TY  - JOUR
AU  - Tasić, Ljubica
AU  - Avramović, Nataša
AU  - Quintero, Melissa
AU  - Stanišić, Danijela
AU  - Martins, Lucas
AU  - Brena da Costa, Tássia
AU  - Jadranin, Milka
AU  - Accioly de Souza, Maria Theresa
AU  - Faria, Paulo
AU  - de Camargo, Beatriz
AU  - de Sá Pereira, Bruna Maria
AU  - Maschietto, Mariana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5323
AB  - Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.
PB  - MDPI
T2  - Diagnostic
T1  - A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy
VL  - 12
IS  - 1
SP  - 2
EP  - 10
DO  - 10.3390/diagnostics12010157
ER  - 

@article{
author = "Tasić, Ljubica and Avramović, Nataša and Quintero, Melissa and Stanišić, Danijela and Martins, Lucas and Brena da Costa, Tássia and Jadranin, Milka and Accioly de Souza, Maria Theresa and Faria, Paulo and de Camargo, Beatriz and de Sá Pereira, Bruna Maria and Maschietto, Mariana",
year = "2022",
abstract = "Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.",
publisher = "MDPI",
journal = "Diagnostic",
title = "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy",
volume = "12",
number = "1",
pages = "2-10",
doi = "10.3390/diagnostics12010157"
}

Tasić, L., Avramović, N., Quintero, M., Stanišić, D., Martins, L., Brena da Costa, T., Jadranin, M., Accioly de Souza, M. T., Faria, P., de Camargo, B., de Sá Pereira, B. M.,& Maschietto, M.. (2022). A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic
MDPI., 12(1), 2-10.
https://doi.org/10.3390/diagnostics12010157

Tasić L, Avramović N, Quintero M, Stanišić D, Martins L, Brena da Costa T, Jadranin M, Accioly de Souza MT, Faria P, de Camargo B, de Sá Pereira BM, Maschietto M. A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic. 2022;12(1):2-10.
doi:10.3390/diagnostics12010157 .

Tasić, Ljubica, Avramović, Nataša, Quintero, Melissa, Stanišić, Danijela, Martins, Lucas, Brena da Costa, Tássia, Jadranin, Milka, Accioly de Souza, Maria Theresa, Faria, Paulo, de Camargo, Beatriz, de Sá Pereira, Bruna Maria, Maschietto, Mariana, "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy" in Diagnostic, 12, no. 1 (2022):2-10,
https://doi.org/10.3390/diagnostics12010157 . .

TY  - CONF
AU  - Tasić, Ljubica
AU  - Martins, Lucas
AU  - Braga, Erik Sobrinho
AU  - Novais, M.
AU  - Jadranin, Milka
AU  - Stanišić, Danijela
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7277
AB  - The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.
PB  - NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)
C3  - 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
T1  - Impressions on COVID-19 by NMR
SP  - OC24
UR  - https://hdl.handle.net/21.15107/rcub_cer_7277
ER  - 

@conference{
author = "Tasić, Ljubica and Martins, Lucas and Braga, Erik Sobrinho and Novais, M. and Jadranin, Milka and Stanišić, Danijela",
year = "2021",
abstract = "The infection with SARS-CoV-2 mostly leads to mild disease, but some individuals progress to severe disease with death outcome [1]. These severe cases are driven by the host response to the infection, with multisystem dysfunctions [1,2]. Timely intervention in patients in critical conditions could be crucial for their survival. Therefore, it is of paramount importance to understand the disease phase and its progression markers. Serum samples from two cohorts of patients, with mild (n = 92) and severe disease (n = 41) were analyzed, with two subgroups in each cohort, positive and negative to the virus. The samples were age and gender-matched to healthy volunteers (n = 87), too. Three NMR data sets (1H-NMR, T2, and edited by diffusion) were recorded on a 600 MHz Bruker AVANCE III spectrometer, and analyzed in MetaboAnalyst 5.0. Serum metabolomics showed that proteins, very low and low-density lipoproteins (VLDL and LDL), phospholipids, 
lysophosphatidylcholines among other 50 metabolites were disrupted. Furthermore, changes in the levels of NAD(P)+, hyperglycemia and dysregulated glucose were detrimental in people with COVID-19. Glu cose excess, increased levels of VLDL, and decreased proteins were related to the disease severity, and might be important for determining a patient’s phase of the disease, and NMR can greatly add to the differentiation between severe and mild symptoms.",
publisher = "NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)",
journal = "10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event",
title = "Impressions on COVID-19 by NMR",
pages = "OC24",
url = "https://hdl.handle.net/21.15107/rcub_cer_7277"
}

Tasić, L., Martins, L., Braga, E. S., Novais, M., Jadranin, M.,& Stanišić, D.. (2021). Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event
NMR Group (GERMN) of the Spanish Royal Society of Chemistry (RSEQ)., OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277

Tasić L, Martins L, Braga ES, Novais M, Jadranin M, Stanišić D. Impressions on COVID-19 by NMR. in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event. 2021;:OC24.
https://hdl.handle.net/21.15107/rcub_cer_7277 .

Tasić, Ljubica, Martins, Lucas, Braga, Erik Sobrinho, Novais, M., Jadranin, Milka, Stanišić, Danijela, "Impressions on COVID-19 by NMR" in 10th GERMN biennial NMR meeting, 9th IberoAmerican NMR meeting, 7th Iberian NMR meeting, 26 to 29 April 2021, virtual event (2021):OC24,
https://hdl.handle.net/21.15107/rcub_cer_7277 .

TY  - CONF
AU  - Stanišić, Danijela
AU  - Jadranin, Milka
AU  - Tasić, Ljubica
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7278
AB  - Identificar metabólitos séricos que refletem o estado e a gravidade da doença pode ajudar no diagnóstico e prognóstico, definir as melhores intervenções a serem aplicadas e o momento correto, bem como compreender o impacto da doença em todo o corpo. Com isso em mente, este estudo se propôs a medir o metabolôma do soro sanguíneo de 37 pacientes com sintomas de COVID-19 grave usando RMN de 1H. Os pacientes que foram positivos para SARS-CoV-2 por RT-qPCR foram agrupados como “COVID-19 grave” (P, vermelho, n = 21, Fig. 1), enquanto os pacientes negativos por RT-qPCR foram agrupados como “não COVID-19 grave” (N, azul, n = 16, Fig. 1). Essas amostras foram comparadas com as amostras de referência de voluntários 
saudáveis (H, verde, n = 40, Fig. 1) retiradas antes de início da pandemia. Os grupos eram homogêneos em relação ao gênero, idade e comorbidades para evitar qualquer comparação tendenciosa. Os soros (200 µL) foram descongelados em gelo, misturados com 250 µL de óxido de deutério (D2O 99,9%) em temperatura ambiente, centrifugados a 12,000 g por 2 min a 4 ° C e transferidos para tubos de RMN de 5 mm. Os metabólitos não polares (lipídios) foram isolados e analisados usando CDCl3 como solvente. Os espectros de alta resolução de RMN de 1H NOESY 1D, CPMG, DOSY editado e em 2D (TOCSY, HSQC) foram adquiridos no espectrômetro Bruker AVANCE III 600 MHz usando a sonda tripla inversa (TBI) a 25 °C. Os três grupos (P, N e H) foram 
separados com facilidade em PCA e em PLSDA (Fig. 1) independentemente da matriz de dados usada. Os dados de RMN de 1H de NOESY, CPMG, editados por DOSY do soro ou de lipídios isolados acusaram diferenças evidentes entre os grupos analisados. Os efeitos da COVID-19 sobre metabólitos séricos foram extremos e refletem todas as respostas do corpo ao vírus SARS-CoV-2. Especialmente os altos níveis de glicose chamam a atenção e a desproporção em níveis e tipos de lipídios. Enquanto o colesterol e os esteróis livres foram muito menores em pacientes com COVID-19 grave do que em controles saudáveis, as fosfocolinas e VLDL foram as mais abundantes. Além disso, a maioria (60-75%) dos metabólitos do soro sanguíneo aumentou em 
pacientes com COVID-19 e em pacientes que foram negativos, porém com sintomas graves. Quando analisadas as vias metabólicas, muitas delas foram alteradas de maneira marcante devido à doença. Como por exemplo a biossíntese de aminoacil t-RNA, metabolismo de aminoácidos (glicina, serina e treonina), metabolismo de aminoácidos de cadeia ramificada (valina, leucina e isoleucina), metabolismo de glicerofosfolipídeos, entre outras vias. Finalmente, a RMN de 1H poderá ser usada com sucesso para classificar os pacientes graves devido múltiplas e várias alterações de metabólitos e níveis de lipídios no seu soro.
PB  - Associação de Usuários de RMN - AUREMN
C3  - XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online
T1  - Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN
UR  - https://hdl.handle.net/21.15107/rcub_cer_7278
ER  - 

@conference{
author = "Stanišić, Danijela and Jadranin, Milka and Tasić, Ljubica",
year = "2020",
abstract = "Identificar metabólitos séricos que refletem o estado e a gravidade da doença pode ajudar no diagnóstico e prognóstico, definir as melhores intervenções a serem aplicadas e o momento correto, bem como compreender o impacto da doença em todo o corpo. Com isso em mente, este estudo se propôs a medir o metabolôma do soro sanguíneo de 37 pacientes com sintomas de COVID-19 grave usando RMN de 1H. Os pacientes que foram positivos para SARS-CoV-2 por RT-qPCR foram agrupados como “COVID-19 grave” (P, vermelho, n = 21, Fig. 1), enquanto os pacientes negativos por RT-qPCR foram agrupados como “não COVID-19 grave” (N, azul, n = 16, Fig. 1). Essas amostras foram comparadas com as amostras de referência de voluntários 
saudáveis (H, verde, n = 40, Fig. 1) retiradas antes de início da pandemia. Os grupos eram homogêneos em relação ao gênero, idade e comorbidades para evitar qualquer comparação tendenciosa. Os soros (200 µL) foram descongelados em gelo, misturados com 250 µL de óxido de deutério (D2O 99,9%) em temperatura ambiente, centrifugados a 12,000 g por 2 min a 4 ° C e transferidos para tubos de RMN de 5 mm. Os metabólitos não polares (lipídios) foram isolados e analisados usando CDCl3 como solvente. Os espectros de alta resolução de RMN de 1H NOESY 1D, CPMG, DOSY editado e em 2D (TOCSY, HSQC) foram adquiridos no espectrômetro Bruker AVANCE III 600 MHz usando a sonda tripla inversa (TBI) a 25 °C. Os três grupos (P, N e H) foram 
separados com facilidade em PCA e em PLSDA (Fig. 1) independentemente da matriz de dados usada. Os dados de RMN de 1H de NOESY, CPMG, editados por DOSY do soro ou de lipídios isolados acusaram diferenças evidentes entre os grupos analisados. Os efeitos da COVID-19 sobre metabólitos séricos foram extremos e refletem todas as respostas do corpo ao vírus SARS-CoV-2. Especialmente os altos níveis de glicose chamam a atenção e a desproporção em níveis e tipos de lipídios. Enquanto o colesterol e os esteróis livres foram muito menores em pacientes com COVID-19 grave do que em controles saudáveis, as fosfocolinas e VLDL foram as mais abundantes. Além disso, a maioria (60-75%) dos metabólitos do soro sanguíneo aumentou em 
pacientes com COVID-19 e em pacientes que foram negativos, porém com sintomas graves. Quando analisadas as vias metabólicas, muitas delas foram alteradas de maneira marcante devido à doença. Como por exemplo a biossíntese de aminoacil t-RNA, metabolismo de aminoácidos (glicina, serina e treonina), metabolismo de aminoácidos de cadeia ramificada (valina, leucina e isoleucina), metabolismo de glicerofosfolipídeos, entre outras vias. Finalmente, a RMN de 1H poderá ser usada com sucesso para classificar os pacientes graves devido múltiplas e várias alterações de metabólitos e níveis de lipídios no seu soro.",
publisher = "Associação de Usuários de RMN - AUREMN",
journal = "XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online",
title = "Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN",
url = "https://hdl.handle.net/21.15107/rcub_cer_7278"
}

Stanišić, D., Jadranin, M.,& Tasić, L.. (2020). Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN. in XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online
Associação de Usuários de RMN - AUREMN..
https://hdl.handle.net/21.15107/rcub_cer_7278

Stanišić D, Jadranin M, Tasić L. Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN. in XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online. 2020;.
https://hdl.handle.net/21.15107/rcub_cer_7278 .

Stanišić, Danijela, Jadranin, Milka, Tasić, Ljubica, "Estudo dos efeitos da COVID-19 sobre metabólitos séricos utilizando RMN" in XVI Jornada Brasileira de Ressonância Magnética, 10 a 13 de novembro de 2020, evento online (2020),
https://hdl.handle.net/21.15107/rcub_cer_7278 .

TY  - CONF
AU  - Simić, Katarina
AU  - Todorović, Nina
AU  - Miladinović, Zoran
AU  - Ivanović, Stefan
AU  - Trifunović, Snežana
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Jovanović, Vesna B.
AU  - Avramović, Nataša
AU  - Gavrilović, Aleksandra
AU  - Jovanović, Silvana
AU  - Costa, Tássia Brena Barroso Carneiro
AU  - Huan Liu, Leticia
AU  - Barros, Pedro
AU  - Stanišić, Danijela
AU  - Mandić, Boris
AU  - Tasić, Ljubica
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7536
AB  - Schizophrenia (SCZ) is a very disabling mental disorder whose molecular basis is a combination of many factors still not completely understood, with a diagnosis based on observed behavior, the person's reported experiences and reports of others that are familiar with the person, with no objective test. Also, up to date, there are no reliable markers for monitoring the SCZ. NMR-metabolomics [1] reported in 2017 bring some of the possible markers from blood serum of SCZ individuals linked strongly with known dopamine, glutamate and GABA dysfunction in SCZ. As to verify if these findings are universal, we have compared the SCZ patients from geographically different environments and cited interesting SCZ characteristics.  
The first set of samples was collected in Belgrade, Serbia. 14 mental health patients (50% male) with 52.86 ± 7.27 years of age had a confirmed diagnosis of SCZ. The control group of 13 healthy individuals (69% male) had none of psychotic disorders, and individuals were 23.07 ± 2.79 years of age. Blood serum samples were collected and prepared for the analysis following the published methodology [1, 2]. NMR spectra were measured on a Bruker AVANCE III spectrometer (500.26 MHz for 1H). The spectra were acquired at 298 K with 128 scans and 32 k. The serum samples were prepared and measured as triplicates.  
On the other side, the group of individuals from Brazil that was matched in number, age, gender and history of mental illness with individuals from Serbia was previously described [1].  
1H NMR spectra were phase and baseline corrected using MestreNova and the lactate doublet was used as the chemical shift reference. The data were binned (0.005 ppm) in a spectral range 0.50 - 9.00 ppm, while the residual HDO peak (4.50-5.00 ppm) was excluded. Then, the data were normalized by the sum equal to 1000, the variables were mean centered and PCA and PLS-DA were performed using MATLAB.  
It was shown that the mental health patients have clearly different blood serum metabolites when compared to the healthy ones independently from where the samples were obtained with almost identical marker set. Also, it was shown that the samples are different metabolically when Brazilian and Serbian samples were compared. 
1] L. Tasic et al., Schizophrenia Research 2017, 185, 182.
[2] J. Pontes et al., Analytical Methods 2017, 9, 1078.
PB  - University of Belgrade - Faculty of  Chemistry
PB  - Institute of Chemistry, Technology and Metallurgy, National Institute
C3  - Book of Abstracts - 21st Central European NMR Symposium &  Bruker Users Meeting, 21st CEUM, September 4-5, 2019, Belgrade, Serbia
T1  - Evaluation of the universality of NMR metabolic fingerprints of schizophrenia
SP  - 31
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_cer_7536
ER  - 

@conference{
author = "Simić, Katarina and Todorović, Nina and Miladinović, Zoran and Ivanović, Stefan and Trifunović, Snežana and Vujisić, Ljubodrag and Tešević, Vele and Jovanović, Vesna B. and Avramović, Nataša and Gavrilović, Aleksandra and Jovanović, Silvana and Costa, Tássia Brena Barroso Carneiro and Huan Liu, Leticia and Barros, Pedro and Stanišić, Danijela and Mandić, Boris and Tasić, Ljubica",
year = "2019",
abstract = "Schizophrenia (SCZ) is a very disabling mental disorder whose molecular basis is a combination of many factors still not completely understood, with a diagnosis based on observed behavior, the person's reported experiences and reports of others that are familiar with the person, with no objective test. Also, up to date, there are no reliable markers for monitoring the SCZ. NMR-metabolomics [1] reported in 2017 bring some of the possible markers from blood serum of SCZ individuals linked strongly with known dopamine, glutamate and GABA dysfunction in SCZ. As to verify if these findings are universal, we have compared the SCZ patients from geographically different environments and cited interesting SCZ characteristics.  
The first set of samples was collected in Belgrade, Serbia. 14 mental health patients (50% male) with 52.86 ± 7.27 years of age had a confirmed diagnosis of SCZ. The control group of 13 healthy individuals (69% male) had none of psychotic disorders, and individuals were 23.07 ± 2.79 years of age. Blood serum samples were collected and prepared for the analysis following the published methodology [1, 2]. NMR spectra were measured on a Bruker AVANCE III spectrometer (500.26 MHz for 1H). The spectra were acquired at 298 K with 128 scans and 32 k. The serum samples were prepared and measured as triplicates.  
On the other side, the group of individuals from Brazil that was matched in number, age, gender and history of mental illness with individuals from Serbia was previously described [1].  
1H NMR spectra were phase and baseline corrected using MestreNova and the lactate doublet was used as the chemical shift reference. The data were binned (0.005 ppm) in a spectral range 0.50 - 9.00 ppm, while the residual HDO peak (4.50-5.00 ppm) was excluded. Then, the data were normalized by the sum equal to 1000, the variables were mean centered and PCA and PLS-DA were performed using MATLAB.  
It was shown that the mental health patients have clearly different blood serum metabolites when compared to the healthy ones independently from where the samples were obtained with almost identical marker set. Also, it was shown that the samples are different metabolically when Brazilian and Serbian samples were compared. 
1] L. Tasic et al., Schizophrenia Research 2017, 185, 182.
[2] J. Pontes et al., Analytical Methods 2017, 9, 1078.",
publisher = "University of Belgrade - Faculty of  Chemistry, Institute of Chemistry, Technology and Metallurgy, National Institute",
journal = "Book of Abstracts - 21st Central European NMR Symposium &  Bruker Users Meeting, 21st CEUM, September 4-5, 2019, Belgrade, Serbia",
title = "Evaluation of the universality of NMR metabolic fingerprints of schizophrenia",
pages = "31-32",
url = "https://hdl.handle.net/21.15107/rcub_cer_7536"
}

Simić, K., Todorović, N., Miladinović, Z., Ivanović, S., Trifunović, S., Vujisić, L., Tešević, V., Jovanović, V. B., Avramović, N., Gavrilović, A., Jovanović, S., Costa, T. B. B. C., Huan Liu, L., Barros, P., Stanišić, D., Mandić, B.,& Tasić, L.. (2019). Evaluation of the universality of NMR metabolic fingerprints of schizophrenia. in Book of Abstracts - 21st Central European NMR Symposium &  Bruker Users Meeting, 21st CEUM, September 4-5, 2019, Belgrade, Serbia
University of Belgrade - Faculty of  Chemistry., 31-32.
https://hdl.handle.net/21.15107/rcub_cer_7536

Simić K, Todorović N, Miladinović Z, Ivanović S, Trifunović S, Vujisić L, Tešević V, Jovanović VB, Avramović N, Gavrilović A, Jovanović S, Costa TBBC, Huan Liu L, Barros P, Stanišić D, Mandić B, Tasić L. Evaluation of the universality of NMR metabolic fingerprints of schizophrenia. in Book of Abstracts - 21st Central European NMR Symposium &  Bruker Users Meeting, 21st CEUM, September 4-5, 2019, Belgrade, Serbia. 2019;:31-32.
https://hdl.handle.net/21.15107/rcub_cer_7536 .

Simić, Katarina, Todorović, Nina, Miladinović, Zoran, Ivanović, Stefan, Trifunović, Snežana, Vujisić, Ljubodrag, Tešević, Vele, Jovanović, Vesna B., Avramović, Nataša, Gavrilović, Aleksandra, Jovanović, Silvana, Costa, Tássia Brena Barroso Carneiro, Huan Liu, Leticia, Barros, Pedro, Stanišić, Danijela, Mandić, Boris, Tasić, Ljubica, "Evaluation of the universality of NMR metabolic fingerprints of schizophrenia" in Book of Abstracts - 21st Central European NMR Symposium &  Bruker Users Meeting, 21st CEUM, September 4-5, 2019, Belgrade, Serbia (2019):31-32,
https://hdl.handle.net/21.15107/rcub_cer_7536 .