Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase
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2016
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Prikaz svih podataka o dokumentuApstrakt
alpha-Amylase from Bacillus licheniformis ATCC 9945a (BliAmy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of BliAmy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), BliAmy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. BliAmy presents interesting performances on highly concentrated solid starch and could be of value for starch-consumi...ng industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis.
Ključne reči:
Bacillus sp. / Amylase / Fed-batch / Purification / Statistical experimental design / Raw starch degradationIzvor:
Applied Microbiology and Biotechnology, 2016, 100, 6, 2709-2719Izdavač:
- Springer, New York
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-MESTD-Basic Research (BR or ON)-172048)
- International Centre for Genetic Engineering and Biotechnology (ICGEB) [CRP/YUG11-02]
DOI: 10.1007/s00253-015-7101-4
ISSN: 0175-7598
WoS: 000371244300018
Scopus: 2-s2.0-84959107103
Institucija/grupa
IHTMTY - JOUR AU - Šokarda Slavić, Marinela AU - Pešić, Milja AU - Vujčić, Zoran AU - Božić, Nataša PY - 2016 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1965 AB - alpha-Amylase from Bacillus licheniformis ATCC 9945a (BliAmy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of BliAmy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), BliAmy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. BliAmy presents interesting performances on highly concentrated solid starch and could be of value for starch-consuming industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase VL - 100 IS - 6 SP - 2709 EP - 2719 DO - 10.1007/s00253-015-7101-4 ER -
@article{ author = "Šokarda Slavić, Marinela and Pešić, Milja and Vujčić, Zoran and Božić, Nataša", year = "2016", abstract = "alpha-Amylase from Bacillus licheniformis ATCC 9945a (BliAmy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of BliAmy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), BliAmy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. BliAmy presents interesting performances on highly concentrated solid starch and could be of value for starch-consuming industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase", volume = "100", number = "6", pages = "2709-2719", doi = "10.1007/s00253-015-7101-4" }
Šokarda Slavić, M., Pešić, M., Vujčić, Z.,& Božić, N.. (2016). Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase. in Applied Microbiology and Biotechnology Springer, New York., 100(6), 2709-2719. https://doi.org/10.1007/s00253-015-7101-4
Šokarda Slavić M, Pešić M, Vujčić Z, Božić N. Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase. in Applied Microbiology and Biotechnology. 2016;100(6):2709-2719. doi:10.1007/s00253-015-7101-4 .
Šokarda Slavić, Marinela, Pešić, Milja, Vujčić, Zoran, Božić, Nataša, "Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase" in Applied Microbiology and Biotechnology, 100, no. 6 (2016):2709-2719, https://doi.org/10.1007/s00253-015-7101-4 . .