TY  - JOUR
AU  - Salomé-Abarca, Luis Francisco
AU  - Gođevac, Dejan
AU  - Sun Kim, Min
AU  - Hwang, Geum-Sook
AU  - Park, Sang Cheol
AU  - Jang, Young Pyo
AU  - Van Den Hondel, Cees A M J J
AU  - Verpoorte, Robert
AU  - Klinkhamer, Peter G L
AU  - Choi, Young Hae
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4576
AB  - Based on the hypothesis that the variation of the metabolomes of latex is a response to selective pressure and should thus be affected differently from other organs, their variation could provide an insight into the defensive chemical selection of plants. Metabolic profiling was used to compare tissues of three Euphorbia species collected in diverse regions. The metabolic variation of latexes was much more limited than that of other organs. In all the species, the levels of polyisoprenes and terpenes were found to be much higher in latexes than in leaves and roots of the corresponding plants. Polyisoprenes were observed to physically delay the contact of pathogens with plant tissues and their growth. A secondary barrier composed of terpenes in latex and in particular, 24-methylenecycloartanol, exhibited antifungal activity. These results added to the well-known role of enzymes also present in latexes, show that these are part of a cooperative defense system comprising biochemical and physical elements.
PB  - Springer
T2  - Journal of Chemical Ecology
T1  - Latex Metabolome of Euphorbia Species: Geographical and Inter-Species Variation and its Proposed Role in Plant Defense against Herbivores and Pathogens
DO  - 10.1007/s10886-021-01274-x
ER  - 

@article{
author = "Salomé-Abarca, Luis Francisco and Gođevac, Dejan and Sun Kim, Min and Hwang, Geum-Sook and Park, Sang Cheol and Jang, Young Pyo and Van Den Hondel, Cees A M J J and Verpoorte, Robert and Klinkhamer, Peter G L and Choi, Young Hae",
year = "2021",
abstract = "Based on the hypothesis that the variation of the metabolomes of latex is a response to selective pressure and should thus be affected differently from other organs, their variation could provide an insight into the defensive chemical selection of plants. Metabolic profiling was used to compare tissues of three Euphorbia species collected in diverse regions. The metabolic variation of latexes was much more limited than that of other organs. In all the species, the levels of polyisoprenes and terpenes were found to be much higher in latexes than in leaves and roots of the corresponding plants. Polyisoprenes were observed to physically delay the contact of pathogens with plant tissues and their growth. A secondary barrier composed of terpenes in latex and in particular, 24-methylenecycloartanol, exhibited antifungal activity. These results added to the well-known role of enzymes also present in latexes, show that these are part of a cooperative defense system comprising biochemical and physical elements.",
publisher = "Springer",
journal = "Journal of Chemical Ecology",
title = "Latex Metabolome of Euphorbia Species: Geographical and Inter-Species Variation and its Proposed Role in Plant Defense against Herbivores and Pathogens",
doi = "10.1007/s10886-021-01274-x"
}

Salomé-Abarca, L. F., Gođevac, D., Sun Kim, M., Hwang, G., Park, S. C., Jang, Y. P., Van Den Hondel, C. A. M. J. J., Verpoorte, R., Klinkhamer, P. G. L.,& Choi, Y. H.. (2021). Latex Metabolome of Euphorbia Species: Geographical and Inter-Species Variation and its Proposed Role in Plant Defense against Herbivores and Pathogens. in Journal of Chemical Ecology
Springer..
https://doi.org/10.1007/s10886-021-01274-x

Salomé-Abarca LF, Gođevac D, Sun Kim M, Hwang G, Park SC, Jang YP, Van Den Hondel CAMJJ, Verpoorte R, Klinkhamer PGL, Choi YH. Latex Metabolome of Euphorbia Species: Geographical and Inter-Species Variation and its Proposed Role in Plant Defense against Herbivores and Pathogens. in Journal of Chemical Ecology. 2021;.
doi:10.1007/s10886-021-01274-x .

Salomé-Abarca, Luis Francisco, Gođevac, Dejan, Sun Kim, Min, Hwang, Geum-Sook, Park, Sang Cheol, Jang, Young Pyo, Van Den Hondel, Cees A M J J, Verpoorte, Robert, Klinkhamer, Peter G L, Choi, Young Hae, "Latex Metabolome of Euphorbia Species: Geographical and Inter-Species Variation and its Proposed Role in Plant Defense against Herbivores and Pathogens" in Journal of Chemical Ecology (2021),
https://doi.org/10.1007/s10886-021-01274-x . .

TY  - JOUR
AU  - Ge, Yanhui
AU  - Chen, Xiaojia
AU  - Gođevac, Dejan
AU  - Bueno, Paula C. P.
AU  - Salomé Abarca, Luis F.
AU  - Jang, Young Pyo
AU  - Wang, Mei
AU  - Choi, Young Hae
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3235
AB  - Ideally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling 1H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by 1H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.
PB  - Georg Thieme Verlag KG
T2  - Planta Medica
T1  - Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms
VL  - 85
IS  - 11/12
SP  - 917
EP  - 924
DO  - 10.1055/a-0947-5797
ER  - 

@article{
author = "Ge, Yanhui and Chen, Xiaojia and Gođevac, Dejan and Bueno, Paula C. P. and Salomé Abarca, Luis F. and Jang, Young Pyo and Wang, Mei and Choi, Young Hae",
year = "2019",
abstract = "Ideally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling 1H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by 1H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.",
publisher = "Georg Thieme Verlag KG",
journal = "Planta Medica",
title = "Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms",
volume = "85",
number = "11/12",
pages = "917-924",
doi = "10.1055/a-0947-5797"
}

Ge, Y., Chen, X., Gođevac, D., Bueno, P. C. P., Salomé Abarca, L. F., Jang, Y. P., Wang, M.,& Choi, Y. H.. (2019). Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms. in Planta Medica
Georg Thieme Verlag KG., 85(11/12), 917-924.
https://doi.org/10.1055/a-0947-5797

Ge Y, Chen X, Gođevac D, Bueno PCP, Salomé Abarca LF, Jang YP, Wang M, Choi YH. Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms. in Planta Medica. 2019;85(11/12):917-924.
doi:10.1055/a-0947-5797 .

Ge, Yanhui, Chen, Xiaojia, Gođevac, Dejan, Bueno, Paula C. P., Salomé Abarca, Luis F., Jang, Young Pyo, Wang, Mei, Choi, Young Hae, "Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms" in Planta Medica, 85, no. 11/12 (2019):917-924,
https://doi.org/10.1055/a-0947-5797 . .

TY  - JOUR
AU  - Krstić, Gordana
AU  - Anđelković, Boban D.
AU  - Choi, Young Hae
AU  - Vajs, Vlatka
AU  - Stevic, Tatjana
AU  - Tešević, Vele
AU  - Gođevac, Dejan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1936
AB  - The variations of metabolic profile of the latex of wild-growing Euphorbia palustris was carried out using multivariate analysis of NMR spectral data. One population was infected with fungi Fusarium sporotrichioides, Fusarium proliferatum and Alternaria alternata, while the other consisted of healthy plant species. The non-polar metabolites of latex extracts such as benzoyl ingenol-laurate, amyrin decadienoate esters, cis-1,4-polyisoprene, and 24-methylenecycloartanol were identified using H-1 and 2D NMR spectra. Principal component analysis of H-1 NMR data provided a clear discrimination between the latex of infected and healthy plants. Minimum inhibitory concentration and minimum fungicidal concentration values of the latex extracts of healthy and infected plants were determined. The latex of infected plants was found to contain higher levels of benzoyl ingenol-laurate and 24-methylenecycloartanol, of which concentrations were strongly correlated with the antifungal activities of the latex.
PB  - Oxford : Pergamon-Elsevier Science Ltd
T2  - Phytochemistry
T1  - Metabolic changes in Euphorbia palusrtis latex after fungal infection
VL  - 131
SP  - 17
EP  - 25
DO  - 10.1016/j.phytochem.2016.08.005
ER  - 

@article{
author = "Krstić, Gordana and Anđelković, Boban D. and Choi, Young Hae and Vajs, Vlatka and Stevic, Tatjana and Tešević, Vele and Gođevac, Dejan",
year = "2016",
abstract = "The variations of metabolic profile of the latex of wild-growing Euphorbia palustris was carried out using multivariate analysis of NMR spectral data. One population was infected with fungi Fusarium sporotrichioides, Fusarium proliferatum and Alternaria alternata, while the other consisted of healthy plant species. The non-polar metabolites of latex extracts such as benzoyl ingenol-laurate, amyrin decadienoate esters, cis-1,4-polyisoprene, and 24-methylenecycloartanol were identified using H-1 and 2D NMR spectra. Principal component analysis of H-1 NMR data provided a clear discrimination between the latex of infected and healthy plants. Minimum inhibitory concentration and minimum fungicidal concentration values of the latex extracts of healthy and infected plants were determined. The latex of infected plants was found to contain higher levels of benzoyl ingenol-laurate and 24-methylenecycloartanol, of which concentrations were strongly correlated with the antifungal activities of the latex.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "Phytochemistry",
title = "Metabolic changes in Euphorbia palusrtis latex after fungal infection",
volume = "131",
pages = "17-25",
doi = "10.1016/j.phytochem.2016.08.005"
}

Krstić, G., Anđelković, B. D., Choi, Y. H., Vajs, V., Stevic, T., Tešević, V.,& Gođevac, D.. (2016). Metabolic changes in Euphorbia palusrtis latex after fungal infection. in Phytochemistry
Oxford : Pergamon-Elsevier Science Ltd., 131, 17-25.
https://doi.org/10.1016/j.phytochem.2016.08.005

Krstić G, Anđelković BD, Choi YH, Vajs V, Stevic T, Tešević V, Gođevac D. Metabolic changes in Euphorbia palusrtis latex after fungal infection. in Phytochemistry. 2016;131:17-25.
doi:10.1016/j.phytochem.2016.08.005 .

Krstić, Gordana, Anđelković, Boban D., Choi, Young Hae, Vajs, Vlatka, Stevic, Tatjana, Tešević, Vele, Gođevac, Dejan, "Metabolic changes in Euphorbia palusrtis latex after fungal infection" in Phytochemistry, 131 (2016):17-25,
https://doi.org/10.1016/j.phytochem.2016.08.005 . .