Electrochemical behavior of immobilized hemoglobin in alkaline solution
Само за регистроване кориснике
2017
Аутори
Jović-Jovičić, NatašaMojović, Zorica
Mojović, Miloš
Banković, Predrag
Ajduković, Marija
Milutinović Nikolić, Aleksandra
Jovanović, Dušan M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H2O2. The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sul-fate (SDS) loadings. The obtained materials were characterized using DR UV vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorpora-tion in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at -0.45 V and -0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) - highly reduced form of hemoglobin respectively. The latter pea...k reflects hemoglobin denaturation. The presence of H2O2 in the alkaline solution increased current intensities corresponding to both peaks (-0.45 V and -0.7 V). Linear response of peak current intensity vs. H2O2 concentration was monitored for all investigated samples within different H2O2 concentration ranges. The AA-SDSI.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(mu A)= 7.99 +1.056 x1f1202] (mM) (R=0.996). The limit of detection of 28 mu M was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained val-ues of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation.
Кључне речи:
Acid activated clay / Alkaline electrolyte / Hemoglobin / Modified electrode / Sodium dodecyl sulfateИзвор:
Applied Surface Science, 2017, 400, 347-354Издавач:
- Elsevier
Финансирање / пројекти:
- Наноструктурни функционални и композитни материјали у каталитичким и сорпционим процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45001)
- Биомаркери у неуродегенеративним и малигним процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
DOI: 10.1016/j.apsusc.2016.12.151
ISSN: 0169-4332
WoS: 000394201800043
Scopus: 2-s2.0-85007609871
Институција/група
IHTMTY - JOUR AU - Jović-Jovičić, Nataša AU - Mojović, Zorica AU - Mojović, Miloš AU - Banković, Predrag AU - Ajduković, Marija AU - Milutinović Nikolić, Aleksandra AU - Jovanović, Dušan M. PY - 2017 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/2196 AB - Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H2O2. The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sul-fate (SDS) loadings. The obtained materials were characterized using DR UV vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorpora-tion in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at -0.45 V and -0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) - highly reduced form of hemoglobin respectively. The latter peak reflects hemoglobin denaturation. The presence of H2O2 in the alkaline solution increased current intensities corresponding to both peaks (-0.45 V and -0.7 V). Linear response of peak current intensity vs. H2O2 concentration was monitored for all investigated samples within different H2O2 concentration ranges. The AA-SDSI.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(mu A)= 7.99 +1.056 x1f1202] (mM) (R=0.996). The limit of detection of 28 mu M was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained val-ues of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation. PB - Elsevier T2 - Applied Surface Science T1 - Electrochemical behavior of immobilized hemoglobin in alkaline solution VL - 400 SP - 347 EP - 354 DO - 10.1016/j.apsusc.2016.12.151 ER -
@article{ author = "Jović-Jovičić, Nataša and Mojović, Zorica and Mojović, Miloš and Banković, Predrag and Ajduković, Marija and Milutinović Nikolić, Aleksandra and Jovanović, Dušan M.", year = "2017", abstract = "Glassy carbon electrode was modified with different synthesized hybrid clay-based materials and tested in alkaline solution with and without H2O2. The hybrid materials were obtained by immobilizing hemoglobin (Hb) on acid activated (AA) clay, or on AA clay modified with different sodium dodecyl sul-fate (SDS) loadings. The obtained materials were characterized using DR UV vis and ESR spectroscopy, elemental analysis, and SEM. The characterization confirmed higher degree of hemoglobin incorpora-tion in the presence of SDS. The presence of SDS on the surface of clay particles resulted in the partial oxidation/denaturation of hemoglobin and formation of hemichrome. Cyclic voltammetry was used for the investigation of the electrochemical behavior of immobilized hemoglobin in alkaline solution. Two cathodic peaks at -0.45 V and -0.70 V were recorded and ascribed to the reduction of heme Fe(III)/Fe(II), and formation of HbFe(I) - highly reduced form of hemoglobin respectively. The latter peak reflects hemoglobin denaturation. The presence of H2O2 in the alkaline solution increased current intensities corresponding to both peaks (-0.45 V and -0.7 V). Linear response of peak current intensity vs. H2O2 concentration was monitored for all investigated samples within different H2O2 concentration ranges. The AA-SDSI.0-Hb electrode exhibited the highest current response with linear regression equation in the following form: I(mu A)= 7.99 +1.056 x1f1202] (mM) (R=0.996). The limit of detection of 28 mu M was estimated using the 3 sigma method. Different modified electrodes exhibited different degrees of denaturation resistance. The obtained val-ues of Michaelis-Menten constant indicated that prolonged cycling in the presence of SDS increases protein denaturation.", publisher = "Elsevier", journal = "Applied Surface Science", title = "Electrochemical behavior of immobilized hemoglobin in alkaline solution", volume = "400", pages = "347-354", doi = "10.1016/j.apsusc.2016.12.151" }
Jović-Jovičić, N., Mojović, Z., Mojović, M., Banković, P., Ajduković, M., Milutinović Nikolić, A.,& Jovanović, D. M.. (2017). Electrochemical behavior of immobilized hemoglobin in alkaline solution. in Applied Surface Science Elsevier., 400, 347-354. https://doi.org/10.1016/j.apsusc.2016.12.151
Jović-Jovičić N, Mojović Z, Mojović M, Banković P, Ajduković M, Milutinović Nikolić A, Jovanović DM. Electrochemical behavior of immobilized hemoglobin in alkaline solution. in Applied Surface Science. 2017;400:347-354. doi:10.1016/j.apsusc.2016.12.151 .
Jović-Jovičić, Nataša, Mojović, Zorica, Mojović, Miloš, Banković, Predrag, Ajduković, Marija, Milutinović Nikolić, Aleksandra, Jovanović, Dušan M., "Electrochemical behavior of immobilized hemoglobin in alkaline solution" in Applied Surface Science, 400 (2017):347-354, https://doi.org/10.1016/j.apsusc.2016.12.151 . .