TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Mišić, Milan
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5787
AB  - Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.
PB  - Elsevier Ltd
T2  - Fuel
T1  - Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch
VL  - 338
SP  - 127363
DO  - 10.1016/j.fuel.2022.127363
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Mišić, Milan and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.",
publisher = "Elsevier Ltd",
journal = "Fuel",
title = "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch",
volume = "338",
pages = "127363",
doi = "10.1016/j.fuel.2022.127363"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel
Elsevier Ltd., 338, 127363.
https://doi.org/10.1016/j.fuel.2022.127363

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Mišić M, Božić N, Vujčić Z. Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel. 2023;338:127363.
doi:10.1016/j.fuel.2022.127363 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Mišić, Milan, Božić, Nataša, Vujčić, Zoran, "Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch" in Fuel, 338 (2023):127363,
https://doi.org/10.1016/j.fuel.2022.127363 . .

TY  - CONF
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6029
AB  - Since each region of the world has different agricultural sectors, specific residues are generating and at the same time a wide range of alternative novel sources of nutrients emerge, such as carbohydrates, proteins and minerals. Usage of biomass residues as start material for prebiotics production is preferable from the standpoint of ecology and as cheap production process. Corn cob can be considered as the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, with biological benefits such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity. The European Commission has approved XOS produced from corn cob as a safe for human consumption. The corn cob mainly consists of lignocellulose. Chemical treatment is used to extract the hemicelullose from corn cob which is then hydrolyzed to XOS by fungal xylanases. Xylanase produced by fungi genera Aspergillus and Trichoderma are considered key enzymes for XOS production. Fermentation enzyme extract of strain Aspergillus tubingensis FAT35, obtained after SSF on corn cob as substrate, was used for hydrolysis of corn cob xylan to XOS. A. tubigensis FAT35 produced high level of xylanase (350 U/mL). Obtained XOS (2-10 units) were characterized by TLC and by antioxidant activity (ORAC, DPPH, FRAP, and ABTS). Significant antioxidant potential was shown by all used antioxidant tests. The obtained XOS are suitable to be a functional food additive and are obtained from agro-waste material by environmentally and economically suitable way.
PB  - European Federation of Biotechnology
C3  - Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
T1  - Corn cob agro-waste as valuable material for XOS production by fungal enzymes
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_cer_6029
ER  - 

@conference{
author = "Ristović, Marina and Stojanović, Sanja and Šokarda Slavić, Marinela and Margetić, Aleksandra and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Since each region of the world has different agricultural sectors, specific residues are generating and at the same time a wide range of alternative novel sources of nutrients emerge, such as carbohydrates, proteins and minerals. Usage of biomass residues as start material for prebiotics production is preferable from the standpoint of ecology and as cheap production process. Corn cob can be considered as the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, with biological benefits such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity. The European Commission has approved XOS produced from corn cob as a safe for human consumption. The corn cob mainly consists of lignocellulose. Chemical treatment is used to extract the hemicelullose from corn cob which is then hydrolyzed to XOS by fungal xylanases. Xylanase produced by fungi genera Aspergillus and Trichoderma are considered key enzymes for XOS production. Fermentation enzyme extract of strain Aspergillus tubingensis FAT35, obtained after SSF on corn cob as substrate, was used for hydrolysis of corn cob xylan to XOS. A. tubigensis FAT35 produced high level of xylanase (350 U/mL). Obtained XOS (2-10 units) were characterized by TLC and by antioxidant activity (ORAC, DPPH, FRAP, and ABTS). Significant antioxidant potential was shown by all used antioxidant tests. The obtained XOS are suitable to be a functional food additive and are obtained from agro-waste material by environmentally and economically suitable way.",
publisher = "European Federation of Biotechnology",
journal = "Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online",
title = "Corn cob agro-waste as valuable material for XOS production by fungal enzymes",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_cer_6029"
}

Ristović, M., Stojanović, S., Šokarda Slavić, M., Margetić, A., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). Corn cob agro-waste as valuable material for XOS production by fungal enzymes. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
European Federation of Biotechnology., 67-67.
https://hdl.handle.net/21.15107/rcub_cer_6029

Ristović M, Stojanović S, Šokarda Slavić M, Margetić A, Božić N, Vujčić Z, Dojnov B. Corn cob agro-waste as valuable material for XOS production by fungal enzymes. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online. 2023;:67-67.
https://hdl.handle.net/21.15107/rcub_cer_6029 .

Ristović, Marina, Stojanović, Sanja, Šokarda Slavić, Marinela, Margetić, Aleksandra, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "Corn cob agro-waste as valuable material for XOS production by fungal enzymes" in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online (2023):67-67,
https://hdl.handle.net/21.15107/rcub_cer_6029 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6242
AB  - Most of the functional oligosaccharides (OS) consist of monomers, present in varying degrees of polymerization (DP) ranging between 3 and 10 units. DP of inulin-type fructooligosaccharides has a great impact on fermentability and their
utilization by probiotic bacteria such is Bifidobacteria, thus they have a great impact on their health-promoting effect. Technological properties of fructooligosaccharides (FOS) can improve the physicochemical and sensory characteristics of food products, leading to their increased application in the food industry. It has been found that microbial endoinulinase plays an important role in production of inulin-type fructooligosaccharides. Aspergillus welwitschiae FAW1 strain has proven to be non-toxigenic with the absence of biosynthetic gene clusters for mycotoxins (ochratoxins and fumonisins) and therefore safe for use in food production [5]. Growing on the natural substrate, triticale (Triticosecale sp) FAW1 strain produced inulinase complex from which endoinulinase (InuA) was purified by chromatographic techniques. FOS was prepared by time-controlled hydrolysis of inulin. Monitoring kinetics and determining the amount of obtained FOS by TLC and HPLC methods led to a conclusion that FOS production by hydrolysis of inulin is kinetic dependent reaction. Depending on the reaction time, FOS with different compositions are obtained.The largest amount of produced FOS (DP 2-6) has been in 15-20 minutes of the reaction, where the resulting mixture contains small amount of mono- and disaccharides. The obtained FOS were characterized on antioxidant capacity. Produced FOS showed significant antioxidant potential according to ORAC method which classifies them as potent candidate as additives in functional food. Endoinulinase (InuA) form A. welvitscihae FAW1 considered as key enzyme in FOS preparation. The composition and lenght of the produced FOS can be varied by controlling the reaction time, depending on the needs of of the market and their eventual application.
PB  - Serbian Chemical Society
C3  - Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase
SP  - 203
EP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_cer_6242
ER  - 

@conference{
author = "Stojanović, Sanja and Margetić, Aleksandra and Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Most of the functional oligosaccharides (OS) consist of monomers, present in varying degrees of polymerization (DP) ranging between 3 and 10 units. DP of inulin-type fructooligosaccharides has a great impact on fermentability and their
utilization by probiotic bacteria such is Bifidobacteria, thus they have a great impact on their health-promoting effect. Technological properties of fructooligosaccharides (FOS) can improve the physicochemical and sensory characteristics of food products, leading to their increased application in the food industry. It has been found that microbial endoinulinase plays an important role in production of inulin-type fructooligosaccharides. Aspergillus welwitschiae FAW1 strain has proven to be non-toxigenic with the absence of biosynthetic gene clusters for mycotoxins (ochratoxins and fumonisins) and therefore safe for use in food production [5]. Growing on the natural substrate, triticale (Triticosecale sp) FAW1 strain produced inulinase complex from which endoinulinase (InuA) was purified by chromatographic techniques. FOS was prepared by time-controlled hydrolysis of inulin. Monitoring kinetics and determining the amount of obtained FOS by TLC and HPLC methods led to a conclusion that FOS production by hydrolysis of inulin is kinetic dependent reaction. Depending on the reaction time, FOS with different compositions are obtained.The largest amount of produced FOS (DP 2-6) has been in 15-20 minutes of the reaction, where the resulting mixture contains small amount of mono- and disaccharides. The obtained FOS were characterized on antioxidant capacity. Produced FOS showed significant antioxidant potential according to ORAC method which classifies them as potent candidate as additives in functional food. Endoinulinase (InuA) form A. welvitscihae FAW1 considered as key enzyme in FOS preparation. The composition and lenght of the produced FOS can be varied by controlling the reaction time, depending on the needs of of the market and their eventual application.",
publisher = "Serbian Chemical Society",
journal = "Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase",
pages = "203-203",
url = "https://hdl.handle.net/21.15107/rcub_cer_6242"
}

Stojanović, S., Margetić, A., Šokarda Slavić, M., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Serbian Chemical Society., 203-203.
https://hdl.handle.net/21.15107/rcub_cer_6242

Stojanović S, Margetić A, Šokarda Slavić M, Božić N, Vujčić Z, Dojnov B. Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:203-203.
https://hdl.handle.net/21.15107/rcub_cer_6242 .

Stojanović, Sanja, Margetić, Aleksandra, Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "Obtaining of FOS by controlled hydrolysis of inulin with Aspergillus welwitschiae FAW1 endoinulinase" in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):203-203,
https://hdl.handle.net/21.15107/rcub_cer_6242 .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Stanisavljević, Nemanja
AU  - Gardijan, Lazar
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6458
AB  - α-Amylase from the thermophilic bacterial strain Anoxybacillus vranjensis ST4 (AVA) was cloned into the pMALc5HisEk expression vector and successfully expressed and purified from the Escherichia coli ER2523 host strain. AVA belongs to the GH13_5 subfamily of glycoside hydrolases and has 7 conserved sequence regions (CSRs) distributed in three distinct domains (A, B, C). In addition, there is a starch binding domain (SBD) from the CBM20 family of carbohydrate binding modules (CBMs). AVA is a monomer of 66 kDa that achieves maximum activity at 60–80 °C and is active and stable over a wide pH range (4.0–9.0). AVA retained 50 % of its activity after 31 h of incubation at 60 °C and was resistant to a large number of denaturing agents. It hydrolyzed starch granules very efficiently, releasing maltose, maltotriose and maltopentaose as the main products. The hydrolysis rates of raw corn, wheat, horseradish, and potato starch, at a concentration of 10 %, were 87.8, 85.9, 93.0, and 58 %, respectively, at pH 8.5 over a 3 h period. This study showed that the high level of expression as well as the properties of this highly stable and versatile enzyme show all the prerequisites for successful application in industry.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications
VL  - 249
SP  - 126055
DO  - 10.1016/j.ijbiomac.2023.126055
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Kojić, Milan and Margetić, Aleksandra and Stanisavljević, Nemanja and Gardijan, Lazar and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "α-Amylase from the thermophilic bacterial strain Anoxybacillus vranjensis ST4 (AVA) was cloned into the pMALc5HisEk expression vector and successfully expressed and purified from the Escherichia coli ER2523 host strain. AVA belongs to the GH13_5 subfamily of glycoside hydrolases and has 7 conserved sequence regions (CSRs) distributed in three distinct domains (A, B, C). In addition, there is a starch binding domain (SBD) from the CBM20 family of carbohydrate binding modules (CBMs). AVA is a monomer of 66 kDa that achieves maximum activity at 60–80 °C and is active and stable over a wide pH range (4.0–9.0). AVA retained 50 % of its activity after 31 h of incubation at 60 °C and was resistant to a large number of denaturing agents. It hydrolyzed starch granules very efficiently, releasing maltose, maltotriose and maltopentaose as the main products. The hydrolysis rates of raw corn, wheat, horseradish, and potato starch, at a concentration of 10 %, were 87.8, 85.9, 93.0, and 58 %, respectively, at pH 8.5 over a 3 h period. This study showed that the high level of expression as well as the properties of this highly stable and versatile enzyme show all the prerequisites for successful application in industry.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications",
volume = "249",
pages = "126055",
doi = "10.1016/j.ijbiomac.2023.126055"
}

Šokarda Slavić, M., Kojić, M., Margetić, A., Stanisavljević, N., Gardijan, L., Božić, N.,& Vujčić, Z.. (2023). Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications. in International Journal of Biological Macromolecules
Elsevier., 249, 126055.
https://doi.org/10.1016/j.ijbiomac.2023.126055

Šokarda Slavić M, Kojić M, Margetić A, Stanisavljević N, Gardijan L, Božić N, Vujčić Z. Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications. in International Journal of Biological Macromolecules. 2023;249:126055.
doi:10.1016/j.ijbiomac.2023.126055 .

Šokarda Slavić, Marinela, Kojić, Milan, Margetić, Aleksandra, Stanisavljević, Nemanja, Gardijan, Lazar, Božić, Nataša, Vujčić, Zoran, "Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications" in International Journal of Biological Macromolecules, 249 (2023):126055,
https://doi.org/10.1016/j.ijbiomac.2023.126055 . .

TY  - CONF
AU  - Ristović, Marina
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Šokarda Slavić, Marinela
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7135
AB  - Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitation from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process.
PB  - Elsevier
C3  - Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany
T1  - The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production
SP  - P2.002
UR  - https://hdl.handle.net/21.15107/rcub_cer_7135
ER  - 

@conference{
author = "Ristović, Marina and Margetić, Aleksandra and Stojanović, Sanja and Šokarda Slavić, Marinela and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Corn is widely used in the diet all over the world which generate corn cob as one of the most common agrowaste material. Corn cob mainly consists of lignocellulose with a high quantity of xylan; therefore, it can be considered the main source for xylooligosaccharides (XOS) production. XOS are prebiotic, functional food ingredients, which stand out on the market of oligosaccharide prebiotics because of their properties (antitumor effect, immunomodulatory effect, antioxidant power, and high pH and thermal stability). The European Commission has approved XOS produced from corn cob as safe for human consumption. For XOS production highly efficient xylanase obtained by Aspergillus tubingensis FAT35 under SSF on corn cob medium was used. Three methods of XOS production were evaluated: by hydrolysis of xylan alkali isolated from corn cob, by direct hydrolysis of xylan in corn cob and simultaneously production during fungal growth on corn cob (in situ). XOS were separated by ethanol precipitation from corn cob hydrolysate and from fermentation extract. In this way, the enzyme is concentrated in the precipitate, and XOS (remaining in the supernatant) are partially purified (separated from unhydrolyzed xylan and long chain oligomers). All prepared XOS were characterized by TLC and HPLC and antioxidant potential. Thanks to the high antioxidant potential (proven by DPPH, ABTS, FRAP, and ORAC tests) obtained XOS are good candidates for a functional food additive. Although the highest yield of XOS was obtained by the standard method (enzymatic hydrolysis of isolated xylan), additional methods represent the direct use of agro-waste material in an environmentally and economically suitable way. Special attention attracts in situ production because after isolation of XOS by ethanol precipitation the enzyme remains functional active and available for further use in next process.",
publisher = "Elsevier",
journal = "Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany",
title = "The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production",
pages = "P2.002",
url = "https://hdl.handle.net/21.15107/rcub_cer_7135"
}

Ristović, M., Margetić, A., Stojanović, S., Šokarda Slavić, M., Božić, N., Vujčić, Z.,& Dojnov, B.. (2023). The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production. in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany
Elsevier., P2.002.
https://hdl.handle.net/21.15107/rcub_cer_7135

Ristović M, Margetić A, Stojanović S, Šokarda Slavić M, Božić N, Vujčić Z, Dojnov B. The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production. in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany. 2023;:P2.002.
https://hdl.handle.net/21.15107/rcub_cer_7135 .

Ristović, Marina, Margetić, Aleksandra, Stojanović, Sanja, Šokarda Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, "The use of corn cob as an excellent starting material for obtaining XOS and fungal enzymes involved in their production" in Book of Abstracts - 3rd Food Chemistry Conference, 10-12 October 2023, Dresden, Germany (2023):P2.002,
https://hdl.handle.net/21.15107/rcub_cer_7135 .

TY  - CONF
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Ristović, Marina
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7137
AB  - Given the growing concern about the depletion of fossil fuels, global warming, and the loss of natural resources, bioethanol made from sugar cane, molasses, and corn continues to garner interest globally and is regarded as the safest and cleanest alternative to oil. Starch is a widely available renewable carbohydrate from which bioethanol is conventionally obtained through energy demanding liquefaction and saccharification processes. A significant simplification of the process and a reduction of starch processing costs would be possible by applying raw starch hydrolysis using enzymes capable of degrading starch below the gelatinization temperature. A novel strategy for highly concentrated raw corn starch (30 % w/v) hydrolysis based on a modified simultaneous saccharification and fermentation process is optimized for the production of bioethanol. Different ratios of Bacillus paralicheniformis ATCC 9945a (BliAmy) and glucoamylase (Dextrozyme® GA), glucoamylase addition time, incubation time, and pH were investigated using a Box–Behnken experimental design to ensure high process efficiency. A two-step synergistic hydrolysis and fermentation with Saccharomyces cerevisiae at 30 °C was carried out in a single bioreactor vessel at the same pH (4.5). The obtained bioethanol concentration at 129.2 g/L, with a productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield indicates the viability of the proposed innovative process.
PB  - Slovenian Biochemical Society
C3  - Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia
T1  - An innovative process for the production of bioethanol: Optimization and kinetic assessment
SP  - P59
UR  - https://hdl.handle.net/21.15107/rcub_cer_7137
ER  - 

@conference{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Ristović, Marina and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Given the growing concern about the depletion of fossil fuels, global warming, and the loss of natural resources, bioethanol made from sugar cane, molasses, and corn continues to garner interest globally and is regarded as the safest and cleanest alternative to oil. Starch is a widely available renewable carbohydrate from which bioethanol is conventionally obtained through energy demanding liquefaction and saccharification processes. A significant simplification of the process and a reduction of starch processing costs would be possible by applying raw starch hydrolysis using enzymes capable of degrading starch below the gelatinization temperature. A novel strategy for highly concentrated raw corn starch (30 % w/v) hydrolysis based on a modified simultaneous saccharification and fermentation process is optimized for the production of bioethanol. Different ratios of Bacillus paralicheniformis ATCC 9945a (BliAmy) and glucoamylase (Dextrozyme® GA), glucoamylase addition time, incubation time, and pH were investigated using a Box–Behnken experimental design to ensure high process efficiency. A two-step synergistic hydrolysis and fermentation with Saccharomyces cerevisiae at 30 °C was carried out in a single bioreactor vessel at the same pH (4.5). The obtained bioethanol concentration at 129.2 g/L, with a productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield indicates the viability of the proposed innovative process.",
publisher = "Slovenian Biochemical Society",
journal = "Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia",
title = "An innovative process for the production of bioethanol: Optimization and kinetic assessment",
pages = "P59",
url = "https://hdl.handle.net/21.15107/rcub_cer_7137"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Ristović, M., Božić, N.,& Vujčić, Z.. (2023). An innovative process for the production of bioethanol: Optimization and kinetic assessment. in Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia
Slovenian Biochemical Society., P59.
https://hdl.handle.net/21.15107/rcub_cer_7137

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Ristović M, Božić N, Vujčić Z. An innovative process for the production of bioethanol: Optimization and kinetic assessment. in Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia. 2023;:P59.
https://hdl.handle.net/21.15107/rcub_cer_7137 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Ristović, Marina, Božić, Nataša, Vujčić, Zoran, "An innovative process for the production of bioethanol: Optimization and kinetic assessment" in Book of Abstracts - 15th Meeting of the Slovenian Biochemical Society with International Participation, 20-23 September 2023, Portorož, Slovenia (2023):P59,
https://hdl.handle.net/21.15107/rcub_cer_7137 .

TY  - CONF
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Pavlović, Marija
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6027
AB  - When textile dyes are released into the water, they can have a range of negative effects on the environment, for example, azo dyes can break down into carcinogenic compounds when exposed to sunlight. Remazol Brilliant Blue R (RBBR) and Reactive Black 5 (RB5) are reactive dyes. Unfortunately, the discharge of untreated effluents from these industries into nature can result in the contamination of water, which can have harmful effects on the environment.
Saccharomyces cerevisiae is widely used in different fields of biotechnology. In this work, the cell walls of used yeast were reused for the biosorption of textile dyes, and the possibility of their utilization in wastewater treatment was investigated. The response surface methodology approach was applied to determine the optimal levels of three input variables - pH, temperature, and time, and also to identify the relationship between the response functions and process variables. Five levels of three variables were used, pH range 2–13, temperature range 15–40°C and time 0.15–30 h. The decolorization processes were optimized using the Central Composite Design. A significant impact of pH and temperature
was found, while satisfactory results in decolorization (≥ 90%) were shown in a short time (up to 1 h). The maximum adsorption capacity (MAC) of the used Saccharomyces cerevisiae cell wall for removing RBBR and RB5 from wastewater was tested according to results obtained by CCD considering the economy of the process (pH 4, 25°C and 1 hour). Experimentally obtained MAC was 400 mg/g and 200 mg/g for RBBR and RB5, respectively.
PB  - European Federation of Biotechnology
C3  - Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
T1  - Reusing biomass in textile dyes removing from wastewater
SP  - 62
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_cer_6027
ER  - 

@conference{
author = "Margetić, Aleksandra and Šokarda Slavić, Marinela and Ristović, Marina and Stojanović, Sanja and Pavlović, Marija and Vujčić, Zoran",
year = "2023",
abstract = "When textile dyes are released into the water, they can have a range of negative effects on the environment, for example, azo dyes can break down into carcinogenic compounds when exposed to sunlight. Remazol Brilliant Blue R (RBBR) and Reactive Black 5 (RB5) are reactive dyes. Unfortunately, the discharge of untreated effluents from these industries into nature can result in the contamination of water, which can have harmful effects on the environment.
Saccharomyces cerevisiae is widely used in different fields of biotechnology. In this work, the cell walls of used yeast were reused for the biosorption of textile dyes, and the possibility of their utilization in wastewater treatment was investigated. The response surface methodology approach was applied to determine the optimal levels of three input variables - pH, temperature, and time, and also to identify the relationship between the response functions and process variables. Five levels of three variables were used, pH range 2–13, temperature range 15–40°C and time 0.15–30 h. The decolorization processes were optimized using the Central Composite Design. A significant impact of pH and temperature
was found, while satisfactory results in decolorization (≥ 90%) were shown in a short time (up to 1 h). The maximum adsorption capacity (MAC) of the used Saccharomyces cerevisiae cell wall for removing RBBR and RB5 from wastewater was tested according to results obtained by CCD considering the economy of the process (pH 4, 25°C and 1 hour). Experimentally obtained MAC was 400 mg/g and 200 mg/g for RBBR and RB5, respectively.",
publisher = "European Federation of Biotechnology",
journal = "Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online",
title = "Reusing biomass in textile dyes removing from wastewater",
pages = "62-62",
url = "https://hdl.handle.net/21.15107/rcub_cer_6027"
}

Margetić, A., Šokarda Slavić, M., Ristović, M., Stojanović, S., Pavlović, M.,& Vujčić, Z.. (2023). Reusing biomass in textile dyes removing from wastewater. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
European Federation of Biotechnology., 62-62.
https://hdl.handle.net/21.15107/rcub_cer_6027

Margetić A, Šokarda Slavić M, Ristović M, Stojanović S, Pavlović M, Vujčić Z. Reusing biomass in textile dyes removing from wastewater. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online. 2023;:62-62.
https://hdl.handle.net/21.15107/rcub_cer_6027 .

Margetić, Aleksandra, Šokarda Slavić, Marinela, Ristović, Marina, Stojanović, Sanja, Pavlović, Marija, Vujčić, Zoran, "Reusing biomass in textile dyes removing from wastewater" in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online (2023):62-62,
https://hdl.handle.net/21.15107/rcub_cer_6027 .

TY  - CONF
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Ristović, Marina
AU  - Pavlović, Marija
AU  - Stojanović, Sanja
AU  - Drulović, Nenad
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6028
AB  - Fish processing generates a large amount of waste products; nearly 75 % of the total fish weight consists of scales, skins, heads, viscera, and bones. These waste by-products are often dumped in landfills or the ocean, contributing to environmental pollution. Solid fish waste has a high collagen content and can be utilized for the extraction of different products such as gelatine, which can be used in different food and pharmaceutical industries. Fish gelatine contains all biogenic amino acids and can be used as a dietary supplement, thus increasing the utilization of fish waste and reducing environmental pollution.
Recently, a strong worldwide focus has been directed towards the discovery of new microbial proteases for industrial applications. For that purpose, the Bacillus sp. strains from the laboratory bank were screened for the ability to secrete alkaline proteases. Bacterial strains that produced a larger clear zone on the gelatine agar plate were selected for further study. Selected bacterial strains were cultivated under submerged conditions for the purpose of producing proteases. The supernatant from each isolate was used as a crude enzyme extract for testing gelatine proteolytic activity and hydrolysis products. Bacillus sp. strains 12B, 16A, 17B and 24B showed a high degree of hydrolysis of the gelatine waste and the ability to form proteins of small molecular masses after one hour of hydrolysis. Of these, sp. 12B was the most potent, having twice the proteolytic activity determined by the TNBS method.
PB  - European Federation of Biotechnology
C3  - Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
T1  - Screening of Bacillus sp. protease for hydrolysis of gelatine extracted from fish waste and potential applications for waste valorisation
SP  - 66
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_cer_6028
ER  - 

@conference{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Ristović, Marina and Pavlović, Marija and Stojanović, Sanja and Drulović, Nenad and Vujčić, Zoran",
year = "2023",
abstract = "Fish processing generates a large amount of waste products; nearly 75 % of the total fish weight consists of scales, skins, heads, viscera, and bones. These waste by-products are often dumped in landfills or the ocean, contributing to environmental pollution. Solid fish waste has a high collagen content and can be utilized for the extraction of different products such as gelatine, which can be used in different food and pharmaceutical industries. Fish gelatine contains all biogenic amino acids and can be used as a dietary supplement, thus increasing the utilization of fish waste and reducing environmental pollution.
Recently, a strong worldwide focus has been directed towards the discovery of new microbial proteases for industrial applications. For that purpose, the Bacillus sp. strains from the laboratory bank were screened for the ability to secrete alkaline proteases. Bacterial strains that produced a larger clear zone on the gelatine agar plate were selected for further study. Selected bacterial strains were cultivated under submerged conditions for the purpose of producing proteases. The supernatant from each isolate was used as a crude enzyme extract for testing gelatine proteolytic activity and hydrolysis products. Bacillus sp. strains 12B, 16A, 17B and 24B showed a high degree of hydrolysis of the gelatine waste and the ability to form proteins of small molecular masses after one hour of hydrolysis. Of these, sp. 12B was the most potent, having twice the proteolytic activity determined by the TNBS method.",
publisher = "European Federation of Biotechnology",
journal = "Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online",
title = "Screening of Bacillus sp. protease for hydrolysis of gelatine extracted from fish waste and potential applications for waste valorisation",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_cer_6028"
}

Šokarda Slavić, M., Margetić, A., Ristović, M., Pavlović, M., Stojanović, S., Drulović, N.,& Vujčić, Z.. (2023). Screening of Bacillus sp. protease for hydrolysis of gelatine extracted from fish waste and potential applications for waste valorisation. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online
European Federation of Biotechnology., 66-66.
https://hdl.handle.net/21.15107/rcub_cer_6028

Šokarda Slavić M, Margetić A, Ristović M, Pavlović M, Stojanović S, Drulović N, Vujčić Z. Screening of Bacillus sp. protease for hydrolysis of gelatine extracted from fish waste and potential applications for waste valorisation. in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online. 2023;:66-66.
https://hdl.handle.net/21.15107/rcub_cer_6028 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Ristović, Marina, Pavlović, Marija, Stojanović, Sanja, Drulović, Nenad, Vujčić, Zoran, "Screening of Bacillus sp. protease for hydrolysis of gelatine extracted from fish waste and potential applications for waste valorisation" in Programme and abstract book - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 28-29 March 2023, online (2023):66-66,
https://hdl.handle.net/21.15107/rcub_cer_6028 .

TY  - CONF
AU  - Pavlović, Marija
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Momčilović, Miloš
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6241
AB  - Pectinases are a type of enzymes frequently used in the food industry to clarify, liquefy, and stabilize fruit juices. The main challenge in fruit juice production is the cloudiness of the juice, which is largely caused by the presence of pectic polysaccharides. Endo-pectinases are enzymes that hydrolyze the glycosidic bonds in pectic polymers. Commercial pectinolytic enzymes are typically produced by fungi, with Aspergillus spp. being the most commonly used.
The aim of this research was the production and characterization of a novel endo-pectinase from the Aspergillus tubingensis strain for use in liquefying and clarifying different types of fruit juice. To accomplish this, solid-state fermentation was conducted on agricultural waste, such as sugar beet pulp and wheat bran, to produce pectinolytic enzymes. The resulting crude extract was concentrated via ultrafiltration and used to isolate the endo-pectinase via ammonium sulfate and ethanol precipitation methods. Ion-exchange chromatography technique on DEAE Sephadex A-25 matrix was used for further purification of the endo-pectinase.
The purified enzyme was characterized by the determination of total pectinolytic activity, specific pectinolytic activity, and SDS-PAA gel electrophoresis. The activity of the endo-pectinase was confirmed by a diffusion test and zymography with Ruthenium Red visualization. The resulting enzyme was used to liquefy apricot, banana, apple, quince, strawberry, and orange pulp, with juice yields ranging from 71% to 83%, depending on the fruit used. The juices treated with endo-pectinase showed much higher clarification compared to untreated juices. Additionally, the treated juices demonstrated more pronounced antioxidant properties, as determined through the DPPH assay.
PB  - Serbian Chemical Society
C3  - Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Highly active endo-pectinases from Aspergillus tubingensis: A novel enzyme for fruit processing
SP  - 207
EP  - 207
UR  - https://hdl.handle.net/21.15107/rcub_cer_6241
ER  - 

@conference{
author = "Pavlović, Marija and Šokarda Slavić, Marinela and Ristović, Marina and Margetić, Aleksandra and Stojanović, Sanja and Momčilović, Miloš and Vujčić, Zoran",
year = "2023",
abstract = "Pectinases are a type of enzymes frequently used in the food industry to clarify, liquefy, and stabilize fruit juices. The main challenge in fruit juice production is the cloudiness of the juice, which is largely caused by the presence of pectic polysaccharides. Endo-pectinases are enzymes that hydrolyze the glycosidic bonds in pectic polymers. Commercial pectinolytic enzymes are typically produced by fungi, with Aspergillus spp. being the most commonly used.
The aim of this research was the production and characterization of a novel endo-pectinase from the Aspergillus tubingensis strain for use in liquefying and clarifying different types of fruit juice. To accomplish this, solid-state fermentation was conducted on agricultural waste, such as sugar beet pulp and wheat bran, to produce pectinolytic enzymes. The resulting crude extract was concentrated via ultrafiltration and used to isolate the endo-pectinase via ammonium sulfate and ethanol precipitation methods. Ion-exchange chromatography technique on DEAE Sephadex A-25 matrix was used for further purification of the endo-pectinase.
The purified enzyme was characterized by the determination of total pectinolytic activity, specific pectinolytic activity, and SDS-PAA gel electrophoresis. The activity of the endo-pectinase was confirmed by a diffusion test and zymography with Ruthenium Red visualization. The resulting enzyme was used to liquefy apricot, banana, apple, quince, strawberry, and orange pulp, with juice yields ranging from 71% to 83%, depending on the fruit used. The juices treated with endo-pectinase showed much higher clarification compared to untreated juices. Additionally, the treated juices demonstrated more pronounced antioxidant properties, as determined through the DPPH assay.",
publisher = "Serbian Chemical Society",
journal = "Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Highly active endo-pectinases from Aspergillus tubingensis: A novel enzyme for fruit processing",
pages = "207-207",
url = "https://hdl.handle.net/21.15107/rcub_cer_6241"
}

Pavlović, M., Šokarda Slavić, M., Ristović, M., Margetić, A., Stojanović, S., Momčilović, M.,& Vujčić, Z.. (2023). Highly active endo-pectinases from Aspergillus tubingensis: A novel enzyme for fruit processing. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Serbian Chemical Society., 207-207.
https://hdl.handle.net/21.15107/rcub_cer_6241

Pavlović M, Šokarda Slavić M, Ristović M, Margetić A, Stojanović S, Momčilović M, Vujčić Z. Highly active endo-pectinases from Aspergillus tubingensis: A novel enzyme for fruit processing. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:207-207.
https://hdl.handle.net/21.15107/rcub_cer_6241 .

Pavlović, Marija, Šokarda Slavić, Marinela, Ristović, Marina, Margetić, Aleksandra, Stojanović, Sanja, Momčilović, Miloš, Vujčić, Zoran, "Highly active endo-pectinases from Aspergillus tubingensis: A novel enzyme for fruit processing" in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):207-207,
https://hdl.handle.net/21.15107/rcub_cer_6241 .

TY  - CONF
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Pavlović, Marija
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6243
AB  - Xylan makes a significant part of cereals and fruits, which are used in the food industry. Therefore, enzymes that hydrolyze xylan (xylanases) have found application in the modification of cereal-based food, improving the digestibility of animal feed, and improving the texture of bakery products. In the juice industry, the main problems are turbidity, viscosity, and sedimentation during standing, which are caused by polysaccharides present in fruit (pectins, cellulose, and hemicellulose (xylan)). Pineapple, apple, orange, and tomato have a high content of hemicellulose, so xylanases are suitable for improving the properties of these juices. The Aspergillus tubingensis FAT 35 strain (considered safe for use in the food industry) growing on SSF medium composed of corn cob produced a high level of xylanase enzyme (4.03 U∕mL) and not that high pectinase (1.02 U∕mL) and cellulase (1.43 U∕mL) activities at pH 3 which is pH of freshly prepared apple, pineapple and organge juice.. The fermentation extract was used for clarification of pineapple, apple, and orange juice and for increasing the filtration rate and yield of these juices. Results indicate that A. tubigensis xylanase could be used for clarification and improvement of properties of juices of fruits that contain hemicellulose in high proportion.
PB  - Serbian Chemical Society
C3  - Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
T1  - Highly active xylanase used in juice clarification
SP  - 208
EP  - 208
UR  - https://hdl.handle.net/21.15107/rcub_cer_6243
ER  - 

@conference{
author = "Ristović, Marina and Stojanović, Sanja and Pavlović, Marija and Margetić, Aleksandra and Šokarda Slavić, Marinela and Vujčić, Zoran and Dojnov, Biljana",
year = "2023",
abstract = "Xylan makes a significant part of cereals and fruits, which are used in the food industry. Therefore, enzymes that hydrolyze xylan (xylanases) have found application in the modification of cereal-based food, improving the digestibility of animal feed, and improving the texture of bakery products. In the juice industry, the main problems are turbidity, viscosity, and sedimentation during standing, which are caused by polysaccharides present in fruit (pectins, cellulose, and hemicellulose (xylan)). Pineapple, apple, orange, and tomato have a high content of hemicellulose, so xylanases are suitable for improving the properties of these juices. The Aspergillus tubingensis FAT 35 strain (considered safe for use in the food industry) growing on SSF medium composed of corn cob produced a high level of xylanase enzyme (4.03 U∕mL) and not that high pectinase (1.02 U∕mL) and cellulase (1.43 U∕mL) activities at pH 3 which is pH of freshly prepared apple, pineapple and organge juice.. The fermentation extract was used for clarification of pineapple, apple, and orange juice and for increasing the filtration rate and yield of these juices. Results indicate that A. tubigensis xylanase could be used for clarification and improvement of properties of juices of fruits that contain hemicellulose in high proportion.",
publisher = "Serbian Chemical Society",
journal = "Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia",
title = "Highly active xylanase used in juice clarification",
pages = "208-208",
url = "https://hdl.handle.net/21.15107/rcub_cer_6243"
}

Ristović, M., Stojanović, S., Pavlović, M., Margetić, A., Šokarda Slavić, M., Vujčić, Z.,& Dojnov, B.. (2023). Highly active xylanase used in juice clarification. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia
Serbian Chemical Society., 208-208.
https://hdl.handle.net/21.15107/rcub_cer_6243

Ristović M, Stojanović S, Pavlović M, Margetić A, Šokarda Slavić M, Vujčić Z, Dojnov B. Highly active xylanase used in juice clarification. in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia. 2023;:208-208.
https://hdl.handle.net/21.15107/rcub_cer_6243 .

Ristović, Marina, Stojanović, Sanja, Pavlović, Marija, Margetić, Aleksandra, Šokarda Slavić, Marinela, Vujčić, Zoran, Dojnov, Biljana, "Highly active xylanase used in juice clarification" in Abstract Book - XXII Congress EuroFoodChem, June 14-16, 2023, Belgrade, Serbia (2023):208-208,
https://hdl.handle.net/21.15107/rcub_cer_6243 .

TY  - JOUR
AU  - Pavlović, Marija
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Momčilović, Miloš
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6459
AB  - The main goal of this study was to examine the efficiency of a newly isolated fungus from quince, Aspergillus tubingensis FAT43, to produce the pectinolytic complex using agricultural and industrial waste as the substrate for solid state fermentation. Sugar beet pulp was the most effective substrate inducer of pectinolytic complex synthesis out of all the waste residues examined. For endo-pectinolytic and total pectinolytic activity, respectively, statistical optimization using Placked-Burman Design and Optimal (Custom) Design increased production by 2.22 and 2.15-fold, respectively. Liquification, clarification, and an increase in the amount of reducing sugar in fruit juices (apple, banana, apricot, orange, and quince) processed with pectinolytic complex were identified. Enzymatic pre-treatment considerably increases yield (14%–22%) and clarification (90%). After enzymatic treatment, the best liquefaction was observed in orange juice, whereas the best clarification was obtained in apricot juice. Additionally, the pectinolytic treatment of apricot juice resulted in the highest increase in reducing sugar concentration (11%) compared to all other enzymatically treated juices. Optimizing the production of a highly active pectinolytic complex and its efficient utilization in the processing of fruit juices, including the generation of an increasing amount of waste, are the significant outcomes of this research.
PB  - Oxford University Press
T2  - Letters in Applied Microbiology
T1  - Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing
VL  - 76
IS  - 8
SP  - 1
EP  - 10
DO  - 10.1093/lambio/ovad083
ER  - 

@article{
author = "Pavlović, Marija and Šokarda Slavić, Marinela and Ristović, Marina and Stojanović, Sanja and Margetić, Aleksandra and Momčilović, Miloš and Vujčić, Zoran",
year = "2023",
abstract = "The main goal of this study was to examine the efficiency of a newly isolated fungus from quince, Aspergillus tubingensis FAT43, to produce the pectinolytic complex using agricultural and industrial waste as the substrate for solid state fermentation. Sugar beet pulp was the most effective substrate inducer of pectinolytic complex synthesis out of all the waste residues examined. For endo-pectinolytic and total pectinolytic activity, respectively, statistical optimization using Placked-Burman Design and Optimal (Custom) Design increased production by 2.22 and 2.15-fold, respectively. Liquification, clarification, and an increase in the amount of reducing sugar in fruit juices (apple, banana, apricot, orange, and quince) processed with pectinolytic complex were identified. Enzymatic pre-treatment considerably increases yield (14%–22%) and clarification (90%). After enzymatic treatment, the best liquefaction was observed in orange juice, whereas the best clarification was obtained in apricot juice. Additionally, the pectinolytic treatment of apricot juice resulted in the highest increase in reducing sugar concentration (11%) compared to all other enzymatically treated juices. Optimizing the production of a highly active pectinolytic complex and its efficient utilization in the processing of fruit juices, including the generation of an increasing amount of waste, are the significant outcomes of this research.",
publisher = "Oxford University Press",
journal = "Letters in Applied Microbiology",
title = "Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing",
volume = "76",
number = "8",
pages = "1-10",
doi = "10.1093/lambio/ovad083"
}

Pavlović, M., Šokarda Slavić, M., Ristović, M., Stojanović, S., Margetić, A., Momčilović, M.,& Vujčić, Z.. (2023). Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing. in Letters in Applied Microbiology
Oxford University Press., 76(8), 1-10.
https://doi.org/10.1093/lambio/ovad083

Pavlović M, Šokarda Slavić M, Ristović M, Stojanović S, Margetić A, Momčilović M, Vujčić Z. Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing. in Letters in Applied Microbiology. 2023;76(8):1-10.
doi:10.1093/lambio/ovad083 .

Pavlović, Marija, Šokarda Slavić, Marinela, Ristović, Marina, Stojanović, Sanja, Margetić, Aleksandra, Momčilović, Miloš, Vujčić, Zoran, "Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing" in Letters in Applied Microbiology, 76, no. 8 (2023):1-10,
https://doi.org/10.1093/lambio/ovad083 . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Ristović, Marina
AU  - Pavlović, Marija
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6518
AB  - The combination of b-oligosaccharides from enzymatically hydrolysed barley b-glucan has attracted interest
recently due to its positive effects on human health. This study aimed to assess the impact of the
endo-b-1,3-1,4-glucanase enzyme from Bacillus subtilis 168 on improving the nutritional and bioactive
properties of barley b-glucan. A new procedure for the isolation of b-glucan was developed, at a lower
temperature (45 °C), enabling purity from starch contamination, without affecting the yield (6 g b-glucan
from 100 g of barley flour). The endo-b-1,3-1,4-glucanase is cloned into E. coli pQE_Ek enables the high
production and purification (82% yield, 1.8 mg mL
 1 and 440 U mg
 1) of an enzyme identical to the
natural one (25.5 kDa). The enzymatic reaction showed high efficiency of b-glucan degradation by recombinant
enzyme, giving a mixture of products (of which 3-O-b-cellobiosyl-D-glucose and 3-O-b-cellotriosyl-
D-glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacities
by 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicate
the possible application of endo-b-1,3-1,4-glucanase enzyme in improving the properties of barley bglucan
used as functional foods.
PB  - Wiley
T2  - International Journal of Food Science and Technology
T1  - Improvement of nutritional and bioactive properties of barley β-glucan-based food products using Bacillus subtilis 168 endo-β-1,3-1,4-glucanase
DO  - 10.1111/ijfs.16647
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Kojić, Milan and Margetić, Aleksandra and Ristović, Marina and Pavlović, Marija and Nikolić, Stefan and Vujčić, Zoran",
year = "2023",
abstract = "The combination of b-oligosaccharides from enzymatically hydrolysed barley b-glucan has attracted interest
recently due to its positive effects on human health. This study aimed to assess the impact of the
endo-b-1,3-1,4-glucanase enzyme from Bacillus subtilis 168 on improving the nutritional and bioactive
properties of barley b-glucan. A new procedure for the isolation of b-glucan was developed, at a lower
temperature (45 °C), enabling purity from starch contamination, without affecting the yield (6 g b-glucan
from 100 g of barley flour). The endo-b-1,3-1,4-glucanase is cloned into E. coli pQE_Ek enables the high
production and purification (82% yield, 1.8 mg mL
 1 and 440 U mg
 1) of an enzyme identical to the
natural one (25.5 kDa). The enzymatic reaction showed high efficiency of b-glucan degradation by recombinant
enzyme, giving a mixture of products (of which 3-O-b-cellobiosyl-D-glucose and 3-O-b-cellotriosyl-
D-glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacities
by 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicate
the possible application of endo-b-1,3-1,4-glucanase enzyme in improving the properties of barley bglucan
used as functional foods.",
publisher = "Wiley",
journal = "International Journal of Food Science and Technology",
title = "Improvement of nutritional and bioactive properties of barley β-glucan-based food products using Bacillus subtilis 168 endo-β-1,3-1,4-glucanase",
doi = "10.1111/ijfs.16647"
}

Šokarda Slavić, M., Kojić, M., Margetić, A., Ristović, M., Pavlović, M., Nikolić, S.,& Vujčić, Z.. (2023). Improvement of nutritional and bioactive properties of barley β-glucan-based food products using Bacillus subtilis 168 endo-β-1,3-1,4-glucanase. in International Journal of Food Science and Technology
Wiley..
https://doi.org/10.1111/ijfs.16647

Šokarda Slavić M, Kojić M, Margetić A, Ristović M, Pavlović M, Nikolić S, Vujčić Z. Improvement of nutritional and bioactive properties of barley β-glucan-based food products using Bacillus subtilis 168 endo-β-1,3-1,4-glucanase. in International Journal of Food Science and Technology. 2023;.
doi:10.1111/ijfs.16647 .

Šokarda Slavić, Marinela, Kojić, Milan, Margetić, Aleksandra, Ristović, Marina, Pavlović, Marija, Nikolić, Stefan, Vujčić, Zoran, "Improvement of nutritional and bioactive properties of barley β-glucan-based food products using Bacillus subtilis 168 endo-β-1,3-1,4-glucanase" in International Journal of Food Science and Technology (2023),
https://doi.org/10.1111/ijfs.16647 . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Ralić, Vanja
AU  - Nastasijević, Branislav
AU  - Matijević, Milica
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6460
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7407
AB  - Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.
AB  - Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
T1  - Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B
VL  - 88
IS  - 10
SP  - 985
EP  - 997
DO  - 10.2298/JSC221116011S
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Ralić, Vanja and Nastasijević, Branislav and Matijević, Milica and Vujčić, Zoran and Margetić, Aleksandra",
year = "2023",
abstract = "Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone., Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain, Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B",
volume = "88",
number = "10",
pages = "985-997",
doi = "10.2298/JSC221116011S"
}

Šokarda Slavić, M., Ralić, V., Nastasijević, B., Matijević, M., Vujčić, Z.,& Margetić, A.. (2023). A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(10), 985-997.
https://doi.org/10.2298/JSC221116011S

Šokarda Slavić M, Ralić V, Nastasijević B, Matijević M, Vujčić Z, Margetić A. A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society. 2023;88(10):985-997.
doi:10.2298/JSC221116011S .

Šokarda Slavić, Marinela, Ralić, Vanja, Nastasijević, Branislav, Matijević, Milica, Vujčić, Zoran, Margetić, Aleksandra, "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain" in Journal of the Serbian Chemical Society, 88, no. 10 (2023):985-997,
https://doi.org/10.2298/JSC221116011S . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Ralić, Vanja
AU  - Nastasijević, Branislav
AU  - Matijević, Milica
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6460
AB  - Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.
AB  - Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на 
ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална 
употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2
показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
T1  - Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B
VL  - 88
IS  - 10
SP  - 985
EP  - 997
DO  - 10.2298/JSC221116011S
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Ralić, Vanja and Nastasijević, Branislav and Matijević, Milica and Vujčić, Zoran and Margetić, Aleksandra",
year = "2023",
abstract = "Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone., Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на 
ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална 
употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2
показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain, Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B",
volume = "88",
number = "10",
pages = "985-997",
doi = "10.2298/JSC221116011S"
}

Šokarda Slavić, M., Ralić, V., Nastasijević, B., Matijević, M., Vujčić, Z.,& Margetić, A.. (2023). A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(10), 985-997.
https://doi.org/10.2298/JSC221116011S

Šokarda Slavić M, Ralić V, Nastasijević B, Matijević M, Vujčić Z, Margetić A. A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society. 2023;88(10):985-997.
doi:10.2298/JSC221116011S .

Šokarda Slavić, Marinela, Ralić, Vanja, Nastasijević, Branislav, Matijević, Milica, Vujčić, Zoran, Margetić, Aleksandra, "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain" in Journal of the Serbian Chemical Society, 88, no. 10 (2023):985-997,
https://doi.org/10.2298/JSC221116011S . .

TY  - CONF
AU  - Pavlović, Marija
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Pavlović, Ratko
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5840
AB  - Pectinases are widely used in the fruit juice industry for clarification, liquefaction and stabilization of juices. One of the biggest problems in the production of fruit juices is the turbidity of the juice, which is mainly caused by the presence of pectin polysaccharides. Therefore, pectinase is used in juice clarification, which breaks down the pectin structure and reduces unwanted cloudiness and sediment. In this work, the production of pectinases was optimized by solid state fermentation using Aspergillus tubingensis strain, which proved to be an efficient producer of these enzymes. Statistical method Design of Experiment was used to optimize the medium and conditions for enzyme production. The total pectinase activity obtained was determined by the DNS method (47 U/mL). Endo-pectinases activity is determined by reduction of viscosity of pectin solutions. The resulting complex of pectinase enzymes was used for the liquefaction of apricot and blueberry pulp, with a juice yield of 72% and 81%, respectively. Also, apricot juice treated with enzymes was clarified by 77% compared to juice that was not treated with enzymes. Blueberry juice obtained after treatment with pectinase enzymes has a higher antioxidant activity than the untreated juice, as determined by the DPPH assay.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
T1  - Production and application of pectinases in the liquefaction of apricot and blueberry juice
SP  - 115
EP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_cer_5840
ER  - 

@conference{
author = "Pavlović, Marija and Margetić, Aleksandra and Šokarda Slavić, Marinela and Ristović, Marina and Pavlović, Ratko and Nikolić, Stefan and Vujčić, Zoran",
year = "2022",
abstract = "Pectinases are widely used in the fruit juice industry for clarification, liquefaction and stabilization of juices. One of the biggest problems in the production of fruit juices is the turbidity of the juice, which is mainly caused by the presence of pectin polysaccharides. Therefore, pectinase is used in juice clarification, which breaks down the pectin structure and reduces unwanted cloudiness and sediment. In this work, the production of pectinases was optimized by solid state fermentation using Aspergillus tubingensis strain, which proved to be an efficient producer of these enzymes. Statistical method Design of Experiment was used to optimize the medium and conditions for enzyme production. The total pectinase activity obtained was determined by the DNS method (47 U/mL). Endo-pectinases activity is determined by reduction of viscosity of pectin solutions. The resulting complex of pectinase enzymes was used for the liquefaction of apricot and blueberry pulp, with a juice yield of 72% and 81%, respectively. Also, apricot juice treated with enzymes was clarified by 77% compared to juice that was not treated with enzymes. Blueberry juice obtained after treatment with pectinase enzymes has a higher antioxidant activity than the untreated juice, as determined by the DPPH assay.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia",
title = "Production and application of pectinases in the liquefaction of apricot and blueberry juice",
pages = "115-116",
url = "https://hdl.handle.net/21.15107/rcub_cer_5840"
}

Pavlović, M., Margetić, A., Šokarda Slavić, M., Ristović, M., Pavlović, R., Nikolić, S.,& Vujčić, Z.. (2022). Production and application of pectinases in the liquefaction of apricot and blueberry juice. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
University of Belgrade - Faculty of Chemistry., 115-116.
https://hdl.handle.net/21.15107/rcub_cer_5840

Pavlović M, Margetić A, Šokarda Slavić M, Ristović M, Pavlović R, Nikolić S, Vujčić Z. Production and application of pectinases in the liquefaction of apricot and blueberry juice. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia. 2022;:115-116.
https://hdl.handle.net/21.15107/rcub_cer_5840 .

Pavlović, Marija, Margetić, Aleksandra, Šokarda Slavić, Marinela, Ristović, Marina, Pavlović, Ratko, Nikolić, Stefan, Vujčić, Zoran, "Production and application of pectinases in the liquefaction of apricot and blueberry juice" in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia (2022):115-116,
https://hdl.handle.net/21.15107/rcub_cer_5840 .

TY  - JOUR
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Stepanović, Jelena
AU  - Margetić, Aleksandra
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5360
AB  - Production of fructooligosaccharides (FOS) is a trending topic due to their prebiotic effect becoming increasingly important for the modern human diet. The most suitable process for FOS production is the one using fungal inulinases. Introduction of new fungal inulinase producers and their implementation in production of inulinase enzymes is therefore gaining interest. This study provides a new approach to FOS synthesis by fungal enzyme complex without prior separation of any specific enzyme. Inulinase enzyme complexes could be used for the synthesis of FOS in two possible ways – hydrolysis of inulin (FOSh) and transfructosylation process of sucrose (FOSs), as demonstrated here. Depending on the fungal growth inducing substrate, a variety of inulinase enzyme complexes was obtained – one of which was most successful in production of FOSh and another one of FOSs. Substrates derived from crops: triticale, wheat bran, Jerusalem artichoke and Aspergillus welwitschiae isolate, previously proven as safe for use in food, were utilized for production of inulinase enzyme cocktails. The highest FOSs production was obtained by enzyme complex rich in β-fructofuranosidase, while the highest FOSh production was obtained by enzyme complex rich in endoinulinase. Both FOSh and FOSs showed antioxidant potential according to ABTS and ORAC, which classifies them as a suitable additive in functional food. Simultaneous zymographic detection of inulinase enzymes, which could contribute to expansion of the knowledge on fungal enzymes, was developed and applied here. It demonstrated the presence of different inulinase isoforms depending on fungal growth substrate. These findings, which rely on the innate ability of fungi to co-produce all inulinases from a cocktail, could be useful as a new, easy approach to FOS production by fungal enzymes without their separation and purification, contributing to cheaper and faster production processes.
PB  - Elsevier
T2  - Food Research International
T1  - Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production
VL  - 160
SP  - 111755
DO  - 10.1016/j.foodres.2022.111755
ER  - 

@article{
author = "Stojanović, Sanja and Ristović, Marina and Stepanović, Jelena and Margetić, Aleksandra and Duduk, Bojan and Vujčić, Zoran and Dojnov, Biljana",
year = "2022",
abstract = "Production of fructooligosaccharides (FOS) is a trending topic due to their prebiotic effect becoming increasingly important for the modern human diet. The most suitable process for FOS production is the one using fungal inulinases. Introduction of new fungal inulinase producers and their implementation in production of inulinase enzymes is therefore gaining interest. This study provides a new approach to FOS synthesis by fungal enzyme complex without prior separation of any specific enzyme. Inulinase enzyme complexes could be used for the synthesis of FOS in two possible ways – hydrolysis of inulin (FOSh) and transfructosylation process of sucrose (FOSs), as demonstrated here. Depending on the fungal growth inducing substrate, a variety of inulinase enzyme complexes was obtained – one of which was most successful in production of FOSh and another one of FOSs. Substrates derived from crops: triticale, wheat bran, Jerusalem artichoke and Aspergillus welwitschiae isolate, previously proven as safe for use in food, were utilized for production of inulinase enzyme cocktails. The highest FOSs production was obtained by enzyme complex rich in β-fructofuranosidase, while the highest FOSh production was obtained by enzyme complex rich in endoinulinase. Both FOSh and FOSs showed antioxidant potential according to ABTS and ORAC, which classifies them as a suitable additive in functional food. Simultaneous zymographic detection of inulinase enzymes, which could contribute to expansion of the knowledge on fungal enzymes, was developed and applied here. It demonstrated the presence of different inulinase isoforms depending on fungal growth substrate. These findings, which rely on the innate ability of fungi to co-produce all inulinases from a cocktail, could be useful as a new, easy approach to FOS production by fungal enzymes without their separation and purification, contributing to cheaper and faster production processes.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production",
volume = "160",
pages = "111755",
doi = "10.1016/j.foodres.2022.111755"
}

Stojanović, S., Ristović, M., Stepanović, J., Margetić, A., Duduk, B., Vujčić, Z.,& Dojnov, B.. (2022). Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production. in Food Research International
Elsevier., 160, 111755.
https://doi.org/10.1016/j.foodres.2022.111755

Stojanović S, Ristović M, Stepanović J, Margetić A, Duduk B, Vujčić Z, Dojnov B. Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production. in Food Research International. 2022;160:111755.
doi:10.1016/j.foodres.2022.111755 .

Stojanović, Sanja, Ristović, Marina, Stepanović, Jelena, Margetić, Aleksandra, Duduk, Bojan, Vujčić, Zoran, Dojnov, Biljana, "Aspergillus welwitschiae inulinase enzyme cocktails obtained on agro-material inducers for the purpose of fructooligosaccharides production" in Food Research International, 160 (2022):111755,
https://doi.org/10.1016/j.foodres.2022.111755 . .

TY  - JOUR
AU  - Margetić, Aleksandra
AU  - Nikolić, Stefan
AU  - Grgurić-Šipka, Sanja
AU  - Vujčić, Miroslava
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5512
AB  - The interaction of four arene ruthenium complexes [(η6-p-cymene)Ru(Me2dppz)Cl]PF6 (1) with Me2dppz = 11,12-dimethyldipyrido[3,2-a:2′,3′-c]phenazine, [(η6-p-cymene)Ru(aip)Cl]PF6 (2) with aip = 2-(9-anthryl)-1H-imidazo[4,5-f][1,10] phenanthroline), ([(ƞ6-toluene)Ru(ppf)Cl]PF6) (3) and ([(ƞ6-p-cymene)Ru(ppf)Cl]PF6) (4) with ppf = pyrido[2′,3′:5,6] pyrazino[2,3-f][1,10]phenanthroline with calf thymus DNA were investigated. All of four complexes exhibit DNA-binding activity. UV–Vis spectroscopic studies revealed the intrinsic binding constants of the order 104 M−1 of magnitude, indicating non-intercalative mode. Fluorescence quenching analysis showed that all complexes interfere with intercalator ethidium bromide and minor groove binder Hoechst 33258 by a singular non-intercalative mode with extent that differs by two orders of magnitude. Gel electrophoresis results on DNA cleavage assay demonstrated that all complexes produced conformational changes of supercoiled circular plasmid pUC19 in concentration dependent way. The results of fluorescence titration bovine serum albumin by 1, 2, 3 and 4 showed that all complexes significantly quench tryptophan residues fluorescence through a static quenching mechanism. The antimicrobial activity against both Gram-positive and Gram-negative bacteria analyzed. Complex 1 was most active, even on Escherichia coli was more active than positive control compound.
PB  - Springer
T2  - BioMetals
T1  - Interaction of organoruthenium(II)-polypyridyl complexes with DNA and BSA
VL  - 35
IS  - 4
SP  - 813
EP  - 829
DO  - 10.1007/s10534-022-00404-6
ER  - 

@article{
author = "Margetić, Aleksandra and Nikolić, Stefan and Grgurić-Šipka, Sanja and Vujčić, Miroslava",
year = "2022",
abstract = "The interaction of four arene ruthenium complexes [(η6-p-cymene)Ru(Me2dppz)Cl]PF6 (1) with Me2dppz = 11,12-dimethyldipyrido[3,2-a:2′,3′-c]phenazine, [(η6-p-cymene)Ru(aip)Cl]PF6 (2) with aip = 2-(9-anthryl)-1H-imidazo[4,5-f][1,10] phenanthroline), ([(ƞ6-toluene)Ru(ppf)Cl]PF6) (3) and ([(ƞ6-p-cymene)Ru(ppf)Cl]PF6) (4) with ppf = pyrido[2′,3′:5,6] pyrazino[2,3-f][1,10]phenanthroline with calf thymus DNA were investigated. All of four complexes exhibit DNA-binding activity. UV–Vis spectroscopic studies revealed the intrinsic binding constants of the order 104 M−1 of magnitude, indicating non-intercalative mode. Fluorescence quenching analysis showed that all complexes interfere with intercalator ethidium bromide and minor groove binder Hoechst 33258 by a singular non-intercalative mode with extent that differs by two orders of magnitude. Gel electrophoresis results on DNA cleavage assay demonstrated that all complexes produced conformational changes of supercoiled circular plasmid pUC19 in concentration dependent way. The results of fluorescence titration bovine serum albumin by 1, 2, 3 and 4 showed that all complexes significantly quench tryptophan residues fluorescence through a static quenching mechanism. The antimicrobial activity against both Gram-positive and Gram-negative bacteria analyzed. Complex 1 was most active, even on Escherichia coli was more active than positive control compound.",
publisher = "Springer",
journal = "BioMetals",
title = "Interaction of organoruthenium(II)-polypyridyl complexes with DNA and BSA",
volume = "35",
number = "4",
pages = "813-829",
doi = "10.1007/s10534-022-00404-6"
}

Margetić, A., Nikolić, S., Grgurić-Šipka, S.,& Vujčić, M.. (2022). Interaction of organoruthenium(II)-polypyridyl complexes with DNA and BSA. in BioMetals
Springer., 35(4), 813-829.
https://doi.org/10.1007/s10534-022-00404-6

Margetić A, Nikolić S, Grgurić-Šipka S, Vujčić M. Interaction of organoruthenium(II)-polypyridyl complexes with DNA and BSA. in BioMetals. 2022;35(4):813-829.
doi:10.1007/s10534-022-00404-6 .

Margetić, Aleksandra, Nikolić, Stefan, Grgurić-Šipka, Sanja, Vujčić, Miroslava, "Interaction of organoruthenium(II)-polypyridyl complexes with DNA and BSA" in BioMetals, 35, no. 4 (2022):813-829,
https://doi.org/10.1007/s10534-022-00404-6 . .

TY  - CONF
AU  - Mišić, Milan
AU  - Drulović, Nenad
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5841
AB  - Polyphenols are a large group of natural organic compounds mainly found in plants, in whom they have diverse protective and metabolic functions. It’s also known that phenolic compounds, especially tannins, interact with proteins in various significant and distinct ways. Tannins also complex proteins, which generally precipitates them and it’s their most important industrially utilised characteristic. This study, which was a continuation of our previous work, focused on interactions of aqueous tea plant extracts with laccase from Trametes 63ersicolour and β-amylase from Ipomoea batatas. Tea plants used in this study wereSaturejamontana, Menthapiperita, Salvia officinalis, Matricariachamomilla, Camellia cinensis and Arctostaphylosuva-ursi. Total phenolic content was determined using Folin-Ciocalteu reagent, which showed us that chosen plants vary considerably in their total phenolic content and the highest concentration was found to be in Arctostaphylosuva-ursi. Protein interactions between tea plant extracts were measured using spectrophotometric, spectrofluorimetric and electrophoretic methods. These methods showed that tea plant extracts lead to various structural changes within the protein, nature of which require 
further research. Finally, it was also found that all tea plant extracts, except Matricariachamomillaextract, reversibly precipitate β-amylase – whilst retaining most of 
its enzymatic activity after dissolving.Best results were obtained using Arctostaphylosuva-ursi extract, which precipitated the highest quantity of β-amylase, with highest activity retention. Although being an interesting phenomenon, furtherresearch is necessary to determine the nature andimportance of reversible tannic enzyme precipitation.
PB  - Serbian Chemical Society
C3  - Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
T1  - Protein interactions of six tea plant extracts
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_cer_5841
ER  - 

@conference{
author = "Mišić, Milan and Drulović, Nenad and Margetić, Aleksandra and Vujčić, Zoran and Vujčić, Miroslava",
year = "2022",
abstract = "Polyphenols are a large group of natural organic compounds mainly found in plants, in whom they have diverse protective and metabolic functions. It’s also known that phenolic compounds, especially tannins, interact with proteins in various significant and distinct ways. Tannins also complex proteins, which generally precipitates them and it’s their most important industrially utilised characteristic. This study, which was a continuation of our previous work, focused on interactions of aqueous tea plant extracts with laccase from Trametes 63ersicolour and β-amylase from Ipomoea batatas. Tea plants used in this study wereSaturejamontana, Menthapiperita, Salvia officinalis, Matricariachamomilla, Camellia cinensis and Arctostaphylosuva-ursi. Total phenolic content was determined using Folin-Ciocalteu reagent, which showed us that chosen plants vary considerably in their total phenolic content and the highest concentration was found to be in Arctostaphylosuva-ursi. Protein interactions between tea plant extracts were measured using spectrophotometric, spectrofluorimetric and electrophoretic methods. These methods showed that tea plant extracts lead to various structural changes within the protein, nature of which require 
further research. Finally, it was also found that all tea plant extracts, except Matricariachamomillaextract, reversibly precipitate β-amylase – whilst retaining most of 
its enzymatic activity after dissolving.Best results were obtained using Arctostaphylosuva-ursi extract, which precipitated the highest quantity of β-amylase, with highest activity retention. Although being an interesting phenomenon, furtherresearch is necessary to determine the nature andimportance of reversible tannic enzyme precipitation.",
publisher = "Serbian Chemical Society",
journal = "Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine",
title = "Protein interactions of six tea plant extracts",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_cer_5841"
}

Mišić, M., Drulović, N., Margetić, A., Vujčić, Z.,& Vujčić, M.. (2022). Protein interactions of six tea plant extracts. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
Serbian Chemical Society., 63.
https://hdl.handle.net/21.15107/rcub_cer_5841

Mišić M, Drulović N, Margetić A, Vujčić Z, Vujčić M. Protein interactions of six tea plant extracts. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine. 2022;:63.
https://hdl.handle.net/21.15107/rcub_cer_5841 .

Mišić, Milan, Drulović, Nenad, Margetić, Aleksandra, Vujčić, Zoran, Vujčić, Miroslava, "Protein interactions of six tea plant extracts" in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine (2022):63,
https://hdl.handle.net/21.15107/rcub_cer_5841 .

TY  - CONF
AU  - Pavlović, Marija
AU  - Stojanović, Sanja
AU  - Dojnov, Biljana
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5834
AB  - Pectinolytic enzymes represent a large group of enzymes that catalyze the reactions of depolymerization and deesterification of pectin polysaccharides1. Saprophytic fungi produce pectinases on a large scale for industrial purposes. These enzymes have a various biotechnological application and their global annual production represents 25% of total industrial enzymes 1,2. Agro-waste is widely used as economical substrate for the production of pectinases by solid state fermentation 3. In this study, sugar beet pulp, as a good source of pectin3, was used as a substrate for enzyme production by Aspergillus tubingensis. This strain was isolated from the quince fruit and identified by the molecular DNA marker calmodulin (CaM). SSF was performed with this strain on sugar beet pulp (80%) in combination with wheat bran (20%), a potent substrate for pectinase production 3. The obtained high pectinolytic activity (15 U/mL), determined by 3,5-dinitrosalicylic acid reagent, was in the range of commercial pectinases. Zymography detection, using Ruthenium Red to visualize endo-pectinase activity and pectin-methyl esterase activity revealed several pectinase activity bands. Hydrolysis of different pectin substrates with the obtained pectinase complex was analyzed by thin layer chromatography in order to detect different products such as pectic oligosaccharides, which are emerging prebiotics superior to intact pectin.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Highly active pectinases from newly isolated Aspergillus tubingensis strain
SP  - 124
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_cer_5834
ER  - 

@conference{
author = "Pavlović, Marija and Stojanović, Sanja and Dojnov, Biljana and Božić, Nataša and Vujčić, Zoran and Margetić, Aleksandra",
year = "2021",
abstract = "Pectinolytic enzymes represent a large group of enzymes that catalyze the reactions of depolymerization and deesterification of pectin polysaccharides1. Saprophytic fungi produce pectinases on a large scale for industrial purposes. These enzymes have a various biotechnological application and their global annual production represents 25% of total industrial enzymes 1,2. Agro-waste is widely used as economical substrate for the production of pectinases by solid state fermentation 3. In this study, sugar beet pulp, as a good source of pectin3, was used as a substrate for enzyme production by Aspergillus tubingensis. This strain was isolated from the quince fruit and identified by the molecular DNA marker calmodulin (CaM). SSF was performed with this strain on sugar beet pulp (80%) in combination with wheat bran (20%), a potent substrate for pectinase production 3. The obtained high pectinolytic activity (15 U/mL), determined by 3,5-dinitrosalicylic acid reagent, was in the range of commercial pectinases. Zymography detection, using Ruthenium Red to visualize endo-pectinase activity and pectin-methyl esterase activity revealed several pectinase activity bands. Hydrolysis of different pectin substrates with the obtained pectinase complex was analyzed by thin layer chromatography in order to detect different products such as pectic oligosaccharides, which are emerging prebiotics superior to intact pectin.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Highly active pectinases from newly isolated Aspergillus tubingensis strain",
pages = "124-125",
url = "https://hdl.handle.net/21.15107/rcub_cer_5834"
}

Pavlović, M., Stojanović, S., Dojnov, B., Božić, N., Vujčić, Z.,& Margetić, A.. (2021). Highly active pectinases from newly isolated Aspergillus tubingensis strain. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 124-125.
https://hdl.handle.net/21.15107/rcub_cer_5834

Pavlović M, Stojanović S, Dojnov B, Božić N, Vujčić Z, Margetić A. Highly active pectinases from newly isolated Aspergillus tubingensis strain. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:124-125.
https://hdl.handle.net/21.15107/rcub_cer_5834 .

Pavlović, Marija, Stojanović, Sanja, Dojnov, Biljana, Božić, Nataša, Vujčić, Zoran, Margetić, Aleksandra, "Highly active pectinases from newly isolated Aspergillus tubingensis strain" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):124-125,
https://hdl.handle.net/21.15107/rcub_cer_5834 .

TY  - JOUR
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4540
AB  - There is an urgent need to increase the daily intake of insoluble dietary fiber, and at the same time to find new sources and new production technologies. We hypothesized that fungal enzymes directly involved in lignocellulosic material hydrolysis (Aspergillus and Trichoderma enzyme cocktails) will change the fiber structure particularly efficiently after the action of laccase (Trametes versicolor enzyme cocktail). Enzymes production on an inducing substrate (same as starting material for obtainment of insoluble dietary fibers) and their usage resulted in obtainment of novel insoluble dietary fibers with better characteristics, 24% higher swelling, 43% higher WRC and 57% higher ORC compared to insoluble dietary fibers from triticale (already proven to be a good food additive). Changes in structure were analyzed by FTIR and microscopic analysis. Antioxidative performance of the obtained products, new insoluble and released soluble dietary fibers, was analyzed in detail. Newly obtained soluble dietary fibers demonstrated up to 20 times higher antioxidant activity compared to untreated fibers (ABTS and DPPH tests). These results suggest their good performance as a future food additive. At the same time, they prove the hypothesis that the use of enzyme cocktails rich in laccase is a good choice for biological pretreatment in this process.
PB  - Elsevier
T2  - LWT - Food Science and Technology
T1  - Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale
VL  - 145
SP  - 111291
DO  - 10.1016/j.lwt.2021.111291
ER  - 

@article{
author = "Margetić, Aleksandra and Stojanović, Sanja and Ristović, Marina and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "There is an urgent need to increase the daily intake of insoluble dietary fiber, and at the same time to find new sources and new production technologies. We hypothesized that fungal enzymes directly involved in lignocellulosic material hydrolysis (Aspergillus and Trichoderma enzyme cocktails) will change the fiber structure particularly efficiently after the action of laccase (Trametes versicolor enzyme cocktail). Enzymes production on an inducing substrate (same as starting material for obtainment of insoluble dietary fibers) and their usage resulted in obtainment of novel insoluble dietary fibers with better characteristics, 24% higher swelling, 43% higher WRC and 57% higher ORC compared to insoluble dietary fibers from triticale (already proven to be a good food additive). Changes in structure were analyzed by FTIR and microscopic analysis. Antioxidative performance of the obtained products, new insoluble and released soluble dietary fibers, was analyzed in detail. Newly obtained soluble dietary fibers demonstrated up to 20 times higher antioxidant activity compared to untreated fibers (ABTS and DPPH tests). These results suggest their good performance as a future food additive. At the same time, they prove the hypothesis that the use of enzyme cocktails rich in laccase is a good choice for biological pretreatment in this process.",
publisher = "Elsevier",
journal = "LWT - Food Science and Technology",
title = "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale",
volume = "145",
pages = "111291",
doi = "10.1016/j.lwt.2021.111291"
}

Margetić, A., Stojanović, S., Ristović, M., Vujčić, Z.,& Dojnov, B.. (2021). Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale. in LWT - Food Science and Technology
Elsevier., 145, 111291.
https://doi.org/10.1016/j.lwt.2021.111291

Margetić A, Stojanović S, Ristović M, Vujčić Z, Dojnov B. Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale. in LWT - Food Science and Technology. 2021;145:111291.
doi:10.1016/j.lwt.2021.111291 .

Margetić, Aleksandra, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, Dojnov, Biljana, "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale" in LWT - Food Science and Technology, 145 (2021):111291,
https://doi.org/10.1016/j.lwt.2021.111291 . .

TY  - DATA
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4541
AB  - FTIR spectra of IDFT treatment with fungal enzymatic complex.
PB  - Elsevier
T2  - LWT - Food Science and Technology
T1  - The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale"
UR  - https://hdl.handle.net/21.15107/rcub_cer_4541
ER  - 

@misc{
author = "Margetić, Aleksandra and Stojanović, Sanja and Ristović, Marina and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "FTIR spectra of IDFT treatment with fungal enzymatic complex.",
publisher = "Elsevier",
journal = "LWT - Food Science and Technology",
title = "The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale"",
url = "https://hdl.handle.net/21.15107/rcub_cer_4541"
}

Margetić, A., Stojanović, S., Ristović, M., Vujčić, Z.,& Dojnov, B.. (2021). The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale". in LWT - Food Science and Technology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cer_4541

Margetić A, Stojanović S, Ristović M, Vujčić Z, Dojnov B. The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale". in LWT - Food Science and Technology. 2021;.
https://hdl.handle.net/21.15107/rcub_cer_4541 .

Margetić, Aleksandra, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, Dojnov, Biljana, "The Supplementary data for: "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale"" in LWT - Food Science and Technology (2021),
https://hdl.handle.net/21.15107/rcub_cer_4541 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5815
AB  - Xylooligosaccharides (XOS) are prebiotic, functional food ingredients, with biological benefits
such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity.
Usually, XOS are produced from xylan by the combination of pre-treatment, enzymatic, chemical
and/or auto-hydrolytic methods. Fungal xylanases are the most suitable for XOS production. Xylan
rich agro-industrial wastes (corn cob is one of them) are used as a substrate for fungal xylanase
production and as start material for xylan extraction. In situ XOS production by fungal growth on
xylan rich medium represents an attractive and advantageous approach but insufficient described till
now. This method has many advantages over the others because bypasses the extraction and
purification of xylan and enzymes, it is environmentally friendly, low cost and time-consuming.
This study demonstrated A. tubingensis FAT35 has a great capacity for the synthesis of XOS using
corn cob as a substrate in solid state fermentation (SSF). Obtained XOS, during the fungal growth,
were characterized by TLC and HPLC. Significant antioxidant potential was shown by antioxidant
tests (ORAC, DPPH and FRAP). The obtained XOS are suitable to be a functional food additive
and are obtained in the simplest way that is both environmentally and economically suitable.
PB  - Belgrade : University of Belgrade
C3  - Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade
T1  - In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_cer_5815
ER  - 

@conference{
author = "Stojanović, Sanja and Ristović, Marina and Margetić, Aleksandra and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "Xylooligosaccharides (XOS) are prebiotic, functional food ingredients, with biological benefits
such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity.
Usually, XOS are produced from xylan by the combination of pre-treatment, enzymatic, chemical
and/or auto-hydrolytic methods. Fungal xylanases are the most suitable for XOS production. Xylan
rich agro-industrial wastes (corn cob is one of them) are used as a substrate for fungal xylanase
production and as start material for xylan extraction. In situ XOS production by fungal growth on
xylan rich medium represents an attractive and advantageous approach but insufficient described till
now. This method has many advantages over the others because bypasses the extraction and
purification of xylan and enzymes, it is environmentally friendly, low cost and time-consuming.
This study demonstrated A. tubingensis FAT35 has a great capacity for the synthesis of XOS using
corn cob as a substrate in solid state fermentation (SSF). Obtained XOS, during the fungal growth,
were characterized by TLC and HPLC. Significant antioxidant potential was shown by antioxidant
tests (ORAC, DPPH and FRAP). The obtained XOS are suitable to be a functional food additive
and are obtained in the simplest way that is both environmentally and economically suitable.",
publisher = "Belgrade : University of Belgrade",
journal = "Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade",
title = "In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_cer_5815"
}

Stojanović, S., Ristović, M., Margetić, A., Vujčić, Z.,& Dojnov, B.. (2021). In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade
Belgrade : University of Belgrade., 155.
https://hdl.handle.net/21.15107/rcub_cer_5815

Stojanović S, Ristović M, Margetić A, Vujčić Z, Dojnov B. In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob. in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade. 2021;:155.
https://hdl.handle.net/21.15107/rcub_cer_5815 .

Stojanović, Sanja, Ristović, Marina, Margetić, Aleksandra, Vujčić, Zoran, Dojnov, Biljana, "In situ production of xylooligosaccharides by Aspergillus tubingensis from corn cob" in Book of Abstracts - 2nd UNIFood International Conference - UNIFood2021, September 24-25, 2021, Belgrade (2021):155,
https://hdl.handle.net/21.15107/rcub_cer_5815 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Stepanović, Jelena
AU  - Margetić, Aleksandra
AU  - Duduk, Nataša
AU  - Dojnov, Biljana
AU  - Vujčić, Zoran
AU  - Duduk, Bojan
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5816
AB  - Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.
PB  - Sociedade Portuguesa de Química
C3  - Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line
T1  - Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate
SP  - 172
UR  - https://hdl.handle.net/21.15107/rcub_cer_5816
ER  - 

@conference{
author = "Stojanović, Sanja and Stepanović, Jelena and Margetić, Aleksandra and Duduk, Nataša and Dojnov, Biljana and Vujčić, Zoran and Duduk, Bojan",
year = "2021",
abstract = "Fructooligosaccharides (FOS) are used in the human diet thanks to their prebiotic effect and they are generally regarded as safe [1]. The increase in the functional food that contains prebiotics, has been tremendous over the last two decades. The most suitable process for FOS synthesis is by enzymes [2], particularly the one produced by filamentous fungi from the genus Aspergillus [2,3]. Aspergillus welwitschiae FAW1 strain, isolated from the environment, has proven to be non-toxigenic and safe for use in food production. Moreover, the absence of ochratoxins and fumonisins production capability was molecularly confirmed by the absence of complete or critical parts of biosynthetic gene clusters. Growing fungi on the natural substrate, triticale (Triticosecale sp), led to the production of various enzymes from inulinase complex (InuA, InuE, FTase, FFase). Production of FOS has been tested in two possible ways with the obtained inulinase enzyme complex: (1) synthesis from sucrose – fructosyltransferase activity and (2) hydrolysis of inulin – endoinulinase and exoinulinase activity. The obtained FOS were detected by TLC and HPLC methods and characterized by examination of antioxidant capacity. Produced FOS showed significant antioxidant potential according to ABTS and ORAC which classifies them as important additives in functional food. These findings open up opportunities for an easy approach for FOS production by fungal inulinase enzymes, without their prior separation and purification.",
publisher = "Sociedade Portuguesa de Química",
journal = "Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line",
title = "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate",
pages = "172",
url = "https://hdl.handle.net/21.15107/rcub_cer_5816"
}

Stojanović, S., Stepanović, J., Margetić, A., Duduk, N., Dojnov, B., Vujčić, Z.,& Duduk, B.. (2021). Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line
Sociedade Portuguesa de Química., 172.
https://hdl.handle.net/21.15107/rcub_cer_5816

Stojanović S, Stepanović J, Margetić A, Duduk N, Dojnov B, Vujčić Z, Duduk B. Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate. in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line. 2021;:172.
https://hdl.handle.net/21.15107/rcub_cer_5816 .

Stojanović, Sanja, Stepanović, Jelena, Margetić, Aleksandra, Duduk, Nataša, Dojnov, Biljana, Vujčić, Zoran, Duduk, Bojan, "Production of fructooligosaccharides by Aspergillus welwitschiae inulinase enzyme complex, obtained on natural substrate" in Book of abstracts - XXI EuroFoodChem Conference, 22-24 November 2021, on-line (2021):172,
https://hdl.handle.net/21.15107/rcub_cer_5816 .

TY  - CONF
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5818
AB  - Wild-type microorganisms from the environment represent a wide source of potential 
enzyme producers. In order to determine whether an isolated microorganism produces an 
enzyme of interest, various screen tests have been developed. A new screening method 
for detection of endo and exo-xylanase activity including short time growth of fungal 
strains on a minimal medium containing xylan (inducible substrate) as a carbon source is 
developed and used for testing 58 fungal isolates from genus Aspergillus. The test is based 
on the diffusion of samples (fermentation extracts) in polyacrylamide gel incorporated by 
xylan. Endoxylanase activity is detected as enlightenment in the gel after staining of xylan 
with Congo Red. Exoxylanase activity was visualized as a precipitate after staining of 
reduction oligosaccharide ends with NBT. Selected isolate A. tubigensis was grown on 
SSF where corn cob served as an inducible substrate. In order to examine the influence of 
nitrogen sources on endoxylanase production and fungal growth, two sources (peptone and 
urea) were varied in 3 concentrations (1, 5 and 10 g/L). There were statistically significant 
differences in the obtained activities. The increase in activity compared to the screening 
medium was ~250 times. The obtained enzymes with high specific activity were further 
used for the production of xylooligosaccharides in high yield which showed that the 
selection of strain A. tubingensis was good.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production
SP  - 138
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_cer_5818
ER  - 

@conference{
author = "Ristović, Marina and Stojanović, Sanja and Margetić, Aleksandra and Vujčić, Zoran and Dojnov, Biljana",
year = "2021",
abstract = "Wild-type microorganisms from the environment represent a wide source of potential 
enzyme producers. In order to determine whether an isolated microorganism produces an 
enzyme of interest, various screen tests have been developed. A new screening method 
for detection of endo and exo-xylanase activity including short time growth of fungal 
strains on a minimal medium containing xylan (inducible substrate) as a carbon source is 
developed and used for testing 58 fungal isolates from genus Aspergillus. The test is based 
on the diffusion of samples (fermentation extracts) in polyacrylamide gel incorporated by 
xylan. Endoxylanase activity is detected as enlightenment in the gel after staining of xylan 
with Congo Red. Exoxylanase activity was visualized as a precipitate after staining of 
reduction oligosaccharide ends with NBT. Selected isolate A. tubigensis was grown on 
SSF where corn cob served as an inducible substrate. In order to examine the influence of 
nitrogen sources on endoxylanase production and fungal growth, two sources (peptone and 
urea) were varied in 3 concentrations (1, 5 and 10 g/L). There were statistically significant 
differences in the obtained activities. The increase in activity compared to the screening 
medium was ~250 times. The obtained enzymes with high specific activity were further 
used for the production of xylooligosaccharides in high yield which showed that the 
selection of strain A. tubingensis was good.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production",
pages = "138-139",
url = "https://hdl.handle.net/21.15107/rcub_cer_5818"
}

Ristović, M., Stojanović, S., Margetić, A., Vujčić, Z.,& Dojnov, B.. (2021). Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 138-139.
https://hdl.handle.net/21.15107/rcub_cer_5818

Ristović M, Stojanović S, Margetić A, Vujčić Z, Dojnov B. Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:138-139.
https://hdl.handle.net/21.15107/rcub_cer_5818 .

Ristović, Marina, Stojanović, Sanja, Margetić, Aleksandra, Vujčić, Zoran, Dojnov, Biljana, "Diffusion screening method for estimation potential fungal producers of xylanase responsible for xylooligosaccharides production" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):138-139,
https://hdl.handle.net/21.15107/rcub_cer_5818 .

TY  - CONF
AU  - Ralić, Vanja
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5835
AB  - Polyglutamic acid (PGA) is an anionic, non-toxic natural polymer that consists of D- and L-glutamic acid. Glutamic acids that makeup PGA create bonds between α-amino and γ-carboxyl groups. PGA can be found on the surface of many different bacteria, usually as a part of their capsule. Bacteria use polyglutamic acid to survive adverse environmental conditions. PGA has found multiple potential applications as a thickener, drug carrier, biological adhesive, heavy metal and basic dye adsorber, etc. Its biodegradability is especially useful in the fields of food, cosmetics, medicine and water treatment1. The aim of this study was to find the best polyglutamic acid producer from the selection of 50 different Bacillus sp strains originating from Serbia, as well as the optimal medium composition. It was discovered that the best PGA producing strain was 17B and it was selected for further fermentation medium optimization. Optimization was carried out using Design of Experiment, specifically Box Behnken design. Results were analyzed using response surface methodology. PGA that was produced during this process was analyzed using SDS PAGE and basic dye adsorption was attempted as well. The best PGA producer, Bacillus sp strain 17B, was used for PGA production in batch fermenter 2. PGA isolated from the fermentation broth was purified using gel filtration and further characterized using SDS PAGE, FTIR spectroscopy and direct-infusion MS.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
T1  - Production of polyglutamic acid by Bacillus sp: Strains selection, optimization, batch fermentation and characterization
SP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_cer_5835
ER  - 

@conference{
author = "Ralić, Vanja and Šokarda Slavić, Marinela and Margetić, Aleksandra and Vujčić, Zoran",
year = "2021",
abstract = "Polyglutamic acid (PGA) is an anionic, non-toxic natural polymer that consists of D- and L-glutamic acid. Glutamic acids that makeup PGA create bonds between α-amino and γ-carboxyl groups. PGA can be found on the surface of many different bacteria, usually as a part of their capsule. Bacteria use polyglutamic acid to survive adverse environmental conditions. PGA has found multiple potential applications as a thickener, drug carrier, biological adhesive, heavy metal and basic dye adsorber, etc. Its biodegradability is especially useful in the fields of food, cosmetics, medicine and water treatment1. The aim of this study was to find the best polyglutamic acid producer from the selection of 50 different Bacillus sp strains originating from Serbia, as well as the optimal medium composition. It was discovered that the best PGA producing strain was 17B and it was selected for further fermentation medium optimization. Optimization was carried out using Design of Experiment, specifically Box Behnken design. Results were analyzed using response surface methodology. PGA that was produced during this process was analyzed using SDS PAGE and basic dye adsorption was attempted as well. The best PGA producer, Bacillus sp strain 17B, was used for PGA production in batch fermenter 2. PGA isolated from the fermentation broth was purified using gel filtration and further characterized using SDS PAGE, FTIR spectroscopy and direct-infusion MS.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia",
title = "Production of polyglutamic acid by Bacillus sp: Strains selection, optimization, batch fermentation and characterization",
pages = "157",
url = "https://hdl.handle.net/21.15107/rcub_cer_5835"
}

Ralić, V., Šokarda Slavić, M., Margetić, A.,& Vujčić, Z.. (2021). Production of polyglutamic acid by Bacillus sp: Strains selection, optimization, batch fermentation and characterization. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia
University of Belgrade - Faculty of Chemistry., 157.
https://hdl.handle.net/21.15107/rcub_cer_5835

Ralić V, Šokarda Slavić M, Margetić A, Vujčić Z. Production of polyglutamic acid by Bacillus sp: Strains selection, optimization, batch fermentation and characterization. in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia. 2021;:157.
https://hdl.handle.net/21.15107/rcub_cer_5835 .

Ralić, Vanja, Šokarda Slavić, Marinela, Margetić, Aleksandra, Vujčić, Zoran, "Production of polyglutamic acid by Bacillus sp: Strains selection, optimization, batch fermentation and characterization" in Proceedings - X Conference of Serbian Biochemical Society with international participation, “Biochemical Insights into Molecular Mechanisms”, 24.09.2021. Kragujevac, Serbia (2021):157,
https://hdl.handle.net/21.15107/rcub_cer_5835 .