TY  - JOUR
AU  - Medić, Ana
AU  - Huttmann, Nico
AU  - Lješević, Marija
AU  - Risha, Yousef
AU  - Berezovski, Maxim
AU  - Minić, Zoran
AU  - Karadžić, Ivanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5548
AB  - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
PB  - Elsevier
T2  - Microbiological Research
T1  - A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform
VL  - 259
SP  - 126998
DO  - 10.1016/j.micres.2022.126998
ER  - 

@article{
author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka",
year = "2022",
abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.",
publisher = "Elsevier",
journal = "Microbiological Research",
title = "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform",
volume = "259",
pages = "126998",
doi = "10.1016/j.micres.2022.126998"
}

Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research
Elsevier., 259, 126998.
https://doi.org/10.1016/j.micres.2022.126998

Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998.
doi:10.1016/j.micres.2022.126998 .

Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998,
https://doi.org/10.1016/j.micres.2022.126998 . .

TY  - JOUR
AU  - Medić, Ana
AU  - Huttmann, Nico
AU  - Lješević, Marija
AU  - Risha, Yousef
AU  - Berezovski, Maxim
AU  - Minić, Zoran
AU  - Karadžić, Ivanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5190
AB  - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
PB  - Elsevier
T2  - Microbiological Research
T1  - A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform
VL  - 259
SP  - 126998
DO  - 10.1016/j.micres.2022.126998
ER  - 

@article{
author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka",
year = "2022",
abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.",
publisher = "Elsevier",
journal = "Microbiological Research",
title = "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform",
volume = "259",
pages = "126998",
doi = "10.1016/j.micres.2022.126998"
}

Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research
Elsevier., 259, 126998.
https://doi.org/10.1016/j.micres.2022.126998

Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998.
doi:10.1016/j.micres.2022.126998 .

Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998,
https://doi.org/10.1016/j.micres.2022.126998 . .

TY  - CONF
AU  - Medić, A.
AU  - Lješević, Marija
AU  - Karadžić, Ivanka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5126
AB  - Poster presented at 21st European Meeting on Environmental Chemistry (EMEC21), 30 November-3 december 2021, Novi Sad, Serbia
T1  - Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai
UR  - https://hdl.handle.net/21.15107/rcub_cer_5126
ER  - 

@conference{
author = "Medić, A. and Lješević, Marija and Karadžić, Ivanka",
year = "2021",
abstract = "Poster presented at 21st European Meeting on Environmental Chemistry (EMEC21), 30 November-3 december 2021, Novi Sad, Serbia",
title = "Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai",
url = "https://hdl.handle.net/21.15107/rcub_cer_5126"
}

Medić, A., Lješević, M.,& Karadžić, I.. (2021). Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai. .
https://hdl.handle.net/21.15107/rcub_cer_5126

Medić A, Lješević M, Karadžić I. Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai. 2021;.
https://hdl.handle.net/21.15107/rcub_cer_5126 .

Medić, A., Lješević, Marija, Karadžić, Ivanka, "Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai" (2021),
https://hdl.handle.net/21.15107/rcub_cer_5126 .

TY  - CONF
AU  - Medić, A.
AU  - Lješević, Marija
AU  - Karadžić, Ivanka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5125
AB  - The goal of this study was to analyze the products of benzoate degradation by polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai using targeted metabolomic analysis in order to determine the specificity of the metabolic pathway of sodium-benzoate.
PB  - Belgrade : Serbian Chemical Society
C3  - 21st European Meeting on Environmental Chemistry (EMEC21), Book of abstracts, 30 November-3 december 2021, Novi Sad, Serbia
T1  - Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_cer_5125
ER  - 

@conference{
author = "Medić, A. and Lješević, Marija and Karadžić, Ivanka",
year = "2021",
abstract = "The goal of this study was to analyze the products of benzoate degradation by polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai using targeted metabolomic analysis in order to determine the specificity of the metabolic pathway of sodium-benzoate.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "21st European Meeting on Environmental Chemistry (EMEC21), Book of abstracts, 30 November-3 december 2021, Novi Sad, Serbia",
title = "Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_cer_5125"
}

Medić, A., Lješević, M.,& Karadžić, I.. (2021). Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai. in 21st European Meeting on Environmental Chemistry (EMEC21), Book of abstracts, 30 November-3 december 2021, Novi Sad, Serbia
Belgrade : Serbian Chemical Society., 160.
https://hdl.handle.net/21.15107/rcub_cer_5125

Medić A, Lješević M, Karadžić I. Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai. in 21st European Meeting on Environmental Chemistry (EMEC21), Book of abstracts, 30 November-3 december 2021, Novi Sad, Serbia. 2021;:160.
https://hdl.handle.net/21.15107/rcub_cer_5125 .

Medić, A., Lješević, Marija, Karadžić, Ivanka, "Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai" in 21st European Meeting on Environmental Chemistry (EMEC21), Book of abstracts, 30 November-3 december 2021, Novi Sad, Serbia (2021):160,
https://hdl.handle.net/21.15107/rcub_cer_5125 .

TY  - JOUR
AU  - Medić, Ana
AU  - Lješević, Marija
AU  - Inui, Hideyuku
AU  - Beškoski, Vladimir
AU  - Kojić, Ivan
AU  - Stojanović, Ksenija
AU  - Karadžić, Ivanka
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3576
AB  - Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected
petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic
hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%,
respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high
biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from
crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached
stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum
biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the
terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for
fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with
multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental
bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or
elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.
PB  - Royal Society  of Chemistry
T2  - RSC Advances
T1  - Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity
VL  - 10
IS  - 24
SP  - 14060
EP  - 14070
DO  - 10.1039/c9ra10371f
ER  - 

@article{
author = "Medić, Ana and Lješević, Marija and Inui, Hideyuku and Beškoski, Vladimir and Kojić, Ivan and Stojanović, Ksenija and Karadžić, Ivanka",
year = "2020",
abstract = "Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected
petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic
hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%,
respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high
biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from
crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached
stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum
biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the
terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for
fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with
multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental
bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or
elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.",
publisher = "Royal Society  of Chemistry",
journal = "RSC Advances",
title = "Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity",
volume = "10",
number = "24",
pages = "14060-14070",
doi = "10.1039/c9ra10371f"
}

Medić, A., Lješević, M., Inui, H., Beškoski, V., Kojić, I., Stojanović, K.,& Karadžić, I.. (2020). Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity. in RSC Advances
Royal Society  of Chemistry., 10(24), 14060-14070.
https://doi.org/10.1039/c9ra10371f

Medić A, Lješević M, Inui H, Beškoski V, Kojić I, Stojanović K, Karadžić I. Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity. in RSC Advances. 2020;10(24):14060-14070.
doi:10.1039/c9ra10371f .

Medić, Ana, Lješević, Marija, Inui, Hideyuku, Beškoski, Vladimir, Kojić, Ivan, Stojanović, Ksenija, Karadžić, Ivanka, "Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity" in RSC Advances, 10, no. 24 (2020):14060-14070,
https://doi.org/10.1039/c9ra10371f . .

TY  - JOUR
AU  - Izrael‑Živković, Lidija
AU  - Beškoski, Vladimir
AU  - Rikalović, Milena
AU  - Kazazić, Snježana
AU  - Shapiro, Nicole
AU  - Woyke, Tanja
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
AU  - Maksimović, Nela
AU  - Karadžić, Ivanka
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3026
AB  - The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.
PB  - Springer
T2  - Extremophiles
T1  - High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals
VL  - 23
IS  - 4
SP  - 399
EP  - 405
DO  - 10.1007/s00792-019-01092-w
ER  - 

@article{
author = "Izrael‑Živković, Lidija and Beškoski, Vladimir and Rikalović, Milena and Kazazić, Snježana and Shapiro, Nicole and Woyke, Tanja and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Maksimović, Nela and Karadžić, Ivanka",
year = "2019",
abstract = "The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions andinvolved in catabolism of toxic aromatic organic compounds were identified. P.aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.",
publisher = "Springer",
journal = "Extremophiles",
title = "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals",
volume = "23",
number = "4",
pages = "399-405",
doi = "10.1007/s00792-019-01092-w"
}

Izrael‑Živković, L., Beškoski, V., Rikalović, M., Kazazić, S., Shapiro, N., Woyke, T., Gojgić-Cvijović, G., Vrvić, M., Maksimović, N.,& Karadžić, I.. (2019). High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles
Springer., 23(4), 399-405.
https://doi.org/10.1007/s00792-019-01092-w

Izrael‑Živković L, Beškoski V, Rikalović M, Kazazić S, Shapiro N, Woyke T, Gojgić-Cvijović G, Vrvić M, Maksimović N, Karadžić I. High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. in Extremophiles. 2019;23(4):399-405.
doi:10.1007/s00792-019-01092-w .

Izrael‑Živković, Lidija, Beškoski, Vladimir, Rikalović, Milena, Kazazić, Snježana, Shapiro, Nicole, Woyke, Tanja, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Maksimović, Nela, Karadžić, Ivanka, "High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals" in Extremophiles, 23, no. 4 (2019):399-405,
https://doi.org/10.1007/s00792-019-01092-w . .

TY  - JOUR
AU  - Medić, Ana
AU  - Stojanović, Ksenija
AU  - Izrael-Živković, Lidija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka
AU  - Kazazić, Saša
AU  - Karadžić, Ivanka
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3214
AB  - The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This
investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size,
pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the
pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and
100 mg L 1
, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme
preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal
than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of
aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data,
a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was
conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the
upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests;
therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A
comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to
aromatic substances can be summarized as the upregulation of proteins responsible for amino acid
metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes
of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic
compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated
areas
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai
VL  - 9
IS  - 41
SP  - 23696
EP  - 23710
DO  - 10.1039/c9ra04298a
ER  - 

@article{
author = "Medić, Ana and Stojanović, Ksenija and Izrael-Živković, Lidija and Beškoski, Vladimir and Lončarević, Branka and Kazazić, Saša and Karadžić, Ivanka",
year = "2019",
abstract = "The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This
investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size,
pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the
pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and
100 mg L 1
, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme
preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal
than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of
aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data,
a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was
conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the
upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests;
therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A
comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to
aromatic substances can be summarized as the upregulation of proteins responsible for amino acid
metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes
of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic
compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated
areas",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai",
volume = "9",
number = "41",
pages = "23696-23710",
doi = "10.1039/c9ra04298a"
}

Medić, A., Stojanović, K., Izrael-Živković, L., Beškoski, V., Lončarević, B., Kazazić, S.,& Karadžić, I.. (2019). A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances
Royal Society of Chemistry., 9(41), 23696-23710.
https://doi.org/10.1039/c9ra04298a

Medić A, Stojanović K, Izrael-Živković L, Beškoski V, Lončarević B, Kazazić S, Karadžić I. A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances. 2019;9(41):23696-23710.
doi:10.1039/c9ra04298a .

Medić, Ana, Stojanović, Ksenija, Izrael-Živković, Lidija, Beškoski, Vladimir, Lončarević, Branka, Kazazić, Saša, Karadžić, Ivanka, "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai" in RSC Advances, 9, no. 41 (2019):23696-23710,
https://doi.org/10.1039/c9ra04298a . .

TY  - JOUR
AU  - Izrael‑Živković, Lidija
AU  - Rikalović, Milena
AU  - Gojgić-Cvijović, Gordana
AU  - Kazazic, Sasa
AU  - Vrvić, Miroslav
AU  - Brčeski, Ilija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka
AU  - Gopcevic, Kristina
AU  - Karadžić, Ivanka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2347
AB  - Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai
VL  - 8
IS  - 19
SP  - 10549
EP  - 10560
DO  - 10.1039/c8ra00371h
ER  - 

@article{
author = "Izrael‑Živković, Lidija and Rikalović, Milena and Gojgić-Cvijović, Gordana and Kazazic, Sasa and Vrvić, Miroslav and Brčeski, Ilija and Beškoski, Vladimir and Lončarević, Branka and Gopcevic, Kristina and Karadžić, Ivanka",
year = "2018",
abstract = "Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai",
volume = "8",
number = "19",
pages = "10549-10560",
doi = "10.1039/c8ra00371h"
}

Izrael‑Živković, L., Rikalović, M., Gojgić-Cvijović, G., Kazazic, S., Vrvić, M., Brčeski, I., Beškoski, V., Lončarević, B., Gopcevic, K.,& Karadžić, I.. (2018). Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. in RSC Advances
Royal Soc Chemistry, Cambridge., 8(19), 10549-10560.
https://doi.org/10.1039/c8ra00371h

Izrael‑Živković L, Rikalović M, Gojgić-Cvijović G, Kazazic S, Vrvić M, Brčeski I, Beškoski V, Lončarević B, Gopcevic K, Karadžić I. Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. in RSC Advances. 2018;8(19):10549-10560.
doi:10.1039/c8ra00371h .

Izrael‑Živković, Lidija, Rikalović, Milena, Gojgić-Cvijović, Gordana, Kazazic, Sasa, Vrvić, Miroslav, Brčeski, Ilija, Beškoski, Vladimir, Lončarević, Branka, Gopcevic, Kristina, Karadžić, Ivanka, "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai" in RSC Advances, 8, no. 19 (2018):10549-10560,
https://doi.org/10.1039/c8ra00371h . .

TY  - JOUR
AU  - Rikalović, Milena
AU  - Abdel-Mawgoud, Ahmad Mohammad
AU  - Déziel, Eric
AU  - Gojgić-Cvijović, Gordana
AU  - Nestorović, Zorica
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1374
AB  - A comparative analysis of rhamnolipids from environmental isolates of Pseudomonas aeruginosa was undertaken to evaluate strain-specific rhamnolipid fingerprints obtained under different growth conditions. Environmental isolates of P. aeruginosa produced rhamnolipids on different types of substrates, including cheap and renewable sources like sunflower oil from deep fryers and sunflower oil mill effluent. Rhamnolipids were monitored by high-performance liquid chromatography-electrospray ionization interface mass spectrometry, which allowed fast and reliable identification and quantification of the congeners present. The highest concentration of total rhamnolipids of 3.33 g/l was obtained by the strain P. aeruginosa 67, recovered from petroleum contaminated soil, and strains D1 (1.73 g/l) and D2 (1.70 g/l), recovered from natural microbial consortia originated from mazut-contaminated soil, grown on sunflower oil as a carbon source. Di- to mono-rhamnolipids ratios were in the range of 0.90-5.39 for different media composition and from 1.12 to 4.17 for different producing strains. Rhamnolipid profiles of purified mixtures of all tested strains are similar with chain length from C8-C12, pronounced abundance of Rha-C10-C10 and Rha-Rha-C 10-C10 congeners, and a low content of 3-(3-hydroxyalkanoyloxy)-alkanoic acids. Concentrations of major congeners of RLs were found to slightly vary, depending on strain and growth conditions, while variations in minor congeners were more pronounced. Statistically significant increase of critical micelle concentration values was observed with lowering the ratio of total mono- to di-rhamnolipids ratio indicating that mono-rhamnolipids start to form micelles at lower concentration than di-rhamnolipids.
T2  - Journal of Surfactants and Detergents
T1  - Comparative analysis of rhamnolipids from novel environmental isolates of Pseudomonas aeruginosa
VL  - 16
IS  - 5
SP  - 673
EP  - 682
DO  - 10.1007/s11743-013-1462-4
ER  - 

@article{
author = "Rikalović, Milena and Abdel-Mawgoud, Ahmad Mohammad and Déziel, Eric and Gojgić-Cvijović, Gordana and Nestorović, Zorica and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2013",
abstract = "A comparative analysis of rhamnolipids from environmental isolates of Pseudomonas aeruginosa was undertaken to evaluate strain-specific rhamnolipid fingerprints obtained under different growth conditions. Environmental isolates of P. aeruginosa produced rhamnolipids on different types of substrates, including cheap and renewable sources like sunflower oil from deep fryers and sunflower oil mill effluent. Rhamnolipids were monitored by high-performance liquid chromatography-electrospray ionization interface mass spectrometry, which allowed fast and reliable identification and quantification of the congeners present. The highest concentration of total rhamnolipids of 3.33 g/l was obtained by the strain P. aeruginosa 67, recovered from petroleum contaminated soil, and strains D1 (1.73 g/l) and D2 (1.70 g/l), recovered from natural microbial consortia originated from mazut-contaminated soil, grown on sunflower oil as a carbon source. Di- to mono-rhamnolipids ratios were in the range of 0.90-5.39 for different media composition and from 1.12 to 4.17 for different producing strains. Rhamnolipid profiles of purified mixtures of all tested strains are similar with chain length from C8-C12, pronounced abundance of Rha-C10-C10 and Rha-Rha-C 10-C10 congeners, and a low content of 3-(3-hydroxyalkanoyloxy)-alkanoic acids. Concentrations of major congeners of RLs were found to slightly vary, depending on strain and growth conditions, while variations in minor congeners were more pronounced. Statistically significant increase of critical micelle concentration values was observed with lowering the ratio of total mono- to di-rhamnolipids ratio indicating that mono-rhamnolipids start to form micelles at lower concentration than di-rhamnolipids.",
journal = "Journal of Surfactants and Detergents",
title = "Comparative analysis of rhamnolipids from novel environmental isolates of Pseudomonas aeruginosa",
volume = "16",
number = "5",
pages = "673-682",
doi = "10.1007/s11743-013-1462-4"
}

Rikalović, M., Abdel-Mawgoud, A. M., Déziel, E., Gojgić-Cvijović, G., Nestorović, Z., Vrvić, M.,& Karadžić, I.. (2013). Comparative analysis of rhamnolipids from novel environmental isolates of Pseudomonas aeruginosa. in Journal of Surfactants and Detergents, 16(5), 673-682.
https://doi.org/10.1007/s11743-013-1462-4

Rikalović M, Abdel-Mawgoud AM, Déziel E, Gojgić-Cvijović G, Nestorović Z, Vrvić M, Karadžić I. Comparative analysis of rhamnolipids from novel environmental isolates of Pseudomonas aeruginosa. in Journal of Surfactants and Detergents. 2013;16(5):673-682.
doi:10.1007/s11743-013-1462-4 .

Rikalović, Milena, Abdel-Mawgoud, Ahmad Mohammad, Déziel, Eric, Gojgić-Cvijović, Gordana, Nestorović, Zorica, Vrvić, Miroslav, Karadžić, Ivanka, "Comparative analysis of rhamnolipids from novel environmental isolates of Pseudomonas aeruginosa" in Journal of Surfactants and Detergents, 16, no. 5 (2013):673-682,
https://doi.org/10.1007/s11743-013-1462-4 . .

TY  - JOUR
AU  - Bihelović, Filip
AU  - Karadžić, Ivanka
AU  - Matović, Radomir
AU  - Saičić, Radomir N.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1214
AB  - Enantioselective synthesis of a marine antibiotic (-)-atrop-abyssomicin C was accomplished in 21 steps, in 1.8% overall yield (4%, based on the recovered starting material). The key steps of the synthesis are the formation of the functionalized cyclohexane core by an organocatalyzed Tsuji-Trost reaction, the formation of a tricyclic spirotetronate unit by a gold-catalyzed reaction sequence and the highly efficient eleven-membered ring closure by a Nozaki-Hiyama-Kishi reaction. Biological tests showed all abyssomicin derivatives to possess strong antibacterial activity against methicillin resistant S. aureus strains; however, they also proved to be cytotoxic, both to malignant and to normal somatic cells.
PB  - Royal Soc Chemistry, Cambridge
T2  - Organic & Biomolecular Chemistry
T1  - Total synthesis and biological evaluation of (-)-atrop-abyssomicin C
VL  - 11
IS  - 33
SP  - 5413
EP  - 5424
DO  - 10.1039/c3ob40692j
ER  - 

@article{
author = "Bihelović, Filip and Karadžić, Ivanka and Matović, Radomir and Saičić, Radomir N.",
year = "2013",
abstract = "Enantioselective synthesis of a marine antibiotic (-)-atrop-abyssomicin C was accomplished in 21 steps, in 1.8% overall yield (4%, based on the recovered starting material). The key steps of the synthesis are the formation of the functionalized cyclohexane core by an organocatalyzed Tsuji-Trost reaction, the formation of a tricyclic spirotetronate unit by a gold-catalyzed reaction sequence and the highly efficient eleven-membered ring closure by a Nozaki-Hiyama-Kishi reaction. Biological tests showed all abyssomicin derivatives to possess strong antibacterial activity against methicillin resistant S. aureus strains; however, they also proved to be cytotoxic, both to malignant and to normal somatic cells.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Organic & Biomolecular Chemistry",
title = "Total synthesis and biological evaluation of (-)-atrop-abyssomicin C",
volume = "11",
number = "33",
pages = "5413-5424",
doi = "10.1039/c3ob40692j"
}

Bihelović, F., Karadžić, I., Matović, R.,& Saičić, R. N.. (2013). Total synthesis and biological evaluation of (-)-atrop-abyssomicin C. in Organic & Biomolecular Chemistry
Royal Soc Chemistry, Cambridge., 11(33), 5413-5424.
https://doi.org/10.1039/c3ob40692j

Bihelović F, Karadžić I, Matović R, Saičić RN. Total synthesis and biological evaluation of (-)-atrop-abyssomicin C. in Organic & Biomolecular Chemistry. 2013;11(33):5413-5424.
doi:10.1039/c3ob40692j .

Bihelović, Filip, Karadžić, Ivanka, Matović, Radomir, Saičić, Radomir N., "Total synthesis and biological evaluation of (-)-atrop-abyssomicin C" in Organic & Biomolecular Chemistry, 11, no. 33 (2013):5413-5424,
https://doi.org/10.1039/c3ob40692j . .

TY  - GEN
AU  - Izrael-Živković, Lidija
AU  - Karadžić, Ivanka
AU  - Gojgić-Cvijović, Gordana
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5090
AB  - Tehničko rešenje je iz oblasti mikrobiološke proizvodnje enzima. Odnosi se na nov i ekonomičan postupak fermentacije za proizvodnju lipaze. Tehničko rešenje omogućava proizvodnju enzima koji je stabilan i aktivan u organskim rastvaračima. Lipaza se može primeniti u hidrolizi i sintezi (u nevodenoj sredini) složenih lipidnih molekula.
PB  - University of Belgrade - Institute of Chemistry, Technology and Metallurgy
T1  - Novi tehnološki postupak za dobijanje mikrobne lipaze pomoću Pseudomonas aeruginosa NCAIM(P)B 001380
UR  - https://hdl.handle.net/21.15107/rcub_cer_5090
ER  - 

@misc{
author = "Izrael-Živković, Lidija and Karadžić, Ivanka and Gojgić-Cvijović, Gordana and Rikalović, Milena and Vrvić, Miroslav",
year = "2012",
abstract = "Tehničko rešenje je iz oblasti mikrobiološke proizvodnje enzima. Odnosi se na nov i ekonomičan postupak fermentacije za proizvodnju lipaze. Tehničko rešenje omogućava proizvodnju enzima koji je stabilan i aktivan u organskim rastvaračima. Lipaza se može primeniti u hidrolizi i sintezi (u nevodenoj sredini) složenih lipidnih molekula.",
publisher = "University of Belgrade - Institute of Chemistry, Technology and Metallurgy",
title = "Novi tehnološki postupak za dobijanje mikrobne lipaze pomoću Pseudomonas aeruginosa NCAIM(P)B 001380",
url = "https://hdl.handle.net/21.15107/rcub_cer_5090"
}

Izrael-Živković, L., Karadžić, I., Gojgić-Cvijović, G., Rikalović, M.,& Vrvić, M.. (2012). Novi tehnološki postupak za dobijanje mikrobne lipaze pomoću Pseudomonas aeruginosa NCAIM(P)B 001380. 
University of Belgrade - Institute of Chemistry, Technology and Metallurgy..
https://hdl.handle.net/21.15107/rcub_cer_5090

Izrael-Živković L, Karadžić I, Gojgić-Cvijović G, Rikalović M, Vrvić M. Novi tehnološki postupak za dobijanje mikrobne lipaze pomoću Pseudomonas aeruginosa NCAIM(P)B 001380. 2012;.
https://hdl.handle.net/21.15107/rcub_cer_5090 .

Izrael-Živković, Lidija, Karadžić, Ivanka, Gojgić-Cvijović, Gordana, Rikalović, Milena, Vrvić, Miroslav, "Novi tehnološki postupak za dobijanje mikrobne lipaze pomoću Pseudomonas aeruginosa NCAIM(P)B 001380" (2012),
https://hdl.handle.net/21.15107/rcub_cer_5090 .

TY  - JOUR
AU  - Rikalović, Milena
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/998
AB  - The production and characteristics of rhamnolipid biosurfactant obtained by the strain Pseudomonas aeruginosa san-ai were investigated. With regard to the carbon and nitrogen sources, several media were tested to enhance the production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sunflower oil as a source of carbon and mineral ammonium chloride and peptone as nitrogen sources greatly improved the production of rhamnolipid, from 0.15 on basic PPAS (C/N ratio 4.0) to 3 g L-1 on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing the effect of three factors, i.e., temperature, concentration of carbon and nitrogen source (mass %), in the optimized PPAS medium on the production of rhamnolipid. The isolated rhamnolipids were characterized by infrared (IR) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The IR spectra confirmed that the isolated compound corresponded to the rhamnolipid structure, whereas MS indicated that the isolated preparation was a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners.
AB  - U ovom radu je opisana produkcija i karakterizacija ramnolipidnog biosurfaktanta soja Pseudomonas aeruginosa san-ai. Ispitan je uticaj različitih izvora ugljenika i azota na produkciju ramnolipida. Podloga proteoze pepton-amonijum soli sa ograničenom količinom fosfata (PPAS) sa suncokretovim uljem kao izvorom ugljenika i amonijum-hloridom i peptonom kao izvorom azota, znatno povećava produkciju ramnolipida, od 0,15, na PPAS sa standardnim sastavom (odnos C/N 4,0), do 3 g L-1 na optimizovanoj PPAS podlozi (odnos C/N 7,7). Metodom planiranog eksperimenta (response surface methodology) ispitan je efekat tri parametra: temperature, koncentracije izvora ugljenika i azota na optimizovanoj podlozi. Karakterizacija izolovanog preparata ramnolipida urađena je pomoću IR i ESI-MS analiza. IR analiza je potvrdila prisustvo strukturnih elemenata karakterističnih za ramnolipide. MS analiza pokazala je da reč o smeši u kojoj su prisutne mono-ramno-monolipidne, di-ramno-mono-lipidne i di-ramno-di-lipidne komponente.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai
T1  - Produkcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-ai
VL  - 77
IS  - 1
SP  - 27
EP  - 42
DO  - 10.2298/JSC110211156R
ER  - 

@article{
author = "Rikalović, Milena and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2012",
abstract = "The production and characteristics of rhamnolipid biosurfactant obtained by the strain Pseudomonas aeruginosa san-ai were investigated. With regard to the carbon and nitrogen sources, several media were tested to enhance the production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sunflower oil as a source of carbon and mineral ammonium chloride and peptone as nitrogen sources greatly improved the production of rhamnolipid, from 0.15 on basic PPAS (C/N ratio 4.0) to 3 g L-1 on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing the effect of three factors, i.e., temperature, concentration of carbon and nitrogen source (mass %), in the optimized PPAS medium on the production of rhamnolipid. The isolated rhamnolipids were characterized by infrared (IR) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The IR spectra confirmed that the isolated compound corresponded to the rhamnolipid structure, whereas MS indicated that the isolated preparation was a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners., U ovom radu je opisana produkcija i karakterizacija ramnolipidnog biosurfaktanta soja Pseudomonas aeruginosa san-ai. Ispitan je uticaj različitih izvora ugljenika i azota na produkciju ramnolipida. Podloga proteoze pepton-amonijum soli sa ograničenom količinom fosfata (PPAS) sa suncokretovim uljem kao izvorom ugljenika i amonijum-hloridom i peptonom kao izvorom azota, znatno povećava produkciju ramnolipida, od 0,15, na PPAS sa standardnim sastavom (odnos C/N 4,0), do 3 g L-1 na optimizovanoj PPAS podlozi (odnos C/N 7,7). Metodom planiranog eksperimenta (response surface methodology) ispitan je efekat tri parametra: temperature, koncentracije izvora ugljenika i azota na optimizovanoj podlozi. Karakterizacija izolovanog preparata ramnolipida urađena je pomoću IR i ESI-MS analiza. IR analiza je potvrdila prisustvo strukturnih elemenata karakterističnih za ramnolipide. MS analiza pokazala je da reč o smeši u kojoj su prisutne mono-ramno-monolipidne, di-ramno-mono-lipidne i di-ramno-di-lipidne komponente.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai, Produkcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-ai",
volume = "77",
number = "1",
pages = "27-42",
doi = "10.2298/JSC110211156R"
}

Rikalović, M., Gojgić-Cvijović, G., Vrvić, M.,& Karadžić, I.. (2012). Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 77(1), 27-42.
https://doi.org/10.2298/JSC110211156R

Rikalović M, Gojgić-Cvijović G, Vrvić M, Karadžić I. Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai. in Journal of the Serbian Chemical Society. 2012;77(1):27-42.
doi:10.2298/JSC110211156R .

Rikalović, Milena, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Karadžić, Ivanka, "Production and characterization of rhamnolipids from Pseudomonas aeruginosa san-ai" in Journal of the Serbian Chemical Society, 77, no. 1 (2012):27-42,
https://doi.org/10.2298/JSC110211156R . .

TY  - CONF
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5297
AB  - Poster presented at: 16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia
T1  - Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS
UR  - https://hdl.handle.net/21.15107/rcub_cer_5297
ER  - 

@conference{
author = "Rikalović, Milena and Vrvić, Miroslav and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2011",
abstract = "Poster presented at: 16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia",
title = "Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS",
url = "https://hdl.handle.net/21.15107/rcub_cer_5297"
}

Rikalović, M., Vrvić, M., Gojgić-Cvijović, G.,& Karadžić, I.. (2011). Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS. .
https://hdl.handle.net/21.15107/rcub_cer_5297

Rikalović M, Vrvić M, Gojgić-Cvijović G, Karadžić I. Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS. 2011;.
https://hdl.handle.net/21.15107/rcub_cer_5297 .

Rikalović, Milena, Vrvić, Miroslav, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS" (2011),
https://hdl.handle.net/21.15107/rcub_cer_5297 .

TY  - CONF
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5301
AB  - Poster presented at: 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011
T1  - Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai
UR  - https://hdl.handle.net/21.15107/rcub_cer_5301
ER  - 

@conference{
author = "Rikalović, Milena and Vrvić, Miroslav and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2011",
abstract = "Poster presented at: 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011",
title = "Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai",
url = "https://hdl.handle.net/21.15107/rcub_cer_5301"
}

Rikalović, M., Vrvić, M., Gojgić-Cvijović, G.,& Karadžić, I.. (2011). Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai. .
https://hdl.handle.net/21.15107/rcub_cer_5301

Rikalović M, Vrvić M, Gojgić-Cvijović G, Karadžić I. Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai. 2011;.
https://hdl.handle.net/21.15107/rcub_cer_5301 .

Rikalović, Milena, Vrvić, Miroslav, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai" (2011),
https://hdl.handle.net/21.15107/rcub_cer_5301 .

TY  - CONF
AU  - Izrael-Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Natić, Maja
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5201
AB  - Poster presented at 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011
T1  - Pseudomonas lipase catalyzed synthesis of ergosterol oleate
UR  - https://hdl.handle.net/21.15107/rcub_cer_5201
ER  - 

@conference{
author = "Izrael-Živković, Lidija and Gojgić-Cvijović, Gordana and Natić, Maja and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2011",
abstract = "Poster presented at 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011",
title = "Pseudomonas lipase catalyzed synthesis of ergosterol oleate",
url = "https://hdl.handle.net/21.15107/rcub_cer_5201"
}

Izrael-Živković, L., Gojgić-Cvijović, G., Natić, M., Vrvić, M.,& Karadžić, I.. (2011). Pseudomonas lipase catalyzed synthesis of ergosterol oleate. .
https://hdl.handle.net/21.15107/rcub_cer_5201

Izrael-Živković L, Gojgić-Cvijović G, Natić M, Vrvić M, Karadžić I. Pseudomonas lipase catalyzed synthesis of ergosterol oleate. 2011;.
https://hdl.handle.net/21.15107/rcub_cer_5201 .

Izrael-Živković, Lidija, Gojgić-Cvijović, Gordana, Natić, Maja, Vrvić, Miroslav, Karadžić, Ivanka, "Pseudomonas lipase catalyzed synthesis of ergosterol oleate" (2011),
https://hdl.handle.net/21.15107/rcub_cer_5201 .

TY  - CONF
AU  - Rikalović, Milena
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5139
AB  - Poster presented at: 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011)
T1  - MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380
UR  - https://hdl.handle.net/21.15107/rcub_cer_5139
ER  - 

@conference{
author = "Rikalović, Milena and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2011",
abstract = "Poster presented at: 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011)",
title = "MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380",
url = "https://hdl.handle.net/21.15107/rcub_cer_5139"
}

Rikalović, M., Gojgić-Cvijović, G., Vrvić, M.,& Karadžić, I.. (2011). MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380. .
https://hdl.handle.net/21.15107/rcub_cer_5139

Rikalović M, Gojgić-Cvijović G, Vrvić M, Karadžić I. MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380. 2011;.
https://hdl.handle.net/21.15107/rcub_cer_5139 .

Rikalović, Milena, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Karadžić, Ivanka, "MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380" (2011),
https://hdl.handle.net/21.15107/rcub_cer_5139 .

TY  - CONF
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5300
AB  - The bioremediation of heavy metals using microorganisms has received a great deal of attention for its potential application in industry. Among the most common heavy metals used and the more widespread contaminants of the environment is mercury, cadmium, lead and copper. The mechanisms associated with metal removal by microorganisms are complex and occur as: biosorption of metal ions on the cell surface, intracellular uptake of metal ions or chemical transformation of metal ions by microorganisms. Biosorption is a passive uptake of metals based upon physicochemical interactions between metal and functional groups of the cell wall. Intracellular metal uptake, sometimes called active biosorption is slow and nutrient dependent process. This way of uptake is accumulation of metal cations by living cells, where they were modified and used for microbial needs or in form that is less/no toxic, and returned in environment. Various microbial species, mainly Pseudomonas, have been shown to be efficient in removal of the different heavy metals from polluted effluents, using combinations of mentioned mechanisms. This study represents trial to characterize and determine Cd2+ uptake by strain P. aeruginosa san ai. In order to evaluate tolerance of the strain, MIC for cadmium were determined, afterwards the metal uptake was monitored. Preliminary results showed that MIC for Cd was 2 mM. More than 80% of cadmium added to the culture, grown in submerged conditions, was found to be associated with biomass. Investigation of distribution or metal in cell fractions, as well in protein, lipid and polysaccharide fractions is underway.
PB  - University of Münster
C3  - Programme - 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011
T1  - Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai
SP  - TBM12
UR  - https://hdl.handle.net/21.15107/rcub_cer_5300
ER  - 

@conference{
author = "Rikalović, Milena and Vrvić, Miroslav and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2011",
abstract = "The bioremediation of heavy metals using microorganisms has received a great deal of attention for its potential application in industry. Among the most common heavy metals used and the more widespread contaminants of the environment is mercury, cadmium, lead and copper. The mechanisms associated with metal removal by microorganisms are complex and occur as: biosorption of metal ions on the cell surface, intracellular uptake of metal ions or chemical transformation of metal ions by microorganisms. Biosorption is a passive uptake of metals based upon physicochemical interactions between metal and functional groups of the cell wall. Intracellular metal uptake, sometimes called active biosorption is slow and nutrient dependent process. This way of uptake is accumulation of metal cations by living cells, where they were modified and used for microbial needs or in form that is less/no toxic, and returned in environment. Various microbial species, mainly Pseudomonas, have been shown to be efficient in removal of the different heavy metals from polluted effluents, using combinations of mentioned mechanisms. This study represents trial to characterize and determine Cd2+ uptake by strain P. aeruginosa san ai. In order to evaluate tolerance of the strain, MIC for cadmium were determined, afterwards the metal uptake was monitored. Preliminary results showed that MIC for Cd was 2 mM. More than 80% of cadmium added to the culture, grown in submerged conditions, was found to be associated with biomass. Investigation of distribution or metal in cell fractions, as well in protein, lipid and polysaccharide fractions is underway.",
publisher = "University of Münster",
journal = "Programme - 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011",
title = "Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai",
pages = "TBM12",
url = "https://hdl.handle.net/21.15107/rcub_cer_5300"
}

Rikalović, M., Vrvić, M., Gojgić-Cvijović, G.,& Karadžić, I.. (2011). Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai. in Programme - 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011
University of Münster., TBM12.
https://hdl.handle.net/21.15107/rcub_cer_5300

Rikalović M, Vrvić M, Gojgić-Cvijović G, Karadžić I. Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai. in Programme - 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011. 2011;:TBM12.
https://hdl.handle.net/21.15107/rcub_cer_5300 .

Rikalović, Milena, Vrvić, Miroslav, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Uptake and distribution of cadmium by strain Pseudomonas aeruginosa san ai" in Programme - 3rd International Symposium on Metallomics "Metallomics 2011", June 15-18 2011, Münster, Germany, 2011 (2011):TBM12,
https://hdl.handle.net/21.15107/rcub_cer_5300 .

TY  - CONF
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5296
AB  - This study represents analysis of carbon sources effect on composition of rhamnolipid mixture produces by strain P aeruginosa NCAIM (P) B 001380 isolated from high alkaline mineral cutting oil. Strain was grown on PPAS medium supplemented with different carbon sources (2%), two simple (glucose and glucose with addition of kerosene) and two complexs, waste, sources (frying sunflower oil and sunflower oil mill effluent, SME). Isolated rhamnolipid mixtures were analyzed by HPLC ESI-MS. Results showed that retention times depend on lipidic component of rhamnolipid, not only on molecular weight.
PB  - Belgrade : Serbian Chemical Society
C3  - 16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia
T1  - Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS
SP  - MS17
UR  - https://hdl.handle.net/21.15107/rcub_cer_5296
ER  - 

@conference{
author = "Rikalović, Milena and Vrvić, Miroslav and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2011",
abstract = "This study represents analysis of carbon sources effect on composition of rhamnolipid mixture produces by strain P aeruginosa NCAIM (P) B 001380 isolated from high alkaline mineral cutting oil. Strain was grown on PPAS medium supplemented with different carbon sources (2%), two simple (glucose and glucose with addition of kerosene) and two complexs, waste, sources (frying sunflower oil and sunflower oil mill effluent, SME). Isolated rhamnolipid mixtures were analyzed by HPLC ESI-MS. Results showed that retention times depend on lipidic component of rhamnolipid, not only on molecular weight.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia",
title = "Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS",
pages = "MS17",
url = "https://hdl.handle.net/21.15107/rcub_cer_5296"
}

Rikalović, M., Vrvić, M., Gojgić-Cvijović, G.,& Karadžić, I.. (2011). Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS. in 16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia
Belgrade : Serbian Chemical Society., MS17.
https://hdl.handle.net/21.15107/rcub_cer_5296

Rikalović M, Vrvić M, Gojgić-Cvijović G, Karadžić I. Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS. in 16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia. 2011;:MS17.
https://hdl.handle.net/21.15107/rcub_cer_5296 .

Rikalović, Milena, Vrvić, Miroslav, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Comparative analysis of rhamnolipids produced by Pseudomonas aeruginosa NCAIM (P) B 001380 on different carbon sources by HPLC-ESI-MS" in 16th European Conference on Analytical Chemistry "Euroanalysis 2011", September 11-15, 2011, Belgrade, Serbia (2011):MS17,
https://hdl.handle.net/21.15107/rcub_cer_5296 .

TY  - CONF
AU  - Izrael-Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Natić, Maja
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5200
AB  - In the present study, the potential of bacterial lipase isolated from Pseudomonas aeruginosa NCATM (P) B 001380 to catalyze the esterification of ergosterol with oleic acid in the simple process, was evaluated. The prepared ergosterol oleate was purified by column chromatography and identification was conducted by NMR.
PB  - Beograd: Udruženje mikrobioologa Srbije
C3  - Proceedings - 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011
T1  - Pseudomonas lipase catalyzed synthesis of ergosterol oleate
SP  - pp. 2
UR  - https://hdl.handle.net/21.15107/rcub_cer_5200
ER  - 

@conference{
author = "Izrael-Živković, Lidija and Gojgić-Cvijović, Gordana and Natić, Maja and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2011",
abstract = "In the present study, the potential of bacterial lipase isolated from Pseudomonas aeruginosa NCATM (P) B 001380 to catalyze the esterification of ergosterol with oleic acid in the simple process, was evaluated. The prepared ergosterol oleate was purified by column chromatography and identification was conducted by NMR.",
publisher = "Beograd: Udruženje mikrobioologa Srbije",
journal = "Proceedings - 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011",
title = "Pseudomonas lipase catalyzed synthesis of ergosterol oleate",
pages = "pp. 2",
url = "https://hdl.handle.net/21.15107/rcub_cer_5200"
}

Izrael-Živković, L., Gojgić-Cvijović, G., Natić, M., Vrvić, M.,& Karadžić, I.. (2011). Pseudomonas lipase catalyzed synthesis of ergosterol oleate. in Proceedings - 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011
Beograd: Udruženje mikrobioologa Srbije., pp. 2.
https://hdl.handle.net/21.15107/rcub_cer_5200

Izrael-Živković L, Gojgić-Cvijović G, Natić M, Vrvić M, Karadžić I. Pseudomonas lipase catalyzed synthesis of ergosterol oleate. in Proceedings - 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011. 2011;:pp. 2.
https://hdl.handle.net/21.15107/rcub_cer_5200 .

Izrael-Živković, Lidija, Gojgić-Cvijović, Gordana, Natić, Maja, Vrvić, Miroslav, Karadžić, Ivanka, "Pseudomonas lipase catalyzed synthesis of ergosterol oleate" in Proceedings - 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, Belgrade, October 25-29, 2011 (2011):pp. 2,
https://hdl.handle.net/21.15107/rcub_cer_5200 .

TY  - CONF
AU  - Rikalović, Milena
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5138
PB  - Beograd: Udruženje mikrobioologa Srbije
C3  - CD-ROM Proceedings od the 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011)
T1  - MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380
SP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_cer_5138
ER  - 

@conference{
author = "Rikalović, Milena and Gojgić-Cvijović, Gordana and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2011",
publisher = "Beograd: Udruženje mikrobioologa Srbije",
journal = "CD-ROM Proceedings od the 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011)",
title = "MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380",
pages = "2",
url = "https://hdl.handle.net/21.15107/rcub_cer_5138"
}

Rikalović, M., Gojgić-Cvijović, G., Vrvić, M.,& Karadžić, I.. (2011). MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380. in CD-ROM Proceedings od the 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011)
Beograd: Udruženje mikrobioologa Srbije., 2.
https://hdl.handle.net/21.15107/rcub_cer_5138

Rikalović M, Gojgić-Cvijović G, Vrvić M, Karadžić I. MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380. in CD-ROM Proceedings od the 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011). 2011;:2.
https://hdl.handle.net/21.15107/rcub_cer_5138 .

Rikalović, Milena, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, Karadžić, Ivanka, "MALDI-TOF analysis of rhamnolipids produced by P. Aeruginosa NCAIM (P) B001380" in CD-ROM Proceedings od the 7th Balkan Congress of Microbiology and 8th Congress of Serbian Microbiologists, (Belgrade, October 25-29, 2011) (2011):2,
https://hdl.handle.net/21.15107/rcub_cer_5138 .

TY  - CONF
AU  - Izrael-Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5062
PB  - Belgrade : Serbian Chemical Society
C3  - Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)
T1  - Ispitivanja potencijala lipaze Pseudomonas aeruginosa san ai za sintezu estara u nevodenoj sredini
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_cer_5062
ER  - 

@conference{
author = "Izrael-Živković, Lidija and Gojgić-Cvijović, Gordana and Rikalović, Milena and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2011",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)",
title = "Ispitivanja potencijala lipaze Pseudomonas aeruginosa san ai za sintezu estara u nevodenoj sredini",
pages = "104",
url = "https://hdl.handle.net/21.15107/rcub_cer_5062"
}

Izrael-Živković, L., Gojgić-Cvijović, G., Rikalović, M., Vrvić, M.,& Karadžić, I.. (2011). Ispitivanja potencijala lipaze Pseudomonas aeruginosa san ai za sintezu estara u nevodenoj sredini. in Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)
Belgrade : Serbian Chemical Society., 104.
https://hdl.handle.net/21.15107/rcub_cer_5062

Izrael-Živković L, Gojgić-Cvijović G, Rikalović M, Vrvić M, Karadžić I. Ispitivanja potencijala lipaze Pseudomonas aeruginosa san ai za sintezu estara u nevodenoj sredini. in Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac). 2011;:104.
https://hdl.handle.net/21.15107/rcub_cer_5062 .

Izrael-Živković, Lidija, Gojgić-Cvijović, Gordana, Rikalović, Milena, Vrvić, Miroslav, Karadžić, Ivanka, "Ispitivanja potencijala lipaze Pseudomonas aeruginosa san ai za sintezu estara u nevodenoj sredini" in Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac) (2011):104,
https://hdl.handle.net/21.15107/rcub_cer_5062 .

TY  - CONF
AU  - Rikalović, Milena
AU  - Vrvić, Miroslav
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5055
AB  - Zagadenje zivotne okoline teskim metalima je ozbiljan problem savremenog sveta. Među metalima koji su cesti zagadjivaci su: bakar, kadmijum i ziva. Cilj ovog rada je bilo ispitivanje osetljivosti soja Pseudomonas aeruginosa san ai na pomenute teske metale. Minimale inhibitome koncentracije kadmijuma i bakra od 2 i 4 mM, redom, pokazale su da je soj otporan na ova dva metala. MIK za zivu je bila najniza testirana koncentracija metala od 0,5 mM. Dobijene vrednosti MIK-aza kadmijum i bakar stavljaju soj P. aeruginosa san ai u grupu bakterija koje ispoljavaju visokutoleranciju prema ovim teskim metalima. Utvrdeno je daje ziva toksicna za soj. lspitivanja
mehanizama detoksikacije soja P. aeruginosa san ai u cilju potencijalne primene u procesima
bioremedijacije su u toku.
AB  - The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. Among the common heavy metals used and the more widespread
contaminants of the environment are mercury, cadmium and copper. The present research was
performed in order to screen tolerance of strain Pseudomonas aeruginosa san ai to these heavy
metals Study showed that P. aeruginosa san ai is tolerant to cadmium and copper with MIC's of 2
and4 mM, respectively. MIC for mercury was the lowest tested concentration of 0.5 roM. This
suggests that P. aeruginosa san ai has a large potential for bioremediation of environments
contaminated with cadmium and copper and that mercury is toxic for the strain. In order to
improve possible application of strain, P. aeruginosa san ai in processes of bioremediation the
study of mechanisams of metal uptake are underway.
PB  - Belgrade : Serbian Chemical Society
C3  - Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)
T1  - Ispitivanje tolerancije soja Pseudomonas aeruginosa san ai na živu, kadmijum i bakar
T1  - Study of tolerance of Pseudomonas aeruginosa san ai to mercury, cadmium and copper
SP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_cer_5055
ER  - 

@conference{
author = "Rikalović, Milena and Vrvić, Miroslav and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2011",
abstract = "Zagadenje zivotne okoline teskim metalima je ozbiljan problem savremenog sveta. Među metalima koji su cesti zagadjivaci su: bakar, kadmijum i ziva. Cilj ovog rada je bilo ispitivanje osetljivosti soja Pseudomonas aeruginosa san ai na pomenute teske metale. Minimale inhibitome koncentracije kadmijuma i bakra od 2 i 4 mM, redom, pokazale su da je soj otporan na ova dva metala. MIK za zivu je bila najniza testirana koncentracija metala od 0,5 mM. Dobijene vrednosti MIK-aza kadmijum i bakar stavljaju soj P. aeruginosa san ai u grupu bakterija koje ispoljavaju visokutoleranciju prema ovim teskim metalima. Utvrdeno je daje ziva toksicna za soj. lspitivanja
mehanizama detoksikacije soja P. aeruginosa san ai u cilju potencijalne primene u procesima
bioremedijacije su u toku., The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. Among the common heavy metals used and the more widespread
contaminants of the environment are mercury, cadmium and copper. The present research was
performed in order to screen tolerance of strain Pseudomonas aeruginosa san ai to these heavy
metals Study showed that P. aeruginosa san ai is tolerant to cadmium and copper with MIC's of 2
and4 mM, respectively. MIC for mercury was the lowest tested concentration of 0.5 roM. This
suggests that P. aeruginosa san ai has a large potential for bioremediation of environments
contaminated with cadmium and copper and that mercury is toxic for the strain. In order to
improve possible application of strain, P. aeruginosa san ai in processes of bioremediation the
study of mechanisams of metal uptake are underway.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)",
title = "Ispitivanje tolerancije soja Pseudomonas aeruginosa san ai na živu, kadmijum i bakar, Study of tolerance of Pseudomonas aeruginosa san ai to mercury, cadmium and copper",
pages = "99",
url = "https://hdl.handle.net/21.15107/rcub_cer_5055"
}

Rikalović, M., Vrvić, M., Gojgić-Cvijović, G.,& Karadžić, I.. (2011). Ispitivanje tolerancije soja Pseudomonas aeruginosa san ai na živu, kadmijum i bakar. in Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)
Belgrade : Serbian Chemical Society., 99.
https://hdl.handle.net/21.15107/rcub_cer_5055

Rikalović M, Vrvić M, Gojgić-Cvijović G, Karadžić I. Ispitivanje tolerancije soja Pseudomonas aeruginosa san ai na živu, kadmijum i bakar. in Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac). 2011;:99.
https://hdl.handle.net/21.15107/rcub_cer_5055 .

Rikalović, Milena, Vrvić, Miroslav, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Ispitivanje tolerancije soja Pseudomonas aeruginosa san ai na živu, kadmijum i bakar" in Program i kratki izvodi radova XLIX Savetovanja Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac) (2011):99,
https://hdl.handle.net/21.15107/rcub_cer_5055 .

TY  - JOUR
AU  - Izrael‑Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/713
AB  - Enzymatic characteristics of a protease from a medically important, referent strain of Pseudomonas aeruginosa ATCC 27853 were determined. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE, and gel filtration, it was estimated that the molecular mass of the purified enzyme was about 15 kDa. Other enzymatic properties were found to be: pH optimum 7.1, pH stability between 6.5 and 10; temperature optimum around 60 °C while the enzyme was stable at 60°C for 30 min. Inhibition of the enzyme was observed with metal chelators, such as EDTA and 1,10-phenanthroline, suggesting that the protease is a metalloenzyme. Furthermore, the enzyme contains one mole of zinc ion per mole of enzyme. The protease was stable in the presence of different organic solvents, which enables its potential use for the synthesis of peptides.
AB  - U ovom radu je okarakterisana ekstracelularna proteaza medicinski značajnog, referentnog soja Pseudomonas aeruginosa ATCC 27853. Molekulska masa prečišćenog enzima određena SDS PAGE i gel filtracijom iznosi oko 15 kDa. Određeni su sledeći enzimski parametri: pH optimum 7,1; pH stabilnost u opsegu 6,5-10; temperaturni optimum 60°C, a enzim je stabilan na 60°C 30 min. Na osnovu inhibicije enzima pomoću EDTA i 1,10-fenantrolina, utvrđeno je da proteaza predstavlja metaloenzim. Pokazano da proteaza sadrži 1 mol jona cinka po molu enzima. Proteaza je stabilna u prisustvu različitih organskih rastvarača, što omogućava upotrebu za sintezu peptida.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853
T1  - Izolovanje i delimično karakterisanje proteaze iz Pseudomonas aeruginosa ATCC 27853
VL  - 75
IS  - 8
SP  - 1041
EP  - 1052
DO  - 10.2298/JSC100125088I
ER  - 

@article{
author = "Izrael‑Živković, Lidija and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2010",
abstract = "Enzymatic characteristics of a protease from a medically important, referent strain of Pseudomonas aeruginosa ATCC 27853 were determined. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE, and gel filtration, it was estimated that the molecular mass of the purified enzyme was about 15 kDa. Other enzymatic properties were found to be: pH optimum 7.1, pH stability between 6.5 and 10; temperature optimum around 60 °C while the enzyme was stable at 60°C for 30 min. Inhibition of the enzyme was observed with metal chelators, such as EDTA and 1,10-phenanthroline, suggesting that the protease is a metalloenzyme. Furthermore, the enzyme contains one mole of zinc ion per mole of enzyme. The protease was stable in the presence of different organic solvents, which enables its potential use for the synthesis of peptides., U ovom radu je okarakterisana ekstracelularna proteaza medicinski značajnog, referentnog soja Pseudomonas aeruginosa ATCC 27853. Molekulska masa prečišćenog enzima određena SDS PAGE i gel filtracijom iznosi oko 15 kDa. Određeni su sledeći enzimski parametri: pH optimum 7,1; pH stabilnost u opsegu 6,5-10; temperaturni optimum 60°C, a enzim je stabilan na 60°C 30 min. Na osnovu inhibicije enzima pomoću EDTA i 1,10-fenantrolina, utvrđeno je da proteaza predstavlja metaloenzim. Pokazano da proteaza sadrži 1 mol jona cinka po molu enzima. Proteaza je stabilna u prisustvu različitih organskih rastvarača, što omogućava upotrebu za sintezu peptida.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853, Izolovanje i delimično karakterisanje proteaze iz Pseudomonas aeruginosa ATCC 27853",
volume = "75",
number = "8",
pages = "1041-1052",
doi = "10.2298/JSC100125088I"
}

Izrael‑Živković, L., Gojgić-Cvijović, G.,& Karadžić, I.. (2010). Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 75(8), 1041-1052.
https://doi.org/10.2298/JSC100125088I

Izrael‑Živković L, Gojgić-Cvijović G, Karadžić I. Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. in Journal of the Serbian Chemical Society. 2010;75(8):1041-1052.
doi:10.2298/JSC100125088I .

Izrael‑Živković, Lidija, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853" in Journal of the Serbian Chemical Society, 75, no. 8 (2010):1041-1052,
https://doi.org/10.2298/JSC100125088I . .

TY  - JOUR
AU  - Izrael‑Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Gopcevic, K.R.
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/587
AB  - An extracellular lipase from Pseudomonas aeruginosa ATCC 27853 has been purified and its enzymatic characteristics were determined. According to SDS-PAGE and gel filtration molecular mass estimated to be 30 kDa, what classified the lipase in group I.1. Although 14 lipases from P. aeruginosa with similar molecular mass are referred to date, their basic enzymatic properties have not been reported yet. To address the gap we found: the optimal temperature and pH in water solution being 50 °C and 9.3, respectively; the lipase was inhibited with Hg2+ ions and sodium dodecylsulphate (SDS), while non-ionic detergent Triton X-100 activated the enzyme; the lipase hydrolyzed more rapidly middle chain triglycerides and it was not regiospecific; the lipase demonstrated naturally occurring stability in different organic solvents with concentrations ranging from 30 to 70%, including good thermal stability in 30% organic solvent solution. Even though strain P. aeruginosa ATCC 27853 was not isolated from extreme environment it showed activity in organic solvent suggesting that this lipase is suitable for variety of applications, including reactions in water restricted medium and bioremediation of contaminations by organic solvents.
T2  - Journal of Basic Microbiology
T1  - Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853
VL  - 49
IS  - 5
SP  - 452
EP  - 462
DO  - 10.1002/jobm.200800229
ER  - 

@article{
author = "Izrael‑Živković, Lidija and Gojgić-Cvijović, Gordana and Gopcevic, K.R. and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2009",
abstract = "An extracellular lipase from Pseudomonas aeruginosa ATCC 27853 has been purified and its enzymatic characteristics were determined. According to SDS-PAGE and gel filtration molecular mass estimated to be 30 kDa, what classified the lipase in group I.1. Although 14 lipases from P. aeruginosa with similar molecular mass are referred to date, their basic enzymatic properties have not been reported yet. To address the gap we found: the optimal temperature and pH in water solution being 50 °C and 9.3, respectively; the lipase was inhibited with Hg2+ ions and sodium dodecylsulphate (SDS), while non-ionic detergent Triton X-100 activated the enzyme; the lipase hydrolyzed more rapidly middle chain triglycerides and it was not regiospecific; the lipase demonstrated naturally occurring stability in different organic solvents with concentrations ranging from 30 to 70%, including good thermal stability in 30% organic solvent solution. Even though strain P. aeruginosa ATCC 27853 was not isolated from extreme environment it showed activity in organic solvent suggesting that this lipase is suitable for variety of applications, including reactions in water restricted medium and bioremediation of contaminations by organic solvents.",
journal = "Journal of Basic Microbiology",
title = "Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853",
volume = "49",
number = "5",
pages = "452-462",
doi = "10.1002/jobm.200800229"
}

Izrael‑Živković, L., Gojgić-Cvijović, G., Gopcevic, K.R., Vrvić, M.,& Karadžić, I.. (2009). Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853. in Journal of Basic Microbiology, 49(5), 452-462.
https://doi.org/10.1002/jobm.200800229

Izrael‑Živković L, Gojgić-Cvijović G, Gopcevic K, Vrvić M, Karadžić I. Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853. in Journal of Basic Microbiology. 2009;49(5):452-462.
doi:10.1002/jobm.200800229 .

Izrael‑Živković, Lidija, Gojgić-Cvijović, Gordana, Gopcevic, K.R., Vrvić, Miroslav, Karadžić, Ivanka, "Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853" in Journal of Basic Microbiology, 49, no. 5 (2009):452-462,
https://doi.org/10.1002/jobm.200800229 . .

TY  - JOUR
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
AU  - Gopčević, Kristina
AU  - Borozan, Sunčica
PY  - 2006
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/228
AB  - Streptomycetes are common soil bacteria that grow in a filamentous form and produced spores. Secondary metabolites, particularly antibiotics, as well as extracellular enzymes produced by them have very significant role in the process of differentiation. The Slreptomyces hygroscopicus CH-7 strain produces polyketide antibiotics and an extracellular proteolytic complex. The aim of this work was to establish the influence of transcription and translation inhibitors on biosynthesis of polyene antibiotic and proteolytic enzymes. The inhibitors were added at inoculation and at specific time intervals of fermentation. The production of hexaene H-85 is susceptible to rifamycin (transcription inhibitor) and chloramphenicol (translation inhibitor). Rifamycin reduces the production and trypsin like protease (TLP) and aminopeptidase (AP). while chloramphenicol acts only on TLP. Simultaneously these inhibitors almost have no effect on strain growth.
AB  - Streptomicete su gram pozitivne bakterije čije je najvažnije stanište zemlja. Pri rastu na čvrstim podlogama i u submerznim uslovima grade supstratni i vazdušni micelijum. Morfološka diferencijacija je praćena produkcijom sekundarnih metabolita kao što su antibiotici, a i različitih hidrolitičkih enzima. Za mikroorganizme koji ih proizvode antibiotici predstavljaju kompetitivno oružje, agense za transport metala kao i efektore diferencijacije. Proteolitički enzimi, takođe, imaju određenu ulogu u razvojnom ciklusu aktinomiceta. Na primer, uključeni su u procese građenja vazdušnog micelija i sporulaciju. Soj Streptomyces hygroscopicus CH-7 pri fermentaciji proizvodi antibiotike poliketidne strukture i smesu ekstracelularnih proteolitičkih enzima. U ovom radu je ispitivan uticaj antibiotika rifamicina i hloramfenikola, inhibitora transkripcije i translacije, na produkciju polienskog antibiotika heksaena H-85 i proteolitičkih enzima. Inhibited su dodavani pri inokulaciji (O h) i u različitim vremenskim intervalima fermentacije (12-48 h). Dejstvom antibiotika inhibitora transkripcije i translacije utvrdjeno je da se enzim polien sintetaza transkribuje do 12. časa, a translacija teče do 48. časa fermentacije. Dodatak rifamicina pri zasejavanju drastično snižava proteolitičku aktivnost, a u kasnijim fazama izaziva smanjenje (36-80%). Hloramfenikol smanjuje produkciju tripsinu slične proteaze. ali nema dejstvo na aminopeptidaznu aktivnost. Rifamicin i hloramfenikol ne pokazuju značajno inhibitorno dejstvo na rast soja.
T2  - Acta biologica iugoslavica - serija B: Mikrobiologija
T1  - The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7
T1  - Dejstvo rifamicina i hloramfenikola na produkciju antibiotika i proteaza soja Streptomyces hygroscopicus CH-7
VL  - 43
IS  - 1
SP  - 31
EP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_cer_228
ER  - 

@article{
author = "Gojgić-Cvijović, Gordana and Karadžić, Ivanka and Gopčević, Kristina and Borozan, Sunčica",
year = "2006",
abstract = "Streptomycetes are common soil bacteria that grow in a filamentous form and produced spores. Secondary metabolites, particularly antibiotics, as well as extracellular enzymes produced by them have very significant role in the process of differentiation. The Slreptomyces hygroscopicus CH-7 strain produces polyketide antibiotics and an extracellular proteolytic complex. The aim of this work was to establish the influence of transcription and translation inhibitors on biosynthesis of polyene antibiotic and proteolytic enzymes. The inhibitors were added at inoculation and at specific time intervals of fermentation. The production of hexaene H-85 is susceptible to rifamycin (transcription inhibitor) and chloramphenicol (translation inhibitor). Rifamycin reduces the production and trypsin like protease (TLP) and aminopeptidase (AP). while chloramphenicol acts only on TLP. Simultaneously these inhibitors almost have no effect on strain growth., Streptomicete su gram pozitivne bakterije čije je najvažnije stanište zemlja. Pri rastu na čvrstim podlogama i u submerznim uslovima grade supstratni i vazdušni micelijum. Morfološka diferencijacija je praćena produkcijom sekundarnih metabolita kao što su antibiotici, a i različitih hidrolitičkih enzima. Za mikroorganizme koji ih proizvode antibiotici predstavljaju kompetitivno oružje, agense za transport metala kao i efektore diferencijacije. Proteolitički enzimi, takođe, imaju određenu ulogu u razvojnom ciklusu aktinomiceta. Na primer, uključeni su u procese građenja vazdušnog micelija i sporulaciju. Soj Streptomyces hygroscopicus CH-7 pri fermentaciji proizvodi antibiotike poliketidne strukture i smesu ekstracelularnih proteolitičkih enzima. U ovom radu je ispitivan uticaj antibiotika rifamicina i hloramfenikola, inhibitora transkripcije i translacije, na produkciju polienskog antibiotika heksaena H-85 i proteolitičkih enzima. Inhibited su dodavani pri inokulaciji (O h) i u različitim vremenskim intervalima fermentacije (12-48 h). Dejstvom antibiotika inhibitora transkripcije i translacije utvrdjeno je da se enzim polien sintetaza transkribuje do 12. časa, a translacija teče do 48. časa fermentacije. Dodatak rifamicina pri zasejavanju drastično snižava proteolitičku aktivnost, a u kasnijim fazama izaziva smanjenje (36-80%). Hloramfenikol smanjuje produkciju tripsinu slične proteaze. ali nema dejstvo na aminopeptidaznu aktivnost. Rifamicin i hloramfenikol ne pokazuju značajno inhibitorno dejstvo na rast soja.",
journal = "Acta biologica iugoslavica - serija B: Mikrobiologija",
title = "The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7, Dejstvo rifamicina i hloramfenikola na produkciju antibiotika i proteaza soja Streptomyces hygroscopicus CH-7",
volume = "43",
number = "1",
pages = "31-40",
url = "https://hdl.handle.net/21.15107/rcub_cer_228"
}

Gojgić-Cvijović, G., Karadžić, I., Gopčević, K.,& Borozan, S.. (2006). The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7. in Acta biologica iugoslavica - serija B: Mikrobiologija, 43(1), 31-40.
https://hdl.handle.net/21.15107/rcub_cer_228

Gojgić-Cvijović G, Karadžić I, Gopčević K, Borozan S. The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7. in Acta biologica iugoslavica - serija B: Mikrobiologija. 2006;43(1):31-40.
https://hdl.handle.net/21.15107/rcub_cer_228 .

Gojgić-Cvijović, Gordana, Karadžić, Ivanka, Gopčević, Kristina, Borozan, Sunčica, "The effect of rifamycin and chloramphenicol on antibiotic and protease production by Streptomyces hygroscopicus CH-7" in Acta biologica iugoslavica - serija B: Mikrobiologija, 43, no. 1 (2006):31-40,
https://hdl.handle.net/21.15107/rcub_cer_228 .