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Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases
dc.creator | Radosavljević, Jelena | |
dc.creator | Dobrijevic, Dragana | |
dc.creator | Jadranin, Milka | |
dc.creator | Blanusa, Milan | |
dc.creator | Vukmirica, Jelena | |
dc.creator | Ćirković Veličković, Tanja | |
dc.date.accessioned | 2019-01-30T17:24:04Z | |
dc.date.available | 2019-01-30T17:24:04Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0022-5142 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/709 | |
dc.description.abstract | BACKGROUND: The major peanut allergens are Ara h 1, Ara h 2 and Ara h 6. Proteolytic processing has been shown to be required for the maturation process of Ara h 6. The aim of this study was to examine whether Ara h 2 undergoes proteolytic processing and, if so, whether proteolytic processing influences its ability to bind human immunoglobulin E (IgE). RESULTS: Ara h 2 isolated from peanut extract under conditions of protease inhibition revealed a single additional peak for its two known isoforms (Ara h 2.01 and Ara h 2.02), corresponding to a C-terminally truncated form lacking a dipeptide (RY). Ara h 2 isolated in the absence of protease inhibition, however, yielded two additional peaks, identified as C-terminally truncated forms lacking either a dipeptide (RY) or a single tyrosine residue. The IgE-binding capacity of the Ara h 2 truncated forms was not altered. CONCLUSION: Ara h 2 undergoes proteolytic processing by peanut proteases that involves C-terminal removal of a dipeptide. Hence Ara h 2 isolated from peanut extract is a complex mixture of two isoforms expressed by different genes, Ara h 2.01 and Ara h 2.02, as well as truncated forms generated by the proteolytic processing of these isoforms. | en |
dc.publisher | John Wiley & Sons Ltd, Chichester | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of the Science of Food and Agriculture | |
dc.subject | Ara h 2 | en |
dc.subject | Ara h 6 | en |
dc.subject | proteolysis | en |
dc.subject | IgE binding | en |
dc.subject | food allergy | en |
dc.title | Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Јадранин, Милка; Блануса, Милан; Добријевиц, Драгана; Велицковиц, Тања Цирковиц; Вукмирица, Јелена; Радосављевиц, Јелена; | |
dc.citation.volume | 90 | |
dc.citation.issue | 10 | |
dc.citation.spage | 1702 | |
dc.citation.epage | 1708 | |
dc.citation.other | 90(10): 1702-1708 | |
dc.citation.rank | M21 | |
dc.identifier.pmid | 20564442 | |
dc.identifier.doi | 10.1002/jsfa.4005 | |
dc.identifier.scopus | 2-s2.0-77954404613 | |
dc.identifier.wos | 000280121600018 | |
dc.type.version | publishedVersion |