SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
Samo za registrovane korisnike
2020
Autori
Nešić, Maja D.Dučić, Tanja
Liang, Xinyue
Algarra, Manuel
Mi, Lan
Korićanac, Lela
Žakula, Jelena
Kop, Tatjana
Bjelaković, Mira
Mitrović, Aleksandra
Gojgić-Cvijović, Gordana
Stepić, Milutin
Petković, Marijana
Članak u časopisu (Objavljena verzija)
,
Elsevier
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Abstract
Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but... these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
Ključne reči:
Pullulan-C60 / Levan-C60 / Synchrotron radiation FTIR spectro-microscopyIzvor:
International Journal of Biological Macromolecules, 2020, 165, B, 2541-2549Izdavač:
- Elsevier
Finansiranje / projekti:
- Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação (M1420-01-0145-FEDER-000005)
- Ministry of Science and Technology of the People's Republic of China (SINO-SERBIA2018002)
- Madeira 14-20 Program (RAM-M1420-01-0145-FEDER-000008)
- Serbian-Chinese bilateral project (451-00-478/2018-09/16)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-MESTD-inst-2020-200026)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200017 (Univerzitet u Beogradu, Institut za nuklearne nauke Vinča, Beograd-Vinča) (RS-MESTD-inst-2020-200017)
- ALBA Synchrotron (Grant No. 2019093770)
- Fundação para a Ciência e a Tecnologia (PEstOE/QUI/UI0674/2019)
- Fundação para a Ciência e a Tecnologia (INNOINDIGO/0001/2015)
- Fundação para a Ciência e a Tecnologia (UID/MAT/00006/2019)
- Ministerio de Ciencia, Innovación y Universidades (RTI2018-099668-BC22)
- Junta de Andalucía and FEDER funds (UMA18-FEDERJA-126)
DOI: 10.1016/j.ijbiomac.2020.10.141
ISSN: 0141-8130; 1879-0003 (web)
WoS: 000600773500088
Scopus: 2-s2.0-85094207129
Institucija/grupa
IHTMTY - JOUR AU - Nešić, Maja D. AU - Dučić, Tanja AU - Liang, Xinyue AU - Algarra, Manuel AU - Mi, Lan AU - Korićanac, Lela AU - Žakula, Jelena AU - Kop, Tatjana AU - Bjelaković, Mira AU - Mitrović, Aleksandra AU - Gojgić-Cvijović, Gordana AU - Stepić, Milutin AU - Petković, Marijana PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3891 AB - Abstract Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells. PB - Elsevier T2 - International Journal of Biological Macromolecules T1 - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives VL - 165 IS - B SP - 2541 EP - 2549 DO - 10.1016/j.ijbiomac.2020.10.141 ER -
@article{ author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana and Bjelaković, Mira and Mitrović, Aleksandra and Gojgić-Cvijović, Gordana and Stepić, Milutin and Petković, Marijana", year = "2020", abstract = "Abstract Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.", publisher = "Elsevier", journal = "International Journal of Biological Macromolecules", title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives", volume = "165", number = "B", pages = "2541-2549", doi = "10.1016/j.ijbiomac.2020.10.141" }
Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T., Bjelaković, M., Mitrović, A., Gojgić-Cvijović, G., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules Elsevier., 165(B), 2541-2549. https://doi.org/10.1016/j.ijbiomac.2020.10.141
Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop T, Bjelaković M, Mitrović A, Gojgić-Cvijović G, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165(B):2541-2549. doi:10.1016/j.ijbiomac.2020.10.141 .
Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana, Bjelaković, Mira, Mitrović, Aleksandra, Gojgić-Cvijović, Gordana, Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165, no. B (2020):2541-2549, https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .