TY  - JOUR
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Oreščanin-Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Stević, Zorica
PY  - 2020
UR  - https://www.eurekaselect.com/179582/article
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3966
AB  - Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.
PB  - Bentham Science
T2  - Current Drug Targets
T1  - Edaravone May Prevent Ferroptosis in ALS
VL  - 21
IS  - 8
SP  - 776
EP  - 780
DO  - 10.2174/1389450121666200220123305
ER  - 

@article{
author = "Spasić, Snežana and Nikolić-Kokić, Aleksandra and Miletić, Srđan and Oreščanin-Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško and Stević, Zorica",
year = "2020",
abstract = "Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.",
publisher = "Bentham Science",
journal = "Current Drug Targets",
title = "Edaravone May Prevent Ferroptosis in ALS",
volume = "21",
number = "8",
pages = "776-780",
doi = "10.2174/1389450121666200220123305"
}

Spasić, S., Nikolić-Kokić, A., Miletić, S., Oreščanin-Dušić, Z., Spasić, M., Blagojević, D.,& Stević, Z.. (2020). Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets
Bentham Science., 21(8), 776-780.
https://doi.org/10.2174/1389450121666200220123305

Spasić S, Nikolić-Kokić A, Miletić S, Oreščanin-Dušić Z, Spasić M, Blagojević D, Stević Z. Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets. 2020;21(8):776-780.
doi:10.2174/1389450121666200220123305 .

Spasić, Snežana, Nikolić-Kokić, Aleksandra, Miletić, Srđan, Oreščanin-Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, Stević, Zorica, "Edaravone May Prevent Ferroptosis in ALS" in Current Drug Targets, 21, no. 8 (2020):776-780,
https://doi.org/10.2174/1389450121666200220123305 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica
AU  - Spasić, Snežana
AU  - Jones, David R.
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/738
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Taylor & Francis Group
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
VL  - 15
IS  - 1
SP  - 29
EP  - 35
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica and Spasić, Snežana and Jones, David R. and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Taylor & Francis Group",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
volume = "15",
number = "1",
pages = "29-35",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z., Spasić, S., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Taylor & Francis Group., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević Z, Spasić S, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica, Spasić, Snežana, Jones, David R., Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Stević, Zorica
AU  - Stojanović, Srđan
AU  - Blagojević, Duško
AU  - Jones, David
AU  - Pavlović, Sanja
AU  - Nikrtić, Vesna
AU  - Apostolski, Slobodan
AU  - Spasić, Mihajlo
PY  - 2005
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3868
AB  - Recent findings indicate that nitric oxide (NO•) over-production might be an important factor in thepathogenesis  of  sporadic  amyotrophic  lateral  sclerosis  (SALS).  We  measured  significantly  higherconcentrations   of   uric   acid   and   thiol   group-containing   molecules   (R–SH   groups)   in   thecerebrospinal  fluid  (CSF)  from  SALS  patients  compared  to  controls.  The  above  factors,  togetherwith a slightly increased free iron concentration found in the CSF, favour conditions necessary forthe  formation  of  the  dinitrosyl  iron  complex,  capable  of  NO•bio-transformation.  Thus,  weperformed ex vivosaturation of CSF (from both SALS patients and controls) with NO•. A decreasein the level of R–SH was found. This was more pronounced in the CSF from SALS patients. In theCSF from SALS patients the production of nitrite and hydroxylamine was greater than that observedin the CSF from controls. Moreover, we also found increased Cu,Zn-SOD activity in the CSF fromSALS  patients  (when  compared  to  control  subjects)  but  no  activity  corresponding  to  Mn-SOD  inany CSF samples. As Cu,Zn-SOD can react with nitroxyl forming NO•, the conditions for a closed,but continuous, loop of NO•biotransformation are present in the CSF of ALS patients.
PB  - Maney Publishing
T2  - Redox Report
T1  - Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
VL  - 10
IS  - 5
SP  - 265
EP  - 270
DO  - 10.1179/135100005X70242
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Stević, Zorica and Stojanović, Srđan and Blagojević, Duško and Jones, David and Pavlović, Sanja and Nikrtić, Vesna and Apostolski, Slobodan and Spasić, Mihajlo",
year = "2005",
abstract = "Recent findings indicate that nitric oxide (NO•) over-production might be an important factor in thepathogenesis  of  sporadic  amyotrophic  lateral  sclerosis  (SALS).  We  measured  significantly  higherconcentrations   of   uric   acid   and   thiol   group-containing   molecules   (R–SH   groups)   in   thecerebrospinal  fluid  (CSF)  from  SALS  patients  compared  to  controls.  The  above  factors,  togetherwith a slightly increased free iron concentration found in the CSF, favour conditions necessary forthe  formation  of  the  dinitrosyl  iron  complex,  capable  of  NO•bio-transformation.  Thus,  weperformed ex vivosaturation of CSF (from both SALS patients and controls) with NO•. A decreasein the level of R–SH was found. This was more pronounced in the CSF from SALS patients. In theCSF from SALS patients the production of nitrite and hydroxylamine was greater than that observedin the CSF from controls. Moreover, we also found increased Cu,Zn-SOD activity in the CSF fromSALS  patients  (when  compared  to  control  subjects)  but  no  activity  corresponding  to  Mn-SOD  inany CSF samples. As Cu,Zn-SOD can react with nitroxyl forming NO•, the conditions for a closed,but continuous, loop of NO•biotransformation are present in the CSF of ALS patients.",
publisher = "Maney Publishing",
journal = "Redox Report",
title = "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
volume = "10",
number = "5",
pages = "265-270",
doi = "10.1179/135100005X70242"
}

Nikolić-Kokić, A., Stević, Z., Stojanović, S., Blagojević, D., Jones, D., Pavlović, S., Nikrtić, V., Apostolski, S.,& Spasić, M.. (2005). Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report
Maney Publishing., 10(5), 265-270.
https://doi.org/10.1179/135100005X70242

Nikolić-Kokić A, Stević Z, Stojanović S, Blagojević D, Jones D, Pavlović S, Nikrtić V, Apostolski S, Spasić M. Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2005;10(5):265-270.
doi:10.1179/135100005X70242 .

Nikolić-Kokić, Aleksandra, Stević, Zorica, Stojanović, Srđan, Blagojević, Duško, Jones, David, Pavlović, Sanja, Nikrtić, Vesna, Apostolski, Slobodan, Spasić, Mihajlo, "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 10, no. 5 (2005):265-270,
https://doi.org/10.1179/135100005X70242 . .