Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique
Authors
Pejić, NatašaMaksimović, Jelena
Blagojević, Slavica
Anić, Slobodan
Čupić, Željko
Kolar-Anić, Ljiljana
Article (Published version)
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Simple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO3 and H2SO4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift Delta E-m, and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 x 10(-5)-2.68 x 10(-4) mol L-1 and 2.98 x 10(-5)-3.58 x 10(-4) mol L-1, respectively. The methods have an excellent sample throughput of 30 samples h(-1) (method A) and 7 samples h(-1) (metho...d B) with the sensitivity determined to be 1.1 x 10(-5) mol L-1 (method A) and 8.9 x 10(-6) mol L-1 (method B) as well as the precision RSD LT = 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail.
Keywords:
uric acid / perturbation technique / Bray-Liebhafsky oscillatory reaction / Dushman reaction / urineSource:
Journal of the Brazilian Chemical Society, 2012, 23, 8, 1450-1459Publisher:
- Sociedade Brasileira de Química
Funding / projects:
- Dynamics of nonlinear physicochemical and biochemical systems with modeling and predicting of their behavior under nonequilibrium conditions (RS-172015)
- Nanostructured Functional and Composite Materials in Catalytic and Sorption Processes (RS-45001)
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IHTMTY - JOUR AU - Pejić, Nataša AU - Maksimović, Jelena AU - Blagojević, Slavica AU - Anić, Slobodan AU - Čupić, Željko AU - Kolar-Anić, Ljiljana PY - 2012 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1130 AB - Simple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO3 and H2SO4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift Delta E-m, and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 x 10(-5)-2.68 x 10(-4) mol L-1 and 2.98 x 10(-5)-3.58 x 10(-4) mol L-1, respectively. The methods have an excellent sample throughput of 30 samples h(-1) (method A) and 7 samples h(-1) (method B) with the sensitivity determined to be 1.1 x 10(-5) mol L-1 (method A) and 8.9 x 10(-6) mol L-1 (method B) as well as the precision RSD LT = 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail. PB - Sociedade Brasileira de Química T2 - Journal of the Brazilian Chemical Society T1 - Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique VL - 23 IS - 8 SP - 1450 EP - 1459 UR - https://hdl.handle.net/21.15107/rcub_cer_1130 ER -
@article{ author = "Pejić, Nataša and Maksimović, Jelena and Blagojević, Slavica and Anić, Slobodan and Čupić, Željko and Kolar-Anić, Ljiljana", year = "2012", abstract = "Simple and reliable novel methods for the determination of uric acid (UA) are proposed and validated. For quantitative determination of UA, two matrices were used: the Bray-Liebhafsky (BL) oscillatory reaction in a stable non-equilibrium stationary state close to the bifurcation point (method A) as well as, the BL non-oscillating subsystem (mixture KIO3 and H2SO4), i.e., Dushman reaction (DR) in a steady state (method B). The proposed methods are optimized in a continuously fed well stirred tank reactor (CSTR) and applied with excellent results in the determination of UA in human urine samples. The linear relationship between maximal potential shift Delta E-m, and both the logarithm of the UA concentration (procedure A) and UA concentration (procedure B) is obtained in the concentration range 2.98 x 10(-5)-2.68 x 10(-4) mol L-1 and 2.98 x 10(-5)-3.58 x 10(-4) mol L-1, respectively. The methods have an excellent sample throughput of 30 samples h(-1) (method A) and 7 samples h(-1) (method B) with the sensitivity determined to be 1.1 x 10(-5) mol L-1 (method A) and 8.9 x 10(-6) mol L-1 (method B) as well as the precision RSD LT = 3.4% for both methods. Some aspects of the possible mechanism of UA action on the BL oscillating and Duschman non-oscillating reaction systems are discussed in detail.", publisher = "Sociedade Brasileira de Química", journal = "Journal of the Brazilian Chemical Society", title = "Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique", volume = "23", number = "8", pages = "1450-1459", url = "https://hdl.handle.net/21.15107/rcub_cer_1130" }
Pejić, N., Maksimović, J., Blagojević, S., Anić, S., Čupić, Ž.,& Kolar-Anić, L.. (2012). Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique. in Journal of the Brazilian Chemical Society Sociedade Brasileira de Química., 23(8), 1450-1459. https://hdl.handle.net/21.15107/rcub_cer_1130
Pejić N, Maksimović J, Blagojević S, Anić S, Čupić Ž, Kolar-Anić L. Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique. in Journal of the Brazilian Chemical Society. 2012;23(8):1450-1459. https://hdl.handle.net/21.15107/rcub_cer_1130 .
Pejić, Nataša, Maksimović, Jelena, Blagojević, Slavica, Anić, Slobodan, Čupić, Željko, Kolar-Anić, Ljiljana, "Kinetic Analytical Method for Determination of Uric Acid in Human Urine using Analyte Pulse Perturbation Technique" in Journal of the Brazilian Chemical Society, 23, no. 8 (2012):1450-1459, https://hdl.handle.net/21.15107/rcub_cer_1130 .