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The DsbA signal peptide-mediated secretion of a highly efficient raw-starch-digesting, recombinant alpha-amylase from Bacillus licheniformis ATCC 9945a
dc.creator | Božić, Nataša | |
dc.creator | Puertas, Juan-Miguel | |
dc.creator | Lončar, Nikola | |
dc.creator | Sans, Duran Cristina | |
dc.creator | Lopez-Santin, Josep | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2019-01-30T17:38:12Z | |
dc.date.available | 2019-01-30T17:38:12Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1359-5113 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/1368 | |
dc.description.abstract | In this study, a new approach for extracellular production of recombinant alpha-amylase in Escherichia coli was investigated. A gene encoding a highly efficient raw-starch-digesting alpha-amylase from Bacillus licheniformis ATCC 9945a was cloned and expressed in E. coli. The gene encoding mature alpha-amylase was cloned into the pDAss expression vector, and secretion of the gene product was regulated by fusion to the signal peptide of DsbA, a well-characterized E. coli periplasmic protein. E. coli BL21 (DE3) carrying pDAss vector containing amylase gene had approximately 2.5-fold higher volumetric enzyme productivity than the natural system. The recombinant enzyme showed higher efficiency for digesting diverse raw starches when compared with the native enzyme and was similar to commercial alpha-amylase in its ability to hydrolyze raw starches. The properties of the recombinant enzyme demonstrate the potential of the DsbA signal peptide approach for the secretory production of the fully active, industrially important recombinant enzyme. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172048/RS// | |
dc.relation | Federation of European Biochemical Societies | |
dc.relation | Joint Serbian-Spanish Action | |
dc.relation | Spanish MICINN [CTQ2011-28398-CO2-01] | |
dc.rights | restrictedAccess | |
dc.source | Process Biochemistry | |
dc.subject | alpha-Amylase | en |
dc.subject | Bacillus licheniformis | en |
dc.subject | Escherichia coli | en |
dc.subject | DsbA signal peptide | en |
dc.subject | Raw starch hydrolysis | en |
dc.title | The DsbA signal peptide-mediated secretion of a highly efficient raw-starch-digesting, recombinant alpha-amylase from Bacillus licheniformis ATCC 9945a | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Божић, Наташа; Вујциц, Зоран; Лонцар, Никола; Лопез-Сантин, Јосеп; Пуертас, Јуан-Мигуел; Санс, Дуран Цристина; | |
dc.citation.volume | 48 | |
dc.citation.issue | 3 | |
dc.citation.spage | 438 | |
dc.citation.epage | 442 | |
dc.citation.other | 48(3): 438-442 | |
dc.citation.rank | M21 | |
dc.identifier.doi | 10.1016/j.procbio.2013.01.016 | |
dc.identifier.scopus | 2-s2.0-84875906938 | |
dc.identifier.wos | 000318262900008 | |
dc.type.version | publishedVersion |