Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract
2013
Autori
Miladinovic, Dragoljub L.Ilic, Budimir S.
Najman, Stevo J.
Cvetković, Olga
Šajnović, Aleksandra
Miladinović, Marija D.
Nikolic, Nikola D.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have... an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.
Ključne reči:
Astragalus onobrychis / Antioxidant enzymes / Reactive oxygen species / Antioxidant capacity / ChemiluminescenceIzvor:
Central European Journal of Chemistry, 2013, 11, 2, 123-132Izdavač:
- Versita, Warsaw
Finansiranje / projekti:
DOI: 10.2478/s11532-012-0163-6
ISSN: 1895-1066
WoS: 000311778700001
Scopus: 2-s2.0-84875710644
Institucija/grupa
IHTMTY - JOUR AU - Miladinovic, Dragoljub L. AU - Ilic, Budimir S. AU - Najman, Stevo J. AU - Cvetković, Olga AU - Šajnović, Aleksandra AU - Miladinović, Marija D. AU - Nikolic, Nikola D. PY - 2013 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1320 AB - The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress. PB - Versita, Warsaw T2 - Central European Journal of Chemistry T1 - Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract VL - 11 IS - 2 SP - 123 EP - 132 DO - 10.2478/s11532-012-0163-6 ER -
@article{ author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Najman, Stevo J. and Cvetković, Olga and Šajnović, Aleksandra and Miladinović, Marija D. and Nikolic, Nikola D.", year = "2013", abstract = "The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.", publisher = "Versita, Warsaw", journal = "Central European Journal of Chemistry", title = "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract", volume = "11", number = "2", pages = "123-132", doi = "10.2478/s11532-012-0163-6" }
Miladinovic, D. L., Ilic, B. S., Najman, S. J., Cvetković, O., Šajnović, A., Miladinović, M. D.,& Nikolic, N. D.. (2013). Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. in Central European Journal of Chemistry Versita, Warsaw., 11(2), 123-132. https://doi.org/10.2478/s11532-012-0163-6
Miladinovic DL, Ilic BS, Najman SJ, Cvetković O, Šajnović A, Miladinović MD, Nikolic ND. Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. in Central European Journal of Chemistry. 2013;11(2):123-132. doi:10.2478/s11532-012-0163-6 .
Miladinovic, Dragoljub L., Ilic, Budimir S., Najman, Stevo J., Cvetković, Olga, Šajnović, Aleksandra, Miladinović, Marija D., Nikolic, Nikola D., "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract" in Central European Journal of Chemistry, 11, no. 2 (2013):123-132, https://doi.org/10.2478/s11532-012-0163-6 . .