Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone
Hemijske modifikacije lektina morskog korala Gerardia savaglia morskim hinonom avaronom
2007
Аутори
Pajic, IvanaVujčić, Zoran
Vujčić, Miroslava
Novaković, Irena
Sladić, Dušan
Gašić, Miroslav J.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes.
Avaron, hinon izolovan iz morskog sunđera Dysidea avara, poseduje sposobnost da hemijski modifikuje proteine. U ovom radu ispitivana je modifikacija lektina izolovanog iz korala Gerardia savaglia avaronom. Tehnike za praćenje hemijske modifikacije bile su: SDS PAGE, izoelektrično fokusiranje i hemaglutinacioni test. Rezultati SDS PAGE upućuju na dimerizaciju proteina. Dolazi do pomeranja pI vrednosti proteina. Promena hemaglutinacione aktivnosti G. savaglia lektina avaronom uticala je na njegovu sposobnost interakcije sa membranom eritrocita.
Кључне речи:
avarone / quinone / Gerardia savaglia lectin / covalent modificationИзвор:
Journal of the Serbian Chemical Society, 2007, 72, 12, 1271-1274Издавач:
- Serbian Chemical Soc, Belgrade
Финансирање / пројекти:
- Интеракције природних производа и њихових аналога са протеинима и нуклеинским киселинама (RS-MESTD-MPN2006-2010-142026)
DOI: 10.2298/JSC0712271P
ISSN: 0352-5139
WoS: 000252412100011
Scopus: 2-s2.0-36949025210
Институција/група
IHTMTY - JOUR AU - Pajic, Ivana AU - Vujčić, Zoran AU - Vujčić, Miroslava AU - Novaković, Irena AU - Sladić, Dušan AU - Gašić, Miroslav J. PY - 2007 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/330 AB - The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes. AB - Avaron, hinon izolovan iz morskog sunđera Dysidea avara, poseduje sposobnost da hemijski modifikuje proteine. U ovom radu ispitivana je modifikacija lektina izolovanog iz korala Gerardia savaglia avaronom. Tehnike za praćenje hemijske modifikacije bile su: SDS PAGE, izoelektrično fokusiranje i hemaglutinacioni test. Rezultati SDS PAGE upućuju na dimerizaciju proteina. Dolazi do pomeranja pI vrednosti proteina. Promena hemaglutinacione aktivnosti G. savaglia lektina avaronom uticala je na njegovu sposobnost interakcije sa membranom eritrocita. PB - Serbian Chemical Soc, Belgrade T2 - Journal of the Serbian Chemical Society T1 - Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone T1 - Hemijske modifikacije lektina morskog korala Gerardia savaglia morskim hinonom avaronom VL - 72 IS - 12 SP - 1271 EP - 1274 DO - 10.2298/JSC0712271P ER -
@article{ author = "Pajic, Ivana and Vujčić, Zoran and Vujčić, Miroslava and Novaković, Irena and Sladić, Dušan and Gašić, Miroslav J.", year = "2007", abstract = "The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes., Avaron, hinon izolovan iz morskog sunđera Dysidea avara, poseduje sposobnost da hemijski modifikuje proteine. U ovom radu ispitivana je modifikacija lektina izolovanog iz korala Gerardia savaglia avaronom. Tehnike za praćenje hemijske modifikacije bile su: SDS PAGE, izoelektrično fokusiranje i hemaglutinacioni test. Rezultati SDS PAGE upućuju na dimerizaciju proteina. Dolazi do pomeranja pI vrednosti proteina. Promena hemaglutinacione aktivnosti G. savaglia lektina avaronom uticala je na njegovu sposobnost interakcije sa membranom eritrocita.", publisher = "Serbian Chemical Soc, Belgrade", journal = "Journal of the Serbian Chemical Society", title = "Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone, Hemijske modifikacije lektina morskog korala Gerardia savaglia morskim hinonom avaronom", volume = "72", number = "12", pages = "1271-1274", doi = "10.2298/JSC0712271P" }
Pajic, I., Vujčić, Z., Vujčić, M., Novaković, I., Sladić, D.,& Gašić, M. J.. (2007). Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone. in Journal of the Serbian Chemical Society Serbian Chemical Soc, Belgrade., 72(12), 1271-1274. https://doi.org/10.2298/JSC0712271P
Pajic I, Vujčić Z, Vujčić M, Novaković I, Sladić D, Gašić MJ. Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone. in Journal of the Serbian Chemical Society. 2007;72(12):1271-1274. doi:10.2298/JSC0712271P .
Pajic, Ivana, Vujčić, Zoran, Vujčić, Miroslava, Novaković, Irena, Sladić, Dušan, Gašić, Miroslav J., "Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone" in Journal of the Serbian Chemical Society, 72, no. 12 (2007):1271-1274, https://doi.org/10.2298/JSC0712271P . .