Stanisavljević, Nemanja

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orcid::0000-0001-9484-1166
  • Stanisavljević, Nemanja (3)
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Author's Bibliography

Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications

Šokarda Slavić, Marinela; Kojić, Milan; Margetić, Aleksandra; Stanisavljević, Nemanja; Gardijan, Lazar; Božić, Nataša; Vujčić, Zoran

(Elsevier, 2023)

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Stanisavljević, Nemanja
AU  - Gardijan, Lazar
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6458
AB  - α-Amylase from the thermophilic bacterial strain Anoxybacillus vranjensis ST4 (AVA) was cloned into the pMALc5HisEk expression vector and successfully expressed and purified from the Escherichia coli ER2523 host strain. AVA belongs to the GH13_5 subfamily of glycoside hydrolases and has 7 conserved sequence regions (CSRs) distributed in three distinct domains (A, B, C). In addition, there is a starch binding domain (SBD) from the CBM20 family of carbohydrate binding modules (CBMs). AVA is a monomer of 66 kDa that achieves maximum activity at 60–80 °C and is active and stable over a wide pH range (4.0–9.0). AVA retained 50 % of its activity after 31 h of incubation at 60 °C and was resistant to a large number of denaturing agents. It hydrolyzed starch granules very efficiently, releasing maltose, maltotriose and maltopentaose as the main products. The hydrolysis rates of raw corn, wheat, horseradish, and potato starch, at a concentration of 10 %, were 87.8, 85.9, 93.0, and 58 %, respectively, at pH 8.5 over a 3 h period. This study showed that the high level of expression as well as the properties of this highly stable and versatile enzyme show all the prerequisites for successful application in industry.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications
VL  - 249
SP  - 126055
DO  - 10.1016/j.ijbiomac.2023.126055
ER  - 
@article{
author = "Šokarda Slavić, Marinela and Kojić, Milan and Margetić, Aleksandra and Stanisavljević, Nemanja and Gardijan, Lazar and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "α-Amylase from the thermophilic bacterial strain Anoxybacillus vranjensis ST4 (AVA) was cloned into the pMALc5HisEk expression vector and successfully expressed and purified from the Escherichia coli ER2523 host strain. AVA belongs to the GH13_5 subfamily of glycoside hydrolases and has 7 conserved sequence regions (CSRs) distributed in three distinct domains (A, B, C). In addition, there is a starch binding domain (SBD) from the CBM20 family of carbohydrate binding modules (CBMs). AVA is a monomer of 66 kDa that achieves maximum activity at 60–80 °C and is active and stable over a wide pH range (4.0–9.0). AVA retained 50 % of its activity after 31 h of incubation at 60 °C and was resistant to a large number of denaturing agents. It hydrolyzed starch granules very efficiently, releasing maltose, maltotriose and maltopentaose as the main products. The hydrolysis rates of raw corn, wheat, horseradish, and potato starch, at a concentration of 10 %, were 87.8, 85.9, 93.0, and 58 %, respectively, at pH 8.5 over a 3 h period. This study showed that the high level of expression as well as the properties of this highly stable and versatile enzyme show all the prerequisites for successful application in industry.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications",
volume = "249",
pages = "126055",
doi = "10.1016/j.ijbiomac.2023.126055"
}
Šokarda Slavić, M., Kojić, M., Margetić, A., Stanisavljević, N., Gardijan, L., Božić, N.,& Vujčić, Z.. (2023). Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications. in International Journal of Biological Macromolecules
Elsevier., 249, 126055.
https://doi.org/10.1016/j.ijbiomac.2023.126055
Šokarda Slavić M, Kojić M, Margetić A, Stanisavljević N, Gardijan L, Božić N, Vujčić Z. Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications. in International Journal of Biological Macromolecules. 2023;249:126055.
doi:10.1016/j.ijbiomac.2023.126055 .
Šokarda Slavić, Marinela, Kojić, Milan, Margetić, Aleksandra, Stanisavljević, Nemanja, Gardijan, Lazar, Božić, Nataša, Vujčić, Zoran, "Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications" in International Journal of Biological Macromolecules, 249 (2023):126055,
https://doi.org/10.1016/j.ijbiomac.2023.126055 . .
1

Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.

Tomić, Katarina; Šokarda Slavić, Marinela; Kojić, Milan; Stanisavljević, Nemanja; Nikolić, Stefan; Vujčić, Zoran

(University of Belgrade - Faculty of Chemistry, 2022)

TY  - CONF
AU  - Tomić, Katarina
AU  - Šokarda Slavić, Marinela
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5917
AB  - One of the most abundant natural polymers with multidimensional and multifaceted application is starch. Due to energy fuel sustainability concern, the world is focusing on renewable energy including energy from renewable biological materials like starch1. The importance of the enzymatic hydrolysis of granular starch below the temperature of gelatinization has been well recognized, mainly due to energy savings and the effective utilization of biomass, which reduces the overall cost of starch processing2. A new α-amylase gene (Amy35) was cloned from newly isolated thermophilic Anoxybacillus sp. ST4 and expressed in Escherichia coli. The purified recombinant α-amylase had an wide pH optimum range from 4.5 to 8.5 and optimum temperature of 75°C. The enzyme retained 95% of its activity after 3h of incubation at 50 and 60°C. Hydrolysis rates of potato, horseradish and corn starches, at 1% concentration were 20, 70 and 65%, respectively, in a period of 16 h. Analysis of the enzyme properties proved its high efficacy for the digestion of diverse raw starches below gelatinization temperature and, therefore, its potential commercial value for use as an industrial enzyme.
PB  - University of Belgrade - Faculty of Chemistry
PB  - Serbian Biochemical Society
C3  - Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
T1  - Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_cer_5917
ER  - 
@conference{
author = "Tomić, Katarina and Šokarda Slavić, Marinela and Kojić, Milan and Stanisavljević, Nemanja and Nikolić, Stefan and Vujčić, Zoran",
year = "2022",
abstract = "One of the most abundant natural polymers with multidimensional and multifaceted application is starch. Due to energy fuel sustainability concern, the world is focusing on renewable energy including energy from renewable biological materials like starch1. The importance of the enzymatic hydrolysis of granular starch below the temperature of gelatinization has been well recognized, mainly due to energy savings and the effective utilization of biomass, which reduces the overall cost of starch processing2. A new α-amylase gene (Amy35) was cloned from newly isolated thermophilic Anoxybacillus sp. ST4 and expressed in Escherichia coli. The purified recombinant α-amylase had an wide pH optimum range from 4.5 to 8.5 and optimum temperature of 75°C. The enzyme retained 95% of its activity after 3h of incubation at 50 and 60°C. Hydrolysis rates of potato, horseradish and corn starches, at 1% concentration were 20, 70 and 65%, respectively, in a period of 16 h. Analysis of the enzyme properties proved its high efficacy for the digestion of diverse raw starches below gelatinization temperature and, therefore, its potential commercial value for use as an industrial enzyme.",
publisher = "University of Belgrade - Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia",
title = "Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_cer_5917"
}
Tomić, K., Šokarda Slavić, M., Kojić, M., Stanisavljević, N., Nikolić, S.,& Vujčić, Z.. (2022). Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia
University of Belgrade - Faculty of Chemistry., 147.
https://hdl.handle.net/21.15107/rcub_cer_5917
Tomić K, Šokarda Slavić M, Kojić M, Stanisavljević N, Nikolić S, Vujčić Z. Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp.. in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia. 2022;:147.
https://hdl.handle.net/21.15107/rcub_cer_5917 .
Tomić, Katarina, Šokarda Slavić, Marinela, Kojić, Milan, Stanisavljević, Nemanja, Nikolić, Stefan, Vujčić, Zoran, "Cloning and characterization of new raw starch digestion α-amylase from thermophilic Anoxybacillus sp." in Proceedings - XI Conference of Serbian Biochemical Society "Amazing Biochemistry", 22.09.2022. Novi Sad, Serbia (2022):147,
https://hdl.handle.net/21.15107/rcub_cer_5917 .

Analysis of Efficacy of the Process of Remediation of Landfill Site Soil Contaminated with Leachate using Material Flow Analysis

Antić, Katarina Đ.; Tošić, Nikolina; Turk Sekulić, Maja; Radonić, Jelena; Stanisavljević, Nemanja

(Univerzitet u Beogradu - Tehnički fakultet u Boru, 2018)

TY  - JOUR
AU  - Antić, Katarina Đ.
AU  - Tošić, Nikolina
AU  - Turk Sekulić, Maja
AU  - Radonić, Jelena
AU  - Stanisavljević, Nemanja
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6098
AB  - There are two possible cases concerning non-sanitary municipal solid waste landfills due to failure to meet requirements with respect to impermeability and thickness of geological barrier as well as absence of implementation and use of a waste and leachate management system, and also depending on type and the mineral composition of the geological barrier. In the first case, impermeable clay, kaolinite, is prevailing in the composition of the geological barrier the final outcome of which is the spillage of leachate into the volume of the deposited waste, as well as its outbreak on the surface of the landfill site. In the second case, permeable clay, smectite, is prevailing in the composition of geological barrier with dominant leachate infiltration into the ground flow. In accordance with the first case, using STAN software the substance flow analysis (SFA) was carried out, of cadmium primarily, within three scenarios. Efficacy of remediation of the landfill site soil contaminated with leachate from the aspect of heavy metal reduction, focused primarily on cadmium, using in-situ and ex-situ systems, was presented within Scenario 1 and Scenario 2. Possibility and efficacy of direct treatment of leachate in a corresponding plant for its processing, in case of implementing and using the leachate management system, was presented within Scenario 3. Efficacy of cadmium flow reduction accomplished within Scenario 1, Scenario 2 and Scenario 3 amounts to 99.04 %, 55 % and 99.2 %, respectively. Prevention of landfill site contamination with leachate using the system of its management is an optimum option and a desirable practice, while in the case of the already existing contamination of the landfill site and its immediate environment, using in-situ system of landfill site soil remediation, based on achieved results, high efficacy was achieved contrary to use of the ex-situ system.
AB  - Kod nesanitarnih komunalnih deponija čvrstog otpada, usled nezadovoljavanja zahteva u pogledu vodonepropusnosti i debljine geološke barijere i odsustva implementacije i primene sistema za upravljanje otpadom i procednim vodama, kao i u zavisnosti od vrste i minerološkog sastava geološke barijere, moguća su dva slučaja. Pri prvom slučaju, u okviru sastava geološke barijere dominira nepropusna glina, kaolinit, te krajnji ishod predstavlja izlivanje procednih voda u zapreminu deponovanog otpada, kao i izbijanje istih na površinu deponijskog lokaliteta. Pri drugom slučaju, propusne gline, smektiti, preovlađuju u okviru sastava geološke barijere, te je dominantna infiltracija procednih voda u podzemni tok. U skladu sa prvim slučajem, primenom softvera STAN realizovana je analiza tokova supstanci (SFA), primarno kadmijuma, u okviru tri scenarija. Efikasnost remedijacije zemljišta deponijskog lokaliteta kontaminiranog procednim vodama, sa aspekta redukcije teških metala, sa akcentom na kadmijum, primenom in-situ i ex-situ sistema, predstavljena je u okviru Scenarija 1 i Scenarija 2. Mogućnost i efikasnost direktnog tretmana procednih voda u postrojenju za tretman istih, u slučaju implementacije i primene sistema za upravljanje procednim vodama, predstavljena je u okviru Scenarija 3. Efikasnost redukcije toka kadmijuma ostvarena u okviru Scenarija 1 iznosi 99.04 %, u okviru Scenarija 2 iznosi 55 %, dok u okviru Scenarija 3 iznosi 99.2 %, respektivno. Prevencija kontaminacije deponijskog lokaliteta procednim vodama primenom sistema za upravljanje istim predstavlja optimalnu opciju i poželjnu praksu, dok u slučaju već postojeće kontaminacije deponijskog lokaliteta i neposredne okoline, primenom in-situ sistema remedijacije zemljišta deponijskog lokaliteta, na osnovu dobijenih rezultata, ostvaruje se visoka efikasnost, nasuprot primeni ex-situ sistema.
PB  - Univerzitet u Beogradu - Tehnički fakultet u Boru
T2  - Recycling and Sustainable Development (RSD) / Reciklaža i održivi razvoj
T1  - Analysis of Efficacy of the Process of Remediation of Landfill Site Soil Contaminated with Leachate using Material Flow Analysis
T1  - Analiza efikasnosti procesa remedijacije zemljišta deponijskog lokaliteta kontaminiranog procednim vodama primenom analize tokova materijala
VL  - 11
IS  - 1
SP  - 27
EP  - 42
DO  - 10.5937/ror1801027A
ER  - 
@article{
author = "Antić, Katarina Đ. and Tošić, Nikolina and Turk Sekulić, Maja and Radonić, Jelena and Stanisavljević, Nemanja",
year = "2018",
abstract = "There are two possible cases concerning non-sanitary municipal solid waste landfills due to failure to meet requirements with respect to impermeability and thickness of geological barrier as well as absence of implementation and use of a waste and leachate management system, and also depending on type and the mineral composition of the geological barrier. In the first case, impermeable clay, kaolinite, is prevailing in the composition of the geological barrier the final outcome of which is the spillage of leachate into the volume of the deposited waste, as well as its outbreak on the surface of the landfill site. In the second case, permeable clay, smectite, is prevailing in the composition of geological barrier with dominant leachate infiltration into the ground flow. In accordance with the first case, using STAN software the substance flow analysis (SFA) was carried out, of cadmium primarily, within three scenarios. Efficacy of remediation of the landfill site soil contaminated with leachate from the aspect of heavy metal reduction, focused primarily on cadmium, using in-situ and ex-situ systems, was presented within Scenario 1 and Scenario 2. Possibility and efficacy of direct treatment of leachate in a corresponding plant for its processing, in case of implementing and using the leachate management system, was presented within Scenario 3. Efficacy of cadmium flow reduction accomplished within Scenario 1, Scenario 2 and Scenario 3 amounts to 99.04 %, 55 % and 99.2 %, respectively. Prevention of landfill site contamination with leachate using the system of its management is an optimum option and a desirable practice, while in the case of the already existing contamination of the landfill site and its immediate environment, using in-situ system of landfill site soil remediation, based on achieved results, high efficacy was achieved contrary to use of the ex-situ system., Kod nesanitarnih komunalnih deponija čvrstog otpada, usled nezadovoljavanja zahteva u pogledu vodonepropusnosti i debljine geološke barijere i odsustva implementacije i primene sistema za upravljanje otpadom i procednim vodama, kao i u zavisnosti od vrste i minerološkog sastava geološke barijere, moguća su dva slučaja. Pri prvom slučaju, u okviru sastava geološke barijere dominira nepropusna glina, kaolinit, te krajnji ishod predstavlja izlivanje procednih voda u zapreminu deponovanog otpada, kao i izbijanje istih na površinu deponijskog lokaliteta. Pri drugom slučaju, propusne gline, smektiti, preovlađuju u okviru sastava geološke barijere, te je dominantna infiltracija procednih voda u podzemni tok. U skladu sa prvim slučajem, primenom softvera STAN realizovana je analiza tokova supstanci (SFA), primarno kadmijuma, u okviru tri scenarija. Efikasnost remedijacije zemljišta deponijskog lokaliteta kontaminiranog procednim vodama, sa aspekta redukcije teških metala, sa akcentom na kadmijum, primenom in-situ i ex-situ sistema, predstavljena je u okviru Scenarija 1 i Scenarija 2. Mogućnost i efikasnost direktnog tretmana procednih voda u postrojenju za tretman istih, u slučaju implementacije i primene sistema za upravljanje procednim vodama, predstavljena je u okviru Scenarija 3. Efikasnost redukcije toka kadmijuma ostvarena u okviru Scenarija 1 iznosi 99.04 %, u okviru Scenarija 2 iznosi 55 %, dok u okviru Scenarija 3 iznosi 99.2 %, respektivno. Prevencija kontaminacije deponijskog lokaliteta procednim vodama primenom sistema za upravljanje istim predstavlja optimalnu opciju i poželjnu praksu, dok u slučaju već postojeće kontaminacije deponijskog lokaliteta i neposredne okoline, primenom in-situ sistema remedijacije zemljišta deponijskog lokaliteta, na osnovu dobijenih rezultata, ostvaruje se visoka efikasnost, nasuprot primeni ex-situ sistema.",
publisher = "Univerzitet u Beogradu - Tehnički fakultet u Boru",
journal = "Recycling and Sustainable Development (RSD) / Reciklaža i održivi razvoj",
title = "Analysis of Efficacy of the Process of Remediation of Landfill Site Soil Contaminated with Leachate using Material Flow Analysis, Analiza efikasnosti procesa remedijacije zemljišta deponijskog lokaliteta kontaminiranog procednim vodama primenom analize tokova materijala",
volume = "11",
number = "1",
pages = "27-42",
doi = "10.5937/ror1801027A"
}
Antić, K. Đ., Tošić, N., Turk Sekulić, M., Radonić, J.,& Stanisavljević, N.. (2018). Analysis of Efficacy of the Process of Remediation of Landfill Site Soil Contaminated with Leachate using Material Flow Analysis. in Recycling and Sustainable Development (RSD) / Reciklaža i održivi razvoj
Univerzitet u Beogradu - Tehnički fakultet u Boru., 11(1), 27-42.
https://doi.org/10.5937/ror1801027A
Antić KĐ, Tošić N, Turk Sekulić M, Radonić J, Stanisavljević N. Analysis of Efficacy of the Process of Remediation of Landfill Site Soil Contaminated with Leachate using Material Flow Analysis. in Recycling and Sustainable Development (RSD) / Reciklaža i održivi razvoj. 2018;11(1):27-42.
doi:10.5937/ror1801027A .
Antić, Katarina Đ., Tošić, Nikolina, Turk Sekulić, Maja, Radonić, Jelena, Stanisavljević, Nemanja, "Analysis of Efficacy of the Process of Remediation of Landfill Site Soil Contaminated with Leachate using Material Flow Analysis" in Recycling and Sustainable Development (RSD) / Reciklaža i održivi razvoj, 11, no. 1 (2018):27-42,
https://doi.org/10.5937/ror1801027A . .