This article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. A bacterial consortium, consisting of strains from the genera Pseudomonas, Achromobacter, Bacillus and Micromonospora, was isolated from a petroleum sludge sample and characterized. The addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory conditions. The extent of biodegradation was monitored by the gravimetric method and analysis of the residual oil by gas chromatography. Over a 12-week experiment, the achieved degree of TPH (total petroleum hydrocarbon) degradation amounted to 82-88% in the petroleum sludge and 86-91% in the polluted soil. Gas chromatography-mass spectrometry was utilized to determine the biodegradability and degradation rates of n-alkanes, isoprenoids, steranes, diasteranes and terpanes. Complete degradation of th...e n-alkanes and isoprenoids fractions occurred in both the samples. In addition, the intensities of the peaks corresponding to tricyclic terpenes and homohopanes were decreased, while significant changes were also observed in the distribution of diasteranes and steranes.
TY - JOUR
AU - Gojgić-Cvijović, Gordana
AU - Milić, Jelena
AU - Šolević Knudsen, Tatjana
AU - Beškoski, Vladimir
AU - Ilić, Mila
AU - Đokić, Lidija
AU - Narancic, T. M.
AU - Vrvić, Miroslav
PY - 2012
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/999
AB - This article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. A bacterial consortium, consisting of strains from the genera Pseudomonas, Achromobacter, Bacillus and Micromonospora, was isolated from a petroleum sludge sample and characterized. The addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory conditions. The extent of biodegradation was monitored by the gravimetric method and analysis of the residual oil by gas chromatography. Over a 12-week experiment, the achieved degree of TPH (total petroleum hydrocarbon) degradation amounted to 82-88% in the petroleum sludge and 86-91% in the polluted soil. Gas chromatography-mass spectrometry was utilized to determine the biodegradability and degradation rates of n-alkanes, isoprenoids, steranes, diasteranes and terpanes. Complete degradation of the n-alkanes and isoprenoids fractions occurred in both the samples. In addition, the intensities of the peaks corresponding to tricyclic terpenes and homohopanes were decreased, while significant changes were also observed in the distribution of diasteranes and steranes.
PB - Springer, New York
T2 - Biodegradation
T1 - Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study
VL - 23
IS - 1
SP - 1
EP - 14
DO - 10.1007/s10532-011-9481-1
ER -
@article{
author = "Gojgić-Cvijović, Gordana and Milić, Jelena and Šolević Knudsen, Tatjana and Beškoski, Vladimir and Ilić, Mila and Đokić, Lidija and Narancic, T. M. and Vrvić, Miroslav",
year = "2012",
abstract = "This article presents a study of the efficiency and degradation pattern of samples of petroleum sludge and polluted sandy soil from an oil refinery. A bacterial consortium, consisting of strains from the genera Pseudomonas, Achromobacter, Bacillus and Micromonospora, was isolated from a petroleum sludge sample and characterized. The addition of nitrogen and phosphorus nutrients and a chemical surfactant to both the samples and bioaugmentation to the soil sample were applied under laboratory conditions. The extent of biodegradation was monitored by the gravimetric method and analysis of the residual oil by gas chromatography. Over a 12-week experiment, the achieved degree of TPH (total petroleum hydrocarbon) degradation amounted to 82-88% in the petroleum sludge and 86-91% in the polluted soil. Gas chromatography-mass spectrometry was utilized to determine the biodegradability and degradation rates of n-alkanes, isoprenoids, steranes, diasteranes and terpanes. Complete degradation of the n-alkanes and isoprenoids fractions occurred in both the samples. In addition, the intensities of the peaks corresponding to tricyclic terpenes and homohopanes were decreased, while significant changes were also observed in the distribution of diasteranes and steranes.",
publisher = "Springer, New York",
journal = "Biodegradation",
title = "Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study",
volume = "23",
number = "1",
pages = "1-14",
doi = "10.1007/s10532-011-9481-1"
}
Gojgić-Cvijović, G., Milić, J., Šolević Knudsen, T., Beškoski, V., Ilić, M., Đokić, L., Narancic, T. M.,& Vrvić, M.. (2012). Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. in Biodegradation
Springer, New York., 23(1), 1-14.
https://doi.org/10.1007/s10532-011-9481-1
Gojgić-Cvijović G, Milić J, Šolević Knudsen T, Beškoski V, Ilić M, Đokić L, Narancic TM, Vrvić M. Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. in Biodegradation. 2012;23(1):1-14.
doi:10.1007/s10532-011-9481-1 .
Gojgić-Cvijović, Gordana, Milić, Jelena, Šolević Knudsen, Tatjana, Beškoski, Vladimir, Ilić, Mila, Đokić, Lidija, Narancic, T. M., Vrvić, Miroslav, "Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study" in Biodegradation, 23, no. 1 (2012):1-14,
https://doi.org/10.1007/s10532-011-9481-1 . .
