Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation
2021
Преузимање 🢃
Аутори
Mišković, JovanaKaraman, Maja A.
Rašeta, Milena J.
Krsmanović, Nenad
Berežni, Sanja
Jakovljević, Dragica
Piattoni, Federica
Zambonelli, Alessandra
Gargano, Maria Letizia
Venturella, Giuseppe
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer’s disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different Schizophyllum commune strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC–MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC90 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC50 0.8 ± 0.6 µg/mL). PSH extracts (7 days) ...exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC–MS/MS analysis p-hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing S. commune as a valuable resource of AChE inhibitors.
Кључне речи:
Acetylcholinesterase inhibition / Antioxidant / Schizophyllum commune / Submerged cultivationИзвор:
Journal of Fungi, 2021, 7, 2, 115-17Издавач:
- MDPI
Финансирање / пројекти:
- Биосенсинг технологије и глобални систем за континуирана истраживања и интегрисано управљање екосистемима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43002)
- Биолошки активни природни производи као потенцијални извори нових лекова и дијететских суплемената (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.3390/jof7020115
ISSN: 2309-608X
PubMed: 33557141
WoS: 000622627700001
Scopus: 2-s2.0-85101159820
Институција/група
IHTMTY - JOUR AU - Mišković, Jovana AU - Karaman, Maja A. AU - Rašeta, Milena J. AU - Krsmanović, Nenad AU - Berežni, Sanja AU - Jakovljević, Dragica AU - Piattoni, Federica AU - Zambonelli, Alessandra AU - Gargano, Maria Letizia AU - Venturella, Giuseppe PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4338 AB - In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer’s disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different Schizophyllum commune strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC–MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC90 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC50 0.8 ± 0.6 µg/mL). PSH extracts (7 days) exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC–MS/MS analysis p-hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing S. commune as a valuable resource of AChE inhibitors. PB - MDPI T2 - Journal of Fungi T1 - Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation VL - 7 IS - 2 SP - 115 EP - 17 DO - 10.3390/jof7020115 ER -
@article{ author = "Mišković, Jovana and Karaman, Maja A. and Rašeta, Milena J. and Krsmanović, Nenad and Berežni, Sanja and Jakovljević, Dragica and Piattoni, Federica and Zambonelli, Alessandra and Gargano, Maria Letizia and Venturella, Giuseppe", year = "2021", abstract = "In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer’s disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different Schizophyllum commune strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC–MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC90 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC50 0.8 ± 0.6 µg/mL). PSH extracts (7 days) exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC–MS/MS analysis p-hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing S. commune as a valuable resource of AChE inhibitors.", publisher = "MDPI", journal = "Journal of Fungi", title = "Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation", volume = "7", number = "2", pages = "115-17", doi = "10.3390/jof7020115" }
Mišković, J., Karaman, M. A., Rašeta, M. J., Krsmanović, N., Berežni, S., Jakovljević, D., Piattoni, F., Zambonelli, A., Gargano, M. L.,& Venturella, G.. (2021). Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation. in Journal of Fungi MDPI., 7(2), 115-17. https://doi.org/10.3390/jof7020115
Mišković J, Karaman MA, Rašeta MJ, Krsmanović N, Berežni S, Jakovljević D, Piattoni F, Zambonelli A, Gargano ML, Venturella G. Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation. in Journal of Fungi. 2021;7(2):115-17. doi:10.3390/jof7020115 .
Mišković, Jovana, Karaman, Maja A., Rašeta, Milena J., Krsmanović, Nenad, Berežni, Sanja, Jakovljević, Dragica, Piattoni, Federica, Zambonelli, Alessandra, Gargano, Maria Letizia, Venturella, Giuseppe, "Comparison of two schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation" in Journal of Fungi, 7, no. 2 (2021):115-17, https://doi.org/10.3390/jof7020115 . .