Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
Samo za registrovane korisnike
2009
Autori
Spasojević, IvanMojović, Miloš
Blagojević, Duško
Spasić, Snežana
Jones, David
Nikolić-Kokić, Aleksandra
Spasić, Mihajlo
Članak u časopisu (Objavljena verzija)
,
Elsevier
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16...BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
Ključne reči:
Fructose / Glucose / Hydroxyl radical / Mannitol / EPR spectroscopy / Haber–Weiss reactionIzvor:
Carbohydrate Research, 2009, 344, 1, 80-84Izdavač:
- Elsevier
Finansiranje / projekti:
- Uloga redoks aktivnih supstanci u procesima održavanja homeostaze živih sistema (RS-143034)
- Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-143016)
DOI: 10.1016/j.carres.2008.09.025
ISSN: 0008-6215; 1873-426X
WoS: 000262596200011
Scopus: 2-s2.0-57749111966
Institucija/grupa
IHTMTY - JOUR AU - Spasojević, Ivan AU - Mojović, Miloš AU - Blagojević, Duško AU - Spasić, Snežana AU - Jones, David AU - Nikolić-Kokić, Aleksandra AU - Spasić, Mihajlo PY - 2009 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3937 AB - The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy. PB - Elsevier T2 - Carbohydrate Research T1 - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical VL - 344 IS - 1 SP - 80 EP - 84 DO - 10.1016/j.carres.2008.09.025 ER -
@article{ author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasić, Snežana and Jones, David and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo", year = "2009", abstract = "The hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.", publisher = "Elsevier", journal = "Carbohydrate Research", title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical", volume = "344", number = "1", pages = "80-84", doi = "10.1016/j.carres.2008.09.025" }
Spasojević, I., Mojović, M., Blagojević, D., Spasić, S., Jones, D., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research Elsevier., 344(1), 80-84. https://doi.org/10.1016/j.carres.2008.09.025
Spasojević I, Mojović M, Blagojević D, Spasić S, Jones D, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84. doi:10.1016/j.carres.2008.09.025 .
Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasić, Snežana, Jones, David, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84, https://doi.org/10.1016/j.carres.2008.09.025 . .