Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O
Само за регистроване кориснике
2020
Аутори
Srajer-Gajdošik, MartinaAnđelković, Uroš
Gašo Sokač, Dajana
Pavlović, Hrvoje
Shevchuk, Olga
Martinović, Tamara
Clifton, James
Begić, Marija
Josić, Djuro
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down-regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed.
Кључне речи:
Foodborne pathogens / Listeriolysin O / Mechanism of action / Proteome changes / Growth inhibition / Biomarker candidate / Fast diagnosis of listeriosisИзвор:
Food Research International, 2020, 131, 108951-Издавач:
- Elsevier
DOI: 10.1016/j.foodres.2019.108951
ISSN: 0963-9969
PubMed: 32247447
WoS: 000528252800060
Scopus: 2-s2.0-85077748608
Институција/група
IHTMTY - JOUR AU - Srajer-Gajdošik, Martina AU - Anđelković, Uroš AU - Gašo Sokač, Dajana AU - Pavlović, Hrvoje AU - Shevchuk, Olga AU - Martinović, Tamara AU - Clifton, James AU - Begić, Marija AU - Josić, Djuro PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/6923 AB - Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down-regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed. PB - Elsevier T2 - Food Research International T1 - Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O VL - 131 SP - 108951 DO - 10.1016/j.foodres.2019.108951 ER -
@article{ author = "Srajer-Gajdošik, Martina and Anđelković, Uroš and Gašo Sokač, Dajana and Pavlović, Hrvoje and Shevchuk, Olga and Martinović, Tamara and Clifton, James and Begić, Marija and Josić, Djuro", year = "2020", abstract = "Proteomic analysis of foodborne pathogen Listeria monocytogenes after treatment with three disinfectants based on ammonium salts of pyridoxal oxime (POD) reveal perturbation of cellular processes. These inhibitors caused disturbance in the synthesis of plasma membrane proteins and cell wall proteoglycans. Some of key proteins and proteoglycans from these two groups that are important for bacterial growth are down-regulated. Additionally, we demonstrated that the main bacterial toxin Listeriolysin O (LLO) is significantly down-regulated after treatment with each of three investigated inhibitors. These investigations confirm already postulated mechanism of action of POD-based inhibitors that results in disturbance of key cell surface proteins and proteoglycans in Gram-positive bacteria. Additionally, the use of some proteins such as LLO, as potential biomarker candidates of food poisoning with this bacterium is discussed.", publisher = "Elsevier", journal = "Food Research International", title = "Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O", volume = "131", pages = "108951", doi = "10.1016/j.foodres.2019.108951" }
Srajer-Gajdošik, M., Anđelković, U., Gašo Sokač, D., Pavlović, H., Shevchuk, O., Martinović, T., Clifton, J., Begić, M.,& Josić, D.. (2020). Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O. in Food Research International Elsevier., 131, 108951. https://doi.org/10.1016/j.foodres.2019.108951
Srajer-Gajdošik M, Anđelković U, Gašo Sokač D, Pavlović H, Shevchuk O, Martinović T, Clifton J, Begić M, Josić D. Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O. in Food Research International. 2020;131:108951. doi:10.1016/j.foodres.2019.108951 .
Srajer-Gajdošik, Martina, Anđelković, Uroš, Gašo Sokač, Dajana, Pavlović, Hrvoje, Shevchuk, Olga, Martinović, Tamara, Clifton, James, Begić, Marija, Josić, Djuro, "Proteomic analysis of pyridoxal oxime derivatives treated Listeria monocytogenes reveals down-regulation of the main virulence factor, Listeriolysin O" in Food Research International, 131 (2020):108951, https://doi.org/10.1016/j.foodres.2019.108951 . .