
PB  - Versita, Warsaw
T2  - Central European Journal of Chemistry
T1  - Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract
VL  - 11
IS  - 2
SP  - 123
EP  - 132
DO  - 10.2478/s11532-012-0163-6
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Najman, Stevo J. and Cvetković, Olga and Šajnović, Aleksandra and Miladinović, Marija D. and Nikolic, Nikola D.",
year = "2013",
abstract = "The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Chemistry",
title = "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract",
volume = "11",
number = "2",
pages = "123-132",
doi = "10.2478/s11532-012-0163-6"
}

Miladinovic, D. L., Ilic, B. S., Najman, S. J., Cvetković, O., Šajnović, A., Miladinović, M. D.,& Nikolic, N. D.. (2013). Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. in Central European Journal of Chemistry
Versita, Warsaw., 11(2), 123-132.
https://doi.org/10.2478/s11532-012-0163-6

Miladinovic DL, Ilic BS, Najman SJ, Cvetković O, Šajnović A, Miladinović MD, Nikolic ND. Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. in Central European Journal of Chemistry. 2013;11(2):123-132.
doi:10.2478/s11532-012-0163-6 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Najman, Stevo J., Cvetković, Olga, Šajnović, Aleksandra, Miladinović, Marija D., Nikolic, Nikola D., "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract" in Central European Journal of Chemistry, 11, no. 2 (2013):123-132,
https://doi.org/10.2478/s11532-012-0163-6 . .

TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Mihajilov-Krstev, Tatjana M.
AU  - Nikolic, Nikola D.
AU  - Miladinovic, Ljiljana C.
AU  - Cvetković, Olga
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/954
AB  - The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and beta-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods
VL  - 403
IS  - 4
SP  - 1007
EP  - 1018
DO  - 10.1007/s00216-012-5866-1
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Mihajilov-Krstev, Tatjana M. and Nikolic, Nikola D. and Miladinovic, Ljiljana C. and Cvetković, Olga",
year = "2012",
abstract = "The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and beta-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods",
volume = "403",
number = "4",
pages = "1007-1018",
doi = "10.1007/s00216-012-5866-1"
}

Miladinovic, D. L., Ilic, B. S., Mihajilov-Krstev, T. M., Nikolic, N. D., Miladinovic, L. C.,& Cvetković, O.. (2012). Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 403(4), 1007-1018.
https://doi.org/10.1007/s00216-012-5866-1

Miladinovic DL, Ilic BS, Mihajilov-Krstev TM, Nikolic ND, Miladinovic LC, Cvetković O. Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods. in Analytical and Bioanalytical Chemistry. 2012;403(4):1007-1018.
doi:10.1007/s00216-012-5866-1 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Mihajilov-Krstev, Tatjana M., Nikolic, Nikola D., Miladinovic, Ljiljana C., Cvetković, Olga, "Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods" in Analytical and Bioanalytical Chemistry, 403, no. 4 (2012):1007-1018,
https://doi.org/10.1007/s00216-012-5866-1 . .