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Produkcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-ai

dc.creatorRikalović, Milena
dc.creatorGojgić-Cvijović, Gordana
dc.creatorVrvić, Miroslav
dc.creatorKaradžić, Ivanka
dc.date.accessioned2019-01-30T17:30:16Z
dc.date.available2019-01-30T17:30:16Z
dc.date.issued2012
dc.identifier.issn0352-5139
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/998
dc.description.abstractThe production and characteristics of rhamnolipid biosurfactant obtained by the strain Pseudomonas aeruginosa san-ai were investigated. With regard to the carbon and nitrogen sources, several media were tested to enhance the production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sunflower oil as a source of carbon and mineral ammonium chloride and peptone as nitrogen sources greatly improved the production of rhamnolipid, from 0.15 on basic PPAS (C/N ratio 4.0) to 3 g L-1 on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing the effect of three factors, i.e., temperature, concentration of carbon and nitrogen source (mass %), in the optimized PPAS medium on the production of rhamnolipid. The isolated rhamnolipids were characterized by infrared (IR) spectroscopy and electrospray ionization mass spectrometry (ESI-MS). The IR spectra confirmed that the isolated compound corresponded to the rhamnolipid structure, whereas MS indicated that the isolated preparation was a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic and di-rhamno-di-lipidic congeners.en
dc.description.abstractU ovom radu je opisana produkcija i karakterizacija ramnolipidnog biosurfaktanta soja Pseudomonas aeruginosa san-ai. Ispitan je uticaj različitih izvora ugljenika i azota na produkciju ramnolipida. Podloga proteoze pepton-amonijum soli sa ograničenom količinom fosfata (PPAS) sa suncokretovim uljem kao izvorom ugljenika i amonijum-hloridom i peptonom kao izvorom azota, znatno povećava produkciju ramnolipida, od 0,15, na PPAS sa standardnim sastavom (odnos C/N 4,0), do 3 g L-1 na optimizovanoj PPAS podlozi (odnos C/N 7,7). Metodom planiranog eksperimenta (response surface methodology) ispitan je efekat tri parametra: temperature, koncentracije izvora ugljenika i azota na optimizovanoj podlozi. Karakterizacija izolovanog preparata ramnolipida urađena je pomoću IR i ESI-MS analiza. IR analiza je potvrdila prisustvo strukturnih elemenata karakterističnih za ramnolipide. MS analiza pokazala je da reč o smeši u kojoj su prisutne mono-ramno-monolipidne, di-ramno-mono-lipidne i di-ramno-di-lipidne komponente.sr
dc.publisherSerbian Chemical Society
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectrhamnolipidsen
dc.subjectPseudomonas aeruginosaen
dc.subjectrenewable sourcesen
dc.titleProduction and characterization of rhamnolipids from Pseudomonas aeruginosa san-aien
dc.titleProdukcija i karakterizacija ramnolipida soja Pseudomonas aeruginosa san-aisr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractКарадзиц, Иванка; Врвиц, Мирослав М.; Гојгић Цвијовић, Гордана; Рикаловиц, Милена Г.; Продукција и карактеризација рамнолипида соја Псеудомонас аеругиноса сан-аи; Продукција и карактеризација рамнолипида соја Псеудомонас аеругиноса сан-аи;
dc.citation.volume77
dc.citation.issue1
dc.citation.spage27
dc.citation.epage42
dc.citation.other77(1): 27-42
dc.citation.rankM23
dc.identifier.doi10.2298/JSC110211156R
dc.identifier.rcubConv_2739
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/9567/996.pdf
dc.identifier.scopus2-s2.0-84858652979
dc.identifier.wos000300386100004
dc.type.versionpublishedVersion


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