Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis
Само за регистроване кориснике
2017
Аутори
Sentić, MilicaSojic, Neso
Paolucci, Francesco
Arbault, Stéphane
Rapino, Stefania
Villani, Elena
Jović, Milica
Lesch, Andreas
Goudeau, Bertrand
Scarabino, Sabina
Giovanni, Valenti
Чланак у часопису (Објављена верзија)
,
ACS
Метаподаци
Приказ свих података о документуАпстракт
We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in the immediate vicinity of the electrode surface (i.e., 1–2 μm). Then, to present the potential diagnostic applications of our approach, we selected carbon nanotubes (CNT)-based inkjet-printed disposable electrodes for the direct ECL imaging of a labeled plasma receptor overexpressed on tumor cells. The ECL fluorophore was linked to an antibody and enabled to localize the ECL generation on the cancer cell membrane in close proximity to the electrode surface. Such a result is intrinsically associated with the unique ECL mechanism and is rationalized by considering the limited lifetimes of the electrogenerated coreactant radicals. The electrochemical stimulus use...d for luminescence generation does not suffer from background signals, such as the typical autofluorescence of biological samples. The presented surface-confined ECL microscopy should find promising applications in ultrasensitive single cell imaging assays.
Кључне речи:
Carbon nanotubes / Cells / Electrochemical cells / Electrodes / Peptides and proteinsИзвор:
Journal of the American Chemical Society, 2017, 139, 46, 16830-16837Издавач:
- American Chemical Society (ACS)
DOI: 10.1021/jacs.7b09260
ISSN: 0002-7863
PubMed: 29064235
WoS: 000416496400056
Scopus: 2-s2.0-85034826312
Институција/група
IHTMTY - JOUR AU - Sentić, Milica AU - Sojic, Neso AU - Paolucci, Francesco AU - Arbault, Stéphane AU - Rapino, Stefania AU - Villani, Elena AU - Jović, Milica AU - Lesch, Andreas AU - Goudeau, Bertrand AU - Scarabino, Sabina AU - Giovanni, Valenti PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/7132 AB - We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in the immediate vicinity of the electrode surface (i.e., 1–2 μm). Then, to present the potential diagnostic applications of our approach, we selected carbon nanotubes (CNT)-based inkjet-printed disposable electrodes for the direct ECL imaging of a labeled plasma receptor overexpressed on tumor cells. The ECL fluorophore was linked to an antibody and enabled to localize the ECL generation on the cancer cell membrane in close proximity to the electrode surface. Such a result is intrinsically associated with the unique ECL mechanism and is rationalized by considering the limited lifetimes of the electrogenerated coreactant radicals. The electrochemical stimulus used for luminescence generation does not suffer from background signals, such as the typical autofluorescence of biological samples. The presented surface-confined ECL microscopy should find promising applications in ultrasensitive single cell imaging assays. PB - American Chemical Society (ACS) T2 - Journal of the American Chemical Society T1 - Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis VL - 139 IS - 46 SP - 16830 EP - 16837 DO - 10.1021/jacs.7b09260 ER -
@article{ author = "Sentić, Milica and Sojic, Neso and Paolucci, Francesco and Arbault, Stéphane and Rapino, Stefania and Villani, Elena and Jović, Milica and Lesch, Andreas and Goudeau, Bertrand and Scarabino, Sabina and Giovanni, Valenti", year = "2017", abstract = "We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in the immediate vicinity of the electrode surface (i.e., 1–2 μm). Then, to present the potential diagnostic applications of our approach, we selected carbon nanotubes (CNT)-based inkjet-printed disposable electrodes for the direct ECL imaging of a labeled plasma receptor overexpressed on tumor cells. The ECL fluorophore was linked to an antibody and enabled to localize the ECL generation on the cancer cell membrane in close proximity to the electrode surface. Such a result is intrinsically associated with the unique ECL mechanism and is rationalized by considering the limited lifetimes of the electrogenerated coreactant radicals. The electrochemical stimulus used for luminescence generation does not suffer from background signals, such as the typical autofluorescence of biological samples. The presented surface-confined ECL microscopy should find promising applications in ultrasensitive single cell imaging assays.", publisher = "American Chemical Society (ACS)", journal = "Journal of the American Chemical Society", title = "Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis", volume = "139", number = "46", pages = "16830-16837", doi = "10.1021/jacs.7b09260" }
Sentić, M., Sojic, N., Paolucci, F., Arbault, S., Rapino, S., Villani, E., Jović, M., Lesch, A., Goudeau, B., Scarabino, S.,& Giovanni, V.. (2017). Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis. in Journal of the American Chemical Society American Chemical Society (ACS)., 139(46), 16830-16837. https://doi.org/10.1021/jacs.7b09260
Sentić M, Sojic N, Paolucci F, Arbault S, Rapino S, Villani E, Jović M, Lesch A, Goudeau B, Scarabino S, Giovanni V. Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis. in Journal of the American Chemical Society. 2017;139(46):16830-16837. doi:10.1021/jacs.7b09260 .
Sentić, Milica, Sojic, Neso, Paolucci, Francesco, Arbault, Stéphane, Rapino, Stefania, Villani, Elena, Jović, Milica, Lesch, Andreas, Goudeau, Bertrand, Scarabino, Sabina, Giovanni, Valenti, "Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis" in Journal of the American Chemical Society, 139, no. 46 (2017):16830-16837, https://doi.org/10.1021/jacs.7b09260 . .