TY  - JOUR
AU  - Mišković, Jovana
AU  - Karaman, Maja A.
AU  - Rašeta, Milena J.
AU  - Krsmanović, Nenad
AU  - Berežni, Sanja
AU  - Jakovljević, Dragica
AU  - Piattoni, Federica
AU  - Zambonelli, Alessandra
AU  - Gargano, Maria Letizia
AU  - Venturella, Giuseppe
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4338
AB  - In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer’s disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different Schizophyllum commune strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC–MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC90 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC50 0.8 ± 0.6 µg/mL). PSH extracts (7 days) exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC–MS/MS analysis p-hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing S. commune as a valuable resource of AChE inhibitors.
PB  - MDPI
T2  - Journal of Fungi
T1  - Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation
VL  - 7
IS  - 2
SP  - 115
EP  - 17
DO  - 10.3390/jof7020115
ER  - 

@article{
author = "Mišković, Jovana and Karaman, Maja A. and Rašeta, Milena J. and Krsmanović, Nenad and Berežni, Sanja and Jakovljević, Dragica and Piattoni, Federica and Zambonelli, Alessandra and Gargano, Maria Letizia and Venturella, Giuseppe",
year = "2021",
abstract = "In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer’s disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different Schizophyllum commune strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC–MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC90 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC50 0.8 ± 0.6 µg/mL). PSH extracts (7 days) exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC–MS/MS analysis p-hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing S. commune as a valuable resource of AChE inhibitors.",
publisher = "MDPI",
journal = "Journal of Fungi",
title = "Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation",
volume = "7",
number = "2",
pages = "115-17",
doi = "10.3390/jof7020115"
}

Mišković, J., Karaman, M. A., Rašeta, M. J., Krsmanović, N., Berežni, S., Jakovljević, D., Piattoni, F., Zambonelli, A., Gargano, M. L.,& Venturella, G.. (2021). Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation. in Journal of Fungi
MDPI., 7(2), 115-17.
https://doi.org/10.3390/jof7020115

Mišković J, Karaman MA, Rašeta MJ, Krsmanović N, Berežni S, Jakovljević D, Piattoni F, Zambonelli A, Gargano ML, Venturella G. Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation. in Journal of Fungi. 2021;7(2):115-17.
doi:10.3390/jof7020115 .

Mišković, Jovana, Karaman, Maja A., Rašeta, Milena J., Krsmanović, Nenad, Berežni, Sanja, Jakovljević, Dragica, Piattoni, Federica, Zambonelli, Alessandra, Gargano, Maria Letizia, Venturella, Giuseppe, "Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation" in Journal of Fungi, 7, no. 2 (2021):115-17,
https://doi.org/10.3390/jof7020115 . .