Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases
Само за регистроване кориснике
2010
Аутори
Radosavljević, JelenaDobrijevic, Dragana
Jadranin, Milka
Blanusa, Milan
Vukmirica, Jelena
Ćirković Veličković, Tanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BACKGROUND: The major peanut allergens are Ara h 1, Ara h 2 and Ara h 6. Proteolytic processing has been shown to be required for the maturation process of Ara h 6. The aim of this study was to examine whether Ara h 2 undergoes proteolytic processing and, if so, whether proteolytic processing influences its ability to bind human immunoglobulin E (IgE). RESULTS: Ara h 2 isolated from peanut extract under conditions of protease inhibition revealed a single additional peak for its two known isoforms (Ara h 2.01 and Ara h 2.02), corresponding to a C-terminally truncated form lacking a dipeptide (RY). Ara h 2 isolated in the absence of protease inhibition, however, yielded two additional peaks, identified as C-terminally truncated forms lacking either a dipeptide (RY) or a single tyrosine residue. The IgE-binding capacity of the Ara h 2 truncated forms was not altered. CONCLUSION: Ara h 2 undergoes proteolytic processing by peanut proteases that involves C-terminal removal of a dipeptide. H...ence Ara h 2 isolated from peanut extract is a complex mixture of two isoforms expressed by different genes, Ara h 2.01 and Ara h 2.02, as well as truncated forms generated by the proteolytic processing of these isoforms.
Кључне речи:
Ara h 2 / Ara h 6 / proteolysis / IgE binding / food allergyИзвор:
Journal of the Science of Food and Agriculture, 2010, 90, 10, 1702-1708Издавач:
- John Wiley & Sons Ltd, Chichester
Финансирање / пројекти:
- Испитивање структуре и функције биолошки важних макромолекула у физиолошким и патолошким стањима (RS-142020)
DOI: 10.1002/jsfa.4005
ISSN: 0022-5142
PubMed: 20564442
WoS: 000280121600018
Scopus: 2-s2.0-77954404613
Институција/група
IHTMTY - JOUR AU - Radosavljević, Jelena AU - Dobrijevic, Dragana AU - Jadranin, Milka AU - Blanusa, Milan AU - Vukmirica, Jelena AU - Ćirković Veličković, Tanja PY - 2010 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/709 AB - BACKGROUND: The major peanut allergens are Ara h 1, Ara h 2 and Ara h 6. Proteolytic processing has been shown to be required for the maturation process of Ara h 6. The aim of this study was to examine whether Ara h 2 undergoes proteolytic processing and, if so, whether proteolytic processing influences its ability to bind human immunoglobulin E (IgE). RESULTS: Ara h 2 isolated from peanut extract under conditions of protease inhibition revealed a single additional peak for its two known isoforms (Ara h 2.01 and Ara h 2.02), corresponding to a C-terminally truncated form lacking a dipeptide (RY). Ara h 2 isolated in the absence of protease inhibition, however, yielded two additional peaks, identified as C-terminally truncated forms lacking either a dipeptide (RY) or a single tyrosine residue. The IgE-binding capacity of the Ara h 2 truncated forms was not altered. CONCLUSION: Ara h 2 undergoes proteolytic processing by peanut proteases that involves C-terminal removal of a dipeptide. Hence Ara h 2 isolated from peanut extract is a complex mixture of two isoforms expressed by different genes, Ara h 2.01 and Ara h 2.02, as well as truncated forms generated by the proteolytic processing of these isoforms. PB - John Wiley & Sons Ltd, Chichester T2 - Journal of the Science of Food and Agriculture T1 - Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases VL - 90 IS - 10 SP - 1702 EP - 1708 DO - 10.1002/jsfa.4005 ER -
@article{ author = "Radosavljević, Jelena and Dobrijevic, Dragana and Jadranin, Milka and Blanusa, Milan and Vukmirica, Jelena and Ćirković Veličković, Tanja", year = "2010", abstract = "BACKGROUND: The major peanut allergens are Ara h 1, Ara h 2 and Ara h 6. Proteolytic processing has been shown to be required for the maturation process of Ara h 6. The aim of this study was to examine whether Ara h 2 undergoes proteolytic processing and, if so, whether proteolytic processing influences its ability to bind human immunoglobulin E (IgE). RESULTS: Ara h 2 isolated from peanut extract under conditions of protease inhibition revealed a single additional peak for its two known isoforms (Ara h 2.01 and Ara h 2.02), corresponding to a C-terminally truncated form lacking a dipeptide (RY). Ara h 2 isolated in the absence of protease inhibition, however, yielded two additional peaks, identified as C-terminally truncated forms lacking either a dipeptide (RY) or a single tyrosine residue. The IgE-binding capacity of the Ara h 2 truncated forms was not altered. CONCLUSION: Ara h 2 undergoes proteolytic processing by peanut proteases that involves C-terminal removal of a dipeptide. Hence Ara h 2 isolated from peanut extract is a complex mixture of two isoforms expressed by different genes, Ara h 2.01 and Ara h 2.02, as well as truncated forms generated by the proteolytic processing of these isoforms.", publisher = "John Wiley & Sons Ltd, Chichester", journal = "Journal of the Science of Food and Agriculture", title = "Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases", volume = "90", number = "10", pages = "1702-1708", doi = "10.1002/jsfa.4005" }
Radosavljević, J., Dobrijevic, D., Jadranin, M., Blanusa, M., Vukmirica, J.,& Ćirković Veličković, T.. (2010). Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases. in Journal of the Science of Food and Agriculture John Wiley & Sons Ltd, Chichester., 90(10), 1702-1708. https://doi.org/10.1002/jsfa.4005
Radosavljević J, Dobrijevic D, Jadranin M, Blanusa M, Vukmirica J, Ćirković Veličković T. Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases. in Journal of the Science of Food and Agriculture. 2010;90(10):1702-1708. doi:10.1002/jsfa.4005 .
Radosavljević, Jelena, Dobrijevic, Dragana, Jadranin, Milka, Blanusa, Milan, Vukmirica, Jelena, Ćirković Veličković, Tanja, "Insights into proteolytic processing of the major peanut allergen Ara h 2 by endogenous peanut proteases" in Journal of the Science of Food and Agriculture, 90, no. 10 (2010):1702-1708, https://doi.org/10.1002/jsfa.4005 . .