Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays
Само за регистроване кориснике
2013
Аутори
Gorjanović, Stanislava Ž.Alvarez-Suarez, Jose Miguel
Novaković, Miroslav
Pastor, Ferenc T.
Pezo, Lato
Battino, Maurizio
Suznjevic, Desanka Z.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxid...ant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.
Кључне речи:
Analytical method / Food analysis / Honey / Honey constituents / Food composition / Phenolics / Reducing sugars / Antioxidant activity / Hydrogen peroxide scavenge / DC polarography / Anodic current / Honey adulИзвор:
Journal of Food Composition and Analysis, 2013, 30, 1, 13-18Издавач:
- Academic Press Inc Elsevier Science, San Diego
Финансирање / пројекти:
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
- Утицај сорте и услова гајења на садржај биоактивних компоненти јагодастог и коштичавог воћа и добијање биолошки вредних производа побољшаним и новим технологијама (RS-MESTD-Technological Development (TD or TR)-31093)
DOI: 10.1016/j.jfca.2012.12.004
ISSN: 0889-1575
WoS: 000317799500003
Scopus: 2-s2.0-84873531795
Институција/група
IHTMTY - JOUR AU - Gorjanović, Stanislava Ž. AU - Alvarez-Suarez, Jose Miguel AU - Novaković, Miroslav AU - Pastor, Ferenc T. AU - Pezo, Lato AU - Battino, Maurizio AU - Suznjevic, Desanka Z. PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1357 AB - Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant assays commonly applied in analysis of honey and can serve as an indicator of honey quality. PB - Academic Press Inc Elsevier Science, San Diego T2 - Journal of Food Composition and Analysis T1 - Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays VL - 30 IS - 1 SP - 13 EP - 18 DO - 10.1016/j.jfca.2012.12.004 ER -
@article{ author = "Gorjanović, Stanislava Ž. and Alvarez-Suarez, Jose Miguel and Novaković, Miroslav and Pastor, Ferenc T. and Pezo, Lato and Battino, Maurizio and Suznjevic, Desanka Z.", year = "2013", abstract = "Hydrogen peroxide scavenging (HPS) activity of honey of different floral sources and its constituents such as predominant honey flavonoids, phenolic acids, amino and organic acids, and carbohydrates have been assessed by direct current (DC) polarographic assay. The assay was based on decrease of anodic current of hydrogen peroxide complex, formed in alkaline solution, at the potential of mercury dissolution. High correlations between honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the relative antioxidant capacity index, were obtained. Statistical evaluation by ANOVA and F-test further confirmed the assay validity. The results for individual compounds showed that HPS activity of honey reflects an integrated action of a wide range of constituents, both phenolics and non-phenolics. The polarographic assay applied is a fast, reliable and low cost alternative to spectrophotometric antioxidant assays commonly applied in analysis of honey and can serve as an indicator of honey quality.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Journal of Food Composition and Analysis", title = "Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays", volume = "30", number = "1", pages = "13-18", doi = "10.1016/j.jfca.2012.12.004" }
Gorjanović, S. Ž., Alvarez-Suarez, J. M., Novaković, M., Pastor, F. T., Pezo, L., Battino, M.,& Suznjevic, D. Z.. (2013). Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. in Journal of Food Composition and Analysis Academic Press Inc Elsevier Science, San Diego., 30(1), 13-18. https://doi.org/10.1016/j.jfca.2012.12.004
Gorjanović SŽ, Alvarez-Suarez JM, Novaković M, Pastor FT, Pezo L, Battino M, Suznjevic DZ. Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. in Journal of Food Composition and Analysis. 2013;30(1):13-18. doi:10.1016/j.jfca.2012.12.004 .
Gorjanović, Stanislava Ž., Alvarez-Suarez, Jose Miguel, Novaković, Miroslav, Pastor, Ferenc T., Pezo, Lato, Battino, Maurizio, Suznjevic, Desanka Z., "Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays" in Journal of Food Composition and Analysis, 30, no. 1 (2013):13-18, https://doi.org/10.1016/j.jfca.2012.12.004 . .