TY  - JOUR
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Oreščanin-Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Stević, Zorica
PY  - 2020
UR  - https://www.eurekaselect.com/179582/article
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3966
AB  - Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.
PB  - Bentham Science
T2  - Current Drug Targets
T1  - Edaravone May Prevent Ferroptosis in ALS
VL  - 21
IS  - 8
SP  - 776
EP  - 780
DO  - 10.2174/1389450121666200220123305
ER  - 

@article{
author = "Spasić, Snežana and Nikolić-Kokić, Aleksandra and Miletić, Srđan and Oreščanin-Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško and Stević, Zorica",
year = "2020",
abstract = "Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.",
publisher = "Bentham Science",
journal = "Current Drug Targets",
title = "Edaravone May Prevent Ferroptosis in ALS",
volume = "21",
number = "8",
pages = "776-780",
doi = "10.2174/1389450121666200220123305"
}

Spasić, S., Nikolić-Kokić, A., Miletić, S., Oreščanin-Dušić, Z., Spasić, M., Blagojević, D.,& Stević, Z.. (2020). Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets
Bentham Science., 21(8), 776-780.
https://doi.org/10.2174/1389450121666200220123305

Spasić S, Nikolić-Kokić A, Miletić S, Oreščanin-Dušić Z, Spasić M, Blagojević D, Stević Z. Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets. 2020;21(8):776-780.
doi:10.2174/1389450121666200220123305 .

Spasić, Snežana, Nikolić-Kokić, Aleksandra, Miletić, Srđan, Oreščanin-Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, Stević, Zorica, "Edaravone May Prevent Ferroptosis in ALS" in Current Drug Targets, 21, no. 8 (2020):776-780,
https://doi.org/10.2174/1389450121666200220123305 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojevic, Dusko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2093
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
VL  - 100
SP  - 132
EP  - 136
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasić, Snežana and Miletić, Srđan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojevic, Dusko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
volume = "100",
pages = "132-136",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasić, S., Miletić, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojevic, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasić S, Miletić S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojevic D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasić, Snežana, Miletić, Srđan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojevic, Dusko, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojevic, Dusko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3043
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
VL  - 100
SP  - 132
EP  - 136
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasić, Snežana and Miletić, Srđan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojevic, Dusko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
volume = "100",
pages = "132-136",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasić, S., Miletić, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojevic, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasić S, Miletić S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojevic D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasić, Snežana, Miletić, Srđan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojevic, Dusko, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1787
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3187
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Bajic, Aleksandar
AU  - Zakrzewska, Joanna
AU  - Gođevac, Dejan
AU  - Anđus, Pavle
AU  - Jones, David R.
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/781
AB  - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
VL  - 346
IS  - 3
SP  - 416
EP  - 420
DO  - 10.1016/j.carres.2010.12.008
ER  - 

@article{
author = "Bajic, Aleksandar and Zakrzewska, Joanna and Gođevac, Dejan and Anđus, Pavle and Jones, David R. and Spasić, Mihajlo and Spasojević, Ivan",
year = "2011",
abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions",
volume = "346",
number = "3",
pages = "416-420",
doi = "10.1016/j.carres.2010.12.008"
}

Bajic, A., Zakrzewska, J., Gođevac, D., Anđus, P., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 346(3), 416-420.
https://doi.org/10.1016/j.carres.2010.12.008

Bajic A, Zakrzewska J, Gođevac D, Anđus P, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420.
doi:10.1016/j.carres.2010.12.008 .

Bajic, Aleksandar, Zakrzewska, Joanna, Gođevac, Dejan, Anđus, Pavle, Jones, David R., Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420,
https://doi.org/10.1016/j.carres.2010.12.008 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica
AU  - Spasić, Snežana
AU  - Jones, David R.
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/738
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Taylor & Francis Group
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
VL  - 15
IS  - 1
SP  - 29
EP  - 35
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica and Spasić, Snežana and Jones, David R. and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Taylor & Francis Group",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
volume = "15",
number = "1",
pages = "29-35",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z., Spasić, S., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Taylor & Francis Group., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević Z, Spasić S, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica, Spasić, Snežana, Jones, David R., Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3937
AB  - The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
VL  - 344
IS  - 1
SP  - 80
EP  - 84
DO  - 10.1016/j.carres.2008.09.025
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasić, Snežana and Jones, David and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
volume = "344",
number = "1",
pages = "80-84",
doi = "10.1016/j.carres.2008.09.025"
}

Spasojević, I., Mojović, M., Blagojević, D., Spasić, S., Jones, D., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025

Spasojević I, Mojović M, Blagojević D, Spasić S, Jones D, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .

Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasić, Snežana, Jones, David, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

TY  - JOUR
AU  - Filipović, Miloš R.
AU  - Stanić-Vučinić, Dragana
AU  - Raičević, Smiljana
AU  - Spasić, Mihajlo B.
AU  - Niketić, Vesna
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4335
AB  - The present study demonstrates that manganese superoxide dismutase (MnSOD) Escherichia coli, binds nitric oxide (NO) and stimulates its decay under both anaerobic and aerobic conditions. The results indicate that previously observed MnSOD-catalyzed NO disproportionation (dismutation) into nitrosonium (NO + ) and nitroxyl (NO - ) species under anaerobic conditions is also operative in the presence of molecular oxygen. Upon sustained aerobic exposure to NO, MnSOD-derived NO - species initiate the formation of peroxynitrite (ONOO - ) leading to enzyme tyrosine nitration, oxidation and (partial) inactivation. The results suggest that both ONOO - decomposition and ONOO - -dependent tyrosine residue nitration and oxidation are enhanced by metal centre-mediated catalysis. We show that the generation of ONOO - is accompanied by the formation of substantial amounts of H 2 O 2 . MnSOD is a critical mitochondrial antioxidant enzyme, which has been found to undergo tyrosine nitration and inactivation in various pathologies associated with the overproduction of NO. The results of the present study can account for the molecular specificity of MnSOD nitration in vivo. The interaction of NO with MnSOD may represent a novel mechanism by which MnSOD protects the cell from deleterious effects associated with overproduction of NO.
PB  - USA :Taylor & Francis INC
T2  - Free Radical Research
T1  - Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide
VL  - 41
IS  - 1
SP  - 62
EP  - 72
DO  - 10.1080/10715760600944296
ER  - 

@article{
author = "Filipović, Miloš R. and Stanić-Vučinić, Dragana and Raičević, Smiljana and Spasić, Mihajlo B. and Niketić, Vesna",
year = "2007",
abstract = "The present study demonstrates that manganese superoxide dismutase (MnSOD) Escherichia coli, binds nitric oxide (NO) and stimulates its decay under both anaerobic and aerobic conditions. The results indicate that previously observed MnSOD-catalyzed NO disproportionation (dismutation) into nitrosonium (NO + ) and nitroxyl (NO - ) species under anaerobic conditions is also operative in the presence of molecular oxygen. Upon sustained aerobic exposure to NO, MnSOD-derived NO - species initiate the formation of peroxynitrite (ONOO - ) leading to enzyme tyrosine nitration, oxidation and (partial) inactivation. The results suggest that both ONOO - decomposition and ONOO - -dependent tyrosine residue nitration and oxidation are enhanced by metal centre-mediated catalysis. We show that the generation of ONOO - is accompanied by the formation of substantial amounts of H 2 O 2 . MnSOD is a critical mitochondrial antioxidant enzyme, which has been found to undergo tyrosine nitration and inactivation in various pathologies associated with the overproduction of NO. The results of the present study can account for the molecular specificity of MnSOD nitration in vivo. The interaction of NO with MnSOD may represent a novel mechanism by which MnSOD protects the cell from deleterious effects associated with overproduction of NO.",
publisher = "USA :Taylor & Francis INC",
journal = "Free Radical Research",
title = "Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide",
volume = "41",
number = "1",
pages = "62-72",
doi = "10.1080/10715760600944296"
}

Filipović, M. R., Stanić-Vučinić, D., Raičević, S., Spasić, M. B.,& Niketić, V.. (2007). Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide. in Free Radical Research
USA :Taylor & Francis INC., 41(1), 62-72.
https://doi.org/10.1080/10715760600944296

Filipović MR, Stanić-Vučinić D, Raičević S, Spasić MB, Niketić V. Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide. in Free Radical Research. 2007;41(1):62-72.
doi:10.1080/10715760600944296 .

Filipović, Miloš R., Stanić-Vučinić, Dragana, Raičević, Smiljana, Spasić, Mihajlo B., Niketić, Vesna, "Consequences of MnSOD interactions with nitric oxide: Nitric oxide dismutation and the generation of peroxynitrite and hydrogen peroxide" in Free Radical Research, 41, no. 1 (2007):62-72,
https://doi.org/10.1080/10715760600944296 . .

TY  - JOUR
AU  - Oreščanin, Zorana
AU  - Milovanović, Slobodan R.
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Spasić, Mihajlo
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3938
AB  - The conversion of nitric oxide (NO
) into its congeners nitrosonium (NO ) and nitroxyl (HNO/NO) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert
NO into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and
presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced
relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO-mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not
solely endothelium-derived but involves changes in vascular smooth muscl
PB  - Springer
T2  - Pharmacological Report
T1  - Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners
VL  - 59
SP  - 315
EP  - 322
UR  - https://hdl.handle.net/21.15107/rcub_cer_3938
ER  - 

@article{
author = "Oreščanin, Zorana and Milovanović, Slobodan R. and Spasić, Snežana and Jones, David and Spasić, Mihajlo",
year = "2007",
abstract = "The conversion of nitric oxide (NO
) into its congeners nitrosonium (NO ) and nitroxyl (HNO/NO) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert
NO into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and
presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced
relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO-mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not
solely endothelium-derived but involves changes in vascular smooth muscl",
publisher = "Springer",
journal = "Pharmacological Report",
title = "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners",
volume = "59",
pages = "315-322",
url = "https://hdl.handle.net/21.15107/rcub_cer_3938"
}

Oreščanin, Z., Milovanović, S. R., Spasić, S., Jones, D.,& Spasić, M.. (2007). Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Report
Springer., 59, 315-322.
https://hdl.handle.net/21.15107/rcub_cer_3938

Oreščanin Z, Milovanović SR, Spasić S, Jones D, Spasić M. Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Report. 2007;59:315-322.
https://hdl.handle.net/21.15107/rcub_cer_3938 .

Oreščanin, Zorana, Milovanović, Slobodan R., Spasić, Snežana, Jones, David, Spasić, Mihajlo, "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners" in Pharmacological Report, 59 (2007):315-322,
https://hdl.handle.net/21.15107/rcub_cer_3938 .

TY  - JOUR
AU  - Nikolić, Milan
AU  - Nikolić -Kokić, Aleksandra
AU  - Stanić, Dragana
AU  - Blagojević, Duško
AU  - Vranić, Danijela
AU  - Jones, David R.
AU  - Niketić, Vesna
AU  - Spasić, Mihajlo B.
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4219
AB  - U prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu
zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin
(Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski
sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze,
glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb)
u eritrocitima 60 qudi, podeqenih u dve grupe na osnovu količine Hb-Ch. Rezultati
pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo
metHb.Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne
promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom.
U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida
u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi
rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima
prilagođava svoju organizaciju prema zahtevima iz svog okruženja.
AB  - In a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?
T1  - Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima
VL  - 72
IS  - 4
SP  - 339
EP  - 345
DO  - 10.2298/JSC0704339N
ER  - 

@article{
author = "Nikolić, Milan and Nikolić -Kokić, Aleksandra and Stanić, Dragana and Blagojević, Duško and Vranić, Danijela and Jones, David R. and Niketić, Vesna and Spasić, Mihajlo B.",
year = "2007",
abstract = "U prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu
zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin
(Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski
sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze,
glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb)
u eritrocitima 60 qudi, podeqenih u dve grupe na osnovu količine Hb-Ch. Rezultati
pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo
metHb.Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne
promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom.
U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida
u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi
rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima
prilagođava svoju organizaciju prema zahtevima iz svog okruženja., In a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?, Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima",
volume = "72",
number = "4",
pages = "339-345",
doi = "10.2298/JSC0704339N"
}

Nikolić, M., Nikolić -Kokić, A., Stanić, D., Blagojević, D., Vranić, D., Jones, D. R., Niketić, V.,& Spasić, M. B.. (2007). Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society., 72(4), 339-345.
https://doi.org/10.2298/JSC0704339N

Nikolić M, Nikolić -Kokić A, Stanić D, Blagojević D, Vranić D, Jones DR, Niketić V, Spasić MB. Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?. in Journal of the Serbian Chemical Society. 2007;72(4):339-345.
doi:10.2298/JSC0704339N .

Nikolić, Milan, Nikolić -Kokić, Aleksandra, Stanić, Dragana, Blagojević, Duško, Vranić, Danijela, Jones, David R., Niketić, Vesna, Spasić, Mihajlo B., "Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?" in Journal of the Serbian Chemical Society, 72, no. 4 (2007):339-345,
https://doi.org/10.2298/JSC0704339N . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vucić, Vesna
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Radojčić, Marija
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3965
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappaB and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappaB and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
PB  - Maney Publishing
T2  - Redox Report
T1  - Systemic NF-kappaB activation in blood cells of breast cancer patients
VL  - 11
IS  - 1
SP  - 39
SP  - 44
DO  - 10.1179/135100006X101002
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Vucić, Vesna and Nešković-Konstantinović, Zora and Spasić, Snežana and Jones, David and Radojčić, Marija and Spasić, Mihajlo",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappaB and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappaB and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
publisher = "Maney Publishing",
journal = "Redox Report",
title = "Systemic NF-kappaB activation in blood cells of breast cancer patients",
volume = "11",
number = "1",
pages = "39-44",
doi = "10.1179/135100006X101002"
}

Adžić, M., Nićiforović, A., Vucić, V., Nešković-Konstantinović, Z., Spasić, S., Jones, D., Radojčić, M.,& Spasić, M.. (2006). Systemic NF-kappaB activation in blood cells of breast cancer patients. in Redox Report
Maney Publishing., 11(1), 39.
https://doi.org/10.1179/135100006X101002

Adžić M, Nićiforović A, Vucić V, Nešković-Konstantinović Z, Spasić S, Jones D, Radojčić M, Spasić M. Systemic NF-kappaB activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):39.
doi:10.1179/135100006X101002 .

Adžić, Miroslav, Nićiforović, Ana, Vucić, Vesna, Nešković-Konstantinović, Zora, Spasić, Snežana, Jones, David, Radojčić, Marija, Spasić, Mihajlo, "Systemic NF-kappaB activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006):39,
https://doi.org/10.1179/135100006X101002 . .

TY  - CONF
AU  - Niketić, Vesna
AU  - Stojanović, Srđan
AU  - Stanić, Dragana
AU  - Nikolić, Milan
AU  - Raičević, Smiljana
AU  - Spasić, Mihajlo
PY  - 2005
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6512
AB  - Rezultati određivanja nitrita, S-nitrozotiola (RSNO) i hidroksilamina (karakterističnih proizvoda NO+
odnosno NO- vrsta) u rastvorima MnSOD (E. coli) i niskomolekulskih tiola tretiranih azot-monoksidom, pod
striktno anaerobnim uslovima, ubedljivo pokazuju da ovaj enzim katalizuje dismutaciju NO u NO+ i NO- vrste.
Generisane RNOS izazivaju modifikacije (uključujući i nitrovanje ostatka tirozina) molekula enzima, što ima za
posledicu inaktivaciju enzima. Modifikacija i inaktivacija MnSOD nastala kao posledica NO dismutacije mnogo
je manja i sporija u odnosu na one izazvane peroksinitritom.
Polazeći od sličnosti u strukturi MnSOD (E. coli) i humane MnSOD pretpostavljeno je da i humana
MnSOD katalizuje NO dismutaciju, te da ostatak cisteina u blizini aktivnog centra molekula enzima reaguje
primarno sa generisanim RNOS, usporavajući tako nitrovanje ostatka tirozina u aktivnom mestu, a time i
inaktivaciju enzima. Ponašanje MnSOD kao NO dismutaze imalo bi protektivnu ulogu u uslovima prekomerne
produkcije NO, jer bi sprečavalo njegovu transformaciju u štetne RNOS.
PB  - Belgrade : Serbian Chemical Society
C3  - Kratki izvodi radova - 43. Savetovanje Srpskog hemijskog društva, 24. i 25. januar 2005, Beograd
T1  - Da li bi mangan superoksid dismutaza (MnSOD) mogla da ima ulogu NO dismutaze?
T1  - Could manganese superoxide dismutase (MnSOD) play a role as NO dismutase?
SP  - 117
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_cer_6512
ER  - 

@conference{
author = "Niketić, Vesna and Stojanović, Srđan and Stanić, Dragana and Nikolić, Milan and Raičević, Smiljana and Spasić, Mihajlo",
year = "2005",
abstract = "Rezultati određivanja nitrita, S-nitrozotiola (RSNO) i hidroksilamina (karakterističnih proizvoda NO+
odnosno NO- vrsta) u rastvorima MnSOD (E. coli) i niskomolekulskih tiola tretiranih azot-monoksidom, pod
striktno anaerobnim uslovima, ubedljivo pokazuju da ovaj enzim katalizuje dismutaciju NO u NO+ i NO- vrste.
Generisane RNOS izazivaju modifikacije (uključujući i nitrovanje ostatka tirozina) molekula enzima, što ima za
posledicu inaktivaciju enzima. Modifikacija i inaktivacija MnSOD nastala kao posledica NO dismutacije mnogo
je manja i sporija u odnosu na one izazvane peroksinitritom.
Polazeći od sličnosti u strukturi MnSOD (E. coli) i humane MnSOD pretpostavljeno je da i humana
MnSOD katalizuje NO dismutaciju, te da ostatak cisteina u blizini aktivnog centra molekula enzima reaguje
primarno sa generisanim RNOS, usporavajući tako nitrovanje ostatka tirozina u aktivnom mestu, a time i
inaktivaciju enzima. Ponašanje MnSOD kao NO dismutaze imalo bi protektivnu ulogu u uslovima prekomerne
produkcije NO, jer bi sprečavalo njegovu transformaciju u štetne RNOS.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Kratki izvodi radova - 43. Savetovanje Srpskog hemijskog društva, 24. i 25. januar 2005, Beograd",
title = "Da li bi mangan superoksid dismutaza (MnSOD) mogla da ima ulogu NO dismutaze?, Could manganese superoxide dismutase (MnSOD) play a role as NO dismutase?",
pages = "117-120",
url = "https://hdl.handle.net/21.15107/rcub_cer_6512"
}

Niketić, V., Stojanović, S., Stanić, D., Nikolić, M., Raičević, S.,& Spasić, M.. (2005). Da li bi mangan superoksid dismutaza (MnSOD) mogla da ima ulogu NO dismutaze?. in Kratki izvodi radova - 43. Savetovanje Srpskog hemijskog društva, 24. i 25. januar 2005, Beograd
Belgrade : Serbian Chemical Society., 117-120.
https://hdl.handle.net/21.15107/rcub_cer_6512

Niketić V, Stojanović S, Stanić D, Nikolić M, Raičević S, Spasić M. Da li bi mangan superoksid dismutaza (MnSOD) mogla da ima ulogu NO dismutaze?. in Kratki izvodi radova - 43. Savetovanje Srpskog hemijskog društva, 24. i 25. januar 2005, Beograd. 2005;:117-120.
https://hdl.handle.net/21.15107/rcub_cer_6512 .

Niketić, Vesna, Stojanović, Srđan, Stanić, Dragana, Nikolić, Milan, Raičević, Smiljana, Spasić, Mihajlo, "Da li bi mangan superoksid dismutaza (MnSOD) mogla da ima ulogu NO dismutaze?" in Kratki izvodi radova - 43. Savetovanje Srpskog hemijskog društva, 24. i 25. januar 2005, Beograd (2005):117-120,
https://hdl.handle.net/21.15107/rcub_cer_6512 .

TY  - JOUR
AU  - Stojanović, Srđan
AU  - Stanić, Dragana
AU  - Nikolić, Milan
AU  - Raičević, Smiljana
AU  - Spasić, Mihajlo B.
AU  - Niketić, Vesna
PY  - 2005
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/215
AB  - The peroxynitrite-induced nitration of manganese superoxide dismutase (MnSOD) tyrosine residue, which causes enzyme inactivation, is well established. This led to suggestions that MnSOD nitration and inactivation in vivo, detected in various diseases associated with oxidative stress and overproduction of nitric monoxide (NO), conditions which favor peroxynitrite formation, is also caused by peroxynitrite. However, our previous in vitro study demonstrated that exposure of MnSOD to NO led to NO conversion into nitrosonium (NO+) and nitroxyl (NO–) species, which caused enzyme modifications and inactivation. Here it is reported that MnSOD is tyrosine nitrated upon exposure to NO, as well as that MnSOD nitration contributes to inactivation of the enzyme. Collectively, these observations provide a compelling argument supporting the generation of nitrating species in MnSOD exposed to NO and shed a new light on MnSOD tyrosine nitration and inactivation in vivo. This may represent a novel mechanism by which MnSOD protects cell from deleterious effects associated with overproduction of NO. However, extensive MnSOD modification and inactivation associated with prolonged exposure to NO will amplify the toxic effects caused by increased cell superoxide and NO levels.
AB  - Dobro je poznato da peroksinitrit izaziva nitrovanje ostataka tirozina u mangan-superoksid- dismutazi (MnSOD) što dovodi do inaktivacije enzima. Pokazano je da nitrovanje i inaktivacija MnSOD-a nastaje u raznim bolestima za koje je karakteristič an oksidativni stres i povećana produkcija azot-monoksida (NO). Pošto se pri ovim uslovima očekuje nastajanje peroksinitrita predloženo je da peroksinitrit izaziva nitrovanje i inaktivaciju MnSOD in vivo. U našem prethodnom radu pokazali smo da MnSOD katalizuje transformaciju NO u nitrozonijum (NO+) i nitroksil (NO–) reaktivne vrste, te identifikovali neke od modifikacija molekula enzima koje pri tome nastaju izazivajući njegovu inaktivaciju. U ovom radu je pokazano da pri izlaganju MnSOD azot-monoksidu dolazi i do nitrovanja ostatka tirozina u molekulu enzima, što doprinosi njegovoj inaktivaciji. Ovi rezultati ukazuju da pri interakciji MnSOD sa NO dolazi do nastajanja nitrujućih vrsta, što baca novo svetlo na proces nitrovanja ostataka tirozina i inaktivaciju MnSOD in vivo. Ovo može da predstavlja novi mehanizam kojim MnSOD štiti ćeliju odštetnih efekata izazvanih hiperprodukcijom azot-monoksida. Međutim ekstenzivne modifikacije i inaktivacija MnSOD do kojih dolazi pri produženom izlaganju enzima NO, uvećaće toksične efekte izazvane povećanim koncentracijama superoksida i NO u ćeliji.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration
T1  - Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina
VL  - 70
IS  - 4
SP  - 601
EP  - 608
DO  - 10.2298/JSC0504601S
ER  - 

@article{
author = "Stojanović, Srđan and Stanić, Dragana and Nikolić, Milan and Raičević, Smiljana and Spasić, Mihajlo B. and Niketić, Vesna",
year = "2005",
abstract = "The peroxynitrite-induced nitration of manganese superoxide dismutase (MnSOD) tyrosine residue, which causes enzyme inactivation, is well established. This led to suggestions that MnSOD nitration and inactivation in vivo, detected in various diseases associated with oxidative stress and overproduction of nitric monoxide (NO), conditions which favor peroxynitrite formation, is also caused by peroxynitrite. However, our previous in vitro study demonstrated that exposure of MnSOD to NO led to NO conversion into nitrosonium (NO+) and nitroxyl (NO–) species, which caused enzyme modifications and inactivation. Here it is reported that MnSOD is tyrosine nitrated upon exposure to NO, as well as that MnSOD nitration contributes to inactivation of the enzyme. Collectively, these observations provide a compelling argument supporting the generation of nitrating species in MnSOD exposed to NO and shed a new light on MnSOD tyrosine nitration and inactivation in vivo. This may represent a novel mechanism by which MnSOD protects cell from deleterious effects associated with overproduction of NO. However, extensive MnSOD modification and inactivation associated with prolonged exposure to NO will amplify the toxic effects caused by increased cell superoxide and NO levels., Dobro je poznato da peroksinitrit izaziva nitrovanje ostataka tirozina u mangan-superoksid- dismutazi (MnSOD) što dovodi do inaktivacije enzima. Pokazano je da nitrovanje i inaktivacija MnSOD-a nastaje u raznim bolestima za koje je karakteristič an oksidativni stres i povećana produkcija azot-monoksida (NO). Pošto se pri ovim uslovima očekuje nastajanje peroksinitrita predloženo je da peroksinitrit izaziva nitrovanje i inaktivaciju MnSOD in vivo. U našem prethodnom radu pokazali smo da MnSOD katalizuje transformaciju NO u nitrozonijum (NO+) i nitroksil (NO–) reaktivne vrste, te identifikovali neke od modifikacija molekula enzima koje pri tome nastaju izazivajući njegovu inaktivaciju. U ovom radu je pokazano da pri izlaganju MnSOD azot-monoksidu dolazi i do nitrovanja ostatka tirozina u molekulu enzima, što doprinosi njegovoj inaktivaciji. Ovi rezultati ukazuju da pri interakciji MnSOD sa NO dolazi do nastajanja nitrujućih vrsta, što baca novo svetlo na proces nitrovanja ostataka tirozina i inaktivaciju MnSOD in vivo. Ovo može da predstavlja novi mehanizam kojim MnSOD štiti ćeliju odštetnih efekata izazvanih hiperprodukcijom azot-monoksida. Međutim ekstenzivne modifikacije i inaktivacija MnSOD do kojih dolazi pri produženom izlaganju enzima NO, uvećaće toksične efekte izazvane povećanim koncentracijama superoksida i NO u ćeliji.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration, Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina",
volume = "70",
number = "4",
pages = "601-608",
doi = "10.2298/JSC0504601S"
}

Stojanović, S., Stanić, D., Nikolić, M., Raičević, S., Spasić, M. B.,& Niketić, V.. (2005). Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 70(4), 601-608.
https://doi.org/10.2298/JSC0504601S

Stojanović S, Stanić D, Nikolić M, Raičević S, Spasić MB, Niketić V. Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration. in Journal of the Serbian Chemical Society. 2005;70(4):601-608.
doi:10.2298/JSC0504601S .

Stojanović, Srđan, Stanić, Dragana, Nikolić, Milan, Raičević, Smiljana, Spasić, Mihajlo B., Niketić, Vesna, "Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration" in Journal of the Serbian Chemical Society, 70, no. 4 (2005):601-608,
https://doi.org/10.2298/JSC0504601S . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Stević, Zorica
AU  - Stojanović, Srđan
AU  - Blagojević, Duško
AU  - Jones, David
AU  - Pavlović, Sanja
AU  - Nikrtić, Vesna
AU  - Apostolski, Slobodan
AU  - Spasić, Mihajlo
PY  - 2005
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3868
AB  - Recent findings indicate that nitric oxide (NO•) over-production might be an important factor in thepathogenesis  of  sporadic  amyotrophic  lateral  sclerosis  (SALS).  We  measured  significantly  higherconcentrations   of   uric   acid   and   thiol   group-containing   molecules   (R–SH   groups)   in   thecerebrospinal  fluid  (CSF)  from  SALS  patients  compared  to  controls.  The  above  factors,  togetherwith a slightly increased free iron concentration found in the CSF, favour conditions necessary forthe  formation  of  the  dinitrosyl  iron  complex,  capable  of  NO•bio-transformation.  Thus,  weperformed ex vivosaturation of CSF (from both SALS patients and controls) with NO•. A decreasein the level of R–SH was found. This was more pronounced in the CSF from SALS patients. In theCSF from SALS patients the production of nitrite and hydroxylamine was greater than that observedin the CSF from controls. Moreover, we also found increased Cu,Zn-SOD activity in the CSF fromSALS  patients  (when  compared  to  control  subjects)  but  no  activity  corresponding  to  Mn-SOD  inany CSF samples. As Cu,Zn-SOD can react with nitroxyl forming NO•, the conditions for a closed,but continuous, loop of NO•biotransformation are present in the CSF of ALS patients.
PB  - Maney Publishing
T2  - Redox Report
T1  - Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
VL  - 10
IS  - 5
SP  - 265
EP  - 270
DO  - 10.1179/135100005X70242
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Stević, Zorica and Stojanović, Srđan and Blagojević, Duško and Jones, David and Pavlović, Sanja and Nikrtić, Vesna and Apostolski, Slobodan and Spasić, Mihajlo",
year = "2005",
abstract = "Recent findings indicate that nitric oxide (NO•) over-production might be an important factor in thepathogenesis  of  sporadic  amyotrophic  lateral  sclerosis  (SALS).  We  measured  significantly  higherconcentrations   of   uric   acid   and   thiol   group-containing   molecules   (R–SH   groups)   in   thecerebrospinal  fluid  (CSF)  from  SALS  patients  compared  to  controls.  The  above  factors,  togetherwith a slightly increased free iron concentration found in the CSF, favour conditions necessary forthe  formation  of  the  dinitrosyl  iron  complex,  capable  of  NO•bio-transformation.  Thus,  weperformed ex vivosaturation of CSF (from both SALS patients and controls) with NO•. A decreasein the level of R–SH was found. This was more pronounced in the CSF from SALS patients. In theCSF from SALS patients the production of nitrite and hydroxylamine was greater than that observedin the CSF from controls. Moreover, we also found increased Cu,Zn-SOD activity in the CSF fromSALS  patients  (when  compared  to  control  subjects)  but  no  activity  corresponding  to  Mn-SOD  inany CSF samples. As Cu,Zn-SOD can react with nitroxyl forming NO•, the conditions for a closed,but continuous, loop of NO•biotransformation are present in the CSF of ALS patients.",
publisher = "Maney Publishing",
journal = "Redox Report",
title = "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
volume = "10",
number = "5",
pages = "265-270",
doi = "10.1179/135100005X70242"
}

Nikolić-Kokić, A., Stević, Z., Stojanović, S., Blagojević, D., Jones, D., Pavlović, S., Nikrtić, V., Apostolski, S.,& Spasić, M.. (2005). Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report
Maney Publishing., 10(5), 265-270.
https://doi.org/10.1179/135100005X70242

Nikolić-Kokić A, Stević Z, Stojanović S, Blagojević D, Jones D, Pavlović S, Nikrtić V, Apostolski S, Spasić M. Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2005;10(5):265-270.
doi:10.1179/135100005X70242 .

Nikolić-Kokić, Aleksandra, Stević, Zorica, Stojanović, Srđan, Blagojević, Duško, Jones, David, Pavlović, Sanja, Nikrtić, Vesna, Apostolski, Slobodan, Spasić, Mihajlo, "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 10, no. 5 (2005):265-270,
https://doi.org/10.1179/135100005X70242 . .

TY  - JOUR
AU  - Milovanović, Slobodan R.
AU  - Oreščanin, Zorana
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Prostran, Milica
AU  - Spasić, Mihajlo B.
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/141
AB  - In this study themolecular foundation of nitric oxide induced relaxation of arteries, with or without endothelium, of normotensive and spontanously hypertensive ratswas re-examined. With this purpose in mind, the effects of the nitric oxide donor sodium nitroprusside (NaNP), with and without manganese containing superoxide dismutase (MnSOD E.C. 1.15.1.1), on rat renal artery relaxation was strudied. The results show that the relaxation effect of NaNP is two times higher in normotensive, compared to spontaneously hypertensive rats. Similar differences exist in the relaxation effects of NaNP on isolated renal arteries without endothelium, indicating that besides the difference in the function of an endothelium, concerning basal NO production in normotensive and hypertensive rats, there is a differencewith respect to NO relaxation in the smoothmuscle that is induced by hypertension. MnSOD decreased the relaxation effect of NaNP in all the examined renal arteries, more in normotensive than in hypertensive ones regardless of the presence of an endothelium. These results show that MnSOD, by modifying the chemical versatility of NO into redox active forms - nitrosonium (NO+) and nitroxyl (NO-), produces different relaxation effects in normotensive and hypertensive arteries of rats, with or without an endothelium, potentiating the role of nitroxyl induced relaxation in sponteneously hypertensive rats. The results prove the need for the synthesis of complex NO donors, as the mechanisms of artery relaxation are different due to an endothel and smooth mouscle changes in hypertensive, as compared to normotensive rats.
AB  - U ovom radu smo pokušali da detaljnije ispitamo molekulsku osnovu azotoksid indukovane relaksacije arterija, sa i bez endotela, normotenzivnih i spontano hipertenzivnih pacova. U tu svrhu ispitivan je efekat azot-oksid (NO) donora natrijum- nitroprusida (NaNP), bez i u prisustvu superoksid-dismutaze koja sadrži mangan (MnSOD, EC 1.15.1.1) na relaksaciju renalne arterije. Rezulati pokazuju dvostruko veći relaksantni efekat NaNP kod normotenzivnih, u odnosu na spontano hipertenzivne pacove. Slična razlika postoji i u relaksantnom efektu NaNP na izolovane renalne arterije normotenzivnog i hipertenzivnog pacova kada je odstranjen endotel što ukazuje da, pored razlike u funciji endotela u odnosu na bazalnu NO produkciju kod normotenzivnih i spontano hipertenzivnih pacova, postoje i promene u glatkim mišićima indukovane hipertenzijom, u odnosu na NO relaksaciju. MnSOD kod svih grupa smanjuje relaksantni efekat SNP i to više kod normotenzivnih nego kod hipertenzivnih nezavisno od prisustva endotela. Ovi rezultati pokazuju da MnSOD menjajući hemijsku prirodu iz NaNP oslobođenog NO u redoks aktivne forme – nitrozonijum (NO+) i nitroksil (NO–) – ostvaruje različit relaksantni efekat kod normotenzivnih i kod hiperetenzivnih pacova sa i bez endotela potencirajući značaj nitroksilom indukovane relaksacije kod spontano-hipertenzivnih pacova. Dobijeni rezultati potvrđuju potrebu sinteze NO donora koji daju različite redoks-aktivne forme NO budući da se primarni mehanismi relaksacije razlikuju u zavisnosti od karakteristika endotela i glatkih mišića, kod hipertenzije u odnosu na normotenzivno stanje.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery
T1  - Efekat MnSOD (E. coli) Na relaksaciju izolovane renalne arterije pacova izazvanu natrijum-nitro-prusidom
VL  - 69
IS  - 11
SP  - 973
EP  - 980
DO  - 10.2298/JSC0411973M
ER  - 

@article{
author = "Milovanović, Slobodan R. and Oreščanin, Zorana and Spasić, Snežana and Miletić, Srđan and Prostran, Milica and Spasić, Mihajlo B.",
year = "2004",
abstract = "In this study themolecular foundation of nitric oxide induced relaxation of arteries, with or without endothelium, of normotensive and spontanously hypertensive ratswas re-examined. With this purpose in mind, the effects of the nitric oxide donor sodium nitroprusside (NaNP), with and without manganese containing superoxide dismutase (MnSOD E.C. 1.15.1.1), on rat renal artery relaxation was strudied. The results show that the relaxation effect of NaNP is two times higher in normotensive, compared to spontaneously hypertensive rats. Similar differences exist in the relaxation effects of NaNP on isolated renal arteries without endothelium, indicating that besides the difference in the function of an endothelium, concerning basal NO production in normotensive and hypertensive rats, there is a differencewith respect to NO relaxation in the smoothmuscle that is induced by hypertension. MnSOD decreased the relaxation effect of NaNP in all the examined renal arteries, more in normotensive than in hypertensive ones regardless of the presence of an endothelium. These results show that MnSOD, by modifying the chemical versatility of NO into redox active forms - nitrosonium (NO+) and nitroxyl (NO-), produces different relaxation effects in normotensive and hypertensive arteries of rats, with or without an endothelium, potentiating the role of nitroxyl induced relaxation in sponteneously hypertensive rats. The results prove the need for the synthesis of complex NO donors, as the mechanisms of artery relaxation are different due to an endothel and smooth mouscle changes in hypertensive, as compared to normotensive rats., U ovom radu smo pokušali da detaljnije ispitamo molekulsku osnovu azotoksid indukovane relaksacije arterija, sa i bez endotela, normotenzivnih i spontano hipertenzivnih pacova. U tu svrhu ispitivan je efekat azot-oksid (NO) donora natrijum- nitroprusida (NaNP), bez i u prisustvu superoksid-dismutaze koja sadrži mangan (MnSOD, EC 1.15.1.1) na relaksaciju renalne arterije. Rezulati pokazuju dvostruko veći relaksantni efekat NaNP kod normotenzivnih, u odnosu na spontano hipertenzivne pacove. Slična razlika postoji i u relaksantnom efektu NaNP na izolovane renalne arterije normotenzivnog i hipertenzivnog pacova kada je odstranjen endotel što ukazuje da, pored razlike u funciji endotela u odnosu na bazalnu NO produkciju kod normotenzivnih i spontano hipertenzivnih pacova, postoje i promene u glatkim mišićima indukovane hipertenzijom, u odnosu na NO relaksaciju. MnSOD kod svih grupa smanjuje relaksantni efekat SNP i to više kod normotenzivnih nego kod hipertenzivnih nezavisno od prisustva endotela. Ovi rezultati pokazuju da MnSOD menjajući hemijsku prirodu iz NaNP oslobođenog NO u redoks aktivne forme – nitrozonijum (NO+) i nitroksil (NO–) – ostvaruje različit relaksantni efekat kod normotenzivnih i kod hiperetenzivnih pacova sa i bez endotela potencirajući značaj nitroksilom indukovane relaksacije kod spontano-hipertenzivnih pacova. Dobijeni rezultati potvrđuju potrebu sinteze NO donora koji daju različite redoks-aktivne forme NO budući da se primarni mehanismi relaksacije razlikuju u zavisnosti od karakteristika endotela i glatkih mišića, kod hipertenzije u odnosu na normotenzivno stanje.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery, Efekat MnSOD (E. coli) Na relaksaciju izolovane renalne arterije pacova izazvanu natrijum-nitro-prusidom",
volume = "69",
number = "11",
pages = "973-980",
doi = "10.2298/JSC0411973M"
}

Milovanović, S. R., Oreščanin, Z., Spasić, S., Miletić, S., Prostran, M.,& Spasić, M. B.. (2004). Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 69(11), 973-980.
https://doi.org/10.2298/JSC0411973M

Milovanović SR, Oreščanin Z, Spasić S, Miletić S, Prostran M, Spasić MB. Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery. in Journal of the Serbian Chemical Society. 2004;69(11):973-980.
doi:10.2298/JSC0411973M .

Milovanović, Slobodan R., Oreščanin, Zorana, Spasić, Snežana, Miletić, Srđan, Prostran, Milica, Spasić, Mihajlo B., "Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery" in Journal of the Serbian Chemical Society, 69, no. 11 (2004):973-980,
https://doi.org/10.2298/JSC0411973M . .

TY  - JOUR
AU  - Stojanović, Srđan
AU  - Stanić, Dragana
AU  - Nikolić, Milan
AU  - Spasić, Mihajlo
AU  - Niketić, Vesna
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2682
AB  - The conversion of NO into its congeners, nitrosonium (NO+) and nitroxyl (HNO/NO-) species, has important consequences in NO metabolism. Dinitrosyl iron complex (DNIC) combined with thiol ligands was shown to catalyze the conversion of NO into NO+, resulting in the synthesis of S-nitrosothiols (RSNO) both in vitro and in vivo. The formation mechanism of DNIC was proposed to involve the intermediate release of nitroxyl. Since the detection of hydroxylamine (as the product of a rapid reaction of HNO/NO- with thiols) is taken as the evidence for nitroxyl generation, we examined the formation of hydroxylamine, RSNO, and nitrite (the product of a rapid reaction of NO+ with water) in neutral solutions containing iron ions and thiols exposed to NO under anaerobic conditions. Hydroxylamine was detected in NO treated solutions of iron ions in the presence of cysteine, but not glutathione (GSH). The addition of urate, a major "free" iron-binding agent in humans, to solutions of GSH and iron ions, and the subsequent treatment of these solutions with NO increased the synthesis of GSNO and resulted in the formation of hydroxylamine. This caused a loss of urate and yielded a novel nitrosative/nitration product. GSH attenuated the urate decomposition to such a degree that it could be reflected as the function of GSH:urate. Results described here contribute to the understanding of the role of iron ions in catalyzing the conversion of NO into HNO/NO- and point to the role of uric acid not previously described. (C) 2004 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Nitric Oxide: Biology and Chemistry
T1  - Iron catalyzed conversion of NO into nitrosonium (NO+) and nitroxyl (HNO/NO-) species
VL  - 11
IS  - 3
SP  - 256
EP  - 262
DO  - 10.1016/j.niox.2004.09.007
ER  - 

@article{
author = "Stojanović, Srđan and Stanić, Dragana and Nikolić, Milan and Spasić, Mihajlo and Niketić, Vesna",
year = "2004",
abstract = "The conversion of NO into its congeners, nitrosonium (NO+) and nitroxyl (HNO/NO-) species, has important consequences in NO metabolism. Dinitrosyl iron complex (DNIC) combined with thiol ligands was shown to catalyze the conversion of NO into NO+, resulting in the synthesis of S-nitrosothiols (RSNO) both in vitro and in vivo. The formation mechanism of DNIC was proposed to involve the intermediate release of nitroxyl. Since the detection of hydroxylamine (as the product of a rapid reaction of HNO/NO- with thiols) is taken as the evidence for nitroxyl generation, we examined the formation of hydroxylamine, RSNO, and nitrite (the product of a rapid reaction of NO+ with water) in neutral solutions containing iron ions and thiols exposed to NO under anaerobic conditions. Hydroxylamine was detected in NO treated solutions of iron ions in the presence of cysteine, but not glutathione (GSH). The addition of urate, a major "free" iron-binding agent in humans, to solutions of GSH and iron ions, and the subsequent treatment of these solutions with NO increased the synthesis of GSNO and resulted in the formation of hydroxylamine. This caused a loss of urate and yielded a novel nitrosative/nitration product. GSH attenuated the urate decomposition to such a degree that it could be reflected as the function of GSH:urate. Results described here contribute to the understanding of the role of iron ions in catalyzing the conversion of NO into HNO/NO- and point to the role of uric acid not previously described. (C) 2004 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Nitric Oxide: Biology and Chemistry",
title = "Iron catalyzed conversion of NO into nitrosonium (NO+) and nitroxyl (HNO/NO-) species",
volume = "11",
number = "3",
pages = "256-262",
doi = "10.1016/j.niox.2004.09.007"
}

Stojanović, S., Stanić, D., Nikolić, M., Spasić, M.,& Niketić, V.. (2004). Iron catalyzed conversion of NO into nitrosonium (NO+) and nitroxyl (HNO/NO-) species. in Nitric Oxide: Biology and Chemistry
Elsevier., 11(3), 256-262.
https://doi.org/10.1016/j.niox.2004.09.007

Stojanović S, Stanić D, Nikolić M, Spasić M, Niketić V. Iron catalyzed conversion of NO into nitrosonium (NO+) and nitroxyl (HNO/NO-) species. in Nitric Oxide: Biology and Chemistry. 2004;11(3):256-262.
doi:10.1016/j.niox.2004.09.007 .

Stojanović, Srđan, Stanić, Dragana, Nikolić, Milan, Spasić, Mihajlo, Niketić, Vesna, "Iron catalyzed conversion of NO into nitrosonium (NO+) and nitroxyl (HNO/NO-) species" in Nitric Oxide: Biology and Chemistry, 11, no. 3 (2004):256-262,
https://doi.org/10.1016/j.niox.2004.09.007 . .

TY  - CONF
AU  - Niketić, Svetozar
AU  - Niketić, Vesna
AU  - Stojanović, Srđan
AU  - Spasić, Mihajlo
PY  - 1999
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6538
AB  - Ligand field analysis (in the AOM formalism) of Mn(ll, lll) in seven Mn SOD structures reported in the Brookhaven PDB show non-negligible differences in d-orbital energies. The results are equally affected by the azimuthal deformations of the coordination polyhedron, as well as by the orientations of the His imidazole rings with respect to the Mn-N bonds or of the Asp C700 fragment with resepct to the Mn-O bond, since both N and O ligators are behaving as anisotropic pi acceptors and donors, respectively. The orbital energies were used to gauge the ability of manganese to gain or loose an electron in an attempt to predict changes in the redox potential and to correlate these predictions with the data on the kinetics of dismutation recations. The resuls are used to explain our recent observation that E. coli Mn SOD exposed to nitric oxide (NO) in vitro catalyzes its dismutation.
PB  - Association of Greek Chemists
C3  - Book of abstracts - 5th International Symposium on Applied Bioinorganic Chemistry (5ISABC), Corfu, Greece
T1  - Ligand field regulation of manganese redox potential in MnSODs
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_cer_6538
ER  - 

@conference{
author = "Niketić, Svetozar and Niketić, Vesna and Stojanović, Srđan and Spasić, Mihajlo",
year = "1999",
abstract = "Ligand field analysis (in the AOM formalism) of Mn(ll, lll) in seven Mn SOD structures reported in the Brookhaven PDB show non-negligible differences in d-orbital energies. The results are equally affected by the azimuthal deformations of the coordination polyhedron, as well as by the orientations of the His imidazole rings with respect to the Mn-N bonds or of the Asp C700 fragment with resepct to the Mn-O bond, since both N and O ligators are behaving as anisotropic pi acceptors and donors, respectively. The orbital energies were used to gauge the ability of manganese to gain or loose an electron in an attempt to predict changes in the redox potential and to correlate these predictions with the data on the kinetics of dismutation recations. The resuls are used to explain our recent observation that E. coli Mn SOD exposed to nitric oxide (NO) in vitro catalyzes its dismutation.",
publisher = "Association of Greek Chemists",
journal = "Book of abstracts - 5th International Symposium on Applied Bioinorganic Chemistry (5ISABC), Corfu, Greece",
title = "Ligand field regulation of manganese redox potential in MnSODs",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_cer_6538"
}

Niketić, S., Niketić, V., Stojanović, S.,& Spasić, M.. (1999). Ligand field regulation of manganese redox potential in MnSODs. in Book of abstracts - 5th International Symposium on Applied Bioinorganic Chemistry (5ISABC), Corfu, Greece
Association of Greek Chemists., 52-52.
https://hdl.handle.net/21.15107/rcub_cer_6538

Niketić S, Niketić V, Stojanović S, Spasić M. Ligand field regulation of manganese redox potential in MnSODs. in Book of abstracts - 5th International Symposium on Applied Bioinorganic Chemistry (5ISABC), Corfu, Greece. 1999;:52-52.
https://hdl.handle.net/21.15107/rcub_cer_6538 .

Niketić, Svetozar, Niketić, Vesna, Stojanović, Srđan, Spasić, Mihajlo, "Ligand field regulation of manganese redox potential in MnSODs" in Book of abstracts - 5th International Symposium on Applied Bioinorganic Chemistry (5ISABC), Corfu, Greece (1999):52-52,
https://hdl.handle.net/21.15107/rcub_cer_6538 .

TY  - JOUR
AU  - Niketić, Vesna
AU  - Stojanović, Srđan
AU  - Nikolić, Aleksandra
AU  - Spasić, Mihajlo
AU  - Michelson, A.M.
PY  - 1999
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6508
AB  - The effect of NO treatment in vitro on structural and functional alterations of Cu/Zn, Mn, and Fe type of
SODs was studied. Significant difference in response to NO of Cu/ZnSOD compared to the Mn and Fe types was
demonstrated. Cu/ZnSOD was shown to be stable with respect to NO: even on prolonged exposure, NO produced
negligible effect on its structure and activity. In contrast, both Mn and Fe types were found to be NO-sensitive: exposure
to NO led to their fast and extensive inactivation, which was accompanied by extensive structural alterations, including
(in some of the samples tested) the cleavage of enzyme polypeptide chains, presumably at His residues of the enzyme
metal binding sites. The generation of nitrosonium (NO1) and nitroxyl (NO2) ions in NO treated Mn and FeSODs,
which produce enzyme modifications and inactivation, was demonstrated. The physiological and biomedical significance
of described findings is briefly discussed.
PB  - Elsevier
T2  - Free Radical Biology and Medicine
T1  - Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study
VL  - 27
IS  - 9-10
SP  - 992
EP  - 996
DO  - 10.1016/S0891-5849(98)00256-1
ER  - 

@article{
author = "Niketić, Vesna and Stojanović, Srđan and Nikolić, Aleksandra and Spasić, Mihajlo and Michelson, A.M.",
year = "1999",
abstract = "The effect of NO treatment in vitro on structural and functional alterations of Cu/Zn, Mn, and Fe type of
SODs was studied. Significant difference in response to NO of Cu/ZnSOD compared to the Mn and Fe types was
demonstrated. Cu/ZnSOD was shown to be stable with respect to NO: even on prolonged exposure, NO produced
negligible effect on its structure and activity. In contrast, both Mn and Fe types were found to be NO-sensitive: exposure
to NO led to their fast and extensive inactivation, which was accompanied by extensive structural alterations, including
(in some of the samples tested) the cleavage of enzyme polypeptide chains, presumably at His residues of the enzyme
metal binding sites. The generation of nitrosonium (NO1) and nitroxyl (NO2) ions in NO treated Mn and FeSODs,
which produce enzyme modifications and inactivation, was demonstrated. The physiological and biomedical significance
of described findings is briefly discussed.",
publisher = "Elsevier",
journal = "Free Radical Biology and Medicine",
title = "Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study",
volume = "27",
number = "9-10",
pages = "992-996",
doi = "10.1016/S0891-5849(98)00256-1"
}

Niketić, V., Stojanović, S., Nikolić, A., Spasić, M.,& Michelson, A.M.. (1999). Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study. in Free Radical Biology and Medicine
Elsevier., 27(9-10), 992-996.
https://doi.org/10.1016/S0891-5849(98)00256-1

Niketić V, Stojanović S, Nikolić A, Spasić M, Michelson A. Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study. in Free Radical Biology and Medicine. 1999;27(9-10):992-996.
doi:10.1016/S0891-5849(98)00256-1 .

Niketić, Vesna, Stojanović, Srđan, Nikolić, Aleksandra, Spasić, Mihajlo, Michelson, A.M., "Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study" in Free Radical Biology and Medicine, 27, no. 9-10 (1999):992-996,
https://doi.org/10.1016/S0891-5849(98)00256-1 . .

TY  - JOUR
AU  - Niketić, Vesna
AU  - Stojanović, Srđan
AU  - Spasić, Mihajlo
PY  - 1998
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3887
AB  - Recent evidence revealed that reactive oxygen species (ROS) such assuperoxide (O  ) and hydrogen peroxide (H O ), which are conventionally2.-22viewed as unwanted and toxic by-products of life in an aerobic environment,have  physiological  roles.  Appraisal  of  the  roles  of  reactive  NO  species(RNOS), such as nitrosonium (NO ) and nitroxyl (NO ) ions, peroxynitrite+-(OONO ), and higher nitrogen oxides (NO ) in NO mediated processes, is-xgrowing  at  a  rapid  rate.  ROS  and  RNOS  may  evoke  a  variety  of  cellularresponses, ranging from major changes in mammalian cell gene expressionto apoptotic death. The greater prevalence and reactivity of thiols over otherbiological nucleophiles makes them targets for both ROS and RNOS. Anyessential  protein  containing  cysteine  residue  that  is  strategically  located  ateither active or allosteric site should be considered as a target for regulationby  ROS  and/or  RNOS.  Candidate  molecules  include  proteins  that  are themselves involved in signal transduction processes, ion channels, receptors,G-proteins, protein-kinases, protein phosphatases, and transcription-activatingfactors. In this paper the mechanisms of generation of ROS and RNOS andtheir  reactions  with  thiols  are  briefly  described.  The  regulation  of  cellprocesses by ROS and RNOS is illustrated by the interference of ROS andRNOS effects with receptor tyrosine kinases signaling.
PB  - Yugoslav Union of Physiological Societies
T2  - Iugoslav. Physiol. Pharmacol. Acta
T1  - Regulation of cell processes by rective oxygen and nitric oxide species - Mechanisms of reactions
T1  - Regulacija ćelijskih procesa reaktivnim vrstama kiseonika i azot monoksida - Mehanizmi reakcija
VL  - 34
SP  - 463
EP  - 477
UR  - https://hdl.handle.net/21.15107/rcub_cer_3887
ER  - 

@article{
author = "Niketić, Vesna and Stojanović, Srđan and Spasić, Mihajlo",
year = "1998",
abstract = "Recent evidence revealed that reactive oxygen species (ROS) such assuperoxide (O  ) and hydrogen peroxide (H O ), which are conventionally2.-22viewed as unwanted and toxic by-products of life in an aerobic environment,have  physiological  roles.  Appraisal  of  the  roles  of  reactive  NO  species(RNOS), such as nitrosonium (NO ) and nitroxyl (NO ) ions, peroxynitrite+-(OONO ), and higher nitrogen oxides (NO ) in NO mediated processes, is-xgrowing  at  a  rapid  rate.  ROS  and  RNOS  may  evoke  a  variety  of  cellularresponses, ranging from major changes in mammalian cell gene expressionto apoptotic death. The greater prevalence and reactivity of thiols over otherbiological nucleophiles makes them targets for both ROS and RNOS. Anyessential  protein  containing  cysteine  residue  that  is  strategically  located  ateither active or allosteric site should be considered as a target for regulationby  ROS  and/or  RNOS.  Candidate  molecules  include  proteins  that  are themselves involved in signal transduction processes, ion channels, receptors,G-proteins, protein-kinases, protein phosphatases, and transcription-activatingfactors. In this paper the mechanisms of generation of ROS and RNOS andtheir  reactions  with  thiols  are  briefly  described.  The  regulation  of  cellprocesses by ROS and RNOS is illustrated by the interference of ROS andRNOS effects with receptor tyrosine kinases signaling.",
publisher = "Yugoslav Union of Physiological Societies",
journal = "Iugoslav. Physiol. Pharmacol. Acta",
title = "Regulation of cell processes by rective oxygen and nitric oxide species - Mechanisms of reactions, Regulacija ćelijskih procesa reaktivnim vrstama kiseonika i azot monoksida - Mehanizmi reakcija",
volume = "34",
pages = "463-477",
url = "https://hdl.handle.net/21.15107/rcub_cer_3887"
}

Niketić, V., Stojanović, S.,& Spasić, M.. (1998). Regulation of cell processes by rective oxygen and nitric oxide species - Mechanisms of reactions. in Iugoslav. Physiol. Pharmacol. Acta
Yugoslav Union of Physiological Societies., 34, 463-477.
https://hdl.handle.net/21.15107/rcub_cer_3887

Niketić V, Stojanović S, Spasić M. Regulation of cell processes by rective oxygen and nitric oxide species - Mechanisms of reactions. in Iugoslav. Physiol. Pharmacol. Acta. 1998;34:463-477.
https://hdl.handle.net/21.15107/rcub_cer_3887 .

Niketić, Vesna, Stojanović, Srđan, Spasić, Mihajlo, "Regulation of cell processes by rective oxygen and nitric oxide species - Mechanisms of reactions" in Iugoslav. Physiol. Pharmacol. Acta, 34 (1998):463-477,
https://hdl.handle.net/21.15107/rcub_cer_3887 .